CN102057777A - Method for breaking Thesium chinense seed dormancy for promoting germination - Google Patents
Method for breaking Thesium chinense seed dormancy for promoting germination Download PDFInfo
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- CN102057777A CN102057777A CN 201010522602 CN201010522602A CN102057777A CN 102057777 A CN102057777 A CN 102057777A CN 201010522602 CN201010522602 CN 201010522602 CN 201010522602 A CN201010522602 A CN 201010522602A CN 102057777 A CN102057777 A CN 102057777A
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Abstract
The invention discloses a method for breaking Thesium chinense seed dormancy for promoting germination. The germination stage of Thesium chinense seeds is put ahead greatly by breaking the Thesium chinense seed dormancy by a method of soaking the seeds by using 500 mgL<-1> of GA3 for 24 hours and laminating on wet sand at a low temperature for 150 days. Therefore, the problems that the Thesium chinense seeds are in deep dormancy and are difficult to germinate are solved; and powerful technical support is provided for the development of wild tending, introducing and planting the Thesium chinense.
Description
Technical field
The present invention relates to a kind of method of abolishing Thesium chinese seed dormancy stratification, belong to traditional Chinese medicine culture technique field.
Background technology
Thesium chinese Thesium chinense Turcz. is the perennial partial parasite of Santalaceae, and its dry herb is China's Chinese herbal medicine commonly used among the people, has clearing heat and detoxicating, the puckery essence of kidney tonifying, the effect of relieving summer-heat dampness removing.Modern study shows that Thesium chinese has remarkable anti-inflammatory, analgesia and broad-spectrum antibacterial action, is described as " bouvardin ".Be used for the treatment of diseases such as pneumonia, mastitis, tonsillitis, sphagitis, tracheitis, rhinitis, cold, fever.The BAIRUI PIAN, BAIRUI JIAONANG etc. that with the Thesium chinese are raw material have been listed in national Chinese medicine protection kind.
In recent years, owing to reasons such as transition is excavated, environmental disruption and the semiparasitic biological natures of Thesium chinese, Thesium chinese wild resource reserves and apparent availability just are being the trend that falls sharply year by year, far can not satisfy the medication demand, Thesium chinese is wild to be fostered, or by introduction and acclimatization realization artificial cultivation, imperative.Thesium chinese mainly relies on seminal propagation, but behind its seed maturity, is arranged the very long deep-sleep phase, and next year March to April just begin rudiment to the maturation in May annual April under the natural conditions, and 10～11 months resting stages, and it is extremely irregular to emerge, germination rate, emergence rate are low.The relevant at present Thesium chinese seed dormancy of breaking promotes the method for seed sprouting not see open report as yet.
The invention provides a kind of method of abolishing Thesium chinese seed dormancy stratification,, promote that Sustainable utilization of resources is significant for improving the Thesium chinese reproduction coefficient, protecting its wild resource.
Summary of the invention
The object of the present invention is to provide a kind of method of abolishing Thesium chinese seed dormancy stratification, with the deep-sleep that solves the Thesium chinese seed, the difficult problem of germinateing, make its Seed Dormancy time 10～11 months under the natural conditions shorten to 150 days, germination rate reaches 68%.
A kind of method of abolishing Thesium chinese seed deep-sleep stratification provided by the present invention comprises:
A. seed collection: with Thesium chinese (Thesium chinense Turcz.) the seed natural air drying that collects, remove impurity and empty flat seed after, the seed of the mature and plump of selecting and remain is standby;
B. seed pre-treatment: flowing water flushing Thesium chinese seed 24h, use 500mgL again
-1GA
3Aqueous solution seed soaking bubble 24h dries the surface of the seed moisture after pulling out;
C. low temperature sand is hidden lamination: select evengranular river sand to clean, dry, in 110 ℃ of baking ovens, naturally cool to room temperature behind the baking 4h, to mix through the Thesium chinese seed after step B handles and river sand ratio in 1: 3, water content of substrate places 1～5 ℃ of refrigerator lamination to handle 150d with " hold agglomerating, as to loose one's grip and promptly loose " be advisable (being about 2%);
D. seed germination: the seed after the lamination processing finished places cultivation 20 ℃ of dark conditions under, and percentage of seedgermination can reach 68%.
