CN102217607A - Hymexazol nanoemulsion and formulation method - Google Patents
Hymexazol nanoemulsion and formulation method Download PDFInfo
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- CN102217607A CN102217607A CN2011101061776A CN201110106177A CN102217607A CN 102217607 A CN102217607 A CN 102217607A CN 2011101061776 A CN2011101061776 A CN 2011101061776A CN 201110106177 A CN201110106177 A CN 201110106177A CN 102217607 A CN102217607 A CN 102217607A
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- hymexazol
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Abstract
The invention provides a hymexazol nanoemulsion capable of preventing and treating cotton verticillium wilt and a formulation method of the nanoemulsion. The hymexazol nanoemulsion comprises 2.0wt%-9.0wt% of hymexazol, 5.0wt%-20.0wt% of cyclohexanone, 3.0wt%-10.0wt% of antifreeze agent, 2.0wt%-5.0wt% of defoaming agent, 10.0wt%-20.0wt% of emulsifying agent and the balance of water. As the hymexazol nanoemulsion has inhibitory action on the growth of cotton verticillium wilt bacteria hypha, the micro sclerotium germination of cotton verticillium wilt bacteria, and favorable inhibitory action on the germination of cotton verticillium wilt bacteria spore, the hymexazol nanoemulsion can well inhibit the attach probability of cotton verticillium wilt. Adn the hymexazol nanoemulsion is simple in preparation, and available for large-scale production and application, and dramatically boosts the cotton output of China.
Description
Technical field
The present invention relates to a kind of mould clever nano-emulsion of evil and compound method thereof that is used to prevent and treat cotton verticillium wilt.
Background technology
The important economic crops of cotton China, in recent years, cotton verticillium wilt increases the weight of year by year, serious contusion the cotton grower plant cotton enthusiasm.Particularly 1993,1995,1996,2002,2003,2004 etc. continuously in the great outburst of northern cotton region, wherein 1993 is especially big generation year, onset area reaches more than 50% of cultivated area, loses about 40,000 tons of gined cotton then, causes grave danger to Cotton Production.Why this disease can cause harm and be difficult to control so rampantly, and its main cause is that its pathogen can be on the crop invalid body, in addition in the medium-term and long-term survival of soil for 10 years, and the bacterium amount can accumulate throughout the year.Verticillium wilt has become the main barrier factors that influences output of cotton and quality.Verticillium wilt belongs to that soil passes, vascular bundle diseases, and cultivating disease-resistant variety is this sick main method of control, but because factors such as high anti-or resisting verticillium upland cotton antigen shortage, the breeding for disease resistance effect is unsatisfactory.
Adopt modern genetic engineering breeding method the end of the nineties in last century, list country " 863 " plan in, use pollen tube passage method, disease-resistant gene is imported cotton, breeding resistant variety, but do not have real resisting verticillium kind really up to the mark to use on producing so far.As seen, the resisting verticillium breeding process is slower, can't resolve to produce to go up the task of top priority.In the agricultural measures, though crop rotation especially rice field-upland field rotation can effectively suppress the generation of verticillium wilt, be subjected to the restriction of natural conditions or the influence of cropping system, this technology is difficult to generally be promoted.Chemical control does not also have comparatively ideal control cotton verticillium wilt efficacious agents so far aborning in the chemical control.Biological control is effective measures of preventing and treating soil-borne disease, yet the practice of control for many years shows, only screen the biocontrol microorganisms that this traditional trilogy obtains by indoor flat plate face-off screening, the living survey screening in greenhouse and field, how unstable its field efficacy is between year, greatly limited biological control and really developed into commercialization from laboratory stage.
Summary of the invention
The invention provides a kind of mould clever nano-emulsion of evil and the compound method thereof that can prevent and treat cotton verticillium wilt.
The mould clever nano-emulsion of evil of the present invention comprises and dislikes mould clever 2.0wt%～9.0wt%, cyclohexanone 5.0wt%～20.0wt%, antifreeze 3.0wt%～10.0wt%, defoamer 2.0wt%～5.0wt%, emulsifier 10.0wt%～20.0wt%, water surplus.
