CN102217607B - Hymexazol nanoemulsion and formulation method - Google Patents
Hymexazol nanoemulsion and formulation method Download PDFInfo
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- CN102217607B CN102217607B CN 201110106177 CN201110106177A CN102217607B CN 102217607 B CN102217607 B CN 102217607B CN 201110106177 CN201110106177 CN 201110106177 CN 201110106177 A CN201110106177 A CN 201110106177A CN 102217607 B CN102217607 B CN 102217607B
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- hymexazol
- mould clever
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Abstract
The invention provides a hymexazol nanoemulsion capable of preventing and treating cotton verticillium wilt and a formulation method of the nanoemulsion. The hymexazol nanoemulsion comprises 2.0wt%-9.0wt% of hymexazol, 5.0wt%-20.0wt% of cyclohexanone, 3.0wt%-10.0wt% of antifreeze agent, 2.0wt%-5.0wt% of defoaming agent, 10.0wt%-20.0wt% of emulsifying agent and the balance of water. As the hymexazol nanoemulsion has inhibitory action on the growth of cotton verticillium wilt bacteria hypha, the micro sclerotium germination of cotton verticillium wilt bacteria, and favorable inhibitory action on the germination of cotton verticillium wilt bacteria spore, the hymexazol nanoemulsion can well inhibit the attach probability of cotton verticillium wilt. Adn the hymexazol nanoemulsion is simple in preparation, and available for large-scale production and application, and dramatically boosts the cotton output of China.
Description
Technical field
The present invention relates to a kind of mould clever nano-emulsion of evil and compound method thereof for the control cotton verticillium wilt.
Background technology
The Important Economic crop of cotton China, in recent years, cotton verticillium wilt increases the weight of year by year, serious contusion the cotton grower plant cotton enthusiasm.Particularly 1993,1995,1996,2002,2003,2004 etc. continuously in the North china great outburst, wherein 1993 is special outbreak year, onset area reaches more than 50% of cultivated area, loses then about 40,000 tons of gined cotton, causes grave danger to Cotton Production.Why this disease can cause harm so rampantly and be difficult to control, and its main cause is that its pathogen can be on the crop invalid body, in addition in the medium-term and long-term survival of soil for 10 years, and the bacterium amount can accumulate throughout the year.Verticillium wilt has become the main barrier factors that affects output of cotton and quality.Verticillium wilt belongs to soil biography, vascular bundle diseases, and cultivating disease-resistant variety is this sick main method of control, but because the factors such as high resistance or resisting verticillium upland cotton antigen shortage, the breeding for disease resistance effect is unsatisfactory.
Adopt modern genetic engineering breeding method the end of the nineties in last century, list the national " 863 " plan in, use pollen tube passage method, disease-resistant gene is imported cotton, breeding resistant variety, but do not have real resisting verticillium kind really up to the mark to use on producing so far.As seen, the resisting verticillium breeding process is slower, can't resolve to produce the upper task of top priority.In the agricultural measures, although but the especially generation of rice field-upland field rotation establishment verticillium wilt of crop rotation is subjected to the restriction of natural conditions or the impact of cropping system, and this technology is difficult to generally be promoted.The control of chemical control Chinese medicine does not also have the effective medicament of comparatively ideal control cotton verticillium wilt so far aborning.Biological control is effective measures of preventing and treating soil-borne disease, yet prevention practice for many years shows, only give birth to the biocontrol microorganisms that survey is screened and this traditional trilogy of Field Screening obtains by indoor flat plate face-off screening, greenhouse, how unstable its field efficacy is between year, greatly limited biological control from the laboratory stage true development to commercialization.
Summary of the invention
The invention provides a kind of mould clever nano-emulsion of evil and the compound method thereof that can prevent and treat cotton verticillium wilt.
The mould clever nano-emulsion of evil of the present invention comprises and dislikes mould clever 2.0wt%～9.0wt%, cyclohexanone 5.0wt%～20.0wt%, antifreeze 3.0wt%～10.0wt%, defoamer 2.0wt%～5.0wt%, emulsifier 10.0wt%～20.0wt%, water surplus.
Wherein, preferred, the mould clever content of described evil is 5.0wt%.
Wherein, described emulsifier is the Polyoxyalkylene ethers phosphoric acid ester.
Wherein, described emulsifier is the polyoxyethylene nonylphenol ether phosphate.
Wherein, described antifreeze is hexylene glycol.
Wherein, described defoamer is dimethicone.
The preparation method of the mould clever nano-emulsion of evil of the present invention comprises the steps:
(1) the hymexazol medicine is dissolved in the solvent cyclohexanone;
(2) solution and the emulsifier mix and blend of step (1) is abundant, form uniform oil phase;
(3) under stirring condition, water is joined in the oil phase of step (2), add simultaneously an amount of antifreeze and defoamer, freely disperse to obtain the mould clever nano-emulsion of evil of homogeneous phase transparent.
Wherein, described emulsifier is the Polyoxyalkylene ethers phosphoric acid ester.