The invention has the advantages that:
After the husky Tibetan of the low temperature lamination processing by the present invention's employing, effectively tear Thesium chinese Seed Dormancy, stratification, shorten resting stage, resting stage of 10～11 months under the natural conditions was foreshortened to about 150 days, germination rate is carried to 68%, realize Thesium chinese seed germination and the uniformity of emerging, improve the regularity of plant strain growth, for wild the fostering with introducing and planting of Thesium chinese provides strong technical guarantee.
Description of drawings
Fig. 1 is the influences of different lamination times to the Thesium chinese percentage of seedgermination
Embodiment
Test example 1
One, seed collection: with the Thesium chinese seed natural air drying that collects, behind removal impurity and the empty flat seed, the seed of the mature and plump of selecting and remain is standby;
Two, seed pre-treatment: get the seed of storage, at room temperature flowing water flushing 24h pulls out and dries the surface of the seed moisture, at ambient temperature, puts into concentration respectively and be 0,50,500 and 1000gmL
-1Soak 24h in the Gibberellins solution;
Three, the husky lamination of hiding of low temperature: select evengranular river sand to clean, dry, in 110 ℃ of baking ovens, naturally cool to room temperature behind the baking 4h, to mix through the Thesium chinese seed after step B handles and river sand ratio in 1: 3, water content of substrate places 1～5 ℃ of refrigerator lamination to handle 30d, 60d, 90d, 120d, 150d, 180d, 210d with " hold agglomerating, as to loose one's grip and promptly loose " be advisable (being about 2%);
Four, seed germination
A. seed splits shell rate statistics: 100 of the seed picked at random after the lamination processing is finished, distilled water flushing are to clean, and the counting seed splits hull number, according to splitting shell rate=split shell seed number/total seed number * 100% statistics;
B. percentage of seedgermination statistics: get and split the shell kind and place the 9cm culture dish that is covered with two layers of filter paper, add 4mL distilled water, under 20 ℃ of dark conditions, carry out sprouting test, germination rate=germinative number/total seed number * 100%.
Its effect is:
Table 1 Thesium chinese seed splits the variation of shell rate with the lamination time
Annotate: subscript or contain different alphabetical persons with subscript in the delegation and be significant difference (α=0.05) in the same row.
After the Thesium chinese earthing of seeds, persitent perianth comes off, and plants the thickness of the shell attenuation, and irregular block breakage or crack appear in the surface.Low temperature lamination different time splits the shell rate to the Thesium chinese seed appreciable impact, and with the prolongation with the lamination time, seed splits the shell rate and improves gradually.0gmL
-1The gibberellin treatment lamination handles to 120d that seed splits the shell rate and germination rate is respectively 11.30% and 40.36%, handles to 150d that seed splits the shell rate and germination rate is respectively 15.28% and 66.67%; 50gmL
-1The gibberellin treatment lamination handles to 120d that seed splits the shell rate and germination rate is respectively 11.50% and 51.35%, handles to 150d that seed splits the shell rate and germination rate is respectively 17.50% and 72.22%; 500gmL
-1The gibberellin treatment lamination handles to 120d that seed splits the shell rate and germination rate is respectively 14.50% and 53.55%, handles to 150d that seed splits the shell rate and germination rate is respectively 22.00% and 75.00%; 1000gmL
-1The gibberellin treatment lamination handles to 120d that seed splits the shell rate and germination rate is respectively 0.84% and 0.11%, handles to 150d that seed splits the shell rate and germination rate is respectively 2.00% and 2.00%.During lamination 120d, it is the strongest to split the shell seed vigor, surpasses 150d and partly splits the shell seed and begin to lose vitality, downright bad phenomenon occurs, shows as the endosperm deliquescing, color becomes yellow by milky white, and therefore, selection 150d is a Best Times.
