CN106342686A - Method on water culture rooting for tissue culture seedlings of Bougainvillea - Google Patents
Method on water culture rooting for tissue culture seedlings of Bougainvillea Download PDFInfo
- Publication number
- CN106342686A CN106342686A CN201610759199.5A CN201610759199A CN106342686A CN 106342686 A CN106342686 A CN 106342686A CN 201610759199 A CN201610759199 A CN 201610759199A CN 106342686 A CN106342686 A CN 106342686A
- Authority
- CN
- China
- Prior art keywords
- bud
- culture
- water
- stem
- seedling
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Abstract
The invention discloses a method on water culture rooting for tissue culture seedlings of Bougainvillea. The soft stem of Bougainvillea is collected for explant, which is inoculated in the culture medium Ms + 6-ba2.0 mg . L-1 + Naa0.5 mg . L-1 + sucrose 25g . L-1 + agar 5.8g . L-1,to induce adventitious bud. And after seeding is strengthened, it's conducted the water-cultured rooting and then directly conducted the domestication and transplantation. Cycle, which could simplify the experimental procedures,reduce production costs, improve efficiency and could be suitable for large-scale plant nursery.
Description
Technical field
The present invention relates to a kind of plant tissue culture reproduction technique, the method that specially a kind of Bougainvillea spectabilis tissue cultured seedling water planting is taken root.
Background technology
At present, domestic Bougainvillea spectabilis tissue-culturing rapid propagation is mainly broken up with stem section callus induction again and sprouts to set up numerous raw body sooner
System, its growth coefficient, rooting rate is low, and the calluss successive transfer culture time is long, and differentiation is slow, or even is divided into lopsided bud or undifferentiated
The phenomenons such as bud, and outdoor rooting rate is low.
Content of the invention
It is an object of the invention to provide a kind of method that Bougainvillea spectabilis tissue cultured seedling water planting is taken root, can the short tissue culture cycle, simplify real
Test program, reduce production cost, improve work efficiency it is adaptable to plant layout nursery.
For solving above-mentioned technical problem, the technical solution adopted in the present invention is: a kind of Bougainvillea spectabilis tissue cultured seedling water planting is taken root
Method, comprise the following steps:
1) take the Bougainvillea spectabilis full semi-lignified stem with bud of raw no disease and pests harm, bud then, carry out disinfection；
2) the Bougainvillea spectabilis stem with bud disinfecting is carried out with inducing clumping bud, culture medium prescription used is ms+6-ba2.0mg
l-1+naa0.5mg•l-1+ sucrose 25g l-1+ agar 5.8g l-1, ph5.8, condition of culture is 24 ± 1 DEG C of temperature, humidity 80 ±
5%th, illumination 14-16h/d, intensity of illumination 2000-2500lx, obtaining length is 3-5cm Multiple Buds；Multiple Buds are again through the blank training of ms
Foster base carries out strong sprout；
3) seedling exercising is carried out to strong sprout, then cut off blade and the calluss of base portion, retain 3～4 leaves, clear water is cleaned Seedling and sticked
Culture medium, drainage, be then placed in auxin solution soak 1h after, place into be filled with water hole tray interior edema training take root.
Further, described stem with bud contains 2-3 full bud.
Further, during described sterilization, first the stem with bud base portion of collection is soaked 1h with concentration 1mg/lga3, put
In concentration be 0.5% washing powder solution in soak 10min, be subsequently placed in flowing water rinse 1h, superclean bench with concentration is
75% alcohol-pickled 30s, then with 0.1% mercuric chloride sterilization 18min, aseptic water washing 5-6 time, be finally placed on aseptic filter paper
Blot excessive moisture, the stem with bud after being sterilized.
Further, during described strong sprout, first the Multiple Buds of induction are cut into individual plant, on ms blank cultures, every bottle connects 4
Strain, after dark training 10d, placing into condition of culture is 24 ± 1 DEG C of temperature, humidity 80 ± 5%, illumination 14-16h, intensity of illumination 2000-
The culture of 2500lx is indoor, and after culture 15d, plant strain growth is healthy and strong.
Further, the height of seedling 3-5cm of the strong sprout described in step 3), the thick 1.5-2.0cm of stem.
Further, the seedling exercising time described in step 3) is one week, and condition is controlled to 24 ± 1 DEG C of temperature, humidity 85 ± 5%, light
According to 12h/l and intensity of illumination 3000-3500lx.
Further, the formula of described auxin solution is iba1.0mg/l-1+naa0.01mg/l-1.
