CN102253009B - Detection method of edible fungus specimen preserving liquid - Google Patents
Detection method of edible fungus specimen preserving liquid Download PDFInfo
- Publication number
- CN102253009B CN102253009B CN201110064867A CN201110064867A CN102253009B CN 102253009 B CN102253009 B CN 102253009B CN 201110064867 A CN201110064867 A CN 201110064867A CN 201110064867 A CN201110064867 A CN 201110064867A CN 102253009 B CN102253009 B CN 102253009B
- Authority
- CN
- China
- Prior art keywords
- liquid
- edible fungi
- sample
- preserved
- detection method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
A detection method of an edible fungus specimen preserving liquid relates to the field of edible fungus specimen. According to the detection method, a sporocarp of an edible fungus is cut into cubes and immersed into a preserving liquid in a test tube; light transmittances of a preserving liquid immersed with an edible fungus specimen and of a preserving liquid without immersed edible fungus specimen are detected according to a corresponding detector; then light transmittances of different preserving liquids are calculated through comparison, so as to obtain an optimum edible fungus specimen preserving liquid. The detection method is sensitive, rapid, simply operated and at low costs, besides the detected data is accurate with good repeatability. In addition, a complete set of systematic specimen preserving liquid detection system is established, while natural color of the edible fungus specimen is preserved effectively.
Description
[technical field]
The present invention relates to edible fungi sample field, especially relate to the detection method that a kind of edible fungi sample is preserved liquid.
[background technology]
Known; The research of preserving liquid about plant specimen is comparatively extensive; Though the existing sample preservation liquid that is used to preserve various plant specimens that successfully works out; But the clarity detection of preserving liquid for sample but is to rely on naked eyes to judge always, wherein just comprises the detection of the edible fungi sample being preserved liquid; Because the color of fructification is one of key character of edible fungi; And the preservation of large edible bacterium sample is the prerequisite that is used for its Study of recognition; The clarity that is sample preservation liquid is the important prerequisite condition that guarantees that fruit-body color can intuitively appear; Carry out the judgement on the sense organ if only rely on naked eyes to come that sample is preserved liquid, not only can accurately system not detect the clarity that the edible fungi sample is preserved liquid, but also can't accurately screen the quality of edible fungi sample preservation liquid; Therefore be unfavorable for that picking out best edible fungi sample preserves liquid, makes the fructification sample show original color looks exactly; At present; The clearer identification of people for ease and understand various edible fungis; Edible fungi sample as one of audiovisual aids is widely used in the scientific research and education; But, the development of edible fungi sample preservation technology is restricted owing to there is not effectively to be directed against the detection method that the edible fungi sample is preserved liquid.
[summary of the invention]
In order to overcome the deficiency in the background technology, the invention provides a kind of easy and simple to handlely, and can preserve the detection method that edible fungi sample that liquid quick and precisely detect is preserved liquid to various edible fungi samples.
For realizing the foregoing invention purpose, the present invention adopts following technical scheme:
A kind of edible fungi sample is preserved the detection method of liquid, and described detection method is:
A, in many identical test tubes of specification, pour the preservation liquid of equivalent respectively into;
B, keep a test tube, again with after the even stripping and slicing of fruit body of edible fungi sample respectively equivalent join in the residue test tube, in vitro put into glass bead to what sample was housed then, and with glass bead sample pressed to immerse fully and preserve liquid;
C, seal the mouth of pipe of all test tubes respectively after, all test tubes were preserved 10 days;
D, after 10 days, detect in every test tube the penetrability of preserving liquid respectively through the pick-up unit that is provided with illuminometer;
E, the penetrability that will not soak the preservation liquid of edible fungi sample are decided to be A; There is the penetrability of the preservation liquid of sample to be decided to be B immersion; Pass through formula then:
calculates the transmittance that each immersion has the preservation liquid of edible fungi sample, again with the average transmittance that draws this preservation liquid after the transmittance addition that draws divided by the test tube number that the edible fungi sample is housed;
F, other edible fungi samples that need detect are preserved liquid undertaken by above-mentioned steps respectively; And calculate the average transmittance that each preserves liquid; Average transmittance with various edible fungi samples preservation liquid compares then, and the preservation liquid preservation effect that average transmittance is more little is good more.
Described edible fungi sample is preserved the detection method of liquid, and described pick-up unit comprises seal box, digital display type illuminometer, cuvette and LED spotlight; Described seal box is provided with the sealing lid, and is provided with digital display type illuminometer, cuvette and LED spotlight in the box; Described digital display type illuminometer comprises photodetector and reading display; Described photodetector fixedly is located at the interior side of box of seal box, and is connected through the data line of the hermetic penetrant seal box box body reading display outside with being located at seal box; Described LED spotlight is white light modulation, is fixedly mounted on opposite side in the box of seal box, and the light of LED spotlight penetrates the front of directive photodetector behind the cuvette; Extend the seal box outside behind the box body of the power lead hermetic penetrant seal box of LED spotlight.
