CN102119596B - Seed soaking processing method for simultaneously improving cold and drought resistance of tobacco seed - Google Patents
Seed soaking processing method for simultaneously improving cold and drought resistance of tobacco seed Download PDFInfo
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- CN102119596B CN102119596B CN 201110031537 CN201110031537A CN102119596B CN 102119596 B CN102119596 B CN 102119596B CN 201110031537 CN201110031537 CN 201110031537 CN 201110031537 A CN201110031537 A CN 201110031537A CN 102119596 B CN102119596 B CN 102119596B
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- 238000002791 soaking Methods 0.000 title claims abstract description 59
- 241000208125 Nicotiana Species 0.000 title claims abstract description 24
- 235000002637 Nicotiana tabacum Nutrition 0.000 title claims abstract description 24
- 238000003672 processing method Methods 0.000 title abstract description 5
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims abstract description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 24
- 239000012153 distilled water Substances 0.000 claims abstract description 15
- 238000000034 method Methods 0.000 claims abstract description 8
- 238000004659 sterilization and disinfection Methods 0.000 claims description 8
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 239000007788 liquid Substances 0.000 abstract description 4
- 239000002352 surface water Substances 0.000 abstract 2
- 239000005708 Sodium hypochlorite Substances 0.000 abstract 1
- 238000001035 drying Methods 0.000 abstract 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 230000035784 germination Effects 0.000 description 35
- 229910001628 calcium chloride Inorganic materials 0.000 description 18
- 239000001110 calcium chloride Substances 0.000 description 18
- 239000003814 drug Substances 0.000 description 16
- 235000003255 Carthamus tinctorius Nutrition 0.000 description 12
- 244000020518 Carthamus tinctorius Species 0.000 description 12
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 12
- 239000010931 gold Substances 0.000 description 12
- 229910052737 gold Inorganic materials 0.000 description 12
- 238000005286 illumination Methods 0.000 description 6
- 230000002411 adverse Effects 0.000 description 5
- 230000008641 drought stress Effects 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 4
- 235000019504 cigarettes Nutrition 0.000 description 4
- 230000003203 everyday effect Effects 0.000 description 4
- 238000010899 nucleation Methods 0.000 description 4
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 230000007226 seed germination Effects 0.000 description 3
- 230000035882 stress Effects 0.000 description 3
- JLIDBLDQVAYHNE-YKALOCIXSA-N (+)-Abscisic acid Chemical compound OC(=O)/C=C(/C)\C=C\[C@@]1(O)C(C)=CC(=O)CC1(C)C JLIDBLDQVAYHNE-YKALOCIXSA-N 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 229920001223 polyethylene glycol Polymers 0.000 description 2
- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000006735 deficit Effects 0.000 description 1
- FCRACOPGPMPSHN-UHFFFAOYSA-N desoxyabscisic acid Natural products OC(=O)C=C(C)C=CC1C(C)=CC(=O)CC1(C)C FCRACOPGPMPSHN-UHFFFAOYSA-N 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- ULWHHBHJGPPBCO-UHFFFAOYSA-N propane-1,1-diol Chemical class CCC(O)O ULWHHBHJGPPBCO-UHFFFAOYSA-N 0.000 description 1
- 229960004889 salicylic acid Drugs 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- YNWVFADWVLCOPU-MAUPQMMJSA-N uniconazole P Chemical compound C1=NC=NN1/C([C@@H](O)C(C)(C)C)=C/C1=CC=C(Cl)C=C1 YNWVFADWVLCOPU-MAUPQMMJSA-N 0.000 description 1
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- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses seed soaking liquid and a seed soaking method for simultaneously improving the cold and drought resistance of a tobacco seed. The used seed soaking liquid is a 5-10 mg/L calcium chloride solution; the seed soaking condition comprises dark seed soaking at 20-30 DEG C for 12-24 hours; after the seed soaking is finished, the seed is rapidly washed with distilled water; and after the surface water is completely sucked, the seed is placed at 25-30 DEGC for dying to the original water content. Before the seed is soaked, the seed is disinfected with a 0.5-2 percent sodium hypochlorite solution for 10-20 minutes; then the seed is rapidly washed with the distilled water; and after the surface water is completely sucked, the seed is arranged at 25-30DEG C for drying to the original water content. The seed soaking chemical used by the invention has low price, the seed soaking method is simple and easy, and the invention provides ideal seed soaking liquid and a seed soaking processing method.
