CN102191288B - 赖氨酸的反式发酵制备 - Google Patents
赖氨酸的反式发酵制备 Download PDFInfo
- Publication number
- CN102191288B CN102191288B CN2011100656943A CN201110065694A CN102191288B CN 102191288 B CN102191288 B CN 102191288B CN 2011100656943 A CN2011100656943 A CN 2011100656943A CN 201110065694 A CN201110065694 A CN 201110065694A CN 102191288 B CN102191288 B CN 102191288B
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- CN
- China
- Prior art keywords
- pyridine nucleotide
- nucleotide transhydrogenase
- fermentation process
- bacterium
- polynucleotide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000000855 fermentation Methods 0.000 title claims abstract description 48
- 230000004151 fermentation Effects 0.000 title claims abstract description 48
- 239000004472 Lysine Substances 0.000 title claims abstract description 13
- 238000002360 preparation method Methods 0.000 title claims description 11
- 102000000818 NADP Transhydrogenases Human genes 0.000 claims abstract description 73
- 108010001609 NADP Transhydrogenases Proteins 0.000 claims abstract description 73
- 241000894006 Bacteria Species 0.000 claims abstract description 40
- 108091033319 polynucleotide Proteins 0.000 claims abstract description 28
- 102000040430 polynucleotide Human genes 0.000 claims abstract description 28
- 239000002157 polynucleotide Substances 0.000 claims abstract description 28
- 239000002245 particle Substances 0.000 claims description 26
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 claims description 24
- 229930195722 L-methionine Natural products 0.000 claims description 24
- 150000001413 amino acids Chemical group 0.000 claims description 14
- 239000000654 additive Substances 0.000 claims description 12
- 239000013612 plasmid Substances 0.000 claims description 12
- 239000002773 nucleotide Substances 0.000 claims description 11
- 125000003729 nucleotide group Chemical group 0.000 claims description 11
- 241000186031 Corynebacteriaceae Species 0.000 claims description 10
- 238000001035 drying Methods 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 9
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 claims description 8
- 238000005507 spraying Methods 0.000 claims description 8
- 230000008676 import Effects 0.000 claims description 7
- 239000004459 forage Substances 0.000 claims description 6
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims description 5
- 238000012216 screening Methods 0.000 claims description 5
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 claims description 4
- 239000001888 Peptone Substances 0.000 claims description 4
- 108010080698 Peptones Proteins 0.000 claims description 4
- 229960000304 folic acid Drugs 0.000 claims description 4
- 235000019152 folic acid Nutrition 0.000 claims description 4
- 239000011724 folic acid Substances 0.000 claims description 4
- 239000012528 membrane Substances 0.000 claims description 4
- 235000019319 peptone Nutrition 0.000 claims description 4
- XUYPXLNMDZIRQH-LURJTMIESA-N N-acetyl-L-methionine Chemical compound CSCC[C@@H](C(O)=O)NC(C)=O XUYPXLNMDZIRQH-LURJTMIESA-N 0.000 claims description 3
- 229930182817 methionine Natural products 0.000 claims description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 2
- 229930003756 Vitamin B7 Natural products 0.000 claims description 2
- 238000012258 culturing Methods 0.000 claims description 2
- 239000011735 vitamin B7 Substances 0.000 claims description 2
- 235000011912 vitamin B7 Nutrition 0.000 claims description 2
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 abstract description 16
- 238000000034 method Methods 0.000 abstract description 11
- 239000000047 product Substances 0.000 abstract description 10
- 235000019766 L-Lysine Nutrition 0.000 abstract description 6
- 239000013067 intermediate product Substances 0.000 abstract description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 230000000694 effects Effects 0.000 description 12
- 108090000623 proteins and genes Proteins 0.000 description 9
- 102000004190 Enzymes Human genes 0.000 description 8
- 108090000790 Enzymes Proteins 0.000 description 8
- 235000001014 amino acid Nutrition 0.000 description 6
- 230000001580 bacterial effect Effects 0.000 description 5
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 4
- 230000000996 additive effect Effects 0.000 description 4
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- 238000006243 chemical reaction Methods 0.000 description 4
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- 230000000968 intestinal effect Effects 0.000 description 4
- 235000018977 lysine Nutrition 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
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- 150000007523 nucleic acids Chemical group 0.000 description 3
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 2
- 108020004705 Codon Proteins 0.000 description 2
- 102000012410 DNA Ligases Human genes 0.000 description 2
- 108010061982 DNA Ligases Proteins 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 2
- 101000702488 Rattus norvegicus High affinity cationic amino acid transporter 1 Proteins 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 238000000246 agarose gel electrophoresis Methods 0.000 description 2
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- 238000010367 cloning Methods 0.000 description 2
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- 238000006062 fragmentation reaction Methods 0.000 description 2
- 235000011187 glycerol Nutrition 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 238000010369 molecular cloning Methods 0.000 description 2
- 150000003222 pyridines Chemical class 0.000 description 2
- 238000011160 research Methods 0.000 description 2
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- 229930003231 vitamin Natural products 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 150000003722 vitamin derivatives Chemical class 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000186226 Corynebacterium glutamicum Species 0.000 description 1
- 229920001353 Dextrin Polymers 0.000 description 1
- 239000004375 Dextrin Substances 0.000 description 1
- 239000004278 EU approved seasoning Substances 0.000 description 1
- 108010042407 Endonucleases Proteins 0.000 description 1
- 102000004533 Endonucleases Human genes 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 108010021757 Polynucleotide 5'-Hydroxyl-Kinase Proteins 0.000 description 1
