CN103918378A - Seed processing method for improving the capability of soybean for resisting saline-alkali stress - Google Patents
Seed processing method for improving the capability of soybean for resisting saline-alkali stress Download PDFInfo
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- CN103918378A CN103918378A CN201410142570.4A CN201410142570A CN103918378A CN 103918378 A CN103918378 A CN 103918378A CN 201410142570 A CN201410142570 A CN 201410142570A CN 103918378 A CN103918378 A CN 103918378A
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Abstract
The invention relates to a seed processing method for improving the capability of soybean for resisting saline-alkali stress. The method specifically comprises the following steps of: a, preparing a treating fluid; b, cleaning soybean seeds, selecting the full soybean seeds, and removing soil on the selected seeds through washing by distilled water, and then draining; c, soaking the seeds; d, drying the seeds; and e, coating the seeds generally, and then sealing the soaked, cleaned and dried seeds in bags after coating generally for later seeding. After the soybean seeds are processed by the method provided by the invention, the soybean seeds can germinate in advance under saline-alkali stress conditions, and the germination rate, germination potential and seed vigor of the seeds can be enhanced; furthermore, the seedling height is increased, the seedling resistance is enhanced, and leaves are dark green due to the increase of pigment content.
Description
Technical field
The present invention relates to crops process field, relate in particular to a kind of method for treating seeds that improves soybean and resist Saline Alkali Stress ability.
Background technology
There are nearly 66.7 ten thousand hm of saline-alkali soil the saline and alkaline arid area of Western Heilongjiang Province and part farm, reclamation area
2, the song-Nen plain low lying areas that mainly distribute, Fujin, innerland, the Three River Plain, friendship one are with also has slight saline and alkaline soil to distribute.These saline and alkaline arid area soybean planting areas only account for 10% left and right of arable area, and soybean per unit area yield is low, and gross yield is little.How to improve the soybean per unit area yield of this area, expand cultivated area, increase the soybean total volume of commodities, have great significance for the economic development that promotes reclamation area and Oils and fats enterprise.
Current many crops are all to alleviate the soil salinization, the impact of secondary salinization on agricultural production by cultivating salt tolerant kind.But cultivate salt tolerant soybean varieties owing to lacking available salt tolerant breeding material, adopt conventional breeding technique not yet can cultivate so far gratifying salt tolerant soybean varieties, and process is slow.
Chemical agent seed soaking, from the angle of physiological metabolism, can be induced some Physiology and biochemistry processes of seed germination incipient stage, as the activation of the degraded of breaking dormancy, inhibitor, imbitition, enzyme etc., improves the vigor of seed; Once condition is applicable to, seed just can absorb water rapidly, recovers the metabolic process of seed.Moisture absorption-Hui Gan is a kind of pretreated method before seed is broadcast and before storage, is that seed first absorbs water and dehydrates the process of getting back to original weight again.Moisture absorption Hui Gan changes the physiology of Interior Seed and biochemical indicator, thereby improves to a certain extent the resistance in germinating capacity, vitality and the seed regrowth growth course of seed.
In view of above-mentioned defect, creator of the present invention has obtained this creation finally through long research and practice.
Summary of the invention
The object of the present invention is to provide a kind of method for treating seeds that improves soybean and resist Saline Alkali Stress ability, in order to overcome above-mentioned technological deficiency.
For achieving the above object, the invention provides a kind of method for treating seeds that improves soybean and resist Saline Alkali Stress ability,
This detailed process is:
Step a, preparation treatment fluid;
Step b, cleans soya seeds, is filtered dry after the full soya seeds distilled water of picking out is washed away to earth;
Step c, soaks seed;
Steps d, seed returns dry treatment;
Step e, conventional dressing, carries out conventional dressing operation by the seed that completes immersion, cleaning Hui Gan and processes back pkt. envelope for broadcasting.
Further, in above-mentioned steps a, described treatment fluid comprises white vitriol 0.25g/L, calcium chloride dihydrate 0.0525mol/L, betain 0.5g/L and gibberellin 0.03g/L.