Further, when described water planting is taken root, the daily carbendazim solution with diluting 800 times sprays blade face once, changes daily
Clear water is once.
The present invention directly utilizes stem section to induce Multiple Buds, reduces callus induction link；Test tube seedling after strong sprout is directly carried out
Outdoor water planting is taken root, time-consuming and production cost.And the test tube seedling outdoor water planting after strong sprout is taken root and is solved tissue culture and take root
A difficult problem for sterile working's easily pollution need to be solved in gnotobasiss to root culture conversion open in natural environment, greatly
Reduce the labor intensity of staff；
The method that the present invention provides, water planting is taken root and is taken root shortening 30-35d than conventional tissue culture.Test tube seedling is taken root by water planting
Decrease the work such as culture medium preparation, indoor aseptic inoculation, culturing room's culture.
Instant invention overcomes the Bougainvillea spectabilis callus from stem segment successive transfer culture time is long, differentiation rate is low, and in vitro rooting induces
Program complexity is loaded down with trivial details, and outdoor rooting rate is low, the shortcomings of high cost.Directly induce Multiple Buds first with stem section, then blank through ms
Culture medium (without phytohormone) strong sprout, finally carries out tissue cultured seedling outdoor water planting and takes root.It directly induces Multiple Buds, propagation system
Number reaches 7.8.Ye Dingying (2011) is taken root in research inside and outside Bougainvillea spectabilis tissue cultured seedling test tube, and outdoor rooting rate is only 43%, this
Bright rooting rate is 93.6%.The present invention not only shortens the tissue culture cycle, simplifies experimental arrangement, and reduces production cost, improves work
Make efficiency it is adaptable to plant layout nursery.
Specific embodiment
With reference to specific embodiment, it is further elucidated with the present invention.
1.1 explant
Bougainvillea spectabilis are taken to give birth to no disease and pests harm, the full semi-lignified stem with bud of bud (containing 2-3 full bud) then.
1.2 explant sterilizations
First by Bougainvillea spectabilis stem with bud base portion concentration 1mg/lga of collection3Soaking 1h → be placed on concentration is 0.5% wash
The alcohol-pickled 30s that 10min → be placed in flowing water rinses 1h → be 75% with concentration on superclean bench is soaked in clothing powder solution,
Sterilized 18min → aseptic water washing 5-6 time → explant is placed on aseptic filter paper and blots excessive moisture with 0.1% mercuric chloride again, obtain
Explant to after sterilization.
Indoor cultivation condition during 1.3 inducing clumping bud: 24 ± 1 DEG C of temperature, humidity 80 ± 5%, illumination 14-16h/d, illumination
Intensity 2000-2500lx.
1.4 inducing clumping bud
Culture medium prescription ms+6-ba2.0mg l-1+naa0.5mg·l-1+ sucrose 25g l-1+ agar 5.8g l-1, ph5.8,
Multiple Buds are produced, after 30d, statistics inducing clumping bud rate is 97.8%, growth coefficient 7.8 after 15d.Every 20d transfers once, continuously later
After switching 3 times, obtaining substantial amounts of length is 3-5cm aseptic seedling.
1.5 strong sprout
When Multiple Buds extend to 3cm and above when, the Multiple Buds of induction are cut into individual plant, access culture medium ms blank (without
Any hormone) on, every bottle connects 4 plants, and after dark training 10d, placing into condition of culture is 24 ± 1 DEG C of temperature, humidity 80 ± 5%, illumination
14-16h, the culture interior of intensity of illumination 2000-2500lx, after culture 15d, plant strain growth is healthy and strong.
1.6 water plantings are taken root
Choose height of seedling 3-5cm, the test tube seedling of the thick 1.5-2.0cm of stem to carry out water planting and take root, warmhouse booth temperature control is 24 ± 1
DEG C %, humidity 85 ± 5%, illumination 12h/l, intensity of illumination 3000-3500lx.After seedling exercising one week, open bottle cap and take out test tube seedling, cut
Remove blade and the calluss of base portion, retain 3～4 leaves, clear water cleans the culture medium that test tube seedling Seedling sticks, be placed on filter on newspaper
Water, then after being respectively put into and soaking 1h in the different auxin solution preparing, the hole tray interior edema training being then placed in being filled with water is taken root, daily
800 times of carbendazim solutions spray blade face water once, change clear water daily once, keep the cleaning of water.
