CN102132671A

CN102132671A - Method for secondary cultivation of shell conchospores of porphyra haitanensis

Info

Publication number: CN102132671A
Application number: CN 201010585465
Authority: CN
Inventors: 刘长军; 尹根卫; 郑岳夫
Original assignee: XIANGSHAN TAIKONG HONGZICAI GROW-SEEDING STATION; XIANGSHAN AQUATIC PRODUCT TECHNOLOGY EXTENSION STATION
Current assignee: XIANGSHAN TAIKONG HONGZICAI GROW-SEEDING STATION; XIANGSHAN AQUATIC PRODUCT TECHNOLOGY EXTENSION STATION
Priority date: 2010-12-02
Filing date: 2010-12-02
Publication date: 2011-07-27
Anticipated expiration: 2030-12-02
Also published as: CN102132671B

Abstract

The invention discloses a method for the secondary cultivation of shell conchospores of porphyra haitanensis, which comprises the following steps of: recultivating shells obtained after the shell conchospores are diffused for the first time in a cultivating pond with water depth of between 20 and 30 centimeters at the water temperature of between 28 and 30 DEG C for 13 to 30 days; adding monopotassium phosphate in the cultivating process; performing light reducing in the cultivating process for 7 to 8 hours each day for 5 to 8 days, and controlling the illumination intensity to be between 800 and 10,001x; performing microscopic examination on shell protonema obtained by cultivation, and when dual-differential cells are over 80 percent of integral conchospores, diffusing the conchospores; and diffusing the conchospores and attaching a net in the sea at the temperature of less than or equal to 27 DEG C. In the method, due to secondary utilization on the shells of which the conchospores are diffused, the conchospores are matured and diffused for the second time by concentrated cultivating measures, and secondary large-scale seedlings are provided for production to achieve the effects of high and stable yield; and the capacity of cultivating the porphyra haitanensis is improved, and the production cost is reduced.

Description

Porphyra haitanensis shell conchospore secondary breeding method

Technical field

The present invention relates to the cultural technique field of seaweeds, especially relate to a kind of Porphyra haitanensis shell conchospore secondary breeding method.

Technical field

Porphyra haitanensis, popular name laver, black dish.Porphyra haitanensis is a kind of dried marine products of cheap and tasty, instant, and laver mainly contains southern Porphyra haitanensis and northern porphyra yezoensis two big classes.Porphyra haitanensis mainly is distributed in coastal state such as China Zhejiang, Fujian, is the peculiar kind of China, has fast, the characteristics such as output is high, raciness of growth.Nutriments such as Porphyra haitanensis rich in proteins, vitamin A and iodine, phosphorus, calcium, wherein the height of protein and vitamin content is rare in tangleweed, is the treasure in the marine alga, have the good name of " long-life vegetable ", " angle's dish ", liked by the consumer.Along with the expansion in market, the major production areas of Porphyra haitanensis gives enough attention to the development Porphyra haitanensis, has innovated many good breed new technologies.

The growth of Porphyra haitanensis mainly is two tangible developmental stage of branch, the one, and the shell conchocelis breeding phase, the 2nd, thallus is cultured the stage.Shell conchocelis is to be sprouted by the carpospore that thallus diffuses out to form, and majority is grown in shell; Thallus is to be sprouted by the conchospore that filamentous diffuses out to form promptly daily edible laver.Growing seedlings of laver just is meant the carpospore that forms after the thallus maturation, cultivates shell conchocelis by the fruit picking spore, the process that filamentous diffuses conchospore after the maturation in the fall.

