CN102204475A - Lyophyllum connatum(schum.txFr.)Sing js39 strain and use thereof - Google Patents

Lyophyllum connatum(schum.txFr.)Sing js39 strain and use thereof Download PDF

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CN102204475A
CN102204475A CN2011100758487A CN201110075848A CN102204475A CN 102204475 A CN102204475 A CN 102204475A CN 2011100758487 A CN2011100758487 A CN 2011100758487A CN 201110075848 A CN201110075848 A CN 201110075848A CN 102204475 A CN102204475 A CN 102204475A
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white mushroom
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lyophyllum
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CN102204475B (en
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卢凤忠
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Abstract

The invention relates to a Lyophyllum connatum(schum.txFr.)Sing js39 strain and use thereof. The collection number of the Lyophyllum connatum(schum.txFr.)Sing js39 strain is CCTCCNo.M2011018. The Lyophyllum connatum(schum.txFr.)Sing can be cultivated in fermented material on ground or in bags, the finished Lyophyllum connatum(schum.txFr.)Sing has high nutritional value, and according to tests, the Lyophyllum connatum(schum.txFr.)Sing contains various nutrients including 18 amino acids, calcium, iron, zinc, phosphorus, fat, proteins, polysaccharides and selenium. The ground Lyophyllum connatum(schum.txFr.)Sing cultivation method has the advantages of high breathability and high fruiting speed. At 20 to 25 DEG C, the Lyophyllum connatum(schum.txFr.)Sing can fruit within 20 to 25 days normally. Packaging bags and labor are saved, and land resources are fully utilized. The feed amount, yield, production value and pure profit are three times more than those achieved by the conventional method. The feed amount, yield, production value and pure profit of the bag Lyophyllum connatum(schum.txFr.)Sing cultivation method are 7 times more than those achieved by the conventional method. In the method, crop straw such as rice straw, corncobs, broomcorn straw, wheat straw, cotton straw, sunflower straw, cottonseed hull and the like can be utilized.

Description

A kind of symphysis is from pleat umbrella circle white mushroom js39 bacterial classification and application thereof
Technical field
The present invention relates to the white mushroom bacterial classification of an Artenkreis, belong to technical field of edible fungi production.
The alleged symphysis of the present invention is from the white mushroom js39 of pleat umbrella circle called after: symphysis from the white mushroom js39 of pleat umbrella circle ( Lyophyllum connatum(schum.tx Fr.) Sing), on January 12nd, 2011 in China's typical culture collection center preservation, be numbered CCTCC NO:M 2011018.
Background technology
Enclosing white mushroom is the rare mountain delicacy on mountain range, Changbai Mountain, is grown in the thick grass of evergreen grass, forms oval-shaped fairy ring, little 100-200cm, and big 1000-2000cm encloses white mushroom and just is grown in the fairy ring, so claim circle white mushroom.
Generally acknowledge the method for the white mushroom of cultivation circle at present, cook major ingredient with corncob exactly, add certain auxiliary material, with the bacterium bag of 17 * 33cm, sterilizing kitchen sterilization in the pack is more than the sterilization 12h, gone out behind the bacterium with connecing the sterilization of bacterium case, connecing bacterium, covered with the bacterium bag about 40 days, done furrow earlier, the wide 100cm of furrow, dark 15cm, length is not limit, the band bag upright is gone into furrow, stay the space of 10cm to tamp with soil between bag and the bag, unnecessary plastic sack is cut off, fruiting waters.Every 5000kg material need be adorned surplus the bacterium bag 14000 of 17 * 33cm, and sterilization needs standard coal cinder 800kg.Every mu of shared raw material 3500kg in ground, 10000 bags in bacterium bag goes out dried mushroom 250kg.The method operation is too loaded down with trivial details, and recruitment is too many, waste energy, and the waste soil, the cost height, output is low.
Summary of the invention
In order to solve the problem of above-mentioned existence, the invention provides a kind of symphysis from white mushroom bacterial classification of pleat umbrella circle and application thereof.This bacterial classification is planted or the cultivation of bag cultivating method with adopting, and operation is simple, a bacterium is fast, output is high.
