CN109394850B

CN109394850B - Traditional Chinese medicine composition with skin aging delaying effect, aqueous extract and fermentation product of traditional Chinese medicine composition and application of traditional Chinese medicine composition

Info

Publication number: CN109394850B
Application number: CN201811551622.8A
Authority: CN
Inventors: 陈克; 陈万胜
Original assignee: Wenzhou Gumu Biotechnology Co ltd
Current assignee: Wenzhou Gumu Biotechnology Co ltd
Priority date: 2018-12-17
Filing date: 2018-12-17
Publication date: 2021-06-29
Anticipated expiration: 2038-12-17
Also published as: CN109394850A

Abstract

The invention relates to the technical field of traditional Chinese medicine compositions for delaying skin aging, and particularly relates to a traditional Chinese medicine composition with an effect of delaying skin aging, an aqueous extract and a fermentation product thereof, and an application thereof. In order to achieve the purpose of delaying skin aging, the invention takes the traditional Chinese medicine theory of China as guidance, Chinese medicine raw materials are screened through a large number of long-term experiments, codonopsis pilosula, radix pseudostellariae, gynostemma pentaphylla, liquorice and moutan bark are effectively combined together, and extracts prepared by water extraction and leavening prepared by yeast fermentation of the composition can directly act on skin, have no toxic or side effect, and are simple and safe to use and mild in action. The skin care product has the effects of repairing exogenous aging and damage of skin, promoting the growth of skin cells, removing free radicals and resisting oxidation, can reduce the generation of wrinkles, and has the obvious effect of delaying skin aging when being applied to cosmetics.

Description

Traditional Chinese medicine composition with skin aging delaying effect, aqueous extract and fermentation product of traditional Chinese medicine composition and application of traditional Chinese medicine composition

Technical Field

The invention relates to the technical field of traditional Chinese medicine compositions for delaying skin aging, and particularly relates to a traditional Chinese medicine composition with an effect of delaying skin aging, an aqueous extract and a fermentation product thereof, and an application thereof.

Background

Aging is an inevitable physiological phenomenon, and skin aging as a part of the whole aging of the body has prominent psychological and social significance, because the aging of the body is most clearly and intuitively expressed on the skin, and the change of the characteristic state of the skin is also used as an important mark for judging the age of a person. Skin aging is classified into natural aging and extrinsic aging. Natural aging is related to factors such as age and heredity of human body, and is an irreversible natural law. Exogenous aging refers to skin aging caused by damage, degradation, denaturation, etc. of various cell components and structures of the skin due to external factors such as sunlight ultraviolet radiation, smoking, drinking, climate, air pollution, and exposure to harmful chemicals.

The most characteristic change of skin aging is the change of the dermal matrix components, which include all intercellular substances in the dermis except water, wherein the most important components are elastic fibers, collagen fibers, aminopolysaccharides, proteoglycans and the like, which play an important role in the elasticity and compliance of the skin. During the aging process of the skin, various factors cause the elastic fibers and collagen fibers to be degenerated, degraded and disappeared, and the elasticity and the compliance of the skin are lost, and changes such as wrinkles, slackness, atrophy, thinning, keratinization, pigmentation, telangiectasia and the like appear, wherein the wrinkles are the most basic clinical manifestations of the skin aging.

In recent years, with the social development of China and the improvement of the living standard of people, the demand of consumers for products for delaying skin aging is increased rapidly, but at present, certain toxic and side effects exist in the anti-aging process of oral medicines and health care products, and certain infection risks also exist in the improvement of the appearance of skin through invasive medical plastic of human bodies such as subcutaneous injection, surgical operation and the like.

Therefore, how to safely, effectively and simply obtain the effective components of natural plants aiming at delaying skin aging and apply the effective components to cosmetics is a problem to be solved urgently at present.

Disclosure of Invention

In order to solve the problems of the prior art, the invention provides a traditional Chinese medicine composition with the effect of delaying skin aging, an aqueous extract, a fermentation product and application thereof. Has the functions of repairing exogenous aging and damage of skin, promoting the growth of skin cells, eliminating free radicals and resisting oxidation, and can reduce the generation of wrinkles so as to achieve the effect of delaying skin aging.

