CN102188681B - Traditional Chinese drug composition for treating pelvic inflammatory disease, preparation method, application thereof - Google Patents
Traditional Chinese drug composition for treating pelvic inflammatory disease, preparation method, application thereof Download PDFInfo
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Abstract
The invention discloses a traditional Chinese drug composition for treating pelvic inflammatory disease (PID). The traditional Chinese drug composition is prepared from the following raw materials, by weight, 10-20 parts of blue turmeric rhizome, 15-25 parts of Chinese angelica, 6-10 parts of safflower, 20-40 parts of common motherwort herb, 6-10 parts of yanhusuo tuber, 6-10 parts of combined spicebush root, 10-20 parts of aahesive rehmannia root tuber, 10-20 parts of giant knotweed rhizome, 10-20 parts of common knotweed herb, 10-20 parts of asiatic plantain seed. The invention further discloses a preparation method for preparing the traditional Chinese drug composition, and an application of the traditional Chinese drug composition. The traditional Chinese drug composition provided by the present invention has advantages of activating blood and dissolving stasis, clearing heat and removing dampness, and can be used for treating the PID.
Description
Technical field
The invention belongs to technical field of Chinese medicines, be specifically related to a kind of Chinese medicine composition that is used to treat pelvic inflammatory disease and preparation method thereof and use.
Background technology
Pelvic inflammatory disease is meant the inflammatory damage of reproductive tract on the women and proximity structure thereof, and acute pelvic inflammatory disease is lost and controlled, the wrong treatment delay forms chronic pelvic inflammatory disease.Pelvic inflammatory disease is a kind of commonly encountered diseases, the frequently-occurring disease of gynecological clinic, and relapse rate is high.In the U.S., annual nearly 1,000,000 pelvic inflammatory disease patients, wherein about 250,000 women are because of the pelvic inflammatory disease hospitalization, and about 1/4th pelvic inflammatory disease patient shows effect repeatedly, forms chronic pelvic inflammatory disease.The sickness rate of domestic pelvic inflammatory disease has not been seen particulars report, but owing to sexual behaviour is open, the increasing of sexually transmitted disease (STD), sexual life age in advance, the reasons such as confusion of property mode, cause its sickness rate to increase year by year.The incidence rate that pelvic inflammatory disease infects the back infertility first is 11%, and the incidence rate of superinfection infertility rises to 50%, and the prompting pelvic inflammatory disease is the major reason that causes infertility.
The western medical treatment of taking to this disease is at present mainly started with from two aspects: promptly uses antibiotic therapy, is intended to eliminate the etiological treatment of pathogenic microorganism, but for chronic pelvic inflammation; Because tissue adhesionization, local circulation obstacle; Antibiotic is difficult to infiltrate the part and plays a role, and is relatively poor to fibrous tissue and the connective tissue effect of eliminating inflammatory infiltration, and antibiotic does not possess the adhesion of alleviation and analgesic effect; So chronic pelvic inflammatory disease is used antibiotic therapy merely, curative effect is not fully up to expectations; Be exactly to use hormone, Chymetin, placenta interstitial fluid symptomatic treatment in addition, certain limitation and toxic and side effects are all arranged with the reaction that reduces inflammation, fibrosis formation and granulation tissue hyperplasia, enhancing human body immunity power.The Chinese patent medicine of the traditional Chinese medical science aspect the treatment pelvic inflammatory disease is a lot, but curative effect is unstable mostly, and cure rate is low, is prone to recurrence.The present invention adopts blood circulation promoting and blood stasis dispelling, and the method treatment pelvic inflammatory disease of clearing away heat and eliminating dampness has obtained significant curative effect.
Summary of the invention
Technical problem to be solved by this invention provides a kind of Chinese medicine composition that is used to treat pelvic inflammatory disease, and its therapeutic effect is obvious, and has no side effect.
The technical problem that the present invention also will solve provides the method for preparing of above-mentioned Chinese medicine composition.
The last technical issues that need to address of the present invention provide the application of above-mentioned Chinese medicine composition in preparation treatment pelvic inflammatory disease medicine.
In order to solve the problems of the technologies described above, the technical scheme that the present invention adopts is following:
A kind of Chinese medicine composition that is used to treat pelvic inflammatory disease, it is obtained by the feedstock production of following parts by weight: Rhizoma Curcumae 10-20 part, Radix Angelicae Sinensis 15-25 part; Flos Carthami 6-10 part; Herba Leonuri 20-40 part, Rhizoma Corydalis 6-10 part, Radix Linderae 6-10 part; Radix Rehmanniae 10-20 part; Rhizoma Polygoni Cuspidati 10-20 part,
holds 10-20 part, Semen Plantaginis 10-20 part.
The above-mentioned Chinese medicine composition that is used to treat pelvic inflammatory disease, preferably the feedstock production by following parts by weight obtains: 15 parts of Rhizoma Curcumae, 20 parts of Radix Angelicae Sinensis; 8 parts on Flos Carthami; 30 parts of Herba Leonuris, 8 parts of Rhizoma Corydalis, 8 parts of the Radixs Linderae; 15 parts of Radix Rehmanniae; 15 parts of Rhizoma Polygoni Cuspidati,
holds 15 parts, 15 parts of Semen Plantaginiss.
The above-mentioned Chinese medicine composition that is used to treat pelvic inflammatory disease, described Radix Rehmanniae is preferably Radix Rehmanniae.
The above-mentioned Chinese medicine composition that is used to treat pelvic inflammatory disease; Said Chinese medicine composition adds adjuvant, processes capsule, tablet, granule, pill, pellet, soft capsule, drop pill, oral liquid, dispersible tablet, effervescent tablet, chewable tablet or suppository with conventional formulation method.
The above-mentioned method for preparing that is used to treat the Chinese medicine composition of pelvic inflammatory disease, it comprises the steps:
(1) Rhizoma Curcumae, Radix Angelicae Sinensis two flavor medical materials are through supercritical CO
2After the extraction, obtain extract and medicinal residues, medicinal residues are subsequent use, and the extract by adopting saturated water solution method gets Benexate Hydrochloride with beta-cyclodextrin inclusion compound, cold preservation, sucking filtration, drying;
(2) supercritical CO in the step (1)
2Medicinal residues that obtain after the extraction and Flos Carthami, Herba Leonuri, Rhizoma Corydalis, the Radix Linderae, Radix Rehmanniae, Rhizoma Polygoni Cuspidati,
Hold and Semen Plantaginis 8 flavor medical materials merging, decocte with water twice, collecting decoction, relative density is 1.10-1.20 when being concentrated into 65 ℃; Add ethanol and make the alcohol amount of containing reach 65-85% (v/v), stir, leave standstill; Filter, relative density was 1.20-1.30 and reclaims ethanol, the concentrated solution vacuum drying when filtrate decompression was concentrated into 65 ℃; Add the Benexate Hydrochloride that step (1) obtains, pulverize, mix homogeneously promptly gets.
