CN102188591A - Traditional Chinese medicine for treating colds and preparation method thereof - Google Patents
Traditional Chinese medicine for treating colds and preparation method thereof Download PDFInfo
- Publication number
- CN102188591A CN102188591A CN2011101176341A CN201110117634A CN102188591A CN 102188591 A CN102188591 A CN 102188591A CN 2011101176341 A CN2011101176341 A CN 2011101176341A CN 201110117634 A CN201110117634 A CN 201110117634A CN 102188591 A CN102188591 A CN 102188591A
- Authority
- CN
- China
- Prior art keywords
- parts
- medicine
- radix
- chinese medicine
- relative density
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention relates to a traditional Chinese medicine for treating colds. The medicine is prepared from the following materials: 420 parts of Radix Astragali, 210 parts of ephedra stem, 210 parts of Chinese yam, 420 parts of Seseli mairei, 210 parts of balloonflower root, 210 parts of almond, 210 parts of Sinapis alba, 210 parts of tingli seed, 420 parts of hawthorn, 420 parts of root of large-flowered skullcap, 420 parts of Malan and 210 parts of licorice. The preparation method of the medicine comprises the steps of extraction, alcohol precipitation, condensation and the like. The traditional Chinese medicine of the invention has the efficiency of ventilating lungs and inducing diaphoresis, clearing heat and eliminating phlegm. The medicine is suitable for cough with abundance of phlegm, yellow and sticky phlegm as well as uncomfortable expectoration caused by exogenous cough and phlegm-heat accumulation in lungs. The medicine has definite therapeutic effects and the preparation technology for the medicine is feasible, thus satisfying requirements of industrialized production and clinical use.
Description
Technical field
The invention belongs to a kind of Chinese medicine, particularly, is a kind of Chinese medicine for the treatment of flu, and the present invention also comprises this preparation method of Chinese medicine.
Background technology
The patent No. is 93105425.7, the applying date is on May 5th, 1993, Granted publication day is that the Chinese invention patent on March 29th, 2000 discloses " electuary and the preparation method of treatment infantile common cold ", and this electuary is made by following raw material: Radix Astragali 400-600g, Herba Ephedrae 205-305g, Rhizoma Dioscoreae 205-305g, Radix Seselis mairei 400-600g, Radix Platycodonis 200-350g, Semen Armeniacae Amarum 200-350g, Semen Sinapis Albae 200-350g, Semen Lepidii (Semen Descurainiae) 200-350g, Fructus Crataegi 400-600g, Radix Scutellariae 400-600g, Herba Kalimeridis 400-500g, Radix Glycyrrhizae 200-350g.
This patent also provides the preparation method of this electuary, the steps include: 1, Herba Ephedrae pulverizes, and adds the alcohol reflux secondary, merge extractive liquid; 2, all the other ten pulverizing simply decoct with water secondary, and decoction liquor is filtered, merging filtrate, being evaporated to relative density is 1.00-1.10, is chilled to room temperature, adds equivalent ethanol and stirs, leave standstill, get supernatant and reclaim ethanol, continue to be concentrated into the extractum that relative density is 1.10-1.30; 3, get step 2 gained extractum, in 1: 1.5-3: the ratio of 1.5-3 adds sucrose, dextrin, with step 1 gained Herba Ephedrae alcohol extract mixing, drying, molding, packing.
This patent also discloses a kind of specific embodiment: get Herba Ephedrae 450g and pulverize, add 70% alcohol reflux secondary, merge extractive liquid, is standby; Get Radix Astragali 470g, Rhizoma Dioscoreae 215g, Radix Seselis mairei 420g, Radix Platycodonis 210g, Semen Armeniacae Amarum 220g, Semen Sinapis Albae 230g, Semen Lepidii (Semen Descurainiae) 225g, Fructus Crataegi 500g, Radix Scutellariae 480g, Herba Kalimeridis 410g, Radix Glycyrrhizae 220g, pulverize, decoct with water secondary, each 1.5 hours, decoction liquor is filtered, merging filtrate, being concentrated into relative density is 1.08 (90-95 ℃), is chilled to room temperature, adds equivalent ethanol and stirs, left standstill 12 hours, make its precipitation, get supernatant and reclaim ethanol, continue to be concentrated into the extractum that relative density is 1.20 (60-65 ℃); Get the extractum portion, with two parts of sucrose, with above-mentioned Herba Ephedrae alcohol extract mixing, make granule, drying is made 1000g promptly, packs every bag heavy 8g.
Through research; 93105425.7 the prescription that provided of patent working example be not best prescription; its epheday intermedia 450g has also exceeded the scope of claim protection, prepares medicine and infeasible according to this embodiment, and other specific embodiment is not provided in the patent document yet.Therefore, we optimize best prescription numerical value of the present invention by test, and the result shows that the medicine drug effect that adopts this prescription to make is better.
