CN102217616A - Fluopicolpkd-containing bactericidal composition - Google Patents
Fluopicolpkd-containing bactericidal composition Download PDFInfo
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Abstract
The invention discloses an agricultural bactericidal composition for prevention and treatment of downy mildew and eqpidemic diseases. The bactericidal composition comprises two active components of a first active component and a second active component, wherein the first active component is fluopicolpkd (having a structural formula is shown in the patent specification), and the second active component comprises one or more of propineb, cymoxanil, dimethomorph, flumorph, chlorothalonil, metalaxyl, fluazinam, fluopicolide and benthiavalicarb-isopropyl. A weight ratio of the first active component to the second active component is in a range of (50: 1) to (1: 50). The bactericidal composition disclosed by the invention has the advantages of good bactericidal effects, low pharmacy cost, safety on crops, and good effects of prevention and treatment of downy mildew and eqpidemic diseases of melons, fruit trees and vegetables.
Description
Technical field
The invention belongs to the composition pesticide field, be specifically related to a kind of bactericidal composition that is used to prevent and treat downy mildew and eqpidemic disease.
Background technology
Downy mildew and eqpidemic disease are the common frdquently encountered disease evils of crops such as melon, fruit tree, vegetables, have a strong impact on the yield and quality of product, usually become destructive disease.
Downy mildew is by the microbial plant disease of the downy mildew in the fungi.Downy mildew all can take place from seedling to each stage of results, is injured heavier to become strain.The blade of mainly causing harm is developed to upper leaf by base portion.Their early stage forms light yellow subcircular to the polygonal scab on the blade face, blade back produced the mould layer of white shape when there was dampness in the air, can spread to the blade face sometimes.The later stage scab is in flakes withered, is yellowish-brown, the withered and yellow death of whole siphonal lobes when serious.Germ, also can survive the winter on invalid body by egg spore at seed or cause harm on the vegetables autumn and winter and survive the winter with mycelia.Mainly by air-flow, water, farming and insect transmission.Germ spore germination temperature is 6～10 ℃ and suitable infects 15～17 ℃ of temperature, and field planting is overstocked, too early, excessive, the morbidities easily such as soil moisture big, impeded drainage of watering after the field planting.Spring is early summer or the easiest generation of continuous rainy weather in autumn not, can cause 20%～40% production loss when disease is serious.
Eqpidemic disease mainly is meant by the fungus-caused class disease of Phytophthora (Phytophthora), mainly cause plant flowers, really, the quick necrosis of leaf portion tissue and rotting.High humidity is to influence the principal element that disease takes place and propagates.Wherein capsicum epidemic disease, melon eqpidemic disease and potato late blight are important diseases.
The medicament that is used to prevent and treat downy mildew and eqpidemic disease at present mainly contains Propineb, white urea cyanogen, dimethomorph, flumorph, tpn, metalaxyl, fluazinam, fluorine Boscalid, benzene metsulfovax.
Propineb (propineb), chemical name: acrylic bisdithiocarbamic zinc.Propineb is the protective fungicide of long, wide spectrum of a kind of quick-acting, longevity of residure; downy mildew of crops such as vegetables, tobacco, lupulus and morning, the late blight of tomato and potato all there are good protective action, and the germ disease of powdery mildew, rust and Botrytis is also had certain inhibitory action.
Frost urea cyanogen (cymoxanil), chemical name: 1-(2-cyano group-2-methoxyl group imido grpup)-3-ethyl carbamide.Frost urea cyanogen is a kind of efficient, low toxicity bactericide, and Peronosporales fungi such as epidemic disease frost genus, Peronospora, single shaft frost are belonged to effectively.Frost urea cyanogen and other protective fungicides are used with extensively, are used in brassicaceous vegetable such as cucumber, grape, tomato, lichee, huge dish and tobacco etc.
Dimethomorph (dimethomorph), chemical name: (E, Z)-4-[3-(4-chlorphenyl) 3-(3, the 4-Dimethoxyphenyl) acryloyl] morpholine.It is the single-minded Oomycete fungi bactericide that kills, its effect characteristics are to destroy the formation of cell envelope, all there is effect in each stage to oomycetes history of life, and particularly responsive in the formation stage of sporangiophore and egg spore, (＜0.25 μ g/ml) promptly is suppressed under extremely low concentration.There is not cross resistance with the phenylamide medicament.Be widely used in vegetables downy mildew, eqpidemic disease, seedling stage damping off, black shank etc. by the fungus-caused disease control of Mastigomycotina Oomycete, the tool systemic activity.
Flumorph (flumorph), chemical name: 4-[3-(3, the 4-Dimethoxyphenyl)-3-(4-fluorophenyl) acryloyl] morpholine.Flumorph belongs to morpholine class disinfectant use in agriculture.Effective especially to Peronospora, epidemic disease mycin germ.To the Oomycete on grape, potato and the tomato, especially Peronosporaceae and Phytophthora bacterium have bactericidal activity preferably.
Tpn (chlorothalonil), chemical name: 2,4,5,6-tetrachloro-1,3-dicyanobenzenes.Tpn is wide spectrum, protective fungicide.The mechanism of action is to have an effect with the glyceraldehyde 3-phosphate dehydrogenase among the fungal cell, ties mutually entirely with the protein that contains cysteine in this enzyme, thereby destroys this enzymic activity, fungal cell's metabolism is damaged and loses one's life power.Inhale conduction in tpn does not have, but after being sprayed onto on the plant corpus, good tackness can be arranged on body surface, be difficult for being fallen, so the drug effect phase is longer by rain drop erosion.
Metalaxyl (metalaxyl), chemical name: N-(2, the 6-xylyl)-N-(2-methoxy acetyl group)-DL-alanine methyl esters.Metalaxyl is a systemic fungicide, has protection and therapeutic action, can be absorbed by the root, stem and leaf of plant, and with moisture transportation in the plant corpus, and transfer to each organ of plant.Two-way conductive performance is arranged, lasting period 10-14 days, but soil treatment lasting period period of greater than two months.To Pseudoperonospora cubensis, the microbial various crop downy mildew of phytophthora root rot, phytophthora root rot, the millet downy mildew of gourd, fruit and vegetable class are effective.
Fluazinam (fluazinam), chemical name: 3-chloro-N-(3-chloro-5-trifluoromethyl-2-pyridine radicals)-α, α, α-three fluoro-2,6-dinitro-right-toluidines.This product belongs to 2, and the 6-dinitroanilines is a protective fungicide.Very effective to interlink spore genus, Botrytis, Phytophthora, Plasmopara, Sclerotinia bacterium, the Botrytis cinerea of anti-benzimidazole type and dicarboximide class biocide agent also there is good result, resistance of rainwater washing against, the lasting period is long.
Fluorine Boscalid (Fluopicolide), chemical name: 2,6-two chloro-N-[(3-chloro-5-trifluoromethyl-2-pyridine radicals) methyl] benzamide.Common Oomycete diseases such as downy mildew, eqpidemic disease, late blight, damping off are had outstanding preventive effect, in addition also rice blast, gray mold, powdery mildew etc. are had certain preventive effect.To crop and Environmental security, have good system's conductivity and stronger thin layer penetration power, each main form of pathogen all there is good inhibitory effect, can provide comprehensively and lasting protection for young leaves, stem, stem tuber, young fruit.Because medicament can be through the blade face fast Absorption, thus resistance of rainwater washing against, for vegetables diseases prevention in rainy season provides reliable guarantee.Be mainly used on all kinds of vegetables and the grape crop, as control cucumber downy mildew and potato late blight.
