CN102040406B - Method for producing compound microbial fertilizer from amino acid organic granules - Google Patents
Method for producing compound microbial fertilizer from amino acid organic granules Download PDFInfo
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- CN102040406B CN102040406B CN 201010504067 CN201010504067A CN102040406B CN 102040406 B CN102040406 B CN 102040406B CN 201010504067 CN201010504067 CN 201010504067 CN 201010504067 A CN201010504067 A CN 201010504067A CN 102040406 B CN102040406 B CN 102040406B
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Abstract
The invention provides a method for producing compound microbial fertilizer from amino acid organic granules, in particular to a method for producing compound microbial fertilizer by adopting slurry spraying and granulating processes, comprising the following steps: preparing liquid microbial agent, granulating waste amino acid solution into organic granules, and compounding the organic granules with the liquid compound microbial agent, wherein the liquid compound microbial agent is uniformly absorbed on the surfaces of the amino acid organic granules, and fermentation liquid accounts for 0.5-1.5wt% of the finished product of the compound microbial fertilizer. According to the invention, the normal method for producing the compound microbial fertilizer is improved, a novel method for producing the granular compound microbial fertilizer by two steps of granulating waste amino acid solution into organic granules and absorbing the liquid compound microbial agent by the amino acid organic granules is provided, and the number of viable bacteria in the compound microbial fertilizer is ensured. The production of the compound microbial fertilizer by the slurry spraying and granulating processes breaks through the production of the powdery compound microbial fertilizer by the disc and roller granulating process, thus a good foundation is laid for improving the commodity value, effectiveness and large-area popularization of the compound microbial fertilizer.
Description
Technical field
The present invention relates to a kind of production method of novel particle composite microbiological fertilizer, specifically, relate to a kind of production method and application that utilizes the span amino acid mother liquor that produces in L-glutamic acid or monosodium glutamate (L-glutamic acid is the main raw material(s) of the producing monosodium glutamate) production process to produce high-efficiency environment friendly particle composite microbe fertilizer by the amino acid organic granular agent behind the spray granulating.
Background technology
Since using of a large amount of chemical fertilizer, soil compaction, deteriorating water quality, the agricultural-food severe contamination, whole farmland ecological environment and people's living environment is affected, and Sustainable development how to keep China's agricultural has caused the great attention of governments at all levels.Particularly after the China joined WTO; China's agricultural is subject to severe challenge; develop the outlet agriculture oriented for export and foreign exchange earnings; must carry forward vigorously the development of green food and pollution-free food; and under the impact of international financial crisis, traditional Fertilizer Industry is got deeply stuck in quagmire, and new-type fertilizer has but had good development space; tracing it to its cause is because " newly " in the new-type fertilizer, new science and technology, new fertilising theory and new product advantage.As the new-type fertilizer microbial fertilizer of production pollution-free food production data, become Ministry of Agriculture's emphasis and promoted product.But because microbial fertilizer is with high content of technology, production difficulty is large, and work of high quality also seldom on China market.The tool authoritative department is estimated: in 5 years of future, the annual requirement of microbial fertilizer will reach 30% of fertilizer amount, market capacity will reach the forty-two million ton, and 3,000,000 tons of present year output less thaies of China's microbial fertilizer, needs that far away can not satisfying the market.
Composite microbiological fertilizer is according to rhizosphere soil microecology and the plant principle of science of earning a living, take the active bacterium of multifunctional microbial (vinelandii, phosphate solubilizing bacteria, potassium bacterium) as core, take fertilizer type organic matter as carrier, having of being equipped with again that a small amount of inorganic nutrients and trace element process is pollution-free, nuisanceless, is suitable for producing the new-type fertilizer of green food.This new-type fertilizer can be to the farm crop fertilizer, can draw to crop rhizosphere soils again to be implanted with the beneficial microorganism dominant group, reaches the purpose of the strong sprout of fertilizing the soil, short biological and ecological methods to prevent plant disease, pests, and erosion disease and good quality and high output.According to the requirement of the composite microbiological fertilizer industry standard NY/T798-2004 of the Ministry of Agriculture, living bacteria count is the key technical indexes of this product, and the living bacteria count amount must be greater than 2,000 ten thousand/gram, total nutrient (N+P
2O
5+ K
2O) must be greater than 6%.But guarantee high number of viable, the support material that accounts for formula for a product ratio 70%-80% is the principal element that affects number of viable.Produce in the market the composite microbiological fertilizer product and mainly contain following technical problem:
1, mainly utilize the peat composed of rotten mosses or humic acids as organic carrier.Contain a large amount of organic matters in the peat composed of rotten mosses and the humic acids, C/N can guarantee the number of viable of microorganism than the life condition that meets microorganism.But the peat composed of rotten mosses and humic acids belong to mineral substance, be subjected to the restriction of national resources, a large amount of exploitations can destroy ecological, and execute in soil and to be difficult to dissolving, organic matter wherein and humic acids are difficult to by crop utilization, affect fertilizer efficiency, the price of the peat composed of rotten mosses and humic acids also goes up year by year simultaneously, and the production cost of product is also improving year by year.
