CN102084932B - Preparation method of organic copper enzyme preparation - Google Patents
Preparation method of organic copper enzyme preparation Download PDFInfo
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- CN102084932B CN102084932B CN2009102311076A CN200910231107A CN102084932B CN 102084932 B CN102084932 B CN 102084932B CN 2009102311076 A CN2009102311076 A CN 2009102311076A CN 200910231107 A CN200910231107 A CN 200910231107A CN 102084932 B CN102084932 B CN 102084932B
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Abstract
The invention provides a preparation method of an organic copper enzyme preparation, which comprises the steps of: uniformly mixing raw materials, such as wheat bran, bean pulp, rice husk powder, a copper sulfate solution and the like to prepare a mixture, uniformly stirring, carrying out high-temperature high-pressure sterilization, inoculating a koji tray strain of candida utilis after sterilizing, uniformly stirring and then culturing, then taking materials obtained by the culturing out, inoculating a koji tray strain of aspergillus usamii, uniformly stirring and then subculturing, then taking materials obtained by the subculturing out, inoculating a koji tray strain of bacillus subtilis, uniformly stirring and then culturing for the third time, and drying and crushing materials obtained by the third-time culturing to prepare the organic copper enzyme preparation. The method has the advantages of simple preparation process and low production cost; three times of transformation are continually carried out by adopting the three stains, and inorganic copper is transformed into organic copper with high transformation rate reaching above 98 percent; and the prepared enzyme preparation integrates organic trace elements, an enzyme preparation and probiotics into a whole, has multiple biological functions, and can enhance the immunity of the fed animal.
Description
Technical field
The invention provides a kind of preparation method of organic copper enzyme preparation.
Background technology
At present, add the copper sulphate of high dose in some feed, although this makes the pig of raising the phenomenon that skin is red, hair is bright and ight soil is black occur.But medium-term and long-term this Inorganic Copper of use copper sulphate of feed, will contain Inorganic Copper in the ight soil of letting animals feed, with these ight soil, after farmland fertilization, will cause the copper in soil to be accumulated, and forms and pollute., in case these ight soil polluted sources, will cause water quality deterioration, cause aquatile dead.It is reported, with the animal wastes of the Inorganic Copper forage feed that adds high dose, to herbage, apply fertilizer, when the herbage of growth is fed sheep, can make sheep generation copper poisoning.The effective ways that address these problems are to adopt organic copper.So both can the full feeding animal to the needs of copper, make it the healthy growth breeding, can not work the mischief to environment again.Its reason is, organic copper is that the form with chelate or complex compound exists, its total electric charge is zero, the molecular structure stable chemical nature, generally not can with other material generation chemical reactions, easily see through mucous membrane of small intestine and be absorbed, therefore, can meet the needs of organism with 1/4～1/5 of Inorganic Copper consumption.And the complex manufacturing of existing organic copper, production cost is higher.
Summary of the invention
The present invention wants the technical solution problem to be, a kind of preparation method of organic copper enzyme preparation is provided, to reduce production costs.
the present invention realizes like this, a kind of preparation method of organic copper enzyme preparation, described method is, press wheat bran 81～85 weight portions, dregs of beans 11～13 weight portions, powdered rice hulls 0.9～1.1 weight portion, ammonium sulfate 0.4～0.6 weight portion, potassium dihydrogen phosphate 0.4～0.6 weight portion, Fructus Hordei Germinatus 2～4 weight portions, mass percent concentration is that the proportioning of 0.9～1.1% copper-bath 90～110 weight portions obtains compound with above-mentioned mixing of materials, after stirring, when mixture moisture content is not enough, with sterilized water to the compound supplementing water, making its water content is 55～65% by weight, then, be 0.18～0.22MPa at pressure, temperature is under the condition of 115～125 ℃, carry out the autoclave sterilization of 43～46 minutes, after sterilizing, when above-mentioned material is cooled to 36～38 ℃, inoculate the koji tray bacterial classification of 2.3～2.7% candida utili (Candida utilis) of its weight, cultivated 23～25 hours at 27～29 ℃ of temperature after stirring, then will cultivate the gained material takes out, with sterilized water to cultivating gained material supplementing water, making the water content of cultivating gains is 55～65% by weight, again on the cultivation gained material after supplementing water, inoculate the koji tray bacterial classification of 1.9～2.1% Aspergillus usamii (Aspergillususanii) of its weight, again cultivated 47～49 hours at 36～37 ℃ of temperature after stirring, then will again cultivate the gained material takes out, while again cultivating gained material moisture deficit, with sterilized water to again cultivating gained material supplementing water, making the water content of again cultivating gains is 55～65% by weight, on the gained of cultivation again material after again cultivating gained material or supplementing water, inoculate the koji tray bacterial classification of 1.9～2.1% bacillus subtilis (Bacillus sutilis) of its weight, cultivated for the third time 23～25 hours at 36～37 ℃ of temperature after stirring, to cultivate for the third time the gained drying materials, pulverize, cross 80～100 mesh sieves, make organic copper enzyme preparation.
