CN102183510A - Colloidal gold detecting method and device based on digital image processing - Google Patents
Colloidal gold detecting method and device based on digital image processing Download PDFInfo
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Abstract
The invention relates to a colloidal gold detecting method based on digital image processing, comprising the following steps of: collecting RGB (Red, Green, Blue) coloured images by an area array CCD (Charge Coupled Device) detector, carrying out gray level conversion, edge extraction, background suppression and the like on the collected coloured images, and finally quantitatively calculating the corresponding concentration result of a sample. The invention also relates to a colloidal gold detecting device based on the digital image processing; and the device can be used for detecting the colloidal gold by the method provided by the invention so that the device not only realizes the negative and positive qualitative judgement of the sample but also realizes the quantitative application on the lower end.
Description
Technical field
The present invention relates to the human body fluid technical field of immunoassay, particularly relate to a kind of collaurum detection method and device thereof based on Digital Image Processing.
Background technology
Contain health whether important information in the human body fluid, comprise the existence of height, specific protein concentration change, pathogen and corresponding antibodies thereof of various hormone-contents and what etc., these project major parts to be measured can be detected with colloid gold immune infiltration technology (gold marking spot method), testing result is that the observing response plate has or not punctation to present, the depth of punctation is directly proportional with the concentration of measured object, but only depend on eyes to carry out qualitative interpretation to it, can't be quantitative.
The gold marking spot method has been widely used in the mensuration of human chorionic gland promoting sexual gland hormone (HCG) in serum or the urine.At present, a kind of gold marking spot standard measure readout instrument (U2 instrument) can carry out the mensuration of HCG, because the photodiode that its detector that adopts of U2 instrument is a single-point, so it can only be mingled in the signal in zone to be detected in the instrument; Its light source that adopts is single led equally in addition, thus its be radiated on the reaction plate light inadequately evenly and because the characteristics of LED itself make also instability of its luminous intensity.Especially when the sample of gold marking spot method low concentration is tested, because the U2 instrument only can carry out analytical calculation to the photocurrent power that photosensitive tube produces, so the U2 instrument is low even can't quantitatively discern in low side resolution.
Summary of the invention
Technical matters to be solved by this invention provides a kind of collaurum detection method and device thereof based on Digital Image Processing, can realize the quantitative identification of gold marking spot method low concentration sample.
The technical solution adopted for the present invention to solve the technical problems is: a kind of collaurum detection method based on Digital Image Processing is provided, may further comprise the steps:
(1) obtains the RGB coloured image of reaction plate;
(2) the RGB coloured image that obtains is converted into gray level image;
(3) utilize edge algorithms from gray level image, to extract the edge of target area to be analyzed;
(4) determine the center of spot in the image, distinguish central area and neighboring area;
(5) in central area and neighboring area, select four test zones respectively, and the pixel in the test zone is compared the concentration of quantitative Analysis sample.
Obtained the RGB coloured image of reaction plate in the described step (1) by the area array CCD detector, described reaction plate is subjected to the irradiation of area source.
Also comprise following substep in the described step (4):
(a) establish a square area in picture centre, the center of described square area is overlapped with the test paper center;
(b) around image, be provided with four rectangular areas;
(c) position of spot is determined in the requirement that must cover described square area according to spot and can not enter any one described rectangular area.
All contain 3600 pixels in each test zone of selecting in the described step (5).
The device of a kind of use based on the collaurum detection method of Digital Image Processing also is provided, comprise light source, detector, DSP image digitization processing platform and display, described DSP image digitization processing platform comprises image conversion module, edge extracting module, speckle displacement determination module, zone selection module and the comparison computing module that links to each other successively; Described light source is an area source, with rayed on reaction plate; Described detector is the area array CCD detector, is used to obtain the RGB coloured image of reaction plate; Described image conversion module is used for the RGB coloured image is converted to gray level image; Described edge extracting module is used for extracting from gray level image the edge of target area to be analyzed; Described speckle displacement determination module is used for determining the center of image spot; Described zone selects module to be used for selecting four test zones respectively in speckle regions and neighboring area; Described relatively computing module is used for the pixel of the test zone of selecting is compared, and the concentration of quantitative Analysis sample; Described display is used to show the concentration results of sample correspondence.