Further, in above-mentioned steps a, preparation when treatment fluid, is first dissolved gibberellin with minute quantity ethanol, dissolves betain, calcium chloride dihydrate and gibberellin with distilled water; After adding white vitriol to dissolve before use, mix for subsequent use.
Further, in above-mentioned steps c, clean soya seeds is put in the treatment fluid of 4-5 times of grain weight, is put in 25 DEG C of insulating boxs and soaks 2h, then outwell treatment fluid, with distilled water cleaning 3 times.
Further, in above-mentioned steps d, the seed after soaking and washing is placed in to shady and cool ventilation place dry, or is put in 30 DEG C of baking ovens and dries, while only increasing 5%-6% to soya seeds weight compared with dry seeds weight, stop, for subsequent use.
Compared with prior art beneficial effect of the present invention is, after the inventive method processed soybeans seed, can make soya seeds sprout in advance under Saline Alkali Stress condition, can improve germination rate, germination vigor and the seed vitality of seed; And increase Seedling Height, make Resistance of Seedling strengthen, blade because of pigment content increase dark green.
Embodiment
Below, the present invention above-mentioned technical characterictic and advantage with other are described in more detail.
The present invention is a kind of improves soybean and resists the method for treating seeds of Saline Alkali Stress ability, that clean soya seeds is put in the treatment fluid of 4-5 times of grain weight, soak 2h in 25 DEG C, then outwell treatment fluid, with after distilled water cleaning 3 times, air-dry or in 30 DEG C of baking ovens Hui Gan, while only increasing 5%-6% to soya seeds weight, directly carry out after seed germination experiment or conventional dressing operation are processed waiting to broadcast.Through said method soya seeds after treatment, can in 7d, keep corresponding vigor.
The concrete treatment step of this method, comprises following process:
Step a, preparation treatment fluid:
Treatment fluid being constructed as follows after experiment of single factor and orthogonal optimization: comprise white vitriol 0.25g/L, calcium chloride dihydrate 0.0525mol/L, betain 0.5g/L and gibberellin 0.03g/L.
When preparation, first with minute quantity ethanol, gibberellin is dissolved, dissolve betain, calcium chloride dihydrate and gibberellin with distilled water; After adding white vitriol to dissolve before use, mix for subsequent use;
Step b, cleans soya seeds:
After being washed away to earth, the full soya seeds distilled water of picking out is filtered dry;
Step c, soaks seed:
Clean soya seeds is put in the treatment fluid of 4-5 times of grain weight, is put in 25 DEG C of insulating boxs and soaks 2h, then outwell treatment fluid, with distilled water cleaning 3 times;
Steps d, seed returns dry treatment:
Seed after soaking and washing is placed in to shady and cool ventilation place dry, or is put in 30 DEG C of baking ovens and dries, while only increasing 5%-6% to soya seeds weight compared with dry seeds weight, stop, for subsequent use;
Step e, conventional dressing:
The seed that completes immersion, cleaning Hui Gan is carried out to conventional dressing operation and process back pkt. envelope for broadcasting.
Describe below by each experiment:
Embodiment mono-:
With the treatment fluid of 4-5 times of grain weight, treatment fluid is containing white vitriol 0.25g/L, calcium chloride dihydrate 0.0525mol/L, betain 0.5g/L and gibberellin 0.03g/L, soak the anti-line of kind No. 10 soya seeds, outwell treatment fluid soak 2h in 25 DEG C of insulating boxs after, clean 3 times with distilled water; Seed after soaking and washing is placed in to shady and cool ventilation place dry, or is put in 30 DEG C of baking ovens and dries, while only increasing 5%-6% to soya seeds weight compared with dry seeds weight, stop.After seed after immersion Hui Gan is placed on to clean sterilizing, be encased inside in the culture dish of filter paper, 50, every ware, adds after 50mmol/L Saline Alkali Stress liquid 20mL, and this salt acid stress fluid component is NaHCO3: Na
2cO
3=5: 1, in 25 DEG C of insulating boxs, cultivate, record seed sprouting number every day, after 3d, respectively get after 10 sprouted broad beans are weighed and calculate seed vitality.It is to process seed with the distilled water of 4-5 times of grain weight that water soaking returns dry treatment, soak and return dry condition, and seed germination experiment is steeped back dry experiment with processing immersion.Untreated is directly by dry seeds, cleans its surperficial earth with clean wet towel, sprouts experiment, cultivates and Saline Alkali Stress condition steep back and done and water soaking returns dry treatment group with processing immersion.Seed vitality index is respectively 82.62,275.80 and 321.44 in untreated, water soaking Hui Gan and treatment fluid soak back dry group.