Benzene metsulfovax (benthiavalicarb-isopropyl), chemical name: { (s) one 1 one [(R) one 1 one (6 phenyl-monofluorides and thiazole one 2 one bases) ethylamino formoxyls], one 2 monomethyl propyl group } carbamic acid isopropyl ester.The benzene metsulfovax is the cell wall synthetic inhibitor.Be a kind of new type bactericide,, the Oomycete germ of various crop all there is good active, can control the downy mildew of late blight, grape and other crops of potato and tomato effectively with lower dosage (25～75g a.i./hm2).Test shows that also the benzene metsulfovax reaches all effective to the downy mildew of cucurbits bacterium of methoxy acrylic acid class tool resistance for the phytophthora infestans to acid amide fungicides tool resistance.
Above-mentionedly prevent and treat downy mildew and eqpidemic disease medicament because long-term use has produced pesticide resistance in various degree, control efficiency reduces, and it is increasing to use dose separately.A large amount of uses of medicament have simultaneously increased residue of pesticide.The harm food security, the weighting ring environment pollution.Therefore will be the development need of science, the rational use of medicines with the bactericide compounded of the different mechanisms of action.
Pyrrole fluorine bacterium ester (chemical name: 3-fluorine methoxyl group-2-[2-(3,5,6-trichloropyridine base-2-oxygen ylmethyl)-phenyl]-methyl acrylate), have structure shown in the following structural formula (I),
Be that the QoI compounds with independent intellectual property right is developed in national southern agricultural chemicals initiative base, Zhejiang, center, applied for national inventing patent (CN1907024), pyrrole fluorine bacterium ester is to be the new type bactericide of the class mechanism of action uniqueness that develops of guide with natural antibiotics strobilurin A, stop the synthetic of cell ATP by the electronics transmission that suppresses between cytochrome b and the cl, thereby suppress the germ respiration.Fungicidal spectrum is wide, bactericidal activity is high, have protection and therapeutic action, and the disease that nearly all Ascomycetes, Basidiomycetes, Oomycete and imperfect fungus are caused all has good control efficiency, especially downy mildew and eqpidemic disease is had special efficacy.
Though pyrrole fluorine bacterium ester has above-mentioned advantage, has the use cost height, and use is easy to generate drug-fast defective separately.Therefore, pyrrole fluorine bacterium ester rationally is mixed with the bactericide of the different mechanisms of action, have significant synergies, improve control efficiency, reduced spraying times and dosage, reduce the effect of control cost, also help to avoid the generation and the suspension of pesticide resistance generation speed of germ resistance, can solve problems such as resistance in the agricultural chemicals list agent application process and cost.
Summary of the invention
The object of the present invention is to provide a kind of component reasonable, bactericidal effect is good, and drug cost is low, the agricultural bactericidal composition that is difficult for developing immunity to drugs.
For reaching goal of the invention the technical solution used in the present invention be:
A kind of agricultural bactericidal composition that is used to prevent and treat downy mildew and eqpidemic disease, contain two kinds of active components, wherein first kind of active component is pyrrole fluorine bacterium ester, second kind of active component is one or more combinations that are selected from Propineb, white urea cyanogen, dimethomorph, flumorph, tpn, metalaxyl, fluazinam, fluorine Boscalid or the benzene metsulfovax, and the weight ratio of first kind of active component and second kind of active component is 50: 1～1: 50.
The weight ratio of above-mentioned first kind of active component and second kind of active component is preferably 12: 1～and 1: 12.
Above-mentioned second kind of active component is preferably one or more combinations that are selected from dimethomorph, flumorph, tpn, metalaxyl or the benzene metsulfovax.
The present invention also provides a kind of disinfectant use in agriculture that is used to prevent and treat downy mildew and eqpidemic disease, contains the above-mentioned agricultural bactericidal composition based on active component 10%～80%, and all the other are that agricultural goes up acceptable carrier and auxiliary agent.Be preferably the above-mentioned agricultural bactericidal composition that contains based on active component 15%～70%.
According to method known in those skilled in the art, the present invention can be mixed with agricultural and go up any formulation that allows when containing the agriculture bactericide composition practical application of pyrrole fluorine bacterium ester, preparation formulation is water dispersible granules, suspending agent, wetting powder, microemulsion, aqueous emulsion preferably.
For water dispersible granules, those skilled in the art are familiar with the use corresponding adjuvants and finish the present invention very much.Dispersant is selected for use from polycarboxylate, lignosulfonates, alkylnaphthalene sulfonate; Wetting agent is selected from polyoxyethylene alcohol, alkyl sulfate, alkylsulfonate, naphthalene sulfonate; Disintegrant is selected from ammonium sulfate, urea, sucrose, glucose, aluminium chloride, citric acid, succinic acid, sodium bicarbonate; Binding agent is selected from diatomite, corn starch, PVA, carboxymethyl (ethyl) cellulose family; Filler is selected from diatomite, kaolin, white carbon, fine particle calcium carbonate, sepiolite, talcum powder, attapulgite, potter's clay.
To wetting powder, spendable auxiliary agent has: dispersant is selected from polycarboxylate, lignosulfonates, alkylnaphthalene sulfonate; Wetting agent is selected from alkyl sulfate, alkylsulfonate, naphthalene sulfonate; Filler is selected from ammonium sulfate, urea, sucrose, glucose, diatomite, kaolin, white carbon, fine particle calcium carbonate, talcum powder, attapulgite, potter's clay.
To suspending agent, spendable auxiliary agent has: dispersant is selected from polycarboxylate, lignosulfonates, alkylnaphthalene sulfonate, TERSPERSE 2425 (U.S. Huntsman Corporation produces, the alkyl naphthalene sulfonic acid salt); Alkyl phenol formaldehyde resin polyoxyethylene ether), farming breast 2201, this dish-60# (common name: sorbitan monostearate), emulsifier T-60 (common name: the sorbitan monostearate APEO), agricultural newborn 1601# (common name: phenethyl phenol polyoxyethylene poly-oxygen propylene aether), TERSPERSE 4894 (U.S. Huntsman Corporation product) emulsifier is selected from Nongru-700 # (common name:; Wetting agent is selected from the poly-basic ether formaldehyde condensation products sulphate of alkylphenol-polyethenoxy, alkylphenol-polyethenoxy base ether phosphate, phenethyl phenol polyethenoxy base ether phosphate, alkyl sulfate, alkylsulfonate, naphthalene sulfonate, TERSPERSE 2500 (U.S. Huntsman Corporation product); Thickener is selected from xanthans, polyvinyl alcohol, bentonite; Preservative is selected from formaldehyde, benzoic acid, Sodium Benzoate; Defoamer is silicone based defoamer; Antifreezing agent is selected from ethylene glycol, propane diols, glycerine, urea, inorganic salts such as sodium chloride.
To aqueous emulsion, spendable auxiliary agent has: emulsifier is selected from Nonyl pheno (EO=10) ether phosphate, triphen ethyl-phenol polyoxyethylene ether phosphate (agricultural newborn 600# phosphate), Nongru-700 #, agricultural newborn 2201#, Span-60#, emulsifier T-60, TX-10, agricultural newborn 1601#, agricultural newborn 600#, agricultural newborn 400#; Solvent is selected from dimethylbenzene, toluene, cyclohexanone, solvent naphtha (trade mark: S-150, S-180, S-200); Stabilizing agent is selected from triphenyl phosphite, epoxychloropropane, aceticanhydride; Antifreezing agent is selected from ethylene glycol, propane diols, glycerine, urea, inorganic salts such as sodium chloride; Thickener is selected from xanthans, polyvinyl alcohol, bentonite, aluminium-magnesium silicate; Preservative is selected from methyl alcohol, benzoic acid, Sodium Benzoate.
