CN102225175B - Traditional Chinese medicine dropping pill for treating headache and preparation method thereof - Google Patents
Traditional Chinese medicine dropping pill for treating headache and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a traditional Chinese preparation for treating headache and a preparation method thereof. The traditional Chinese preparation is prepared by the following steps: extracting and purifying six medicinal materials namely gastrodia elata, angelica, rhizome smilacis glabrae, radix polygoni multiflori preparata, divaricate saposhnikovia root and scorpion as raw materials according to a certain process and then adding pharmaceutically acceptable auxiliaries. The traditional Chinese preparation has the characteristics that preparation process is simple and practicable, and the traditional Chinese preparation is safe and has obvious pharmacological and pharmacodynamic effects.
Description
Technical field
The present invention relates to a kind of Traditional Chinese Medicine Dropping Pill of treating headache and preparation method thereof, belong to the pharmaceutical preparations technology field.
Background technology
At present, along with the raising of living standards of the people, the rhythm of life and work is accelerated, pressure strengthens, and the sickness rate of headache raises day by day.Headache belongs to motherland's medical science " wind syndrome of head " category, is one of neural commonly encountered diseases, clinically see so that headache due to internal injury more, during its outbreak pain unbearably, pain very promptly is prone to nausea, vomits clear saliva or prolongs and facial muscle, contracture is numb, the course of disease is very long.Numerous at all times doctors are tame to think that the paathogenic factor of having a headache is unsuccessful with feelings will, six climate exopathogens are attacked, QI and blood is main against random, pathogenic wind resistance key, stagnation of blood stasis outward.Chinese medicine thinks, head be " confluence of all yang-channels ", " gas of sun all can be in the mountain peak clearly for the blood of the five internal organs elite, six internal organs ".Internal organs are safe and comfortable, and QI and blood is orderly and clearly, then the clear spirit of brain and the hardship of no headache.If feelings will is unsuccessful, disgruntled anxiety, the liver failing to maintain the normal flow of QI, strongly fragrant and fire-transformation, yang-energy is high moving, and QI and blood is disorderly contrary, and QI-blood circulation is obstructed and forms stagnation of QI and blood; Six climate exopathogens are attacked outward, and wherein " wind being the primary exopathogen ", it can press from both sides cold, folder heat or be mingled with damp invasion and attack human body; Top, last criminal mountain peak; Also can cause QI-blood circulation not smooth, gradually become stagnation of QI and blood, so pathogenic wind hinders key, stagnation of blood stasis is the pathological factor of migraine phase; Internal organs yang blood and qi functional disorder is the pathologic basis of migraine phase, and six climate exopathogens are attacked outward, disorder of emotion then is the most common risk factor.Modern medicine is divided into angioneurotic headache, cluster headache, tension headache etc. with headache, but fully clear and definite as yet to its cause of disease, maybe be relevant with factors such as heredity, endocrine, nervous, direct stimulation, sleep disorder.Therefore, Chinese medicine has good effect on to the headache treatment of diseases, can reach treating both the principal and secondary aspects of a disease, the absolute advantages that side effect is little.Between this, we have successively applied for two patents of invention and have obtained mandate that application number is respectively 02153456.X and 200410022070.3; In these two patent application documents, mainly be from preparation prescription, the protection of aspects such as dosage form preparation technology; The present invention carries out a large amount of experimentatioies on this basis; With further replenishment of process method for preparing, improving medicine stability and bioavailability is purpose, and creationary a kind of quick-acting Chinese medicine preparation of treating headache of succeeding in developing.
Drop pill be by medicine with after the solid matrix heating and melting becomes solution, suspension or emulsion, splash in the not miscible condensed fluid, owing to surface tension effects is shunk drop and is condensed into a kind of quick-acting dosage forms that ball gets.Because medicine is molecularity, colloidal state or the crystallization of micropowder shape in substrate; Be the high degree of dispersion state, and substrate is water miscible, then can increase or improve the solubility property of medicine; Accelerate the dissolution rate and the infiltration rate of medicine, thereby reach efficient, quick-acting effects.Drop pill is to develop novel solid dosage form rapidly in recent years, belongs to the quick-acting dosage forms of solid dispersion class, has advantages such as release is rapid, bioavailability is high, dosage is accurate, easy to operate.
Summary of the invention
The object of the present invention is to provide a kind of steady quality, curative effect is reliable, the Traditional Chinese Medicine Dropping Pill of the treatment headache that bioavailability is high.The drop pill that the present invention prepares is through corresponding pharmacodynamics test research, and the result shows this Chinese medicine dripping pills preparation stable curative effect, effective, reliable.
Technical scheme of the present invention is to be formed by following prepared:
(1) get and be ground into 270 parts in coarse grained Rhizoma Gastrodiae, 180 parts of Radix Angelicae Sinensis are soaked, and after supersound process, heating extraction filters, and merging filtrate also adds ethanol, leaves standstill the back centrifugalize, and centrifugal liquid reclaims ethanol and concentrating under reduced pressure becomes clear paste subsequent use; Medicinal residues add 360 parts of Rhizoma Smilacis Glabraes, 270 parts of Radix Polygoni Multiflori Preparatas, and windproof 180 parts, after the adding ethanol supersound process, heating and refluxing extraction filters, and merging filtrate reclaims ethanol, and concentrating under reduced pressure becomes clear paste subsequent use;
(2) get Scorpio and be ground into fine powder for 30 parts, the clear paste that makes with two places in above-mentioned (1) mixes, dry and be ground into dried cream powder after, add substrate and cosolvent, mix homogeneously is selected suitable condensing agent for use and is dripped a system condition, drips and processes drop pill, promptly gets.