Embodiment bis-:
Selecting No. 8 soya seeds of the anti-line of kind is material, and untreated, water soaking Hui Gan and treatment fluid soak back the seed processing technology of dry each group and sprout condition of culture with embodiment mono-.
In the present embodiment, seed vitality index is respectively 92.82,159.63 and 195.48 in untreated, water soaking Hui Gan and treatment fluid soak back dry group.
Embodiment tri-:
Selecting No. 4 soya seeds of the anti-line of kind is material, and untreated, water soaking Hui Gan and treatment fluid soak back the seed processing technology of dry each group and sprout condition of culture with embodiment mono-.
In this example, seed vitality index is respectively 77.28,109.14 and 229.25 in untreated, water soaking Hui Gan and treatment fluid soak back dry group.
Concrete case study on implementation in seedling stage experimentation.
Embodiment tetra-:
With the treatment fluid of 4-5 times of grain weight, this treatment fluid is containing white vitriol 0.25g/L, calcium chloride dihydrate 0.0525mol/L, betain 0.5g/L and gibberellin 0.03g/L, soak No. 10 soya seeds of the anti-line of kind, soak 2h in 25 DEG C of insulating boxs after, outwell treatment fluid, with distilled water cleaning 3 times; Seed after soaking and washing is placed in to shady and cool ventilation place dry, or is put in 30 DEG C of baking ovens and dries, while only increasing 5%-6% to soya seeds weight compared with dry seeds weight, stop.Water soaking Hui Gan and untreated fish group seed treatment are with bud phase embodiment.3 kinds are completed after seed treatment, carry out after conventional dressingization is processed sowing.Adopt sand base cultivation, after planting first water Hoagland nutrient solution, use the 10mmol/L Saline Alkali Stress liquid of Hoagland compounding instead and thoroughly fill with after cotyledon is unearthed, the component of this Saline Alkali Stress liquid is NaHCO3: Na
2cO
3=5: 1.After cotyledon launches completely, coerce liquid concentration and be increased to 20mmol/L, use Hoagland compounding, within every two days, thoroughly fill with once.The each index of sampling and measuring after 3 weeks.
From the various embodiments described above, obtain experimental data.
Refer to described in table 1, it is the impacts of various processing on soybean germination rate, germination vigor and seed vitality.
The impact of the various processing of table 1 on soybean germination rate, germination vigor and seed vitality
It can be seen from the table, the present invention improves soybean and resists the method for treating seeds of Saline Alkali Stress ability, can make soya seeds sprout in advance under Saline Alkali Stress condition, can improve germination rate, germination vigor and the seed vitality of seed.
Refer to described in table 2, it is the impacts of various processing on soybean seedling index.
The impact of the various processing of table 2 on soybean seedling index
It can be seen from the table, use the method process seed, can increase Seedling Height, make Resistance of Seedling strengthen, blade because of pigment content increase dark green.
Soya seeds is after the method is processed, because seed recovers original appearance state substantially through returning dry process.Therefore, applicable to field machineryization sowing after seed pelleting.
Refer to described in table 3, it is the diurnal variation of SOD activity in soya seeds after processing;
Table 3 is processed the diurnal variation of SOD activity in rear soya seeds
Note: in table, SOD activity is decided to be 100, the relative activity when SOD activity of later every day is relative 0d when 0d.
Seed, after the method is processed, by measuring the active variation of superoxide dismutase (SOD), determines that the seed of processing can keep corresponding active in 7d.