Wherein, preferred, the mould clever content of described evil is 5.0wt%.
Wherein, described emulsifier is a polyoxy alkene ether phosphate class.
Wherein, described emulsifier is the polyoxyethylene nonylphenol ether phosphate.
Wherein, described antifreeze is a hexylene glycol.
Wherein, described defoamer is a dimethicone.
The preparation method of the mould clever nano-emulsion of evil of the present invention comprises the steps:
(1) the hymexazol medicine is dissolved in the solvent cyclohexanone;
(2) solution and the emulsifier with step (1) stirs fully, forms uniform oil phase;
(3) under stirring condition, water is joined in the oil phase of step (2), add an amount of antifreeze and defoamer simultaneously, freely disperse to obtain the mould clever nano-emulsion of evil of homogeneous phase transparent.
Wherein, described emulsifier is a polyoxy alkene ether phosphate class.
Wherein, described emulsifier is the polyoxyethylene nonylphenol ether phosphate.
Wherein, described antifreeze is a hexylene glycol.
Wherein, described defoamer is a dimethicone.
Beneficial effect of the present invention is as follows:
Because the mould clever nano-emulsion of evil of the present invention has inhibitory action to the verticillium dahliae mycelial growth; Sprouting has inhibitory action to verticillium dahliae germ nuclear; Sprouting to the verticillium dahliae spore has good inhibition effect, therefore the mould clever nano-emulsion of evil of the present invention can suppress the incidence probability of cotton verticillium wilt well, and the mould clever nano-emulsion preparation of evil of the present invention is simple, can large batch of production and application, significantly improve the output of Cotton in China.
Embodiment
The compound method of the mould clever nano-emulsion of evil of the present invention is as follows:
Embodiment 1
The hymexazol medicine 0.2g of 95wt% is dissolved in the 0.4mL cyclohexanone solvent, and fully dissolving adds emulsifier polyoxyethylene nonylphenol ether phosphoesterase 30 .4mL; Be stirred into the transparent body, add antifreeze hexylene glycol 0.2mL and defoamer dimethicone 0.1mL afterwards, add running water 2.5mL simultaneously, stir into the transparent body, be and dislike mould clever nano-emulsion 1, this dislikes consisting of of mould clever nano-emulsion 1: dislike mould clever 5.0wt%, cyclohexanone 10.5wt%, antifreeze 5.0wt%, defoamer 2.6wt%, emulsifier 10.5wt%, water surplus.
Embodiment two:
0.2g is dissolved in the 0.6mL cyclohexanone solvent with 95wt% hymexazol medicine, and fully dissolving adds emulsifier polyoxyethylene nonylphenol ether phosphoesterase 30 .4mL; Be stirred into the transparent body, add antifreeze hexylene glycol 0.2mL and defoamer dimethicone 0.1mL afterwards, add running water 2.3mL simultaneously, stir into the transparent body, be and dislike mould clever nano-emulsion 2, this dislikes consisting of of mould clever nano-emulsion 2: getting above-mentioned component density is 1, dislikes mould clever 5.0wt%, cyclohexanone 15.8wt%, antifreeze 5.0wt%, defoamer 2.6wt%, emulsifier 10.5wt%, water surplus.
Embodiment three
The hymexazol medicine 0.2g of 95wt% is dissolved in the 0.5mL cyclohexanone solvent, and fully dissolving adds emulsifier polyoxyethylene nonylphenol ether phosphate 1.5mL; Be stirred into the transparent body, add antifreeze hexylene glycol 0.6mL and defoamer dimethicone 0.3mL afterwards, add running water 6.4mL simultaneously, stir into the transparent body, be and dislike mould clever nano-emulsion 3, this dislikes consisting of of mould clever nano-emulsion 3: getting above-mentioned component density is 1, dislikes mould clever 2.0wt%, cyclohexanone 5.3wt%, antifreeze 6.3wt%, defoamer 3.2wt%, emulsifier 15.8wt%, water surplus.
