CN102070724A - Method for separating neutral longan polysaccharide fraction - Google Patents
Method for separating neutral longan polysaccharide fraction Download PDFInfo
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Abstract
The invention discloses a method for separating a neutral longan polysaccharide fraction. The method comprises the following steps of: (1) selecting longan fruit pulp water extract, and removing most of protein and pigment from the longan fruit pulp water extract through D301-F anion exchange resin to obtain crude longan polysaccharide; (2) separating and purifying the crude longan polysaccharide by a diethylaminoethyl DEAE-52 cellulose ion exchange chromatographic column, eluting with deionized water, collecting longan polysaccharide eluent, concentrating and freeze-drying under vacuum to obtain neutral longan polysaccharide powder; and (3) allowing the neutral longan polysaccharide to pass through a SephadexG-100 gel permeation chromatographic column, eluting with the deionized water, collecting different kinds of longan polysaccharide fraction eluent, concentrating and freeze-drying under vacuum to obtain the neutral longan polysaccharide fraction. By the method, a neutral single polysaccharide component can be separated and prepared.
Description
Technical field
The invention belongs to technical field of agricultural product process, be specifically related to a kind of separation method of neutral longan polysaccharide fraction.
Background technology
Longan mainly is distributed in the ground such as Guangdong, Guangxi, Fujian and Taiwan of China, is typical subtropics cash crop.Longan is the medicine food dual purpose plant that health ministry is announced, records " the money benefit is good with the longan " in the Compendium of Material Medica, and points out that longan aril has the effect of " Appetizing spleen-tonifying, qi-restoratives intelligence development ".And longan pulp is usually used in treating symptoms such as deficiency of heart-blood, severe palpitation, insomnia forgetfulness, anaemia, diarrhea due to hypofunction of the spleen among the people.
At present, the method for separating and preparing of longan polysaccharide is mainly water extract-alcohol precipitation, generally can remove impurity such as protein in the longan pulp water extracts and pigment by technology such as Sevag method, dialysis and alcohol precipitation, but be difficult to reach higher purity.Total sugar content is 64.4% in the longan polysaccharide that obtains as separation such as Jia Qi, and the longan polysaccharide purity that separation such as Ling Xueping obtain also only reaches 80.96%.Ion exchange chromatography can effectively separate neutral component and the acidic components in the Crude polysaccharides, can realize the monomers and polysaccharide separation of different molecular weight again through gel permeation chromatography.The longan polysaccharide of confirmation microwave pre-treatment-hot water lixiviates such as Yang Cuixian is an acid heteroglycan, and Jia Qi etc. obtain a kind of acid longan polysaccharide homogeneous component in conjunction with DEAE-Mierocrystalline cellulose and SephacrylS-300 column chromatography.But about the also rarely seen report of the separation and purification of longan polysaccharide neutral component.
Summary of the invention
The object of the present invention is to provide a kind of separation method of neutral longan polysaccharide fraction, can obtain the neutral longan polysaccharide of one-component by this method.
Above-mentioned purpose of the present invention can realize by following technical solution: a kind of separation method of neutral longan polysaccharide fraction contains following steps:
(1) chooses the longan pulp water extract, the longan pulp water extract is removed most of protein and pigment obtains the longan Crude polysaccharides through D301-F anionite-exchange resin;
(2) the longan Crude polysaccharides is adopted DEAE-52 cellulose ion-exchange chromatography column separating purification,, collect the longan polysaccharide elutriant, concentrate with vacuum lyophilization and get neutral longan polysaccharide powder with the deionized water wash-out;
(3) with neutral longan polysaccharide through SephadexG-100 gel permeation chromatography post, with the deionized water wash-out, collect different longan polysaccharide fractions elutriant, concentrate and vacuum lyophilization gets neutral longan polysaccharide fraction.
In above-mentioned steps:
Longan pulp aqueous extract described in the step (1) prepares as follows: select fresh longan raw material, dry, remove peeling and nuclear, pulverize, get longan pulp; In longan pulp, add the water of 30-40 times of weight, be heated to 70-80 ℃, place lixiviate 30-50min under the ultrasonic intensity of 200-300W, cancel ultrasonic continuation lixiviate 100-120min; Vat liquor is after filtration with after the pulp residue separates, with filtrate in 60-70 ℃ of following vacuum concentration to little thick, collect the concentrated solution vacuum lyophilization and promptly get the longan pulp aqueous extract.
