CN102049034B - Hoof nail polypeptide preparation for promoting tissue repair and preparation method thereof - Google Patents
Hoof nail polypeptide preparation for promoting tissue repair and preparation method thereof Download PDFInfo
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- CN102049034B CN102049034B CN 200910193572 CN200910193572A CN102049034B CN 102049034 B CN102049034 B CN 102049034B CN 200910193572 CN200910193572 CN 200910193572 CN 200910193572 A CN200910193572 A CN 200910193572A CN 102049034 B CN102049034 B CN 102049034B
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Abstract
The invention discloses a hoof nail polypeptide preparation for promoting tissue repair and a preparation method thereof. In the method, animal hoof extracts, chrysanthemums, common bletilla pseudo-bulbs, and hairyvein agrimonia herbs are taken as raw materials; and the method comprises the following steps: preparing the raw materials into active ingredients; and then preparing an oral and external preparation from the active ingredients and commonly-used pharmaceutical necessities. The preparation can promote tissue repair.
Description
Technical field
The present invention relates to a kind of hoof nail polypeptide preparation that promotes tissue repair, said preparation is made up of hoof nail polypeptide and Chinese crude drug extract, belongs to the field of Chinese medicines.
Technical background
Agglutination behind the tissue injury is counted as the process of various repairing cell proliferations, differentiation, migration, apoptosis and disappearance, is that series of different cell, structural protein, somatomedin and protein kinase etc. form the network type reciprocal action.Tissue repair is the repair process after the various damages of body, that is is that the integral body or the organ of organism receives external force effect generation wound and partial loss, in the process that grows again on the basis of remainder with lost part same structure on form and function.In the process of tissue reparation with propagation, differentiation and the inflammatory mediator of multiple repair cell, the release of cytokine, collagen hypertrophy.Impaired tissue types is different, and the repair cell kind is also inequality.
Peptide promptly is proteinic structure fragment, is amino acid whose organic synthesis thing, and the peptide that a plurality of aminoacid connect into through peptide bond is a polypeptide; Polypeptide is a kind of material that is between aminoacid and the protein, and it can deeply be failed nutrient to deep skin and organize, nutrition, moisten, repair, moist, compose living cells, environment in regulating strengthens body immunity, purifies the blood blood circulation promoting.
Unguis Sus domestica is used as medicine, and is promptly on the books in Shennong's Herbal; Compendium of Material Medica records " dewclaw first "; Other all on the books like " herbal classic ", " Mingyi Bielu " etc.The Bauhinia variegata Linn extract is extracting bioactive substance in pig, sheep or the Unguis Bovis seu Bubali, adopts prepared such as modern biotechnology to become the polypeptide of biologically active.
Summary of the invention
The object of the invention provides a kind of novel formulation that combines the Chinese crude drug extract to form as raw material with hoof nail polypeptide, and in conjunction with Chinese medical theory, through the rational proportion of each medical material, synergism promotes tissue repair, has good therapeutic effect.
Another one purpose of the present invention provides method of making preparation.
Preparation of the present invention selects hoof nail polypeptide, Flos Chrysanthemi, Pseudobulbus Bletillae (Rhizoma Bletillae) and Herba Agrimoniae to carry out the combined preparation active component; Wherein hoof nail polypeptide has enhance immunity, promotes the tissue repair effect; Flos Chrysanthemi sweet in the mouth, hardship, be slightly cold, return lung, Liver Channel, the wind heat clearing away of loosing, suppressing the hyperactive liver makes eye bright; Pseudobulbus Bletillae (Rhizoma Bletillae), bitter, sweet, puckery, cold, attach to the lung and stomach meridians, the tonifying the lung hemostasis, detumescence and promoting granulation is held back skin ulcer; Herba Agrimoniae, bitter in the mouth, puckery, flat is returned lung, liver, spleen channel, astringing to arrest bleeding, skin ulcer is treated in detoxifcation.Above-mentioned four Chinese medicine is shared to play the toxin expelling effect of invigorating blood circulation.Share in conjunction with hoof nail polypeptide, reach the differentiation of promotion repair cell hypertrophy, play repair to tissue injury.
The raw material consumption of preparation medicine activity component of the present invention is: hoof nail polypeptide 10-30 part, Flos Chrysanthemi 10-30 part, Pseudobulbus Bletillae (Rhizoma Bletillae) 10-30 part and Herba Agrimoniae 10-30 part; Be preferably 12 parts of hoof nail polypeptides, 12 parts of Flos Chrysanthemis, 12 parts of Pseudobulbus Bletillae (Rhizoma Bletillae), 12 parts of Herba Agrimoniaes.