The objective of the invention is to be achieved through the following technical solutions: symphysis is characterized in that from the white mushroom js39 of pleat umbrella circle bacterial classification: a kind of symphysis from the white mushroom js39 of pleat umbrella circle ( Lyophyllum connatum(schum.tx Fr.) Sing), in China's typical culture collection center preservation, be numbered CCTCC NO:M 2011018.
A kind of method of utilizing symphysis from the white mushroom of pleat umbrella circle white mushroom js39 cultivating bacterial spawn circle, its step is as follows:
(1) batching of selecting materials: select straw, corncob, maize straw, sorghum rod, straw, the cotton bar, the sunflower bar, in cotton seed hull or the wood chip one or more mix as major ingredient, with hay cutter major ingredient is cut up with a hay cutter a cun section, add major ingredient and auxiliary material according to following weight percentage, major ingredient 55 ~ 78%, soyabean cake 1 ~ 10%, corn flour 1 ~ 10%, wheat bran 2 ~ 18%, gypsum 0.5 ~ 3%, brown sugar 0.5 ~ 3%, lime 0.5 ~ 3%, potassium dihydrogen phosphate 0.1 ~ 0.3%, magnesium sulfate 0.1 ~ 0.3% adds the water of compound weight 125 ~ 180% again after mixing, compound is drenched, mix thoroughly, as windrow;
(2) build the heap fermentation: build up high 150cm mixing uniform windrow, wide 300cm, the long trapezoidal heap of not limitting, waddy with diameter 6-8cm is beaten air-vent on stockpile, air-vent is isosceles triangle, and pitch-row is 50cm, covers with straw screen or mat above, when treating that windrow is warmed up to 60-70 ℃, keep 24h, about up and down casting, and beat air-vent again, again through 70-80h fermentation, make pH value and be 6.5 ~ 8 fermentation material;
(3) stone sowing: the fermentation material is gone into bedding cultivation, the wide 100cm of furrow, the dark 10cm of furrow, length is not limit, shop ground floor fermentation material, thick 15cm, on material was being fermented in the capable sowing of the white mushroom js39 of pleat umbrella circle bacterial classification finished product word, seed spacing was 10cm with symphysis, spread second layer fermentation material more thereon, thick 20cm is turtleback shape above, broadcasts second layer kind, with symphysis from the capable sowing of the white mushroom js39 of pleat umbrella circle bacterial classification finished product word on fermentation material, spacing is at 10cm, spreads one deck fermentation material on the bacterial classification of upper strata, more in the above behind the earthing 1.5-2cm, unregistered land film with punching covers, real with soil pressure all around, issue bacterium 20-25 ℃ temperature, got final product fruiting in 20-25 days;
(4) management of producing mushroom: temperature is controlled at 20-28 ℃ behind the fruiting, and humidity is controlled at 85-90%.