In addition, 93105425.7 the patent system Preparation Method in the concrete parameter that provided be not optimal parameter yet, and the technological operation of step 3 is not strong, can't realize in the actual production, because the amount of liquid medicine after the Chinese medicine extraction is all bigger, generally all need medicinal liquid is concentrated, could further make dosage form, it is infeasible in 93105425.7 the patent technology Herba Ephedrae extracting solution directly being added in producing granulation.Therefore, we study the concrete parameter of the preparation method of medicine of the present invention, obtain better, the more feasible optimised process of method of drug effect.
Therefore, purpose of the present invention is that the prescription of former patent and technology are screened and optimized, and makes curative effect of medication of the present invention more definite, and technology is more feasible, thereby satisfies the needs of industrialized great production and clinical use.
Summary of the invention
It is feasible to the purpose of this invention is to provide a kind of determined curative effect, few side effects, preparation method, is used for the treatment of the new Chinese medicine of flu.
To achieve these goals, characteristics of the present invention are: it is the medicament of being made by following weight ratio proportion raw material:
420 parts of 210 parts of Radix Seselis mairei of 210 portions of Rhizoma Dioscoreaes of 420 portions of Herba Ephedraes of the Radix Astragali
210 parts of 210 parts of Semen Lepidii (Semen Descurainiae)s of 210 parts of Semen Sinapis Albaes of 210 parts of Semen Armeniacae Amarums of Radix Platycodonis
210 parts in 420 portions of Radix Glycyrrhizaes of 420 parts of Herba Kalimeridis of 420 parts of Radix Scutellariaes of Fructus Crataegi.
The present invention treats the Chinese medicine of flu, except the above raw material, also should comprise adjuvant.This adjuvant can be one or more combination of conventional adjuvant in these pharmaceutical preparatioies such as sucrose, starch, dextrin, magnesium stearate, sorbitol, mannitol, microcrystalline Cellulose, micropowder silica gel, carboxymethyl starch sodium, carboxymethyl cellulose, water, specifically selects any or which is planted adjuvant and should determine according to the required dosage form of making.Because selecting suitable adjuvant according to the dosage form needs is the technology that those of ordinary skills can both grasp, therefore will prepare the required adjuvant of medicine of the present invention and bring in the claimed scope of patent.
According to raw material provided by the present invention and above cited various adjuvants, medicine can be made any dosage form that is suitable for clinical use, for example: tablet, powder, granule, capsule, pill, unguentum, mixture, syrup, injection, obedient agent, suppository etc.For example, with above raw material according to suitable PROCESS FOR TREATMENT after, add one or more the combination of suitable amount of sucrose, starch, dextrin, sorbitol, mannitol, just can make the granule or the capsule of suitable clinical use; Add one or more the combination of suitable amount of sucrose, starch, dextrin, sorbitol, mannitol, magnesium stearate, microcrystalline Cellulose, carboxymethyl starch sodium, just can make the tablet of suitable clinical use; Add one or more the combination of an amount of water, sucrose, sorbitol, mannitol, carboxymethyl cellulose, just can make the oral liquid or the syrup that are fit to clinical use.Other dosage form differs at this and one enumerates, and in a word, no matter adopts which kind of adjuvant to prepare which kind of dosage form, as long as used the identical raw material of the present invention, has all dropped in protection scope of the present invention.
The present invention also provides a kind of preparation method of this medicine:
Described Herba Ephedrae is ground into coarse powder, adds 80% alcohol reflux three times, each 1.5 hours, merge extractive liquid,, decompression recycling ethanol also is concentrated into the clear paste that relative density is 1.08 (55～60 ℃); All the other Radix Seselis mairei etc. ten are ground into coarse powder simply, with 90 ℃ of water rettings three times, each 1 hour, impregnation liquid filters, filtrate merges, being evaporated to relative density is 1.05 (55～60 ℃), puts coldly, adds ethanol to the alcohol amount of containing and reaches 60% of gross mass, stir, left standstill 24 hours, and got supernatant, reclaim ethanol and be concentrated into the clear paste that relative density is 1.21 (55～60 ℃), mix with above-mentioned Herba Ephedrae clear paste, being evaporated to relative density is the extractum of 1.31 (55～60 ℃), adds suitable pharmaceutic adjuvant, is prepared into the pharmaceutical dosage form that is suitable for using clinically.
The preferred dosage form of the present invention is granule, oral liquid, syrup, tablet, capsule.
