CN201184885Y - Automatic detection instrument for enzyme label micropore board - Google Patents
Automatic detection instrument for enzyme label micropore board Download PDFInfo
- Publication number
- CN201184885Y CN201184885Y CNU2007200757977U CN200720075797U CN201184885Y CN 201184885 Y CN201184885 Y CN 201184885Y CN U2007200757977 U CNU2007200757977 U CN U2007200757977U CN 200720075797 U CN200720075797 U CN 200720075797U CN 201184885 Y CN201184885 Y CN 201184885Y
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- micropore
- enzyme
- enzyme labeling
- camera system
- scanning platform
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Abstract
The utility model relates to an enzyme-labeled microplate automatic detector comprising an enzyme-labeled microplate combined array, the enzyme-labeled microplate automatic detector further comprises a two-dimensional rapid scanning platform, a light source, a spectroscope, a camera system and a computer, the enzyme-labeled microplate combined array is arranged on the two-dimensional rapid scanning platform, light emitted by the light source is radiated on the microplate combined array through the spectroscope, the computer can control the two-dimensional rapid scanning platform to be moved to the needed position, thereby allowing the camera system to point at a micropore, the camera system collects image data and stores in the computer; the enzyme-labeled microplate automatic detector can carry out rapid and automatic detection of an enzyme-labeled microplate, significantly improve the clinical diagnosis efficiency and the reliability of the enzyme-labeled microplate and have low equipment cost and strong practicality.
Description
Technical field
The utility model relates to a kind of Photobiology detecting instrument, particularly a kind of enzyme labeling microwell plate automatic tester.
Background technology
Enzyme labeling microwell plate diagnostic techniques is the diagnostic techniques that development in recent years is got up.Aspect clinical diagnosis, use enzyme mark technology usually, be produced on (being the enzyme labeling microwell plate) on the microwell plate, easy to use, safe.The basic process of enzyme linked immunosorbent assay is, the first step with purifying, identify the antigen coated on the solid phase polystyrene micropore plate of biochemical characteristic.If positive, the specific antibody in the serum that has diluted will combine with the antigen on being coated on solid phase; Second step, the anti-people's antibody and the antibody response that has combined of peroxidase labelling; The 3rd step, in conjunction with labelled antibody and the effect of chromogen substrate solution after and present color.The depth of color is directly proportional with the concentration of antibody in the serum specimen, can obtain for information about by detecting instrument.
But the automatic checkout equipment research that yet there are no special use reports that the enzyme labeling microwell plate can only detect or artificial qualitative evaluation by expensive microplate reader.Judge that by human eye vision its shortcoming has: can only qualitative analysis, can not detection by quantitative, precision is relatively poor, and testing staff's consitution difference can bring error, and detection speed is slow.It is very uneconomical to use microplate reader to detect, nor is beneficial to popularizing of clinical diagnosis biochip.
In addition, a plurality of probes of point sample in each micropore as viral probes such as while point sample hepatitis A, hepatitis B, third liver in a micropore, then only need a small amount of blood sample can once finish various hepatitis viruss and detect.Present microplate reader requires can only add a kind of probe in each micropore owing to do not adopt imaging technique.Desire is carried out multinomial virus to a measured and is detected, and can only carry out respectively at a plurality of micropores, needs to extract more blood sample, detects demand status from a large amount of patients, and detection efficiency is very low.Only,, therefore press for the special-purpose enzyme labeling microwell plate automatic checkout system of exploitation at present owing to the ratio of carrying various hepatitis viruss in China's population is very high from aspect of hepatitis virus.
Summary of the invention
The utility model be at existing can only qualitative analysis, can not detection by quantitative, precision is relatively poor, detection speed is slow, be to cause the problem of error to propose a kind of enzyme labeling microwell plate automatic tester easily by the people, employing to each micropore respectively imaging method obtain the method for signal, can realize diagnosis different virus, disease the time in each micropore is improved detection efficiency and reliability greatly.
The technical solution of the utility model is: a kind of enzyme labeling microwell plate automatic tester, comprise enzyme labeling micropore combination array, it is characterized in that also comprising two dimension scanning platform, light source, spectroscope, camera system, computing machine fast, enzyme labeling micropore combination array places on the quick scanning platform of two dimension, the light that light source sends shines on the micropore combination array by spectroscope, computer-controllable system two dimension scanning platform fast moves to desired location, make camera system aim at micropore, camera system is gathered pictorial data and is saved in the computing machine.12 micropore unit of the every row of described enzyme labeling micropore combination array, 96 micropore unit of totally 8 rows form 12 * 8 micropore combination array.Can import and remember the relative position of described enzyme labeling micropore combination array in the described computing machine.