A kind of method of utilizing symphysis from the white mushroom of pleat umbrella circle white mushroom js39 cultivating bacterial spawn circle, its step is as follows:
(1) batching of selecting materials: select straw, corncob, maize straw, sorghum rod, straw, the cotton bar, the sunflower bar, in cotton seed hull or the wood chip one or more mix as major ingredient, with hay cutter major ingredient is cut up with a hay cutter a cun section, add major ingredient and auxiliary material according to following weight percentage, major ingredient 55 ~ 78%, soyabean cake 1 ~ 10%, corn flour 1 ~ 10%, wheat bran 2 ~ 18%, gypsum 0.5 ~ 3%, brown sugar 0.5 ~ 3%, lime 0.5 ~ 3%, potassium dihydrogen phosphate 0.1 ~ 0.3%, magnesium sulfate 0.1 ~ 0.3% adds the water of compound weight 125 ~ 180% again after mixing, compound is drenched, mix thoroughly, as windrow;
(2) build the heap fermentation: build up high 150cm mixing uniform windrow, wide 300cm, the long trapezoidal heap of not limitting, waddy with diameter 6-8cm is beaten air-vent on stockpile, air-vent is isosceles triangle, and pitch-row is 50cm, covers with straw screen or mat above, when treating that windrow is warmed up to 60-70 ℃, keep 24h, about up and down casting, and beat air-vent again, again through 70-80h fermentation, make pH value and be 6.5 ~ 8 fermentation material;
(3) pack sowing: pack into the plastics bacterium bag of 25 * 52cm of the fermentation material that above-mentioned steps is made, when adorning half bag, add one deck symphysis from pleat umbrella circle white mushroom js39 bacterial classification and compacting, add one deck symphysis again from pleat umbrella circle white mushroom js39 bacterial classification and compacting after will putting in order packed expiring, one side pocket mouth of plastics bacterium bag is put with lasso, sack turns in the lasso outside, with the paper lid in the above, and sack tightened, again plastics bacterium bag is turned around and add one deck symphysis again from pleat umbrella circle white mushroom js39 bacterial classification and compacting, the opposite side sack of plastics bacterium bag is put with lasso, sack turns in the lasso outside, covers in the above with paper, and sack is tightened, temperature at 20-25 ℃ of degree issues bacterium, gets final product fruiting in 20-25 days;
(4) management of producing mushroom: temperature is controlled at 20-28 ℃ behind the fruiting, and humidity is controlled at 85-90%.
Beneficial effect of the present invention: symphysis provided by the invention from the white mushroom js39 of pleat umbrella circle ( Lyophyllum connatum(schum.tx Fr.) Sing) bacterial classification can be cultivated the white mushroom of circle by the mode that ground plants or bag is planted, and encloses white mushroom finished product and is of high nutritive value, and contains 18 seed amino acids after testing, calcium, iron, zinc, phosphorus, fat, protein, polysaccharide, the selenium multiple nutritional components, wherein polyoses content reaches 3.05g/100g.Plant the white mushroom method of circle provided by the inventionly, have good permeability, send out the fast advantage of bacterium, under the temperature of 20-25 ℃ of degree, normally got final product fruiting in 20-25 days.Every mu of ground useful raw materials 10836kg goes out dried mushroom 800kg.Economize pack, connect the sterilization of bacterium case, connect bacterium, artificial, standard coal cinder, made full use of land resources.Inventory, output, the output value, net profit also are more than three times that generally acknowledge simultaneously.Bag provided by the invention is planted the white mushroom method of circle, and every mu of ground 26350kg that can feed intake can adorn 15500 in the bacterium bag of 25 * 52cm.Every mu of ground packs artificial 2/3 than the province that generally acknowledges, economize bacterium bag, dress sterilizing kitchen, bacterium bag and play sterilizing kitchen, bacterium bag bacterial classification case to connect bacterium etc. artificial, economizes coal 4150kg.Be both that 0.07 hectare feeds intake more, output, the output value, net profit also is more than 7 times that generally acknowledge.In addition the inventive method stalk straw, corncob, maize straw, sorghum rod, straw, cotton bar, sunflower bar, cotton seed hull geometric ratio light that can utilize all crops with corncob decide base many again a lot of raw materials.The stalk of crops has every year, is a recycling economy.
Embodiment
Symphysis of the present invention from the white mushroom js39 of pleat umbrella circle ( Lyophyllum connatum(schum.tx Fr.) Sing) be the white mushroom of circle of gathering size in the open air, through cultivation, separate the symphysis that obtains high yield, anti-assorted bacterium, delicious flavour from the white mushroom js39 of pleat umbrella circle ( Lyophyllum connatum(schum.tx Fr.) Sing) bacterial classification.