To microemulsion, spendable auxiliary agent has: emulsifier is selected from calcium dodecyl benzene sulfonate (agricultural newborn 500#), Nongru-700 #, agricultural newborn 2201#, this dish-60#, Tween-80-60#, TX-10, farming breast 1601, agricultural newborn 600#, agricultural newborn 400#; Assistant for emulsifying agent is selected from methyl alcohol, isopropyl alcohol, n-butanol, ethanol; Solvent is selected from cyclohexanone, N-Methyl pyrrolidone, dimethylbenzene, toluene, solvent naphtha (trade mark: S-150, S-180, S-200); Stabilizing agent is selected from triphenyl phosphite, epoxychloropropane.
Composition of the present invention can provide with the finished product preparation form, and promptly each material mixes in the composition; The composition of composition also can provide with single agent formulation, directly mixes in bucket (jar) before using.Concentrate of the present invention mixes the concentration that obtains required active substance usually with water.
Bactericidal composition of the present invention can be used for preventing and treating the important disease that is caused by oomycetes etc., is particularly suitable for preventing and treating downy mildew and the eqpidemic disease of melon, fruit tree and vegetables.It is farmland, orchard etc. that bactericidal composition of the present invention is used the place.
Bactericidal composition of the present invention also can with other compound with weeding, desinsection or bactericidal property particularly protective fungicide mix use, also can mix use with nematocide, growth regulator, nutrient for plants or soil conditioner.
The present invention also provides a kind of method of sterilization, before or after crop and/or fruit are susceptible, uses described bactericidal composition of the present invention to the place of crop and/or fruit and growth or storage.Can use by common method, as cast, injection, spraying, dusting, distribution.Amount of application of the present invention is with weather condition or crop state variation.The duration of protective effect is usually relevant with the content of individualized compound in the composition, and is relevant with extraneous factor, weather for example, but by using suitable formulation can slow down climatic influences.
Bactericidal composition of the present invention has the following advantages compared to existing technology:
The bactericidal composition component is reasonable; treatment adds protective effect, and bactericidal effect is good, reduces the medication number of times; drug cost is low; and its activity and bactericidal effect are not the simple superposition of each composition activity, but significant synergistic effect is arranged, and composition is made up of the active ingredient of different mechanism of action; action site increases; help overcoming and delay the drug-fast generation of germ, good to crop safety, meet the security requirement of pesticidal preparations.The present invention has the better prevention effect to melon, fruit tree, vegetables downy mildew and eqpidemic disease.
Embodiment
Come the present invention is further specified below in conjunction with specific embodiment, but do not limit the invention to these embodiments.One skilled in the art would recognize that the present invention contained in claims scope all alternatives, improvement project and the equivalents that may comprise.
One, biologicall test
Embodiment 1, pyrrole fluorine bacterium ester and Propineb, white urea cyanogen, dimethomorph, flumorph, tpn, metalaxyl, fluazinam, fluorine Boscalid, the composite Toxicity Determination of benzene metsulfovax to cucumber downy mildew
Subjects: cucumber downy mildew germ (Pseudoperonospora cubensis) is cultivated by the long-term live body of this laboratory.
Test method: with reference to " the agricultural industry criteria NY/T 1156.11-2008 of the People's Republic of China (PRC) ".Select the potted plant cucumber seedling of two leaf periods (plucking growing point) growing way unanimity, naturally dry behind the spray medicine, inoculate after the spray medicine is handled 24h, get the sick leaf of fresh cucumber downy mildew, dip in writing brush and to get distilled water and wash disease leaf back sporangium, be made into sporangia suspension (2-3 * 10
5Individual/ml), with inoculation sprayer (pressure 0.1MPa) even spraying inoculation on cucumber seedling, postvaccinal examination material moves to phytotron, keep relative moisture 100%, keep 15-24 ℃ of temperature, relative moisture about 90% behind the 24h, 5 days " Invest, Then Investigate " incidences are pressed disease index and are calculated preventive effect %.By the linear regression analysis between the logarithm value of control efficiency and series concentration, obtain the EC of each medicament
50Value is calculated co-toxicity coefficient (CTC) according to the abundant method of Sun Yun again.
1) investigation stage division
0 grade: anosis;
1 grade: lesion area accounts for below 5% of one-piece blade area;
3 grades: lesion area accounts for 6%～10% of one-piece blade area;
5 grades: lesion area accounts for 11%～25% of one-piece blade area;
7 grades: lesion area accounts for 26%～50% of one-piece blade area;
9 grades: lesion area accounts for more than 50% of one-piece blade area.
2) computational methods:
According to survey data, calculate disease index and control efficiency that each is handled.
The calculating of co-toxicity coefficient.Calculate each with disease index and handle control efficiency, carry out the data analysis statistics, obtain regression line EC with " DPS data handling system " 3.11 professional versions
50, correlation coefficient.EC that each that will measure according to Sun Yunpei (Y-P Sun) method handled
50Be converted into actual poison exponent (ATI); According to the proportioning of mixture, obtain theoretical poison exponent (TTI), calculate the co-toxicity coefficient (CTC) of mixture by following formula.
Theory is used the content (%) of toxicity index * B in using with of content (the %)+B of toxicity index * A in using with of toxicity index (TTI)=A with
If co-toxicity coefficient is greater than 120, showing has synergistic effect; If be starkly lower than 100 (below 80), be indicated as antagonism; Between 100～120, be indicated as summation action.