In the step (1), supercritical CO
2Extraction conditions is: extraction temperature 20-60 ℃, pressure 20-40MPa, extraction time 1-3h, entrainer are 95% (v/v) ethanol.
In the step (1), the beta-cyclodextrin inclusion compound condition is: 30-70 ℃ following continuous stirring 3-5 hour, speed of agitator is 80-120 rev/min; Cold preservation time is 24-48 hour; Baking temperature is 40-50 ℃.
In the step (2), decocting condition is: the 8-12 that adds for the first time water and be medical material weight doubly measures, and decocts 1-2h, and the 6-10 that adds water for the second time and be medical material weight doubly measures, and decocts 1-2h.
In the step (2), concentrated condition optimization is 0.07MPa, 60-70 ℃ following concentrating under reduced pressure.
After step (2) finishes, add adjuvant and process capsule, tablet, granule, pill, pellet, soft capsule, drop pill, dispersible tablet, effervescent tablet, chewable tablet or suppository, or with in the step (2); Directly add the Benexate Hydrochloride that step (1) obtains in the concentrated solution after ethanol reclaims; Mix homogeneously filters packing; Oral liquid is processed in sterilization.
The above-mentioned Chinese medicine composition that is used for treating pelvic inflammatory disease is in the application for preparing treatment pelvic inflammatory disease medicine.
Rhizoma Curcumae is monocotyledon Zingiberaceae Zingiberaceae Rhizoma Curcumae Curcuma phaeocaulis Val. among the present invention; Or RADIX CURCUMAE (claiming warm Rhizoma Curcumae again) C.wenyujin Y.H.Chen et C.Ling, the dry rhizome of Guangxi zedoary (claiming the osmanthus Rhizoma Curcumae again) C.kwangsiensis S.lee et C.F.Liang.Its property toil temperature is nontoxic, goes into liver, spleen channel, has the circulation of qi promoting removing blood stasis, and the effect of removing food stagnancy pain relieving is controlled trusted subordinate's distending pain, lumps in the chest and abdomen, women's blood stasis amenorrhea." the article remittance is smart to be wanted ": " removing mass gathers "." the SHENGCAO property of medicine is wanted fully ": " applying the skin ulcer detumescence, eliminating stasis to stop pain ".Modern study find this medicine can antitumor, antiearly pregnancy, antiinflammatory, antibiotic, effect such as enzyme falls.Also has antiinflammatory action in addition; It is reported that peritonitis that mouse gavaging Rhizoma Curcumae volatile oil 200mg/kg Dichlorodiphenyl Acetate produces has the inhibitory action of highly significant; Mouse peritoneal injection Rhizoma Curcumae volatile oil 200mg/kg has obvious inhibitory action to scald property local edema; Lumbar injection 100m/kg has obvious inhibitory action to ear's inflammation that Oleum Tiglii causes, rats by intraperitoneal injection volatile oil 75mg/kg/ days had obvious inhibitory action to subcutaneous granuloma induced by implantation of cotton pellets hypertrophy after 9 days.
When the dry root that is classified as umbelliferae angelica Angelica sinensis (Oliv.) Diels.Autumn end excavates, and removes fibrous root and silt, treat that moisture evaporates slightly after, tie into wisp, last canopy is with pyrotechnics smoke-dried beancurd slowly.It is warm in nature, hides sweet, hot.Return liver, the heart, spleen channel.Can enrich blood and invigorate blood circulation, menstruction regulating and pain relieving, loosening bowel to relieve constipation.Be used for blood deficiency and yellow complexion, dizzy cardiopalmus, menoxenia, amenorrhea dysmenorrhea, asthenia cold abdominalgia, dryness of the intestine constipation, rheumatic arthralgia, injury from falling down, ulcer sores.Also has antiinflammatory action according to another report.
Flos Carthami is the tubular corolla of feverfew Flos Carthami Carthamus tinctorius L..Main product in Henan, Hubei, Sichuan, Yunnan, zhejiang and other places.The summer harvest is bloomed, and pattern is transferred to when scarlet by Huang to be plucked.Dry in the shade or the low baking temperature oven dry.Warm in nature, acrid in the mouth.Can invigorate blood circulation latus rectum, eliminating stasis to stop pain.Be used for that amenorrhea, dysmenorrhea, lochia are not all right, lump in the abdomen mass in the abdomen, traumatic injury.
Herba Leonuri is the young stem and leaf of labiate Herba Leonuri Leonurus heterophyllus Sweet..The Herba Leonuri young stem and leaf contains multiple nutritional components such as protein, carbohydrate.Nature and flavor are arduous cool, have invigorate blood circulation, the effect of blood stasis dispelling, regulating menstruation, the water that disappears.Control water, hematuria under menoxenia, the edema, rush down blood, dysentery, haemorrhoids.Herba Leonuri also has the effects such as cellular immunization of enhancing body in addition.
Rhizoma Corydalis is the dry tuber of papaveraceae plant corydalis Corydalis yanhusuo W.T.Wang.Excavate when the early summer stem and leaf is withered, remove fibrous root, clean, put and boil in the boiling water, take out, dry to proper no Bai Xinshi.The property hot, bitter, temperature.Return liver, spleen channel.Can invigorate blood circulation promoting the circulation of QI, pain relieving.Be used for the breast side of body, epigastric pain, amenorrhea dysmenorrhea, postpartum stagnation, tumbling and swelling.Pharmacological action shows that it has: 1. analgesic activity: the Rhizoma Corydalis powder has analgesic activity, and its pain relieving is tired and is about 1/100 of opium; Rhizoma Corydalis total alkaloids, Rhizoma Corydalis first element, plain, the ugly element of second, the last of the ten Heavenly stems, element all had analgesic activity, and is stronger with the second element.Various dosage forms are strong with alcohol system extractum and powder effect.2. calm, stable, syngignoscism: tetrahydropalmatine has certain calmness, stable effect, and its levo form is novel central inhibitor, when heavy dose of rabbit, Canis familiaris L., monkey is had tangible syngignoscism.3. to the influence of digestive system: dehydrogenation Rhizoma Corydalis first have the experimental gastric ulcer effect of significant Chinese People's Anti-Japanese Military and Political College Mus.4. to cardiovascular effect: tetrahydropalmatine, uglyly have slight blood pressure lowering, a decreased heart rate effect; Dehydrocory daline has blood pressure lowering, increases the CBSF effect.The downright bad effect of rat heart muscle that ethanol extract also has coronary artery dilating, antagonism isoproterenol to cause.