It is as follows from the method for the white mushroom js39 of pleat umbrella circle to separate symphysis from the white mushroom of wild circle: the white mushroom of wild circle is placed on the mother culture media, and the batching of mother culture media is as follows: potato 200g, white sugar 20g, agar 20g, magnesium sulfate 1g, potassium dihydrogen phosphate 2g, peptone 2g, yeast extract 2g, water 1000mL.With mother culture media cooked back packing 20 * 200mm test tube of preparing burden, behind autoclaving, in sterile board, connect bacterium, 20-25 ℃ cultivate down can grow in 15 days symphysis from the white mushroom js39 of pleat umbrella circle ( Lyophyllum connatum(schum.tx Fr.) Sing) bacterial classification.
The symphysis that to cultivate out by the above-mentioned steps mother culture media from the white mushroom js39 of pleat umbrella circle ( Lyophyllum connatum(schum.tx Fr.) Sing) bacterial classification is placed on the pedigree seed culture medium, continue to cultivate, the pedigree seed culture medium batching is as follows: corncob 75g, corn flour 4g, soyabean cake 4g, wheat bran 13g, brown sugar 1g, gypsum 1g, lime 1.5g, magnesium sulfate 0.2g, potassium dihydrogen phosphate 0.3g, water 130g.Mix the bacterium bag of packing 17 * 28cm behind the honest material, in the normal-pressure sterilization kitchen range, in sterile board, connect bacterium behind the sterilization 12h, cultivate down at 20-25 ℃ and can cultivate symphysis from the white mushroom js39 of pleat umbrella circle bacterial initial species in 25 days.
The symphysis that to be cultivated out by the above-mentioned steps mother culture media is from the white mushroom js39 of pleat umbrella circle (Lyophyllum connatum(schum.tx Fr.) Sing) bacterial initial species is placed on and produces on kind of the medium, continue to cultivate, produce kind of medium and prepare burden as follows: corncob 75g, corn flour 4g, soyabean cake 4g, wheat bran 13g, brown sugar 1g, gypsum 1g, lime 1.5g, magnesium sulfate 0.2g, potassium dihydrogen phosphate 0.3g, water 130g.In sterile board, connect bacterium, cultivate down at 20-25 ℃ and can cultivate symphysis from the white mushroom js39 of pleat umbrella circle bacterial classification production kind in 25 days.
Embodiment 1:
Describe in detail below utilize symphysis from the white mushroom js39 of pleat umbrella circle ( Lyophyllum connatum(schum.tx Fr.) Sing) bacterial classification is planted the method for the white mushroom of method cultivation circle with adopting, and its step is as follows:
(1) batching of selecting materials: select straw, corncob, maize straw, sorghum rod, straw, the cotton bar, the sunflower bar, in cotton seed hull or the wood chip one or more mix as major ingredient, note to select no industrial pollution, pesticide pollution, the stalk that fresh nothing is gone mouldy is cooked major ingredient, with hay cutter major ingredient is cut up with a hay cutter a cun section, add major ingredient and auxiliary material according to following weight, major ingredient 58kg, soyabean cake 10kg, corn flour 8kg, wheat bran 18kg, gypsum 2.5kg, brown sugar 2kg, lime 1kg, potassium dihydrogen phosphate 0.3kg, magnesium sulfate 0.2kg, add the water of 150kg after major ingredient and auxiliary material mixed again, compound is drenched, mix thoroughly, as windrow;
(2) build the heap fermentation: build up high 150cm mixing uniform windrow, wide 300cm, the long trapezoidal heap of not limitting, waddy with diameter 6-8cm is beaten air-vent on stockpile, air-vent is isosceles triangle, and pitch-row is 50cm, cover with straw screen or mat above, about 3-4 days, when treating that windrow is warmed up to 60-70 ℃, keep 24h, about up and down casting, and beat air-vent again, through the 72h fermentation, at this moment composts or fertilisers of cultivating is bronzing, and overflows with wine flavour again, through measuring pH value is 7, illustrates that the fermentation material prepares;