Using two factor three horizontal experimental design, Bougainvillea spectabilis test tube seedling is carried out with water planting to take root research, iba (0.5mg/l-1、
1.0mg/l-1、1.5mg/l-1、0mg/l-1), naa(0mg/l-1、0.5mg/l-1、1.0mg/l-1、1.5mg/l-1) at hormon
Reason, often processes 3 repetitions, often repeats 10 plants of test tube seedlings.Through 3-5d, substantially root all starts to occur small white spots, forms root
Source base, 7-10d, root base portion bears one-level root, and elongation growth, and through 30 natural law according to statistics, different naa concentration are averagely taken root
Rate is 83.2%, 84.3%, 86.7%, and the average rooting rate of different iba concentration is 85.9%, 86.3%, 87.2%, and hormon iba
Combining down average rooting rate with naa is 85.3%, 84.6%, 85.1%, 86.3%, 85.9%,
90.1%, 93.6%.91.2%, 90.8%, average primary root take root bar number be respectively 2.2,2.5,2.6,2.15,
Article 2.3,2.6,2.76,2.3,2.5,2.1,2.75,2.81,3.4,3.25,2.9；Most preferably raw
Root hormone combinations iba1.0mg/l-1+naa0.01mg/l-1, average rooting rate is 92.4%, and average primary takes root bar number for 3.4
Bar, wherein the comparison average rooting rate of ck are 55%, and average primary takes root bar number for 2.1.Although indoor rooting rate also can reach
92.53%, but from simplifying program, reducing workload and reducing the consideration of the composite factors such as production cost, outdoor water planting has very excellent greatly
Gesture.
1.7 transplanting
Gently take out from aqueous solution, the Bougainvillea spectabilis plant after taking root, do not injure root, remove the luxuriant blade of base portion growing way, on
To two kinds of substrate, it is ensured that its breathability, one kind is perlite and peat 1:1 to a kind of loam of common loose to basin, every basin one
Strain, each 10 basins, in triplicate, pour within first day permeable, see later dry see wet, blade face water can be spread daily and ensure humidity, transplant three weeks
Survival rate substantially constant (whether being launched according to young leaves, whether plant growing way normally judges whether survives) afterwards, its growth week
Phase shortens 25d than tissue culture.The larger environment of Bougainvillea spectabilis workable moisture it is ensured that daily air humidity, accomplish to see dry see wet.
The Bougainvillea spectabilis tissue cultured seedling water planting that the present invention is disclosed is taken root fast breeding technique, gives birth to stem section then as explant with Bougainvillea spectabilis
Body, is inoculated in culture medium ms+ 6-ba2.0 mg l-1+naa0.5 mg·l-1+ sucrose 25g l-1+ agar 5.8g l-1Formula
On, induce Multiple Buds；Take root carrying out water planting after propagation Seedling strong sprout, then transplant seedling exercising again, be conducive to shortening the tissue culture cycle, fall
Low production cost is it is adaptable to batch production scale seedling raising.
The above embodiments are only the preferred technical solution of the present invention, and are not construed as the restriction for the present invention, this
The technical scheme that bright protection domain should be recorded with claim, technical characteristic in the technical scheme recorded including claim
Equivalents are protection domain.I.e. equivalent within this range is improved, also within protection scope of the present invention.
Claims (8)
1. a kind of method that Bougainvillea spectabilis tissue cultured seedling water planting is taken root is it is characterised in that comprise the following steps: 1) takes Bougainvillea spectabilis then
The full semi-lignified stem with bud of raw no disease and pests harm, bud, carries out disinfection；2) clump is carried out to the Bougainvillea spectabilis stem with bud disinfecting
Sprout induction, culture medium prescription used is ms+6-ba2.0mg l-1+naa0.5mg•l-1+ sucrose 25g l-1+ agar 5.8g
l-1, ph5.8, condition of culture is 24 ± 1 DEG C of temperature, humidity 80 ± 5%, illumination 14-16h/d, intensity of illumination 2000-2500lx, obtains
It is 3-5cm Multiple Buds to length；Multiple Buds carry out strong sprout through ms blank cultures again；3) seedling exercising is carried out to strong sprout, then cut off
The blade of base portion and calluss, retain 3～4 leaves, and clear water cleans the culture medium that Seedling sticks, drainage, is then placed in auxin
After soaking 1h in solution, place into the hole tray interior edema training being filled with water and take root.
2. method according to claim 1 it is characterised in that: described stem with bud contains 2-3 full bud.