The technical scheme adopted by the invention is as follows:

a traditional Chinese medicine composition with a skin aging delaying effect comprises the following components in parts by mass: 200-350 parts of codonopsis pilosula, 200-350 parts of radix pseudostellariae, 100-200 parts of gynostemma pentaphylla, 100-200 parts of liquorice and 100-200 parts of moutan bark.

An aqueous extract of a traditional Chinese medicine composition is prepared by adding 10 times of pure water by weight into the traditional Chinese medicine composition, extracting for 1 hour in a vacuum homogenizing emulsifying pot at 95-100 ℃, and filtering to obtain a filtrate.

A fermented product of Chinese medicinal composition is prepared by mixing the Chinese medicinal composition and zymocyte to obtain initial system, adding 10 times of pure water, fermenting to obtain fermentation liquid, sterilizing, and filtering to obtain filtrate.

The zymocyte is saccharomycete, and the high-activity dry yeast is added into the traditional Chinese medicine composition according to 100-350 parts by weight.

In the fermentation step, the temperature is 25-45 ℃; the stirring speed is 0-50 rpm; the time is 4-24 h.

An application of the aqueous extract of a traditional Chinese medicine composition is that the aqueous extract is mixed into cosmetics according to the proportion of 1 to 60 percent by weight.

The application of fermented product of Chinese medicine composition is that the fermented product is mixed into cosmetics in the weight ratio of 1-60%.

The range of cosmetics used for aqueous extracts or fermentations is: liquid, emulsion, cream, jelly, powder, or powder molding.

Codonopsis pilosula, according to 282 pages of the first part of the 2015 edition of "Chinese pharmacopoeia" edited by the national pharmacopoeia committee of 1 st edition of 6 months in 2015 of Chinese pharmaceutical science and technology publishing house, the following effects are recorded: to invigorate the spleen, benefit the lung, nourish blood and promote the production of body fluid. Can be used for treating deficiency of qi of spleen and lung, deficiency of qi and blood, and sallow complexion. According to the records of the Shanghai science and technology Press 2014, 6 th edition, 2 nd edition, edited by Nanjing university of traditional Chinese medicine, page 3836, radix Codonopsis has the effect of delaying aging, and the 20% radix Codonopsis water decoction can prolong the larval stage and the whole life cycle of silkworms and increase the weight after soaking the mulberry leaves in the silkworm. In vitro tests show that the radix Codonopsis extract can improve the activity of superoxide dismutase (SOD) in human blood, enhance the ability of scavenging free radicals, and prompt that radix Codonopsis has certain antiaging effect. The codonopsis pilosula has the function of enhancing the immunity of organisms, and the codonopsis pilosula injection can increase the number of macrophages in abdominal cavities of mice, increase the cell volume, increase pseudo feet and enhance the phagocytosis; the activities of DNA, RNA, saccharides, acid phosphatase (ACP), ATP enzyme, acid alpha-naphthol acetate esterase (ANAE) and succinate dehydrogenase in cells are obviously enhanced.

Radix pseudostellariae has the following effects according to the 68 pages in the first part of the 2015 edition of the Chinese pharmacopoeia (national pharmacopoeia) which is edited by the national pharmacopoeia committee of the 1 st edition of 6 months in 2015 of the Chinese medical science and technology publishing company: to replenish qi, invigorate the spleen, promote the production of body fluid and moisten the lung. Can be used for treating spleen deficiency, asthenia, and deficiency of both qi and yin. According to the records on page 463 of traditional Chinese medicine dictionary, which is edited by Nanjing university of traditional Chinese medicine in 6 th edition, 2014, Shanghai science and technology publishing company, the radix pseudostellariae decoction prolongs the average life and the maximum life of fruit flies, and has the effect of delaying aging.