Described edible fungi sample is preserved the detection method of liquid, and described test tube is at least three.
Described edible fungi sample is preserved the detection method of liquid, and described fruit body of edible fungi sample evenly is cut into the bulk of the long 6mm of rib.
Described edible fungi sample is preserved the detection method of liquid, the fruit body of edible fungi sample after the said stripping and slicing by 50 ~ 100g respectively equivalent join and remain in the test tube, and the fruit body of edible fungi sample in every test tube accounts for 1/2 of this test tube capacity.
Described edible fungi sample is preserved the detection method of liquid, and described all test tube mouths of pipe with the two layers of plastic thin films sealing, were preserved 10 days in 20～40 ℃ respectively then.
Because adopt described technical scheme, the present invention has following beneficial effect:
Edible fungi sample of the present invention preserves that the detection method of liquid can system preserves liquid to various edible fungi samples and detects fast and accurately; Thereby can accurately screen the quality of edible fungi sample preservation liquid, be beneficial to the best of picking out the edible fungi sample and preserve liquid; Described detection method not only sensitive quick, easy and simple to handle, cost is lower; And detected data are accurate and good reproducibility; Effectively preserving on the natural color basis of edible fungi sample simultaneously; Set up the sample of a cover holonomic system and preserved the liquid detection architecture, thereby when preserving liquid, strengthened the discriminating and the sight of edible fungi sample through clarity screening edible fungi sample; Detection method of the present invention is preserved the blank that technical elements has been filled up detection edible fungi sample preservation liquid in teaching and scientific research and sample, has effectively promoted the development of edible fungi sample preservation technology.
[description of drawings]
Fig. 1 is a seal box synoptic diagram of the present invention;
Fig. 2 is a diagrammatic cross-section of the present invention.
Among the figure: 1, seal box; 2, cuvette; 3, LED spotlight; 4, sealing lid; 5, photodetector; 6, data line; 7, reading display; 8, power lead.
[embodiment]
Can explain the present invention in more detail through following embodiment, open the object of the invention is intended to protect all variations and the improvement in the scope of the invention, and the present invention is not limited to following embodiment;
In conjunction with the detection method that accompanying drawing 1 described a kind of edible fungi sample is preserved liquid, described detection method is:
A, in many identical test tubes of specification, pour the preservation liquid of equivalent respectively into, promptly guaranteed and to have carried out the equivalent contrast to preserving liquid, effectively reduced error; As required, preferably use the standard test tube of 15 * 150mm, and can select the quantity of test tube as required, but will select three test tubes at least,, guarantee the accuracy that detects so that compare reference;
The test tube of preserving liquid is equipped with in b, one of reservation earlier; Again with after the even stripping and slicing of fruit body of edible fungi sample respectively equivalent join in the remaining test tube; In vitro put into glass bead to what the fruit body of edible fungi sample was housed then; And with glass bead the fruit body of edible fungi sample is pressed fully and immerse to preserve liquid; Thereby when the fruit body of edible fungi sample fully is soaked in the preservation liquid, utilize the glass bead that can not influence preservation liquid liquid matter to guarantee accurately to detect the penetrability of soaking the preservation liquid that the fruit body of edible fungi sample is arranged; For the benefit of soak, join again in the corresponding test tube after can described fruit body of edible fungi sample evenly being cut into the bulk of the long 6mm of rib; For simplifying the operation, can with the fruit body of edible fungi sample after the stripping and slicing by the weight of 50 ~ 100g respectively equivalent join in the residue test tube, and preserved the fruit body of edible fungi sample that liquid soaks in every test tube and accounted for 1/2 of this test tube capacity;
C, seal the mouth of pipe of all test tubes respectively, promptly effectively avoided external substance to get into test tube and had influence on the liquid matter of preserving liquid, the test tube after will seal was then preserved 10 days, and storage temperature at this moment is preferably 20～40 ℃; For ease of operation, can utilize bungee respectively the mouth of pipe of all test tubes to be sealed with two layers of plastic thin films;
D, after 10 days, detect in every test tube the penetrability of preserving liquid respectively, promptly detect the penetrability numerical value of preserving liquid in every test tube through the illuminometer in the pick-up unit through the pick-up unit that is provided with illuminometer;
E, the penetrability that will not soak the preservation liquid of fruit body of edible fungi sample are decided to be A; There is the penetrability of the preservation liquid of fruit body of edible fungi sample to be decided to be B immersion; Pass through formula then:
calculates the transmittance that each immersion has the preservation liquid of fruit body of edible fungi sample, again with the average transmittance that draws this kind preservation liquid after the transmittance addition that draws divided by the test tube number that the fruit body of edible fungi sample is housed;
F, other fruit body of edible fungi samples that need detect are preserved liquid undertaken by above-mentioned steps respectively; And calculate the average transmittance that each preserves liquid; The average transmittance of inciting somebody to action detected various fruit body of edible fungi samples preservation liquid then compares, and the preservation liquid preservation effect that then average transmittance numerical value is more little is good more.