Technical scheme of the present invention specifically is to be prepared from following steps:
(1) gets and be ground into 270 parts in coarse grained rhizoma Gastrodiae; 180 parts of Radix Angelicae Sinensis; Add the water logging bubble that 6-12 doubly measures, and behind 40-100HZ sonicated 15-45min, heating and extracting 2 times; Each 1h; Filter, merging filtrate also adds ethanol and makes and contain the alcohol amount and be 55%-75%, leaves standstill 2000r-4000r centrifugation behind the 24-48h; Centrifugate reclaims ethanol, and to be condensed into relative density in 60 ℃ of decompressions be that the clear cream of 1.10-1.25 is subsequent use; The dregs of a decoction add 360 parts of smilaxs; 270 parts of prepared fleece flower roots; Windproof 180 parts; Doubly measure the alcohol immersion of 60%-90% with 6-10, and in 40-80HZ sonicated 10-30min, heating and refluxing extraction 2 times; Each 1h; Filter, merging filtrate reclaims ethanol, and to be condensed into relative density in 60 ℃ of decompressions be that the clear cream of 1.10-1.25 is subsequent use;
(2) get Scorpio and be ground into fine powder for 30 parts, the clear paste that makes with two places in above-mentioned (1) mixes, dry and be ground into dried cream powder after; Add the substrate and the cosolvent that can be used for drop pill on the pharmaceutics, mix homogeneously is selected suitable condensing agent for use and is dripped the system condition; Drip and process drop pill, promptly get.
Final preferable preparation technique method is following in the technical scheme of the present invention:
(1) gets and be ground into 270 parts in coarse grained rhizoma Gastrodiae; 180 parts of Radix Angelicae Sinensis; The water logging bubble that adds 10 times of amounts, and behind 80HZ sonicated 30min, heating and extracting 2 times; Each 1h; Filter, merging filtrate also adds ethanol and makes that to contain the alcohol amount be 70%, leaves standstill 3000r centrifugation behind the 24h; Centrifugate reclaims ethanol, and to be condensed into relative density in 60 ℃ of decompressions be that 1.20 clear cream is subsequent use; The dregs of a decoction add 360 parts of smilaxs, 270 parts of prepared fleece flower roots, and windproof 180 parts, with the alcohol immersion of 8 times of amounts 75%; And behind 60HZ sonicated 30min, heating and refluxing extraction 2 times, each 1h; Filter, merging filtrate reclaims ethanol, and to be evaporated to relative density in 60 ℃ be that 1.20 clear cream is subsequent use;
(2) get Scorpio and be ground into fine powder for 30 parts, the clear paste that makes with two places in above-mentioned (1) mixes, dry and be ground into dried cream powder after; Add the substrate and the cosolvent that can be used for drop pill on the pharmaceutics, mix homogeneously is selected suitable condensing agent for use and is dripped the system condition; Drip and process drop pill, promptly get.
Substrate in the above-mentioned method for preparing drop pill is polyethylene glycols, and what cosolvent was selected for use is polyethylene glycols, and what propylene glycol or tween 80, condensing agent were selected for use is a kind of or mixture of liquid paraffin, dimethicone, vegetable oil.
Technical scheme of the present invention is in the middle of the research process of reality, and two the patent 02153456.X and 200410022070.3 than in first to file mainly have following creationary useful technique effect:
1. the extraction of medical material and purification research:
A. because the drug loading of drop pill is less with respect to other conventional oral formulations such as tablet, granule, capsule, therefore need effective ingredient be extracted purification as much as possible, highly concentrated.The dosage form of application number 02153456.X and 200410022070.3 protections is common oral formulations commonly used; Process using part medical material is beaten the mode that powder is used as medicine and combines with alcohol extraction, and be used as medicine volume and weight of medical material is all bigger, and the effective ingredient enrichment is not enough; And owing to there is more a large amount of medical material fecula in the preparation; The stripping of effective ingredient and utilize limitedly, so the Rhizoma Gastrodiae that preparation technology of the present invention will be wherein proposes alcohol extraction afterwards with Radix Angelicae Sinensis two flavor medical materials elder generation water, exactly in order to overcome above-mentioned defective.Owing to all contain the water soluble ingredient of mass efficient in Rhizoma Gastrodiae and the Radix Angelicae Sinensis in headache treating; Like gastrodine, gastrodia elata polysaccharide, ferulic acid, ligustilide etc.; Therefore, earlier it is carried out water and carry, select alcohol deposition method then for use; To remove impurity components such as chlorophyll, polysaccharide, concentrated active substance reaches the purpose of efficient medication.In addition; We also take into full account the extraction of the pure dissolubility effective ingredient of medical material, and medicinal residues are carried out alcohol extraction with three flavor medical materials such as Rhizoma Smilacis Glabraes, have both reduced operating procedure; Can extract the effective ingredient of various different physicochemical properties again as much as possible, also reach the effect that improves extraction efficiency simultaneously.
B. we chance on need are extracted medical material earlier through after the ultrasonic pretreatment in the middle of the research process of invention, and its extraction ratio of effective constituents significantly improves; Mainly be because the chemical constituent in the medical material that prescription is formed; Through cavitation effect of ultrasonic waves, vibration effect and heating effect, can make the destruction of crude drug cell, thereby quicken the release and the stripping of effective ingredient; To be a very big saving to production cost and production capacity like this, finally be of value to the popularization of industrialization.
2. pharmacodynamic study
Owing to content of the present invention is to be to improve on the basis of 02153456.X and 200410022070.3 at application number, so our high spot reviews and this two public technology comparison of project in pharmacodynamics test.According to application number is the content of the patent of invention file record of 02153456.X and 200410022070.3; Discovery is in the technical scheme of patent of invention file of 02153456.X at application number; The technical scheme the most approaching with technology of the present invention arranged; Promptly prescription is formed and the identical Chinese medicine preparation preparation technology of recipe quantity, in line with contrast experiment's principle parallel, reasonable, that practice thrift, so finally we to select application number be 02153456.X patent document technology as a comparison.We bring different must the disturbing of curative effect at the dosage form difference of forgoing under study for action; The two pharmacodynamics difference in the medicinal material extract purifying process is relatively only promptly all processed the mode that dried cream powder is mixed with the same concentrations administering medical liquids then according to extraction process scheme separately with the two respectively and is compared.We are mainly from analgesia, sedation in the test of pesticide effectiveness; Experiment aspects such as hemorheology and meninges microcirculation are studied; Reached science, effectively illustrated the remarkable curative effect of medication of content of the present invention, the useful technique effect of finally the present invention being described and being reached.