The gynostemma pentaphylla has the following effects according to the records on pages 2133 and 2134 of the traditional Chinese medicine major dictionary compiled by Nanjing traditional Chinese medicine university of 6 months and 2 nd edition in Shanghai science and technology Press 2014: clear heat, tonify deficiency, remove toxicity. It can be used for treating asthenia, and leukopenia. And has the following functions: immunoregulation effect: gypenoside GPs (50 mg/kg and 100mg/kg) have bidirectional immunoregulation effect on normal mice. GPs can enhance the proliferation reaction of mouse splenocytes to ConA, PHA and LPS, enhance T cells in mixed lymphocytes, and promote IL-2 secretion of mouse splenocytes and IL-1 production of rat peritoneal macrophages. The function of delaying senility: the gynostemma pentaphylla can obviously prolong the passage generation of cell culture. Human skin cells were cultured in vitro, and the addition of GPs at 200. mu.g/ml of culture medium allowed the cells to pass 27 passages, whereas the control group only passed 22 passages. Similar results were obtained with human fetal lung diploid fibroblast subcultures, with the control group reaching 51 passages and the GPs group reaching 59 passages. 5-month-old mice are fed with herba Gynostemmatis decoction containing feed (2.5g basic feed containing 0.1g crude drug) for 4 months, and the SOD activity of the mice can be increased by 50% and 0% in control group after 2 months. The subacute aging model of the mice induced by the d-galactose is like that 15mg of gynostemma pentaphylla extract suspension is injected into the abdominal cavity of each mouse every day for 40 days, so that the decline of learning active avoidance reaction capability, the abnormal increase of the activity of monoamine oxidase (MAO-B) in the brain and the increase of lipofuscin of the brain of the mice of the aging model can be remarkably resisted, the atrophied thymus of the mice of the aging model is recovered to a normal level, and the enlarged spleen is also recovered to a normal level. Antioxidation: GPs reduce the amount of superoxide anions and hydrogen peroxide in human neutrophil phagocytes, reduce the excitation of chemiluminescence oxidation of human monocytes and murine macrophages, which is initiated by zymosan, and have a significant anti-oxidation effect. GPs also have improving and protecting effects on the decrease of vasodilation function caused by oxygen free radicals and the damage of vascular endothelial cells.

Licorice root, according to the Chinese pharmacopoeia 2015, published by the national pharmacopoeia committee of 1 st edition of 6 months in 2015, the first 87 pages of which have the following effects: tonify spleen and qi, clear heat and remove toxicity, relieve spasm and pain, and harmonize the drugs. Can be used for treating carbuncle, swelling and sore, and relieving drug toxicity and strong property. According to the record of the Shanghai science and technology Press 2014, 6 th edition, 2 nd edition, edited by Nanjing university of traditional Chinese medicine, page 685 of Chinese medicine, the licorice leachate externally inhibits Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, streptococcus B and the like, and the licorice hot water extract has insecticidal effect on clonorchis sinensis and antimicrobial effect. The freeze-dried water extract of Glycyrrhiza glabra L.in the case of intragastric administration of rats inhibits the function of adrenal-pituitary axis and promotes the production of rennin, and the Glycyrrhiza glabra L.preparation has adrenocorticoid-like effect. According to the records of the traditional Chinese medicine cosmetic science 38 edited by Liuhua Steel, version 1 of 11/2005 of Chinese medicine Press, licorice has the functions of resisting allergic reaction and detoxifying, can eliminate inflammation and toxic stimulation of skin, neutralize, relieve or reduce toxic substances of cosmetics, and can also prevent the allergic reaction of some cosmetics to some people.

Moutan bark, according to the first 172 pages of "chinese pharmacopoeia" 2015 edition, published by the national pharmacopoeia committee of 6 months 1 edition 2015 of chinese medical science and technology publishing house, has the following effects: clear heat and cool blood, activate blood and resolve stasis. It can be used for treating heat entering nutrient-blood, macula due to warm toxicity, carbuncle, swelling, and sore. According to records on pages 1367-1368 of a traditional Chinese medicine dictionary, which is compiled by Nanjing traditional Chinese medicine university at 6.2 edition of Shanghai science and technology Press, 25, 50 and 100mg/kg of cortex moutan total glycosides can obviously inhibit acute paw swelling of rats induced by carrageenan and ear edema of mice induced by xylene, and have an obvious dose-dependent relationship, the cortex moutan total glycosides (20-100 mg/kg) given 1 hour before inflammation has an obvious inhibiting effect on primary inflammation of rats with adjuvant arthritis, and the cortex moutan total glycosides (25-100 mg/kg) starts to be perfused 12 days after inflammation, and can obviously inhibit secondary inflammatory reaction of rats with adjuvant arthritis for 11 days continuously. 0.5-1 g/kg of paeonol is perfused into the stomach or 50-200 mg/kg of paeonol is injected into the abdominal cavity, and the paeonol has obvious inhibiting effect on carrageenan, egg white, formaldehyde, histamine, 5-hydroxytryptamine and bradykinin rat foot edema, dimethylbenzene mouse pinnacle swelling and the increase of permeability of abdominal cavity capillary vessels caused by acetic acid and endotoxin. Applying 5% cortex moutan powder cream (white yellowish cream prepared from cortex moutan) on topical skin injury. 27 cases of local acute eczema, 10 cases of seborrheic dermatitis, 2 cases of contact dermatitis and 1 case of neurodermatitis in clinical treatment; the course of the disease is 3 days to 3 years, and most of the disease is less than 1 month. As a result: 27 cases of acute eczema are taken for 2-20 days, 8 cases are cured, 5 cases have obvious effect, 6 cases are improved, and 8 cases are invalid; the 10 cases of seborrheic dermatitis are taken for 5-14 days, 2 cases are cured, 6 cases are improved, and 2 cases are invalid; 2 cases of contact dermatitis are taken for 3-5 days, 1 case is healed, and 1 case is ineffective; 1 example of neurodermatitis was not effective in half a month. In 4 effective cases, the rash recurred several days after drug withdrawal, and in 2 other cases, the drug withdrawal is caused by flushing, itching and burning.