Description
Technical field
The invention belongs to the seed processing technology in the agricultural technology field, be specifically related to a kind of cold-resistant seed-soaking method with drought-resistant ability of tobacco seed that improves simultaneously.
Background technology
Tobacco is the warm crop of happiness, and is relatively poor to the low temperature stress tolerance.Chilling injury is the natural calamity that often runs on tobacco produces, and is one of factor of restriction leaf tobacco production.Simultaneously, tobacco originates from the abundant torrid zone of rainfall, and is also very high to the requirement of moisture.And China part leaf tobacco production district is in the mountain area, hills, and not only there is the problem of low temperature harm in these areas, and owing to reasons such as landform, soil water deficit occurs easily and forms the arid infringement, has influenced the growth of local cigarette seedling.Therefore, improve tobacco seed through the seed soaking process etc. techniques and seedling is cold-resistant, drought-resistant ability has important practical significance.
At present; Handle the resistance that improves seed through seed soaking and on many crops, be applied, still, the seed soaking medicament is often to single adverse circumstance screening; Obtain corresponding cold-resistant seed soaking medicament or drought-resistance seed-soaking medicament, and there is the problem of low temperature and drought stress simultaneously in some cigarette districts of China.Therefore, this seed soaking medicament that filters out to single adverse circumstance is difficult to satisfy the needs in these cigarette districts.Simultaneously; Because changing, the Physiology and biochemistry that seed is taken place has similitude under low temperature, drought stress; And drought resisting, its principle of cold tolerance of improving seed through the medicament seed soaking are being consistent aspect some; As improve cell membrane stability, and improving the activity of antioxidase, the content that improves soluble sugar in the cell waits to reach and improves seed and seedling resistance purpose.So, filter out through correlation test and can improve simultaneously that tobacco seed is cold-resistant, the seed soaking medicament of drought-resistant ability is feasible in theory.The present invention is based on above imagination; A large amount of tests have been carried out; Test through different medicament variable concentrations different times; From medicaments such as proline, salicylic acid, calcium chloride, glycerine, dimethyl sulfoxide (DMSO), ethylene glycol, polyethylene glycol, propane diols, uniconazole P, abscisic acid, filter out seed soaking medicament and seed soaking concentration and the time that to improve the cold-resistant and drought-resistant ability of tobacco seed simultaneously, can satisfy the needs that the cigarette district produces.
Summary of the invention
The objective of the invention is to the medicament screening of soaking seed in the past only according to this deficiencies of single adverse circumstance such as arid or low temperature, a kind of cold-resistant seed soaking processing method with drought-resistant ability of tobacco seed that improves simultaneously is provided.
The object of the invention is realized through following technical scheme:
Improve simultaneously tobacco seed cold-resistant with seed-soaking method drought-resistant ability be: at first; Use mass percent concentration to be 0.5-2% liquor natrii hypochloritis disinfection seed 10-20 min; Sterilization back is got seed express developed with distilled water, under 25-30 ℃, is dried to initial moisture content after blotting the surface of the seed moisture; Then, seed is placed the calcium chloride solution of 5-10 mg/L, 20-30 ℃ of dark down seed soaking 12-24 h gets seed express developed with distilled water at last, blots the surface of the seed moisture and is placed on and is dried to initial moisture content under 25-30 ℃, stores for use.
The beneficial effect that the present invention compared with prior art has:
1) the seed soaking medicament that is screened can improve the cold-resistant and drought-resistant ability of tobacco seed simultaneously;
2) employed medicament low price, seed-soaking method is simple, is desirable seed-soaking liquid and seed soaking processing method.