Medicine of the present invention can adopt conventional method to be prepared into conventional oral formulations and external preparation, the tissue repair that receives external force effect generation wound to the integral body or the organ of organism.
Flos Chrysanthemi, Pseudobulbus Bletillae (Rhizoma Bletillae) and Herba Agrimoniae are added 9-10 times of water gaging soaked 2-3 hour, heating decocts, and the after-filtration that boils, medicinal residues add 6-8 times of water gaging again and be heated to and boil, the after-filtration that boils, filtrating merges, and separates solid content, supernatant; When supernatant concentration to relative density is 1.05-1.06, advance spray dryer, 140-160 ℃ of EAT control; Leaving air temp 100-110 ℃, 50-70 rev/min of nebulizer rotating speed, charging rate is 3-6ml/ minute; Get spray drying powder, pulverize, can get fine powder after sieving; Fine powder and hoof nail polypeptide powder mix homogeneously; The various conventional adjuvant of needs in the time of can adding the preparation different dosage form, the formulation method of routines such as lubricant, binding agent is prepared into common preparation formulation, like tablet, capsule, oral liquid, electuary etc.
Below in conjunction with embodiment the present invention is done further elaboration:
Embodiment 1 capsule preparation of the present invention (is example with ten times of material proportional quantities)
1. take by weighing 120 parts of each crude drug hoof nail polypeptides, 120 parts of Flos Chrysanthemis, 120 parts of Pseudobulbus Bletillae (Rhizoma Bletillae), 120 parts of Herba Agrimoniaes by following weight proportion, subsequent use;
2. above-mentioned weight Flos Chrysanthemi, Pseudobulbus Bletillae (Rhizoma Bletillae), Herba Agrimoniae add 9-10 times of water gaging and soaked 2-3 hour, and heating decocts, and the after-filtration that boils, medicinal residues add 6-8 times of water gaging again and be heated to and boil, the after-filtration that boils, filtrating merges, and separates solid content, supernatant;
3. supernatant concentration to relative density is 1.06 o'clock, advances spray dryer, 150 ℃ of EAT controls, and 105 ℃ of leaving air temps, 60 rev/mins of nebulizer rotating speeds, charging rate is 5ml/ minute, gets spray drying powder;
4. pulverize spray drying powder and become fine powder, behind the adding hoof nail polypeptide fine powder mixing, the snap fit capsule of packing into.
Embodiment 2: external liniment preparation of the present invention (is example with ten times of material proportional quantities)
1. take by weighing 120 parts of each crude drug hoof nail polypeptides, 120 parts of Flos Chrysanthemis, 120 parts of Pseudobulbus Bletillae (Rhizoma Bletillae), 120 parts of Herba Agrimoniaes by following weight proportion, subsequent use;
2. above-mentioned weight Flos Chrysanthemi, Pseudobulbus Bletillae (Rhizoma Bletillae), Herba Agrimoniae add 9-10 times of water gaging and soaked 2-3 hour, and heating decocts, and the after-filtration that boils, medicinal residues add 6-8 times of water gaging again and be heated to and boil, the after-filtration that boils, filtrating merges, and separates solid content, supernatant;
3. supernatant concentration to relative density is 1.06 o'clock, advances spray dryer, 150 ℃ of EAT controls, and 105 ℃ of leaving air temps, 60 rev/mins of nebulizer rotating speeds, charging rate is 5ml/ minute, gets spray drying powder;
4. pulverize spray drying powder and become fine powder, add hoof nail polypeptide fine powder mixing after, adds in 350 parts of glycerol and is warmed up to 45 ℃, stir abundant, be cooled to room temperature after, airtight preservation.
Experimental example 3 the present invention scald animal and repair the Ergonomy evaluation
Experiment material and method
Laboratory animal: 30 of male SD rats; Scale of construction 180-220 gram is all raised under cleaning ambient.
Experiment reagent: betagen solution, preparation of the present invention.
Animal divides into groups: 30 burned rats are a few days ago claimed the scale of construction, are divided into simple burn group, povidone iodine treatment group and preparation for treating group of the present invention, 10 every group at random by the scale of construction.
Animal model is set up: animal is scalded back depilation in preceding 1 day, adopts the sodium pentobarbital intraperitoneal injection of anesthesia during experiment, and the district of will losing hair or feathers places 70 degree waters bath with thermostatic control 15 seconds, confirms to process the shallow II degree of 10% body surface area scalding model through pathological section.The face of respectively forming covers with normal saline gauze, povidone iodine gauze, preparation gauze of the present invention respectively, puts back to sub-cage rearing in the cage after the sterile gauze wrapping is fixing, changes dressings 1 time/day.