Table 1 toxicity test is table as a result
| Medicament | EC 50(μg/mL) | ATI | TTI | Co-toxicity coefficient CTC |
| Pyrrole fluorine bacterium ester (A) | 5.87 | 100 | ||
| Propineb (B) | 110.8 | 5.30 | ||
| Frost urea cyanogen (C) | 57.12 | 10.28 | ||
| Dimethomorph (D) | 28.07 | 20.91 | ||
| Flumorph (E) | 18.97 | 30.94 | ||
| Tpn (F) | 80.55 | 7.29 | ||
| Metalaxyl (G) | 115.68 | 5.07 | ||
| Fluazinam (H) | 20.44 | 28.72 | ||
| Fluorine Boscalid (I) | 12.23 | 48 | ||
| Benzene metsulfovax (J) | 7.22 | 81.30 | ||
| ?A+B(12∶1) | 5.32 | 110.34 | 92.72 | 119.01 |
| ?A+B(8∶1) | 5.52 | 106.34 | 89.48 | 118.85 |
| ?A+B(4∶1) | 5.01 | 117.17 | 81.06 | 144.54 |
| ?A+B(1∶1) | 5.59 | 105.01 | 52.65 | 199.45 |
| ?A+B(1∶4) | 22.21 | 26.43 | 24.24 | 109.03 |
| ?A+B(1∶8) | 25.11 | 23.38 | 15.82 | 147.75 |
| ?A+B(1∶12) | 29.33 | 20.01 | 12.58 | 159.03 |
| ?A+C(12∶1) | 5.21 | 112.67 | 93.10 | 121.02 |
| ?A+C(8∶1) | 5.53 | 106.15 | 90.03 | 117.90 |
| ?A+C(4∶1) | 5 | 117.40 | 82.06 | 143.07 |
| ?A+C(1∶1) | 5.59 | 105.01 | 55.14 | 190.44 |
| ?A+C(1∶4) | 17.99 | 32.63 | 28.22 | 115.61 |
| A+C(1∶8) | 24.66 | 23.80 | 20.25 | 117.56 |
| A+C(1∶12) | 27.15 | 21.62 | 17.18 | 125.84 |
| A+D(12∶1) | 5.09 | 115.32 | 93.92 | 122.79 |
| A+D(8∶1) | 5.11 | 114.87 | 91.21 | 125.94 |
| A+D(4∶1) | 4.98 | 117.87 | 84.18 | 140.02 |
| A+D(1∶1) | 5.56 | 105.58 | 60.46 | 174.63 |
| A+D(1∶4) | 10.23 | 57.38 | 36.73 | 156.23 |
| A+D(1∶8) | 14.86 | 39.50 | 29.70 | 133.01 |
| A+D(1∶12) | 17.55 | 33.45 | 26.99 | 123.91 |
| A+E(12∶1) | 4.99 | 117.64 | 94.69 | 124.24 |
| A+E(8∶1) | 4.32 | 135.88 | 92.33 | 147.17 |
| A+E(4∶1) | 4.78 | 122.80 | 86.19 | 142.48 |
| A+E(1∶1) | 5.23 | 112.24 | 65.47 | 171.43 |
| A+E(1∶4) | 8.99 | 65.29 | 44.75 | 145.90 |
| A+E(1∶8) | 10.21 | 57.49 | 38.61 | 148.89 |
| A+E(1∶12) | 13.48 | 43.55 | 36.25 | 120.12 |
| A+F(12∶1) | 5.26 | 111.60 | 92.87 | 120.17 |
| A+F(8∶1) | 4.35 | 134.94 | 89.70 | 150.44 |
| A+F(4∶1) | 4.71 | 124.63 | 81.46 | 153.00 |
| A+F(1∶1) | 5.67 | 103.53 | 53.65 | 192.99 |
| A+F(1∶4) | 12.99 | 45.19 | 25.83 | 174.93 |
| A+F(1∶8) | 17.04 | 34.45 | 17.59 | 195.83 |
| A+F(1∶12) | 20.99 | 27.97 | 14.42 | 193.92 |
| A+G(12∶1) | 5 | 117.40 | 92.70 | 126.65 |
| A+G(8∶1) | 4.44 | 132.21 | 89.45 | 147.80 |
| A+G(4∶1) | 4.76 | 123.32 | 81.01 | 152.22 |
| A+G(1∶1) | 5.93 | 98.99 | 52.54 | 188.42 |
| A+G(1∶4) | 13.02 | 45.08 | 24.06 | 187.41 |
| A+G(1∶8) | 19.66 | 29.86 | 15.62 | 191.18 |
| A+G(1∶12) | 27.01 | 21.73 | 12.37 | 175.66 |
| A+H(12∶1) | 5.22 | 112.45 | 94.52 | 118.98 |
| A+H(8∶1) | 4.53 | 129.58 | 92.08 | 140.73 |
| A+H(4∶1) | 4.87 | 120.53 | 85.74 | 140.57 |
| A+H(1∶1) | 5.86 | 100.17 | 64.36 | 155.64 |
| A+H(1∶4) | 11.92 | 49.24 | 42.98 | 114.59 |
| A+H(1∶8) | 12.37 | 47.45 | 36.64 | 129.51 |
| A+H(1∶12) | 15.47 | 37.94 | 34.20 | 110.94 |
| A+I(12∶1) | 4.23 | 138.77 | 96.00 | 144.55 |
| A+I(8∶1) | 4.25 | 138.12 | 94.22 | 146.59 |
| A+I(4∶1) | 4.8 | 122.29 | 89.60 | 136.49 |
| A+I(1∶1) | 5.41 | 108.50 | 74.00 | 146.63 |
| A+I(1∶4) | 7.56 | 77.65 | 58.40 | 132.95 |
| A+I(1∶8) | 9.06 | 64.79 | 53.78 | 120.48 |
| A+I(1∶12) | 10.86 | 54.05 | 52.00 | 103.95 |
| A+J(12∶1) | 4.32 | 135.88 | 98.56 | 137.86 |
| A+J(8∶1) | 4.25 | 138.12 | 97.92 | 141.05 |
| A+J(4∶1) | 4.09 | 143.52 | 96.26 | 149.10 |
| A+J(1∶1) | 5.38 | 109.11 | 90.65 | 120.36 |
| A+J(1∶4) | 5.56 | 105.58 | 85.04 | 124.15 |
| A+J(1∶8) | 5.06 | 116.01 | 83.38 | 139.14 |
| A+J(1∶12) | 5.84 | 100.51 | 82.74 | 121.48 |
Embodiment 2, pyrrole fluorine bacterium ester and Propineb, white urea cyanogen, dimethomorph, flumorph, tpn, metalaxyl, fluazinam, fluorine Boscalid, the composite Toxicity Determination of benzene metsulfovax to capsicum epidemic disease
Subjects: capsicum epidemic disease pathogen (Phytophthora capsici) is provided by biological institute of Zhejiang University, and has stronger pathogenic through evaluation.
Test method: with reference to " the agricultural industry criteria NY/T 1156.2-2006 of the People's Republic of China (PRC) " mycelial growth rate method.The capsicum epidemic disease pathogen is used the PDA medium culture, when treating that bacterium colony has just covered with culture dish, break into the bacterium piece at colony edge, the bacterium piece is moved to the toxic PDA medium central authorities that are mixed with in advance with transfer needle with the card punch of diameter 5mm, place then in 25 ℃ of incubators and cultivate, every processing repeats 4 times.Look CK colony growth situation, adopt the right-angled intersection method to measure and respectively handle colony diameter cm, obtain to proofread and correct and suppress percentage with slide calliper rule.The EC of each medicament is obtained in linear regression analysis between the probit value by inhibiting rate and the logarithm value of series concentration
50Value is calculated co-toxicity coefficient (CTC) according to the abundant method of Sun Yun again.