The Radix Linderae is the tuber of canella Radix Linderae Lindera aggregata (Sims) Kosterm..Main product in Zhejiang, ground such as Anhui, Jiangsu, Shaanxi.All can excavate the whole year, removes radicula, cleans, and takes advantage of fresh slices, dries.Give birth to and use or parched with bran usefulness.The property hot, temperature.Return lung, spleen, kidney, urinary bladder channel.The ability promoting the circulation of QI to relieve pain, warming kidney for dispelling cold.The Radix Linderae has pharmacologically active comparatively widely; Mainly as follows: 1, antibiotic, antivirus action: reports such as Zhang Tianmin; The medicinal liquid of the Radix Linderae 20% has the obvious suppression effect to respiratory syncytial virus (RSV), Ke Saji B1, B3, B4 virus (CBV); RSV virus be the main diseases that causes baby's bronchiolitis and pneumonia because of, the inhibition index of Ke Saji B papova (CBV) is 4 logarithms, belongs to high-efficient antiviral medicament.The water of the Radix Linderae and alcohol extract also have the obvious suppression effect to herpes simplex virus (HSV), also belong to efficient medicine.The Radix Linderae all has inhibitory action to staphylococcus aureus, first type Streptococcus hemolyticus, Bacillus typhi, Bacillus proteus, bacillus pyocyaneus, escherichia coli.In addition, bright Folium Linderae strychnifoliae also has antibacterial action.
Radix Rehmanniae is the fresh or dried root of scrophulariaceae rehmannia glutinosa plant Rehmannia glutinosa Libosch..Excavate autumn, removes removing LU, fibrous root and silt, using fresh herb; Or Radix Rehmanniae slowly cured to about eighty per cant do.The former practises title " Radix Rehmanniae ", and the latter practises title " Radix Rehmanniae ".Radix Rehmanniae property is sweet, and is cold.GUIXIN, liver, kidney channel.Clearing away heat and cooling blood, yin nourishing is promoted the production of body fluid.Be used for calentura crimson tongue excessive thirst, interior-heat caused by deficiency of YIN, the hectic fever due to YIN-deficiency consumptive fever, interior-heat is quenched one's thirst, and spits blood, and epistaxis is sent out the speckle dermexanthesis.It has antiinflammatory, anti-allergic effects: irritating stomach like report Radix Rehmanniae decoct has tangible antagonism to rat formaldehyde property arthritis and albumen arthritis; And can suppress the increase of the capillary permeability that granuloma that the Oleum Terebinthinae subcutaneous injection causes and histamine cause. the Radix Rehmanniae aqueous extract has obvious inhibitory action to the mouse peritoneum inflammation that vascular permeability increases and acetic acid causes that histamine causes; Acute inflammation due to the Ovum Gallus domesticus album is also had antiinflammatory action, and alcohol of Radix Rehmanniae and ether extracting solution then do not have the antiinflammatory effect.Have report to think that the Radix Rehmanniae water extract can significantly increase the T lymphocyte in the peripheral blood, the Radix Rehmanniae alcohol extract can obviously promote anti-SRPC antibody-hemolysin to generate, and reduces peripheral blood T lymphocyte, and the Radix Rehmanniae Preparata water extract does not then have this effect.Also have antifungic action: Radix Rehmanniae water logging agent has inhibitory action to multiple fungi growth such as trichophyton mentagrophytes, Gypsum Fibrosum appearance microspore tinea bacterium, ulotrichy microspore's tinea bacterium and Du's big belly microspore tinea bacterium difficult to understand.
Rhizoma Polygoni Cuspidati is Polygonaceae Polygonaceae plant polygonum cuspidatum Polygonum cuspidatum Sieb.et Zucc., dry rhizome and root.Spring, Qiu Erji excavate, and remove fibrous root, clean, and take advantage of short section of fresh-cut or sheet, dry.Put dry place, mildew-resistant, mothproof.The little hardship of property is slightly cold.The ability heat-clearing and toxic substances removing, promoting the function of the gallbladder to alleviate jaundice, expelling wind and removing dampness, dissipating blood stasis analgesic therapy, relieving cough and resolving phlegm.Be used for arthralgia, jaundice due to damp-heat, amenorrhea ， mass in the abdomen, cough with copious phlegm, burn due to hot liquid or fire, injury from falling down, carbuncle sore tumefacting virus.Its emodin that contains, 7-acetyl group-2-methoxyl group-6-methyl-8-hydroxyl-1, the 4-naphthoquinone has antibacterial activity.The test of the resveratrol in-vitro antibacterial that contains shows: it has powerful anti-microbial property to trichophyton rubrum, the trichophyton interdigitalis that causes stubborn dermatitis, pompholyx shape tinea alba, and to bacillus subtilis, Resina garciniae eight repeatedly bacterium etc. stronger bactericidal action is arranged.Emodin, emodin-8-glycoside have inhibitory action to staphylococcus aureus, hepatitis diplococcus.A kind of Flavonoid substances that contains in the Rhizoma Polygoni Cuspidati has inhibitory action to staphylococcus aureus, Staphylococcus albus, Bacillus proteus.But 10% Rhizoma Polygoni Cuspidati decocting liquid all has inhibitory action to herpes simplex virus, the influenza Asia first type capital 68-1 of section virus and dust II type virus (ECHO11).3% decocting liquid to No. 479 adenovirus type IIIs, No. 72 poliomyelitis II types, No. 44 dusts can 9 types, COxsackie A9 type and B5 type, encephalitis B (Jing Wei grinds the I strain), No. 140 7 kinds of representative Strain such as herpes simplex all have stronger inhibitory action.Rhizoma Polygoni Cuspidati monomer I and II can make the hepatitis B antigen titre reduce by 8 times.