2.1 laboratory animal: the Wistar rat, Kunming kind white mice, the male and female dual-purpose, the cleaning level is provided by The Fourth Military Medical University's Experimental Animal Center.
2.2 positive control drug: flunarizine (flunarizine hydrochloride capsules), Xian-Janssen Pharmaceutical Ltd. produces;
XUESAITONG JIAONANG, Wenshan Qihua Co., Ltd., Yunnan Baiyao Pharmaceutical Group produces;
The Qiyeshen an sheet, Hubei Li Shizhen (1518-1593 A.D.) medicine Group Co.,Ltd produces;
Beam balls all, the Beijing TongrenTang Co., Ltd produces.
2.3 the preparation of experiment drug group:
(1) gets and be ground into coarse grained rhizoma Gastrodiae 270g; Radix Angelicae Sinensis 180g; The water logging bubble that adds 10 times of amounts, and behind 80HZ sonicated 30min, heating and extracting 2 times; Each 1h; Filter, merging filtrate also adds ethanol and makes that to contain the alcohol amount be 70%, leaves standstill 3000r centrifugation behind the 24h; Centrifugate reclaims ethanol, and to be condensed into relative density in 60 ℃ of decompressions be that 1.20 clear cream is subsequent use; The dregs of a decoction add smilax 360g, prepared fleece flower root 270g, and windproof 180g is with the alcohol immersion of 8 times of amounts 75%; And behind 60HZ sonicated 30min, heating and refluxing extraction 2 times, each 1h; Filter, merging filtrate reclaims ethanol, and to be evaporated to relative density in 60 ℃ be that 1.20 clear cream is subsequent use;
(2) get Scorpio 30g and be ground into fine powder, the clear paste that makes with two places in above-mentioned (1) mixes, dry and be ground into dried cream powder after, subsequent use.
B. the preparation of application number 02153456.X drug group: get Scorpio 30g, windproof 50g, Rhizoma Gastrodiae 150g and be ground into fine powder, subsequent use.Other gets windproof 130g, Rhizoma Gastrodiae 120g and Rhizoma Smilacis Glabrae 360g, Radix Polygoni Multiflori Preparata 270g, Radix Angelicae Sinensis 180g, is ground into coarse granule, with 70% alcohol reflux secondary; Each 1 hour, filter merging filtrate; Decompression recycling ethanol continues to be condensed into thick paste, gained thick paste and windproof, Scorpio, Rhizoma Gastrodiae fine powder mixing; Drying under reduced pressure is pulverized, and is subsequent use.
The dried cream powder that above-mentioned A, B are prepared gained face with before, be mixed with the same concentrations administering medical liquids with distilled water.
2.4 analgesic experiment
2.4.1 mice acetic acid is caused the effect of writhing response: 40 of mices; Be divided into 4 groups at random, be administered once every day, and gastric infusion is 5 days continuously; Behind last gastric infusion 1h; Lumbar injection 0.6% glacial acetic acid 0.2ml/, observe and write down the number of times of mouse writhing reaction in the 20min (abdominal part indent, stretching, extension hind leg, buttocks are raised), the result sees table 1.
The analgesic activity
of table 1 pair mice acetic acid twisting reaction
Annotate: compare with the normal saline group,
* *P＜0.001; Compare with application number 02153456.X drug group,
△P＜0.05,
△ △ △P＜0.001.
2.4.2 influence to mice electricity irritation whipping pain threshold: 40 of mices, be divided into 4 groups at random, be administered once every day; Gastric infusion is 5 days continuously, behind last gastric infusion 1h, an electrode is put on the mice foot; Another electrode stimulating afterbody, after the energising, the pain threshold when observing the mice whipping; And calculating the analgesia suppression ratio, the result sees table 2.
Analgesia suppression ratio=(administration group pain threshold-matched group pain threshold)/matched group pain threshold * 100%
The influence
of table 2 pair mice electricity irritation whipping pain threshold
Annotate: compare with the normal saline group,
*P＜0.05,
* *P＜0.001; Compare with application number 02153456.X drug group,
△P＜0.05.
2.4.3 the mice hot plate is stimulated the influence of licking the pain in foot threshold value: get female mice and place (55 ℃ of temperature) on the hot-plate instrument; Mice is from placing hot-plate instrument to the pain threshold of metapedes required time as this Mus occurring licking; Allly lick the metapedes time less than 5 seconds or greater than 30 seconds or leaper; Be regarded as defective animal, give it up.With 40 of qualified mices, be divided into 4 groups at random, gastric infusion, continuous three days, once a day, and after the last administration 1 hour, begin to measure the mice pain threshold, the result sees table 3.
Table 3 pair mice hot plate stimulates the influence
of licking the pain in foot threshold value
Annotate: compare with the normal saline group,
*P＜0.05,
*P＜0.01,
* *P＜0.001; Compare with application number 02153456.X drug group,
△ △P＜0.01.
Table 1,2,3 experimental result show that the analgesic effect of drug group of the present invention and application number 02153456.X drug group more all has significant difference with the normal saline matched group; Drug group of the present invention and application number 02153456.X drug group relatively all have significant difference (P＜0.05) at the mice acetic acid twisting with in the experiment of mice electricity irritation whipping pain threshold, and stimulating to lick in the experiment of pain in foot threshold value at the mice hot plate has more excellent analgesia trend.