Drug effect is calculated: two diameters are surveyed in each bacterium colony right-angled intersection, represent the bacterium colony size with its mean.Obtain the colony growth inhibiting rate by following formula: colony growth inhibiting rate %=(the blank bacterium colony increases diameter-chemicals treatment bacterium colony and increases diameter) * 100/ blank bacterium colony increases diameter
Table 2 toxicity test result
| Medicament | EC 50(μg/mL) | ATI | TTI | Co-toxicity coefficient CTC |
| Pyrrole fluorine bacterium ester (A) | 0.31 | 100 | ||
| Propineb (B) | 1.07 | 28.97 | ||
| Frost urea cyanogen (C) | 0.35 | 88.57 | ||
| Dimethomorph (D) | 0.58 | 53.45 |
| Flumorph (E) | 0.54 | 57.41 | ||
| Tpn (F) | 0.30 | 103.33 | ||
| Metalaxyl (G) | 0.69 | 44.93 | ||
| Fluazinam (H) | 0.49 | 63.27 | ||
| Fluorine Boscalid (I) | 0.41 | 75.61 | ||
| Benzene metsulfovax (J) | 0.23 | 134.78 | ||
| ?A+B(12∶1) | 0.26 | 119.23 | 94.54 | ?126.12 |
| ?A+B(8∶1) | 0.28 | 110.71 | 92.11 | ?120.20 |
| ?A+B(4∶1) | 0.27 | 114.81 | 85.79 | ?133.83 |
| ?A+B(1∶1) | 0.32 | 96.88 | 64.49 | ?150.23 |
| ?A+B(1∶4) | 0.49 | 63.27 | 43.18 | ?146.53 |
| ?A+B(1∶8) | 0.62 | 50.00 | 36.86 | ?135.64 |
| ?A+B(1∶12) | 0.65 | 47.69 | 34.43 | ?138.50 |
| ?A+C(12∶1) | 0.24 | 129.17 | 99.12 | ?130.31 |
| ?A+C(8∶1) | 0.26 | 119.23 | 98.73 | ?120.76 |
| ?A+C(4∶1) | 0.21 | 147.62 | 97.71 | ?151.07 |
| ?A+C(1∶1) | 0.20 | 155.00 | 94.29 | ?164.40 |
| ?A+C(1∶4) | 0.24 | 129.17 | 90.86 | ?142.17 |
| ?A+C(1∶8) | 0.28 | 110.71 | 89.84 | ?123.23 |
| A+C(1∶12) | 0.26 | 119.23 | 89.45 | 133.29 |
| A+D(12∶1) | 0.28 | 110.71 | 96.42 | 114.83 |
| A+D(8∶1) | 0.29 | 106.90 | 94.83 | 112.73 |
| A+D(4∶1) | 0.21 | 147.62 | 90.69 | 162.77 |
| A+D(1∶1) | 0.29 | 106.90 | 76.73 | 139.32 |
| A+D(1∶4) | 0.27 | 114.81 | 62.76 | 182.94 |
| A+D(1∶8) | 0.38 | 81.58 | 58.62 | 139.16 |
| A+D(1∶12) | 0.41 | 75.61 | 57.03 | 132.58 |
| A+E(12∶1) | 0.26 | 119.23 | 96.72 | 123.27 |
| A+E(8∶1) | 0.27 | 114.81 | 95.27 | 120.52 |
| A+E(4∶1) | 0.22 | 140.91 | 91.48 | 154.03 |
| A+E(1∶1) | 0.30 | 103.33 | 78.71 | 131.29 |
| A+E(1∶4) | 0.28 | 110.71 | 65.93 | 167.93 |
| A+E(1∶8) | 0.36 | 86.11 | 62.14 | 138.57 |
| A+E(1∶12) | 0.38 | 81.58 | 60.69 | 134.43 |
| A+F(12∶1) | 0.22 | 140.91 | 100.26 | 140.55 |
| A+F(8∶1) | 0.24 | 129.17 | 100.37 | 128.69 |
| A+F(4∶1) | 0.19 | 163.16 | 100.67 | 162.08 |
| A+F(1∶1) | 0.21 | 147.62 | 101.67 | 145.20 |
| A+F(1∶4) | 0.24 | 129.17 | 102.66 | 125.81 |
| A+F(1∶8) | 0.22 | 140.91 | 102.96 | 136.86 |
| A+F(1∶12) | 0.23 | 134.78 | 103.07 | 130.76 |
| A+G(12∶1) | 0.24 | 129.17 | 95.76 | 134.88 |
| A+G(8∶1) | 0.25 | 124.00 | 93.88 | 132.08 |
| A+G(4∶1) | 0.22 | 140.91 | 88.99 | 158.35 |
| A+G(1∶1) | 0.31 | 100.00 | 72.47 | 138.00 |
| A+G(1∶4) | 0.38 | 81.58 | 55.94 | 145.82 |
| A+G(1∶8) | 0.41 | 75.61 | 51.05 | 148.11 |
| A+G(1∶12) | 0.45 | 68.89 | 49.17 | 140.11 |
| A+H(12∶1) | 0.25 | 124.00 | 97.17 | 127.61 |
| A+H(8∶1) | 0.26 | 119.23 | 95.92 | 124.30 |
| A+H(4∶1) | 0.23 | 134.78 | 92.65 | 145.47 |
| A+H(1∶1) | 0.25 | 124.00 | 81.64 | 151.90 |
| A+H(1∶4) | 0.34 | 91.18 | 70.62 | 129.12 |
| A+H(1∶8) | 0.35 | 88.57 | 67.35 | 131.51 |
| A+H(1∶12) | 0.37 | 83.78 | 66.10 | 126.76 |
| A+I(12∶1) | 0.20 | 155.00 | 98.12 | 157.96 |
| A+I(8∶1) | 0.22 | 140.91 | 97.29 | 144.83 |
| A+I(4∶1) | 0.21 | 147.62 | 95.12 | 155.19 |
| A+I(1∶1) | 0.22 | 140.91 | 87.81 | 160.48 |
| A+I(1∶4) | 0.28 | 110.71 | 80.49 | 137.55 |
| A+I(1∶8) | 0.30 | 103.33 | 78.32 | 131.94 |
| A+I(1∶12) | 0.31 | 100.00 | 77.49 | 129.06 |
| A+J(12∶1) | 0.23 | 134.78 | 102.68 | 131.27 |
| A+J(8∶1) | 0.22 | 140.91 | 103.86 | 135.67 |
| A+J(4∶1) | 0.20 | 155.00 | 106.96 | 144.92 |
| A+J(1∶1) | 0.15 | 206.67 | 117.39 | 176.05 |
| A+J(1∶4) | 0.17 | 182.35 | 127.82 | 142.66 |
| A+J(1∶8) | 0.18 | 172.22 | 130.92 | 131.55 |
| A+J(1∶12) | 0.16 | 193.75 | 132.10 | 146.66 |
Two, the configuration of preparation
Percentage composition is mass percent in all preparation proportionings.
1, water dispersible granules
Press formula rate, with former medicine and dispersant, wetting agent, binding agent etc. often the method for regulation water intaking dispersible granule promptly mix, the ultra micro comminution by gas stream, add in the comminutor that the certain specification screen cloth is housed and carry out granulation.And then drying, screening promptly get granular product.
Example of formulations 1:60% pyrrole fluorine bacterium ester Propineb water dispersible granules
Take by weighing 20% pyrrole fluorine bacterium ester, 40% Propineb, 3%TERSPERSE 2700,2% dispersing agent NNO (alkylnaphthalene sulfonate formaldehyde condensation products), 3% Nekal BX (nekal), 2%K-12 (lauryl sodium sulfate), 3% diatomite, 5% glucose, kaolin and add to 100%.
Example of formulations 2:40% pyrrole fluorine bacterium ester frost urea cyanogen water dispersible granules
Take by weighing 20% pyrrole fluorine bacterium ester, 20% white urea cyanogen, 3%TERSPERSE 2700,3% dispersing agent NNO, 2% Nekal BX, 1%K-12,1% carboxymethyl cellulose, 3% urea, diatomite and add to 100% mass parts.
Example of formulations 3:78% pyrrole fluorine bacterium ester dimethomorph water dispersion granula
Take by weighing 40% pyrrole fluorine bacterium ester, 38% dimethomorph, 4%TERSPERSE 2700,2% dispersing agent NNO, 2% Nekal BX, 1%K-12,3% corn starch, 3% sulphur ammonium, attapulgite and add to 100% mass parts.
Example of formulations 4:70% pyrrole fluorine bacterium ester flumorph water dispersible granules
Take by weighing 40% pyrrole fluorine bacterium ester, 30% flumorph, 4%TERSPERSE 2700,2% dispersing agent NNO, 2% Nekal BX, 1%K-12,3% corn starch, 3% sulphur ammonium, attapulgite and add to 100% mass parts.