The concrete preparation process of the longan Crude polysaccharides described in the step (1) is: add entry and D301-F anionite-exchange resin in the longan pulp aqueous extract, the rotating speed of regulator solution pH value, temperature and vibrator, after the vibration, filter and collect longan Crude polysaccharides solution, get the longan Crude polysaccharides through vacuum concentration and vacuum lyophilization again.
Parameters in the step (1) in the longan Crude polysaccharides preparation process is: the consumption of water is 20-30 a times of longan pulp extract, the consumption of D301-F anionite-exchange resin is 3-4 a times of longan pulp water extract, regulator solution pH value is 5.0-6.0, temperature is 45-55 ℃, and regulating the vibrator rotating speed is 100-120r/min vibration 2-3h.Separation solution, is collected the concentrated solution vacuum lyophilization and is promptly got the longan Crude polysaccharides to little thick in 55-65 ℃ of following vacuum concentration.
D301-F anionite-exchange resin described in the step (1) needs through pre-treatment, and preprocessing process is: through the water logging bubble, removing upper strata suspended particle and impurity earlier, is the NaCl solution soaking 3-4h after washing of 4-5% again with weight percentage; Be the NaOH solution soaking 3-4h of 4-5% then, be washed to nearly pH neutral, last 50-55 ℃ of dry for standby with weight percentage.
The condition of DEAE-52 cellulose ion-exchange chromatography column separating purification is in the step (2): effective column volume of described DEAE-52 cellulose ion-exchange chromatography post is 30-210mL, and it is that polysaccharide/effectively column volume is about 1.0mg/mL that described DEAE-52 cellulose ion-exchange chromatography post effectively exchanges volume; Effective column volume of described DEAE-52 cellulose ion-exchange chromatography post and sample introduction liquor capacity ratio are 6.0-8.0: 1; The washed with de-ionized water volume is 2-3 with the effective column volume ratio of the described DEAE-52 cellulose ion-exchange chromatography of DEAE-52 cellulose ion-exchange chromatography post post: 1; Eluent flow rate (mL/min) is 1 with the ratio of effective column volume (L): 3-4.
DEAE-52 cellulose ion-exchange chromatography post described in the step (2) needs through pre-treatment, and described preprocessing process is: the DEAE-52 filler soaks through deionized water, removes upper strata suspended particle and impurity; With 0.4-0.5mol/L NaOH solution soaking 30-40min, be washed to nearly pH neutral; With 0.4-0.5mol/L HCl solution soaking 30-40min, be washed to nearly pH neutral; With 0.4-0.5mol/LNaOH solution soaking 30-40min, be washed to nearly pH neutral, adopt wet method dress post promptly to get OH
-The type chromatography column.
The condition that SephadexG-100 gel permeation chromatography post described in the step (3) separates the longan polysaccharide fraction is: effective column volume 30-100mL of the SephadexG-100 gel permeation chromatography post of choosing; The sample introduction concentration of polysaccharide is 2-8mg/mL; Effective column volume of SephadexG-100 gel permeation chromatography post and sample introduction liquor capacity ratio are 20-30: 1; Deionized water elution flow rate (mL/min) is 3-4 with the ratio of effective column volume (L): 1.
SephadexG-100 gel permeation chromatography post described in the step (3) needs through pre-treatment, described preprocessing process is: the SephadexG-100 filler is soaked through deionized water, remove upper strata suspended particle and impurity, and, adopt wet method dress post promptly to get the SephadexG-100 gel chromatography column with boiling water treating 25-35min.
In the step (2) that the longan polysaccharide elutriant is extremely little thick at 55-65 ℃ of following vacuum concentration, collect the concentrated solution vacuum lyophilization and promptly get Powdered longan polysaccharide; In the step (3) that longan polysaccharide fraction elutriant is extremely little thick at 55-65 ℃ of following vacuum concentration, collect the concentrated solution vacuum lyophilization and promptly be prepared into neutral longan protein-polysaccharide fraction.
The advantage that the present invention has is: the present invention carries out separation and purification to the longan Crude polysaccharides, obtains neutral polysaccharide one-component.This polysaccharide mainly is made up of glucose and seminose, and molecular weight is about 1.4 * 10
4G/moL, and have certain immunoregulatory activity, for new approach has been opened up in the intensive processing of longan.