Described speckle displacement determination module comprises regional setting module and position determination module; Described regional setting module is used for establishing a square area and set four rectangular areas around image in picture centre; The position of spot is determined in the requirement that described position determination module must cover described square area according to spot and can not enter any one described rectangular area.
All contain 3600 pixels in each test zone that described zone selects module to select.
Beneficial effect
Owing to adopted above-mentioned technical scheme, the present invention compared with prior art, have following advantage and good effect: adopt the area array CCD detector to replace the photodiode of single-point in the U2 instrument, so can obtain a width of cloth digital image of target area, for Flame Image Process provides good basis.Adopt single led the comparing in area source and the U2 instrument, the homogeneity of its irradiation and the stability of luminous intensity are guaranteed.The image that opposite battle array ccd detector collects carries out after processing such as gradation conversion, edge extracting, the background inhibition operation finally rapidly and accurately that quantitative Analysis goes out the concentration results of sample correspondence, thereby can realize the quantitative identification of gold marking spot method low concentration sample.
Description of drawings
Fig. 1 is a process flow diagram of the present invention;
Fig. 2 is a spot of the present invention location synoptic diagram;
Fig. 3 is center of the present invention spot and neighboring area image screening synoptic diagram;
Fig. 4 is a structured flowchart of the present invention.
Embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.Should be understood that in addition those skilled in the art can make various changes or modifications the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
The present invention relates to a kind of collaurum detection method based on Digital Image Processing, this method is to collecting after digital image carried out gradation conversion, edge extracting, background inhibition etc. and handle operation, final quantitative calculation goes out the concentration results of sample correspondence, utilize this method not only can realize the qualitative judgement of sample yin and yang attribute, can also realize quantitative application at low side.Its concrete steps may further comprise the steps as shown in Figure 1:
(1) closely obtains the RGB coloured image of reaction plate by the area array CCD detector, wherein, reaction plate is subjected to the irradiation of area source, be subjected to light more even owing to adopt area source to replace original single led reaction plate that makes, and the luminous intensity of area source is also more stable.Adopt the area array CCD detector to replace the photodiode of single-point, so can obtain a width of cloth digital image of target area, for Flame Image Process provides good basis.
(2) the RGB coloured image that obtains is converted into gray level image, can uses floating-point arithmetic to realize usually, promptly get Gray=R*0.3+G*0.59+B*0.11, with original RGB (R, G, B) R in, G, B is unified to be replaced with Gray, forms new color RGB (Gray, Gray, Gray), replace original RGB (R, G with it, B), just become gray-scale map.
(3) utilize edge algorithms from gray level image, to extract the edge of target area to be analyzed;
(4) determine the center of spot in the image, distinguish central area and neighboring area.Because this method is come identification plate colour developing situation based on Flame Image Process, so higher to the status requirement of reaction plate spot.As shown in Figure 2, the image range that the area array CCD detector is obtained is rectangle (being the rectangle that has oblique line among Fig. 2), this rectangle size is made as 10.70*8.56mm (720*526 pixel), diameter (being the diameter of test paper) at this assumed response plate center pit is 7.4mm (being white background border circular areas among Fig. 2), and this moment, the diameter range of spot was exactly the center of determining.In this step, also comprise following substep, comprising the pixel gray-scale value in two kinds of zones, i.e. central area pixel and neighboring area pixel:
(a) establish a square area in picture centre, it is the dashed square at Fig. 2 center, the size of this square area is made as 1.93*1.93mm (130*130 pixel), the center of described square area and test paper center heavy and, in described square area, choose the central area pixel;
(b) around image, be provided with four rectangular areas, i.e. dashed rectangle among Fig. 2, the size of each rectangular area is made as 1.78*0.45mm (120*30 pixel), chooses the neighboring area pixel in described rectangular area;
(c) position of spot is determined in the requirement that must cover described square area according to spot and can not enter any one described rectangular area.That is to say that get a little correctly in order to guarantee the central area, spot must cover described square area; In order to guarantee that the neighboring area gets a little correctly, spot can not enter in any one described rectangular area.The position of spot is carried out following definite according to above-mentioned 2 requirements: at first, it is the center of circle that spot should cover with the test paper center, diameter should be greater than the cornerwise border circular areas of square area the value of central area (guarantee cover), obtains diameter according to the big I of the square area of above-mentioned hypothesis to be
Secondly, it is the center of circle that the edge of spot can not surpass with the test paper center, diameter should be less than the border circular areas (guaranteeing not enter the neighboring area value) of distance between short relative rectangular area, the distance of two groups of rectangular areas among Fig. 2 is identical, and, be that 3.6mm (＜test paper diameter/2) can guarantee not enter the neighboring area value so locate diameter according to the size of rectangular area.Therefore, the diameter of spot should be greater than interior diameter of a circle among Fig. 2 less than the diameter of cylindrical.So pass through to determine the position of spot, thereby central area and neighboring area are distinguished.