Embodiment four
The hymexazol medicine 0.2g of 95wt% is dissolved in the 0.4mL cyclohexanone solvent, and fully dissolving adds emulsifier polyoxyethylene nonylphenol ether phosphoesterase 30 .4mL; Be stirred into the transparent body, add antifreeze hexylene glycol 0.2mL and defoamer dimethicone 0.1mL afterwards, add running water 0.8mL simultaneously, stir into the transparent body, be and dislike mould clever nano-emulsion 4, this dislikes consisting of of mould clever nano-emulsion 4: getting above-mentioned component density is 1, dislikes mould clever 9.0wt%, cyclohexanone 19.0wt%, antifreeze 9.5wt%, defoamer 4.8wt%, emulsifier 19.0wt%, water surplus.
Of the present invention above-mentioned three kinds dislike mould clever nano-emulsions 1,3,4 (, only selecting nano-emulsion 1 to be experimental subjects) because it is identical to dislike the content of mould spirit in the nano-emulsion 1 and 2 to cotton verticillium wilt to prevent and treat experimental data as follows:
1, the mould clever nano-emulsion of evil of the present invention is to the inhibition test of verticillium dahliae mycelial growth.
Table 1 is disliked the inhibition effect of mould clever nano-emulsion to the verticillium dahliae mycelial growth
From table 1, demonstrate, contrast, dislike mould clever nano-emulsion various dose the verticillium dahliae mycelial growth is all had inhibitory action more than 60% for above-mentioned three kinds with clear water (sterile water).Wherein nano-emulsion 1 is being used under 5 kinds of various dose, and it suppresses verticillium wilt pathogen mycelial growth effect all more than 65%, and nano-emulsion 4 suppresses effect when using five kinds of dosage suitable with nano-emulsion 1.
2, the mould clever nano-emulsion of evil of the present invention is to the inhibition test of verticillium dahliae germ nuclear sprouting
The influence that table 2 nano-emulsion is sprouted verticillium dahliae germ nuclear
From table 2, demonstrate, compare, dislike mould clever nano-emulsion various dose for three kinds verticillium dahliae germ nuclear is sprouted the inhibitory action that all has more than 60% with the sterile water contrast.Wherein nano-emulsion 1 and nano-emulsion 4 are being used under 800 times of liquor amounts, and its effect that suppresses the yellow bacterium germ nuclear sprouting of withering is more than 70%, and all the other reach more than 60%.
3, the mould clever nano-emulsion of evil of the present invention is to the inhibition test of verticillium dahliae wheel branch spore germination
Table 3 is disliked the influence of mould clever nano-emulsion to the spore germination of verticillium dahliae wheel branch
Demonstrate from table 3, compare with sterile water contrast, disliking mould clever nano-emulsion for three kinds all has good inhibition effect to the sprouting of verticillium dahliae spore.Wherein, three kinds dislike mould clever nano-emulsion original content to the inhibiting rate of verticillium dahliae spore germination all more than 94%, between 88.73-89.77%, the inhibiting rate of the nano-emulsion of 1/1000 concentration is also more than 80% to the inhibiting rate of verticillium dahliae spore germination for the nano-emulsion of 1/500 concentration.
Claims (10)
1. dislike mould clever nano-emulsion for one kind, it is characterized in that: comprise and dislike mould clever 2.0wt%～9.0wt%, cyclohexanone 5.0wt%～20.0wt%, antifreeze 3.0wt%～10.0wt%, defoamer 2.0wt%～5.0wt%, emulsifier 10.0wt%～20.0wt%, water surplus.
2. the mould clever nano-emulsion of evil according to claim 1 is characterized in that: the mould clever content of described evil is 5.0wt%.
3. the mould clever nano-emulsion of evil according to claim 1 and 2 is characterized in that: described emulsifier is a polyoxy alkene ether phosphate class.
4. the mould clever nano-emulsion of evil according to claim 3 is characterized in that: described emulsifier is the polyoxyethylene nonylphenol ether phosphate.
5. the mould clever nano-emulsion of evil according to claim 1 and 2 is characterized in that: described antifreeze is a hexylene glycol.
6. the mould clever nano-emulsion of evil according to claim 1 and 2 is characterized in that: described defoamer is a dimethicone.