The preparation method of this organic iron enzyme preparation, production technology is simple, production cost is low, adopting three kinds of bacterium to carry out continuous three times transforms, the conversion ratio that Inorganic Copper is converted into organic copper is high, can reach more than 98%, extremely low Inorganic Copper was converted into the higher organic copper of tiring with originally tiring, the enzyme preparation of making integrates organic trace element, enzyme preparation, probio, have both the various biological function, be a kind of highly active organocopper compound, be conducive to absorbing of letting animals feed, can strengthen the immunity of letting animals feed.And the smell that affects animal appetite that has occurred while having avoided adding Inorganic Copper in feed, have distinctive fragrance, obviously the appetite of letting animals feed.
The specific embodiment
Below further illustrate the present invention.
Described koji tray bacterial classification is also referred to as the plate bacterial classification, after to be bacterial classification change triangular flask over to and enlarge and cultivate from test tube, then changes koji tray over to from triangular flask and enlarges and cultivate the bacterial classification that obtains.
Through test, the organic copper enzyme preparation of the present invention's method preparation, can suppress the generation of small white mouse tumour significantly, shows that it is to being improved the effect of immunity of organisms, enhancing development for the examination animal.
Embodiment 1
a kind of preparation method of organic copper enzyme preparation, described method is, press wheat bran 81 weight portions, dregs of beans 13 weight portions, powdered rice hulls 0.9 weight portion, ammonium sulfate 0.6 weight portion, potassium dihydrogen phosphate 0.4 weight portion, Fructus Hordei Germinatus 4 weight portions, mass percent concentration is that the proportioning of 0.9% copper-bath 110 weight portions obtains compound with above-mentioned mixing of materials, after stirring, when mixture moisture content is not enough, with sterilized water to the compound supplementing water, making its water content is 65% by weight, then, be 0.18MPa at pressure, temperature is under the condition of 125 ℃, carry out the autoclave sterilization of 43 minutes, after sterilizing, when above-mentioned material is cooled to 38 ℃, inoculate the koji tray bacterial classification of 2.3% candida utili (Candida utilis) of its weight, cultivated 23 hours at 29 ℃ of temperature after stirring, then will cultivate the gained material takes out, with sterilized water to cultivating gained material supplementing water, making the water content of cultivating gains is 65% by weight, again on the cultivation gained material after supplementing water, inoculate the koji tray bacterial classification of 1.9% Aspergillus usamii (Aspergillus usanii) of its weight, again cultivated 47 hours at 37 ℃ of temperature after stirring, then will again cultivate the gained material takes out, while again cultivating gained material moisture deficit, with sterilized water to again cultivating gained material supplementing water, making the water content of again cultivating gains is 65% by weight, on the gained of cultivation again material after again cultivating gained material or supplementing water, inoculate the koji tray bacterial classification of 1.9% bacillus subtilis (Bacillus sutilis) of its weight, cultivated for the third time 23 hours at 37 ℃ of temperature after stirring, to cultivate for the third time the gained drying materials, pulverize, cross 100 mesh sieves, make organic copper enzyme preparation.
Embodiment 2
a kind of preparation method of organic copper enzyme preparation, described method is, press wheat bran 85 weight portions, dregs of beans 11 weight portions, powdered rice hulls 1.1 weight portions, ammonium sulfate 0.4 weight portion, potassium dihydrogen phosphate 0.6 weight portion, Fructus Hordei Germinatus 2 weight portions, mass percent concentration is that the proportioning of 1.1% copper-bath 90 weight portions obtains compound with above-mentioned mixing of materials, after stirring, when mixture moisture content is not enough, with sterilized water to the compound supplementing water, making its water content is 55% by weight, then, be 0.22MPa at pressure, temperature is under the condition of 115 ℃, carry out the autoclave sterilization of 46 minutes, after sterilizing, when above-mentioned material is cooled to 36 ℃, inoculate the koji tray bacterial classification of 2.7% candida utili (Candida utilis) of its weight, cultivated 25 hours at 27 ℃ of temperature after stirring, then will cultivate the gained material takes out, with sterilized water to cultivating gained material supplementing water, making the water content of cultivating gains is 55% by weight, again on the cultivation gained material after supplementing water, inoculate the koji tray bacterial classification of 2.1% Aspergillus usamii (Aspergillus usanii) of its weight, again cultivated 49 hours at 36 ℃ of temperature after stirring, then will again cultivate the gained material takes out, while again cultivating gained material moisture deficit, with sterilized water to again cultivating gained material supplementing water, making the water content of again cultivating gains is 55% by weight, on the gained of cultivation again material after again cultivating gained material or supplementing water, inoculate the koji tray bacterial classification of 2.1% bacillus subtilis (Bacillus sutilis) of its weight, cultivated for the third time 25 hours at 36 ℃ of temperature after stirring, to cultivate for the third time the gained drying materials, pulverize, cross 80 mesh sieves, make organic copper enzyme preparation.