(3) stone sowing: the fermentation material is gone into bedding cultivation, the wide 100cm of furrow, the dark 10cm of furrow, length is not limit, and stays 30cm operation road; The area of the antecedent soil moisture water at the bottom of one deck of can splashing, every square meter can spread the 0.1kg lime, is used to prevent and treat soil insect.Shop ground floor fermentation material, thick 15cm, with symphysis from the capable sowing of the white mushroom js39 of pleat umbrella circle bacterial classification production kind finished product word on fermentation material, seed spacing is 10cm, spread second layer fermentation material more thereon, thick 20cm, be turtleback shape above, broadcast second layer kind, on material was being fermented in the capable sowing of the white mushroom js39 of pleat umbrella circle bacterial classification production kind finished product word, spacing was at 10cm with symphysis, spread skim fermentation material above, is principle with bacterial classification like revealing like not revealing, and covers following moistening soil (hold tightly with hand, landing scatters the is standard) 1.5-2cm of face of land 10cm more in the above.Spray the chrysanthemum lipid insecticide of 1500 times of high-efficiency low-toxicities, the unregistered land film of upper cover punching gets final product with soil pressure is real all around.When in the open country or the woods, planting, the straw sunshade of lid layer 3-5cm more above.Temperature at 20-25 ℃ issues bacterium, normally gets final product fruiting in 20-25 days;
(4) management of producing mushroom: as can be directly in brooder or cold canopy with woven straw control temperature about 20-28 ℃, illumination is controlled at 3 fens positive 7 seconds for well, humidity at 85-90% for well.All right as in the woods, utilizing natural lighting, as on land, can put up sunshade with the sunshade net in the above, illumination was standard with 3 fens positive 7 seconds in a word, relative air humidity is standard with 85-90%, ventilate with refrigerant not gloomy be standard, normally can gather 5 batches.
Adopt the present invention ground to plant the white mushroom of method cultivation circle, total every square meter 1.25kg of sowing quantity, the every square meter 21kg that feeds intake, 666 ㎡ (mu area)-150 ㎡ (operation road area)=516 ㎡ (every mu usable area).Every mu of real feeding intake in ground: 516 ㎡ * 21kg=10836kg go out dried mushroom 800kg.
Embodiment 2:
Describe in detail below utilize symphysis from the white mushroom js39 of pleat umbrella circle ( Lyophyllum connatum(schum.tx Fr.) Sing) bacterial classification adopts the method for the white mushroom of bag cultivation method cultivation circle, and its step is as follows:
(1) batching of selecting materials: select straw, corncob, maize straw, sorghum rod, straw, the cotton bar, the sunflower bar, in cotton seed hull or the wood chip one or more mix as major ingredient, note to select no industrial pollution, pesticide pollution, the stalk that fresh nothing is gone mouldy is cooked major ingredient, with hay cutter major ingredient is cut up with a hay cutter a cun section, add major ingredient and auxiliary material according to following weight, major ingredient 78kg, soyabean cake 2kg, corn flour 5kg, wheat bran 10kg, gypsum 0.8kg, brown sugar 1.8kg, lime 2kg, potassium dihydrogen phosphate 0.3kg, magnesium sulfate 0.1kg, the water that adds compound weight 140kg after mixing again is drenched with compound, mix thoroughly, as windrow;
(2) build the heap fermentation: build up high 150cm mixing uniform windrow, wide 300cm, the long trapezoidal heap of not limitting, waddy with diameter 6-8cm is beaten air-vent on stockpile, air-vent is isosceles triangle, and pitch-row is 50cm, cover with straw screen or mat above, about 3-4 days, when treating that windrow is warmed up to 60-70 ℃, keep 24h, about up and down casting, and beat air-vent again, through the 72h fermentation, at this moment composts or fertilisers of cultivating is bronzing, and overflows with wine flavour again, through measuring pH value is 8, illustrates that the fermentation material prepares;
(3) pack sowing: pack into the plastics bacterium bag of 25 * 52cm of the fermentation material that above-mentioned steps is made, when adorning half bag, add one deck symphysis from pleat umbrella circle white mushroom js39 bacterial classification and compacting, add one deck symphysis again from the white mushroom js39 of pleat umbrella circle bacterial classification production kind and compacting after will putting in order packed expiring, one side pocket mouth of plastics bacterium bag is put with lasso, sack turns in the lasso outside, with the paper lid in the above, and sack is tightened with leather sheath, again plastics bacterium bag is turned around and add one deck symphysis again from the white mushroom js39 of pleat umbrella circle bacterial classification production kind and compacting, the opposite side sack of plastics bacterium bag is put with lasso, and sack turns in the lasso outside, with the paper lid in the above, and sack is tightened with leather sheath, three layers of kind of two layered materials, the complete open bacterium that connects is placed on culturing room and cultivates after installing.Under the temperature of 20-25 ℃ of degree, cultivate, normally got final product fruiting in 20-25 days.Temperature at 20-25 ℃ of degree issues bacterium, gets final product fruiting in 20-25 days;