Specific embodiment 1
A. seed collection: will collect (collecting location?) Thesium chinese seed natural air drying, remove impurity and empty flat seed after, the seed of the mature and plump of selecting and remain is standby;
B. seed pre-treatment: flowing water flushing Thesium chinese seed 24h, use 500mgL again
-1GA
3Aqueous solution seed soaking bubble 24h dries the surface of the seed moisture after pulling out;
C. low temperature sand is hidden lamination: select evengranular river sand to clean, dry, in 110 ℃ of baking ovens, naturally cool to room temperature behind the baking 4h, to mix through the Thesium chinese seed after step B handles and river sand ratio in 1: 3, water content of substrate is advisable with " holding agglomerating; loose one's grip and promptly loose ", places 1～5 ℃ of refrigerator lamination to handle 150d;
D. seed germination: the seed after the lamination processing finished places cultivation 20 ℃ of dark conditions under, and percentage of seedgermination can reach 68%.
Claims (2)
1. abolish the seed dormant method of Thesium chinese for one kind, comprising:
A. seed collection: with Thesium chinese (Thesium chinense Turcz.) the seed natural air drying that collects, remove impurity and empty flat seed after, the seed of the mature and plump of selecting and remain is standby;
B. seed pre-treatment: flowing water flushing Thesium chinese seed 24h, use 500mgL again
-1GA
3Aqueous solution seed soaking bubble 24h dries the surface of the seed moisture after pulling out;
C. low temperature sand is hidden lamination: select evengranular river sand to clean, dry, in 110 ℃ of baking ovens, naturally cool to room temperature behind the baking 4h, to mix through the Thesium chinese seed after step B handles and river sand ratio in 1: 3, water content of substrate places 1～5 ℃ of refrigerator lamination to handle 150d with " hold agglomerating, as to loose one's grip and promptly loose " be advisable (being about 2%);
D. seed germination: the seed after the lamination processing finished places cultivation 20 ℃ of dark conditions under, and percentage of seedgermination can reach 68%.
2. a kind of Thesium chinese seed that the seed dormant method of Thesium chinese obtains of abolishing according to claim 1 is characterized in that: cultivate germination rate by the thermophilic of the Thesium chinese seed of abolishing the acquisition of Thesium chinese seed dormancy method in the claim 1 and reach 68%.
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102498786A (en) * | 2011-10-28 | 2012-06-20 | 云南农业大学 | Method for improving germination of Yunnan coptis seeds |
| CN102792803A (en) * | 2011-05-27 | 2012-11-28 | 广州中一药业有限公司 | Method for promoting germination of seeds of Tulipa edulis (Miq.) Baker |
| CN103039249A (en) * | 2013-01-09 | 2013-04-17 | 甘肃省治沙研究所 | Cultivation method of seedlings of Corispermum patelliforme |
| CN103609226A (en) * | 2013-12-11 | 2014-03-05 | 福建农林大学 | Method for accelerating germination of ormosia microphylla seeds |
| CN103749135A (en) * | 2014-01-16 | 2014-04-30 | 深圳市华美欣药业有限公司 | Planting method of domestic or allopatric species of Thesium chinense |
| CN104012209A (en) * | 2014-06-26 | 2014-09-03 | 甘肃中医学院 | Method for promoting seeds of meconopsis integrifolia to germinate |
| CN104429561A (en) * | 2014-12-22 | 2015-03-25 | 南京农业大学 | Method for breaking dormancy of tulipa edulis bulbs and increasing yield |
| CN110100526A (en) * | 2019-05-24 | 2019-08-09 | 南京农业大学 | A kind of cultural method improving Thesium chinese survival rate |
| CN113396664A (en) * | 2021-06-23 | 2021-09-17 | 安徽九华华源药业有限公司 | Method for breaking dormancy of thesium Chinese seeds |