Though the concrete numerical value of optimum prescription of the present invention is in the numerical range of 93105425.7 patents, 93105425.7 patents do not disclose this concrete numerical value, and therefore this concrete numerical value has novelty.Well-knownly be: the prescription of each new Chinese medicine all needs by very long clinical practice, through revising many times, adjusting, and verify and final to form by animal experiment, clinical trial, it be doctor and countless patient by scientific experimentation and the coefficient result of clinical practice, be not that those of ordinary skills are just confirmable by the test of limited number of times.Although the prescription of this application is identical on raw material type with prescription in first to file, but because Chinese medicine is the system of a complexity, the constituent content difference, its effect of being played in prescription is also different with status (as the monarch relation), its chemical constituent of the product that obtains is also inevitable different, the effect that produces is also different certainly, is embodied in the difference of curative effect aspect and the difference of toxic and side effects aspect etc.
In addition, this preferred concrete technological parameter of application institute not only will rely on the long-term production practical experience to obtain, also need by consulting lot of documents, integrated application single factor experiment method, multifactor, multilevel orthogonal experiment, utilize advanced persons' such as high performance liquid chromatography method to detect, the number statistical analysis of science that data is held water, thus optimal parameter obtained, need to utilize pharmacodynamics, toxicology test method and clinical trial that the result is verified in addition.Because study condition and method limit, being doomed the parameter of each researcher research can not be identical, even individual parameters is identical, also parameter that can not be all is identical, and well-known, Chinese medicine is the system of a complexity, the concrete parameter difference during each technological parameter particularly extracts, resulting ingredient just is not the same, and it is also different to tell on thus.
We further provide prescription of the present invention preferably to test the method that studies in great detail of correlation data and concrete technological parameter hereinafter.
One, preparation method process parameter optimizing research:
1, the extraction process parameter study of Herba Ephedrae
Be to optimize Herba Ephedrae extraction process parameter, choose concentration of alcohol, ethanol consumption, extraction time, three levels of extraction time four factors are tested, and are performance assessment criteria to extract the extractum yield, selected factor level and result of the test are as follows:
Herba Ephedrae Study on extraction factor level table
Herba Ephedrae alcohol extraction process research factor level analytical table L
9(3
4)
Variance analysis
| Soruces of variation | Sum of deviation square | Degree of freedom | Mean square deviation | The F value | Significance |
| A | 7.0963 | 2 | 3.5481 | 11.43 | ＞0.05 |
| B | 14.8940 | 2 | 7.4470 | 23.99 | ＜0.05 |
| C | 42.7948 | 2 | 21.3974 | 68.93 | ＜0.05 |
| D | 0.6208 | 2 | 0.3104 |
F
0.05(2，2)＝19.0 F
0.01(2，2)＝99.0
Know that by variance analysis factor C (reflux, extract, number of times) has appreciable impact to extract yield, should select C3 by above-mentioned analysis; Factor B (ethanol consumption) has considerable influence, but consumption is excessive, causes cost too high; Consider that concentration of alcohol low consumption ethanol is few; Extraction time is short, and it is low to consume energy, and comprehensive commercial production situation is chosen A2B1C3D2 technology, promptly adds 10 amounts, 80% alcohol reflux 3 times, and each 1.5 hours process program is more reasonable.As can be seen from the above results, the effect that adopts the preferred technological parameter of test to extract will be significantly better than the effect that adds 70% alcohol reflux secondary of former invention employing.
2, Radix Scutellariae etc. ten medical material extraction process by water parameter study simply
Be to optimize the extraction process by water parameter, choose amount of water, extraction time, extraction time, three horizontal parameters of four factors of temperature and test, the extractum yield is extracted in examination, and factor level and result of the test see the following form:
Ten Herba indigoferae Pseudotinctoriae water extraction process such as Radix Scutellariae research factor level table
Ten Herba indigoferae Pseudotinctoriae water extraction process such as Radix Scutellariae research factor level analytical table L
9(3
4)
Variance analysis
| Soruces of variation | Sum of deviation square | Degree of freedom | Mean square deviation | The F value | Significance |
| A | 11.2860 | 2 | 5.6430 | 2.76 | ＞0.05 |
| B | 4.0862 | 2 | 2.0431 | 1 | |
| C | 108.6538 | 2 | 54.3269 | 26.59 | ＜0.05 |
| D | 41.2680 | 2 | 20.6340 | 10.10 | ＞0.05 |
F
0.05(2，2)＝19.0 F
0.01(2，2)＝99.0
The result shows that factor C (extraction time) carries the extractum yield to water and has the greatest impact, and should select level 3; Gap is little between the other factors level, considers to reduce cost that other factors is chosen as A1B1D2, promptly adds 6 times of water gagings, and 90 ℃ of dippings extract three times, and each 1 hour process program is comparatively reasonable.
