CN102115490A - Method for producing blood pressure depressed peptide by using egg white as raw material - Google Patents
Method for producing blood pressure depressed peptide by using egg white as raw material Download PDFInfo
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- CN102115490A CN102115490A CN 201010556411 CN201010556411A CN102115490A CN 102115490 A CN102115490 A CN 102115490A CN 201010556411 CN201010556411 CN 201010556411 CN 201010556411 A CN201010556411 A CN 201010556411A CN 102115490 A CN102115490 A CN 102115490A
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- gallus domesticus
- ovum gallus
- domesticus album
- ultrafiltration
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Abstract
The invention relates to a method for producing blood pressure depressed peptide by using egg white as a raw material, which comprises the following steps of: mixing the egg white with vinegar in a total acidity range of 5-15g/100ml, uniformly stirring, standing at the temperature of between 30 and 40DEG C for 6 to 10 hours, performing ultrafiltration on feed liquor for 10 to 30 minutes by adopting an ultrafiltration film of between 10 and 1kDa, and respectively making filtrate obtained through ultrafiltration pass through H+ cation exchange resin and OH- anion exchange resin. The blood pressure depressed peptide prepared from the egg white serving as the raw material by the method has the angiotensin converting enzyme (ACE) inhibition ratio of 89.2 percent and the hydrolyzing degree of 24.9 percent.
Description
Technical field
The present invention relates to a kind of method of production step-down peptide, particularly relating to a kind of is the method for raw material production step-down peptide with the Ovum Gallus domesticus album.
Background technology
Hypertension is a kind of common cardiovascular disorder, its sickness rate height, often with the functional or organic change of heart, blood vessel, lung and kidney and other organs, be to cause various complication such as the heart, brain, kidney and eye blood vessel and cause apoplexy, promote the significant risk factor of atherosclerosis, coronary heart disease.The hypertension commitment does not generally have any malaise symptoms, and until the outbreak of clinical picture one heart trouble, rupture of blood vessel in brain take place, so hypertension be otherwise known as " noiseless killer ".. the hypertension incidence rate reaches more than 20% in developed country, and the average attack rate of countries in the world is 10%-20%, and the crowd distributes extensively, comprises that the elderly, middle-aged people and children whole world every year reach 1,200 ten thousand because of the number of hypertension death.China had once carried out nationwide hypertension census three times, and the sickness rate of nineteen fifty-nine is that 5.11%, 1979～1980 annual morbidities are that 7.73%, 1990～1991 annual morbidities are 11.26%.At present, the existing hyperpietic of China has surpassed 1.2 hundred million populations.Therefore, treatment and preventing hypertension disease are current social crucial problems.
Hypertension generally has two types: essential hypertension and insecondary hypertension, the former mainly be since around the arteriole external caliber diminish or blood viscosity increases, cause Peripheral resistance too high due to; And Q volume of blood and kinemicly increase, the filling degree of the stiff degree of blood vessel and blood vessel all influences the variation of blood pressure; Autonomous nervous system also is an important factor to the regulating effect of blood pressure simultaneously.Functional or organic disease takes place in any link that influences blood pressure in addition, all can cause hypertension.These factors mainly by sodium salt in heredity, obesity, calcium deficiency, the meals too much, factors such as smoking, nervous or other environmental stimuluses cause that course of disease development is slower.Insecondary hypertension also is symptomatic hypertension, is elevation of blood pressure concurrent in some lysis, and it is unusual etc. that its origin cause of formation mainly contains incretopathy, kidney disease, heart function.The common disease that wherein causes renal hypertension has: parenchymal lesion of the kidney, renal vascular diseases, urinary tract infraction property disease and perinephric inflammation, abscess, tumour, wound, hemorrhage etc.Cause that hypertensive medicine has: female oral contraceptives, prolonged application brain adrenocortical hormone etc.
(angiotensin converting enzyme. ACE) is a kind of zinciferous pepx to angiotensin-converting enzyme, can be activated and have the substrate specificity of broad by chlorion.ACE extensively is distributed in the mammiferous tissue.Renin-angiotensin system (renin-angiotensin system, RAS) and kallikrein one kinin system (kallikrein-kinin system, KKS) in, ACE plays important regulatory role to body blood pressure and cardiovascular function.The activity that suppresses ACE is considered to treat hypertensive a kind of important and effective means.At present, efficient, special in a large number ACE inhibitor is being brought into play important effect in treatment essential hypertension and congestive heart failure.