Embodiment:
Embodiment 1
At first, get the big gold dollar of safflower and each 2g of Msk326 seed, using mass percent concentration is 0.5% liquor natrii hypochloritis, 10 min that sterilize, and the sterilization back is got seed express developed with distilled water, under 25 ℃, is dried to initial moisture content after blotting the surface of the seed moisture; Then, seed is placed the calcium chloride solution of 5 mg/L, 20 ℃ of dark down seed soaking 12 h; Get seed express developed with distilled water at last; Blot the surface of the seed moisture and be placed under 25 ℃ and be dried to initial moisture content, the tobacco seed after obtaining handling can be used for seeding and seedling raising.
Embodiment 2
At first, get the big gold dollar of safflower and each 2g of Msk326 seed, using mass percent concentration is 2% liquor natrii hypochloritis, 20 min that sterilize, and the sterilization back is got seed express developed with distilled water, under 30 ℃, is dried to initial moisture content after blotting the surface of the seed moisture; Then, seed is placed the calcium chloride solution of 10 mg/L, 30 ℃ of dark down seed soaking 24 h; Get seed express developed with distilled water at last; Blot the surface of the seed moisture and be placed under 30 ℃ and be dried to initial moisture content, the tobacco seed after obtaining handling can be used for seeding and seedling raising.
Embodiment 3
At first, get the big gold dollar of safflower and each 2g of Msk326 seed, using mass percent concentration is 1% liquor natrii hypochloritis, 15 min that sterilize, and the sterilization back is got seed express developed with distilled water, under 30 ℃, is dried to initial moisture content after blotting the surface of the seed moisture; Then, seed is placed the calcium chloride solution of 7.5 mg/L, 25 ℃ of dark down seed soaking 12 h; Get seed express developed with distilled water at last; Blot the surface of the seed moisture and be placed under 25 ℃ and be dried to initial moisture content, the tobacco seed after obtaining handling can be used for seeding and seedling raising.
Embodiment 4
At first, get the big gold dollar of safflower and each 2g of Msk326 seed, using mass percent concentration is 1% liquor natrii hypochloritis, 15 min that sterilize, and the sterilization back is got seed express developed with distilled water, under 30 ℃, is dried to initial moisture content after blotting the surface of the seed moisture; Then, seed is placed the calcium chloride solution of 7.5 mg/L, 30 ℃ of dark down seed soaking 24 h; Get seed express developed with distilled water at last; Blot the surface of the seed moisture and be placed under 30 ℃ and be dried to initial moisture content, the tobacco seed after obtaining handling can be used for seeding and seedling raising.
Application example 1
Embodiment 1 is handled the back seed as experiment material with embodiment 2 gained, be respectively contrast 1 and 2 (CK1 and CK2) with dry seeds and clear water seed soaking and carry out indoor germination test.Seed places the 9cm culture dish that is lined with two layers of filter paper, and filter paper is moistening fully with distilled water before seed is put bed.16 d that in illumination box, germinate then, illumination every day 12 h, intensity of illumination 4000lx.Cultivation temperature is set to 11 ℃ of simulation low temperature adverse circumstances.Every processing repeats 50 seeds of every repetition 3 times.Write down the chitting piece number every day, the 16th d calculates germination rate, germination index (GI) and vitality index (VI).Computing formula: GI=∑ (Gt/Dt), VI=S * GI, in the formula: Gt is t days a chitting piece number, and Dt is the germination number of days, and S is full stand long (mm).Germination index and vitality index are the common counters of seed vitality.The germination index value becomes positive correlation with vigor, and the seed vitality index can more comprehensively reflect comprehensive potential ability such as seed germination rate and growth of seedling.