3, medical material water extract concentrated extract alcohol precipitation process parameter study:
Be to optimize the alcohol precipitation process parameter, choose the extractum relative density, contain the alcohol amount, three horizontal parameters of three factors of time of repose are tested, the extractum yield is extracted in examination, and factor level and result of the test see the following form:
Ten Herba indigoferae Pseudotinctoriae water extraction extractum alcohol precipitation processes such as Radix Scutellariae research factor level table
Ten Herba indigoferae Pseudotinctoriae water extraction extractum alcohol precipitation processes such as Radix Scutellariae research factor level analytical table L
9(3
4)
Variance analysis
F
0.05(2，2)＝19.0 F
0.01(2，2)＝99.0
The result shows that factor B (ethanol content) has the greatest impact to water extract-alcohol precipitation extractum yield, should select level 3; Secondly be factor A (extractum is to density), yield height when density is low, but volume and ethanol consumption when considering the medicinal liquid processing, selection level 2; Gap is little between factor C (time of repose) level, consider from the angle that shortens man-hour, optional water intaking is flat 1, but considers from the seriality aspect that produces, leave standstill 12 hours not too suitable, because do not meet the daily schedule of general factory, the proper extension time of repose can make medicinal liquid limpider in addition, and content of effective is higher, therefore take all factors into consideration, finally select C3.Reasonably alcohol precipitation process should be A2B3C3, i.e. extractum relative density 1.05 (55～60 ℃) during precipitate with ethanol add ethanol and make and contain the alcohol amount and reach 60%, leave standstill 24 hours.
Two, the preferred test of prescription correlation data
1, sample preparation
Feed intake by following prescription and to prepare 4 samples respectively:
The lower limit of the prescription numerical range that sample 1:93105425.7 patent is asked for protection
The higher limit of the prescription numerical range that sample 2:93105425.7 patent is asked for protection
The disclosed numerical value of sample 3:93105425.7 patent specific embodiment
Sample 4: the prescription values that this patent application is asked for protection.
Concrete prescription feeds intake and sees the following form:
| Sample 1 | Sample 2 | Sample 3 | Sample 4 | |
| The Radix Astragali | ?400 | ?600 | ?470 | ?420 |
| Herba Ephedrae | ?205 | ?305 | ?450 | ?210 |
| Rhizoma Dioscoreae | ?205 | 305 | 215 | 210 |
| Radix Seselis mairei | ?400 | 600 | 420 | 420 |
| Radix Platycodonis | ?200 | 350 | 210 | 210 |
| Semen Armeniacae Amarum | ?200 | 350 | 220 | 210 |
| Semen Sinapis Albae | ?200 | 350 | 230 | 210 |
| Semen Lepidii (Semen Descurainiae) | ?200 | 350 | 225 | 210 |
| Fructus Crataegi | ?400 | 600 | 500 | 420 |
| Radix Scutellariae | ?400 | 600 | 480 | 420 |
| Herba Kalimeridis | ?400 | 500 | 410 | 420 |
| Radix Glycyrrhizae | ?200 | 350 | 220 | 210 |
More than 4 samples all adopt following prepared: described Herba Ephedrae is ground into coarse powder, adds 80% alcohol reflux three times, each 1.5 hours, merge extractive liquid,, decompression recycling ethanol also is concentrated into the clear paste that relative density is 1.08 (55～60 ℃); All the other Radix Seselis mairei etc. ten are ground into coarse powder simply, with 90 ℃ of water rettings three times, each 1 hour, impregnation liquid filters, filtrate merges, being evaporated to relative density is 1.05 (55～60 ℃), puts coldly, adds ethanol and reaches 60% to containing alcohol amount, stir, left standstill 24 hours, and got supernatant, reclaim ethanol and be concentrated into the clear paste that relative density is 1.21 (55～60 ℃), mix with above-mentioned Herba Ephedrae clear paste, being evaporated to relative density is the extractum of 1.31 (55～60 ℃), with the ratio adding Icing Sugar of this extractum in 1: 3, makes granule.
2, antitussive test
Get 48 of mices, body weight 202 ± 47g, the conventional raising after 2-3 days is divided into 4 groups, 12 every group at random.Get above-mentioned 4 kinds of samples, be made into the aqueous solution of 40% concentration respectively with eliminating cold for resuscitation water.Each treated animal continuous oral administration 7 days, 1 time/day, dosage 0.5ml]/10g.The last administration after 60 minutes mice placed multi-functional lure to cough to draw breathe heavily instrument, 25% strong aqua ammonia spraying 30 seconds, record finishes to be incubation period to the time that cough occurs from spraying, surpass 90 seconds by 90 seconds.The cough number of times of mice in the continuous record 2 minutes, cough standard are that mice occurs fast that abdominal muscle shrinks, and magnify mouth, or with cough sound.Result of the test sees the following form:
| Group | Cough latent period (second) | Cough number of times (inferior) |
| Sample 1 | 7.3±0.36 | 84.4±9.31 |
| Sample 2 | 9.8±2.15 | 61.3±10.00 |
| Sample 3 | 8.6±3.57 | 73.1±8.24 |
| Sample 4 | 12.0±3.58 | 58.6±16.38 |
The result shows: the effect that sample 4 prolongs cough latent period, minimizing cough number of times obviously is better than other three samples.