Embodiment 3
a kind of preparation method of organic copper enzyme preparation, described method is, press wheat bran 83 weight portions, dregs of beans 12 weight portions, powdered rice hulls 1.0 weight portions, ammonium sulfate 0.5 weight portion, potassium dihydrogen phosphate 0.5 weight portion, Fructus Hordei Germinatus 3 weight portions, mass percent concentration is that the proportioning of 1.0% copper-bath 100 weight portions obtains compound with above-mentioned mixing of materials, after stirring, when mixture moisture content is not enough, with sterilized water to the compound supplementing water, making its water content is 60% by weight, then, be 0.20MPa at pressure, temperature is under the condition of 120 ℃, carry out the autoclave sterilization of 45 minutes, after sterilizing, when above-mentioned material is cooled to 37 ℃, inoculate the koji tray bacterial classification of 2.5% candida utili (Candida utilis) of its weight, cultivated 24 hours at 28 ℃ of temperature after stirring, then will cultivate the gained material takes out, with sterilized water to cultivating gained material supplementing water, making the water content of cultivating gains is 60% by weight, again on the cultivation gained material after supplementing water, inoculate the koji tray bacterial classification of 2.0% Aspergillus usamii (Aspergillus usanii) of its weight, again cultivated 48 hours at 36.5 ℃ of temperature after stirring, then will again cultivate the gained material takes out, while again cultivating gained material moisture deficit, with sterilized water to again cultivating gained material supplementing water, making the water content of again cultivating gains is 60% by weight, on the gained of cultivation again material after again cultivating gained material or supplementing water, inoculate the koji tray bacterial classification of 2.0% bacillus subtilis (Bacillus sutilis) of its weight, cultivated for the third time 24 hours at 36.5 ℃ of temperature after stirring, to cultivate for the third time the gained drying materials, pulverize, cross 90 mesh sieves, make organic copper enzyme preparation.
Claims (1)
1. the preparation method of an organic copper enzyme preparation, it is characterized in that, described method is, press wheat bran 81～85 weight portions, dregs of beans 11～13 weight portions, powdered rice hulls 0.9～1.1 weight portion, ammonium sulfate 0.4～0.6 weight portion, potassium dihydrogen phosphate 0.4～0.6 weight portion, Fructus Hordei Germinatus 2～4 weight portions, mass percent concentration is that the proportioning of 0.9～1.1% copper-bath 90～110 weight portions obtains compound with above-mentioned mixing of materials, after stirring, when mixture moisture content is not enough, with sterilized water to the compound supplementing water, making its water content is 55～65% by weight, then, be 0.18～0.22MPa at pressure, temperature is under the condition of 115～125 ℃, carry out the autoclave sterilization of 43～46 minutes, after sterilizing, when above-mentioned material is cooled to 36～38 ℃, inoculate the koji tray bacterial classification of 2.3～2.7% candida utili (Candida utilis) of its weight, cultivated 23～25 hours at 27～29 ℃ of temperature after stirring, then will cultivate the gained material takes out, with sterilized water to cultivating gained material supplementing water, making the water content of cultivating gains is 55～65% by weight, again on the cultivation gained material after supplementing water, inoculate the koji tray bacterial classification of 1.9～2.1% Aspergillus usamii (Aspergillus usanii) of its weight, again cultivated 47～49 hours at 36～37 ℃ of temperature after stirring, then will again cultivate the gained material takes out, while again cultivating gained material moisture deficit, with sterilized water to again cultivating gained material supplementing water, making the water content of again cultivating gains is 55～65% by weight, on the gained of cultivation again material after again cultivating gained material or supplementing water, inoculate the koji tray bacterial classification of 1.9～2.1% bacillus subtilis (Bacillus sutilis) of its weight, cultivated for the third time 23～25 hours at 36～37 ℃ of temperature after stirring, to cultivate for the third time the gained drying materials, pulverize, cross 80～100 mesh sieves, make organic copper enzyme preparation.
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| CN2009102311076A CN102084932B (en) | 2009-12-04 | 2009-12-04 | Preparation method of organic copper enzyme preparation |
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| CN2009102311076A CN102084932B (en) | 2009-12-04 | 2009-12-04 | Preparation method of organic copper enzyme preparation |
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| CN102084932A CN102084932A (en) | 2011-06-08 |
| CN102084932B true CN102084932B (en) | 2013-11-13 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1304912A (en) * | 2000-12-31 | 2001-07-25 | 李承海 | Process for preparing microecological organic fertilizer |
| CN1570082A (en) * | 2004-04-27 | 2005-01-26 | 包头市天赋食品有限公司 | Multiple strain microorganism ferment production method and its uses in cow fine fodder |
| CN1682599A (en) * | 2004-04-14 | 2005-10-19 | 中国农业大学 | Microbial fodder additive and its preparing method and use |
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- 2009-12-04 CN CN2009102311076A patent/CN102084932B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1304912A (en) * | 2000-12-31 | 2001-07-25 | 李承海 | Process for preparing microecological organic fertilizer |
| CN1682599A (en) * | 2004-04-14 | 2005-10-19 | 中国农业大学 | Microbial fodder additive and its preparing method and use |
| CN1570082A (en) * | 2004-04-27 | 2005-01-26 | 包头市天赋食品有限公司 | Multiple strain microorganism ferment production method and its uses in cow fine fodder |
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| CN102084932A (en) | 2011-06-08 |
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