CN102250753B - Device for drawing deoxyribonucleic acid (DNA) molecule into line shape and application thereof - Google Patents
Device for drawing deoxyribonucleic acid (DNA) molecule into line shape and application thereof Download PDFInfo
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- CN102250753B CN102250753B CN201110137516.7A CN201110137516A CN102250753B CN 102250753 B CN102250753 B CN 102250753B CN 201110137516 A CN201110137516 A CN 201110137516A CN 102250753 B CN102250753 B CN 102250753B
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Abstract
The invention discloses a device for drawing a deoxyribonucleic acid (DNA) molecule into a line shape and application thereof. The device disclosed by the invention comprises at least one pair of glass sheets for clamping the DNA molecule, wherein each pair of glass sheets comprise a first glass sheet for placing the DNA molecule and a second glass sheet for covering the first glass sheet; a space for accommodating the DNA molecule is formed between the first glass sheet and the second glass sheet; and the first glass sheet is a silylated glass sheet. The device also comprises a power mechanism for dragging the second glass sheet. The device used for preparing microscopic examination sample slices has the following advantages: 1. the speed is uniform and can be maintained at a set speed all along; 2. the direction is consistent, thereby guaranteeing that the drawn DNA is a straight line; 3. shaking is reduced to the minimum, so that the DNA fracture caused by shaking is maximally reduced; 4. the experiment consistency is guaranteed, multiple sample slices can be simultaneously drawn, and the consistency of the experiments on different sample slices is guaranteed; and 5. the device is simple to operate and agile to use, and the slice drawing speed can be randomly set.
Description
Technical field
The present invention relates to a kind of device and application thereof that DNA molecular is pulled into wire
Background technology
The way of traditional similar experiment be sheet glass as on an inclined-plane, allow the solution droplets that contains DNA trickle on inclined-plane, utilize stringy shearing force that DNA is spread upon on sheet glass.This way effect, efficiency are all poor, and the repeatability of experimental result is also poor.
Summary of the invention
The object of the present invention is to provide a kind of device that DNA molecular is pulled into wire.
Device provided by the invention comprises that at least one pair of clamps the sheet glass of described DNA molecular; Every pair of sheet glass includes: first sheet glass and of placing DNA molecular covers the second sheet glass of described the first sheet glass; Between described the first sheet glass and the second sheet glass, form the accommodation space of described DNA molecular; Described the first sheet glass is the sheet glass of silanization; Said apparatus also comprises the actuating unit of the second sheet glass described in tractive.
Above-mentioned actuating unit comprises motor and the leading screw connecting by shaft coupling, and described leading screw is connected with described the second sheet glass; Described motor drives described the second sheet glass to move axially along leading screw by described leading screw.
In another embodiment, said apparatus also comprises the buffer gear being arranged between described actuating unit and described the second sheet glass.
This is stated actuating unit and comprises motor and the leading screw connecting by shaft coupling, and described leading screw is connected with described the second sheet glass by described buffer gear.
Above-mentioned buffer gear can be a vibration damping sheet.
Above-mentioned buffer gear also can be interconnective line slideway and vibration damping sheet, and described line slideway is connected with described leading screw, and described vibration damping sheet is connected with described the second sheet glass.
Above-mentioned line slideway comprises 2 guide rails and 1 slide block, and the relative side of 2 guide rails has guide groove, and the two ends of slide block put in respectively in the guide groove of 2 guide rails, realizes and being slidably matched with the guide groove in 2 guide rails.
Preferably, the ball row of carrying use are set respectively at the two ends of slide block, slide block is listed in 2 guide grooves in guide rail and is moved by ball.
A nearlyer step says, above-mentioned vibration-damping sheet is elastic material, is preferably hardboard paper or plastic sheet.
Above-mentioned device also belongs to protection scope of the present invention in application DNA molecular being pulled in wire.
Above-mentioned DNA molecular is to be dissolved in the MES damping fluid of pH5.5.During application, the translational speed of the second sheet glass in above-mentioned device is that 1-1000 millimeter is per second.