3, the experiment of eliminating the phlegm
Get a certain amount of phenol redly, accurate claim surely,, make every milliliter of solution that contains phenol red 100Pg with the 5%NaHCO dissolving.Dilution successively is made into every milliliter respectively and contains 1,2,3,4,5,6,7,8, the 9 phenol red solution of μ then.Ultraviolet spectrophotometer, the OD value is detected at wavelength=546nm place, is abscissa with phenol red content, and the OD value is a vertical coordinate, the drawing standard curve.
Animal is conventional to be raised after 2～3 days, was divided into five groups at random.Each organizes mice continuous oral administration 7 days, 1 time/day, dosage 0.5ml/10g.After the last administration 30 minutes, the subcutaneous lumbar injection 0.6% phenol red normal saline 0.5ml/ in mouse carotid back only injects after 30 minutes, take off cervical vertebra and put to death mice, carefully open the thoracic cavity, the complete bilateral lungs of peeling off place the test tube that fills the 2ml normal saline, vibrated 20～30 minutes, add 1mol/L sodium hydroxide 0.2ml, mixing makes the solution alkalize, ultraviolet spectrophotometer calculates phenol red excretion amount in λ=546nm place photometry density according to phenol red standard curve.
Sample sees the following form to the influence of the phenol red excretion of mice trachea:
| Group | ?n | Respiratory tract phenol red concentration C/ μ g.ml -1 |
| Blank | ?12 | 2.56±1.03 |
| Sample 1 | ?12 | 3.17±1.29 |
| Sample 2 | ?12 | 5.54±1.70* |
| Sample 3 | ?12 | 4.91±1.58* |
| Sample 4 | ?12 | 6.62±1.96* |
Annotate: compare * P＜0.01 with the blank group
Sample 1,2,3,4 all can improve the phenol red excretion amount in mouse breathing road to some extent, but sample 1 (lower limit of the scope of promptly writing out a prescription) compares there was no significant difference with the blank group, sample 2,3,4 and blank group relatively have significant difference (P＜0.01), and the effect that sample 4 improves the phenol red excretion amount in mouse breathing road obviously is better than other 3 samples.
More than test shows: its drug effect of sample that adopts the patent prescription of this application to make obviously is better than 93105425.7 patent prescription.
Three, technology correlation data
1, sample preparation
Adopt the patented method of 93105425.7 patented methods and this application to prepare two samples respectively, carry out drug effect relatively.
The prescription that feeds intake is:
420 parts of 210 parts of Radix Seselis mairei of 210 portions of Rhizoma Dioscoreaes of 420 portions of Herba Ephedraes of the Radix Astragali
210 parts of 210 parts of Semen Lepidii (Semen Descurainiae)s of 210 parts of Semen Sinapis Albaes of 210 parts of Semen Armeniacae Amarums of Radix Platycodonis
210 parts in 420 portions of Radix Glycyrrhizaes of 420 parts of Herba Kalimeridis of 420 parts of Radix Scutellariaes of Fructus Crataegi.
Sample 1 preparation method: get Herba Ephedrae and pulverize, add 70% alcohol reflux secondary, merge extractive liquid, is standby; All the other Radixs Astragali etc. ten are pulverized simply, decoct with water secondary, each 1.5 hours, decoction liquor was filtered, merging filtrate, being concentrated into relative density is 1.08 (90-95 ℃), is chilled to room temperature, adds equivalent ethanol and stirs, left standstill 12 hours, make its precipitation, get supernatant and reclaim ethanol, continue to be concentrated into the extractum that relative density is 1.20 (60-65 ℃); Get the extractum portion, with three parts of sucrose, with above-mentioned Herba Ephedrae alcohol extract mixing, make granule.
Sample 2 preparation methoies: get Herba Ephedrae and be ground into coarse powder, add 80% alcohol reflux three times, each 1.5 hours, merge extractive liquid,, decompression recycling ethanol also is concentrated into the clear paste that relative density is 1.08 (55～60 ℃); All the other Radixs Astragali etc. ten are ground into coarse powder simply, with 90 ℃ of water rettings three times, each 1 hour, impregnation liquid filters, filtrate merges, and being evaporated to relative density is 1.05 (55～60 ℃), puts cold, add ethanol and reach 60% to containing the alcohol amount, stir, left standstill 24 hours, get supernatant, reclaim ethanol and be concentrated into the clear paste that relative density is 1.21 (55～60 ℃), mix with above-mentioned Herba Ephedrae clear paste, being evaporated to relative density is the extractum of 1.31 (55～60 ℃), gets the extractum portion, with three parts of sucrose, make granule.