2.5 blood system contrast experiment
2.5.1 to the thrombotic influence of rats in vitro
Get 40 Wistar rats, be divided into 4 groups at random by body weight, 10 every group, continuous irrigation stomach 5 days; Every day 1 time, 1h after the administration on the 5th with 3.5% pentobarbital sodium lumbar injection 1ml/100g anesthesia, separates RCCA and left external jugular vein then; With the tubule (long 10cm) of 2 internal diameter 1mm, be enclosed within on the polyethylene tube of internal diameter 2mm (long 8cm), put 4 trumpeter's art silk threads of a long 5cm in the polyethylene tube, (50u/ml) is full of the polyethylene tube chamber with heparin-saline solution; After an end of pipe inserts left external jugular vein, inject heparin 50u/kg from polyethylene tube, clamp tube wall; The other end of pipe is inserted RCCA, after operation is accomplished, open blood flow; Then blood returns left external jugular vein from the RCCA polyethylene tube of flowing through, open blood flow after 15 minutes in Herba Clinopodii; Take out silk thread rapidly and weigh, it heavily is wet weight of thrombus that gross weight deducts silk thread, and the result sees table 4.
The thrombotic influence of table 4 pair rats in vitro
Annotate: compare with the normal saline group,
*P＜0.01.
The experimental result of table 4 shows that the external antithrombotic of drug group of the present invention and application number 02153456.X drug group more all has significant difference (P＜0.01) with the normal saline matched group; Drug group of the present invention and application number 02153456.X drug group relatively, though in the thrombotic experiment of rats in vitro there was no significant difference, it has more excellent trend.
2.5.2 influence to rat platelet aggregation
Get 40 Wistar rats, be divided into 4 groups at random, 10 every group by body weight; Continuous irrigation stomach 5 days, every day 1 time, 1h after the administration on the 5th; 3.5% pentobarbital sodium lumbar injection 1ml/100g anesthesia, dorsal position fixing limbs and head, it is total to separate neck; Intubate is got blood 1.8ml, puts into " platelet aggregation instrument " special use silication and that added the 0.2ml3.8g/L sodium citrate in advance in vitro.Begin to survey its platelet aggregation degree, the adenosine diphosphate (ADP) double sodium salt is as derivant, and the rule of operation of pressing " QX-200 whole blood platelet aggregation instrument " records the platelet maximum agglutination rate of every part of blood sample, and the result sees table 5.
The influence of table 5 pair rat platelet aggregation
Annotate: compare with the normal saline group,
*P＜0.05,
* *P＜0.001.
The experimental result of table 5 shows that drug group of the present invention and application number 02153456.X drug group more all have significant difference to the influence of rat platelet aggregation with the normal saline matched group; Drug group of the present invention and application number 02153456.X drug group compare, though both there was no significant differences, it has more excellent trend.
2.5.3 influence to cold blood stasis rat hemorheology index
Get 40 Wistar rats, be divided into 4 groups at random, continuous irrigation stomach 7 days, every day 1 time by body weight; Behind last administration 1h, fixedly rat extremity and head are used 70% alcohol disinfecting respectively; Put ice bag, freezing 1.5h, rewarming 5min in 45 ℃ of warm water around the experimental rat; Conventional then the raising after 3 days, blood sampling is also surveyed hemorheology numerical value, and the result sees table 6 and 7.
The influence of table 6 pair rat WBV
The influence of table 7 pair hemorheology of rat index
Annotate: compare with the normal saline group,
*P＜0.05,
*P＜0.01,
* *P＜0.001; Compare with application number 02153456.X drug group,
△P＜0.05,
△ △P＜0.01.
Table 6 shows with 7 experimental result; The whole blood viscosity of drug group of the present invention and 02153456.X group in, during low shear rate with the comparison of normal saline matched group; Have significant difference, both compare simultaneously, and drug group of the present invention has better curative effect than the 02153456.X group under high shear rate situation; In addition, compare with application number 02153456.X drug group, drug group of the present invention also has significant difference aspect plasma viscosity, aspect packed cell volume, have more excellent trend.
Can know that by the 2.5.1-2.5.3 experiment medicine of the present invention is evident in efficacy aspect anticoagulant and thrombolytic, have better effect than the 02153456.X group; Various acute and chronic blood clotting phenomenons had good inhibitory action; Especially with strong points to blood stasis due to the cold coagulation, this is because the blood viscosity increase causes being prone to be deposited on fixed attention blood vessel wall with part headache morbidity, thereby blocks blood circulation; Cause brain or whole body blood supply insufficiency, and the mechanism and the treatment means that cause having a headache match.
2.5.4 influence to the normal mouse hypoxia endurance time
Get 40 mices, be divided into 4 groups at random by body weight, continuous irrigation stomach 5 days, after the last administration 60 minutes, is surveyed mice anoxia enduring life span at every day 1 time; Test is with 4 of wide mouthed bottles, and volume 200ml adds sodica calx 70g in every bottle, adds a ground bottle cap above, builds bottle cap and timing, and stopping with mouse breathing is index, writes down mice life span (min), and the result sees table 8.
The influence of table 8 pair normal mouse hypoxia-bearing capability
Annotate: compare with the normal saline group,
*P＜0.05,
*P＜0.01,
* *P＜0.001; Compare with application number 02153456.X drug group,
△P＜0.05,
△ △P＜0.01.
The experimental result of table 8 shows, drug group of the present invention and 02153456.X group be to the influence of normal mouse hypoxia endurance time, with the normal saline matched group relatively, time that all can the existence of significant prolongation normal mouse, and have significant difference; Both compare simultaneously, and drug group of the present invention is obviously organized mice life span significant prolongation than 02153456.X, and has significant difference.
2.6 sedation
2.6.1 to pentobarbital sodium above threshold dosage cause the influence of mouse sleep time
Select 40 of mices for use, be divided into 4 groups at random, gastric infusion, continuous three days, once a day, 1 hour began to test after the last administration; Mouse peritoneal is injected pentobarbital sodium solution (55mg/kg); With injection pentobarbital sodium solution at once to righting reflex loss be incubation period; Be sleep time righting reflex loss to recovery time, notes incubation period and sleep time, and the result sees table 9.
