CN104012212A - Disinfection method suitable for rapid germination of nicotiana benthamiana seeds - Google Patents
Disinfection method suitable for rapid germination of nicotiana benthamiana seeds Download PDFInfo
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- CN104012212A CN104012212A CN201310098865.1A CN201310098865A CN104012212A CN 104012212 A CN104012212 A CN 104012212A CN 201310098865 A CN201310098865 A CN 201310098865A CN 104012212 A CN104012212 A CN 104012212A
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- seed
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- sterile water
- disinfection
- nicotiana benthamiana
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Abstract
The invention relates to a disinfection treatment method of nicotiana benthamiana seeds, and belongs to the fields of biotechnologies and modern agricultural technologies. The method comprises the steps of disinfecting the nicotiana benthamiana seeds for 15 minutes by a sodium hypochlorite solution with the single concentration; and then, cleaning the seeds for five times by sterile water in a super clean bench, soaking the cleaned seeds in sterile water for 12 hours at the temperature of 25 DEG C to enable the seeds to be subjected to hydration, and sowing. The disinfection treatment method has remarkable characteristics, and solves the problem that a relatively small quantity of aseptic seedlings can be obtained due to pollution caused by the nicotiana benthamiana seeds which are not thoroughly sterilized; furthermore, the problem that the germination rate of the nicotiana benthamiana seeds and the seedling growth vigor are influenced due to long disinfection time a conventional method can be solved. Compared with a conventional disinfection method, the disinfection method needs a relatively small quantity of reagents and is simple in operation process; after the hydration acts on the disinfected seeds for a period of time, the germination rate and the germination potential are increased, the seed germination rate reaches more than 98%, the seedlings are healthy and strong in growth at the later period, and various experimental demands can be met.
Description
One, technical field
The present invention relates to a kind of seed disinfection processing method, particularly a kind of this life cigarette seed disinfection processing method, belongs to biotechnology and modern agricultural technology field.
Two, background technology
The advantages such as this life cigarette is the important model plant of research plant virus gene function, genetically modified plants Antiviral Mechanism and vegetable protein transient expression, and it has fast growth, and life cycle is short.In the preparation of this life cigarette aseptic seedling and tissue culture procedures, seed disinfection is the first link of work, and conventional method is first to use 70% ethanol disinfection 30s, and mercuric chloride soaks 15 minutes, then uses sterile water wash.This process method step is loaded down with trivial details, and mercuric chloride is easily residual simultaneously, affects seed germination, and germination vigor is weakened.
Three, summary of the invention
The object of the invention is to overcome the deficiency of existing sterilization method, a kind of this life cigarette seed disinfection processing method is provided, make the seed disinfection of this life cigarette thorough, pollution-free, do not affect again seed germination, growth of seedling stalwartness, fast growth.The present invention is achieved through the following technical solutions: adopt the liquor natrii hypochloritis of single concentration to seed disinfection 15 minutes, can kill various microorganisms, then in superclean bench, use sterile water wash seed 5 times, after cleaning, seed soaks 12 hours at 25 DEG C with sterile water, then sowing, seed germination rate is more than 98%, and germination vigor is strong, simultaneously later stage growth of seedling stalwartness.
The concrete method step of the present invention is as follows:
(1) choose appropriate full this life cigarette seed, in closed container (as centrifuge tube), add fresh preparation 10% liquor natrii hypochloritis's soaking at room temperature 15 minutes, and frequently rock.
(2) in superclean bench with sterile water wash seed 5 times, then add appropriate sterile water at closed container (as centrifuge tube), with submergence seed for well.
(3) closed container together with sterile water wash after seed be placed on 25 DEG C process 12 hours, then in superclean bench planting seed on MS minimal medium.
(4) be placed in 25 DEG C of illumination boxs and cultivate, through cultivating the seedling that obtains sprouting about 15 days.
The present invention has outstanding feature, has solved this life cigarette seed because sterilization does not thoroughly pollute, and obtains the less problem of aseptic seedling; Solve due to long germination rate and the growth of seedling gesture problem that affects this life cigarette seed of conventional method disinfecting time simultaneously.Compared with routine disinfection method, the required reagent of this sterilization method is few, and operating process is simple, and seed after sterilization is through aquation after a while, and germination rate and germination vigor strengthen, and later stage growth of seedling stalwartness, can meet various experiments required.
Claims (2)
1. this life cigarette seed disinfection processing method, it is characterized in that: adopt the liquor natrii hypochloritis of single concentration to seed disinfection 15 minutes, then in superclean bench, use sterile water wash seed 5 times, after cleaning, seed sterile water soaks 12 hours at 25 DEG C, then sows.
2. this raw cigarette seed disinfection processing method according to claim 1, is characterized in that method step is as follows:
(1) choose appropriate full this life cigarette seed and be positioned over (as centrifuge tube) in closed container, add fresh preparation 10% liquor natrii hypochloritis's soaking at room temperature 15 minutes, and frequently rock.
(2) in superclean bench with sterile water wash seed 5 times, then in closed container (as centrifuge tube), add appropriate sterile water, with submergence seed for well.
(3) closed container together with sterile water wash after seed be placed on 25 DEG C process 12 hours, then in superclean bench planting seed on MS minimal medium.
(4) be placed in 25 DEG C of illumination boxs and cultivate, through cultivating the seedling that obtains sprouting about 15 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310098865.1A CN104012212A (en) | 2013-03-03 | 2013-03-03 | Disinfection method suitable for rapid germination of nicotiana benthamiana seeds |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310098865.1A CN104012212A (en) | 2013-03-03 | 2013-03-03 | Disinfection method suitable for rapid germination of nicotiana benthamiana seeds |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104012212A true CN104012212A (en) | 2014-09-03 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310098865.1A Pending CN104012212A (en) | 2013-03-03 | 2013-03-03 | Disinfection method suitable for rapid germination of nicotiana benthamiana seeds |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104012212A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010122372A1 (en) * | 2009-04-21 | 2010-10-28 | Lucia Atehortua Garces | Method for cellular tissue multiplication from jatropha curcas |
| CN102119596A (en) * | 2011-01-29 | 2011-07-13 | 浙江大学 | Seed soaking processing method for simultaneously improving cold and drought resistance of tobacco seed |
-
2013
- 2013-03-03 CN CN201310098865.1A patent/CN104012212A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010122372A1 (en) * | 2009-04-21 | 2010-10-28 | Lucia Atehortua Garces | Method for cellular tissue multiplication from jatropha curcas |
| CN102119596A (en) * | 2011-01-29 | 2011-07-13 | 浙江大学 | Seed soaking processing method for simultaneously improving cold and drought resistance of tobacco seed |
Non-Patent Citations (2)
| Title |
|---|
| 孔凡利 等: "转枯草芽孢杆菌植酸酶基因烟草对不同介质中植酸磷的吸收利用", 《应用生态学报》 * |
| 鲁黎明 等: "不同消毒剂对烟草种子消毒效果及萌发的影响", 《种子》 * |
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Application publication date: 20140903 |