| CN113491223A (en) * | 2021-08-13 | 2021-10-12 | 安徽九华华源药业有限公司 | Method for breeding thesium Chinese seeds |
| CN115226434A (en) * | 2022-08-09 | 2022-10-25 | 陕西师范大学 | Method for shortening germination period of thesium chinense seeds and improving germination rate |
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| CN103548442B (en) * | 2013-11-07 | 2014-10-22 | 广西壮族自治区林业科学研究院 | Method for soaking castanopsis hystrix seeds in running water |
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| CN1686258A (en) * | 2005-04-07 | 2005-10-26 | 上海交通大学 | Thesium Chinese total flavone preparation method |
| CN1698758A (en) * | 2005-05-09 | 2005-11-23 | 贵阳云岩西创药物科技开发有限公司 | Pharmaceutical preparation of bastardtoad flax and its preparation method and quality control method |
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| CN1698758A (en) * | 2005-05-09 | 2005-11-23 | 贵阳云岩西创药物科技开发有限公司 | Pharmaceutical preparation of bastardtoad flax and its preparation method and quality control method |
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Cited By (19)
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| CN102792803A (en) * | 2011-05-27 | 2012-11-28 | 广州中一药业有限公司 | Method for promoting germination of seeds of Tulipa edulis (Miq.) Baker |
| CN102792803B (en) * | 2011-05-27 | 2013-11-13 | 广州白云山中一药业有限公司 | Method for promoting germination of seeds of Tulipa edulis (Miq.) Baker |
| CN102498786A (en) * | 2011-10-28 | 2012-06-20 | 云南农业大学 | Method for improving germination of Yunnan coptis seeds |
| CN103039249A (en) * | 2013-01-09 | 2013-04-17 | 甘肃省治沙研究所 | Cultivation method of seedlings of Corispermum patelliforme |
| CN103039249B (en) * | 2013-01-09 | 2014-05-07 | 甘肃省治沙研究所 | Cultivation method of seedlings of Corispermum patelliforme |
| CN103609226A (en) * | 2013-12-11 | 2014-03-05 | 福建农林大学 | Method for accelerating germination of ormosia microphylla seeds |
| CN103749135B (en) * | 2014-01-16 | 2015-09-23 | 香港九华华源集团滁州药业有限公司 | The implantation methods that Thesium chinese man plants or strange land is introduced a fine variety |
| CN103749135A (en) * | 2014-01-16 | 2014-04-30 | 深圳市华美欣药业有限公司 | Planting method of domestic or allopatric species of Thesium chinense |
| CN104012209A (en) * | 2014-06-26 | 2014-09-03 | 甘肃中医学院 | Method for promoting seeds of meconopsis integrifolia to germinate |
| CN104429561A (en) * | 2014-12-22 | 2015-03-25 | 南京农业大学 | Method for breaking dormancy of tulipa edulis bulbs and increasing yield |
| CN110100526A (en) * | 2019-05-24 | 2019-08-09 | 南京农业大学 | A kind of cultural method improving Thesium chinese survival rate |
| CN110100526B (en) * | 2019-05-24 | 2021-09-14 | 南京农业大学 | Cultivation method for increasing survival rate of thesium chinense |
| CN113396664A (en) * | 2021-06-23 | 2021-09-17 | 安徽九华华源药业有限公司 | Method for breaking dormancy of thesium Chinese seeds |
| CN113396664B (en) * | 2021-06-23 | 2022-02-18 | 安徽九华华源药业有限公司 | Method for breaking dormancy of thesium Chinese seeds |
| WO2022267655A1 (en) * | 2021-06-23 | 2022-12-29 | 安徽九华华源药业有限公司 | Method for breaking dormancy of seeds of thesium chinense turcz. |
| CN113491223A (en) * | 2021-08-13 | 2021-10-12 | 安徽九华华源药业有限公司 | Method for breeding thesium Chinese seeds |
| WO2023016000A1 (en) * | 2021-08-13 | 2023-02-16 | 安徽九华华源药业有限公司 | Thesium chinense turcz. seed propagation method |
| CN115226434A (en) * | 2022-08-09 | 2022-10-25 | 陕西师范大学 | Method for shortening germination period of thesium chinense seeds and improving germination rate |
| CN115226434B (en) * | 2022-08-09 | 2024-01-16 | 陕西师范大学 | Method for shortening germination period of bastardtoad seed and improving germination rate |
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