Wherein the shell conchocelis breeding phase is particularly important, is mainly: 1. fruit picking spore or pick up from by filamentous: (1), diffuse carpospore, with planting vegetables of using, in Xiao Chi, diffuse carpospore through putting in a suitable place to breed behind the dry in the shade stimulation, the process of diffusing constantly stirs seawater; (2), collect carpospore, diffuse the back and spore fluid carried out filtering and concentrating with 4 layers～5 layers gauze, draw the quantity that the water sample inspection is diffused, sampling counting back collection carpospore; (3), collect seedling or be the fruit picking spore, in advance the clam shell is hung in the pond that is marked with the precipitation seawater, (throw in the density grasp according to carpospore and adhere to 200～300 spores at every square metre of shell face, calculate spore amount required on the Seedling culturing device of every pond) get and concentrate spore fluid and be sprayed on the clam shell after with the seawater dilution, spraying the back stirs in the middle of the space, carpospore is evenly distributed and is attached to shell surface, the end of collecting seedling; Perhaps, will through the laver free conchocelis that spreading cultivation in the laboratory with picking up from by the filamentous method, after with small-sized beating crusher free conchocelis being smashed, transplanting amount 100mg/m ²More than attached to shell surface, the end of collecting seedling; 2. seed rearing, collect seedling and carried out scrubbing the first time shell in back about 15 days and change water, later per two weeks scrub once, use the banister brush fine laundering, in order to avoid damage shell face destroys algal filament, notice when scrubbing that shell can not do dew, and according to different times control intensity of illumination and temperature, salt etc. supplements the nutrients in time, shell surface grows filamentous, treat that the filamentous maturation becomes and expand algal filament,, can enter next process by expanding after the algal filament regrowth forms the conchospore branch; 3. diffuse conchospore or cry and adopt conchospore, diffuse conchospore and be actually and transfer to the course of work on the nylon netting curtain adhering to conchospore that shell surface forms.

Annual by the end of August at the beginning of 9 months, region following the line of the sea, Zhejiang or typhoon or heavy showers influence all have one section temperature-fall period, laver conchospore maturation is diffused and favourable, thereby the raiser can concentrate the stimulation in the sea shell to impel conchospore to diffuse during this period of time, and then the cultivation of plunging into the commercial sea.Yet, because global climate is unusual in recent years, temperature fall time continues shorter, after plunging into the commercial sea Deng the laver lace curtaining, continue water temperature 29.5～30 ℃ nearly 20 days, so high water temperature makes and comes off easily behind the attached net of conchospore or claims to fall seedling, is the abortive haul of no conchospore on the lace curtaining, net in vain thereby cause a large amount of laver lace curtainings " white net ", " abortive haul " phenomenon to occur.After but the shell conchospore diffuses, generally shell is directly thrown away, can't be that " white net ", " abortive haul " provide seed again, thereby have a strong impact on the output of laver, raiser's loss be bigger.And, also have on the shell of throwing away and do not diffuse about 20% conchospore completely, if directly abandon, will cause a large amount of wastes of seedling.

Summary of the invention

The present invention is directed to the above-mentioned deficiency of prior art, providing a kind of can provide the secondary conchospore to shift for laver lace curtaining " white net ", " abortive haul ", effectively guarantee laver output, reduce raiser's loss, make full use of the Porphyra haitanensis shell conchospore secondary breeding method of shell conchospore.

In order to solve the problems of the technologies described above, technical scheme of the present invention is: a kind of Porphyra haitanensis shell conchospore secondary breeding method, and technical process is as follows: and the shell after the shell conchospore diffuses for the first time lays again → applies fertilizer → and the light 5-8 days → microscopy → shell stimulation in the sea → conchospore that contracts diffuses → the attached lace curtaining → cultivation of plunging into the commercial sea;

Detailed process is as follows:

(1) gets shell after the shell conchospore diffuses for the first time, it is laid on cultivates the bottom, pond and cultivate;

(2) cultivate the seawater that adds the depth of water 20～30cm in the pond, water temperature is 28～30 ℃, 13～30 days cultivation time;

(3) cultivating process adding potassium dihydrogen phosphate applies fertilizer, and makes that its concentration in cultivating the pond seawater is 5～10ppm, fertilising in per 10 days 1 time;

(4) the cultivating process light that contracts is handled, and contracts between the light time 5～8 days, and 7～8 hours every days, intensity of illumination is controlled at 800～1000lx (lux);

(5) get the shell conchocelis of cultivating gained and carry out microscopy,, can diffuse conchospore when two minutes cells account for more than 80% of conchospore;

(6) in ocean temperature≤27 ℃, with the conchospore attached net that diffuses, plunges into the commercial sea.

The present invention cultivate in the pond seawater for through obscure shallow lake more than 3 days again through the seawater of sand filtration.