2, antitussive test
It is identical that concrete test method and above-mentioned prescription are preferably tested control methods.Result of the test sees the following form:
| Group | Cough latent period (second) | Cough number of times (inferior) |
| Sample 1 | 8.7±0.79 | 78.4±6.45 |
| Sample 2 | 12.0±3.58 | 58.6±16.38 |
The result shows: the effect that sample 2 prolongs cough latent period, minimizing cough number of times obviously is better than sample 1.
3, the experiment of eliminating the phlegm
It is identical that concrete test method and above-mentioned prescription are preferably tested control methods.Result of the test sees the following form:
| Group | ?n | Respiratory tract phenol red concentration C/ μ g.ml -1 |
| Sample 1 | ?12 | 4.67±1.58 |
| Sample 2 | ?12 | 6.62±1.96 |
The result shows: the effect that sample 2 improves the phenol red excretion amount in mouse breathing road obviously is better than sample 1.
More than two tests show: adopt the patent prescription of this application and its curative effect of medicine that method makes obviously to be better than 93105425.7 patent and write out a prescription and method.
Other results of pharmacodynamic test:
1, refrigeration function
Triple vaccine causes that the rabbit of heating gavages the visible significantly refrigeration function of medicine of the present invention.Refrigeration function and the comparison of blank group in 3～5 hours after granule 8g/Kg group and the administration of 12g/Kg group, the equal significance of difference (P＜0.05), the effect of 12g/Kg is more obvious, though it is slower than compound aminophenazone that the refrigeration function of medicine of the present invention occurs, but relatively more lasting, embody the Chinese medicine action temperature and characteristics.Result of the test sees the following form:
The refrigeration function of granule:
Annotate: ☆ P＜0.05 ☆ ☆ P＜0.01
2, to Immune Effects
Medicine of the present invention compares there was no significant difference to the phagocytosis and the matched group of mouse macrophage, but the influence of lymhocyte transformation rate is had significant difference, can obviously strengthen LT effect, and prompting this product has certain immunological enhancement.
Medicine of the present invention is to the influence of mouse lymphocyte conversion ratio
3, antibacterial tests
Use dull and stereotyped diffusion method, golden Portugal bacterium, dysentery bacterium, escherichia coli, Bacillus proteus, beta hemolytic streptococcus, bacillus subtilis, green moon bright bacillus, pneumobacillus, Bacillus typhi, candida albicans are done bacteriostatic test.
The result shows: granule concentration is enclosed more than 17mm 33% and 16% pair of golden Portugal bacterium, Bacillus proteus, and bacillus subtilis is between 11～14mm, and dysentery bacterium is more than 13mm, and 8% concentration is 15% to the inhibition zone of golden Portugal bacterium, does not see antibacterial to other strain.
The antimicrobial spectrum report:
The specific embodiment
The present invention is described in detail below in conjunction with embodiment, but should not be construed as limitation of the present invention.
Embodiment 1:
Get Herba Ephedrae 210g and be ground into coarse powder, add 80% alcohol reflux three times, each 1.5 hours, merge extractive liquid,, decompression recycling ethanol also is concentrated into the clear paste that relative density is 1.08 (55～60 ℃); Get Radix Astragali 420g, Rhizoma Dioscoreae 210g, Radix Seselis mairei 420g, Radix Platycodonis 210g, Semen Armeniacae Amarum 210g, Semen Sinapis Albae 210g, Semen Lepidii (Semen Descurainiae) 210g, Fructus Crataegi 420g, Radix Scutellariae 420g, Herba Kalimeridis 420g, Radix Glycyrrhizae 210g, be ground into coarse powder, with 90 ℃ of water rettings three times, each 1 hour, impregnation liquid filters, filtrate merges, being evaporated to relative density is 1.05 (55～60 ℃), puts coldly, adds ethanol and reaches 60% to containing alcohol amount, stir, left standstill 24 hours, and got supernatant, reclaim ethanol and be concentrated into the clear paste that relative density is 1.21 (55～60 ℃), mix with above-mentioned Herba Ephedrae clear paste, being evaporated to relative density is the extractum of 1.31 (55～60 ℃), adds suitable amount of sucrose powder and dextrin, makes granule.
Character: this product is that pale brown color is to brown granule; Sweet, little hardship of distinguishing the flavor of.
Assay: measure according to high performance liquid chromatography (Chinese Pharmacopoeia version appendix in 2010 VI D), this product contains Radix Scutellariae with baicalin (C for every bag
21H
13O
11) meter, must not be less than 16mg.
Function cures mainly: diffusing lung, relieving exterior syndrome, removing heat-phlegm.Be used for the exogenous cough, the cough with copious phlegm due to the phlegm-heat obstructing in the lung, the expectorant Huang is sticky, expectoration is not well.
