CN101451948A - Quantitative determination device by gold marking spot method based on digital picture treatment - Google Patents
Quantitative determination device by gold marking spot method based on digital picture treatment Download PDFInfo
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- CN101451948A CN101451948A CNA2008102080289A CN200810208028A CN101451948A CN 101451948 A CN101451948 A CN 101451948A CN A2008102080289 A CNA2008102080289 A CN A2008102080289A CN 200810208028 A CN200810208028 A CN 200810208028A CN 101451948 A CN101451948 A CN 101451948A
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Abstract
The invention relates to a gold marking spotting method quantification detection equipment based on digital image processing, which is detection equipment based on a digital signal processor (DSP) and image processing technology, wherein a reaction plate image reading unit takes charge of reading reaction plate image, digital signals of the image are introduced to DSP, processed by the image process unit, corresponding concentration information to be measured is obtained, finally the information is output through a display unit. The invention is used in fast measurement of hormone content in humoral immunity of human body, concentration change of specific albumen, presence of pathogen or corresponding antibody, users can conveniently obtain concentration information of certain component to be measured in body fluid of certain human to be measured through simple operation, thereby delivering correct survey report to the doctor before the doctor makes decision, causing the most effective treatment on each specific patient.
Description
Technical field
The invention belongs to the human body fluid technical field of immunoassay, particularly relate to a kind of gold marking spot pick-up unit based on Digital Image Processing.
Background technology
Contain health whether important information in the body fluid, comprise that the existence of concentration change, pathogen or corresponding antibodies of height, specific protein of various hormone-contents and what etc., these project major parts to be measured can be detected with colloidal gold immunity percolation technology (gold marking spot method), testing result is that the observing response district has or not punctation to present, the punctation depth is directly proportional with the concentration of measured object, but only depend on the eyes can only qualitative interpretation, can't be quantitative.Lean against that the domestic light reflection technology of having used is quantitative, accuracy is not enough.
The colloidal gold immunity percolation technology is quick and simple and easy, can in several minutes, obtain testing result, training slightly, the nurse also can operate, and cooperates it quantitative if any the Digital Image Processing instrument, the quantitative reliably punctation depth, fast and easy gold marking spot method will become can be accurately quantitative the gold marking spot method, greatly enlarge its range of application, for example: the C-reactive protein (CRP) in blood plasma, serum or the whole blood is measured, and it is the important pointer that has or not bacterial infection.Reaction principle is a DASP sandwich method immunity test.Mix in reaction tube with the red collaurum conjugate of CRP monoclonal antibody testing sample dilution back, CRP combines with red collaurum conjugate specifically in the sample, then with the NC Nitroncellulose film in this potpourri adding and the kit reaction plate of flowing through, wherein contained, with the CRP of red conjugate combination, can for the CRP antibody specificity of solid phase on film catch, the spot that takes on a red color, spot red color intensity available digital pictures process instrumentation is measured, and CRP concentration is proportional in it and the sample.Now also there be not to find to have of the patent registration of CRP gold mark diafiltration digital picture quantitative technique in China.
The gold marking spot method has been widely used in the mensuration of human chorionic gonadal hormone (HCG) in serum or the urine, at present domestic relevant blood of human body hormone detects research and few, find through literature search prior art, Chinese patent title: multi-parameter household blood detector (patent No. 200510094052.0), this patent has been used MCU central controller and signal amplifier, and the blood sugar in the blood, uric acid and cholesterol are detected.Its usable range is wide, measurement is convenient though this invention is introduced, and its effect of telling also has certain gap from medical domain to the requirement of precision, computing velocity and instrument volume as a result.Simultaneously, the mensuration of hormone, specific protein etc. does not embody in this invention yet.In addition, the central processing unit that this invention is adopted is a single-chip microcomputer, does not adopt digital signal processor, does not also adopt the methods analyst of Digital Image Processing.
Summary of the invention
Technical matters to be solved by this invention provides a kind of gold marking spot pick-up unit based on digital signal processor (DSP) and image processing techniques, determine the content of certain important substance in the human body fluid immunity, the disease that the measured may suffer from is made quick diagnosis.Because used DSP and image processing techniques, whole device reached small and exquisite portable, computing is quick, the simple to operate and requirement of saving power consumption.
The technical solution adopted for the present invention to solve the technical problems is: a kind of quantitative determination device by gold marking spot method based on Digital Image Processing is provided, comprise colour imagery shot, digital signal processor and display screen, described pick-up unit comprises that also the reaction plate image reads in unit, graphics processing unit, result and shows pretreatment unit and display unit, wherein
(1) the reaction plate image reads in the unit and closely picks up the test paper image by high-resolution microspur colour imagery shot, reads in reaction zone image on the reaction plate, the picture signal of simulating is converted to digital signal imports digital signal processor DSP into;
(2) graphics processing unit is accepted to read in the digital signal that the unit imports digital signal processor DSP into from the reaction plate image, and reaction zone has the colouring information of testing concentration information on the calculating reaction plate, as a result of passes down;
(3) result shows that pretreatment unit accepts the result of importing into from graphics processing unit, has the approximate concentration value of determinand according to reaction zone on this value searching reaction plate, and these two values spread out of digital signal processor DSP with ad hoc fashion then, import subordinate into;
(4) described display unit is accepted the coordinate image analoging signal that digital signal processor DSP spreads out of, and directly is presented on the display screen.