7. a preparation method who dislikes mould clever nano-emulsion comprises the steps:
(1) the hymexazol medicine is dissolved in the solvent cyclohexanone;
(2) solution and the emulsifier with step (1) stirs fully, forms uniform oil phase;
(3) under stirring condition, water is joined in the oil phase of step (2), add an amount of antifreeze and defoamer simultaneously, freely disperse to obtain the mould clever nano-emulsion of evil of homogeneous phase transparent.
8. the preparation method of the mould clever nano-emulsion of evil according to claim 7 is characterized in that: described emulsifier is a polyoxy alkene ether phosphate class.
9. the preparation method of the mould clever nano-emulsion of evil according to claim 8 is characterized in that: described emulsifier is the polyoxyethylene nonylphenol ether phosphate.
10. the preparation method of the mould clever nano-emulsion of evil according to claim 7 is characterized in that: described antifreeze is a hexylene glycol, and described defoamer is a dimethicone.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110106177 CN102217607B (en) | 2011-04-27 | 2011-04-27 | Hymexazol nanoemulsion and formulation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110106177 CN102217607B (en) | 2011-04-27 | 2011-04-27 | Hymexazol nanoemulsion and formulation method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102217607A true CN102217607A (en) | 2011-10-19 |
| CN102217607B CN102217607B (en) | 2013-04-17 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110106177 Expired - Fee Related CN102217607B (en) | 2011-04-27 | 2011-04-27 | Hymexazol nanoemulsion and formulation method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104054699A (en) * | 2014-06-26 | 2014-09-24 | 中国热带农业科学院环境与植物保护研究所 | Hymexazol aqueous solution and preparation method and application thereof to control of banana wilt |
| CN111149805A (en) * | 2019-12-27 | 2020-05-15 | 河北工程大学 | Pyrimethanil nanoemulsion and preparation method thereof |
| CN111357757A (en) * | 2019-12-30 | 2020-07-03 | 河北工程大学 | Acetamiprid nanoemulsion and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1253726A (en) * | 1999-11-24 | 2000-05-24 | 张天良 | Ludi and hymexazol two-component compounded fungicide |
| CN101632370A (en) * | 2009-08-17 | 2010-01-27 | 青岛星牌作物科学有限公司 | Sterilization combination for preventing and curing downy mildew of fruits and vegetables and aqueous emulsion thereof and preparation method thereof |
| CN102007912A (en) * | 2010-11-30 | 2011-04-13 | 陕西美邦农药有限公司 | Cyprodinil-containing germicide composition |
-
2011
- 2011-04-27 CN CN 201110106177 patent/CN102217607B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1253726A (en) * | 1999-11-24 | 2000-05-24 | 张天良 | Ludi and hymexazol two-component compounded fungicide |
| CN101632370A (en) * | 2009-08-17 | 2010-01-27 | 青岛星牌作物科学有限公司 | Sterilization combination for preventing and curing downy mildew of fruits and vegetables and aqueous emulsion thereof and preparation method thereof |
| CN102007912A (en) * | 2010-11-30 | 2011-04-13 | 陕西美邦农药有限公司 | Cyprodinil-containing germicide composition |
Non-Patent Citations (2)
| Title |
|---|
| 晓岫: "恶霉灵", 《湖南农业》 * |
| 范尉尉，等: "棉花根部损伤程度对感染黄萎病菌后体内抗性酶的影响", 《中国农业科学》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104054699A (en) * | 2014-06-26 | 2014-09-24 | 中国热带农业科学院环境与植物保护研究所 | Hymexazol aqueous solution and preparation method and application thereof to control of banana wilt |
| CN104054699B (en) * | 2014-06-26 | 2016-03-02 | 中国热带农业科学院环境与植物保护研究所 | Dislike mould Lingshui Spring agent and preparation method thereof and prevent and treat the application in banana blight with it |
| CN111149805A (en) * | 2019-12-27 | 2020-05-15 | 河北工程大学 | Pyrimethanil nanoemulsion and preparation method thereof |
| CN111357757A (en) * | 2019-12-30 | 2020-07-03 | 河北工程大学 | Acetamiprid nanoemulsion and preparation method thereof |
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| Publication number | Publication date |
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| CN102217607B (en) | 2013-04-17 |
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