Though a little less than the ACE inhibitor effect of ace inhibitory peptide than synthetic, it has unique advantage in the food: only the hyperpietic is played the effect of step-down, the normotensive is not had hypotensive effect, therefore can not produce step-down over-drastic problem; These peptides are that the enzyme with food grade makes under mild conditions, and its security is high, has no side effect; It has effects such as anti-oxidant, digestion promoting, hypoglycemic, anti-platelet aggregation, enhancing body immunity simultaneously toward contact except the function that step-down is arranged; Have higher thermostability, advantage such as water-soluble, thereby can be used as functional factor again and add to and go in beverage, the food, market outlook are fabulous.Along with developing rapidly of protein engineering and enzyme engineering technology, also will have a large amount of, highly active antihypertensive activity peptide and be found.The utilization biotechnology is used for foodstuff additive, protective foods, medicine etc. to these peptides, by multiple channel the hyperpietic is obtained medical treatment.
At present, existing research report is isolated the active polypeptide with antihypertensive function from multiple animal-plant material and tankage.As viper venom, cheese, sardines, Semen Maydis grit, soybean meal, fermenting bean milk, collagen protein etc.Now the extraction process that adopts mainly contains 3 kinds: enzymolysis process, directly from leavened food the separation and Extraction method and from autolyzate extraction method.
Summary of the invention
The purpose of this invention is to provide a kind of is the method for raw material production step-down peptide with the Ovum Gallus domesticus album, and in this way Zhi Bei Ovum Gallus domesticus album is that the ACE inhibiting rate of raw material production step-down peptide is 89.2%, and degree of hydrolysis is 24.9%.
For achieving the above object, the present invention adopts following technical scheme: a kind of is the method for raw material production step-down peptide with the Ovum Gallus domesticus album, it is characterized in that, Ovum Gallus domesticus album was mixed with volume ratio with edible vinegar in 2: 1～1: 2, and the total acidity scope of edible vinegar is 5～15g/100mL, after stirring, place 6～10h in 30 ℃～40 ℃, ultra-filtration membrane between employing 10kDa～1kDa is in 25～45 ℃, and 0.1MPa～0.2MPa is to feed liquid ultrafiltration 10min～30min, and ultrafiltration sees through liquid and passes through H more respectively
+Zeo-karb and OH
-Anionite-exchange resin.The effluent liquid of collecting be the ACE inhibiting rate can reach 89.2% be the step-down peptide of raw material production with the Ovum Gallus domesticus album.
Ovum Gallus domesticus album mixed with volume ratio with edible vinegar in 2: 1～1: 2.
The total acidity scope of edible vinegar is 5～15g/100mL.
After the mixing liquid of Ovum Gallus domesticus album and edible vinegar stirs, place 6～10h in 3 ℃ 0～40 ℃.
Ultra-filtration membrane between employing 10kDa～1kDa is in 25～45 ℃, and 0.1MPa～0.2MPa is to feed liquid ultrafiltration 10min～30min.
Ultrafiltration sees through liquid and passes through H more respectively
+Zeo-karb and OH
-From cloudy sub-exchange resin.It is the step-down peptide of raw material production that the effluent liquid of collecting is with the Ovum Gallus domesticus album.
Embodiment
Embodiment 1: Ovum Gallus domesticus album is mixed with volume ratio with edible vinegar at 2: 1, and the total acidity of edible vinegar is 5g/100mL, and after stirring, in 40 ℃ of placement 10h, the ultra-filtration membrane that adopts 8kDa is in 30 ℃, and 0.1MPa is to feed liquid ultrafiltration 20min, and ultrafiltration sees through liquid and passes through H more respectively
+Zeo-karb and OH
-Anionite-exchange resin.Effluent liquid be the ACE inhibiting rate can reach 89.2% be the step-down peptide of raw material production with the Ovum Gallus domesticus album.
Embodiment 2: Ovum Gallus domesticus album is mixed with volume ratio with edible vinegar at 1: 1, and the total acidity scope of edible vinegar is 10g/100mL, after stirring, place 8h in 35 ℃, the ultra-filtration membrane that adopts 6kDa is in 25 ℃, and 0.2MPa is to feed liquid ultrafiltration 30min, and ultrafiltration sees through liquid and 8 passes through H respectively again
+Zeo-karb and OH
-Anionite-exchange resin.Effluent liquid be the ACE inhibiting rate can reach 89.2% be the step-down peptide of raw material production with the Ovum Gallus domesticus album.
Embodiment 3: Ovum Gallus domesticus album is mixed with volume ratio with edible vinegar at 1: 2, and the total acidity scope of edible vinegar is 5～15g/100mL, after stirring, place 6h in 30 ℃, the ultra-filtration membrane that adopts 10kDa is in 35 ℃, and 0.2MPa is to feed liquid ultrafiltration 20min, and ultrafiltration sees through liquid and passes through H more respectively
+Zeo-karb and OH
-Anionite-exchange resin.Effluent liquid be the ACE inhibiting rate can reach 89.2% be the step-down peptide of raw material production with the Ovum Gallus domesticus album.