Description of drawings
The present invention is described in further detail below in conjunction with the drawings and specific embodiments.
Fig. 1 is the elution curve of the neutral longan polysaccharide fraction of preparation among the embodiment 1;
Fig. 2 is the elution curve of the neutral longan polysaccharide fraction of preparation among the embodiment 2;
Fig. 3 is the elution curve of the neutral longan polysaccharide fraction of preparation among the embodiment 3.
Embodiment
Select fresh longan raw material, dry, remove peeling and nuclear, pulverize, get longan pulp; In longan pulp, add the water of 30 times of weight, be heated to 80 ℃, place lixiviate 30min under the ultrasonic intensity of 300W, cancel ultrasonic continuation lixiviate 120min; Vat liquor is after filtration with after the pulp residue separates, and 60 ℃ of following vacuum concentration are collected the concentrated solution vacuum lyophilization and promptly got the longan pulp aqueous extract to little thick.
D301-F anionite-exchange resin through the water logging bubble, removes upper strata suspended particle and impurity earlier, is 4% NaCl solution soaking 4h after washing again with weight percentage; Then be 4% NaOH solution soaking 4h, be washed to nearly pH neutral, last 50 ℃ of dry for standby with weight percentage.
The longan pulp extract is dissolved in the water of 20 times of weight, adds the D301-F anionite-exchange resin of 3 times of longan pulp water extract weight, regulator solution pH value is 5.0, and temperature is 55 ℃, and regulating the vibrator rotating speed is 120r/min vibration 3h.Separation solution, is collected the concentrated solution vacuum lyophilization and is promptly got the longan Crude polysaccharides to little thick in 55 ℃ of following vacuum concentration.
The DEAE-52 filler soaks through deionized water, removes upper strata suspended particle and impurity; With 0.4mol/L NaOH solution soaking 40min, be washed to nearly pH neutral; With 0.4mol/L HCl solution soaking 40min, be washed to nearly pH neutral; With 0.4mol/L NaOH solution soaking 40min, be washed to nearly pH neutral, adopt wet method to be loaded on (effectively column volume is about 210mL) in 2.5 * 50cm medium pressure chromatography post, and adopt deionized water with 0.60mL/min flow velocity balance 1 day.
Get 210mg longan Crude polysaccharides and be dissolved in the 35mL deionized water, the centrifugal 10min of 4500rmp gets the supernatant liquor sample introduction; Cross DEAE-52 cellulose ion-exchange chromatography post, with 0.60mL/min flow velocity wash-out, adopt polysaccharide in the phenol sulfuric acid process monitoring elutriant with deionized water, being positive from reaction begins to collect elutriant to reaction and is negative; Elutriant is extremely little thick at 55 ℃ of following vacuum concentration, collect the concentrated solution vacuum lyophilization and promptly get Powdered neutral longan polysaccharide powder.
The SephadexG-100 filler soaks through deionized water, remove upper strata suspended particle and impurity, and with the about 30min of boiling water treating, wet method is loaded in 1.6 * 60cm medium pressure chromatography post (effectively column volume is about 100mL) and adopts deionized water with 0.30mL/min flow velocity balance 1 day.
Get the neutral longan polysaccharide of 40mg and be dissolved in (concentration is 8mg/mL, and effectively column volume and sample introduction liquor capacity ratio are 20: 1) in the 5mL deionized water, the centrifugal 10min of 4500rmp gets the supernatant liquor sample introduction; With 0.30mL/min flow velocity wash-out (elution flow rate with effectively the ratio of column volume (L) be 3: 1), adopt polysaccharide in the phenol sulfuric acid process monitoring elutriant with deionization, being positive from reaction begins to collect elutriant to reaction and is negative; Different polysaccharide fractions elutriants are extremely little thick at 55 ℃ of following vacuum concentration respectively, collect the concentrated solution vacuum lyophilization and promptly get neutral longan polysaccharide fraction.
Select fresh longan raw material, dry, remove peeling and nuclear, pulverize, get longan pulp; In longan pulp, add the water of 35 times of weight, be heated to 75 ℃, place lixiviate 40min under the ultrasonic intensity of 250W, cancel ultrasonic continuation lixiviate 110min; Vat liquor is after filtration with after the pulp residue separates, and 65 ℃ of following vacuum concentration are collected the concentrated solution vacuum lyophilization and promptly got the longan pulp aqueous extract to little thick.