Specification: every packed 8g.
Usage and dosage: use warm boiled water, one-year-old with interior one time 1/3 bag (being equivalent to raw medicinal herbs 4.76g), one-year-old to three years old one time 2/3 bag (being equivalent to raw medicinal herbs 9.52g), four years old to seven years old one time 1 bag (being equivalent to raw medicinal herbs 14.28g), eight years old to 14 years old one time 1.5 bags (being equivalent to raw medicinal herbs 21.42g), 3 times on the one, 3 days is a course of treatment; Or follow the doctor's advice.
Embodiment 2:
Preparation method is identical with embodiment 1, and different is that the adjuvant that adds comprises water, and the dosage form of making is an oral liquid.
Assay: measure according to high performance liquid chromatography (Chinese Pharmacopoeia version appendix in 2010 VI D), this product contains Radix Scutellariae with baicalin (C for every bottle
21H
13O
11) meter, must not be less than 16mg.
Specification: every bottle of 10ml.
Usage and dosage: oral, one-year-old with interior one time 1/3 bottle (being equivalent to raw medicinal herbs 4.76g), one-year-old to three years old one time 2/3 bottle (being equivalent to raw medicinal herbs 9.52g), four years old to seven years old one time 1 bottle (being equivalent to raw medicinal herbs 14.28g), eight years old to 14 years old one time 1.5 bottles (being equivalent to raw medicinal herbs 21.42g), 3 times on the one, 3 days is a course of treatment; Or follow the doctor's advice.
Embodiment 3:
Preparation method is identical with embodiment 1, and different is that the adjuvant that adds comprises water, sucrose, and the dosage form of making is a syrup.
Embodiment 4:
Preparation method is identical with embodiment 1, and the adjuvant that different is adds is one or more the combination of sucrose, starch, dextrin, sorbitol, mannitol, and the dosage form of making is a capsule.
Embodiment 5:
Preparation method is identical with embodiment 1, and the adjuvant that different is adds is one or more the combination of sucrose, starch, dextrin, sorbitol, mannitol, magnesium stearate, microcrystalline Cellulose, carboxymethyl starch sodium, and the dosage form of making is a tablet.
Claims (3)
1. Chinese medicine for the treatment of flu is characterized in that it is the medicament of being made by following weight ratio proportion raw material:
420 parts of 210 parts of Radix Seselis mairei of 210 portions of Rhizoma Dioscoreaes of 420 portions of Herba Ephedraes of the Radix Astragali
210 parts of 210 parts of Semen Lepidii (Semen Descurainiae)s of 210 parts of Semen Sinapis Albaes of 210 parts of Semen Armeniacae Amarums of Radix Platycodonis
210 parts in 420 portions of Radix Glycyrrhizaes of 420 parts of Herba Kalimeridis of 420 parts of Radix Scutellariaes of Fructus Crataegi.
2. according to the described preparation method of Chinese medicine of claim 1, it is characterized in that:
Described Herba Ephedrae is ground into coarse powder, adds 80% alcohol reflux three times, each 1.5 hours, merge extractive liquid,, decompression recycling ethanol also is concentrated into the clear paste that relative density is 1.08 (55～60 ℃); All the other Radix Seselis mairei etc. ten are ground into coarse powder simply, with 90 ℃ of water rettings three times, each 1 hour, impregnation liquid filters, filtrate merges, being evaporated to relative density is 1.05 (55～60 ℃), puts coldly, adds ethanol to the alcohol amount of containing and reaches 60% of gross mass, stir, left standstill 24 hours, and got supernatant, reclaim ethanol and be concentrated into the clear paste that relative density is 1.21 (55～60 ℃), mix with above-mentioned Herba Ephedrae clear paste, being evaporated to relative density is the extractum of 1.31 (55～60 ℃), adds suitable pharmaceutic adjuvant, is prepared into the pharmaceutical dosage form that is suitable for using clinically.