The detection of described pick-up unit comprises that the existence and what the gold mark colour generation display analysis thereof of concentration change, pathogen or corresponding antibodies of height, the specific protein of the hormone-content of human body fluid immunity detects.
Image processing module in the described digital signal processor DSP can adopt the processor of various band image collecting functions.
Described graphics processing unit adopts the method for Digital Image Processing, image information to gold marking spot is carried out analyzing and processing, extraction has after the average brightness value of testing concentration information area, deducts the average brightness value of non-reaction zone on every side, to remove environmental impact.
Described result shows that pretreatment unit is converted to concentration information with mean flow rate Y value information and obtains by contrasting predefined colour brightness one testing concentration typical curve, from 0 to maximal value, search for the numerical value of AVEY axle successively, just write down corresponding concentration value after finding the AVEY value identical, as a result of be delivered to next stage with determinand.
Described display unit converts the testing concentration demonstration to by the mode of coordinate diagram.
The present invention adopts the method for Digital Image Processing, image information to gold marking spot is carried out analyzing and processing, extraction has after the average brightness value of hormone concentration information area, deduct the average brightness value of the test paper that does not have the blood contact on every side, to remove the neighbourhood noise influence.
Beneficial effect
The present invention has following advantage:
At first, DSP is as processing platform in utilization, can promote the speed of data processing greatly, and reaches light and handy portable purpose.
Secondly, the utilization image processing techniques with originally relying on the experience of human vision to judge the accurate Calculation that is converted to machine, not only improves accuracy, has also got rid of the standard inconsistency that causes because of individual differences.With respect to existing light reflection principle instrument on the Chinese market, has better resolution, particularly at low side and high-end good resolution arranged.
Once more, by displaing coordinate figure on display screen, can be in real time, directly, expression of results clearly, so just made things convenient for the user to read the result fast, in time draw the concentration of determinand.
Description of drawings
Fig. 1 is the structured flowchart of the principle of the invention.
Fig. 2 is the user mode figure of the embodiment of the invention.
Embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.Should be understood that in addition those skilled in the art can make various changes or modifications the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
As shown in Figure 1, 2, in the present embodiment, described device reads in unit, graphics processing unit, result by the reaction plate image and shows that pretreatment unit and display unit form, and wherein: analog to digital conversion, graphics processing unit, result that image reads in the unit show that the digital-to-analog conversion function of pretreatment unit and display unit is by the DSP Platform Implementation.Peripherals comprises colour imagery shot and display screen.
The reaction plate image reads in the unit and closely picks up the test paper image by high-resolution microspur colour imagery shot, guarantee that image is indeformable, color is undistorted, could guarantee the correctness of testing result like this.This simulating signal is imported among the DSP, at first arrives analog to digital converter and changes digital signal into.
Graphics processing unit receives and reads in the digital signal that the unit transmits from higher level's image, at first separates the brightness and the chrominance information of each pixel.Pick up the test paper center then and have 20*20 pixel in the hormone colouring information zone, calculate their average brightness value, 5*10 the pixel average brightness value that does not have the white portion of hormone colouring information around deducting again, the result who obtains can think approximate elimination environmental impact.
The result shows the average brightness value that pretreatment unit acceptance is transmitted from higher level's graphics processing unit, on (colour brightness-hormone concentration) typical curve, seek the hormone concentration of corresponding this value, a drop-down then perpendicular line is labeled in it on the concentration axle, and preparation shows jointly together with typical curve and coordinate axis on display screen.Simultaneously, prepare to show the numerical value of mean flow rate with dot matrix way.Organize after these information, by digital to analog converter digital signal is reduced to simulating signal and spreads out of DSP.Mean flow rate Y value information is converted to concentration information predefined by contrasting (colour brightness-hormone concentration) typical curve to be obtained, from 0 to maximal value, search for the numerical value of AVEY axle successively, just write down corresponding concentration value after finding the AVEY value identical, as a result of be delivered to next stage with test paper to be measured.
The simulating signal that display unit acceptance spreads out of from DSP, the coordinate diagram that shows a width of cloth white background black line by display screen, the drop-down perpendicular line that comprises coordinate axis, (colour brightness-hormone concentration) typical curve, one the hormone concentration size on the corresponding test paper to be measured from the typical curve on the figure, and the numerical value of mean flow rate.
The present invention is mainly used in following scene: on the reaction plate in reaction interval and the body fluid certain determinand reaction can cause the reaction interval change color, according to the brightness depth of this color, judge the concentration of this determinand.The user can judge disease of patient hidden danger or ill degree after obtaining the result, so that in time take corresponding measure.