(5) has the shortcoming of unevenness for fear of the spot of collaurum through in reaction plate, forming after the diafiltration, in central area and neighboring area, select four test zones (be among Fig. 3 four square area on the spot and four rectangular areas on the test paper) respectively, and the pixel in the test zone compared the concentration of quantitative Analysis sample.Wherein, each test zone contains 3600 pixels.
The invention still further relates to the device of a kind of use based on the collaurum detection method of Digital Image Processing, as shown in Figure 4, comprise light source, detector, DSP image digitization processing platform and display, described DSP image digitization processing platform comprises image conversion module, edge extracting module, speckle displacement determination module, zone selection module and the comparison computing module that links to each other successively; Described light source is an area source, with rayed on reaction plate; Described detector is the area array CCD detector, is used to obtain the RGB coloured image of reaction plate; Described image conversion module is used for the RGB coloured image is converted to gray level image; Described edge extracting module is used for extracting from gray level image the edge of target area to be analyzed; Described speckle displacement determination module is used for determining the center of image spot; Described zone selects module to be used for selecting four test zones respectively in speckle regions and neighboring area, wherein, all contains 3600 pixels in each test zone.Described relatively computing module is used for the pixel of the test zone of selecting is compared, and the concentration of quantitative Analysis sample; Described display is used to show the concentration results of sample correspondence.
Described speckle displacement determination module comprises regional setting module and position determination module; Described regional setting module is used for establishing a square area and set four rectangular areas around image in picture centre; The position of spot is determined in the requirement that described position determination module must cover described square area according to spot and can not enter any one described rectangular area.
Collaurum pick-up unit based on Digital Image Processing counts 0.5% through its survey read stability of test shows with CV, meet requirements for clinical application fully, and the CV of original U2 instrument is approximately 1%.
HCG serum specimen kit with the gold marking spot method is an example below, compares with regard to U2 instrument and the present invention.The low side detection line only can reach 100mIU on the U2 instrument, and the relative U2 instrument of low side sensing range of the present invention has extended to 10mIU.This is significant to clinical practice: because 25mIU is the negative positive critical point of HCG, because low side sensing range of the present invention has covered this point, therefore not only can realize the qualitative judgement of sample yin and yang attribute, can also realize quantitative application at low side.
In addition, the CRP project also gets a promotion along with low side detectability of the present invention, (sensing range: 5mg/L～160mg/L) can realize detection (scope: the 0.1mg/L～5mg/L) of super quick CRP again simultaneously to the detection that can realize conventional CRP in the present invention with the application extension of CRP on the U2 instrument.
Following table is the measurement range contrast of the present invention and U2 instrument.
Claims (7)
1. the collaurum detection method based on Digital Image Processing is characterized in that, may further comprise the steps:
(1) obtains the RGB coloured image of reaction plate;
(2) the RGB coloured image that obtains is converted into gray level image;
(3) utilize edge algorithms from gray level image, to extract the edge of target area to be analyzed;
(4) determine the center of spot in the image, distinguish central area and neighboring area;
(5) in central area and neighboring area, select four test zones respectively, and the pixel in the test zone is compared the concentration of quantitative Analysis sample.
2. the collaurum detection method based on Digital Image Processing according to claim 1 is characterized in that, is obtained the RGB coloured image of reaction plate in the described step (1) by the area array CCD detector, and described reaction plate is subjected to the irradiation of area source.
3. the collaurum detection method based on Digital Image Processing according to claim 1 is characterized in that, also comprises following substep in the described step (4):
(a) establish a square area in picture centre, the center of described square area is overlapped with the test paper center;
(b) around image, be provided with four rectangular areas;
(c) position of spot is determined in the requirement that must cover described square area according to spot and can not enter any one described rectangular area.