Wherein, described emulsifier is the polyoxyethylene nonylphenol ether phosphate.
Wherein, described antifreeze is hexylene glycol.
Wherein, described defoamer is dimethicone.
Beneficial effect of the present invention is as follows:
Because the mould clever nano-emulsion of evil of the present invention has inhibitory action to the verticillium dahliae mycelial growth; Sprouting has inhibitory action to the verticillium dahliae Microsclerotia; Sprouting to the verticillium dahliae spore has good inhibitory action, therefore the mould clever nano-emulsion of evil of the present invention can suppress the incidence probability of cotton verticillium wilt well, and the mould clever nano-emulsion preparation of evil of the present invention is simple, can large batch of production and application, significantly improve the output of Cotton in China.
Embodiment
The compound method of the mould clever nano-emulsion of evil of the present invention is as follows:
Embodiment 1
The hymexazol medicine 0.2g of 95wt% is dissolved in the 0.4mL cyclohexanone solvent, and fully dissolving adds emulsifier polyoxyethylene nonylphenol ether phosphoesterase 30 .4mL; Be stirred into the transparent body, add afterwards antifreeze hexylene glycol 0.2mL and defoamer dimethicone 0.1mL, add simultaneously running water 2.5mL, stir into the transparent body, be and dislike mould clever nano-emulsion 1, this dislikes consisting of of mould clever nano-emulsion 1: dislike mould clever 5.0wt%, cyclohexanone 10.5wt%, antifreeze 5.0wt%, defoamer 2.6wt%, emulsifier 10.5wt%, water surplus.
Embodiment two:
95wt% hymexazol medicine 0.2g is dissolved in the 0.6mL cyclohexanone solvent, and fully dissolving adds emulsifier polyoxyethylene nonylphenol ether phosphoesterase 30 .4mL; Be stirred into the transparent body, add afterwards antifreeze hexylene glycol 0.2mL and defoamer dimethicone 0.1mL, add simultaneously running water 2.3mL, stir into the transparent body, be and dislike mould clever nano-emulsion 2, this dislikes consisting of of mould clever nano-emulsion 2: getting above-mentioned component density is 1, dislikes mould clever 5.0wt%, cyclohexanone 15.8wt%, antifreeze 5.0wt%, defoamer 2.6wt%, emulsifier 10.5wt%, water surplus.
Embodiment three
The hymexazol medicine 0.2g of 95wt% is dissolved in the 0.5mL cyclohexanone solvent, and fully dissolving adds emulsifier polyoxyethylene nonylphenol ether phosphate 1.5mL; Be stirred into the transparent body, add afterwards antifreeze hexylene glycol 0.6mL and defoamer dimethicone 0.3mL, add simultaneously running water 6.4mL, stir into the transparent body, be and dislike mould clever nano-emulsion 3, this dislikes consisting of of mould clever nano-emulsion 3: getting above-mentioned component density is 1, dislikes mould clever 2.0wt%, cyclohexanone 5.3wt%, antifreeze 6.3wt%, defoamer 3.2wt%, emulsifier 15.8wt%, water surplus.
Embodiment four
The hymexazol medicine 0.2g of 95wt% is dissolved in the 0.4mL cyclohexanone solvent, and fully dissolving adds emulsifier polyoxyethylene nonylphenol ether phosphoesterase 30 .4mL; Be stirred into the transparent body, add afterwards antifreeze hexylene glycol 0.2mL and defoamer dimethicone 0.1mL, add simultaneously running water 0.8mL, stir into the transparent body, be and dislike mould clever nano-emulsion 4, this dislikes consisting of of mould clever nano-emulsion 4: getting above-mentioned component density is 1, dislikes mould clever 9.0wt%, cyclohexanone 19.0wt%, antifreeze 9.5wt%, defoamer 4.8wt%, emulsifier 19.0wt%, water surplus.
Of the present invention above-mentioned three kinds dislike mould clever nano-emulsions 1,3,4 (because it is identical to dislike the content of mould spirit in the nano-emulsion 1 and 2, only selecting nano-emulsion 1 to be experimental subjects) to cotton verticillium wilt to prevent and treat experimental data as follows:
1, the mould clever nano-emulsion of evil of the present invention is to the inhibition test of verticillium dahliae mycelial growth.
Table 1 is disliked mould clever nano-emulsion to the inhibition of verticillium dahliae mycelial growth
From table 1, demonstrate, contrast with clear water (sterile water), dislike mould clever nano-emulsion various dose the verticillium dahliae mycelial growth is all had inhibitory action more than 60% for above-mentioned three kinds.Wherein nano-emulsion 1 is being used under 5 kinds of various dose, and it suppresses verticillium wilt pathogen mycelial growth effect all more than 65%, and nano-emulsion 4 inhibition when using five kinds of dosage is suitable with nano-emulsion 1.