Claims (6)
1. one kind is the method for raw material production step-down peptide with the Ovum Gallus domesticus album, it is characterized in that, Ovum Gallus domesticus album was mixed with volume ratio with edible vinegar in 2: 1～1: 2, the total acidity scope of edible vinegar is 5～15g/100mL, after stirring, in 30 ℃～40 ℃ placement 6～10h, the ultra-filtration membrane between employing 10kDa～1kDa is in 25～45 ℃, 0.1MPa～0.2MPa is to feed liquid ultrafiltration 10min～30min, ultrafiltration sees through liquid and passes through H more respectively
+Zeo-karb and OH
-Anionite-exchange resin.The effluent liquid of collecting be the ACE inhibiting rate can reach 89.2% be the step-down peptide of raw material production with the Ovum Gallus domesticus album.
2. preparation method as claimed in claim 1 is characterized in that, Ovum Gallus domesticus album mixed with volume ratio with edible vinegar in 2: 1～1: 2.
3. preparation method as claimed in claim 1 is characterized in that, the total acidity scope of edible vinegar is 5～15g/100mL.
4. preparation method as claimed in claim 1 after the mixing liquid of Ovum Gallus domesticus album and edible vinegar stirs, places 6～10h in 3 ℃ 0～40 ℃.
5. preparation method as claimed in claim 1 is characterized in that, the ultra-filtration membrane between employing 10kDa～1kDa is in 25～45 ℃, and 0.1MPa～0.2MPa is to feed liquid ultrafiltration 10min～30min.
6. preparation method as claimed in claim 1 is characterized in that, ultrafiltration sees through liquid and passes through H more respectively
+Zeo-karb and OH
-From cloudy sub-exchange resin.It is the step-down peptide of raw material production that the effluent liquid of collecting is with the Ovum Gallus domesticus album.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010556411 CN102115490A (en) | 2010-11-24 | 2010-11-24 | Method for producing blood pressure depressed peptide by using egg white as raw material |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201010556411 CN102115490A (en) | 2010-11-24 | 2010-11-24 | Method for producing blood pressure depressed peptide by using egg white as raw material |
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| CN102115490A true CN102115490A (en) | 2011-07-06 |
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| CN 201010556411 Pending CN102115490A (en) | 2010-11-24 | 2010-11-24 | Method for producing blood pressure depressed peptide by using egg white as raw material |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104814439A (en) * | 2015-05-27 | 2015-08-05 | 吉林大学 | Egg white mini-peptide antihypertensive nutrition powder and preparation method thereof |
| CN108929370A (en) * | 2018-07-15 | 2018-12-04 | 渤海大学 | Three kinds of egg source activity peptides with ACE inhibitory activity |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1814776A (en) * | 2005-11-10 | 2006-08-09 | 广西大学 | Egg yolk hypertension-reducing peptide and its preparing method |
| CN101130800A (en) * | 2007-09-01 | 2008-02-27 | 浙江大学 | Method for preparing antihypertensive peptide by enzymolysis of hairtail chine |
| CN101153310A (en) * | 2007-09-07 | 2008-04-02 | 浙江大学 | Method for producing anti-hypertension peptide with complex zymohydrolysis of hairtail backbone |
| CN101560242A (en) * | 2008-04-18 | 2009-10-21 | 天津天狮生物发展有限公司 | Separation and purification method for improving specific activity of antihypertensive peptide |
| CN101638682A (en) * | 2009-09-02 | 2010-02-03 | 吉林大学 | External detection method for reducing neurotensin activity |
-
2010
- 2010-11-24 CN CN 201010556411 patent/CN102115490A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1814776A (en) * | 2005-11-10 | 2006-08-09 | 广西大学 | Egg yolk hypertension-reducing peptide and its preparing method |
| CN101130800A (en) * | 2007-09-01 | 2008-02-27 | 浙江大学 | Method for preparing antihypertensive peptide by enzymolysis of hairtail chine |
| CN101153310A (en) * | 2007-09-07 | 2008-04-02 | 浙江大学 | Method for producing anti-hypertension peptide with complex zymohydrolysis of hairtail backbone |
| CN101560242A (en) * | 2008-04-18 | 2009-10-21 | 天津天狮生物发展有限公司 | Separation and purification method for improving specific activity of antihypertensive peptide |
| CN101638682A (en) * | 2009-09-02 | 2010-02-03 | 吉林大学 | External detection method for reducing neurotensin activity |
Non-Patent Citations (1)
| Title |
|---|
| 《食品科技》 20060820 廖丹葵等 碱性蛋白酶水解制备蛋黄降压肽工艺条件优化 , 第08期 2 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104814439A (en) * | 2015-05-27 | 2015-08-05 | 吉林大学 | Egg white mini-peptide antihypertensive nutrition powder and preparation method thereof |
| CN108929370A (en) * | 2018-07-15 | 2018-12-04 | 渤海大学 | Three kinds of egg source activity peptides with ACE inhibitory activity |
| CN108929370B (en) * | 2018-07-15 | 2021-06-25 | 渤海大学 | Three egg source active peptides with ACE (angiotensin converting enzyme) inhibitory activity |
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Application publication date: 20110706 |