The light that contracts that the present invention is above-mentioned is for forming the phase at the microscopy shell conchocelis at cystite later stage, two minutes cell, and the light that begins to contract is handled.

The above-mentioned shell of the present invention is the clam shell.

Advantage of the present invention and beneficial effect:

1. the shell after the present invention mainly takes conchospore diffused carries out second use at once, on the one hand can be by concentrating cultural operations, impel the conchospore maturation second time and diffuse,, thereby reach the effect of stable yields and high yield for production provides the seed of scale for the second time; On the other hand, improved the production capacity of culturing Porphyra haitanensis, reduced production costs.

2. laver shell conchospore secondary is cultivated the technology strong operability, and method is simple, and promotional value is big.

3. the conchospore secondary is cultivated and is solved because " white net ", " abortive haul " phenomenon that factors such as weather cause makes " white net ", " abortive haul " obtain the attached net of secondary of conchospore, improves attached net rate.Thereby can stablize provides seed, does not delay and cultures season, reduces the waste loss of seedling.

4. emerge when the seawater water temperature is equal to or less than 27 ℃, be easy to conchospore and concentrate, conchospore diffuses the rate height, attached net rate height.

Embodiment

The invention is further illustrated by the following examples, but the present invention not only is confined to following examples.

Embodiment is as follows:

(1) gets shell (clam shell) after the shell conchospore diffuses for the first time, it closely is tiled in cultivates the bottom, pond;

(2) cultivate in the pond and be marked with the dark seawater of 20～30cm, 28～30 ℃ of water temperatures, the cultivation time: 15 days, seawater be through obscure shallow lake more than 3 days again through the seawater of sand filtration;

(3) cultivating process applies fertilizer simultaneously, promptly executes potassium dihydrogen phosphate, and making its concentration in the seawater of cultivating the pond is 5ppm, fertilising in 10 days 1 time; Change water according to the water quality situation about 7～8 days growing seedlings, quantity of exchanged water is generally 5～10cm degree of depth;

(4) contract between the light time: the filamentous that grows on microscopic examination (microscopy) shell, it is in cystite later stage, two minutes cell and forms the phase, and the light that begins to contract was handled 5～8 days, and 7～8 hours every days, intensity of illumination is controlled at 800～1000lx;

(5) microscopy filamentous, the two cells that divide of thread topic bud into account for more than 80% of conchospore, can plunge into the commercial sea and diffuse;

(6) conchospore diffuse, attached net, the cultivation of plunging into the commercial sea: the conchospore with maturation during in ocean temperature≤27 ℃ diffuses, and is specially the industry routine techniques: in the culturing area of sea area, set up culture raft frame in advance.Elder generation is with one night of shell stimulation in the sea of maturation, shell was taken bank in second day, put into prior bottom and spread the pond of collecting seedling of gas ducting, simultaneously nylon netting curtain is put into the pond of collecting seedling, and open air pump formation current, stimulate the conchospore in the shell to diffuse the back, after reaching the breed quantity of plunging into the commercial sea behind the microscopy attached on the lace curtaining, the lace curtaining of attached full conchospore is hung on the culture raft frame of sea area, culture the stage thereby enter the sea area.

Claims

1. Porphyra haitanensis shell conchospore secondary breeding method, it is characterized in that: incubation step is:

(3) cultivating process adds potassium dihydrogen phosphate, 5～10ppm, fertilising in per 10 days 1 time;

(4) the cultivating process light that contracts is handled, and contracts between the light time 5～8 days, and 7～8 hours every days, intensity of illumination is controlled at 800～1000lx;

(5) get the shell conchocelis of cultivating gained and carry out microscopy,, can diffuse conchospore when two minutes cells account for more than 80% of whole conchospore;

2. Porphyra haitanensis shell conchospore secondary breeding method according to claim 1 is characterized in that: the seawater in the described cultivation pond for through obscure shallow lake more than 3 days again through the seawater of sand filtration.

3. Porphyra haitanensis shell conchospore secondary breeding method according to claim 1 is characterized in that: the described light that contracts is for handling at cystite later stage, the two minutes cell formation phase light that begins to contract at the microscopy shell conchocelis.

4. Porphyra haitanensis shell conchospore secondary breeding method according to claim 1, it is characterized in that: described shell is the clam shell.