holds the dry aerial parts for Polygonaceae annual herb plant Herba Polygoni Avicularis Polygonum aviculare L..Herba Polygoni Avicularis in all parts of the country all produces.Gather summer, dries, and chopping is given birth to and used leaf alternate, lanceolar, all herbal medicine.The property bitter, be slightly cold.Return urinary bladder channel.These article hardship is fallen row, the tonneau bladder, bitter dry again can the parasite killing removing damp to relieve itching, be mainly used in and drench pain and eczema.The ability relieving stranguria by diuresis is used for damp-heat gonorrhoea, can treat urinary system infection, calculus, hematuria etc.The ability killing parasites for relieving itching can be treated parasitic disease such as pinworm.Also can fry in shallow oil soup washout treatment skin skin ulcer rash, pruritus.Pharmacological action: 1. diuresis: after Herba Polygoni Avicularis decoct 20g/kg gave the rat of saline load, urine amount, sodium, potassium was discharged all increases, and particularly the discharge of potassium is more, and its ash also has effect same.Have report to think, its diuresis mainly is owing to due to the potassium salt, also have report to think because wherein due to the contained flavonoid glycoside.2. antibacterial action: in vitro, Herba Polygoni Avicularis has inhibitory action to trichophyton mentagrophytes, ulotrichy microspore bacterium; To the bacteriostasis of dysentery bacterium a little less than, can suppress the growth of bacterium flexneri VI type and bacterium sonnei during 25% concentration; Inhibitory action to other dysentery bacteriums is not obvious.But clinical therapeutic efficacy is still satisfactory, does not conform to its fungistatic effect in test tube.3. other effects: avacularin has choleretic effect to rat, dog.The water of Herba Polygoni Avicularis and alcohol extract can accelerate blood solidify, and uterus tension force is increased, and can be used as the hemorrhage of miscarriage and childbirth metrorrhagia; Give cat, rabbit, Canis familiaris L. intravenous injection, hypotensive effect is arranged.
Semen Plantaginis is the dry mature seed of Plantaginaceae plant Herba Plantaginis.All be wild.The fruit ear of gathering during seed maturity in summer, two season of autumn dries, and rubs seed, removes impurity.Main product in Heilungkiang, ground such as Liaoning, Hebei.Nature and flavor are sweet; Light; Cold nature.Return lung; Liver; Kidney; Urinary bladder channel.The ability clearing away heat and promoting diuresis; Eliminating dampness by diuresis antidiarrheal; Make eye bright; Eliminate the phlegm.Main dysuria; The stranguria with turbid discharge leukorrhagia; The edema distension; The heat-damp in summer dysentery; Conjunctival congestion is hidden from view; The expectorant heat syndrome cough is breathed heavily.Pharmacological action: 1. diuresis: the normal person is taken Semen Plantaginis decoct 10g orally, and diuresis is arranged.Zoopery shows that Semen Plantaginis can make moisture, sodium chloride, carbamide and uric acid discharge increase and diuresis is arranged.But also have report to point out, normal person and rabbit clothes Semen Plantaginis decoct do not have obvious diuresis, and the Plantago depressa Willd seed does not also have diuresis.2. other effects: the Semen Plantaginis water decoction has laxative action to rat and Canis familiaris L..The feed cholesterol is made it the rabbit that content raises in the serum, and the Plantago ovata seed oil can make its cholesterol level descend rapidly; But should normal rabbits there be the effect that reduces serum cholesterol level by oil.Experiment shows, 5% Semen Plantaginis liquid, 0.05～0.2ml gives rabbit knee joint intracavitary administration (continuous 2～4 times, each 3～14 days at interval), and the rabbit of impelling membrana synovialis capsulae articularis connective tissue proliferation effect is arranged, thereby makes the joint capsule that has relaxed recover original tensity.Have report to think, the Semen Plantaginis alcoholic extract has the effect of the choline of plan, can reduce the blood pressure of anesthetized dog, cat, suppresses extrasomatic rabbit heart, the frog heart, the intestinal tube of excited rabbit, rat and Cavia porcellus, and can be suppressed by atropine.
The traditional Chinese medical science think pelvic inflammatory disease many by through row, puerperal vessels of the uterus hollow, the evil invasion of damp and hot poison, blood stasis congelation injures to appoint is with two arteries and veins, or the stagnation of QI due to depression of the liver blood stasis, overstrain causes dysfunction of the spleen in transportation, retention of water-damp, strongly fragrant fire-transformation of a specified duration and cause retention of damp-heat in the interior.Rhizoma Curcumae, Radix Angelicae Sinensis, Flos Carthami and Herba Leonuri blood circulation promoting and blood stasis dispelling are monarch drug among the present invention; Rhizoma Corydalis and Radix Linderae promoting the circulation of QI to relieve pain, the power that principal drug assistance is invigorated blood circulation makes QI and blood capable and not stagnant; Radix Rehmanniae, Rhizoma Polygoni Cuspidati,
hold and the Semen Plantaginis clearing away heat and cooling blood; The diuresis eliminating dampness by diuresis is an adjuvant, and all medicines share, and full side plays blood circulation promoting and blood stasis dispelling altogether; The clearing away heat and eliminating dampness effect can be used for treating pelvic inflammatory disease.
Beneficial effect: the present invention compared with prior art has following advantage
1, we are to the pathogenesis of pelvic inflammatory disease, and dialectical rationally compatibility is accurate, and adopts blood circulation promoting and blood stasis dispelling, and clearing away heat and eliminating dampness is a principle of treatment, obtains good result.
2, be directed against the effective ingredient characteristics of different medical materials among the preparation technology, adopt different extraction processes, reasonable in design, keep more effective ingredient.
3, crude drug is pharmacopeia and records kind in this prescription, and Ji Yuan is clear and definite, and side effect is little, and determined curative effect is applicable to long-term prescription.
4, still do not have ideal specific treatment medicine for pelvic inflammatory disease both at home and abroad at present, the existing clinically big multiaction of medicine of preventing and treating pelvic inflammatory disease commonly used relaxes the therapeutic domain limitation; Curative effect is not remarkable; The medicine life-time service that has has damage to liver, should not be used for the treatment of pelvic inflammatory disease, and the medicine that has also can produce toxic and side effects; For this reason, need develop the needs that more medicines of treating senile vaginitis safely and effectively satisfy clinical treatment.Prevalence is high, and the relapse rate height is the main feature of pelvic inflammatory disease, reflects that simultaneously the demand in pelvic inflammatory disease medicine market is huge.This subject study can actively prevent and treat the generation and the development of pelvic inflammatory disease effectively, and this is for the health of the existence that improves numerous women with quality of life, the numerous women of protection, and then the raising productivity, significant and higher utility.Because the primary disease sickness rate is high, estimate to use once marketing, will produce good social benefit and economic benefit.
The specific embodiment
Below through the embodiment form; Foregoing of the present invention is remake further detailed description; But should this be interpreted as that the scope of the above-mentioned theme of the present invention only limits to following instance, all technology that realizes based on foregoing of the present invention all belong to scope of the present invention.