To each micropore respectively imaging method obtain the method for signal, can realize a plurality of probes of point sample in each micropore, diagnosis to different virus, disease the time, improved detection efficiency greatly.Use the dynamo-electric scanning system of high speed two-dimension to drive 96 hole microwell plates and move, use laser lighting simultaneously, respectively to each micro-pore imaging, and save as image file respectively, handle with process software then, provide testing result by imaging and photoelectric device.
The beneficial effects of the utility model are enzyme labeling microwell plate automatic tester, adopt ray machine electricity, computer technology to combine, can detect fast and automatically, significantly improve enzyme labeling microwell plate clinical diagnosis efficient and reliability the enzyme labeling microwell plate, equipment cost is low, and is practical.
Description of drawings
Fig. 1 is single micropore of the utility model and dot matrix synoptic diagram;
Fig. 2 is a plurality of micropore combination array of a utility model synoptic diagram;
Fig. 3 is the utility model enzyme mark microwell plate fast detecting instrument schematic diagram.
Embodiment
As single micropore of Fig. 1 and dot matrix synoptic diagram, A is a dot matrix, and B is a tube wall, with the single array format that is arranged in as shown in Figure 2, and 12 micropore unit of every row, 96 micropore unit of totally 8 rows form 12 * 8 micropore combination array.
The enzyme labeling micropore combination array that desire is detected places on the quick scanning platform 2 of two dimension.The light that light source 3 sends shines on the enzyme labeling micropore combination array by spectroscope 4; Adjust two dimension fast scanning platform 2 make 5 pairs of the 1st micropores of camera system (being positioned at first micropore that ranked first row) to initial position, as datum hole, and gather pictorial data and be saved in the computing machine 6.Then, two dimension scanning platform 2 drive micropore combination array under computing machine 6 controls fast moves a micropore at interval in the x direction, makes 5 pairs of the 2nd micro-pore imagings of camera system and saves image data; Continuation is to first other micro-pore imaging of row and the image data.After first row's micropore detected and finishes, two dimension scanning platform 2 fast moved a micropore at interval in the y direction, began the 2nd row's micropore is detected.Finish up to all 96 micropore unit detections.
After image acquisition is finished, the pictorial data of each micropore is handled and analyzed, provide evaluation result by computer software.
Claims (3)
1, a kind of enzyme labeling microwell plate automatic tester, comprise enzyme labeling micropore combination array, it is characterized in that also comprising two dimension scanning platform, light source, spectroscope, camera system, computing machine fast, enzyme labeling micropore combination array places on the quick scanning platform of two dimension, the light that light source sends shines on the micropore combination array by spectroscope, computer-controllable system two dimension scanning platform fast moves to desired location, make camera system aim at micropore, camera system is gathered pictorial data and is saved in the computing machine.
2, according to the described enzyme labeling microwell plate of claim 1 automatic tester, 12 micropore unit of the every row of described enzyme labeling micropore combination array, 96 micropore unit of totally 8 rows form 12 * 8 micropore combination array.
3,, can import and remember the relative position of described enzyme labeling micropore combination array in the described computing machine according to the described enzyme labeling microwell plate of claim 1 automatic tester.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2007200757977U CN201184885Y (en) | 2007-11-21 | 2007-11-21 | Automatic detection instrument for enzyme label micropore board |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2007200757977U CN201184885Y (en) | 2007-11-21 | 2007-11-21 | Automatic detection instrument for enzyme label micropore board |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201184885Y true CN201184885Y (en) | 2009-01-21 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2007200757977U Expired - Fee Related CN201184885Y (en) | 2007-11-21 | 2007-11-21 | Automatic detection instrument for enzyme label micropore board |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201184885Y (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104198742A (en) * | 2014-08-20 | 2014-12-10 | 曲阜裕隆生物科技有限公司 | Standard machine CCD setting method-enzyme hole marking method |
| CN108519332A (en) * | 2018-04-28 | 2018-09-11 | 南通市肺科医院（南通市第六人民医院） | Full-automatic microwell plate system and the computer-readable medium for the system |
| CN113552337A (en) * | 2021-07-19 | 2021-10-26 | 中山大学 | Full-spectrum high-efficiency microplate reader system |
-
2007
- 2007-11-21 CN CNU2007200757977U patent/CN201184885Y/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104198742A (en) * | 2014-08-20 | 2014-12-10 | 曲阜裕隆生物科技有限公司 | Standard machine CCD setting method-enzyme hole marking method |
| CN108519332A (en) * | 2018-04-28 | 2018-09-11 | 南通市肺科医院（南通市第六人民医院） | Full-automatic microwell plate system and the computer-readable medium for the system |
| CN108519332B (en) * | 2018-04-28 | 2019-12-03 | 南通市肺科医院（南通市第六人民医院） | Full-automatic microwell plate system and the computer-readable medium for the system |
| CN113552337A (en) * | 2021-07-19 | 2021-10-26 | 中山大学 | Full-spectrum high-efficiency microplate reader system |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090121 Termination date: 20091221 |