The names of the used Cosmetic raw materials of the traditional Chinese medicine components in INCI (International Nomenclature for Cosmetic raw materials) are respectively as follows: radix Codonopsis (Codonopsis PILOSULA) extract, radix Pseudostellariae (Pseudostellaria HETEROPHYLLA) extract, herba Gynostemmatis (Gynostemma Pentaphyllum) extract, Glycyrrhrizae radix (Glycyrrhiza URALENSIS) root extract, and peony (Paeonia SUFFRUTICOSA) root bark extract.

The technical scheme provided by the invention has the beneficial effects that:

in order to achieve the purpose of delaying skin aging, the invention takes the traditional Chinese medicine theory of China as guidance, Chinese medicine raw materials are screened through a large number of long-term experiments, codonopsis pilosula, radix pseudostellariae, gynostemma pentaphylla, liquorice and moutan bark are effectively combined together, and extracts prepared by water extraction and leavening prepared by yeast fermentation of the composition can directly act on skin, have no toxic or side effect, and are simple and safe to use and mild in action. The skin care product has the effects of repairing exogenous aging and damage of skin, promoting the growth of skin cells, removing free radicals and resisting oxidation, can reduce the generation of wrinkles, and has the obvious effect of delaying skin aging when being applied to cosmetics.

The aqueous extract of the traditional Chinese medicine composition is prepared by adding 10 times of pure water by weight into the traditional Chinese medicine composition, extracting for 1 hour in a vacuum homogenizing emulsifying pot at 95-100 ℃, and filtering to obtain filtrate; the traditional Chinese medicine fermentation product is an initial system obtained by mixing the traditional Chinese medicine composition and zymocyte, 10 times of pure water by weight is added, and fermentation liquor obtained by fermentation is sterilized and filtered to obtain filtrate. Has mild and non-irritant action, safety and effectiveness, can be used for a long time, has simple process when being added into cosmetics, and is suitable for industrial production.

The aqueous extract and the fermentation product of the traditional Chinese medicine composition have wide application range, are well compatible with most of cosmetic raw materials, can be applied to various large dosage forms of cosmetics, and are simple and convenient to add.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the following detailed description is further provided in conjunction with the embodiments.

Example 1: is the application of the aqueous extract of the traditional Chinese medicine composition in the repair essence.

Preparing an aqueous extract of the traditional Chinese medicine composition: weighing 200g of codonopsis pilosula, 200g of radix pseudostellariae, 100g of gynostemma pentaphyllum, 100g of liquorice and 100g of moutan bark, cleaning, packaging with a sterilized gauze bag, putting into a vacuum homogenizing emulsifying pot, adding 10 times of pure water according to the weight of the traditional Chinese medicine composition, extracting for 1 hour at 95-100 ℃, and filtering to obtain medicinal juice, thus obtaining the aqueous extract of the traditional Chinese medicine composition.

The formula of the aqueous extract repair essence containing 20 wt% of the traditional Chinese medicine composition is shown in table 1:

TABLE 1

The components of the formulation of table 1 were prepared according to the following process:

(1) firstly, weighing all the components according to the component names and the weight in the table and putting the components into a stirring pot;

(2) mixing and stirring the 8 components in the phase A until the components are completely dissolved;

(3) stirring and adding the phase B until all the components are uniformly mixed and completely dissolved, and discharging.