Table 9 pair pentobarbital sodium above threshold dosage causes the influence
of mouse sleep time
Annotate: compare with the normal saline group,
* *P＜0.001;
The experimental result of table 9 shows that drug group of the present invention and 02153456.X group compare with the normal saline matched group, does not all have tangible the prolong incubation period of mice sleep and the effect of the length of one's sleep.
2.6.2 the pentobarbital sodium sub-threshold dose is caused the influence of mice sleep rate
Select 48 of mices for use, be divided into 4 groups at random, gastric infusion; Continuous three days, once a day, after the last administration 1 hour; Mouse peritoneal is injected pentobarbital sodium solution (35mg/kg), observe and note behind the animal injection pentobarbital sodium solution in 15 minutes, the mice righting reflex loss reaches the Mus number more than 1 minute; Calculate the sleep rate, the result sees table 10.
Table 10 pair pentobarbital sodium sub-threshold dose causes the influence
of mice sleep rate
Annotate: compare with the normal saline group,
*P＜0.05.
Can know by table 10, drug group of the present invention and 02153456.X group and normal saline matched group relatively, to the influence of mice sleep rate there are no significant difference; Results suggest: drug group of the present invention can not be worked in coordination with the sedative-hypnotic effect of pentobarbital sodium.
Can know by the 2.5.4-2.6.2 experiment; Medicine of the present invention can obviously promote the hypoxia-bearing capability of laboratory animal; Do not influence the mental status and the activity of laboratory animal during the medication simultaneously, i.e. the good oxygen lack resistant function of medicine performance is not to realize through the activity that reduces animal, points out medicine of the present invention not have remarkable sedative-hypnotic effect; Be applicable to the crowd of duty, and whole curative effect is superior to the 02153456.X group.
2.7 to the microcirculatory influence of rat head blood vessel
Select 40 of rats for use, be divided into 4 groups at random, gastric infusion, continuous three days; Once a day, after the last administration 1 hour,, rat abdominal cavity is anaesthetized with 1.00% pentobarbital sodium solution 0.5ml/100g; After the anesthesia rat ventricumbent position is fixed, cut skin of head, expose parietal bone; Puncture with No. 4 pins prior to XIAONAO oblongata place, extract cerebrospinal fluid 0.2ml, to reduce intracranial pressure.Then rats with left bone central authorities are windowed with cranial drill, form 0.5cm diameter circular bone hole, with the hemostasis by compression of warm saline cotton balls, cut off cerebral dura mater at last, instillation is done to be interrupted with 37 ℃ of artificial cerebrospinal fluids in the pia mater encephali surface, in case dry.Fixedly rat head is falling to penetrating under the light source, observe with 40 power microscopes, and near the top end diameter of maximum blood vessel loop arteriole flow velocity, vein input tap footpath, tremulous pulse output tap footpath, the vein, the result sees table 11.
The table 11 pair microcirculatory influence of rat head blood vessel
Annotate: compare with the normal saline group,
*P＜0.05,
*P＜0.01,
* *P＜0.001; Compare with application number 02153456.X drug group,
△P＜0.05,
△ △P＜0.01.
The experimental result of table 11 shows; Drug group of the present invention and 02153456.X group are in the microcirculatory influence experiment of rat head; Influence to arteriole flow velocity, input tap footpath, output tap footpath and loop top diameter more all has significant difference with the normal saline matched group; Simultaneously both compare, and the influence of rat arteriole flow velocity is had significant difference, though to the influence no difference of science of statistics of input tap footpath, output tap footpath and loop top diameter, have the trend of microcirculation improvement preferably.This matches with headache part pathogeny and Therapeutic Principle.
Show according to above research contents; Technology contents of the present invention is on the basis of application number 02153456.X; And combine the new technique of modern Chinese medicine development and the Chinese medicine preparation of the treatment headache succeeded in developing, and it has steady quality, and bioavailability is high; Drug action obviously is superior to the distinguishing feature of application number 02153456.X, and this will bring great potential and development space for this clinical practice of inventing preparation.
The specific embodiment
Embodiment 1
(1) gets and be ground into coarse grained rhizoma Gastrodiae 270g; Radix Angelicae Sinensis 180g; The water logging bubble that adds 10 times of amounts, and behind 80HZ sonicated 30min, heating and extracting 2 times; Each 1h; Filter, merging filtrate also adds ethanol and makes that to contain the alcohol amount be 70%, leaves standstill 3000r centrifugation behind the 24h; Centrifugate reclaims ethanol, and to be condensed into relative density in 60 ℃ of decompressions be that 1.20 clear cream is subsequent use; The dregs of a decoction add smilax 360g, prepared fleece flower root 270g, and windproof 180g is with the alcohol immersion of 8 times of amounts 75%; And behind 60HZ sonicated 30min, heating and refluxing extraction 2 times, each 1h; Filter, merging filtrate reclaims ethanol, and to be evaporated to relative density in 60 ℃ be that 1.20 clear cream is subsequent use;
(2) get Scorpio 30g and be ground into fine powder, the clear paste that makes with two places in above-mentioned (1) mixes, and the dry dried cream powder that also is ground into is excessively behind 100 mesh sieves; By fine drug powder: Macrogol 4000: polyethylene glycol 6000=carry out proportioning at 4: 5: 6 behind the substrate heating and melting, adds fine drug powder and cosolvent propylene glycol; Behind the mix homogeneously, melting mixing liquid is moved on to dropping-pill machine system, dripping the system temperature is 80 ℃; The water dropper diameter is 2mm, and condensing agent is selected dimethicone, 0 ℃ of condensation temperature; Dripping speed is 30 droplets/minute, drips apart from being 8cm, takes out drop pill by drop pill machine exit, removes the surface condensation agent, and drying promptly gets.