Claims (6)
1. quantitative determination device by gold marking spot method based on Digital Image Processing, comprise colour imagery shot, digital signal processor and display screen, it is characterized in that, described pick-up unit comprises that also the reaction plate image reads in unit, graphics processing unit, result and shows pretreatment unit and display unit, wherein
(1) the reaction plate image reads in the unit and closely picks up the test paper image by high-resolution microspur colour imagery shot, reads in reaction zone image on the reaction plate, the picture signal of simulating is converted to digital signal imports digital signal processor DSP into;
(2) graphics processing unit is accepted to read in the digital signal that the unit imports digital signal processor DSP into from the reaction plate image, and reaction zone has the colouring information of testing concentration information on the calculating reaction plate, as a result of passes down;
(3) result shows that pretreatment unit accepts the result of importing into from graphics processing unit, has the approximate concentration value of determinand according to reaction zone on this value searching reaction plate, and these two values spread out of digital signal processor DSP with ad hoc fashion then, import subordinate into;
(4) described display unit is accepted the coordinate image analoging signal that digital signal processor DSP spreads out of, and directly is presented on the display screen.
2. a kind of quantitative determination device by gold marking spot method based on Digital Image Processing according to claim 1 is characterized in that: the human body fluid immune detection of described pick-up unit comprises that the existence and what the gold mark colour generation display analysis thereof of concentration change, pathogen or corresponding antibodies of height, the specific protein of the hormone-content of body fluid detects.
3. a kind of quantitative determination device by gold marking spot method based on Digital Image Processing according to claim 1 is characterized in that: the image processing module in the described digital signal processor DSP, can adopt the processor of various band image collecting functions.
4. a kind of quantitative determination device by gold marking spot method according to claim 1 based on Digital Image Processing, it is characterized in that: described graphics processing unit adopts the method for Digital Image Processing, image information to gold marking spot is carried out analyzing and processing, extraction has after the average brightness value of testing concentration information area, deduct the average brightness value of non-reaction zone on every side, to remove environmental impact.
5. a kind of quantitative determination device by gold marking spot method according to claim 1 based on Digital Image Processing, it is characterized in that: described result shows that pretreatment unit is converted to concentration information with mean flow rate Y value information and obtains by contrasting predefined colour brightness-testing concentration typical curve, from 0 to maximal value, search for the numerical value of AVEY axle successively, just write down corresponding concentration value after finding the AVEY value identical, as a result of be delivered to next stage with determinand.
6. a kind of quantitative determination device by gold marking spot method based on Digital Image Processing according to claim 1 is characterized in that: described display unit converts the testing concentration demonstration to by the mode of coordinate diagram.
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| CNA2008102080289A CN101451948A (en) | 2008-12-29 | 2008-12-29 | Quantitative determination device by gold marking spot method based on digital picture treatment |
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| CNA2008102080289A CN101451948A (en) | 2008-12-29 | 2008-12-29 | Quantitative determination device by gold marking spot method based on digital picture treatment |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102183510A (en) * | 2011-01-26 | 2011-09-14 | 上海奥普生物医药有限公司 | Colloidal gold detecting method and device based on digital image processing |
| CN103293112A (en) * | 2013-05-07 | 2013-09-11 | 北京航空航天大学 | In vitro allergen quantitative detector based on color detector and method of the detector |
| CN104251861A (en) * | 2013-06-28 | 2014-12-31 | 成都谱视科技有限公司 | Intelligent terminal-based liquid detection analyzer and detection method using the same |
| CN104969068A (en) * | 2012-08-08 | 2015-10-07 | 思勘度股份有限公司 | Method and apparatus for performing and quantifying color changes induced by specific concentrations of biological analytes in an automatically calibrated environment |
-
2008
- 2008-12-29 CN CNA2008102080289A patent/CN101451948A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102183510A (en) * | 2011-01-26 | 2011-09-14 | 上海奥普生物医药有限公司 | Colloidal gold detecting method and device based on digital image processing |
| CN102183510B (en) * | 2011-01-26 | 2012-11-14 | 上海奥普生物医药有限公司 | Colloidal gold detecting method and device based on digital image processing |
| CN104969068A (en) * | 2012-08-08 | 2015-10-07 | 思勘度股份有限公司 | Method and apparatus for performing and quantifying color changes induced by specific concentrations of biological analytes in an automatically calibrated environment |
| CN104969068B (en) * | 2012-08-08 | 2019-06-18 | 思勘度股份有限公司 | For the method and apparatus changed by the color that the biological analyte of certain concentration induces to be executed and quantified in automatic calibration environment |
| CN103293112A (en) * | 2013-05-07 | 2013-09-11 | 北京航空航天大学 | In vitro allergen quantitative detector based on color detector and method of the detector |
| CN104251861A (en) * | 2013-06-28 | 2014-12-31 | 成都谱视科技有限公司 | Intelligent terminal-based liquid detection analyzer and detection method using the same |
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