4. the collaurum detection method based on Digital Image Processing according to claim 1 is characterized in that, all contains 3600 pixels in each test zone of selecting in the described step (5).
5. device that uses the collaurum detection method based on Digital Image Processing as claimed in claim 1, comprise light source, detector, DSP image digitization processing platform and display, it is characterized in that described DSP image digitization processing platform comprises image conversion module, edge extracting module, speckle displacement determination module, zone selection module and the comparison computing module that links to each other successively; Described light source is an area source, with rayed on reaction plate; Described detector is the area array CCD detector, is used to obtain the RGB coloured image of reaction plate; Described image conversion module is used for the RGB coloured image is converted to gray level image; Described edge extracting module is used for extracting from gray level image the edge of target area to be analyzed; Described speckle displacement determination module is used for determining the center of image spot; Described zone selects module to be used for selecting four test zones respectively in speckle regions and neighboring area; Described relatively computing module is used for the pixel of the test zone of selecting is compared, and the concentration of quantitative Analysis sample; Described display is used to show the concentration results of sample correspondence.
6. use according to claim 5 is characterized in that based on the device of the collaurum detection method of Digital Image Processing described speckle displacement determination module comprises regional setting module and position determination module; Described regional setting module is used for establishing a square area and set four rectangular areas around image in picture centre; The position of spot is determined in the requirement that described position determination module must cover described square area according to spot and can not enter any one described rectangular area.
7. use according to claim 5 is characterized in that based on the device of the collaurum detection method of Digital Image Processing, all contains 3600 pixels in each test zone that described zone selects module to select.
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| CN102507565A (en) * | 2011-11-30 | 2012-06-20 | 刘江 | Method for searching reaction color development area of immunochromatographic strip |
| CN102692498A (en) * | 2012-06-11 | 2012-09-26 | 华中农业大学 | Automatic code reading device and automatic code reading method of sulfanilamide type medicine residue detecting reagent strip |
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| CN102507565A (en) * | 2011-11-30 | 2012-06-20 | 刘江 | Method for searching reaction color development area of immunochromatographic strip |
| CN102692498A (en) * | 2012-06-11 | 2012-09-26 | 华中农业大学 | Automatic code reading device and automatic code reading method of sulfanilamide type medicine residue detecting reagent strip |
| CN102692498B (en) * | 2012-06-11 | 2014-11-12 | 华中农业大学 | Automatic code reading device and automatic code reading method of sulfanilamide type medicine residue detecting reagent strip |
| CN102778455A (en) * | 2012-07-26 | 2012-11-14 | 杭州华光光电有限公司 | Detection system for colloidal gold immunochromatographic assay (GICA) test paper |
| CN102778455B (en) * | 2012-07-26 | 2014-12-10 | 杭州华光光电有限公司 | Detection system for colloidal gold immunochromatographic assay (GICA) test paper |
| CN103063663A (en) * | 2012-12-24 | 2013-04-24 | 大连大学 | Testing method for total phosphorus content in soil based on image analysis |
| CN103134568B (en) * | 2013-01-17 | 2015-09-30 | 厦门优迈科医学仪器有限公司 | A kind of detection method of liquid level and equipment thereof |
| CN103134568A (en) * | 2013-01-17 | 2013-06-05 | 厦门优迈科医学仪器有限公司 | Liquid level detection method and device thereof |
| CN104251861A (en) * | 2013-06-28 | 2014-12-31 | 成都谱视科技有限公司 | Intelligent terminal-based liquid detection analyzer and detection method using the same |
| CN103414944A (en) * | 2013-07-16 | 2013-11-27 | 深圳Tcl新技术有限公司 | Method and apparatus for fast previewing object file |
| CN104198482A (en) * | 2014-09-05 | 2014-12-10 | 北京智云达科技有限公司 | Test paper reading method and test paper reading device |
| CN105761262A (en) * | 2016-02-18 | 2016-07-13 | 马春阳 | Detection apparatus and detection method |
| CN105761262B (en) * | 2016-02-18 | 2018-10-16 | 马春阳 | A kind of detection device and detection method |
| CN106771169A (en) * | 2017-01-19 | 2017-05-31 | 厦门海荭兴仪器股份有限公司 | A kind of collaurum detection method and system based on image procossing |
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