Each embodiment needed raw material parts by weight of table 1, unit: part
Among the embodiment; Rhizoma Curcumae is monocotyledon Zingiberaceae Zingiberaceae Rhizoma Curcumae Curcuma phaeocaulisVal.; Or RADIX CURCUMAE (claiming warm Rhizoma Curcumae again) C.wenyujin Y.H.Chen et C.Ling, the dry rhizome of Guangxi zedoary (claiming the osmanthus Rhizoma Curcumae again) C.kwangsiensis S.lee et C.F.Liang.When the dry root that is classified as umbelliferae angelica Angelicasinensis (Oliv.) Diels.Flos Carthami is the tubular corolla of feverfew Flos Carthami Carthamus tinctorius L..Herba Leonuri is the young stem and leaf of labiate Herba Leonuri Leonurus heterophyllus Sweet..Rhizoma Corydalis is the dry tuber of papaveraceae plant corydalis Corydalis yanhusuo W.T.Wang.The Radix Linderae is the tuber of canella Radix Linderae Linderaaggregata (Sims) Kosterm..Radix Rehmanniae is the fresh or dried root of scrophulariaceae rehmannia glutinosa plant Rehmannia glutinosa Libosch., and these article are Radix Rehmanniae.Rhizoma Polygoni Cuspidati is Polygonaceae Polygonaceae plant polygonum cuspidatum Polygonumcuspidatum Sieb.et Zucc., dry rhizome and root.
holds the dry aerial parts for Polygonaceae annual herb plant Herba Polygoni Avicularis Polygonumaviculare L..Semen Plantaginis is the dry mature seed of Plantaginaceae plant Herba Plantaginis.
Embodiment 1: the preparation of capsule.
Press the preparation of table 1 proportioning raw materials and capsule dosage form conventional method, be prepared into capsule, concrete grammar is following:
Get 10 parts of Rhizoma Curcumae, 25 part of two flavor of Radix Angelicae Sinensis medical material, adopt supercritical CO
2Extract 1h, 20 ℃ of extraction temperature, pressure 20MPa, entrainer are 95% ethanol; Get supercritical extract, adopt saturated water solution method with beta-cyclodextrin inclusion compound, continuous stirring is 3 hours under 30 ℃ of heat-retaining conditions; Speed of agitator is 80 rev/mins, cold preservation 24 hours, sucking filtration; 40 ℃ of dryings get Benexate Hydrochloride, and other deposits;
Above-mentioned supercritical CO
26 parts on medicinal residues behind the leaching process and Flos Carthami, 40 parts of Herba Leonuris, 6 parts of Rhizoma Corydalis, 10 parts of the Radixs Linderae, 10 parts of Radix Rehmanniae, 20 parts of Rhizoma Polygoni Cuspidati,
Hold 10 parts and the merging of 20 part of 8 flavor of Semen Plantaginis medical material, decocte with water twice adds 8 times of amounts that water is medical material weight for the first time, decocts 1h; Add for the second time 6 times of amounts that water is medical material weight, decoct 1h, collecting decoction, relative density is 1.10 when being evaporated to 65 ℃ under 0.07MPa, 60-70 ℃; Add 95% (v/v) ethanol and make the alcohol amount of containing reach 65% (v/v), stir, left standstill 24 hours, decompression filtrate recycling ethanol also concentrates; Relative density was 1.20 when filtrating became to 65 ℃ at concentrating under reduced pressure under 0.07MPa, the 60 ℃ of conditions, and vacuum drying adds above-mentioned Benexate Hydrochloride, pulverized; Add starch etc., dry granulation is crossed 60 mesh sieves, No. 1 capsule of packing into get final product capsule.
Embodiment 2: the preparation of granule.
Press the preparation of table 1 proportioning raw materials and granule dosage form conventional method, be prepared into granule, concrete grammar is following:
Get 15 parts of Rhizoma Curcumae, 20 part of two flavor of Radix Angelicae Sinensis medical material, adopt supercritical CO
2Extract 2h, 40 ℃ of extraction temperature, pressure 30MPa, entrainer are 95% ethanol; Get supercritical extract, adopt saturated water solution method with beta-cyclodextrin inclusion compound, continuous stirring is 4 hours under 50 ℃ of heat-retaining conditions; Speed of agitator is 100 rev/mins, cold preservation 36 hours, sucking filtration; 45 ℃ of dryings get Benexate Hydrochloride, and other deposits;
Above-mentioned supercritical CO
28 parts on medicinal residues and Flos Carthami behind the leaching process, 30 parts of Herba Leonuris, 8 parts of Rhizoma Corydalis, 8 parts of the Radixs Linderae, 15 parts of Radix Rehmanniae, 15 parts of Rhizoma Polygoni Cuspidati,
Hold 15 parts and the merging of 15 part of 8 flavor of Semen Plantaginis medical material, decocte with water twice adds 10 times of amounts that water is medical material weight for the first time, decocts 1.5h; Add for the second time 8 times of amounts that water is medical material weight, decoct 1.5h, collecting decoction, relative density is 1.15 when being evaporated to 65 ℃ under 0.07MPa, 60-70 ℃; Add 95% (v/v) ethanol and make the alcohol amount of containing reach 75% (v/v), stir, left standstill 24 hours, decompression filtrate recycling ethanol also concentrates; Relative density was 1.25 when filtrating became to 65 ℃ at concentrating under reduced pressure under 0.07MPa, the 65 ℃ of conditions, and vacuum drying adds above-mentioned Benexate Hydrochloride, pulverized; Add again suitable dextrin, soluble starch, lactose, sucrose, mannitol etc. wherein one or more, with the moistening of an amount of 80% (v/v) ethanol, the system soft material is crossed 14 mesh sieves and is granulated; 50-80 ℃ of drying, 60 order granulate get granule.
Embodiment 3: the preparation of tablet.
Press the preparation of table 1 proportioning raw materials and Tabules conventional method, be prepared into tablet, concrete grammar is following:
Get 20 parts of Rhizoma Curcumae, 15 part of two flavor of Radix Angelicae Sinensis medical material, adopt supercritical CO
2Extract 3h, 60 ℃ of extraction temperature, pressure 40MPa, entrainer are 95% ethanol; Get supercritical extract, adopt saturated water solution method with beta-cyclodextrin inclusion compound, continuous stirring is 5 hours under 70 ℃ of heat-retaining conditions; Speed of agitator is 120 rev/mins, cold preservation 48 hours, sucking filtration; 50 ℃ of dryings, Benexate Hydrochloride, other deposits;
Above-mentioned supercritical CO
210 parts on medicinal residues and Flos Carthami behind the leaching process, 20 parts of Herba Leonuris, 10 parts of Rhizoma Corydalis, 6 parts of the Radixs Linderae, 20 parts of Radix Rehmanniae, 10 parts of Rhizoma Polygoni Cuspidati,
Hold 20 parts and the merging of 10 part of 8 flavor of Semen Plantaginis medical material, decocte with water twice adds 12 times of amounts that water is medical material weight for the first time, decocts 2h, adds 10 times of amounts that water is medical material weight the second time; Decoct 2h, collecting decoction, relative density is 1.20 when being evaporated to 65 ℃ under 0.07MPa, 60-70 ℃, adds 95% (v/v) ethanol and makes and contain the alcohol amount and reach 85% (v/v); Stir, left standstill 24 hours, decompression filtrate recycling ethanol also concentrates, and filtrating relative density when concentrating under reduced pressure becomes to 65 ℃ under 0.07MPa, the 65 ℃ of conditions is 1.30; Vacuum drying adds above-mentioned Benexate Hydrochloride, pulverizes, and adds one or more adjuvants wherein such as starch, dextrin, Icing Sugar, lactose, microcrystalline Cellulose again; Mix homogeneously, with the moistening of an amount of 80% (v/v) ethanol, the system soft material is crossed 30 mesh sieves and is granulated; In 70～80 ℃ of dryings, with 60 mesh sieve granulate, tabletting, sugar coating; Packing, outer package, it is qualified to inspect by ready samples, gets tablet.
