CN102138434A - Method for producing black fungus hypha rich in selenium - Google Patents

Abstract

The invention discloses a method for producing black fungus hypha rich in selenium, wherein the test-tube black fungus seed or special liquid seed of black fungus after inoculation and propagation is inoculated into a culture solution with a reasonable carbon/nitrogen ratio in a fermentation tank for aerobic fermentation; a proper amount of sodium selenite or sodium selenate is dissolved in the culture solution in the fermentation tank; the temperature is controlled at 22-32 DEG C; 48-120 hours later, the culture solution in the fermentation tank is filtered to obtain the black fungus hypha rich in selenium; the black fungus hypha is dried and ground to obtain the dry powder of black fungus.

Description

The abundant woodear mycelia production method that contains selenium

Technical field

The present invention relates to a kind of hypha of edible fungus production method.Relate in particular to a kind of abundant production method that contains the woodear mycelia of selenium, the microorganism belonging to genus field.

Background technology

Selenium is the necessary a kind of trace element of human body, and scarce selenium can cause the multiple disease of human body, and China overwhelming majority area lacks selenium, quantitatively mends selenium, and is very urgent.The scope of taking in inorganic selenium because of human body safety every day is narrower, and human body is bigger to the tolerance degree of organic selenium, is absorption of human body again after changing inorganic selenium into organic selenium, is the best approach of human body safe selenium-complement.Woodear has very strong transformation inorganic selenium in process of growth be the ability of organic selenium, and especially at vegetative stage, mycelia has the extremely strong ability of organic selenium that inorganic selenium is converted into, and contain the abundant polysaccharide that is beneficial to human health.Yet, regrettably: at present in the society not can easily, environmental protection, the more stable woodear mycelia of large batch of production organic selenium content.

The invention has the beneficial effects as follows: can easily, environmental protection, the more stable woodear mycelia of large batch of production organic selenium content, add in the various F﹠B, be convenient to people and quantitatively mend selenium.

Summary of the invention

Present known woodear mycelia procurement process is: several links such as solid culture medium spice, pack, sterilization, inoculation, bacteria, pulverizing, filtration, extraction, drying.The invention provides and a kind ofly need not plant woodear, only the woodear bacterial classification is cultivated the mycelia stage and can be gathered in the crops the abundant production method that contains the woodear mycelia of selenium, the technical scheme that realizes is to adopt the test tube woodear kind of no living contaminants, it is inoculated in the rational culture fluid of C/N ratio, carry out conventional bottle, the constant temperature culture of shaking, expand numerous after, it is inoculated into is contained in the fermentation tank, carries out aerobic fermentation in the rational culture fluid of C/N ratio; Also can select woodear liquid specific kind for use, directly be inoculated into and be contained in the fermentation tank, carry out aerobic fermentation in the rational culture fluid of C/N ratio, dissolve in an amount of sodium selenite or sodium selenate in the culture fluid in above-mentioned fermentation tank, the concentration of sodium selenite or sodium selenate is less than every liter of 1 gram, the size of this concentration has determined the selenium concentration that contains of the woodear mycelia turned out, temperature is controlled at 22 to 32 degrees centigrade, last 48 hours after 120 hours, filter the culture fluid in the fermentation tank, the abundant woodear mycelia that contains selenium can be obtained, woodear mycelia dry powder can be obtained behind the dry abrasive dust.When aerobic fermentation finished, the culture fluid in the fermentation tank was as bacteria infection not, then before filtering, got the inoculation seed that culture fluid that a part comprises a large amount of woodear mycelia can be used as next jar, carried out the cultivation of next jar woodear mycelia.

Specific embodiment

Get 90Kg water and the 9Kg potato boils to shortcake, add 1.35Kg brown sugar, 0.9Kg glucose, 3.6Kg wheat bran, 0.18Kg peptone, 0.18Kg potassium dihydrogen phosphate, 0.09Kg magnesium sulfate, 0.9g vitamin V B1,27 milliliters defoamer, filter the back woodear mycelia culture fluid, through sterilization, behind the adding 6 gram sodium selenites, woodear liquid specific kind is poured in the culture fluid, carrying out aerobic cultivates, the control temperature is 25 degrees centigrade, lasts 72 hours, culture fluid is filtered promptly to get enrich the woodear mycelia that contains selenium.

Claims (1)

1. abundant production method that contains the woodear mycelia of selenium, it is characterized in that: through inoculation, expand test tube woodear kind or woodear liquid specific kind after numerous, be inoculated into and be contained in the fermentation tank, carry out aerobic fermentation in the rational culture fluid of C/N ratio, dissolved in an amount of sodium selenite or sodium selenate in the culture fluid in the fermentation tank, the concentration of sodium selenite or sodium selenate is less than every liter of 1 gram.