2, the mould clever nano-emulsion of evil of the present invention is to the inhibition test of verticillium dahliae Microsclerotia sprouting
The impact that table 2 nano-emulsion is sprouted the verticillium dahliae Microsclerotia
From table 2, demonstrate, compare with the sterile water contrast, dislike mould clever nano-emulsion various dose for three kinds and the verticillium dahliae Microsclerotia is sprouted the inhibitory action that all has more than 60%.Wherein nano-emulsion 1 and nano-emulsion 4 are being used under 800 times of liquor amounts, and its effect that suppresses the yellow bacterium Microsclerotia sprouting of withering is more than 70%, and all the other reach more than 60%.
3, the mould clever nano-emulsion of evil of the present invention is to the inhibition test of verticillium dahliae wheel branch spore germination
Table 3 is disliked mould clever nano-emulsion to the impact of verticillium dahliae wheel branch spore germination
Demonstrate from table 3, compare with sterile water contrast, disliking mould clever nano-emulsion for three kinds all has good inhibitory action to the sprouting of verticillium dahliae spore.Wherein, three kinds dislike mould clever nano-emulsion original content to the inhibiting rate of verticillium dahliae spore germination all more than 94%, between 88.73-89.77%, the inhibiting rate of the nano-emulsion of 1/1000 concentration is also more than 80% to the inhibiting rate of verticillium dahliae spore germination for the nano-emulsion of 1/500 concentration.
Claims (3)
1. dislike mould clever nano-emulsion for one kind, it is characterized in that: comprise and dislike mould clever 2.0wt% ~ 9.0wt %, cyclohexanone 5.0wt% ~ 20.0wt%, hexylene glycol 3.0wt% ~ 10.0wt%, dimethicone 2.0wt% ~ 5.0wt%, polyoxyethylene nonylphenol ether phosphate 10.0wt% ~ 20.0wt%, water surplus.
2. the mould clever nano-emulsion of evil according to claim 1, it is characterized in that: the mould clever content of described evil is 5.0wt%.
3. a method for preparing claim 1 or the mould clever nano-emulsion of 2 described evils comprises the steps:
(1) the hymexazol medicine is dissolved in the solvent cyclohexanone;
(2) solution and the polyoxyethylene nonylphenol ether phosphate mix and blend of step (1) is abundant, form uniform oil phase;
(3) under stirring condition, water is joined in the oil phase of step (2), add simultaneously an amount of hexylene glycol and dimethicone, freely disperse to obtain the mould clever nano-emulsion of evil of homogeneous phase transparent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110106177 CN102217607B (en) | 2011-04-27 | 2011-04-27 | Hymexazol nanoemulsion and formulation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110106177 CN102217607B (en) | 2011-04-27 | 2011-04-27 | Hymexazol nanoemulsion and formulation method |
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| Publication Number | Publication Date |
|---|---|
| CN102217607A CN102217607A (en) | 2011-10-19 |
| CN102217607B true CN102217607B (en) | 2013-04-17 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 201110106177 Expired - Fee Related CN102217607B (en) | 2011-04-27 | 2011-04-27 | Hymexazol nanoemulsion and formulation method |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104054699B (en) * | 2014-06-26 | 2016-03-02 | 中国热带农业科学院环境与植物保护研究所 | Dislike mould Lingshui Spring agent and preparation method thereof and prevent and treat the application in banana blight with it |
| CN111149805A (en) * | 2019-12-27 | 2020-05-15 | 河北工程大学 | Pyrimethanil nanoemulsion and preparation method thereof |
| CN111357757A (en) * | 2019-12-30 | 2020-07-03 | 河北工程大学 | Acetamiprid nanoemulsion and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1253726A (en) * | 1999-11-24 | 2000-05-24 | 张天良 | Ludi and hymexazol two-component compounded fungicide |
| CN101632370A (en) * | 2009-08-17 | 2010-01-27 | 青岛星牌作物科学有限公司 | Sterilization combination for preventing and curing downy mildew of fruits and vegetables and aqueous emulsion thereof and preparation method thereof |
| CN102007912A (en) * | 2010-11-30 | 2011-04-13 | 陕西美邦农药有限公司 | Cyprodinil-containing germicide composition |
-
2011
- 2011-04-27 CN CN 201110106177 patent/CN102217607B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1253726A (en) * | 1999-11-24 | 2000-05-24 | 张天良 | Ludi and hymexazol two-component compounded fungicide |
| CN101632370A (en) * | 2009-08-17 | 2010-01-27 | 青岛星牌作物科学有限公司 | Sterilization combination for preventing and curing downy mildew of fruits and vegetables and aqueous emulsion thereof and preparation method thereof |
| CN102007912A (en) * | 2010-11-30 | 2011-04-13 | 陕西美邦农药有限公司 | Cyprodinil-containing germicide composition |
Non-Patent Citations (3)
| Title |
|---|
| 恶霉灵;晓岫;《湖南农业》;20100430;第13页 * |
| 晓岫.恶霉灵.《湖南农业》.2010,第13页. |
| 范尉尉，等.棉花根部损伤程度对感染黄萎病菌后体内抗性酶的影响.《中国农业科学》.2006,第39卷(第12期),第2483-2490页. * |
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