Example 2: is the application of the water extract of the traditional Chinese medicine composition in the repair cream.

Preparing an aqueous extract of the traditional Chinese medicine composition: weighing 350g of codonopsis pilosula, 350g of radix pseudostellariae, 200g of gynostemma pentaphyllum, 200g of liquorice and 200g of moutan bark, cleaning, packaging with a sterilized gauze bag, putting into a vacuum homogenizing emulsifying pot, adding 10 times of pure water according to the weight of the traditional Chinese medicine composition, extracting for 1 hour at 95-100 ℃, and filtering to obtain medicinal juice, thus obtaining the aqueous extract of the traditional Chinese medicine composition.

The formula of the aqueous extract repair cream containing 15 wt% of the traditional Chinese medicine composition is shown in table 2:

TABLE 2

The components of the formulation of table 2 were prepared according to the following process:

(1) firstly, weighing each component according to the component names and the weight in the table;

(2) mixing the phase A components, heating to 82 ℃, and uniformly stirring;

(3) mixing and stirring the components of the phase B until the components are completely dissolved, and heating to 82 ℃;

(4) adding phase B into phase A, homogenizing in vacuum emulsifying pot at 3000rmp for 5min, stirring, cooling to 40 deg.C, and discharging.

Example 3: is the application of the traditional Chinese medicine composition in repairing emulsion.

Preparing an aqueous extract of the traditional Chinese medicine composition: weighing 300g of codonopsis pilosula, 250g of radix pseudostellariae, 150g of gynostemma pentaphyllum, 150g of liquorice and 150g of moutan bark, cleaning, packaging with a sterilized gauze bag, putting into a vacuum homogenizing emulsifying pot, adding 10 times of pure water according to the weight of the traditional Chinese medicine composition, extracting for 1 hour at 95-100 ℃, and filtering to obtain medicinal juice, thus obtaining the aqueous extract of the traditional Chinese medicine composition.

The formula of the aqueous extract repair emulsion containing 25 wt% of the traditional Chinese medicine composition is shown in table 3:

TABLE 3

The components of the formulation of table 3 were prepared according to the following process:

(2, mixing the components of the phase A, heating to 82 ℃, and uniformly stirring;

The efficacy of examples 1 to 3 is as follows:

in the embodiments 1-3, the data of 48 testers observed and recorded are as follows:

(1) the age of 48 testers is between 30 and 68 years old, wherein 12 male testers and 36 female testers are selected. The facial skin was typed according to the presence of wrinkles as follows, with reference to the glotauau typing method for clinical typing of skin photoaging: wherein 12 human skin wrinkles are type I skin on the move; wherein 18 human skin wrinkles are type II skin at rest; of which 18 human skin wrinkles are densely distributed as type III skin. The above individuals of each skin type were equally divided into A, B, C groups: group a used the repair serum prepared in example 1; group B repair cream prepared using example 2; group C the repair emulsion prepared in example 3 was used.

(2) The using method comprises the following steps: the product of the invention is taken to clean the face, and is used once in the morning and at night for ninety days.

(3) Effect judgment standard:

the effect is shown: wrinkles are remarkably disappeared or reduced;

the method has the following advantages: partial disappearance or reduction of wrinkles;

and (4) invalidation: no change, or no change in wrinkles was evident.

And (3) test results:

table 4: the repairing effect of the group a on facial wrinkles using the repairing essence prepared in example 1 is as follows:

table 5: group B the effect of the repair cream prepared using example 2 on the repair of facial wrinkles is as follows:

skin type	Number of people	Show effect	Is effective	Invalidation	Significant efficiency + effective rate
						Type I skin	4	1	1	2	50.0％
Type II skin	6	2	3	1	83.3％
						Type III skin	6	3	1	2	66.7％

Table 6: group C the effect of repairing facial wrinkles using the repair lotion prepared in example 3 is as follows:

skin type	Number of people	Show effect	Is effective	Invalidation	Significant efficiency + effective rate
						Type I skin	4	3	0	1	75.0％
Type II skin	6	4	2	0	100％
						Type III skin	6	3	2	1	83.3％

Conclusion 1: the water extract of the traditional Chinese medicine composition has obvious effect of repairing facial wrinkles when being applied to cosmetics.