Example of formulations 5:80% pyrrole fluorine bacterium ester chlorothalonil water dispersible granule
Take by weighing 40% pyrrole fluorine bacterium ester, 40% tpn, 4%TERSPERSE 2700,2% dispersing agent NNO, 2% Nekal BX, 1%K-12,3% corn starch, 3% sulphur ammonium, attapulgite and add to 100% mass parts.
Example of formulations 6:60% pyrrole fluorine bacterium ester metalaxyl water dispersible granules
Take by weighing 30% pyrrole fluorine bacterium ester, 30% metalaxyl, 3%TERSPERSE 2700,2% dispersing agent NNO (alkylnaphthalene sulfonate formaldehyde condensation products), 3% Nekal BX (nekal), 2%K-12 (lauryl sodium sulfate), 3% diatomite, 5% glucose, kaolin and add to 100%.
Example of formulations 7:50% pyrrole fluorine bacterium ester fluazinam water dispersible granules
Take by weighing 45% pyrrole fluorine bacterium ester, 5% fluazinam, 5% Negel, 7% sodium lignin sulfonate, 2%K-12 (lauryl sodium sulfate), 5% glucose, fine particle calcium carbonate and add to 100%.
Example of formulations 8:40% pyrrole fluorine bacterium ester fluorine Boscalid water dispersible granules
Take by weighing 5% pyrrole fluorine bacterium ester, 35% fluorine Boscalid, 5% Negel, 7% sodium lignin sulfonate, 2%K-12 (lauryl sodium sulfate), 5% urea, fine particle calcium carbonate and add to 100%.
Example of formulations 9:30% pyrrole fluorine bacterium ester benzene metsulfovax water dispersible granules
Take by weighing 25% pyrrole fluorine bacterium ester, 5% fluorine Boscalid, 5% polycarboxylate, 7% sodium lignin sulfonate, 2%K-12 (lauryl sodium sulfate), 5% ammonium sulfate, fine particle calcium carbonate and add to 100%.
2, suspending agent
Pressing formula rate, is medium with water, former medicine, dispersant, suspending agent and antifreezing agent etc. is joined in the batching still mix, and disperses 30min through ball mill or high speed shear, with making suspending agent behind the sand mill sand milling.
Example of formulations 10:40% pyrrole fluorine bacterium ester frost urea cyanogen suspending agent
Take by weighing 15% pyrrole fluorine bacterium ester, 25% white urea cyanogen, 3%TERSPERSE 4894,2%TERSPERSE 2500,3%TERSPERSE 2425,0.2% xanthans, 3% white carbon, 5% ethylene glycol, 0.3% benzoic acid, 0.5% silicone defoaming agent (trade name: s-29 Nanjing "four news" (new ideas applied chemistry product company produces), deionized water adds to 100% mass parts.
Example of formulations 11:40% pyrrole fluorine bacterium ester dimethomorph suspending agent
Take by weighing 15% pyrrole fluorine bacterium ester, 25% dimethomorph, 2%NNO, 2%TERSPERSE 2500,1% emulsifier T-60,3% Nongru-700 #, 0.1% xanthans, 3% white carbon, 5% propane diols, 0.5% formaldehyde, 0.5% silicone defoaming agent, deionized water adds to 100% mass parts.
Example of formulations 12:25% pyrrole fluorine bacterium ester flumorph suspending agent
Take by weighing 15% pyrrole fluorine bacterium ester, 10% flumorph, 3%TERSPERSE 4894,1%TERSPERSE 2500,1%TERSPERSE 2425,0.2% xanthans, 3% white carbon, 5% ethylene glycol, 0.3% benzoic acid, 0.5% silicone defoaming agent, deionized water adds to 100% mass parts.
Example of formulations 13:80% pyrrole fluorine bacterium ester tpn suspending agent
Take by weighing 40% pyrrole fluorine bacterium ester, 40% tpn, 3%TERSPERSE 4894,1%TERSPERSE 2500,1%TERSPERSE 2425,0.2% xanthans, 3% white carbon, 5% ethylene glycol, 0.3% benzoic acid, 0.5% silicone defoaming agent, deionized water adds to 100% mass parts.
Example of formulations 14:25% pyrrole fluorine bacterium ester fluazinam suspending agent
Take by weighing 12.5% pyrrole fluorine bacterium ester, 12.5% fluazinam, 3%TERSPERSE 4894,1%TERSPERSE 2500,1%TERSPERSE 2425,0.2% xanthans, 3% white carbon, 5% ethylene glycol, 0.3% benzoic acid, 0.5% silicone defoaming agent, deionized water adds to 100% mass parts.
Example of formulations 15:15% pyrrole fluorine bacterium ester fluorine Boscalid suspending agent
Take by weighing 10% pyrrole fluorine bacterium ester, 5% fluorine Boscalid, 10% polycarboxylate, 3% lauryl sulfate, 1% xanthans, 5% sodium chloride, deionized water adds to 100% mass parts.
Example of formulations 16:20% pyrrole fluorine bacterium ester benzene metsulfovax suspending agent
Take by weighing 10% pyrrole fluorine bacterium ester, 10% benzene metsulfovax, 10% polycarboxylate, 3% lauryl sulfate, 2% xanthans, 1% bentonite, 5% glycerine, deionized water add to 100% mass parts.
3, wetting powder
By the prescription requirement, mix after after the Roughpulverizer pulverizing, enter airslide disintegrating mill and fully pulverize the mixed wetting powder that gets.Example of formulations 17:80% pyrrole fluorine bacterium ester Wettable propineb powder
Take by weighing 30% pyrrole fluorine bacterium ester, 50% Propineb, 5% calcium lignosulfonate, 2% Nekal BX, 1%K-12,5% white carbon, kaolin and add to 100% mass parts.
Example of formulations 18:65% pyrrole fluorine bacterium ester frost urea cyanogen wetting powder
Take by weighing 60% pyrrole fluorine bacterium ester, 5% white urea cyanogen, 4% calcium lignosulfonate, 3%TERSPERSE 2700,2% Nekal BX, 2%K-12,5% white carbon, fine particle calcium carbonate and add to 100% mass parts.
Example of formulations 19:52% pyrrole fluorine bacterium ester dimethomorph wetting powder
Take by weighing 4% pyrrole fluorine bacterium ester, 48% dimethomorph, 4% calcium lignosulfonate, 4% dispersing agent NNO, 2% Nekal BX, 2%K-12,5% white carbon, attapulgite and add to 100% mass parts.
Example of formulations 20:60% pyrrole fluorine bacterium ester tpn wetting powder
Take by weighing 50% pyrrole fluorine bacterium ester, 10% tpn, 4% calcium lignosulfonate, 3%TERSPERSE 2700,2% Nekal BX, 2%K-12,5% white carbon, fine particle calcium carbonate and add to 100% mass parts.
Example of formulations 21:65% pyrrole fluorine bacterium ester metalaxyl wetting powder
Take by weighing 60% pyrrole fluorine bacterium ester, 5% metalaxyl, 4% calcium lignosulfonate, 3%TERSPERSE 2700,2% Nekal BX, 2%K-12,5% white carbon, fine particle calcium carbonate and add to 100% mass parts.
Example of formulations 22:80% pyrrole fluorine bacterium ester fluazinam wetting powder
Take by weighing 55% pyrrole fluorine bacterium ester, 25% fluazinam, 3% calcium lignosulfonate, 2% lauryl sodium sulfate, 10% white carbon, kaolin and add to 100% mass parts.