D301-F anionite-exchange resin through the water logging bubble, removes upper strata suspended particle and impurity earlier, is 4.5% NaCl solution soaking 3.5h after washing again with weight percentage; Then be 4.5% NaOH solution soaking 3.5h, be washed to nearly pH neutral, last 52.5 ℃ of dry for standby with weight percentage.
The longan pulp extract is dissolved in the water of 25 times of weight, adds the D301-F anionite-exchange resin of 3.5 times of longan pulp water extract weight, regulator solution pH value is 5.5, and temperature is 50 ℃, and regulating the vibrator rotating speed is 110r/min vibration 2.5h.Separation solution, is collected the concentrated solution vacuum lyophilization and is promptly got the longan Crude polysaccharides to little thick in 60 ℃ of following vacuum concentration.
The DEAE-52 filler soaks through deionized water, removes upper strata suspended particle and impurity; With 0.45mol/L NaOH solution soaking 35min, be washed to nearly pH neutral; With 0.45mol/L HCl solution soaking 35min, be washed to nearly pH neutral; With 0.45mol/L NaOH solution soaking 35min, be washed to nearly pH neutral, adopt wet method to be loaded on (effectively column volume is about 120mL) in 2.0 * 40cm medium pressure chromatography post, and adopt deionized water with 0.36mL/min flow velocity balance 1 day.
Get 120mg longan Crude polysaccharides and be dissolved in the 15mL deionized water, the centrifugal 10min of 4500rmp gets the supernatant liquor sample introduction, crosses DEAE-52 cellulose ion-exchange chromatography post; With 0.36mL/min flow velocity wash-out, adopt polysaccharide in the phenol sulfuric acid process monitoring elutriant with deionized water, being positive from reaction begins to collect elutriant to reaction and is negative; The polysaccharide elutriant, is collected the concentrated solution vacuum lyophilization and is promptly got Powdered neutral longan polysaccharide to little thick at 55 ℃ of following vacuum concentration.
The SephadexG-100 filler soaks through deionized water, remove upper strata suspended particle and impurity, and with the about 30min of boiling water treating, wet method is loaded in 1.6 * 40cm medium pressure chromatography post (effectively column volume is about 65mL) and adopts deionized water with 0.23mL/min flow velocity balance 1 day.
Get the neutral longan polysaccharide of 13mg and be dissolved in (concentration is 5mg/mL, and effectively column volume and sample introduction liquor capacity ratio are 25: 1) in the 2.6mL deionized water, the centrifugal 10min of 4500rmp gets the supernatant liquor sample introduction; With 0.23mL/min flow velocity wash-out (elution flow rate with effectively the ratio of column volume (L) be 3.5: 1), adopt polysaccharide in the phenol sulfuric acid process monitoring elutriant with deionization, being positive from reaction begins to collect elutriant to reaction and is negative; Different polysaccharide fractions elutriants are extremely little thick at 55 ℃ of following vacuum concentration respectively, collect the concentrated solution vacuum lyophilization and promptly get neutral longan polysaccharide fraction.
Embodiment 3
Select fresh longan raw material, dry, remove peeling and nuclear, pulverize, get longan pulp; In longan pulp, add the water of 40 times of weight, be heated to 70 ℃, place lixiviate 50min under the ultrasonic intensity of 200W, cancel ultrasonic continuation lixiviate 100min; Vat liquor is after filtration with after the pulp residue separates, and 70 ℃ of following vacuum concentration are collected the concentrated solution vacuum lyophilization and promptly got the longan pulp aqueous extract to little thick.
D301-F anionite-exchange resin through the water logging bubble, removes upper strata suspended particle and impurity earlier, is 5% NaCl solution soaking 3h after washing again with weight percentage; Then be 5% NaOH solution soaking 3h, be washed to nearly pH neutral, last 55 ℃ of dry for standby with weight percentage.
The longan pulp extract is dissolved in the water of 30 times of weight, adds the D301-F anionite-exchange resin of 4 times of longan pulp water extracts, regulator solution pH value is 6.0, and temperature is 45 ℃, regulates the vibrator rotating speed and be the 100r/min 2h that vibrates.Separation solution, is collected the concentrated solution vacuum lyophilization and is promptly got the longan Crude polysaccharides to little thick in 65 ℃ of following vacuum concentration.
The DEAE-52 filler soaks through deionized water, removes upper strata suspended particle and impurity; With 0.50mol/L NaOH solution soaking 30min, be washed to nearly pH neutral; With 0.50mol/L HCl solution soaking 30min, be washed to nearly pH neutral; With 0.50mol/L NaOH solution soaking 30min, be washed to nearly pH neutral, adopt wet method to be loaded on (effectively column volume is about 30mL) in 1.5 * 20cm medium pressure chromatography post, and adopt deionized water with 0.12mL/min flow velocity balance 1 day.
Get 30mg longan Crude polysaccharides and be dissolved in the 4mL deionized water, the centrifugal 10min of 4500rmp gets the supernatant liquor sample introduction, crosses DEAE-52 cellulose ion-exchange chromatography post; With 0.12mL/min flow velocity wash-out, adopt polysaccharide in the phenol sulfuric acid process monitoring elutriant with deionized water, being positive from reaction begins to collect elutriant to reaction and is negative; The polysaccharide elutriant, is collected the concentrated solution vacuum lyophilization and is promptly got Powdered neutral longan polysaccharide to little thick at 55 ℃ of following vacuum concentration.
The SephadexG-100 filler soaks through deionized water, remove upper strata suspended particle and impurity, and with boiling water treating 30min, wet method is loaded in 1.6 * 30cm medium pressure chromatography post (effectively column volume is about 30mL) and adopts deionized water with 0.12mL/min flow velocity balance 1 day.
Get the neutral longan polysaccharide of 2mg and be dissolved in (concentration is 2mg/mL, and effectively column volume and sample introduction liquor capacity ratio are 30: 1) in the 1mL deionized water, the centrifugal 10min of 4500rmp gets the supernatant liquor sample introduction; With 0.12mL/min flow velocity wash-out (elution flow rate with effectively the ratio of column volume (L) be 4: 1), adopt polysaccharide in the phenol sulfuric acid process monitoring elutriant with deionization, being positive from reaction begins to collect elutriant to reaction and is negative; Different polysaccharide fractions elutriants are extremely little thick at 55 ℃ of following vacuum concentration respectively, collect the concentrated solution vacuum lyophilization and promptly get neutral longan polysaccharide fraction.
The foregoing description is a preferred implementation of the present invention; but embodiments of the present invention are not restricted to the described embodiments; other any do not deviate from change, the modification done under spirit of the present invention and the principle, substitutes, combination, simplify; all should be the substitute mode of equivalence, be included in protection scope of the present invention.
Claims (10)
1. the separation method of a neutral longan polysaccharide fraction is characterized in that containing following steps:
(1) chooses the longan pulp water extract, the longan pulp water extract is removed most of protein and pigment obtains the longan Crude polysaccharides through D301-F anionite-exchange resin;
(2) the longan Crude polysaccharides is adopted DEAE-52 cellulose ion-exchange chromatography column separating purification,, collect the longan polysaccharide elutriant, concentrate with vacuum lyophilization and get neutral longan polysaccharide powder with the deionized water wash-out;
(3) with neutral longan polysaccharide through SephadexG-100 gel permeation chromatography post, with the deionized water wash-out, collect different longan polysaccharide fractions elutriant, concentrate and vacuum lyophilization gets neutral longan polysaccharide fraction.
2. the separation method of neutral longan polysaccharide fraction according to claim 1, it is characterized in that: the longan pulp aqueous extract described in the step (1) prepares as follows: select fresh longan raw material, oven dry removes peeling and nuclear, pulverize, get longan pulp; In longan pulp, add the water of 30-40 times of weight, be heated to 70-80 ℃, place lixiviate 30-50min under the ultrasonic intensity of 200-300W, cancel ultrasonic continuation lixiviate 100-120min; Vat liquor is after filtration with after the pulp residue separates, with filtrate in 60-70 ℃ of following vacuum concentration to little thick, collect the concentrated solution vacuum lyophilization and promptly get the longan pulp aqueous extract.
3. the separation method of neutral longan polysaccharide fraction according to claim 1, it is characterized in that: the concrete preparation process of the longan Crude polysaccharides described in the step (1) is: add entry and D301-F anionite-exchange resin in the longan pulp aqueous extract, the rotating speed of regulator solution pH value, temperature and vibrator, after the vibration, filter and collect longan Crude polysaccharides solution, get the longan Crude polysaccharides through vacuum concentration and vacuum lyophilization again.
4. the separation method of neutral longan polysaccharide fraction according to claim 3, it is characterized in that: the parameters in the longan Crude polysaccharides preparation process is: the consumption of water is 20-30 a times of longan pulp water extract gross weight, the consumption of D301-F anionite-exchange resin is 3-4 a times of longan pulp water extract gross weight, the pH value of regulator solution is 5.0-6.0, temperature is 45-55 ℃, the rotating speed 100-120r/min vibration 2-3h of vibrator, separation solution, is collected the concentrated solution vacuum lyophilization and is promptly got the longan Crude polysaccharides to little thick in 55-65 ℃ of following vacuum concentration.
5. the separation method of neutral longan polysaccharide fraction according to claim 1, it is characterized in that: the D301-F anionite-exchange resin described in the step (1) needs through pre-treatment, preprocessing process is: the D301-F filler is steeped through water logging, remove upper strata suspended particle and impurity; With weight percentage is the NaCl solution soaking 3-4h after washing of 4-5%; With weight percentage is the NaOH solution soaking 3-4h of 4-5%, is washed to nearly neutrality, 50-55 ℃ of dry for standby.
6. the separation method of neutral longan polysaccharide fraction according to claim 1, it is characterized in that: the condition that the middle DEAE-52 cellulose ion-exchange chromatography post of step (2) separates neutral longan polysaccharide is: effective column volume of described DEAE-52 cellulose ion-exchange chromatography post is 30-210mL, and effective exchange volume of DEAE-52 cellulose ion-exchange chromatography post is that polysaccharide/effectively column volume is 1.0mg/mL; Effective column volume of DEAE-52 cellulose ion-exchange chromatography post and sample introduction liquor capacity ratio are 6.0-8.0: 1; The washed with de-ionized water volume is 2-3 with the effective column volume ratio of the described DEAE-52 cellulose ion-exchange chromatography of DEAE-52 cellulose ion-exchange chromatography post post: 1; Eluent flow rate is 1 with the ratio of effective column volume: 3-4.
7. the separation method of neutral longan polysaccharide fraction according to claim 1, it is characterized in that: the DEAE-52 Mierocrystalline cellulose chromatography post described in the step (2) needs through pre-treatment, preprocessing process is: the DEAE-52 filler soaks through deionized water, removes upper strata suspended particle and impurity; With 0.4-0.5mol/L NaOH solution soaking 30-40min, be washed to nearly neutrality; With 0.4-0.5mol/L HCl solution soaking 30-40min, be washed to nearly neutrality; With 0.4-0.5mol/LNaOH solution soaking 30-40min, be washed to nearly neutrality, adopt wet method dress post promptly to get the DEAE-52 Mierocrystalline cellulose chromatography post of OH-type.
8. the preparation method of neutral longan polysaccharide fraction according to claim 1 is characterized in that: the condition that the SephadexG-100 gel permeation chromatography post described in the step (3) separates the longan polysaccharide fraction is: effective column volume 30-100mL of the SephadexG-100 gel permeation chromatography post of choosing; The sample introduction concentration of polysaccharide is 2-8mg/mL; Effective column volume of SephadexG-100 gel permeation chromatography post and sample introduction liquor capacity ratio are 20-30: 1; The deionized water elution flow rate is 3-4 with the ratio of effective column volume: 1.
9. the preparation method of neutral longan polysaccharide fraction according to claim 1, it is characterized in that: the SephadexG-100 gel permeation chromatography post described in the step (3) needs through pre-treatment, described preprocessing process is: the SephadexG-100 filler is soaked through deionized water, remove upper strata suspended particle and impurity, and, adopt wet method dress post promptly to get the SephadexG-100 gel chromatography column with boiling water treating 25-35min.