Conclusion 2: for mild wrinkles, i.e. type I skin; medium wrinkles, i.e. type II skin; the use conditions of the severe wrinkles, namely the type III skin of each group of people can show that the three cosmetics prepared in the examples 1-3 can achieve the really effective repairing effect, and the obvious effect rate and the effective rate are 50.0 percent at least and 100 percent at most.

Example 4: is the application of the traditional Chinese medicine fermentation product in the repair essence.

Preparing a traditional Chinese medicine fermentation product: weighing 200g of codonopsis pilosula, 200g of radix pseudostellariae, 100g of gynostemma pentaphyllum, 100g of liquorice and 100g of moutan bark, cleaning, placing in a fermentation tank, adding 100g of high-activity dry yeast and 10 times of pure water, fermenting for 4 hours at 42 ℃ and 0rpm, sterilizing, filtering to obtain filtrate, and thus obtaining the traditional Chinese medicine fermentation product.

The formula of the repair essence containing 20 wt% of the fermented product of the traditional Chinese medicine is shown in table 7:

TABLE 7

The components of the formulation of table 7 were prepared according to the following procedure:

Example 5: is the application of Chinese medicinal fermented product in repairing cream.

Preparing a traditional Chinese medicine fermentation product: weighing 350g of codonopsis pilosula, 350g of radix pseudostellariae, 200g of gynostemma pentaphyllum, 200g of liquorice and 200g of moutan bark, cleaning, placing in a fermentation tank, adding 350g of high-activity dry yeast and 10 times of pure water, fermenting for 8 hours at 36 ℃ and 10rpm, sterilizing, filtering to obtain filtrate, and thus obtaining the traditional Chinese medicine fermentation product.

The formula of the repair cream containing 15 wt% of the fermented product of the traditional Chinese medicine is shown in table 8:

TABLE 8

The components of the formulation of table 8 were prepared according to the following procedure:

(2) mixing the phase A components, heating to 82 ℃, and uniformly stirring;

Example 6: is the application of Chinese medicine leavening in repairing emulsion.

Preparing a traditional Chinese medicine fermentation product: weighing 300g of codonopsis pilosula, 250g of radix pseudostellariae, 150g of gynostemma pentaphyllum, 150g of liquorice and 150g of moutan bark, cleaning, placing in a fermentation tank, adding 220g of high-activity dry yeast and 10 times of pure water, fermenting for 24 hours at 28 ℃ and 5rpm, sterilizing, filtering to obtain filtrate, and thus obtaining the traditional Chinese medicine fermentation product.

The formula of the repair emulsion containing 25 wt% of the fermented product of the traditional Chinese medicine is shown in table 9:

TABLE 9

Table 9 the ingredients in the formulation were prepared according to the following process:

The efficacy of examples 4 to 6 is as follows:

in the embodiments 4-6, the data of 48 testers observed and recorded are as follows:

(1) the age of 48 testers is between 30 and 68 years old, wherein 12 male testers and 36 female testers are selected. The facial skin was typed according to the presence of wrinkles as follows, with reference to the glotauau typing method for clinical typing of skin photoaging: wherein 12 human skin wrinkles are type I skin on the move; wherein 18 human skin wrinkles are type II skin at rest; of which 18 human skin wrinkles are densely distributed as type III skin. The above individuals of each skin type were equally divided into A, B, C groups: group a used the repair serum prepared in example 4; group B the repair cream prepared in example 5 was used; group C the repair emulsion prepared in example 6 was used.

(3) Effect judgment standard:

the effect is shown: wrinkles are remarkably disappeared or reduced;

and (4) invalidation: no change, or no change in wrinkles was evident.

And (3) test results:

table 10: the repairing effect of the group A on the facial wrinkles by using the repairing essence prepared in example 4 is as follows:

skin type	Number of people	Show effect	Is effective	Invalidation	Significant efficiency + effective rate
						Type I skin	4	2	2	0	100％
Type II skin	6	2	3	1	83.3％
						Type III skin	6	3	2	1	83.3％

Table 11: group B the effect of the repair cream prepared using example 5 on the repair of facial wrinkles is as follows:

skin type	Number of people	Show effect	Is effective	Invalidation	Significant efficiency + effective rate
						Type I skin	4	1	2	1	75.0％
Type II skin	6	2	2	2	66.7％
						Type III skin	6	1	2	3	50.0％

Table 12: group C the effect of repairing facial wrinkles using the repair lotion prepared in example 6 is as follows:

skin type	Number of people	Show effect	Is effective	Invalidation	Significant efficiency + effective rate
						Type I skin	4	1	3	0	100％
Type II skin	6	3	2	1	83.3％
						Type III skin	6	1	2	3	50.0％

Conclusion 1: the fermented product of the traditional Chinese medicine composition has obvious effect of repairing facial wrinkles when being applied to cosmetics.