Embodiment 4: the preparation of oral liquid.
Press the preparation of table 1 proportioning raw materials and oral liquid formulation conventional method, be prepared into oral liquid, concrete grammar is following:
Get 10 parts of Rhizoma Curcumae, 25 part of two flavor of Radix Angelicae Sinensis medical material, adopt supercritical CO
2Extract 1h, 20 ℃ of extraction temperature, pressure 20MPa, entrainer are 95% ethanol; Get supercritical extract, adopt saturated water solution method with beta-cyclodextrin inclusion compound, continuous stirring is 3 hours under 30 ℃ of heat-retaining conditions; Speed of agitator is 80 rev/mins, cold preservation 24 hours, sucking filtration; 40 ℃ of dryings, Benexate Hydrochloride, other deposits;
Above-mentioned supercritical CO
26 parts on medicinal residues and Flos Carthami behind the leaching process, 40 parts of Herba Leonuris, 6 parts of Rhizoma Corydalis, 10 parts of the Radixs Linderae, 10 parts of Radix Rehmanniae, 20 parts of Rhizoma Polygoni Cuspidati,
Hold 10 parts and the merging of 20 part of 8 flavor of Semen Plantaginis medical material, decocte with water twice adds 8 times of amounts that water is medical material weight for the first time, decocts 1h; Add for the second time 6 times of amounts that water is medical material weight, decoct 1h, collecting decoction, relative density is 1.10 when being evaporated to 65 ℃ under 0.07MPa, 60-70 ℃; Add 95% (v/v) ethanol and make the alcohol amount of containing reach 65% (v/v), stir, left standstill 24 hours, decompression filtrate recycling ethanol also concentrates; Relative density was 1.20 when filtrating became to 65 ℃ at concentrating under reduced pressure under 0.07MPa, the 60 ℃ of conditions, added above-mentioned Benexate Hydrochloride, filtered; Packing, oral liquid is processed in sterilization.
Embodiment 5-12 is all according to table 1 proportioning raw materials, and conventional formulation method in the employing pharmaceutics, adds corresponding adjuvant dosage form preparation.
Embodiment 13: to pharmacodynamic action research in the body of pelvic inflammatory disease
1 materials and methods
1.1 experiment medicine granule of the present invention is by embodiment 2 method for preparinies preparations (Nanjing Women and Children Healthcare Hospital provides, lot number 20101203); (Zhuzhou Qianjin Pharmacy Co., Ltd produces FUKE QIANJIN PIAN, lot number: 201002064).
1.2 instrument and reagent
1.2.1 instrument DKZ-2 type electric heating constant temperature water bath (going up the grand experimental facilities company limited of Nereid), 8452A type ultraviolet spectrophotometer (hewlette-packard); TG328A type photoelectric analytical balance (Shanghai balance equipment factory).
1.2.2 reagent glacial acetic acid (Shanghai reagent four factories, lot number: 090402) Yi Wensilan (Chemical Reagent Co., Ltd., Sinopharm Group, lot number: 2010053)
1.3 210 of animal Kunming mouses are female, body weight (20.1 ± 2.4) g provides (SYXK (Soviet Union) 2000-0053) by Nanjing University of Traditional Chinese Medicine's Experimental Animal Center.
1.4 analgesic activity (hot plate method) experiment
1.4.1 divide into groups and administration divide 5 groups at random with 50 rats, i.e. negative control group, positive controls, the large, medium and small dose groups of granule of the present invention, every group is 10; Next day gastric infusion, continuous 7d, the large, medium and small dosage of granule of the present invention is respectively 40,20,10gkg-1d-1 (quite crude drug); Positive controls is given FUKE QIANJIN PIAN, and dosage is 20gkg-1d-1 (quite crude drug); Negative control group is irritated stomach 0.9% sodium chloride solution, and volume is every 2ml, carries out analgesic activity (hot plate method) experiment behind the drug withdrawal 24h.
1.4.2 with the water bath topped up with water; Make water surface contact hot plate, regulate thermostat and make water temperature be controlled at 55 ± 0.5 ℃, the heating back is as thermostimulation; (survey altogether 2 times as the pain threshold of this Mus to occurring licking the sufficient time (s) from dropping into hot plate with stopwatch record mice; Be no more than 60 seconds persons for qualified with meansigma methods), in administration 1h after the 3rd day, survey every pain threshold again.
1.5 analgesic activity (writhing method) experiment
1.5.1 divide into groups and administration divide 5 groups at random with 50 rats, i.e. negative control group, positive controls, the large, medium and small dose groups of granule of the present invention, every group is 10; Next day gastric infusion, continuous 7d, the large, medium and small dosage of granule of the present invention is respectively 40,20,10gkg-1d-1 (quite crude drug); Positive controls is given FUKE QIANJIN PIAN, and dosage is 20.21gkg-1d-1 (quite crude drug); Negative control group is irritated stomach 0.9% sodium chloride solution, and volume is every 2ml, carries out analgesic activity (writhing method) experiment behind the drug withdrawal 24h.
1.5.2 after the administration in the 3rd day 1 hour, every Mus lumbar injection 0.6% glacial acetic acid 0.1ml/10g, behind the record injection algogen in the 10min each Mus turn round the body number of times, the comparable group differences.
1.6 granulation tissue hyperplasia test
1.6.1 divide into groups and experiment divide 5 groups at random with 50 rats, i.e. model group, positive controls, the large, medium and small dose groups of granule of the present invention, every group is 10, adopts cotton balls oxter burying storage modeling.