Embodiment 2
(1) gets and be ground into coarse grained rhizoma Gastrodiae 270g; Radix Angelicae Sinensis 180g; The water logging bubble that adds 6 times of amounts, and behind 100HZ sonicated 15min, heating and extracting 2 times; Each 1h; Filter, merging filtrate also adds ethanol and makes that to contain the alcohol amount be 55%, leaves standstill 2000r centrifugation behind the 32h; Centrifugate reclaims ethanol, and to be condensed into relative density in 60 ℃ of decompressions be that 1.25 clear cream is subsequent use; The dregs of a decoction add smilax 360g, prepared fleece flower root 270g, and windproof 180g is with the alcohol immersion of 6 times of amounts 60%; And behind 80HZ sonicated 10min, heating and refluxing extraction 2 times, each 1h; Filter, merging filtrate reclaims ethanol, and to be evaporated to relative density in 60 ℃ be that 1.10 clear cream is subsequent use;
(2) get Scorpio 30g and be ground into fine powder, the clear paste that makes with two places in above-mentioned (1) mixes, and the dry dried cream powder that also is ground into is excessively behind 100 mesh sieves; By fine drug powder: Macrogol 4000: polyethylene glycol 6000=carry out proportioning at 3: 8: 4 behind the substrate heating and melting, adds fine drug powder and cosolvent tween 80; Behind the mix homogeneously, melting mixing liquid is moved on to dropping-pill machine system, dripping the system temperature is 70 ℃; The water dropper diameter is 2mm, and condensing agent is selected liquid paraffin, 0 ℃ of condensation temperature; Dripping speed is 20 droplets/minute, drips apart from being 8cm, takes out drop pill by drop pill machine exit, removes the surface condensation agent, and drying promptly gets.
Embodiment 3
(1) gets and be ground into coarse grained rhizoma Gastrodiae 270g; Radix Angelicae Sinensis 180g; The water logging bubble that adds 12 times of amounts, and behind 40HZ sonicated 45min, heating and extracting 2 times; Each 1h; Filter, merging filtrate also adds ethanol and makes that to contain the alcohol amount be 75%, leaves standstill 4000r centrifugation behind the 48h; Centrifugate reclaims ethanol, and to be condensed into relative density in 60 ℃ of decompressions be that 1.10 clear cream is subsequent use; The dregs of a decoction add smilax 360g, prepared fleece flower root 270g, and windproof 180g is with the alcohol immersion of 6 times of amounts 90%; And behind 40HZ sonicated 20min, heating and refluxing extraction 2 times, each 1h; Filter, merging filtrate reclaims ethanol, and to be evaporated to relative density in 60 ℃ be that 1.25 clear cream is subsequent use;
(2) get Scorpio 30g and be ground into fine powder, the clear paste that makes with two places in above-mentioned (1) mixes, and drying also is ground into dried cream powder; After crossing 100 mesh sieves, by fine drug powder: Macrogol 4000: polyethylene glycol 6000=carry out proportioning at 7: 4: 8, with heat preservation for standby use behind the substrate heating and melting; With fine drug powder and the heat of solution altogether of cosolvent Macrogol 4000, add in the substrate behind the mix homogeneously, melting mixing liquid is moved on to dropping-pill machine system; Dripping the system temperature is 85 ℃; The water dropper diameter is 2mm, and condensing agent is selected soybean oil, 0 ℃ of condensation temperature; Dripping speed is 30 droplets/minute, drips apart from being 8cm, takes out drop pill by drop pill machine exit, removes the surface condensation agent, and drying promptly gets.
Embodiment 4
(1) gets and be ground into coarse grained rhizoma Gastrodiae 270g; Radix Angelicae Sinensis 180g; The water logging bubble that adds 8 times of amounts, and behind 60HZ sonicated 30min, heating and extracting 2 times; Each 1h; Filter, merging filtrate also adds ethanol and makes that to contain the alcohol amount be 60%, leaves standstill 3000r centrifugation behind the 24h; Centrifugate reclaims ethanol, and to be condensed into relative density in 60 ℃ of decompressions be that 1.20 clear cream is subsequent use; The dregs of a decoction add smilax 360g, prepared fleece flower root 270g, and windproof 180g is with the alcohol immersion of 10 times of amounts 80%; And behind 60HZ sonicated 10min, heating and refluxing extraction 2 times, each 1h; Filter, merging filtrate reclaims ethanol, and to be evaporated to relative density in 60 ℃ be that 1.20 clear cream is subsequent use;
(2) get Scorpio 30g and be ground into fine powder, the clear paste that makes with two places in above-mentioned (1) mixes, and drying also is ground into dried cream powder; After crossing 100 mesh sieves, by fine drug powder: Macrogol 4000=carry out proportioning at 2: 5, with heat preservation for standby use behind the substrate heating and melting; With fine drug powder and the heat of solution altogether of cosolvent polyethylene glycol 6000, add in the substrate behind the mix homogeneously, melting mixing liquid is moved on to dropping-pill machine system; Dripping the system temperature is 75 ℃; The water dropper diameter is 2mm, and condensing agent is selected dimethicone: soybean oil=1: 1,0 ℃ of condensation temperature; Dripping speed is 25 droplets/minute, drips apart from being 8cm, takes out drop pill by drop pill machine exit, removes the surface condensation agent, and drying promptly gets.
Embodiment 5
(1) gets and be ground into coarse grained rhizoma Gastrodiae 270g; Radix Angelicae Sinensis 180g; The water logging bubble that adds 10 times of amounts, and behind 80HZ sonicated 30min, heating and extracting 2 times; Each 1h; Filter, merging filtrate also adds ethanol and makes that to contain the alcohol amount be 70%, leaves standstill 3000r centrifugation behind the 24h; Centrifugate reclaims ethanol, and to be condensed into relative density in 60 ℃ of decompressions be that 1.20 clear cream is subsequent use; The dregs of a decoction add smilax 360g, prepared fleece flower root 270g, and windproof 180g is with the alcohol immersion of 8 times of amounts 75%; And behind 60HZ sonicated 30min, heating and refluxing extraction 2 times, each 1h; Filter, merging filtrate reclaims ethanol, and to be evaporated to relative density in 60 ℃ be that 1.20 clear cream is subsequent use;
(2) get Scorpio 30g and be ground into fine powder, the clear paste that makes with two places in above-mentioned (1) mixes, and drying also is ground into dried cream powder; After crossing 100 mesh sieves, by fine drug powder: polyethylene glycol 6000=carry out proportioning at 1: 2, with heat preservation for standby use behind the substrate heating and melting; With fine drug powder and the heat of solution altogether of cosolvent Macrogol 2000, add in the substrate behind the mix homogeneously, melting mixing liquid is moved on to dropping-pill machine system; Dripping the system temperature is 80 ℃; The water dropper diameter is 2mm, and condensing agent is selected dimethicone: liquid paraffin=2: 1,0 ℃ of condensation temperature; Dripping speed is 40 droplets/minute, drips apart from being 8cm, takes out drop pill by drop pill machine exit, removes the surface condensation agent, and drying promptly gets.
Embodiment 6
(1) gets and be ground into coarse grained rhizoma Gastrodiae 270g; Radix Angelicae Sinensis 180g; The water logging bubble that adds 8 times of amounts, and behind 80HZ sonicated 30min, heating and extracting 2 times; Each 1h; Filter, merging filtrate also adds ethanol and makes that to contain the alcohol amount be 70%, leaves standstill 3000r centrifugation behind the 24h; Centrifugate reclaims ethanol, and to be condensed into relative density in 60 ℃ of decompressions be that 1.20 clear cream is subsequent use; The dregs of a decoction add smilax 360g, prepared fleece flower root 270g, and windproof 180g is with the alcohol immersion of 8 times of amounts 75%; And behind 60HZ sonicated 30min, heating and refluxing extraction 2 times, each 1h; Filter, merging filtrate reclaims ethanol, and to be evaporated to relative density in 60 ℃ be that 1.20 clear cream is subsequent use;
(2) get Scorpio 30g and be ground into fine powder, the clear paste that makes with two places in above-mentioned (1) mixes, and the dry dried cream powder that also is ground into is excessively behind 100 mesh sieves; By fine drug powder: Macrogol 2000=carry out proportioning at 1: 3 behind the substrate heating and melting, adds fine drug powder and cosolvent propylene glycol; Behind the mix homogeneously, melting mixing liquid is moved on to dropping-pill machine system, dripping the system temperature is 80 ℃; The water dropper diameter is 2mm, and condensing agent is selected dimethicone: liquid paraffin: soybean oil=1: 1: 1,0 ℃ of condensation temperature; Dripping speed is 25 droplets/minute, drips apart from being 8cm, takes out drop pill by drop pill machine exit, removes the surface condensation agent, and drying promptly gets.
Embodiment 7
(1) gets and be ground into coarse grained rhizoma Gastrodiae 270g; Radix Angelicae Sinensis 180g; The water logging bubble that adds 12 times of amounts, and behind 100HZ sonicated 30min, heating and extracting 2 times; Each 1h; Filter, merging filtrate also adds ethanol and makes that to contain the alcohol amount be 75%, leaves standstill 3000r centrifugation behind the 24h; Centrifugate reclaims ethanol, and to be condensed into relative density in 60 ℃ of decompressions be that 1.20 clear cream is subsequent use; The dregs of a decoction add smilax 360g, prepared fleece flower root 270g, and windproof 180g is with the alcohol immersion of 8 times of amounts 75%; And behind 60HZ sonicated 30min, heating and refluxing extraction 2 times, each 1h; Filter, merging filtrate reclaims ethanol, and to be evaporated to relative density in 60 ℃ be that 1.20 clear cream is subsequent use;
(2) get Scorpio 30g and be ground into fine powder, the clear paste that makes with two places in above-mentioned (1) mixes, and the dry dried cream powder that also is ground into is excessively behind 100 mesh sieves; By fine drug powder: Macrogol 2000=carry out proportioning at 1: 2 behind the substrate heating and melting, adds fine drug powder and cosolvent propylene glycol; Behind the mix homogeneously, melting mixing liquid is moved on to dropping-pill machine system, dripping the system temperature is 80 ℃; The water dropper diameter is 2mm, and condensing agent is selected dimethicone: liquid paraffin: soybean oil=2: 1: 1,0 ℃ of condensation temperature; Dripping speed is 30 droplets/minute, drips apart from being 8cm, takes out drop pill by drop pill machine exit, removes the surface condensation agent, and drying promptly gets.
Claims (7)
1. Traditional Chinese Medicine Dropping Pill of treating headache is characterized in that it is to be prepared from following method:
(1) gets and be ground into 270 parts in coarse grained rhizoma Gastrodiae; 180 parts of Radix Angelicae Sinensis; Add the water logging bubble that 6-12 doubly measures, and behind 40-100HZ sonicated 15-45min, heating and extracting 2 times; Each 1h; Filter, merging filtrate also adds ethanol and makes and contain the alcohol amount and be 55%-75%, leaves standstill 2000r-4000r centrifugation behind the 24-48h; After centrifugate reclaims ethanol, and to be condensed into relative density in 60 ℃ of decompressions be that the clear cream of 1.10-1.25 is subsequent use; The dregs of a decoction add 360 parts of smilaxs; 270 parts of prepared fleece flower roots; Windproof 180 parts; Doubly measure the alcohol immersion of 60%-90% with 6-10, and in 40-80HZ sonicated 10-30min, heating and refluxing extraction 2 times; Each 1h; Filter, merging filtrate reclaims ethanol, and to be condensed into relative density in 60 ℃ of decompressions be that the clear cream of 1.10-1.25 is subsequent use;
(2) get Scorpio and be ground into fine powder for 30 parts, the clear paste that makes with two places in above-mentioned (1) mixes, dry and be ground into dried cream powder after; Add the substrate and the cosolvent that can be used for drop pill on the pharmaceutics, mix homogeneously is selected suitable condensing agent for use and is dripped the system condition; Drip and process drop pill, promptly get.