Wound healing time: observe the wound healing situation every day, cover foundation as wound healing time with complete epithelium.
The wound healing rate: rat was observed the wound healing situation in 7,10,14 days in hindering the back, promptly at first the wound surface area was depicted on the transparent membrane, again as template, the uniform hard paper of quality was cut into onesize, claimed quality with analytical balance then.Represent wound surface area size indirectly with the hard paper quality.
By formula calculate the wound healing rate: wound healing rate (%)=(original wound surface area-the wound surface area does not heal)/original wound surface area
Experimental result
1. respectively organize the healing time comparative result, see table 1, the visible povidone iodine group of result relatively has significant difference P＜0.01 with of the present invention group and simple scald group from table.
Table 1 is respectively organized the scalding healing time ratio
The Δ representative relatively has significant difference P＜0.01 with the simple scald group
2. wound healing rate result sees table 2, and the result is visible from table relatively has significant difference P＜0.01 7,10,14 days three time period povidone iodine groups and of the present invention group with the simple scald group.
Table 2 is respectively formed the face healing rate relatively
The Δ representative relatively has significant difference P＜0.01 with the simple scald group.
Claims (6)
1. preparation that promotes tissue repair, the active component that it is characterized in that it is to be processed by the raw material of following weight portion: hoof nail polypeptide 10-30 part, Flos Chrysanthemi 10-30 part, Pseudobulbus Bletillae (Rhizoma Bletillae) 10-30 part and Herba Agrimoniae 10-30 part.
2. according to the preparation of claim 1, wherein the consumption of each raw material is: 12 parts of 12 parts of hoof nail polypeptides, 12 parts of Flos Chrysanthemis, 12 parts of Pseudobulbus Bletillae (Rhizoma Bletillae) and Herba Agrimoniaes.
3. the method for preparing of claim 1 or 2 described preparations comprises the steps:
1) it is subsequent use to take by weighing each crude drug hoof nail polypeptide, Flos Chrysanthemi, Pseudobulbus Bletillae (Rhizoma Bletillae) and Herba Agrimoniae.
2) add 9-10 times of water gaging according to Flos Chrysanthemi, Pseudobulbus Bletillae (Rhizoma Bletillae) and the Herba Agrimoniae of above-mentioned weight proportion and soaked 2-3 hour, heating decocts, and the after-filtration that boils, medicinal residues add 6-8 times of water gaging again and be heated to and boil, and filter, and filtrating merges, and separates solid content, supernatant;
When 3) supernatant concentration to relative density is 1.05-1.06, advance spray dryer, 140-160 ℃ of EAT control, leaving air temp 100-110 ℃, 50-70 rev/min of nebulizer rotating speed, charging rate is 3-6ml/ minute, gets spray drying powder;
4) spray drying powder is pulverized, and sieves, and gets fine powder; Fine powder and hoof nail polypeptide mix homogeneously.
4. method for preparing according to claim 3 is used component and tablet that step 4) gets always the adjuvant mixed pressuring plate.
5. method for preparing according to claim 3 is mixed the component that step 4) gets with capsule adjuvant commonly used, encapsulated.
6. method for preparing according to claim 3 is mixed the component that step 4) gets with external liniment adjuvant commonly used, can do the external liniment.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910193572 CN102049034B (en) | 2009-11-02 | 2009-11-02 | Hoof nail polypeptide preparation for promoting tissue repair and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910193572 CN102049034B (en) | 2009-11-02 | 2009-11-02 | Hoof nail polypeptide preparation for promoting tissue repair and preparation method thereof |
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| Publication Number | Publication Date |
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| CN102049034A CN102049034A (en) | 2011-05-11 |
| CN102049034B true CN102049034B (en) | 2012-12-26 |
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| CN 200910193572 Expired - Fee Related CN102049034B (en) | 2009-11-02 | 2009-11-02 | Hoof nail polypeptide preparation for promoting tissue repair and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103919834A (en) * | 2014-04-01 | 2014-07-16 | 唐登贵 | Traditional Chinese medicinal composition for treating soft tissue injury and preparation method thereof |
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2009
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Non-Patent Citations (2)
| Title |
|---|
| 张天民 等.蹄甲多肽的研究概况与展望.《中国生化药物杂志》.2005,第26卷(第6期),369-371. * |
| 朱希强 等.猪蹄甲的药用概况.《氨基酸和生物资源》.2000,第22卷(第2期),43-46. * |
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