CN102250758A - Enclosed reactor for microalgae culture - Google Patents

Enclosed reactor for microalgae culture Download PDF

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Publication number
CN102250758A
CN102250758A CN2011101128494A CN201110112849A CN102250758A CN 102250758 A CN102250758 A CN 102250758A CN 2011101128494 A CN2011101128494 A CN 2011101128494A CN 201110112849 A CN201110112849 A CN 201110112849A CN 102250758 A CN102250758 A CN 102250758A
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reactor
liquid
algae
gas
outlet
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CN102250758B (en
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张炳泉
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China Research Marine Biotechnology (shenzhen) Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02Photobioreactors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/24Recirculation of gas
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/26Conditioning fluids entering or exiting the reaction vessel

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
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  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention provides an enclosed reactor for microalgae culture; outlets of an algae liquid supplementing device and a carbon dioxide conveying system are communicated with an inlet of a nutrient liquid conveying system; an outlet of the nutrient liquid conveying system is communicated with an inlet of a mixed gas-liquid exchange device; an outlet of the mixed gas-liquid exchange device is communicated with an inlet of a reactor; a circulating port of the reactor is communicated with a circulating port of the mixed gas-liquid exchange device; an algae liquid outlet of the reactor is communicated with an algae liquid harvesting system; a movable valve is disposed on a side wall of the reactor. The reactor of the invention is three-dimensional, stereo, and multilayer; a circulating clapboard and an annular channel are disposed inside; the reactor is made of acrylic transparent rubber or glass; innovation is performed mainly in aspects of light source and CO2 supply; and problems in the following three aspects are solved: firstly, the omnidirectional supplement of the light source; secondly, more reasonable circulation flow of the algae liquid, and the supplement of CO2 required by algae; thirdly, the solution of the problems in aspects of maintenance, disassembly and washing in minimum time.