Example of formulations 23:80% pyrrole fluorine bacterium ester fluorine Boscalid wetting powder
Take by weighing 60% pyrrole fluorine bacterium ester, 20% fluorine Boscalid, 3% calcium lignosulfonate, 2% lauryl sodium sulfate, 10% white carbon, kaolin and add to 100% mass parts.
Example of formulations 24:80% pyrrole fluorine bacterium ester benzene metsulfovax wetting powder
Take by weighing 40% pyrrole fluorine bacterium ester, 40% benzene metsulfovax, 3% calcium lignosulfonate, 2% lauryl sodium sulfate, 10% white carbon, kaolin and add to 100% mass parts.
4, microemulsion
Example of formulations 25:15% pyrrole fluorine bacterium ester fluazinam microemulsion
Take by weighing 5% pyrrole fluorine bacterium ester, 10% fluazinam, 4%TX-10,5% Nongru-700 #, 6% agricultural newborn 500#, 4% agricultural newborn 1601#, 15% cyclohexanone, 5%N-methyl pyrrolidone, 5% n-butanol, 1% epoxychloropropane, through dissolving fully and mixing, deionized water adds to 100% mass parts, makes after the stirring.
Example of formulations 26:20% pyrrole fluorine bacterium ester flumorph microemulsion
Take by weighing 10% pyrrole fluorine bacterium ester, 10% flumorph, 5% agricultural newborn 400#, 4% agricultural newborn 500#, 6% agricultural newborn 1601#, 25% cyclohexanone, 5% dimethylbenzene, 5% isopropyl alcohol, 3% triphenyl phosphite, through dissolving fully and mixing, deionized water adds to 100% mass parts, makes after the stirring.
5, aqueous emulsion
Example of formulations 27:15% pyrrole fluorine bacterium ester fluazinam aqueous emulsion
Take by weighing 10% pyrrole fluorine bacterium ester, 5% fluazinam, 3% agricultural newborn 2201#, 1.5% Nonyl pheno (EO=10) ether phosphate, 2.5% triphen ethyl-phenol polyoxyethylene ether phosphate (agricultural newborn 600# acid phosphatide), 2%Span-60#, 1% epoxychloropropane, 5% ethylene glycol, 20% dimethylbenzene, 10% cyclohexanone, 0.3% xanthans, 0.5% benzoic acid, deionized water adds to 100% mass parts.Above-mentioned raw materials is through mixing, and high speed shear emulsification makes.
Example of formulations 28:10% pyrrole fluorine bacterium ester fluorine Boscalid aqueous emulsion
Take by weighing 5% pyrrole fluorine bacterium ester, 5% fluorine Boscalid, 3% agricultural newborn 2201#, 1.5% Nonyl pheno (EO=10) ether phosphate, 2.5% triphen ethyl-phenol polyoxyethylene ether phosphate (agricultural newborn 600# acid phosphatide), 2%Span-60#, 1% epoxychloropropane, 5% ethylene glycol, 20% dimethylbenzene, 10% cyclohexanone, 0.3% xanthans, 0.5% benzoic acid, deionized water adds to 100% mass parts.Above-mentioned raw materials is through mixing, and high speed shear emulsification makes.
Three, field control effectiveness test
Field Application Example 1: control cucumber downy mildew field control effectiveness test
Control cucumber downy mildew field control effectiveness test carries out field control effectiveness test with reference to medicine inspecting institute of the Ministry of Agriculture " pesticide field efficacy medicine test criterion () GB/T17980.26-2000 bactericidal agent for preventing and treating cucumber downy mildew ".The experimental field is positioned at academy of agricultural sciences, Shaoxing, Zhejiang Province city five and base.The sub-district of test medicine, contrast medicament and blank is handled and is adopted district's group arrangement at random, sub-district area 25m
2, 4 repetitions, investigation state of an illness radix before the dispenser, dispenser twice altogether, downy mildew is in their early stage during dispenser.7 days investigation result of the tests behind 7 days, 2 times medicines behind 1 medicine.4 samplings are at random adopted in every experimental plot, investigate 2 strains at every, investigate whole blades of every strain, record disease grade.
The disease grade scale is as follows:
0 grade: anosis;
1 grade: lesion area is put below 5% of whole blade face;
3 grades: lesion area is put 6%～10% of whole blade face;
5 grades: lesion area is put 11%～25% of whole blade face;
7 grades: lesion area is put 25%～50% of whole blade face;
9 grades: lesion area is put more than 50% of whole blade face.
The drug effect computational methods:
According to investigation result, calculate disease index and preventive effect according to following (1), (2) formula.Test data adopts Deng Kenshi duncan's new multiple range method (DMRT) to carry out statistical analysis.
In the formula: CK
0, CK
1Be respectively the forward and backward disease index of blank district dispenser; PT
0, PT
1Be respectively the forward and backward disease index of chemicals treatment district medicine
Table 3 control cucumber downy mildew field control effectiveness test is table as a result
Field Application Example 2: prevent and treat the downy mildew of garpe field control effectiveness test
Prevent and treat the downy mildew of garpe field control effectiveness test and carry out field control effectiveness test with reference to medicine inspecting institute of the Ministry of Agriculture " pesticide field efficacy medicine test criterion (two) GB/T17980.122-2004 bactericidal agent for preventing and treating downy mildew of garpe ".Test is carried out in vineyard, the Nan Wang village, suburb, Tai.The sub-district of test medicine, contrast medicament and blank is handled and is adopted district's group arrangement at random, 6 strains of area canopy chamber, sub-district, and 4 repetitions, grape variety is huge peak, and dispenser is 2 times altogether, and downy mildew is in their early stage during dispenser for the first time.Executed medicine for the second time at interval in 7 days.10 of every sub-district random inspections are taken out living newly climing then, and every tip is investigated whole blades from top to bottom, respectively by following standard recording morbidity progression.
The blade grade scale:
0 grade: no scab;
1 grade: lesion area accounts for below 5% of one-piece blade area;
3 grades: lesion area accounts for 6%～25% of one-piece blade area;
5 grades: lesion area accounts for 26%～50% of one-piece blade area;
7 grades: lesion area accounts for 51%～75% of one-piece blade area;
9 grades: lesion area accounts for more than 76% of one-piece blade area.
The drug effect computational methods are with field Application Example 1.
Table 4 is prevented and treated downy mildew of garpe field control effectiveness test table as a result
Field Application Example 3: control capsicum epidemic disease field control effectiveness test
Control capsicum epidemic disease field control effectiveness test is with reference to the regulation of medicine inspecting institute of the Ministry of Agriculture " GB/T 17980.32-2000 pesticide field efficacy medicine test criterion ()---bactericidal agent for preventing and treating capsicum epidemic disease ", and test is experimental field carried out in country southern agricultural chemicals initiative base, Zhejiang, center.The sub-district of test medicine, contrast medicament and blank is handled and is adopted district's group arrangement at random, sub-district area 15m
2, 4 repetitions, this test amounts to dispenser 1 time, and capsicum is in vegetative growth phase during dispenser, and capsicum epidemic disease is in their early stage.This test is investigated 2 times altogether, carries out the efficacy survey first time in 15 days behind the medicine, efficacy survey for the second time after 30 days.Every experimental plot random searching 5 points, every some investigation 5 strains, record diseased plant number, dead strain number or obviously withered plant number.