(4) management of producing mushroom: can become 7-8 high bacterium buttress to the bacterium bag sign indicating number of sending out bacterium good, stay the passageway of 70cm between bacterium buttress and the bacterium buttress, be used to adopt mushroom and the usefulness that keeps the skin wet.Illumination was standard with 3 fens positive 7 seconds, and temperature is controlled at 20-28 ℃, and relative air humidity is standard with 85-90%, ventilate with refrigerant not gloomy be standard, normally can gather 5 batches;
Adopt the white mushroom of bag cultivation method of the present invention cultivation circle, the every bag of 1.7kg that can feed, every 5000kg material can adorn 3000 every bag in the bacterium bag 1.7kg that can feed, and dress up the bacterium bag of long 35cm * diameter 16cm.Every mu of ground can be piled up 15500 bags, every mu of ground 15500 bags * 1.7kg=26350kg that can feed intake.Go out dried mushroom 1800kg.

Claims (3)

1. a symphysis is characterized in that from the white mushroom js39 of pleat umbrella circle bacterial classification: symphysis from the white mushroom js39 of pleat umbrella circle ( Lyophyllum connatum(schum.tx Fr.) Sing), in China's typical culture collection center preservation, be numbered CCTCC NO:M 2011018.
2. method of utilizing the described symphysis of claim 1 from the white mushroom of pleat umbrella circle white mushroom js39 cultivating bacterial spawn circle, its step is as follows:
(1) batching of selecting materials: select straw, corncob, maize straw, sorghum rod, straw, the cotton bar, the sunflower bar, in cotton seed hull or the wood chip one or more mix as major ingredient, with hay cutter major ingredient is cut up with a hay cutter a cun section, add major ingredient and auxiliary material according to following weight percentage, major ingredient 55 ~ 78%, soyabean cake 1 ~ 10%, corn flour 1 ~ 10%, wheat bran 2 ~ 18%, gypsum 0.5 ~ 3%, brown sugar 0.5 ~ 3%, lime 0.5 ~ 3%, potassium dihydrogen phosphate 0.1 ~ 0.3%, magnesium sulfate 0.1 ~ 0.3% adds the water of compound weight 125 ~ 180% again after mixing, compound is drenched, mix thoroughly, as windrow;
(2) build the heap fermentation: build up high 150cm mixing uniform windrow, wide 300cm, the long trapezoidal heap of not limitting, waddy with diameter 6-8cm is beaten air-vent on stockpile, air-vent is isosceles triangle, and pitch-row is 50cm, covers with straw screen or mat above, when treating that windrow is warmed up to 60-70 ℃, keep 24h, about up and down casting, and beat air-vent again, again through 70-80h fermentation, make pH value and be 6.5 ~ 8 fermentation material;
(3) stone sowing: the fermentation material is gone into bedding cultivation, the wide 100cm of furrow, the dark 10cm of furrow, length is not limit, shop ground floor fermentation material, thick 15cm, on material was being fermented in the capable sowing of the white mushroom js39 of pleat umbrella circle bacterial classification finished product word, seed spacing was 10cm with symphysis, spread second layer fermentation material more thereon, thick 20cm is turtleback shape above, broadcasts second layer kind, with symphysis from the capable sowing of the white mushroom js39 of pleat umbrella circle bacterial classification finished product word on fermentation material, spacing is at 10cm, spreads one deck fermentation material on the bacterial classification of upper strata, more in the above behind the earthing 1.5-2cm, unregistered land film with punching covers, real with soil pressure all around, issue bacterium 20-25 ℃ temperature, got final product fruiting in 20-25 days;
(4) management of producing mushroom: temperature is controlled at 20-28 ℃ behind the fruiting, and humidity is controlled at 85-90%.