3. method according to claim 1 it is characterised in that: during described sterilization, first by collection stem with bud base portion use
Concentration 1mg/lga3 soaks 1h, is placed on immersion 10min in the washing powder solution that concentration is 0.5%, is subsequently placed in flowing water and rinses
1h, the alcohol-pickled 30s being 75% with concentration on superclean bench, then with 0.1wt% mercuric chloride sterilize 18min, aseptic water washing
5-6 time, finally it is placed on aseptic filter paper and blots excessive moisture, the stem with bud after being sterilized.
4. method according to claim 1 it is characterised in that: during described strong sprout, first the Multiple Buds of induction are cut into individual plant,
On ms blank cultures, every bottle connects 4 plants, and after dark training 10d, placing into condition of culture is 24 ± 1 DEG C of temperature, humidity 80 ± 5%, light
Culture according to 14-16h, intensity of illumination 2000-2500lx is indoor, and after culture 15d, plant strain growth is healthy and strong.
5. method according to claim 1 it is characterised in that: the thick 1.5- of the height of seedling 3-5cm of the strong sprout described in step 3), stem
2.0cm.
6. method according to claim 1 it is characterised in that: the seedling exercising time described in step 3) be one week, condition is controlled to
24 ± 1 DEG C of temperature, humidity 85 ± 5%, illumination 12h/l and intensity of illumination 3000-3500lx.
7. method according to claim 1 it is characterised in that: the formula of described auxin solution be iba1.0mg/l-1+
naa0.01mg/l-1.
8. method according to claim 1 it is characterised in that: daily many bacterium with 800 times of dilution when described water planting is taken root
Water once, is changed once daily in clever spray solution blade face.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610759199.5A CN106342686B (en) | 2016-08-30 | 2016-08-30 | A kind of method that Bougainvillea spectabilis tissue-cultured seedling water planting is taken root |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610759199.5A CN106342686B (en) | 2016-08-30 | 2016-08-30 | A kind of method that Bougainvillea spectabilis tissue-cultured seedling water planting is taken root |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106342686A true CN106342686A (en) | 2017-01-25 |
| CN106342686B CN106342686B (en) | 2019-01-01 |
Family
ID=57857343
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610759199.5A Active CN106342686B (en) | 2016-08-30 | 2016-08-30 | A kind of method that Bougainvillea spectabilis tissue-cultured seedling water planting is taken root |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106342686B (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109258467A (en) * | 2018-10-16 | 2019-01-25 | 广西壮族自治区林业科学研究院 | A method of preventing bougainvillea tissue-cultured seedling yellow |
| CN111226770A (en) * | 2020-03-07 | 2020-06-05 | 广州甘蔗糖业研究所湛江甘蔗研究中心 | Liquid seedling hardening method for improving transplanting survival rate of tissue culture seedlings of cinnamomum kanehirae |
| CN111903487A (en) * | 2019-07-29 | 2020-11-10 | 海南省农业科学院热带园艺研究所 | Efficient transplanting method for bougainvillea spectabilis tissue culture seedlings and preparation method of matrix and nutrient solution |
| CN112673957A (en) * | 2020-12-22 | 2021-04-20 | 中国热带农业科学院热带作物品种资源研究所 | Rapid breeding method of bougainvillea spectabilis tissue culture plants |
| CN115428731A (en) * | 2022-08-11 | 2022-12-06 | 厦门路桥百城建设投资有限公司 | Method for rapidly inducing roots through tide-type in-vitro cutting of bougainvillea spectabilis and application of method |
| CN116019013A (en) * | 2023-01-31 | 2023-04-28 | 厦门市园林植物园 | Fast breeding method of bougainvillea spectabilis |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105409509A (en) * | 2015-11-12 | 2016-03-23 | 黄福忠 | Rapid propagation method of Bougainvillea spectabilis Willd |
-
2016
- 2016-08-30 CN CN201610759199.5A patent/CN106342686B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105409509A (en) * | 2015-11-12 | 2016-03-23 | 黄福忠 | Rapid propagation method of Bougainvillea spectabilis Willd |
Non-Patent Citations (2)
| Title |
|---|
| SYED TARIQ SHAH ET.AL.,: "MASS PROPAGATION OF BOUGAINVILLEA SPECTABILIS THROUGH SHOOT TIP CULTURE", 《PAK. J. BOT.,》 * |
| 叶顶英: "三角梅组培苗试管内外生根研究", 《北方园艺》 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109258467A (en) * | 2018-10-16 | 2019-01-25 | 广西壮族自治区林业科学研究院 | A method of preventing bougainvillea tissue-cultured seedling yellow |