Use this device to manufacture microscope inspection print with respect to the advantage of traditional-handwork film-making:
1, speed is even, can remain setting speed;
2, direction is consistent, guarantees that the DNA pulling out is straight line;
3, vibrations are down to minimum, have buffer gear (line slideway and/or vibration damping sheet), can reduce to greatest extent the DNA break that vibrations cause;
4, guarantee that experiment is consistent, can draw multiple prints simultaneously, guarantee the consistence between the different prints of experiment;
5, simple to operate, to use flexibly, pulling-on piece speed can arbitrarily be set.
Using the DNA molecular of microscopic examination wire, is from molecule rank, to check a kind of effective ways of DNA replication dna changing conditions.Continuous upgrading along with biological study content and laboratory facilities in recent science both at home and abroad and industrial circle, grows with each passing day to the demand of this type of experiment.This experimental installation has a good application prospect.
Accompanying drawing explanation
Fig. 1 is the structural representation of embodiment 1.
Fig. 2 is DNA metamorphosis photo in embodiment 2.
Embodiment
Below in conjunction with specific embodiment, the invention will be further described, but the present invention is not limited to following examples.
In following embodiment, if no special instructions, be ordinary method.
Embodiment 1, DNA molecular is pulled into the device of wire
As shown in Figure 1, the device that DNA molecular is pulled into wire provided by the invention, comprises actuating unit 1, buffer gear 2 and topworks 3.Wherein, topworks 3 is the sheet glass of a pair of clamping DNA molecular, is specifically as follows the cover glass 31 and the slide glass 32 that stack up and down, and wherein slide glass 32 was processed through silanization.Between cover glass 31 and slide glass 32, form the accommodation space of DNA molecular.Actuating unit 1 comprises motor 11 and the leading screw 12 connecting by shaft coupling, and motor 11 and leading screw 12 are coaxial.Buffer gear 2 comprises interconnective line slideway 21 and vibration damping sheet 22, line slideway 21 can adopt any type of guide rail in prior art, the line slideway 21 that the present embodiment adopts comprises 211,212 and 1 slide blocks 213 of 2 guide rails, the relative side of guide rail 211,212 has guide groove, the two ends of slide block 213 put in respectively in the guide groove of guide rail 211,212, realize and being slidably matched with the guide groove of guide rail 211,212.The ball row of carrying use can certainly be set respectively at the two ends of slide block 213, and slide block 213 is listed in by ball in the guide groove of guide rail 211,212 and moves.
Leading screw 12 is connected with vibration damping sheet 22 by slide block 213, and vibration damping sheet 22 is connected with cover glass 31.Vibration damping sheet 22 in the present embodiment is hardboard paper.
In the present embodiment, motor can be servomotor, and leading screw can be ball-screw.Device operational process of the present invention is as follows: start servomotor 11, thereby the horizontal axis that motor 11 drives ball-screw 12 rotations to realize ball-screw 12 moves, the also corresponding movement of line slideway 21 and vibration damping sheet 22, is moved thereby realize the relative slide glass 32 of cover glass 31.
In other embodiments, the actuating unit 1 in apparatus of the present invention can directly be connected with topworks 3, so buffer gear 2 can omit; Certainly, the buffer gear 2 in apparatus of the present invention also can omit a part, as removed line slideway 21 or removing vibration damping sheet 22; During actual production, line slideway 21 and leading screw 12 can be become one, thereby a guide rail needn't be set separately or can replace vibration damping sheet 22 in the projection of slide block 213 ends subsidiary " possessing suitable stiff and elasticity "; According to experiment, need ,Ye Kejiang topworks 3 to be set to 2 pairs of above topworkies.
The foregoing is only preferred embodiment of the present invention, in order to limit the present invention, within the spirit and principles in the present invention not all, any modification of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Embodiment 2, DNA molecular is pulled into wire concrete steps
DNA is dissolved in the MES damping fluid of pH 5.5, drops on slide glass 32, covered 31, starts motor 11, and leading screw 12 drives line slideway 21, hardboard paper 22 horizontal axis to move, thereby pulls cover glass 31, and DNA molecular is pulled into wire.In cover glass 31 drawing process, the photo of DNA change in shape is as shown in the A of Fig. 2, B, C and D.