Conclusion 2: aiming at the use conditions of people in each group of mild wrinkles, namely type I skin, moderate wrinkles, namely type II skin and severe wrinkles, namely type III skin, the three cosmetics prepared in the examples 4 to 6 can achieve a really effective repairing effect, and the obvious effect rate and the effective rate are 50.0 percent at the lowest and 100 percent at the highest.

The names of the cosmetic ingredients according to examples 1 to 6 are listed in the catalog of names of used cosmetic ingredients issued by the State food and drug administration (2015 edition).

In the above embodiments, the Chinese medicinal composition is a crude medicinal material or a Chinese medicinal decoction piece. A Chinese medicinal composition with skin aging delaying effect, and its ferment and application, comprises water extract and Chinese medicinal ferment of Chinese medicinal composition, and application of the water extract and Chinese medicinal ferment in preparing medicine or cosmetic with skin aging delaying, antiaging, wrinkle repairing, moisturizing, antioxidant, free radical scavenging, antiinflammatory, and skin aging delaying effects.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims

1. A traditional Chinese medicine composition with the effect of delaying skin aging comprises the following components in parts by mass: 200-350 parts of codonopsis pilosula, 200-350 parts of radix pseudostellariae, 100-200 parts of gynostemma pentaphylla, 100-200 parts of liquorice and 100-200 parts of moutan bark.

2. The traditional Chinese medicine composition with the effect of delaying skin aging according to claim 1, which is characterized by comprising the following components in parts by mass: 200 parts of codonopsis pilosula, 200 parts of radix pseudostellariae, 100 parts of gynostemma pentaphylla, 100 parts of liquorice and 100 parts of moutan bark.

3. The traditional Chinese medicine composition with the effect of delaying skin aging according to claim 1, which is characterized by comprising the following components in parts by mass: 350 parts of codonopsis pilosula, 350 parts of radix pseudostellariae, 200 parts of gynostemma pentaphylla, 200 parts of liquorice and 200 parts of moutan bark.

4. The traditional Chinese medicine composition with the effect of delaying skin aging according to claim 1, which is characterized by comprising the following components in parts by mass: 300 parts of codonopsis pilosula, 250 parts of radix pseudostellariae, 150 parts of gynostemma pentaphylla, 150 parts of liquorice and 150 parts of moutan bark.

5. The aqueous extract of the Chinese medicinal composition according to any one of claims 1 to 4, which is a filtrate obtained by adding 10 times of pure water by weight to the Chinese medicinal composition, extracting the mixture in a vacuum homogenizing and emulsifying pot at 95 to 100 ℃ for 1 hour, and filtering the mixture.

6. A fermented product of the Chinese medicinal composition according to any one of claims 1 to 4, which is a filtrate obtained by adding 10 times by weight of purified water to an initial system obtained by mixing the Chinese medicinal composition with fermentation tubes, fermenting the fermented product, and sterilizing and filtering the fermented product.

7. The fermented product of a Chinese medicinal composition according to claim 6, wherein the fermentation bacteria is yeast, and the Chinese medicinal composition is added into the fermented product in the form of high-activity dry yeast according to 100-350 parts by mass.

8. The fermented product of the Chinese medicinal composition according to claim 6, wherein the temperature in the fermentation step is 25-45 ℃; the stirring speed is 0-50 rpm; the time is 4-24 h.

9. Use of the aqueous extract of the Chinese medicinal composition for delaying skin aging according to claim 5, which is incorporated into cosmetics in an amount of 1 to 60% by weight in the preparation of cosmetics for delaying skin aging.

10. Use of a fermented product of the Chinese medicinal composition with skin aging delaying effect according to any one of claims 6 to 8 in the preparation of a cosmetic with skin aging delaying effect, which is characterized in that the fermented product is added into the cosmetic in a proportion of 1 to 60 percent by weight.