1.6.2 administration gastric infusion next day, continuous 7d, the large, medium and small dosage of granule of the present invention is respectively 40,20,10gkg-1d-1 (quite crude drug); Positive controls is given FUKE QIANJIN PIAN, and dosage is 20gkg-1d-1 (quite crude drug); Model group is irritated stomach 0.9% sodium chloride solution, and volume is every 2ml, and 30min after the last administration with sacrifice of animal, takes out the cotton balls granulation tissue, in 60 ℃ of baking boxs, dries and weighs.Deduct the cotton balls quality with cotton balls granulation tissue dry weight, be the granulation tissue net weight.
1.7 the influence of mouse peritoneal capillary permeability
1.7.1 divide into groups and administration divide 6 groups at random with 60 rats, i.e. negative control group, model group, positive controls, the large, medium and small dose groups of granule of the present invention, every group is 10; Next day gastric infusion, continuous 7d, the large, medium and small dosage of granule of the present invention is respectively 40,20,10gkg-1d-1 (quite crude drug); Positive controls is given FUKE QIANJIN PIAN, and dosage is 20gkg-1d-1 (quite crude drug); Negative control group and model group are irritated stomach 0.9% sodium chloride solution, and volume is every 2ml, carry out the influence experiment of abdominal cavity capillary permeability behind the drug withdrawal 24h.
1.7.2 after last 1 administration 1 hour; Each Mus is tail vein injection 0.5% Yi Wensilan normal saline solution 0.1ml/10g body weight successively, and lumbar injection 0.6% glacial acetic acid 0.2ml/ only takes off cervical vertebra and puts to death after 20 minutes immediately; Inject the 5ml normal saline toward intraperitoneal; Gently rub mouse web portion, so that the intraperitoneal dyestuff fully is dissolved in normal saline, and mix homogeneously.Cut off the abdominal cavity integumentary musculature, inhale with No. 6 syringe needles and remove cleaning mixture, add normal saline after the merging to 10ml, centrifugal 10 minutes of 3000rpm gets supernatant 590nm place's colorimetric determination, on standard curve, finds mouse peritoneal again and infiltrates amount of dye, organizes a t check.
1.8 the statistical method data are represented with x ± s, the t check.
2 results
Turn round the body number of times 2.1 the big-and-middle dosed administration group of granule of the present invention can improve the mice pain threshold with minimizing acetic acid induced mice, compare, significant difference (P＜0.05 or P＜0.01) is arranged, show that granule of the present invention has significant analgesia role with negative control group.See table 2.Size dosed administration group can alleviate granulation tissue weight; The cotton balls oxter buried bring out granulation tissue hyperplasia the obvious suppression effect is arranged; With negative control group significant difference (P＜0.01, P＜0.05) is arranged relatively, show that granule of the present invention has tangible antiinflammatory action.See table 3.
2.2 in mouse peritoneal capillary permeability influence test; Model group mouse peritoneal inner dye amount is about four times of negative control group; And the big-and-middle dose groups animal of granule of the present invention abdominal cavity dyestuff all is less than model group, compares with model group, and significant difference (P＜0.01 is arranged; P＜0.05), shows that granule of the present invention can suppress glacial acetic acid and cause increasing of mouse peritoneal capillary permeability.See table 4.
Table 2 granule of the present invention is to the influence of mice analgesic activity (x ± s)
Compare with negative control group,
*1P＜0.01,
*2P＜0.05
Table 3 granule of the present invention buries the influence of bringing out granulation tissue hyperplasia (x ± s) to mice oxter cotton balls
Compare with negative control group,
*1P＜0.01,
*2P＜0.05
Table 4 granule of the present invention increases influence (x ± s) to glacial acetic acid induced mice abdominal cavity capillary permeability
Annotate: compare with model group,
*1P＜0.01,
*2P＜0.05
3 experiment conclusion
3.1 granule of the present invention has significant analgesia role and tangible antiinflammatory action, and the effect of expanding the microcirculatory vascular caliber is preferably arranged, thereby plays the effect of microcirculation improvement.
3.2 suppressing glacial acetic acid, granule of the present invention causes increasing of mouse peritoneal capillary permeability.
3.3 granule of the present invention has curative effect preferably to pelvic inflammatory disease.
Embodiment 14: to the external pharmacodynamic action research (granule extracorporeal bacteria inhibitor test of the present invention) of pelvic inflammatory disease
1. test material
1.1 medicine and reagent
Granule of the present invention is by embodiment 2 method for preparinies preparations (Nanjing Women and Children Healthcare Hospital provides, lot number 20101203)
(Zhuzhou Qianjin Pharmacy Co., Ltd produces FUKE QIANJIN PIAN, lot number: 201002064).
Penicillin sodium: Huabei Pharmaceutic Co., Ltd's specification: 800,000 units/bottle lot number: Y0905124
Sodium chloride, Shanghai hundred million chemical reagent company limiteies of a specified duration, lot number: 00060120.
Nutrient broth, CAS Shanghai Insect Bio Technological Development Co., Ltd., lot number: 090223.
Nutrient agar, microorganism reagent factory of Jiangsu Prov. Disease Preventing and Controlling Center, lot number: 090026.
Calf serum, Hangzhou Sijiqing Biological Engineering Material Co., Ltd., lot number: 090508;
Take off fine sheep blood, available from sheep sheep blood supply station, Nanjing, lot number: 090902
1.2 bacterial strain
The clinical isolates strain: staphylococcus aureus, escherichia coli, B-mode micrococcus scarlatinae, Candida albicans are provided by Jiangsu Prov. People's Hospital microbial room isolation identification.
1.3 instrument and equipment
Electronic balance, Beijing Sai Duolisi balance company limited is produced;
The full-automatic high-pressure autoclave, Japanese SANYO;
Superclean bench, Suzhou Decontamination Equipment Plant;
The water isolation type constant incubator, Shanghai one permanent Science and Technology Ltd.
2 experimental techniques
2.1 increasing bacterium cultivates
Get clinical isolates strain staphylococcus aureus, escherichia coli, B-mode micrococcus scarlatinae, albicans strain a little, be inoculated in respectively in the broth bouillon, cultivate 18h for 37 ℃.(adding 20% calf serum in the B-mode micrococcus scarlatinae broth bouillon) got each bacterial strain nutrient broth culture that 18h cultivates, and does the 10-3 dilution with nutrient broth and is used for experiment.