Described edible fungi sample is preserved the detection method of liquid, and described pick-up unit comprises seal box 1, digital display type illuminometer, cuvette 2 and LED spotlight 3; Described seal box 1 is provided with sealing lid 4, and is provided with digital display type illuminometer, cuvette 2 and LED spotlight 3 in seal box 1 box; Described digital display type illuminometer comprises photodetector 5 and reading display 7; Described photodetector 5 fixedly is located at the interior side of box of seal box 1, and is connected with the reading display 7 that is located at seal box 1 outside through the data line 6 of hermetic penetrant seal box 1 box body; Described LED spotlight 3 is white light modulation; Be fixedly mounted on the interior opposite side of box of seal box 1; And the light of LED spotlight 3 penetrates the front of cuvette 2 back directive photodetectors 5, adorns the penetrability of preserving liquid in the cuvette 2 thereby utilize photodetector 5 accurately to detect; Extend seal box 1 outside behind the box body of the power lead 8 hermetic penetrant seal boxes 1 of LED spotlight 3.
When the described a kind of edible fungi sample of embodiment of the present invention is preserved the detection method of liquid; Only need to detect the penetrability of preserving liquid by described step; Pass through formula then:
calculates the average transmittance of various preservation liquid, and can access best edible fungi sample preservation liquid after comparing; Wherein, When detecting the penetrability of preserving liquid; To earlier the preservation liquid in the test tube that needs to detect be poured in the cuvette 2 in seal box 1 box; And then close the sealing lid 4 of seal box 1, and open LED spotlight 3, utilize photodetector 5 in the illuminometer to receive LED spotlights 3 and see through the light of penetrating behind the cuvette 2; And through the reading display 7 in the illuminometer detected preservation liquid penetrability numerical value is shown, promptly utilize described pick-up unit that the preservation liquid in each test tube is carried out printing opacity respectively and detect the penetrability that promptly can draw the preservation liquid that is detected.
Not having specifically described part in the present invention's explanation is prior art, so carefully do not state.
Claims (6)
1. an edible fungi sample is preserved the detection method of liquid, and it is characterized in that: described detection method is:
A, in many identical test tubes of specification, pour the preservation liquid of equivalent respectively into;
B, keep a test tube, again with after the even stripping and slicing of fruit body of edible fungi sample respectively equivalent join in the residue test tube, in vitro put into glass bead to what sample was housed then, and with glass bead sample pressed to immerse fully and preserve liquid;
C, seal the mouth of pipe of all test tubes respectively after, all test tubes were preserved 10 days;
D, after 10 days, detect in every test tube the penetrability of preserving liquid respectively through the pick-up unit that is provided with illuminometer;
E, the penetrability that will not soak the preservation liquid of edible fungi sample are decided to be A; There is the penetrability of the preservation liquid of sample to be decided to be B immersion; Pass through formula then:
calculates the transmittance that each immersion has the preservation liquid of edible fungi sample, again with the average transmittance that draws this preservation liquid after the transmittance addition that draws divided by the test tube number that the edible fungi sample is housed;
F, other edible fungi samples that need detect are preserved liquid undertaken by above-mentioned steps respectively; And calculate the average transmittance that each preserves liquid; Average transmittance with various edible fungi samples preservation liquid compares then, and the preservation liquid preservation effect that average transmittance is more little is good more.
2. edible fungi sample according to claim 1 is preserved the detection method of liquid, and it is characterized in that: described pick-up unit comprises seal box (1), digital display type illuminometer, cuvette (2) and LED spotlight (3); Described seal box (1) is provided with sealing lid (4), and is provided with digital display type illuminometer, cuvette (2) and LED spotlight (3) in seal box (1) box; Described digital display type illuminometer comprises photodetector (5) and reading display (7); Described photodetector (5) fixedly is located at the interior side of box of seal box (1), and is connected through the data line (6) of hermetic penetrant seal box (1) the box body reading display (7) outside with being located at seal box (1); Described LED spotlight (3) is white light modulation, be fixedly mounted on the interior opposite side of box of seal box (1), and the light of LED spotlight (3) penetrates the front of cuvette (2) back directive photodetector (5); Extend seal box (1) outside behind the box body of power lead (8) the hermetic penetrant seal box (1) of LED spotlight (3).