The result shows that after the seed soaking of 5 mg/L calcium chloride solutions was handled, big gold dollar of safflower and MSk326 seed germination rate, germination index and the vitality index under the low temperature adverse circumstance was all high than ck1 and ck2.Wherein the big gold dollar seed of safflower is after 5 mg/L calcium chloride solutions seed soaking is handled, germination rate be untreated seed (ck1) than having improved 24%, improved 5.7% with the ck2 ratio; Seed soaking is handled the back germination index and has been improved 1.72 with the ck1 ratio, has improved 0.4 with the ck2 ratio; Vitality index has improved 14.66 with the ck1 ratio, has improved 3.8 (tables 1) with the ck2 ratio.The big gold dollar seed of safflower after 10 mg/L calcium chloride solutions seed soaking is handled, germination rate be untreated seed (ck1) than having improved 23.3%, improved 5% with the ck2 ratio; Seed soaking is handled the back germination index and has been improved 1.67 with the ck1 ratio, has improved 0.35 with the ck2 ratio; Vitality index has improved 12.35 with the ck1 ratio, has improved 1.49 with the ck2 ratio.For the MSk326 seed, germination rate has improved 20% with the ck1 ratio after the seed soaking of 5 mg/L calcium chloride solutions, has improved 6.3% with the ck2 ratio; Germination index has improved 1.41 with the ck1 ratio, has improved 0.77 with the ck2 ratio; Vitality index has improved 21.68 with the ck1 ratio, has improved 7.29 (tables 2) with the ck2 ratio.MSk326 seed germination rate after the seed soaking of 10 mg/L calcium chloride solutions has improved 20.7% with the ck1 ratio, has improved 7% with the ck2 ratio; Germination index has improved 1.5 with the ck1 ratio, has improved 0.86 with the ck2 ratio; Vitality index has improved 20.6 with the ck1 ratio, has improved 6.21 (tables 2) with the ck2 ratio
The influence to the big gold dollar seed sprouting of tobacco bred safflower under the low temperature stress is handled in the different medicament seed soaking of table 1
| Handle | Germination rate (%) | Germination index (GI) | Vitality index (VI) |
| Ck1 (dry seeds) | 74.0 | 3.18 | 20.21 |
| Ck2 (clear water seed soaking) | 92.3 | 4.50 | 31.07 |
| 5 mg/L calcium chloride | 98.0 | 4.90 | 34.87 |
| 10 mg/L calcium chloride | 97.3 | 4.85 | 32.56 |
The influence to tobacco bred MSk326 seed sprouting under the low temperature stress is handled in the different medicament seed soaking of table 2
| Handle | Germination rate (%) | Germination index (GI) | Vitality index (VI) |
| Ck1 (dry seeds) | 76.0 | 2.52 | 13.81 |
| Ck2 (clear water seed soaking) | 89.7 | 3.16 | 28.20 |
| 5 mg/L calcium chloride | 96.0 | 3.93 | 35.49 |
| 10 mg/L calcium chloride | 96.7 | 4.02 | 34.41 |
Application example 2
Embodiment 1 is soaked seed the back seed as experiment material with embodiment 2 gained, be respectively contrast 1 and 2 (CK1 and CK2) with dry seeds and clear water seed soaking and carry out indoor germination test.Seed places the 9cm culture dish that is lined with two layers of filter paper, filter paper seed put the bed before with 15% PEG solution wetted simulating drought condition.16 d that in illumination box, germinate then, illumination every day 12 h, intensity of illumination 4000lx.Cultivation temperature 30 ℃ of 20 ℃ of (12 h) nights of daytime (12 h).Every processing repeats 50 seeds of every repetition 3 times.Write down the chitting piece number every day, the 16th d calculates germination rate, germination index (GI) and vitality index (VI).
The result shows, seed soaking is handled the back big gold dollar of safflower and germination rate, germination index and the vitality index of MSk326 seed under drought stress and ck1 and ck2 than all being improved.Wherein, the big gold dollar seed of safflower through 5 mg/L calcium chloride seed soaking handle the back germination rate improve the most obvious, with ck1 and ck2 score indescribably height 6.6% and 2%; Germination index and ck1 and ck2 score you can well imagine high 2.45 and 1.29; Vitality index and ck1 and ck2 score you can well imagine high 45.81 and 19.34 (table 3).The big gold dollar seed of safflower after 10 mg/L calcium chloride seed soaking is handled, germination rate and ck1 and ck2 score indescribably height 7% and 2.4%; Germination index and ck1 and ck2 score you can well imagine high 3.59 and 2.43; Vitality index and ck1 and ck2 score you can well imagine high 44.2 and 17.73.