3. method of utilizing the described symphysis of claim 1 from the white mushroom of pleat umbrella circle white mushroom js39 cultivating bacterial spawn circle, its step is as follows:
(1) batching of selecting materials: select straw, corncob, maize straw, sorghum rod, straw, the cotton bar, the sunflower bar, in cotton seed hull or the wood chip one or more mix as major ingredient, with hay cutter major ingredient is cut up with a hay cutter a cun section, add major ingredient and auxiliary material according to following weight percentage, major ingredient 55 ~ 78%, soyabean cake 1 ~ 10%, corn flour 1 ~ 10%, wheat bran 2 ~ 18%, gypsum 0.5 ~ 3%, brown sugar 0.5 ~ 3%, lime 0.5 ~ 3%, potassium dihydrogen phosphate 0.1 ~ 0.3%, magnesium sulfate 0.1 ~ 0.3% adds the water of compound weight 125 ~ 180% again after mixing, compound is drenched, mix thoroughly, as windrow;
(2) build the heap fermentation: build up high 150cm mixing uniform windrow, wide 300cm, the long trapezoidal heap of not limitting, waddy with diameter 6-8cm is beaten air-vent on stockpile, air-vent is isosceles triangle, and pitch-row is 50cm, covers with straw screen or mat above, when treating that windrow is warmed up to 60-70 ℃, keep 24h, about up and down casting, and beat air-vent again, again through 70-80h fermentation, make pH value and be 6.5 ~ 8 fermentation material;
(3) pack sowing: pack into the plastics bacterium bag of 25 * 52cm of the fermentation material that above-mentioned steps is made, when adorning half bag, add one deck symphysis from pleat umbrella circle white mushroom js39 bacterial classification and compacting, add one deck symphysis again from pleat umbrella circle white mushroom js39 bacterial classification and compacting after will putting in order packed expiring, one side pocket mouth of plastics bacterium bag is put with lasso, sack turns in the lasso outside, with the paper lid in the above, and sack tightened, again plastics bacterium bag is turned around and add one deck symphysis again from pleat umbrella circle white mushroom js39 bacterial classification and compacting, the opposite side sack of plastics bacterium bag is put with lasso, sack turns in the lasso outside, covers in the above with paper, and sack is tightened, temperature at 20-25 ℃ of degree issues bacterium, gets final product fruiting in 20-25 days;
(4) management of producing mushroom: temperature is controlled at 20-28 ℃ behind the fruiting, and humidity is controlled at 85-90%.
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CN103210849A (en) * 2013-03-04 2013-07-24 上海丰科生物科技股份有限公司 Lyophyllum decastes new bacterial strain
CN103210849B (en) * 2013-03-04 2014-07-09 上海丰科生物科技股份有限公司 Lyophyllum decastes new bacterial strain
CN103340156A (en) * 2013-03-25 2013-10-09 上海丰科生物科技股份有限公司 Novel strain of lyophyllum decastes
CN103483063A (en) * 2013-09-06 2014-01-01 攀枝花市农林科学研究院 Culture medium using waste walnut twigs as matrix as well as preparation method and application thereof
CN106034739A (en) * 2016-06-02 2016-10-26 蚌埠市蚌山区海上明珠家庭农场 Imitating-wild culturing method of agaricus bisporus
CN111264303A (en) * 2020-04-09 2020-06-12 中华全国供销合作总社昆明食用菌研究所 Lyophyllum fumosoroseum cultivar and preparation method thereof

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