3. edible fungi sample according to claim 1 is preserved the detection method of liquid, and it is characterized in that: described test tube is at least three.
4. edible fungi sample according to claim 1 is preserved the detection method of liquid, and it is characterized in that: described fruit body of edible fungi sample evenly is cut into the bulk of the long 6mm of rib.
5. edible fungi sample according to claim 1 is preserved the detection method of liquid; It is characterized in that: the fruit body of edible fungi sample after the said stripping and slicing by 50 ~ 100g respectively equivalent join in the residue test tube, and the fruit body of edible fungi sample in every test tube accounts for 1/2 of this test tube capacity.
6. edible fungi sample according to claim 1 is preserved the detection method of liquid, it is characterized in that: described all test tube mouths of pipe with the two layers of plastic thin films sealing, were preserved 10 days in 20～40 ℃ respectively then.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110064867A CN102253009B (en) | 2011-03-17 | 2011-03-17 | Detection method of edible fungus specimen preserving liquid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110064867A CN102253009B (en) | 2011-03-17 | 2011-03-17 | Detection method of edible fungus specimen preserving liquid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102253009A CN102253009A (en) | 2011-11-23 |
| CN102253009B true CN102253009B (en) | 2012-08-29 |
Family
ID=44980399
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110064867A Expired - Fee Related CN102253009B (en) | 2011-03-17 | 2011-03-17 | Detection method of edible fungus specimen preserving liquid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102253009B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102676389B (en) * | 2012-01-04 | 2014-04-23 | 河南科技大学 | Edible fungi strain preservation method and device facilitating taking strains at any time |
| CN104186461A (en) * | 2014-08-19 | 2014-12-10 | 中华全国供销合作总社昆明食用菌研究所 | Preparation method of edible fungus specimen |
| CN109856089A (en) * | 2018-11-09 | 2019-06-07 | 合肥诺森医学检验有限公司 | A kind of quality determining method of cell-preservation liquid |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2282880B (en) * | 1993-10-18 | 1997-07-23 | Welsh Water Enterprises Ltd | Apparatus for measuring characteristics of a liquid |
| CN101559144B (en) * | 2008-04-17 | 2012-11-28 | 王守东 | Oral Tonglin Hushen capsule for curing urethritis, cystitis, acute pyelonephritis and chronic pyelonephritis and preparation method thereof |
| CN101871889B (en) * | 2010-06-02 | 2012-11-28 | 张琪 | Liquid sample detection device utilizing LED light source |
-
2011
- 2011-03-17 CN CN201110064867A patent/CN102253009B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN102253009A (en) | 2011-11-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102253009B (en) | Detection method of edible fungus specimen preserving liquid | |
| CN110618116A (en) | Preparation method and application of intelligent indication label for visually detecting freshness of meat | |
| US4998824A (en) | System of fluid inspection and/or identification | |
| DK147007B (en) | PROCEDURE FOR DETERMINING MICRO-ORGANISMS AND APPARATUS FOR USING THE PROCEDURE | |
| CN205561369U (en) | Eat quality control and survey sample fridge | |
| CN102087221B (en) | Method for rapidly determining dissolved oxygen in water | |
| CN206540919U (en) | A kind of Cystatin C determines kit | |
| CN206750231U (en) | A kind of tank body vacuum-ness detecting device | |
| CN111417852A (en) | Inspection method in food packaging | |
| CN208206546U (en) | A kind of sampler convenient for Locale Holding water sample | |
| CN102654462A (en) | TMB (tetramethylbenzidine) residual chlorine comparator | |
| CN108896775A (en) | A kind of Portable egg white matter quantitative testing device and method | |
| CN105486625A (en) | Cell counting device and method based on Terahertz time-domain spectroscopy technology | |
| CN107334644B (en) | Container | |
| CN105572400A (en) | Sample reaction vessel and sample detection method | |
| CN203470360U (en) | Penicillin bottle vacuum on-line detector | |
| CN208632560U (en) | A kind of cell growth detection device | |
| CN206906241U (en) | A kind of double light source nutrient salt analyzer | |
| CN208109475U (en) | A kind of beer conducive to observation keeps away oxygen sampler | |
| CN209291116U (en) | A kind of water sample storage box | |
| CN205762414U (en) | A kind of aluminium foil sealing detection device | |
| CN206330886U (en) | A kind of Novel ATP fluorescence microbial rapid detection instrument | |
| CN205046135U (en) | Take temperature indicator's cell culture bottle | |
| CN212963856U (en) | Wine bottle leakage detector | |
| CN217359607U (en) | Device for detecting color difference of beer bottle |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120829 Termination date: 20150317 |
|
| EXPY | Termination of patent right or utility model |