Description

A kind of both culturing microalgae closed reactor
Technical field
The present invention relates to a kind of both culturing microalgae closed reactor, belong to both culturing microalgae reactor technology field.
Background technology
Little algae has fast growth, the cycle is short, conversion of solar energy is high, comprehensive nutrition, be rich in advantages such as multiple high value biologically active substance, and become the important source of medicine, food and healthcare products, aquaculture organisms bait and fodder additives, be research and development object important in the marine biotechnology.At present, the large-scale industrial production of little algae the production models that adopt outdoor open pond formula, this production model more exist floor space big, produce unstable, pollution easily, luminous energy and CO 2Problems such as the not high and culture efficiency of utilization ratio is low have restricted seriously that little algae produces and the high speed development of little algae biotechnology industry.Other has a kind of employing canned, its advantage be for keeping algae kind purity pollution-free, can scale operation, but because cost height, maintenance are unpicked and washed, installation difficulty is big, infringement degree of one-tenth height of parts needs repairing and waits improper for a long time and be difficult to big area and promote.Domestic and international in recent years many scholars have carried out a series of research at this respect problem, especially utilize the research of the little algae of photoreactor high-efficient culture to obtain certain achievement.At present representative bioreactor mainly contains following several: 1. pipeline reactor; 2. plate-type reactor; 3. optical fiber reactor; 4. light-emitting diodes pipe reactor etc., these photoreactors are the trend of following little algae scale operation with optimal control and higher output and showed bright development prospect.But still there are some problems in practice in they,, gaseous interchange low as the efficiency of light energy utilization is not smooth etc., the high energy consumption of especially present pipeline bioreactor commonly used both at home and abroad, expensive, it is big to keep in repair, unpick and wash difficulty, the long subject matter that has become the development of restriction bioreactor of time consuming time.
Summary of the invention
The purpose of this invention is to provide a kind of both culturing microalgae closed reactor that can realize that high-density, high yield, less energy-consumption, low-cost cultivation require.
The objective of the invention is to be achieved through the following technical solutions:
The present invention includes: reactor, the algae liquid supplementing device, the carbonic acid gas delivery system, mix the gas-liquid switch, nutritive medium is carried system and algae liquid harvesting system, the outlet of algae liquid supplementing device and carbonic acid gas delivery system all carries the inlet of system to be connected with nutritive medium, nutritive medium carries the outlet of system to be connected with the inlet that mixes the gas-liquid switch, the outlet that mixes the gas-liquid switch is connected with the input aperture of reactor, the circulation port of reactor is connected with the circulation port that mixes the gas-liquid switch, the algae liquid delivery port of reactor is connected with algae liquid harvesting system, and a sidewall of reactor is provided with Movable valve.
Reactor of the present invention can be three-dimensional, three-dimensional, multilayer, in establish cycle partition and annular channel, adopt acrylic transparent adhesive tape or glass to make, can realize high-density, high yield, less energy-consumption, low-cost cultivate require in, mainly at light source, CO 2Supply respect is innovated, and has solved the problem of three aspects emphatically: the one, and light source is comprehensive to be replenished; The 2nd, the algae liquid recycle stream is moved more become rationally, replenish the required CO of algae simultaneously 2The 3rd, solve the problem of aspects such as can in the shortest time, keeping in repair, unpick and wash, and helped large-scale development.
The present invention adopts the three-dimensional structure, and sensitive surface extensively and comprehensively, all claims, remove clean convenient, only need existing duct type device ten/once time can finish.In microorganism algal grown process, adopt gas-liquid mixed (carbonic acid gas and algae nutrient solution), allow more accelerated development of algae grows.
Closed reactor major advantage of the present invention is: each three-dimensional vertical reactor is a unit, and the side is provided with Movable valve to be opened/closed, can open Movable valve when the needs repair and maintenance, cleans arbitrarily, conveniently saves time again, has reduced labour intensity.Can make up by many units as batch production production, more can save energy (power).
The present invention has broken through the pattern of existing closed conduit reactor, has kept the habit of circular flow, courageously adopts gas-liquid mixed, and effectively utilizes electromagnetic induction principle with water demineralization, and the growth of chlorella is multiplied.
Description of drawings
Fig. 1 is the structural representation of the both culturing microalgae closed reactor that provides of the specific embodiment of the invention.
Embodiment
A kind of both culturing microalgae closed reactor (see figure 1) that the specific embodiment of the invention provides, comprise: reactor 1, algae liquid supplementing device 16, carbonic acid gas delivery system 17, mix gas-liquid switch 18, nutritive medium is carried system 19 and algae liquid harvesting system 22, the outlet of algae liquid supplementing device 16 and carbonic acid gas delivery system 17 all carries the inlet of system 19 to be connected with nutritive medium, nutritive medium carries the outlet of system 19 to be connected with the inlet that mixes gas-liquid switch 18, the outlet that mixes gas-liquid switch 18 is connected with the input aperture 3 of reactor 1, the circulation port 4 of reactor 1 is connected with the circulation port 26 that mixes gas-liquid switch 18, the algae liquid delivery port 5 of reactor 1 is connected with algae liquid harvesting system 22, and a sidewall of reactor 1 is provided with Movable valve 2.
Further, also comprise magnetizing assembly 10, described magnetizing assembly 10 is serially connected on the pipeline between the input aperture 3 of mixing gas-liquid switch 18 and reactor 1.
Also comprise safety air vavle 11, described safety air vavle 11 is connected on the export pipeline that mixes gas-liquid switch 18.
Also comprise carbonic acid gas supplementary device 23, described carbonic acid gas supplementary device 23 is arranged on the bottom in the reactor 1.
Principle of work:
One, mix gas-liquid switch 18: its inlet is connected with instrument 13 with electric valve 12, and carries the outlet of system 19 to be connected with nutritive medium.Nutritive medium is carried the inlet of system 19 to be connected with instrument 15 by electric valve 14 with algae liquid replenishment system 16 and is entered, and simultaneously, is connected with instrument 25 by electric valve 24 with carbonic acid gas delivery system 17 and enters, and the mixing of realization algae liquid and carbonic acid gas exchanges.It enters in the reactor 1 from input aperture 3 through safety air vavle 11, magnetizing assembly 10, instrument 9, electric valve 8.
Two, algae liquid harvesting system 22: when little algae frond concentration of reactor 1 is 10 5-18 8Individual/ml, the delivery port 5 of algae liquid harvesting system 22 ligation devices 1, by electric valve 20 and instrument 21 enter that algae liquid harvesting system 22 effectively separates, broken wall, oven dry, packing become the chlorella raw material.
Three, circulating production system: the continuous merisis of chlorella in the reactor 1, owing to do not reach the regular hour, concentration in its algae does not reach desired concn as yet, unfavorable recovery, requirement enters its circulation port 4 through reactor 1, electric valve 6 and instrument 7 and mixes in the gas-liquid switch 18, enter reactor 1 after the gas-liquid mixed exchange once more, circulation running repeatedly.
Four, magnetization water quality: the growth algae through mixing the gas-liquid exchange is through the safety air vavle pipeline magnetizing assembly 10 of flowing through, and allows the water demineralization be active water quality, and algae can quicken merisis, and the quality of its algae is more outstanding.
Five, establish carbonic acid gas supplementary device 23 in the bottom of reactor 1, in reactor, to replenish carbonic acid gas as required.
Six, robot control system(RCS) can also be installed: according to the actual state of the variation of weather, temperature, flow velocity, be divided into multi-level running in 24 hours automatically, but not only energy-conservation but also accelerating growth.
The above; only be the preferable embodiment of the present invention; these embodiments all are based on the different implementations under the general idea of the present invention; and protection scope of the present invention is not limited thereto; anyly be familiar with those skilled in the art in the technical scope that the present invention discloses; the variation that can expect easily or replacement all should be encompassed within protection scope of the present invention.Therefore, protection scope of the present invention should be as the criterion with the protection domain of claims.