2.2 doubling dilution (test tube method)
Get 11 in sterilization test tube, the 1st pipe adds nutrient broth fluid medium 1.6mL, all the other every pipe 1mL; Get granule of the present invention respectively and add in first pipe, get 1mL to the 2 pipes behind the mixing, dilution successively; The 10th pipe sucking-off 1mL discards, and the 11st pipe does not add medicinal liquid as contrast.Every pipe adds dilution bacterium liquid 0.1mL, cultivates 24h for 37 ℃, takes out the bacterial growth situation of observing.Muddy like drug liquid tube, promptly represent bacterial growth, supply the reagent thing not have bacteriostasis; Represent then that as drug liquid tube is limpid bacterial growth is suppressed, the medicinal liquid of the greatest dilution that it can bacteria growing inhibiting is the minimal inhibitory concentration (MIC) of this medicine.It is dull and stereotyped that each pipe of limpid medicinal liquid meat soup is changeed kind of bouillon agar, cultivates 24h and observe, and the minimum drug level that does not still have bacterial growth is the bacteriocidal concentration of this medicine.
Get FUKE QIANJIN PIAN (being made into 0.6g/ml), penicillin sodium and (be made into that 160,000 units/mL) as stated above, dilution is successively cultivated, and makes positive control.
3 results
Experimental result shows: granule of the present invention all has stronger bacteriostasis to clinical isolates staphylococcus aureus, escherichia coli, B-mode micrococcus scarlatinae, Candida albicans; MIC shows that 5mg～100mg/mL (pressing the crude drug amount calculates) this preparation has vitro antibacterial activity.See table 5.
Table 5 granule antibacterial activity in vitro of the present invention (n=2)
4 experiment conclusion
4.1 granule of the present invention all has stronger bacteriostasis to clinical isolates staphylococcus aureus, escherichia coli, B-mode micrococcus scarlatinae, Candida albicans; MIC shows that 5mg～100mg/mL (pressing the crude drug amount calculates) granule of the present invention has vitro antibacterial activity.
4.2 granule of the present invention has curative effect preferably to pelvic inflammatory disease.
Claims (10)
1. a Chinese medicine composition that is used to treat pelvic inflammatory disease is characterized in that, it is obtained by the feedstock production of following parts by weight: Rhizoma Curcumae 10-20 part; Radix Angelicae Sinensis 15-25 part, Flos Carthami 6-10 part, Herba Leonuri 20-40 part; Rhizoma Corydalis 6-10 part, Radix Linderae 6-10 part, Radix Rehmanniae 10-20 part; Rhizoma Polygoni Cuspidati 10-20 Fen ， Herba Polygoni Avicularis 10-20 part, Semen Plantaginis 10-20 part.
2. the Chinese medicine composition that is used to treat pelvic inflammatory disease according to claim 1 is characterized in that, it is obtained by the feedstock production of following parts by weight: 15 parts of Rhizoma Curcumae; 20 parts of Radix Angelicae Sinensis, 8 parts on Flos Carthami, 30 parts of Herba Leonuris; 8 parts of Rhizoma Corydalis, 8 parts of the Radixs Linderae, 15 parts of Radix Rehmanniae; 15 parts of Rhizoma Polygoni Cuspidati 15 Fen ， Herba Polygoni Avicularis, 15 parts of Semen Plantaginiss.
3. the Chinese medicine composition that is used to treat pelvic inflammatory disease according to claim 1 and 2 is characterized in that, described Radix Rehmanniae is a Radix Rehmanniae.
4. the Chinese medicine composition that is used to treat pelvic inflammatory disease according to claim 1 and 2 is characterized in that, said Chinese medicine composition adds adjuvant, processes capsule, tablet, granule, pill, drop pill, oral liquid or suppository with conventional formulation method.
5. the described method for preparing that is used to treat the Chinese medicine composition of pelvic inflammatory disease of claim 1 is characterized in that it comprises the steps:
(1) Rhizoma Curcumae, Radix Angelicae Sinensis two flavor medical materials are through supercritical CO
2After the extraction, obtain extract and medicinal residues, medicinal residues are subsequent use, and the extract by adopting saturated water solution method with beta-cyclodextrin inclusion compound, cold preservation, sucking filtration, drying, gets Benexate Hydrochloride;
(2) supercritical CO in the step (1)
2Medicinal residues that obtain after the extraction and Flos Carthami, Herba Leonuri, Rhizoma Corydalis, the Radix Linderae, Radix Rehmanniae, Rhizoma Polygoni Cuspidati 、 Herba Polygoni Avicularis and Semen Plantaginis 8 flavor medical materials merge decocte with water twice, collecting decoction; Relative density is 1.10-1.20 when being concentrated into 65 ℃, adds ethanol and makes the percent by volume that contains the alcohol amount reach 65-85%, stirs; Leave standstill, filter, relative density was 1.20-1.30 and reclaims ethanol when filtrate decompression was concentrated into 65 ℃; The concentrated solution vacuum drying; Add the Benexate Hydrochloride that step (1) obtains, pulverize, mix homogeneously promptly gets.
6. the method for preparing that is used to treat the Chinese medicine composition of pelvic inflammatory disease according to claim 5 is characterized in that, in the step (1), and supercritical CO
2Extraction conditions is: extraction temperature 20-60 ℃, and pressure 20-40MPa, extraction time 1-3h, entrainer are 95% ethanol.
7. the method for preparing that is used to treat the Chinese medicine composition of pelvic inflammatory disease according to claim 5 is characterized in that in the step (1), the beta-cyclodextrin inclusion compound condition is: 30-70 ℃ following continuous stirring 3-5 hour, speed of agitator is 80-120 rev/min; Cold preservation time is 24-48 hour; Baking temperature is 40-50 ℃.
8. the method for preparing that is used to treat the Chinese medicine composition of pelvic inflammatory disease according to claim 5 is characterized in that, in the step (2); Decocting condition is: the 8-12 that adds for the first time water and be medical material weight doubly measures; Decoct 1-2h, the 6-10 that adds water for the second time and be medical material weight doubly measures, and decocts 1-2h.
9. the method for preparing that is used to treat the Chinese medicine composition of pelvic inflammatory disease according to claim 5 is characterized in that, after step (2) finishes; Add adjuvant and process capsule, tablet, granule, pill, drop pill, oral liquid or suppository, or, directly add the Benexate Hydrochloride that step (1) obtains in the concentrated solution after ethanol reclaims in the step (2); Mix homogeneously filters packing; Oral liquid is processed in sterilization.
10. the described Chinese medicine composition that is used for treating pelvic inflammatory disease of claim 1 is in the application for preparing treatment pelvic inflammatory disease medicine.
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| 徐云霞等.慢性盆腔炎中医药治疗研究近况.《中医药临床杂志》.2009,第21卷(第01期), * |
| 韩丽等.盆腔炎的中医治疗进展.《现代中西医结合杂志》.2005,第13卷(第17期), * |
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