- 102000008422 Polynucleotide 5'-hydroxyl-kinase Human genes 0.000 description 1
- 241000607720 Serratia Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
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- 238000005516 engineering process Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000011194 food seasoning agent Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
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- 244000005700 microbiome Species 0.000 description 1
- 210000001589 microsome Anatomy 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- -1 pH7.3 Substances 0.000 description 1
- 238000004816 paper chromatography Methods 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 230000026731 phosphorylation Effects 0.000 description 1
- 238000006366 phosphorylation reaction Methods 0.000 description 1
- 239000013600 plasmid vector Substances 0.000 description 1
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- 230000035939 shock Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
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Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
Claims (11)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN2011100656943A CN102191288B (zh) | 2011-03-18 | 2011-03-18 | 赖氨酸的反式发酵制备 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN2011100656943A CN102191288B (zh) | 2011-03-18 | 2011-03-18 | 赖氨酸的反式发酵制备 |
Publications (2)
Publication Number | Publication Date |
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CN102191288A CN102191288A (zh) | 2011-09-21 |
CN102191288B true CN102191288B (zh) | 2012-08-15 |
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Family Applications (1)
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CN2011100656943A Active CN102191288B (zh) | 2011-03-18 | 2011-03-18 | 赖氨酸的反式发酵制备 |
Country Status (1)
Country | Link |
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CN (1) | CN102191288B (zh) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102763771B (zh) * | 2012-06-07 | 2013-10-16 | 宁夏伊品生物科技股份有限公司 | 赖氨酸、木聚糖酶的发酵及包被 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1139956C (zh) * | 1999-05-24 | 2004-02-25 | 阿尔卑斯电气株式会社 | 按钮开关 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE4130868C2 (de) * | 1991-09-17 | 1994-10-13 | Degussa | Tierfuttermittelsupplement auf der Basis einer Aminosäure und Verfahren zu dessen Herstellung |
US5830716A (en) * | 1993-10-28 | 1998-11-03 | Ajinomoto Co., Inc. | Increased amounts of substances by modifying a microorganism to increase production of NADPH from NADH |
KR101052573B1 (ko) * | 2004-04-02 | 2011-07-29 | 씨제이제일제당 (주) | 균일한 함량을 갖는 과립형 동물 사료 첨가제를 제조하는 방법 및 그에 의하여 제조되는 과립형 동물 사료 첨가제 |
US20070082031A1 (en) * | 2005-10-08 | 2007-04-12 | Hermann Lotter | L-lysine-containing feed additives |
-
2011
- 2011-03-18 CN CN2011100656943A patent/CN102191288B/zh active Active
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1139956C (zh) * | 1999-05-24 | 2004-02-25 | 阿尔卑斯电气株式会社 | 按钮开关 |
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CN102191288A (zh) | 2011-09-21 |
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GR01 | Patent grant | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Trans-lysine fermentation preparation Effective date of registration: 20190201 Granted publication date: 20120815 Pledgee: Yongning County Branch of China Agricultural Development Bank Pledgor: NINGXIA EPPEN BIOTECH Co.,Ltd.|INNER MONGOLIA EPPEN BIOTECH Co.,Ltd. Registration number: 2019640000001 |
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Date of cancellation: 20200116 Granted publication date: 20120815 Pledgee: Yongning County Branch of China Agricultural Development Bank Pledgor: NINGXIA EPPEN BIOTECH Co.,Ltd. Registration number: 2019640000001 |
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PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Trans-lysine fermentation preparation Effective date of registration: 20200120 Granted publication date: 20120815 Pledgee: Yongning County Branch of China Agricultural Development Bank Pledgor: NINGXIA EPPEN BIOTECH Co.,Ltd. Registration number: Y2020640000002 |
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PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
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Date of cancellation: 20210126 Granted publication date: 20120815 Pledgee: Yongning County Branch of China Agricultural Development Bank Pledgor: NINGXIA EPPEN BIOTECH Co.,Ltd. Registration number: Y2020640000002 |
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PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Preparation of lysine by trans fermentation Effective date of registration: 20210127 Granted publication date: 20120815 Pledgee: Yongning County Branch of China Agricultural Development Bank Pledgor: NINGXIA EPPEN BIOTECH Co.,Ltd. Registration number: Y2021640000001 |
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Date of cancellation: 20220129 Granted publication date: 20120815 Pledgee: Yongning County Branch of China Agricultural Development Bank Pledgor: NINGXIA EPPEN BIOTECH Co.,Ltd. Registration number: Y2021640000001 |
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PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Preparation of lysine by trans fermentation Effective date of registration: 20220323 Granted publication date: 20120815 Pledgee: Yongning County Branch of China Agricultural Development Bank Pledgor: NINGXIA EPPEN BIOTECH Co.,Ltd. Registration number: Y2022640000002 |
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Date of cancellation: 20230315 Granted publication date: 20120815 Pledgee: Yongning County Branch of China Agricultural Development Bank Pledgor: NINGXIA EPPEN BIOTECH Co.,Ltd.|INNER MONGOLIA EPPEN BIOTECH Co.,Ltd.|Heilongjiang Yipin Biotechnology Co.,Ltd. Registration number: Y2022640000002 |
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PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Preparation of Lysine by Trans Fermentation Granted publication date: 20120815 Pledgee: Yongning County Branch of China Agricultural Development Bank Pledgor: NINGXIA EPPEN BIOTECH Co.,Ltd.|INNER MONGOLIA EPPEN BIOTECH Co.,Ltd.|Heilongjiang Yipin Biotechnology Co.,Ltd. Registration number: Y2024640000005 |
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