| CN111903487A (en) * | 2019-07-29 | 2020-11-10 | 海南省农业科学院热带园艺研究所 | Efficient transplanting method for bougainvillea spectabilis tissue culture seedlings and preparation method of matrix and nutrient solution |
| CN111903487B (en) * | 2019-07-29 | 2022-01-18 | 海南省农业科学院热带园艺研究所 | Efficient transplanting method for bougainvillea spectabilis tissue culture seedlings and preparation method of matrix and nutrient solution |
| CN111226770A (en) * | 2020-03-07 | 2020-06-05 | 广州甘蔗糖业研究所湛江甘蔗研究中心 | Liquid seedling hardening method for improving transplanting survival rate of tissue culture seedlings of cinnamomum kanehirae |
| CN112673957A (en) * | 2020-12-22 | 2021-04-20 | 中国热带农业科学院热带作物品种资源研究所 | Rapid breeding method of bougainvillea spectabilis tissue culture plants |
| CN115428731A (en) * | 2022-08-11 | 2022-12-06 | 厦门路桥百城建设投资有限公司 | Method for rapidly inducing roots through tide-type in-vitro cutting of bougainvillea spectabilis and application of method |
| CN115428731B (en) * | 2022-08-11 | 2024-04-19 | 厦门路桥百城建设投资有限公司 | Triangle Mei Chaoxi type in-vitro cutting rapid root induction method and application thereof |
| CN116019013A (en) * | 2023-01-31 | 2023-04-28 | 厦门市园林植物园 | Fast breeding method of bougainvillea spectabilis |
| CN116019013B (en) * | 2023-01-31 | 2024-03-19 | 厦门市园林植物园 | Fast breeding method of bougainvillea spectabilis |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106342686B (en) | 2019-01-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106342686A (en) | Method on water culture rooting for tissue culture seedlings of Bougainvillea | |
| CN111616052A (en) | Rapid propagation and sugar-free rooting culture method and application of apple rootstock catalpa bungei | |
| CN104255491B (en) | A kind of gold leaf tortoise plastron Chinese ilex tissue culture propagation method | |
| CN106665357A (en) | Method for establishing lycoris regeneration system | |
| CN107667860A (en) | A kind of tissue culture propagation of Alpinia japonica | |
| CN106857254A (en) | The efficient tissue culture and rapid propagation method of apple rootstock green grass or young crops No. 1 elite stand of anvil | |
| CN108419675A (en) | A kind of tissue culture method of violet passion fruit top tip | |
| CN106922536A (en) | A kind of method that fast energy-saving cultivates bletilla seedling | |
| CN103651115A (en) | Rapid propagation method of rhododendron azalea tissue culture | |
| CN108077071A (en) | Vitex agnus-castus tissue cultures culture medium and rapid propagation method | |
| CN106106178A (en) | A kind of method for tissue culture of confection Rhizoma Iridis Tectori | |
| CN106550875B (en) | For the MS culture mediums of rouge radish tissue cultures, the quick breeding method for tissue culture of adventitious shoots culture base and rouge radish | |
| CN105349432A (en) | Puccinia polysora underw single-spore propagation method | |
| CN110402818B (en) | Tissue culture and rapid propagation seedling raising method for mature embryos of high-quality Chinese chestnuts | |
| CN105284622B (en) | A kind of method that quick acquisition Rhizoma Iridis Tectori hybridizes superior clone | |
| CN112167058A (en) | Rapid propagation method for improved plant tissue culture | |
| CN115606503B (en) | Tissue culture method of aster | |
| CN107371880A (en) | A kind of apple rootstock tissue culturing fast seedling-cultivating method | |
| CN103843664A (en) | Lycium exsertum tissue culture and rapid propagation method | |
| CN116058285A (en) | Tissue culture method of dendrobium huoshanense | |
| CN106359107A (en) | Tissue culture method of wild lilium | |
| CN103430822B (en) | Aquaculture seed reproduction method for micro seed tubers of konjac | |
| CN110100740A (en) | A kind of tissue culture and rapid propagation method of two discriminations Platycerium bifurcatum | |
| CN102232359A (en) | In-vitro rapid propagation method of double-petal Jasminum sambac | |
| CN109329059A (en) | A kind of method of straw short-tube lycoris tissue cultures |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20181114 Address after: No. 1 yuyuyutan South Road, Beijing, Beijing Applicant after: China Yangtze River Three Gorges Group Co., Ltd. Address before: 443001 No. 80 Dongshan Avenue, Yichang City, Hubei Province Applicant before: Three Gorges Ecology Garden Co., Ltd. |
|
| TA01 | Transfer of patent application right | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |