CN102068606B - Wrinkle-removing and anti-wrinkle Chinese medicinal composition, preparation and preparation method thereof - Google Patents
Wrinkle-removing and anti-wrinkle Chinese medicinal composition, preparation and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a wrinkle-removing and anti-wrinkle Chinese medicinal composition, which is mainly prepared from the following raw material medicaments in part by weight: 10 to 20 parts of astragalus, 10 to 30 parts of dwarf lilyturf tuber, 10 to 25 parts of heterophylly falsestarwort root, 10 to 20 parts of root of red-rooted salvia and 5 to 20 parts of liquoric root. Selected raw materials are natural soothing components, have low skin irritation, permeate into skin basal layer in various ways, recover skin vigor, clear free radicals and play a fundamental role in resisting and removing wrinkles. Experiments prove that the wrinkle-removing and anti-wrinkle Chinese medicinal composition has a remarkable wrinkle-removing effect, can remarkably improve skin texture after being used for 30 days, makes skin texture clear, can enhance skin glossiness and make the skin look fine, mellow and white. The wrinkle-removing and anti-wrinkle Chinese medicinal composition is prepared from readily-available raw materials by a convenient preparation method, has low cost, a very good effect and very good application prospect and market prospect, and can be easily accepted by customers.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition, specifically a kind of Chinese medicine composition with the crease-resistant effect of wrinkle removal, and by external preparation of said composition preparation and preparation method thereof.
Background technology
Wrinkle is the initial sign of skin aging.Wrinkle further develops, and can form pleat, i.e. darker pleat on the skin.After 25 years old, the ageing process of skin begins, and wrinkle occurs gradually.The order that occurs generally is forehead, goes up palpebra inferior, paropia, ear proparea, cheek, cervical region, following chin, mouthful week.The reason that causes wrinkle to produce is a lot, and from the angle of theory of Chinese medical science, the functional activity of the inherent internal organs of wrinkle and human body is closely related; From the angle of modern medicine, think that then the appearance of wrinkle is relevant with age, histrionic muscles and gravity.When histrionic muscles shrank, skin can shrink and wrinkle occur.Normally, young skin has certain elasticity and tension force, after histrionic muscles was lax, skin can very fast recovery, and wrinkle is disappeared.After the people entered the middle age, skin began obviously aging, and thinning of skin, hardening, drying, tension force reduce.Corium elastic fibrosis, fracture reduce the tension force of skin and elasticity, and like this, after histrionic muscles was lax, skin can not very fast recovery, and the wrinkle that then makes of a specified duration " solidifies ", and histrionic muscles not shrinkage mark still exists.Increase with the age, skin and subcutaneous tissue are more lax, add the atrophy or the disappearance of facial supporting tissue, and muscle is soft, and sliding bob will take place under the effect of gravity skin, forms darker wrinkle.
Wrinkle removal product in the market continues to bring out, and advertisement sentence sentence is tempting, but effect allows the people sigh.Many cosmetology skin care productss can only be absorbed by skin surface to a great extent, can not really enter skin corium, reach profound renewal and the purpose that collagen protein is replenished, and play the effect of removing the wrinkle slow down aging.Some cosmetics in order to reach " mystery " effect of quick wrinkle removal, adds hormones or other violated components in playing skin care item, though can reach the wrinkle removal effect fast, for skin health is buried serious hidden danger.
Therefore, working out a kind of skin care item that can desalinate, smooth away wrinkles and have long-acting anti-wrinkle effect is focuses that those skilled in the art study.The wrinkle removal skin care item of Chinese medicine adding ingredient are a lot of at present, shortcomings such as but Chinese medicine generally has the ion concentration height, the smell is awful, therefore, the Chinese medicine cosmetics that declare in market are for fear of these shortcomings, majority all is that Chinese medicine or a few flavor Chinese medicine simply add simply, or addition seldom, and crude drug is not according to traditional traditional Chinese medical science thought prescription like this, and the wrinkle removal anti-wrinkle effect is also not obvious.
Summary of the invention
The purpose of this invention is to provide a kind of Chinese medicine composition that is used to have the crease-resistant effect of wrinkle removal.The present invention is primary raw material with the Chinese medicine extract, various component reasonable combination, cooperate shared, really can deep enough dermal layer of the skin, remove free radical, fundamentally smooth away wrinkles, life-time service has good anti-wrinkle effect.
Another object of the present invention is to propose a kind of skin protection extract that utilizes the above-mentioned composition preparation.
A further object of the present invention is preparation that proposes above-mentioned skin protection extract preparation and preparation method thereof.
The present invention selects the Radix Astragali, Radix Ophiopogonis, Radix Pseudostellariae, Radix Salviae Miltiorrhizae and Radix Glycyrrhizae five tastes drug regimen for use, extracts active component by special process, this active component preparation is become skin care formulation, thereby effectively play the effect of wrinkle removal reduce wrinkle.The brief introduction of five tastes medicine medical material effect is as follows:
The Radix Astragali: contain sucrose, glucuronic acid, astragalus polysaccharides A, B, C, D, lymphatic temperament, several amino acids, bitter principle, Radix Astragali saponin, choline, folic acid, flavane chemical compound and contain various trace elements such as selenium, silicon, zinc, cobalt, copper, molybdenum.Nature and flavor: sweet, tepor.Return lung, spleen, liver, kidney channel.The Radix Astragali has benefiting QI for strengthening the superficies, arresting sweating to take off, hold in the palm the effect of skin ulcer granulation promoting, inducing diuresis to remove edema admittedly.It is weak to be used for the treatment of the deficiency of vital energy, sinking of QI of middle-JIAO, and the chronic diarrhea proctoptosis, the metrorrhagia of having blood in stool, the exterior deficiency spontaneous perspiration, carbuncle is difficult bursts, and burst for a long time and do not hold back, blood deficiency and yellow complexion, interior-heat is quenched one's thirst, chronic nephritis, albuminuria, diabetes etc.
Radix Ophiopogonis: have the effect of replenishing YIN and removing heat, nourishing the lung to arrest cough, be used for the treatment of the sick impairment of body fluid of interior-heat caused by deficiency of YIN or calentura, restlessness and thirst.Cough, expectorant due to nourishing the lung to arrest cough treatment, the dryness-heat impairing the lung is thick, and the effect of QI rising in reverse order etc.
Radix Pseudostellariae: the Radix Pseudostellariae root contains fructose, starch, Saponin.It is reported again, contain free amino acid, the highest with arginine, glutamic acid, aspartate content, account for 30%～40% of free amino acid, also contain maltose etc.Radix Pseudostellariae is mainly used in the lack of appetite asthenia of deficiency-weakness of spleen-QI, deficiency of stomach-Yin.Energy strengthening the spleen gas, nourishing stomach-YIN.Also be used for deficiency of the lung cough caused by dryness, cardiopalmus sleeplessness and the deficiency-heat hyperhidrosis etc. that hinder in deficiency of vital energy Tianjin.
Radix Salviae Miltiorrhizae: the nature and flavor hardship is slightly cold.GUIXIN, Liver Channel.Effect: promoting blood flow to regulate menstruation, stasis-dispelling and pain-killing, removing heat from blood eliminating carbuncle, the relieving restlessness that clears away heart-fire, nourishing blood to tranquillize the mind.Cure mainly: menoxenia, amenorrhea dysmenorrhea, lump in the abdomen, breast ventral spine pain, pyretic arthralgia pain, skin infection swell and ache, dysphoria and insomnia, hepatosplenomegaly and angina pectoris etc.
Radix Glycyrrhizae: Radix Glycyrrhizae property is flat, and sweet in the mouth is returned 12 warps.Detoxifcation arranged, eliminate the phlegm, pain relieving, spasmolytic be so that pharmacological action such as anticancer.On the traditional Chinese medical science, the Radix Glycyrrhizae invigorating the spleen and replenishing QI is grown and to be coughed lung moistening, and the emergency detoxifcation is in harmonious proportion hundred medicines.
The present inventor carries out compatibility with above-mentioned five tastes medicine, and wherein the Radix Astragali has the effect that tonifying Qi and lifting yang, benefit are defended consolidating superficial resistance, promoting pus discharge and tissue regeneration strengthening and diuretic detumescent.Radix Ophiopogonis can YIN nourishing and the production of body fluid promoting, the Radix Pseudostellariae tonifying QI to produce body fluid, and Radix Salviae Miltiorrhizae can blood circulation promoting and blood stasis dispelling and removing heat from blood.Radix Glycyrrhizae benefiting vital QI and blood coordinating the actions of various ingredients in a prescription.The inventor proposes the optimum amount of said composition external, makes each medicine produce cooperative effect, and external wrinkle removal anti-wrinkle effect is very remarkable, is natural plant wrinkle removal agent.
Concrete technical scheme of the present invention is as follows:
A kind of Chinese medicine composition with the crease-resistant effect of wrinkle removal, described compositions are mainly made by following raw materials in weight portion medicine:
10～20 parts of the Radixs Astragali, 10～30 parts of Radix Ophiopogonis, 10～25 parts of Radix Pseudostellariaes, 10～20 parts of Radix Salviae Miltiorrhizaes, 5～20 parts in Radix Glycyrrhizae.
The preferable weight portion proportioning of each crude drug of described compositions is:
15～20 parts of the Radixs Astragali, 10～25 parts of Radix Ophiopogonis, 15～20 parts of Radix Pseudostellariaes, 10～15 parts of Radix Salviae Miltiorrhizaes, 5～15 parts in Radix Glycyrrhizae.
Each crude drug optimum weight part proportioning of described compositions is:
17 parts of the Radixs Astragali, 13 parts of Radix Ophiopogonis, 15 parts of Radix Pseudostellariaes, 10 parts of Radix Salviae Miltiorrhizaes, 5 parts in Radix Glycyrrhizae.
A kind of method for preparing the above-mentioned Chinese medicine composition of right, concrete steps are as follows:
(1) takes by weighing each crude drug according to said components and consumption;
(2) crude drug is mixed add entry, boil from liquid and constantly pick up counting, boiled 30～180 minutes; The mass volume ratio g/ml of described crude drug and water is 0.5～1: 10～20;
(3) stop heating, be cooled to 30～50 ℃, filter;
(4) after the filtrate that obtains of step (3) is filtered once more,, promptly prepare activity extract through vacuum filtration.
Mass volume ratio g/ml the best of crude drug and water described in the described step (2) is 1: 10.
Filter in described step (3) and/or the step (4) is that 100～200 orders filter.
Vacuum filtration is 500～700 order fineness vacuum filtrations in the described step (4), and vacuum is 0.05～0.1MPa.
Described water can adopt deionized water, distilled water or pure water.
A kind of activity extract with the crease-resistant effect of wrinkle removal, described extract are to prepare through above-mentioned method.
The application of above-mentioned activity extract in the preparation skin care item.
A kind of skin care product additive with the crease-resistant effect of wrinkle removal, make by following raw materials in weight portion medicine:
95～100 parts of above-mentioned activity extracts, 8～12 parts of glycerol, 2～4 parts of trehaloses.
According to use approach difference, the present composition is made external preparation, as external facial cream, emulsion, cosmetic water, cleansing milk, spray or essence.But be not limited to above-mentioned dosage form, can be according to known method in the skin care item industrial circle, aforementioned pharmaceutical compositions is made various external preparation after by the known method degerming.
Preparation of the present invention, its usage and consumption are as follows:
Method: after clean of every day, smear preparation of the present invention on skin, each is once sooner or later.
Consumption: determine according to individual demand, smear preparation of the present invention in right amount and get final product.For the position that is prone to wrinkle, can smear repeatedly as canthus and decree stricture of vagina place, be as the criterion with whole coverings.
Beneficial effect of the present invention:
Raw material selection of the present invention is the natural composition of releiving, and is little to skin irritation, gos deep into the skin basal layer by number of ways, recovers skin vitality, knows free radical, fundamentally plays the effect of crease-resistant wrinkle removal.Experimental results show that preparation wrinkle removal effect of the present invention is remarkable, use the texture that can obviously improve skin after 30 days, make the texture exquisiteness, can increase the glossiness of skin simultaneously, make skin fine and smooth mellow and full pale.Raw material of the present invention obtains simply, effect was excellent when preparation method was convenient, with low cost, is easy to be accepted by consumers in general, has good application prospects and market prospect.
Foregoing invention content part of the present invention has been done detailed description to technical scheme of the present invention, and those skilled in the art can repeat the present invention and obtain described technique effect according to foregoing.Below by the specific embodiment the technology of the present invention content is further explained, so that the clearer understanding of those skilled in the art is not limitation of the present invention, any being equal to, replace or known change all belongs to protection domain of the present invention.
Description of drawings
Describe the specific embodiment of the present invention in detail below in conjunction with accompanying drawing:
Fig. 1 is that Chinese medicine extraction liquid of the present invention is to DPPH measured by esr technique figure;
Fig. 2 is that Chinese medicine extraction liquid of the present invention is to ultra-oxygen anion free radical scavenging action figure;
Fig. 3 is that Chinese medicine extract of the present invention is to hydroxyl radical free radical scavenging action figure.
The specific embodiment
The used medical material of present embodiment all can be bought from China Medicament Group Corp. and obtain, and meets 2005 editions standards of Pharmacopoeia of People's Republic of China.Other compositions are the conventional adjuvant of skin care item and cosmetic field, all can obtain by commercially available purchase.Glycerol and trehalose are known commercially available prod, available from the many fragrant bio tech ltds of Beijing China.Kieselguhr is available from chemical reagents corporation of traditional Chinese medicines group, model: chemical pure 91053-39.
The preparation of embodiment 1 activity extract of the present invention
(1) take by weighing each crude drug according to following component and consumption:
Radix Astragali 17g, Radix Ophiopogonis 13g, Radix Pseudostellariae 15g, Radix Salviae Miltiorrhizae 10g, Radix Glycyrrhizae 5g.
(2) crude drug mix is added deionized water, boil from liquid and constantly pick up counting, boiled 45 minutes; The mass volume ratio g/ml of described crude drug and deionized water is 1: 10, promptly adds the 600ml deionized water;
(3) stop heating, be cooled to 30 ℃, 200 orders filter;
(4) after filtrate 200 orders that obtain of step (3) filtered, through kieselguhr 500 order vacuum filtrations, vacuum was 0.05～0.1MPa again, and vacuum has unsteady according to the variation of fluid flow, as long as in this scope, finally prepare activity extract.
Measure pH value, solid content, electrical conductivity, measurement result is: pH value is 5.0～6.5, solid content be 10～13% and electrical conductivity be 700～1200 μ s/cm
2
The preparation of embodiment 2 activity extracts of the present invention
(1) take by weighing each crude drug according to following component and consumption:
Radix Astragali 10g, Radix Ophiopogonis 30g, Radix Pseudostellariae 10g, Radix Salviae Miltiorrhizae 20g, Radix Glycyrrhizae 10g.
(2) crude drug mix is added deionized water, boil from liquid and constantly pick up counting, boiled 30 minutes; The mass volume ratio g/ml of described crude drug and deionized water is 1: 20, promptly adds the 1600ml deionized water;
(3) stop heating, be cooled to 45 ℃, 100 orders filter;
(4) after filtrate 100 orders that obtain of step (3) filtered, through kieselguhr 600 order vacuum filtrations, vacuum was 0.05～0.1MPa again, and vacuum has unsteady according to the variation of fluid flow, as long as in this scope, finally prepare activity extract.
Measure pH value, solid content, electrical conductivity, measurement result is: pH value is 5.0～6.5, solid content be 10～13% and electrical conductivity be 700～1000 μ s/cm
2
The preparation of embodiment 3 activity extracts of the present invention
(1) take by weighing each crude drug according to following component and consumption:
Radix Astragali 20g, Radix Ophiopogonis 10g, Radix Pseudostellariae 25g, Radix Salviae Miltiorrhizae 15g, Radix Glycyrrhizae 20g.
(2) crude drug mix is added deionized water, boil from liquid and constantly pick up counting, boiled 90 minutes; The mass volume ratio g/ml of described crude drug and deionized water is 0.5: 10, promptly adds the 1800ml deionized water;
(3) stop heating, be cooled to 50 ℃, 200 orders filter;
(4) after filtrate 200 orders that obtain of step (3) filtered, through kieselguhr 700 order vacuum filtrations, vacuum was 0.05～0.1MPa again, and vacuum has unsteady according to the variation of fluid flow, as long as in this scope, finally prepare activity extract.
Measure pH value, solid content, electrical conductivity, measurement result is: pH value is 5.0～6.5, solid content be 10～13% and electrical conductivity be 900～1200 μ s/cm
295 ℃ are incubated 30 minutes afterwards, sterilization; Water-bath is cooled to room temperature, adds the 0.6%LGP liquid preservative, obtains product; This antiseptic full name is LIQUID GERMALL PLUS, available from the international special product company limited of ISP.Afterwards as required, can carry out subsequent operation, as stability, zest, safety etc.
The preparation of embodiment 4 activity extracts of the present invention
(1) take by weighing each crude drug according to following component and consumption:
Radix Astragali 20g, Radix Ophiopogonis 10g, Radix Pseudostellariae 25g, Radix Salviae Miltiorrhizae 15g, Radix Glycyrrhizae 20g.
(2) crude drug mix is added deionized water, boil from liquid and constantly pick up counting, boiled 60 minutes; The mass volume ratio g/ml of described crude drug and deionized water is 1: 15, promptly adds the 1350ml deionized water;
(3) stop heating, be cooled to 35 ℃, 100 orders filter;
(4) after filtrate 100 orders that obtain of step (3) filtered, through fine straining plate 500 order vacuum filtrations, vacuum was 0.05～0.1MPa again, and vacuum has unsteady according to the variation of fluid flow, as long as in this scope, promptly prepare activity extract.
(5) add glycerol and trehalose in the filtrate, activity extract: glycerol: the mass ratio of trehalose is 100: 10: 2.
Measure pH value, solid content, electrical conductivity, measurement result is: pH value is 5.0～6.5, solid content be 10～13% and electrical conductivity be 800～1000 μ s/cm
295 ℃ are incubated 30 minutes afterwards, sterilization; Water-bath is cooled to room temperature, adds the 0.6%LGP liquid preservative, obtains product; This antiseptic full name is LIQUID GERMALL PLUS, available from the international special product company limited of ISP.Afterwards as required, can carry out subsequent operation, as stability, zest, safety etc.
The preparation of embodiment 5 activity extracts of the present invention
(1) take by weighing each crude drug according to following component and consumption:
Radix Astragali 15g, Radix Ophiopogonis 20g, Radix Pseudostellariae 20g, Radix Salviae Miltiorrhizae 10g, Radix Glycyrrhizae 15g.
(2) crude drug mix is added deionized water, boil from liquid and constantly pick up counting, boiled 180 minutes; The mass volume ratio g/ml of described crude drug and deionized water is 0.5: 15, promptly adds the 2400ml deionized water;
(3) stop heating, be cooled to 40 ℃, 200 orders filter;
(4) after filtrate 200 orders that obtain of step (3) filtered, through fine straining plate 600 order vacuum filtrations, vacuum was 0.05～0.1MPa again, and vacuum has unsteady according to the variation of fluid flow, as long as in this scope, promptly prepare activity extract.
(5) add glycerol and trehalose in the filtrate, activity extract: glycerol: the mass ratio of trehalose is 95: 8: 4.
Measure pH value, solid content, electrical conductivity, measurement result is: pH value is 5.0～6.5, solid content be 10～12% and electrical conductivity be 800～1200 μ s/cm
295 ℃ are incubated 30 minutes afterwards, sterilization; Water-bath is cooled to room temperature, adds the 0.6%LGP liquid preservative, obtains product; This antiseptic full name is LIQUIDGERMALL PLUS, available from the international special product company limited of ISP.Afterwards as required, can carry out subsequent operation, as stability, zest, safety etc.
The preparation of embodiment 6 facial creams of the present invention
Component A: Cera Flava 3g, lanoline 4g, stearic acid 2g, white oil 6g, vitamin E 8g
Activity extract 25g, distilled water 46.4, Margarita powder 2g, the chitosan 3g of B component: embodiment 5 preparations;
Component C: vitamin C 0.15g, essence 0.25g, antiseptic 0.2g
Concrete preparation method: component A is mixed and heated to 75 ℃; The B group is mixed and heated to 75 ℃; Under 45 rev/mins of mixing speeds, component A is joined in the B component evenly, carry out emulsifying.Add the C group when cooling the temperature to 50 ℃, stir, finished product is promptly made in packing.
The preparation of the crease-resistant emulsion of embodiment 7 wrinkle removal of the present invention
1, raw material and consumption:
A phase: cetearyl alcohol 5wt%
The two stearate 2wt% of tetramethylolmethane
Glyceryl monostearate 2wt%
Squalane 2wt%
Shea butter 5wt%
Dimethicone 3wt%
Bisabolol 0.5wt%
B phase: spermaceti hard base glucoside (emulsifying agent) 3wt%
HANSHENGJIAO 0.05wt%
Amino acid humectant 1wt%
Disodium EDTA (chelating agen) 0.15wt%
α-mannan 6wt%
Glycerol 4wt%
Butanediol 2wt%
Embodiment 2 preparation Chinese medicine extract 15wt%
C phase: antiseptic 0.5wt%
Essence 0.2wt%
10％NaOH 0.5wt％
Surplus is a pure water.
2, preparation method:
(1) takes by weighing each raw material by above-mentioned component and consumption;
(2) A being heated to 80～85 ℃ of stirrings respectively mutually with B mutually makes material dissolution complete;
(3) A is added in the emulsifying pot stirs, stir speed (S.S.) is 80 rev/mins, B is added in the emulsifying pot while stirring, the speed that adds is as the criterion with the whole emulsifyings of B phase raw material in the emulsifying pot, evacuation after B phase raw material all adds (vacuum is-0.1 atmospheric pressure), stirring homogenizing 5 minutes, stir speed (S.S.) is 80 rev/mins, be heated to 80 ℃ of insulations, begin cooling after 20 minutes, when dropping to 30 ℃, temperature adds C phase raw material, stir and continue cooling, when temperature during at 40 ℃ homogenizing discharging in 2 minutes get final product.
The activity extract efficacy test
1, remove DPPH free radical ability and measure, the extract of selecting embodiment 1 preparation for use is as sample, and this test method is carried out with reference to the method that following document provides:
Wang Chen, Zhang Jinmu. Study on Antioxidant Activity of Extracts of Celery Leaf [J]. Anhui agricultural sciences, 2006,34 (13): 3174-3175,3177.
LARRAURI?J?A，SNCHEZMORENO?C，SAURACALLIXTO.Effect?of?temperature?on?the?free?radical?scavenging?capacity?of?extracts?from?red?and?white?grape?peels[J].Agric?Food?Chem，1998，46：2694.
DPPH be a kind of very stable be the free radical at center with nitrogen, if extract can be removed it, then point out extract to have to reduce the valid density of hydroxy radical, alkane free radical or oxyradical and interrupt the effect (Wang Chen etc., 2006) of lipid peroxidation chain reaction.DPPH is highly stable in organic solvent, is purple, and a characteristic absorption peak is arranged at the 517nm place, and when it ran into free radical scavenger, the lone electron of DPPH was matched, and its color is shoaled, and diminishes at the absorbance of maximum absorption wave strong point.Therefore, can estimate removing effect by the variation of measuring absorbance to DPPH.
According to Larrauri (1998) method, accurately take by weighing 20mgDPPH, in the 250ml volumetric flask, obtaining concentration is the DPPH solution of 20mmol/L, the extract sample is become the test fluid of variable concentrations respectively with distilled water diluting with the dehydrated alcohol standardize solution.Get the 20mmol/L DPPH mixing afterreaction 30min of 2ml test fluid and 2ml, measure the variation of absorbance under the wavelength 517nm, control solvent replaces with dehydrated alcohol, is calculated as follows the suppression ratio of antioxidant.
Suppression ratio (%)=[1-(A
i-A
i)/Ac] * 100
In the formula, A
iAbsorbance for 2mlDPPH liquid and 2ml extracting solution; A
jAbsorbance for 2ml extracting solution and 2ml dehydrated alcohol; Ac is the absorbance of 2mlDPPH liquid and 2ml dehydrated alcohol.
Remove the experimental result of DPPH free radical ability mensuration and see Fig. 1, as can be seen from Figure 1, the concentration of extract is big more, and is strong more to the removing ability of DPPH free radical, good removing effect is just arranged when extract concentration reaches 3%, and this shows that extracting solution has the ability of good removing DPPH.
2, remove the superoxide anion ability and measure, the extract of selecting embodiment 4 preparations for use is as sample.
This test method is carried out according to the method that following document provides: model dawn, Yan Xiaojun, Fang Guoming etc.High molecular Brown algae polyphenol antioxidant properties research [J]. aquatile journal, 1999,23 (5): 494-499.
Get the Tris-HCl buffer 4.5ml of 0.05mol/L pH8.2, place 25 ℃ of water-bath preheating 20min, the sample and the 0.4ml that add the 1ml variable concentrations respectively, the pyrogallol solution of 25mmol/L, in 25 ℃ of water-baths, react 5min behind the mixing, add 8mol/L HCl 1.0ml cessation reaction, make reference with the Tris-HCl buffer, measure absorbance at the 299nm place, calculate clearance rate.The blank group replaces sample with 1ml sample solvent, and each is handled and all does three repetitions.
The computing formula of clearance rate: ultra-oxygen anion free radical clearance rate (%)=100 (A
1-A
2)/A
1, A wherein
1Be barren mean light absorbency, A
2Mean light absorbency for sample.
Extracting solution to ultra-oxygen anion free radical scavenging action see Fig. 2, as can be seen from Figure 2, extract concentration is big more, and is strong more to the removing ability of superoxide anion.100% stock solution can keep 92.56% clearance rate, proves that extracting solution provided by the invention has the ability of stronger removing ultra-oxygen anion free radical, can be used for preparing cosmetics, especially is used for preparing the cosmetics with anti-ageing effect.
3, to the mensuration (salicylic acid method) of Hydroxyl Radical Scavenging, the extract of selecting embodiment 5 preparations for use is as sample.
This test method is carried out according to the method that following document provides: in Ding Lijun, all ditch between fields brightness, Lin Yanru, the Dicranopteris dichotoma; The extraction of flavonoid substance and Study on antioxidation thereof [J]. Food Science, 2005,26 (8): 77-83.
Hydroxyl radical free radical is produced by the Fenton reaction, and OH oxidation salicylic acid produces has 2 of characteristic absorption to 510nm light, and the 3-resorcylic acid is caught the clearance rate that the resulting product of OH is determined OH by measuring salicylic acid.Hydroxyl radical free radical is the most active free radical of chemical property in the active oxygen, and it almost can react with any biomacromolecule in the living cells, and response speed is exceedingly fast, and is to the maximum free radical of body harm.Catch the clearance rate that the resulting product of OH is determined OH by measuring salicylic acid, can reduce this light absorption value if add material in the reaction system with removing OH effect.Therefore can the amount of hydroxyl and the ability that test substance is removed hydroxyl radical free radical be described by its content of spectrophotometry.
2mmol/L FeSO in the 25ml color comparison tube
43ml, 1mmol/L H
2O
23ml shakes up, and then adds 6mmol/L salicylic acid 3ml, shakes up, and takes out behind 37 ℃ of heating in water bath 15min, surveys its absorbance A 0.Add certain density liquid 0.2ml to be measured, 0.4ml, 0.6ml, 0.8ml, 1.0ml then respectively, and then add distilled water 0.8ml, 0.6ml, 0.4ml, 0.2ml, 0ml respectively, shake up, continue heating in water bath 15min, take out and survey its absorbance A x.The reduction of the system absorbance that is caused for common 1.0ml liquid and the distilled water to be measured that adds after eliminating, method is the same, surveys its absorbance A behind the constant temperature 15min
00, add the 1ml distilled water, survey once its absorbance A after shaking up again
Xx, A
Reduce=A
00-A
Xx
Liquid to be measured to the OH clearance rate is: OH clearance rate (%)=100 (A
0-Ax-A
Reduce)/A
0
Measurement result to Hydroxyl Radical Scavenging is seen Fig. 3, and as can be seen from Figure 3, extract concentration is big more, and is strong more to the removing ability of hydroxy radical.Proof the present invention has good effect to knowing free radical, and wrinkle removal is crease-resistant effectively.
Clinical experiment of the present invention and result
Experimental technique: select age bracket at 25 years old to 55 years old volunteer, 5030 of male volunteers, 5030 of women volunteers.Cardinal symptom: face has microgroove, some position wrinkle is obvious, the colour of skin is obscure.Volunteer is divided into two or three group at random, every group 2050 people.
Test group: group 1: after clean of every day, the anti-wrinkle face cream of smearing the embodiment of the invention 6 preparations on facial skin is an amount of, and each is once sooner or later.Group 2: after clean of every day, the wrinkle removal emulsion of smearing the embodiment of the invention 7 preparations on facial skin is an amount of, and each is once sooner or later.Group 3 (matched groups): after clean of every day, it is an amount of to smear commercially available wrinkle removal emulsion on facial skin, and each is once sooner or later.3 days, 7 days, 1 month, 3 months each statistics once.
Evaluation criterion:, do skin texture experiment and naked eyes visual experiment by patient's readme impression.Effectively: the skin texture degree obviously attenuates greasy, and perusal face microgroove obviously reduces, and dark stricture of vagina shoals.Invalid: skin texture and the preceding no any variation of experiment.
Result on trial: 3 days groups 1 and organize 2 experimenters and have the wrinkle removal wrinkle removal effect that obviously is better than the commercially available prod, group 1 and organize 2 effective percentage and all be higher than 80%, the commercially available prod only is 50%.Use after 30 days group 1 and organize 2 effective percentage to reach 100%, the commercially available prod effective percentage is 80%.Group 1 and organize the glossiness that 2 products all can obviously increase skin, the skin exquisiteness is mellow and full, also has certain whitening effect.There is not an example reaction phenomenons such as allergy, skin discomfort to occur.
Claims (10)
1. the Chinese medicine composition with the crease-resistant effect of wrinkle removal is characterized in that, described compositions is made by following raw materials in weight portion medicine:
10～20 parts of the Radixs Astragali, 10～30 parts of Radix Ophiopogonis, 10～25 parts of Radix Pseudostellariaes, 10～20 parts of Radix Salviae Miltiorrhizaes, 5～20 parts in Radix Glycyrrhizae.
2. Chinese medicine composition according to claim 1 is characterized in that, described compositions is made by following raw materials in weight portion medicine:
15～20 parts of the Radixs Astragali, 10～25 parts of Radix Ophiopogonis, 15～20 parts of Radix Pseudostellariaes, 10～15 parts of Radix Salviae Miltiorrhizaes, 5～15 parts in Radix Glycyrrhizae.
3. Chinese medicine composition according to claim 2 is characterized in that, described compositions is made by following raw materials in weight portion medicine:
17 parts of the Radixs Astragali, 13 parts of Radix Ophiopogonis, 15 parts of Radix Pseudostellariaes, 10 parts of Radix Salviae Miltiorrhizaes, 5 parts in Radix Glycyrrhizae.
4. method for preparing claim 1,2 or 3 described Chinese medicine compositions is characterized in that concrete steps are as follows:
(1) takes by weighing each crude drug according to claim 1,2 or 3 described components and consumption;
(2) crude drug is mixed add entry, boil from liquid and constantly pick up counting, boiled 30～180 minutes; The mass volume ratio g/ml of described crude drug and water is 0.5～1: 10～20;
(3) stop heating, be cooled to 30～50 ℃, filter;
(4) after the filtrate that obtains of step (3) is filtered once more,, promptly prepare activity extract through vacuum filtration.
5. method according to claim 4 is characterized in that, filtering in described step (3) and/or the step (4) is that 100～200 orders filter.
6. method according to claim 5 is characterized in that, the mass volume ratio g/ml of crude drug and water is 1: 10 described in the described step (2).
7. method according to claim 6 is characterized in that, vacuum filtration is 500～700 order fineness vacuum filtrations in the described step (4), and vacuum is 0.05～0.1MPa.
8. the activity extract with the crease-resistant effect of wrinkle removal is characterized in that, described extract prepares through claim 5,6 or 7 described methods.
9. the application of the described activity extract of claim 8 in the preparation skin care formulation.
10. the skin care product additive with the crease-resistant effect of wrinkle removal is characterized in that, is made by following raw materials in weight portion medicine:
95～100 parts of the described activity extracts of claim 8,8～12 parts of glycerol, 2～4 parts of trehaloses.
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| CN102302426B (en) * | 2011-09-09 | 2012-12-26 | 烟台新时代健康产业日化有限公司 | Skin-caring composition with speckle fading away and wrinkle removing effects |
| CN103142962B (en) * | 2013-03-20 | 2015-04-29 | 集粹坊科贸（北京）有限责任公司 | Foot protection composition, foot mask and preparation method of foot protection composition |
| CN103330736A (en) * | 2013-06-21 | 2013-10-02 | 冯雪群 | Skin whitening and blood nourishing health care lotion |
| CN105193649B (en) * | 2015-05-28 | 2018-04-10 | 孟宏 | With radiation proof and repair the external skin-care composition of skin barrier effect, toner and preparation method thereof |
| CN105434839B (en) * | 2015-05-28 | 2019-02-05 | 孟宏 | Have effects that radiation protection and repair the external skin-care composition of skin barrier, gel and preparation method thereof |
| CN105194286B (en) * | 2015-05-28 | 2019-03-01 | 孟宏 | Have effects that radiation protection and repair the external skin-care composition of skin barrier, essence and preparation method thereof |
| CN105434212B (en) * | 2015-05-28 | 2018-06-12 | 孟宏 | There is radiation protection and repair the external skin-care composition of skin barrier, lotion and preparation method thereof |
| CN105194285B (en) * | 2015-05-28 | 2019-02-05 | 孟宏 | Have effects that radiation protection and repair the external medicine composition of skin barrier, cream and preparation method thereof |
| CN107349126A (en) * | 2017-06-22 | 2017-11-17 | 芜湖福民生物药业股份有限公司 | Phosphocholine modified anti-wrinkle frost and preparation method thereof |
| CN109394850B (en) * | 2018-12-17 | 2021-06-29 | 温州古木生物科技有限公司 | Traditional Chinese medicine composition with skin aging delaying effect, aqueous extract and fermentation product of traditional Chinese medicine composition and application of traditional Chinese medicine composition |
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| CN101843572A (en) * | 2010-06-11 | 2010-09-29 | 成佩秀 | Skin-care anti-wrinkle face mask |
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