CN102042924B - 一种细胞或细菌的染色、制片方法 - Google Patents
一种细胞或细菌的染色、制片方法 Download PDFInfo
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- CN102042924B CN102042924B CN201010529398.XA CN201010529398A CN102042924B CN 102042924 B CN102042924 B CN 102042924B CN 201010529398 A CN201010529398 A CN 201010529398A CN 102042924 B CN102042924 B CN 102042924B
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- 238000004043 dyeing Methods 0.000 title claims abstract description 51
- 230000000844 anti-bacterial Effects 0.000 title claims abstract description 37
- 239000012528 membrane Substances 0.000 claims abstract description 64
- 239000007788 liquid Substances 0.000 claims abstract description 36
- 238000001914 filtration Methods 0.000 claims abstract description 34
- 239000012128 staining reagent Substances 0.000 claims abstract description 13
- 239000000758 substrate Substances 0.000 claims abstract description 8
- 238000000034 method Methods 0.000 claims description 12
- 238000002360 preparation method Methods 0.000 abstract description 5
- 238000010339 medical test Methods 0.000 abstract description 3
- 230000000717 retained Effects 0.000 abstract 1
- 238000011049 filling Methods 0.000 description 9
- 239000002699 waste material Substances 0.000 description 7
- 239000003153 chemical reaction reagent Substances 0.000 description 6
- 230000000694 effects Effects 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 238000004140 cleaning Methods 0.000 description 3
- 230000029087 digestion Effects 0.000 description 3
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- 238000010186 staining Methods 0.000 description 3
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 101700074463 DERL1 Proteins 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 239000011229 interlayer Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 210000004369 Blood Anatomy 0.000 description 1
- 210000003097 Mucus Anatomy 0.000 description 1
- 206010036790 Productive cough Diseases 0.000 description 1
- 210000003802 Sputum Anatomy 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 210000002249 digestive system Anatomy 0.000 description 1
- 230000003419 expectorant Effects 0.000 description 1
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- 238000005755 formation reaction Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000010410 layer Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
Abstract
Description
Claims (3)
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201010529398.XA CN102042924B (zh) | 2010-10-30 | 一种细胞或细菌的染色、制片方法 | |
PCT/CN2010/001916 WO2012055077A1 (zh) | 2010-10-30 | 2010-11-29 | 细胞或细菌的染色制片方法及装置 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201010529398.XA CN102042924B (zh) | 2010-10-30 | 一种细胞或细菌的染色、制片方法 |
Publications (2)
Publication Number | Publication Date |
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CN102042924A CN102042924A (zh) | 2011-05-04 |
CN102042924B true CN102042924B (zh) | 2016-12-14 |
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Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5269999A (en) * | 1992-03-31 | 1993-12-14 | Gas Research Institute | Preparation of core samples |
US5830413A (en) * | 1995-02-25 | 1998-11-03 | Boehringer Mannheim Gmbh | Device for processing slide-mounted samples |
JP2003114175A (ja) * | 2001-07-30 | 2003-04-18 | Matsushita Ecology Systems Co Ltd | 微生物採取チップ、微生物採取キット、微生物計量方法、微生物計量装置の正常状態確認検査用検体及び微生物計量装置 |
CN2786610Y (zh) * | 2005-04-22 | 2006-06-07 | 彭钧 | 隔层式细胞、细菌涂片装置 |
CN2887428Y (zh) * | 2006-03-27 | 2007-04-11 | 李京考 | 一种细胞过滤器 |
CN201075067Y (zh) * | 2007-09-27 | 2008-06-18 | 彭钧 | 一种隔层式细胞、细菌涂片杯 |
CN101755198A (zh) * | 2007-05-31 | 2010-06-23 | 3M创新有限公司 | 用于收集和浓缩用于微生物分析的样品的装置及方法 |
Patent Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5269999A (en) * | 1992-03-31 | 1993-12-14 | Gas Research Institute | Preparation of core samples |
US5830413A (en) * | 1995-02-25 | 1998-11-03 | Boehringer Mannheim Gmbh | Device for processing slide-mounted samples |
JP2003114175A (ja) * | 2001-07-30 | 2003-04-18 | Matsushita Ecology Systems Co Ltd | 微生物採取チップ、微生物採取キット、微生物計量方法、微生物計量装置の正常状態確認検査用検体及び微生物計量装置 |
CN1537224A (zh) * | 2001-07-30 | 2004-10-13 | ���»���ϵͳ��ʽ���� | 微生物收集芯片、微生物收集试剂盒、微生物计量方法、微生物计量装置的正常状态检查确认用检体以及微生物计量装置 |
CN2786610Y (zh) * | 2005-04-22 | 2006-06-07 | 彭钧 | 隔层式细胞、细菌涂片装置 |
CN2887428Y (zh) * | 2006-03-27 | 2007-04-11 | 李京考 | 一种细胞过滤器 |
CN101755198A (zh) * | 2007-05-31 | 2010-06-23 | 3M创新有限公司 | 用于收集和浓缩用于微生物分析的样品的装置及方法 |
CN201075067Y (zh) * | 2007-09-27 | 2008-06-18 | 彭钧 | 一种隔层式细胞、细菌涂片杯 |
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Address after: 410205 Hunan province Changsha Valley High Tech Development Zone No. 229 yuan Lu, Hunan Lugu international medical equipment Industrial Park A5 building 601 Applicant after: Hunan Tianqi Medical New Technology Co., Ltd. Address before: 410013 Hunan province Changsha 2 Fenglin Road No. 366 building 3 room 1609 unit margin Applicant before: Hunan Tianqi Medical New Technology Co., Ltd. |
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CB02 | Change of applicant information |
Address after: 410205 Hunan province Changsha Valley High Tech Development Zone No. 229 yuan Lu, Hunan Lugu international medical equipment Industrial Park A5 building 601 Applicant after: Hunan Tianqi Medical New Technology Co., Ltd. Address before: 410205 Hunan province Changsha Valley High Tech Development Zone No. 229 yuan Lu, Hunan Lugu international medical equipment Industrial Park A5 building 601 Applicant before: Hunan Tianqi Medical New Technology Co., Ltd. |
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