For the MSk326 seed, after 5 mg/L calcium chloride solutions seed soaking is handled, germination rate and ck1 and ck2 score indescribably height 11.3% and 4.6%; Germination index and ck1 and ck2 score you can well imagine high 4.24 and 0.84, vitality index and ck1 and ck2 score indescribably height 75.51 and 11.48 (table 4).The MSk326 seed after 10 mg/L calcium chloride solutions seed soaking is handled, germination rate and ck1 and ck2 score indescribably height 11.6% and 4.9%; Germination index and ck1 and ck2 score you can well imagine high 4.58 and 1.18, vitality index and ck1 and ck2 score indescribably height 80.45 and 16.42.
The influence to the big gold dollar seed sprouting of tobacco bred safflower under the drought stress is handled in the different medicament seed soaking of table 3
| Handle | Germination rate (%) | Germination index (GI) | Vitality index (VI) |
| Ck1 (dry seeds) | 84.7 | 8.73 | 104.67 |
| Ck2 (clear water seed soaking) | 89.3 | 9.89 | 131.14 |
| 5 mg/L calcium chloride | 91.3 | 11.18 | 150.48 |
| 10 mg/L calcium chloride | 91.7 | 12.32 | 148.87 |
The influence to tobacco bred MSk326 seed sprouting under the drought stress is handled in the different medicament seed soaking of table 4
| Handle | Germination rate (%) | Germination index (GI) | Vitality index (VI) |
| Ck1 (dry seeds) | 86.0 | 8.07 | 99.42 |
| Ck2 (clear water seed soaking) | 92.7 | 11.47 | 163.45 |
| 5 mg/L calcium chloride | 97.3 | 12.31 | 174.93 |
| 10 mg/L calcium chloride | 97.6 | 12.65 | 179.87 |
Claims (1)
1. one kind is improved the cold-resistant seed-soaking method with drought-resistant ability of tobacco seed simultaneously; It is characterized in that: at first; Use mass percent concentration to be 0.5-2% liquor natrii hypochloritis disinfection seed 10-20 min; Sterilization back is got seed express developed with distilled water, under 25-30 ℃, is dried to initial moisture content after blotting the surface of the seed moisture; Then, seed is placed the calcium chloride solution of 5-10 mg/L, 20-30 ℃ of dark down seed soaking 12-24 h gets seed express developed with distilled water at last, blots the surface of the seed moisture and is placed on and is dried to initial moisture content under 25-30 ℃, stores for use.
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| CN103374105B (en) * | 2012-04-24 | 2015-06-03 | 中国水利水电科学研究院 | Preparation method of soybean seed soaking drought resistance agent |
| CN104012212A (en) * | 2013-03-03 | 2014-09-03 | 四川农业大学 | Disinfection method suitable for rapid germination of nicotiana benthamiana seeds |
| CN103109687A (en) * | 2013-03-14 | 2013-05-22 | 安徽省农业科学院烟草研究所 | Rapid high-flux sieving method of tobacco drought-resistant idioplasm |
| CN104255114B (en) * | 2014-09-15 | 2016-03-16 | 河南科技大学 | A kind of method for treating seeds simultaneously promoting selfheal seed drought resisting and saline-alkaline tolerance |
| CN104756860B (en) * | 2015-03-24 | 2017-09-01 | 河北省林业科学研究院 | A kind of breeding method for cultivating cold-resistant Hebei elm |
| CN106942283A (en) * | 2017-03-20 | 2017-07-14 | 广西中烟工业有限责任公司 | A kind of tobacco seed sprouts strengthening agent and its preparation, application method |
| CN107047209A (en) * | 2017-05-22 | 2017-08-18 | 贾红瑞 | A kind of method of underwood planting balloonflower root |
| CN107494076A (en) * | 2017-09-28 | 2017-12-22 | 江苏农林职业技术学院 | A kind of improvement, which is handed over, broadcasts the withered and yellow method of roegneria kamoji leaf color |
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| JPS58143881A (en) * | 1982-02-19 | 1983-08-26 | Kurita Water Ind Ltd | Purification of water containing phosphate and organic substance |
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