The foregoing is only preferred embodiment of the present invention, is only illustrative for invention, and nonrestrictive.Those skilled in the art is understood, and in the spirit and scope that limit, can carry out many changes to it in invention claim, amendment, and even equivalence, but all will fall within the scope of protection of the present invention.
Claims (5)
1. improve the method for treating seeds that soybean is resisted Saline Alkali Stress ability, it is characterized in that, this detailed process is:
Step a, preparation treatment fluid;
Step b, cleans soya seeds, is filtered dry after the full soya seeds distilled water of picking out is washed away to earth;
Step c, soaks seed;
Steps d, seed returns dry treatment;
Step e, conventional dressing, carries out conventional dressing operation by the seed that completes immersion, cleaning Hui Gan and processes back pkt. envelope for broadcasting.
2. raising soybean according to claim 1 is resisted the method for treating seeds of Saline Alkali Stress ability, it is characterized in that, in above-mentioned steps a, described treatment fluid comprises white vitriol 0.25g/L, calcium chloride dihydrate 0.0525mol/L, betain 0.5g/L and gibberellin 0.03g/L.
3. raising soybean according to claim 2 is resisted the method for treating seeds of Saline Alkali Stress ability, it is characterized in that, in above-mentioned steps a, when preparation treatment fluid, first with minute quantity ethanol, gibberellin is dissolved, dissolve betain, calcium chloride dihydrate and gibberellin with distilled water; After adding white vitriol to dissolve before use, mix for subsequent use.
4. raising soybean according to claim 1 and 2 is resisted the method for treating seeds of Saline Alkali Stress ability, it is characterized in that, in above-mentioned steps c, clean soya seeds is put in the treatment fluid of 4-5 times of grain weight, be put in 25 DEG C of insulating boxs and soak 2h, then outwell treatment fluid, with distilled water cleaning 3 times.
5. raising soybean according to claim 1 and 2 is resisted the method for treating seeds of Saline Alkali Stress ability, it is characterized in that, in above-mentioned steps d, seed after soaking and washing is placed in to shady and cool ventilation place dry, or be put in 30 DEG C of baking ovens and dry, while only increasing 5%-6% to soya seeds weight compared with dry seeds weight, stop, for subsequent use.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106717289A (en) * | 2017-03-01 | 2017-05-31 | 民勤县全盛永泰农业有限公司 | A kind of method for improving arid salt-soda soil edible sunflower percentage of seedgermination |
| CN107409532A (en) * | 2017-07-21 | 2017-12-01 | 广西柳城县成霖农业科技有限公司 | A kind of seed-soaking method for improving Soybean Resistance Saline Alkali Stress ability |
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| JPS63270612A (en) * | 1986-12-26 | 1988-11-08 | Ishihara Hiryo Kogyo Kk | Oxygen feeder for agricultural purposes |
| CN102144442A (en) * | 2010-11-29 | 2011-08-10 | 中国科学院新疆生态与地理研究所 | Treatment method for improving salt resistance of wheat seeds |
| CN103109620A (en) * | 2012-11-16 | 2013-05-22 | 高润宝 | Osmoregulation initiation method of corn seeds under salt stress |
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2014
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63270612A (en) * | 1986-12-26 | 1988-11-08 | Ishihara Hiryo Kogyo Kk | Oxygen feeder for agricultural purposes |
| CN102144442A (en) * | 2010-11-29 | 2011-08-10 | 中国科学院新疆生态与地理研究所 | Treatment method for improving salt resistance of wheat seeds |
| CN103109620A (en) * | 2012-11-16 | 2013-05-22 | 高润宝 | Osmoregulation initiation method of corn seeds under salt stress |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106717289A (en) * | 2017-03-01 | 2017-05-31 | 民勤县全盛永泰农业有限公司 | A kind of method for improving arid salt-soda soil edible sunflower percentage of seedgermination |
| CN107409532A (en) * | 2017-07-21 | 2017-12-01 | 广西柳城县成霖农业科技有限公司 | A kind of seed-soaking method for improving Soybean Resistance Saline Alkali Stress ability |
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