The disease grade scale is as follows:
0 grade: anosis;
1 grade: overground part only leaf, fruit has scab;
3 grades: terrestrial stem, branch have the brown rot spot;
5 grades: basal part of stem has the brown rot spot;
7 grades: terrestrial stem, branch and basal part of stem all have the brown rot spot, and the part branch is withered;
9 grades: complete stool is withered.
The drug effect computational methods:
According to investigation result, calculate disease index and preventive effect according to following (1), (2) formula.Test data adopts Deng Kenshi duncan's new multiple range method (DMRT) to carry out statistical analysis.
In the formula: CK
0, CK
1Be respectively the forward and backward disease index of blank district dispenser; PT
0, PT
1Be respectively the forward and backward disease index of chemicals treatment district medicine
Table 5 control capsicum epidemic disease field test results table
Field Application Example 4: prevent and treat the potato late blight field control effectiveness test
Prevent and treat the regulation of potato late blight field control effectiveness test with reference to medicine inspecting institute of the Ministry of Agriculture " GB/T 17980.34-2000 pesticide field efficacy medicine test criterion ()---bactericidal agent for preventing and treating potato late blight ", the sub-district of test medicine, contrast medicament and blank is handled and is adopted district's group arrangement at random, sub-district area 15m
2, 4 repetitions, this test amounts to dispenser twice, is in their early stage during dispenser for the first time, executes medicine for the second time after the week.This test is investigated 2 times altogether, and every experimental plot adopts diagonal to fix 5 samplings, gets 2 strains at every, looks into whole blades, record disease grade.
The drug effect computational methods are with field Application Example 1.
Disease grade scale following (is unit with the blade):
0 grade: anosis;
1 grade: lesion area accounts for below 5% of one-piece blade area;
3 grades: lesion area accounts for 6%～10% of one-piece blade area;
5 grades: lesion area accounts for 11%～20% of one-piece blade area;
7 grades: lesion area accounts for 21%～50% of one-piece blade area;
9 grades: lesion area accounts for more than 50% of one-piece blade area.
Table 6 is prevented and treated the potato late blight field control effectiveness test
In sum; a large amount of indoor biometricses and field control effectiveness test result show that present composition component is reasonable; treatment adds protective effect; bactericidal effect is good; reduce the medication number of times; drug cost is low; and its activity and bactericidal effect are not the simple superposition of each composition activity, but significant synergistic effect is arranged, and composition is made up of the active ingredient of different mechanism of action; action site increases; help overcoming and delay the drug-fast generation of germ, trial crops is not all had obvious harmful effect, the leaf look; growing ways etc. are all normal; safety is good, meets the security requirement of pesticidal preparations.The present invention has the better prevention effect to melon, fruit tree, vegetables downy mildew and eqpidemic disease.
Claims (8)
1. an agricultural bactericidal composition that is used to prevent and treat downy mildew and eqpidemic disease is characterized in that containing two kinds of active components, and wherein first kind of active component is pyrrole fluorine bacterium ester, has following structural formula (I),
Second kind of active component is one or more combinations that are selected from Propineb, white urea cyanogen, dimethomorph, flumorph, tpn, metalaxyl, fluazinam, fluorine Boscalid or the benzene metsulfovax, and the weight ratio of first kind of active component and second kind of active component is 50: 1～1: 50.
2. according to the described agricultural bactericidal composition that is used to prevent and treat downy mildew and eqpidemic disease of claim 1, the weight ratio that it is characterized in that first kind of active component and second kind of active component is 12: 1～1: 12.
3. according to the described agricultural bactericidal composition that is used to prevent and treat downy mildew and eqpidemic disease of claim 1, it is characterized in that described second active component is selected from one or more combinations in dimethomorph, flumorph, tpn, metalaxyl or the benzene metsulfovax.
4. a disinfectant use in agriculture that is used to prevent and treat downy mildew and eqpidemic disease is characterized in that containing based on one of active component 1%～80% claim 1 to 3 described agricultural bactericidal composition, and all the other are that agricultural goes up acceptable carrier and auxiliary agent.
5. according to the described a kind of disinfectant use in agriculture that is used to prevent and treat downy mildew and eqpidemic disease of claim 4, it is characterized in that containing based on one of active component 10%～80% claim 1 to 3 described agricultural bactericidal composition.
6. according to the described a kind of disinfectant use in agriculture that is used to prevent and treat downy mildew and eqpidemic disease of claim 5, it is characterized in that containing based on one of active component 15%～70% claim 1 to 3 described agricultural bactericidal composition.
7. according to the described a kind of disinfectant use in agriculture that is used to prevent and treat downy mildew and eqpidemic disease of claim 4, the formulation that it is characterized in that described bactericide is water dispersible granules, suspending agent, wetting powder, aqueous emulsion or microemulsion.
8. according to the described agricultural bactericidal composition of claim 1, it is characterized in that being used to preventing and treating downy mildew and the eqpidemic disease of melon, fruit tree and vegetables.
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| CN102415392A (en) * | 2011-12-23 | 2012-04-18 | 江阴苏利化学有限公司 | Germicide composition containing boscalid and cymoxanil |
| CN102812959A (en) * | 2012-09-05 | 2012-12-12 | 沈阳化工研究院（南通）化工科技发展有限公司 | Bactericidal suspending agent composition and application thereof |
| CN103081931A (en) * | 2013-01-17 | 2013-05-08 | 湖南农大海特农化有限公司 | Benthiavalicarb isopropyl/dimethomorph bactericidal composition |
| CN103098806A (en) * | 2013-01-27 | 2013-05-15 | 成都科利隆生化有限公司 | Benthiavalicarb isopropyl and fluazinam pesticide composition |
| CN104115853A (en) * | 2013-04-23 | 2014-10-29 | 陕西美邦农药有限公司 | Pesticide composition containing carbamate bactericide |
| CN104798786A (en) * | 2015-05-07 | 2015-07-29 | 深圳诺普信农化股份有限公司 | Sterilization composition containing benzovindiflupyr and application thereof |
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| CN102415392A (en) * | 2011-12-23 | 2012-04-18 | 江阴苏利化学有限公司 | Germicide composition containing boscalid and cymoxanil |
| CN102812959A (en) * | 2012-09-05 | 2012-12-12 | 沈阳化工研究院（南通）化工科技发展有限公司 | Bactericidal suspending agent composition and application thereof |
| CN103081931A (en) * | 2013-01-17 | 2013-05-08 | 湖南农大海特农化有限公司 | Benthiavalicarb isopropyl/dimethomorph bactericidal composition |
| CN103081931B (en) * | 2013-01-17 | 2016-02-17 | 湖南农大海特农化有限公司 | The bactericidal composition of benzene metsulfovax and dimethomorph |
| CN103098806A (en) * | 2013-01-27 | 2013-05-15 | 成都科利隆生化有限公司 | Benthiavalicarb isopropyl and fluazinam pesticide composition |
| CN104115853A (en) * | 2013-04-23 | 2014-10-29 | 陕西美邦农药有限公司 | Pesticide composition containing carbamate bactericide |
| CN104798786A (en) * | 2015-05-07 | 2015-07-29 | 深圳诺普信农化股份有限公司 | Sterilization composition containing benzovindiflupyr and application thereof |
| CN104798786B (en) * | 2015-05-07 | 2016-07-06 | 深圳诺普信农化股份有限公司 | A kind of bactericidal composition containing benzo alkene fluorine bacterium azoles and application thereof |
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| CN102217616B (en) | 2013-10-16 |
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