The principle of the invention: DNA is dissolved in the MES damping fluid of pH 5.5, DNA end, by partially denaturing, exposes intramolecular hydrophobic group.There is hydrophobic interaction in hydrophobic group and silanized glass surface, makes DNA one end fixedly on slide glass.Along continuous straight runs slowly at the uniform velocity (1-1000 micron is per second) pulls cover glass, cover glass to pull liquid level to a lateral movement, and the surface tension while utilizing liquid level to pull between water and air phase, pulls into wire by DNA.Examine under a microscope the linear DNA molecule of glass sheet surface, can obtain intuitively changing relevant various biology conclusions to DNA molecular.
Claims (3)
1. DNA molecular is pulled into a device for wire, it is characterized in that: it comprises that at least one pair of clamps the sheet glass of described DNA molecular; Every pair of sheet glass includes: first sheet glass and of placing DNA molecular covers the second sheet glass of described the first sheet glass; Between described the first sheet glass and the second sheet glass, form the accommodation space of described DNA molecular; Described the first sheet glass is the sheet glass of silanization; Described device also comprises the actuating unit of the second sheet glass described in tractive;
Described device also comprises the buffer gear being arranged between described actuating unit and described the second sheet glass;
Described actuating unit comprises motor and the leading screw connecting by shaft coupling, and described leading screw is connected with described the second sheet glass by described buffer gear; Described motor drives described the second sheet glass to move axially along leading screw by described leading screw;
Described buffer gear is interconnective line slideway and vibration damping sheet, and described line slideway is connected with described leading screw, and described vibration damping sheet is connected with described the second sheet glass; Described vibration-damping sheet is hardboard paper or plastic sheet.
2. device claimed in claim 1 is pulling into DNA molecular the application in wire, and described DNA molecular is to be dissolved in the MES damping fluid of pH5.5.
3. application as claimed in claim 2, is characterized in that: the translational speed of the second sheet glass in device claimed in claim 1 is that 1-1000 micron is per second.
Priority Applications (1)
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CN201110137516.7A CN102250753B (en) | 2011-05-25 | 2011-05-25 | Device for drawing deoxyribonucleic acid (DNA) molecule into line shape and application thereof |
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CN201110137516.7A CN102250753B (en) | 2011-05-25 | 2011-05-25 | Device for drawing deoxyribonucleic acid (DNA) molecule into line shape and application thereof |
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CN102250753A CN102250753A (en) | 2011-11-23 |
CN102250753B true CN102250753B (en) | 2014-02-05 |
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Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101251866A (en) * | 2008-04-10 | 2008-08-27 | 中国科学院上海药物研究所 | Device and method for optimizing stretching direction in stretching molecular kinetics simulation |
CN201610428U (en) * | 2009-12-16 | 2010-10-20 | 重庆大学 | Cell stretching device with continuously-adjustable strain |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CA2743332A1 (en) * | 2001-09-13 | 2003-03-20 | Axela Inc. | Method and apparatus for assay based on light diffraction |
JP3683246B2 (en) * | 2002-10-02 | 2005-08-17 | 独立行政法人食品総合研究所 | Method for extending and fixing DNA |
KR101226957B1 (en) * | 2008-12-08 | 2013-02-07 | 한국전자통신연구원 | Disposable diagnostic kit |
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2011
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Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101251866A (en) * | 2008-04-10 | 2008-08-27 | 中国科学院上海药物研究所 | Device and method for optimizing stretching direction in stretching molecular kinetics simulation |
CN201610428U (en) * | 2009-12-16 | 2010-10-20 | 重庆大学 | Cell stretching device with continuously-adjustable strain |
Non-Patent Citations (1)
Title |
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JP特开2004-125601A 2004.04.22 |
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