10. the preparation method of neutral longan polysaccharide fraction according to claim 1 is characterized in that: in the step (2) that the longan polysaccharide elutriant is extremely little thick at 55-65 ℃ of following vacuum concentration, and collect the concentrated solution vacuum lyophilization and promptly get Powdered longan polysaccharide; In the step (3) that longan polysaccharide fraction elutriant is extremely little thick at 55-65 ℃ of following vacuum concentration, collect the concentrated solution vacuum lyophilization and promptly be prepared into neutral longan protein-polysaccharide fraction.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104865333A (en) * | 2015-05-14 | 2015-08-26 | 武汉轻工大学 | Detection method of longan meat protein polysaccharide |
| CN107987177A (en) * | 2017-11-30 | 2018-05-04 | 广西壮族自治区农业科学院农产品加工研究所 | A kind of isolation and purification method of hypoglycemic Arillus longan polysaccharide |
| CN108003255A (en) * | 2017-12-22 | 2018-05-08 | 茂名市水果科学研究所 | A kind of longan polysaccharide extracting method |
| CN108948220A (en) * | 2018-06-28 | 2018-12-07 | 广东省农业科学院蚕业与农产品加工研究所 | A kind of low viscosity, the preparation method of the prebiotic active polysaccharide of highly dissoluble longan |
| CN116606385A (en) * | 2023-04-28 | 2023-08-18 | 广州工程技术职业学院 | Zedoary turmeric polysaccharide and preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1270963A (en) * | 2000-05-18 | 2000-10-25 | 厦门中卫多糖研究所 | Longan polysaccharide extracting process |
| CN1613878A (en) * | 2004-09-21 | 2005-05-11 | 福建省莆田市龙升生物技术有限公司 | Process of euphoria polysaccharide |
| CN101433620A (en) * | 2008-12-10 | 2009-05-20 | 广东省农业科学院农业生物技术研究所 | Longan water-soluble active substance as well as extraction method and use thereof |
-
2010
- 2010-12-24 CN CN2010106036865A patent/CN102070724B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1270963A (en) * | 2000-05-18 | 2000-10-25 | 厦门中卫多糖研究所 | Longan polysaccharide extracting process |
| CN1613878A (en) * | 2004-09-21 | 2005-05-11 | 福建省莆田市龙升生物技术有限公司 | Process of euphoria polysaccharide |
| CN101433620A (en) * | 2008-12-10 | 2009-05-20 | 广东省农业科学院农业生物技术研究所 | Longan water-soluble active substance as well as extraction method and use thereof |
Non-Patent Citations (2)
| Title |
|---|
| 《Food Chemistry》 20080915 Bao Yang等 "Optimization of tyrosinase inhibition activity of ultrasonic-extracted polysaccharides from longan fruit pericarp" 294-300 1-10 第110卷, 第2期 2 * |
| 《食品研究与开发》 20061005 王玲等 "龙眼多糖结构和性质的研究" 21-25 1-10 第27卷, 第10期 2 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104865333A (en) * | 2015-05-14 | 2015-08-26 | 武汉轻工大学 | Detection method of longan meat protein polysaccharide |
| CN104865333B (en) * | 2015-05-14 | 2016-08-24 | 武汉轻工大学 | A kind of detection method of Arillus Longan proteoglycan |
| CN107987177A (en) * | 2017-11-30 | 2018-05-04 | 广西壮族自治区农业科学院农产品加工研究所 | A kind of isolation and purification method of hypoglycemic Arillus longan polysaccharide |
| CN107987177B (en) * | 2017-11-30 | 2020-06-30 | 广西壮族自治区农业科学院农产品加工研究所 | Separation and purification method of polysaccharide of longan pulp for reducing blood sugar |
| CN108003255A (en) * | 2017-12-22 | 2018-05-08 | 茂名市水果科学研究所 | A kind of longan polysaccharide extracting method |
| CN108948220A (en) * | 2018-06-28 | 2018-12-07 | 广东省农业科学院蚕业与农产品加工研究所 | A kind of low viscosity, the preparation method of the prebiotic active polysaccharide of highly dissoluble longan |
| CN116606385A (en) * | 2023-04-28 | 2023-08-18 | 广州工程技术职业学院 | Zedoary turmeric polysaccharide and preparation method and application thereof |
| CN116606385B (en) * | 2023-04-28 | 2024-04-16 | 广州工程技术职业学院 | Zedoary turmeric polysaccharide and preparation method and application thereof |
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Effective date of registration: 20160222 Address after: 510610 Tianhe District, Guangzhou, Dongguan, Zhuang Zhuang Road, No. 1, No. 133, Patentee after: Sericulture & Agri-Food Research Institute, GAAS Patentee after: Guangdong Bosun Health Food Co., Ltd. Address before: 510610 Tianhe District, Guangzhou, Dongguan, Zhuang Zhuang Road, No. 1, No. 133, Patentee before: Research Institute of Agricultural Biological Technology of GAAS Patentee before: Sericulture & Agri-Food Research Institute, GAAS Patentee before: Guangdong Bosun Health Food R & D Center |