3. according to the described preparation method of Chinese medicine of claim 2, it is characterized in that this pharmaceutical dosage form is granule, oral liquid, syrup, tablet or capsule.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110117634 CN102188591B (en) | 2011-05-09 | 2011-05-09 | Traditional Chinese medicine for treating colds and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110117634 CN102188591B (en) | 2011-05-09 | 2011-05-09 | Traditional Chinese medicine for treating colds and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102188591A true CN102188591A (en) | 2011-09-21 |
| CN102188591B CN102188591B (en) | 2013-02-13 |
Family
ID=44598026
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110117634 Active CN102188591B (en) | 2011-05-09 | 2011-05-09 | Traditional Chinese medicine for treating colds and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102188591B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102284021A (en) * | 2011-07-25 | 2011-12-21 | 王疆宏 | Lung-releasing and cough-relieving grain composite for children and preparation method and quality control method thereof |
| CN102698048A (en) * | 2012-05-31 | 2012-10-03 | 蒋保珍 | Traditional Chinese medicine composition for treating wind heat bovine influenza |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1094629A (en) * | 1993-05-05 | 1994-11-09 | 王登科 | Children's's QI invigorating detoxifcation electuary and preparation method |
-
2011
- 2011-05-09 CN CN 201110117634 patent/CN102188591B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1094629A (en) * | 1993-05-05 | 1994-11-09 | 王登科 | Children's's QI invigorating detoxifcation electuary and preparation method |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102284021A (en) * | 2011-07-25 | 2011-12-21 | 王疆宏 | Lung-releasing and cough-relieving grain composite for children and preparation method and quality control method thereof |
| CN102284021B (en) * | 2011-07-25 | 2012-07-18 | 王疆宏 | Lung-releasing and cough-relieving grain composition for children and preparation method and quality control method thereof |
| CN102698048A (en) * | 2012-05-31 | 2012-10-03 | 蒋保珍 | Traditional Chinese medicine composition for treating wind heat bovine influenza |
| CN102698048B (en) * | 2012-05-31 | 2013-09-18 | 蒋保珍 | Traditional Chinese medicine composition for treating wind heat bovine influenza |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102188591B (en) | 2013-02-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101322774B (en) | Medicament composition with fatigue-resisting function and preparation method and application thereof | |
| CN101991716A (en) | New application of pharmaceutical composition of vital energy benefiting and smart decoction and the like in prevention and cure of osteoporosis | |
| CN106806853B (en) | Traditional Chinese medicine composition for relieving physical fatigue and/or improving anoxia endurance, preparation method and application thereof | |
| CN101015665A (en) | Medicine composition with sleeping improving function, preparing process and quality controlling means thereof | |
| CN102188591B (en) | Traditional Chinese medicine for treating colds and preparation method thereof | |
| CN101549059B (en) | Medicament for treating bronchial asthma and preparation method thereof | |
| CN103432421A (en) | Medicine composition for improving immunity | |
| CN102784339A (en) | Medicament for treating pulmonary tuberculosis by combined chemotherapy with traditional Chinese medicine Fuying and preparation method | |
| CN1709299A (en) | Chinese medicine formulation for treating asthma and its preparing method | |
| CN102698100A (en) | Oral solution for treating diabetes mellitus | |
| CN115414435A (en) | Chinese herbal compound Ge Houdan poria cocos granules and preparation method and application thereof | |
| CN100448472C (en) | Chinese medicinal formulation for treating pulmonary tuberculosis | |
| CN101491629B (en) | Medicine composition for treating diabetes and preparation method thereof | |
| CN103251827A (en) | Traditional Chinese medicine composition for treating diabetes mellitus and preparation method thereof | |
| CN105727088A (en) | Pharmaceutical composition and preparation method, preparation and application thereof | |
| CN102302691B (en) | Herbal tea for eliminating discomfort of patient after operation and preparation method for herbal tea | |
| CN105396039A (en) | Pharmaceutical composition preventing children from repeated cold and preparation method therefor | |
| CN105168582A (en) | Traditional Chinese medicine composition for children cold and preparation method thereof | |
| CN101810667A (en) | Medicine composition for auxiliary treatment of cancer | |
| CN104491318A (en) | Traditional Chinese preparation used for treating asthma and preparation method of traditional Chinese preparation | |
| CN101342313A (en) | Health care granular formulation, preparation and application thereof | |
| CN104906339B (en) | It is a kind of that there is the health food granule for improving sleep function | |
| CN110613830B (en) | Traditional Chinese medicine preparation for treating chronic obstructive pulmonary disease | |
| CN1327821A (en) | Capsule for treating cough and asthma | |
| CN106692748A (en) | Medicine for preventing and treating heatstroke of pigs and preparation method of medicine |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee |
Owner name: JIANMIN GROUP YEKAITAI MEDICINE (SUIZHOU) CO., LTD Free format text: FORMER NAME: SUIZHOU PHARMACEUTICAL CO., LTD., JIANMIN GROUP, WUHAN CITY |
|
| CP01 | Change in the name or title of a patent holder |
Address after: 441300 Suizhou economic and Technological Development Zone, Hubei, Suizhou Patentee after: Thailand Jianmin group vane (Suizhou) Co. Ltd. medicine Patentee after: JIANMIN PHARMACEUTICAL GROUPS CORP., LTD. Address before: 441300 Suizhou economic and Technological Development Zone, Hubei, Suizhou Patentee before: Suizhou Pharmaceutical Co., Ltd., Jianmin Group, Wuhan City Patentee before: Wuhan Jianmin Pharmaceutical Group Co., Ltd. |