2. a kind of Traditional Chinese Medicine Dropping Pill of treating headache as claimed in claim 1 is characterized in that it is to be prepared from following steps:
(1) gets and be ground into 270 parts in coarse grained rhizoma Gastrodiae; 180 parts of Radix Angelicae Sinensis; The water logging bubble that adds 10 times of amounts, and behind 80HZ sonicated 30min, heating and extracting 2 times; Each 1h; Filter, merging filtrate also adds ethanol and makes that to contain the alcohol amount be 70%, leaves standstill 3000r centrifugation behind the 24h; Centrifugate reclaims ethanol, and to be condensed into relative density in 60 ℃ of decompressions be that 1.20 clear cream is subsequent use; The dregs of a decoction add 360 parts of smilaxs, 270 parts of prepared fleece flower roots, and windproof 180 parts, with the alcohol immersion of 8 times of amounts 75%; And behind 60HZ sonicated 30min, heating and refluxing extraction 2 times, each 1h; Filter, merging filtrate reclaims ethanol, and to be evaporated to relative density in 60 ℃ be that 1.20 clear cream is subsequent use;
(2) get Scorpio and be ground into fine powder for 30 parts, the clear paste that makes with two places in above-mentioned (1) mixes, dry and be ground into dried cream powder after; Add the substrate and the cosolvent that can be used for drop pill on the pharmaceutics, mix homogeneously is selected suitable condensing agent for use and is dripped the system condition; Drip and process drop pill, promptly get.
3. a kind of preparation technology who treats the Traditional Chinese Medicine Dropping Pill of headache as claimed in claim 1 is characterized in that it is to be prepared from following steps:
(1) gets and be ground into 270 parts in coarse grained rhizoma Gastrodiae; 180 parts of Radix Angelicae Sinensis; Add the water logging bubble that 6-12 doubly measures, and behind 40-100HZ sonicated 15-45min, heating and extracting 2 times; Each 1h; Filter, merging filtrate also adds ethanol and makes and contain the alcohol amount and be 55%-75%, leaves standstill 2000r-4000r centrifugation behind the 24-48h; After centrifugate reclaims ethanol, and to be condensed into relative density in 60 ℃ of decompressions be that the clear cream of 1.10-1.25 is subsequent use; The dregs of a decoction add 360 parts of smilaxs; 270 parts of prepared fleece flower roots; Windproof 180 parts; Doubly measure the alcohol immersion of 60%-90% with 6-10, and in 40-80HZ sonicated 10-30min, heating and refluxing extraction 2 times; Each 1h; Filter, merging filtrate reclaims ethanol, and to be condensed into relative density in 60 ℃ of decompressions be that the clear cream of 1.10-1.25 is subsequent use;
(2) get Scorpio and be ground into fine powder for 30 parts, the clear paste that makes with two places in above-mentioned (1) mixes, dry and be ground into dried cream powder after; Add the substrate and the cosolvent that can be used for drop pill on the pharmaceutics, mix homogeneously is selected suitable condensing agent for use and is dripped the system condition; Drip and process drop pill, promptly get.
4. a kind of preparation technology who treats the Traditional Chinese Medicine Dropping Pill of headache as claimed in claim 3 is characterized in that it is to be prepared from following steps:
(1) gets and be ground into 270 parts in coarse grained rhizoma Gastrodiae; 180 parts of Radix Angelicae Sinensis; The water logging bubble that adds 10 times of amounts, and behind 80HZ sonicated 30min, heating and extracting 2 times; Each 1h; Filter, merging filtrate also adds ethanol and makes that to contain the alcohol amount be 70%, leaves standstill 3000r centrifugation behind the 24h; Centrifugate reclaims ethanol, and to be condensed into relative density in 60 ℃ of decompressions be that 1.20 clear cream is subsequent use; The dregs of a decoction add 360 parts of smilaxs, 270 parts of prepared fleece flower roots, and windproof 180 parts, with the alcohol immersion of 8 times of amounts 75%; And behind 60HZ sonicated 30min, heating and refluxing extraction 2 times, each 1h; Filter, merging filtrate reclaims ethanol, and to be evaporated to relative density in 60 ℃ be that 1.20 clear cream is subsequent use;
(2) get Scorpio and be ground into fine powder for 30 parts, the clear paste that makes with two places in above-mentioned (1) mixes, dry and be ground into dried cream powder after; Add the substrate and the cosolvent that can be used for drop pill on the pharmaceutics, mix homogeneously is selected suitable condensing agent for use and is dripped the system condition; Drip and process drop pill, promptly get.
5. the substrate of the Traditional Chinese Medicine Dropping Pill of treatment headache according to claim 1 or claim 2, what it is characterized in that being selected for use is polyethylene glycols.
6. the cosolvent of the Traditional Chinese Medicine Dropping Pill of treatment headache according to claim 1 or claim 2, what it is characterized in that being selected for use is polyethylene glycols, propylene glycol or tween 80.
7. the condensing agent of the Traditional Chinese Medicine Dropping Pill of treatment headache according to claim 1 or claim 2, what it is characterized in that being selected for use is one or several the mixture in liquid paraffin, dimethicone, the vegetable oil.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1201781C (en) * | 2002-11-29 | 2005-05-18 | 咸阳步长制药有限公司 | Medicine compositions for treating head-ache, and its prepn. method |
| CN100358492C (en) * | 2004-03-17 | 2008-01-02 | 咸阳步长医药科技发展有限公司 | Preparation of Chinese traditional medicine for curing headache and preparation method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1201781C (en) * | 2002-11-29 | 2005-05-18 | 咸阳步长制药有限公司 | Medicine compositions for treating head-ache, and its prepn. method |
| CN100358492C (en) * | 2004-03-17 | 2008-01-02 | 咸阳步长医药科技发展有限公司 | Preparation of Chinese traditional medicine for curing headache and preparation method |
Non-Patent Citations (1)
| Title |
|---|
| 戴春禧.芎麻散合复方丹参滴丸治疗偏头痛40例.《中国中医药科技》.2007,第14卷(第2期),134. * |
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