Claims (4)

1. both culturing microalgae closed reactor, it is characterized in that, comprise: reactor, the algae liquid supplementing device, the carbonic acid gas delivery system, mix the gas-liquid switch, nutritive medium is carried system and algae liquid harvesting system, the outlet of algae liquid supplementing device and carbonic acid gas delivery system all carries the inlet of system to be connected with nutritive medium, nutritive medium carries the outlet of system to be connected with the inlet that mixes the gas-liquid switch, the outlet that mixes the gas-liquid switch is connected with the input aperture of reactor, the circulation port of reactor is connected with the circulation port that mixes the gas-liquid switch, the algae liquid delivery port of reactor is connected with algae liquid harvesting system, and a sidewall of reactor is provided with Movable valve.
2. both culturing microalgae closed reactor according to claim 1 is characterized in that, also comprises magnetizing assembly, and described magnetizing assembly is serially connected on the pipeline between the input aperture of mixing gas-liquid switch and reactor.
3. both culturing microalgae closed reactor according to claim 2 is characterized in that, also comprises safety air vavle, and described safety air vavle is connected on the export pipeline that mixes the gas-liquid switch.
4. according to claim 1,2 or 3 described both culturing microalgae closed reactors, it is characterized in that, also comprise the carbonic acid gas supplementary device, described carbonic acid gas supplementary device is arranged on the bottom in the reactor.
CN 201110112849 2011-04-29 2011-04-29 Enclosed reactor for microalgae culture Expired - Fee Related CN102250758B (en)

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CN102250758B CN102250758B (en) 2013-06-05

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104719669A (en) * 2015-03-24 2015-06-24 临沂大学 Preparing and feeding method for chlamys farreri larva baits

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN201420080Y (en) * 2009-04-10 2010-03-10 张炳泉 Sealed microalgae industry photosynthesis cultivating device
CN201473543U (en) * 2009-07-03 2010-05-19 江西新大泽实业集团有限公司 Multifunctional pipeline microalgae cultivating device
CN201729830U (en) * 2010-08-11 2011-02-02 孙以川 Sealed pipeline culturing device for bait microalgae

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN201420080Y (en) * 2009-04-10 2010-03-10 张炳泉 Sealed microalgae industry photosynthesis cultivating device
CN201473543U (en) * 2009-07-03 2010-05-19 江西新大泽实业集团有限公司 Multifunctional pipeline microalgae cultivating device
CN201729830U (en) * 2010-08-11 2011-02-02 孙以川 Sealed pipeline culturing device for bait microalgae

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104719669A (en) * 2015-03-24 2015-06-24 临沂大学 Preparing and feeding method for chlamys farreri larva baits
CN104719669B (en) * 2015-03-24 2018-09-21 临沂大学 A kind of making of Chlamys farreri young bait and feeding method

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Address after: 518000 Wenhua Community, Huangbei Street, Luohu District, Shenzhen City, Guangdong Province, 27F, West Block, Wenhua Building, 1027 Shennan East Road

Patentee after: China Research Marine Biotechnology (Shenzhen) Co., Ltd.

Address before: 518000 Kexian Bioenergy Research Institute, No. 2 Kefa Road, Nanshan Science Park, Shenzhen City, Guangdong Province

Patentee before: Zhang Bingquan

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