2, product forms is mainly take powdery as main.Because contain viable bacteria in the composite microbiological fertilizer, the oven dry in the granulation process has a great impact number of viable, and because the peat composed of rotten mosses and humic acids belong to mineral attitude organic raw material, indissoluble solution more after the granulation, grain affects quality and the effect of product.But a lot of local arable land the in the north belongs to machine sowing, and powder-like product can't use, so granulating not directly has influence on using in a large number and promoting of product.
3, low with disk and drum granulating balling ratio, particle is irregular, and is rough, affected the visual appearance of product.
Summary of the invention
Therefore, the technical problem to be solved in the present invention provides production method a kind of cheapness, particle composite microbiological fertilizer that biological activity is high, solubility, and the method is high, the Environmental Safety of resource utilization simultaneously.
Technical scheme of the present invention is, the method for composite microbiological fertilizer is produced in a seed amino acid organic granular agent, comprises the preparation of microbial inoculum, and the method may further comprise the steps:
(1) liquid bacterial agent is the preparation of fermented liquid: will utilize for the microbial bacteria of fertilizer field the fermentation equipment fermentative production, and obtain fermented liquid, total living bacteria count reaches 25-60 hundred million/gram in the described fermented liquid;
(2) granulation: the amino acid waste liquid is through the agent of spray granulating granulation amino acid organic granular;
(3) amino acid organic granular agent inoculation: in the stirrer that amino acid organic granular agent adding material continuous uniform is flowed, add the fermented liquid that step (1) obtains with spray form, then air-dry or dry, obtain the composite microbiological fertilizer of finished product;
Described fermented liquid add-on accounts for the 0.5%-1.5% of finished product composite microbiological fertilizer gross weight.
The basic proterties of amino acid organic granular agent sees Table 1, and Fig. 1 is seen in concrete Production Flow Chart.
Table 1: amino acid organic granular agent proterties
As can be seen from Table 1, the total nutrient content (N+P of amino acid organic granular agent
2O
5+ K
2O) % has reached the requirement of total nutrient in " composite microbiological fertilizer " agricultural industry criteria greater than 6%.
Preferably, described microbial bacteria for fertilizer field is selected from subtilis (Bacillus subtilis), one or more in bacillus megaterium (Bacillus megaterium) and the Bacillus mucilaginosus (Bacillus mucilagimosus krassilm).The microbial bacteria that is used for fertilizer field involved in the present invention is not limited to above three kinds, can select different microbe species according to different soil and the demand of crop, as adopting vinelandii etc.The kind of selected microbial bacteria and ratio should make total living bacteria count of the every gram fermented liquid in its fermented liquid after fermentation reach hundred million of 25-60, then when liquid fermentation liquid is inoculated into amino acid organic granular agent step, determine the weight ratio that fermented liquid adds according to the viable count in the fermented liquid, make the finished product meet the requirement of " composite microbiological fertilizer " agriculture standard (NY/T798-2004).
For example, when using separately subtilis (Bacillus subtilis), the fermentation of bacillus subtilis liquid of adding accounts for the 0.5%-0.8% of finished product composite microbiological fertilizer gross weight; During separately with bacillus megaterium (Bacillus megaterium), the bacillus megaterium fermented liquid of adding accounts for the 0.6%-1.0% of finished product composite microbiological fertilizer gross weight; During separately with Bacillus mucilaginosus (Bacillus mucilagimosus krassilm), the Bacillus mucilaginosus fermented liquid of adding accounts for the 0.8%-1.5% of finished product composite microbiological fertilizer gross weight; With subtilis (Bacillus subtilis) and bacillus megaterium (Bacillus megaterium) two bacterial classifications difference fermentative production, two kinds of fermented liquids were mixed by weight 1: 1, and the mixed fermentation liquid of adding accounts for the 0.5%-0.9% of finished product composite microbiological fertilizer gross weight; With subtilis (Bacillus subtilis) and Bacillus mucilaginosus (Bacillus mucilagimosus krassilm) two bacterial classifications difference fermentative production, two kinds of fermented liquids were mixed by weight 2: 1, and the mixed fermentation liquid of adding accounts for the 0.6%-1.0% of finished product composite microbiological fertilizer gross weight; With bacillus megaterium (Bacillus megaterium) and Bacillus mucilaginosus (Bacillus mucilagimosus krassilm) two bacterial classifications difference fermentative production, two kinds of fermented liquids were mixed by weight 2: 1, and the mixed fermentation liquid of adding accounts for the 0.8%-1.2% of finished product composite microbiological fertilizer gross weight.Subtilis (Bacillus subtilis), bacillus megaterium (Bacillus megaterium) and Bacillus mucilaginosus (Bacillus mucilagimosus krassilm) be fermentative production respectively, three kinds of fermented liquids are mixed by weight 1-3: 0.5-2: 0.5-2, and the mixed fermentation liquid of adding accounts for the 0.5%-1.5% of finished product composite microbiological fertilizer gross weight.
Preferably, total living bacteria count reaches 30-50 hundred million/gram in the middle fermented liquid of described step (1), namely contains hundred million of effective viable count 30-50 in every gram fermented liquid.
Described amino acid waste liquid is the by product in L-glutamic acid and the glutamate production process.
Method according to composite microbiological fertilizer is produced in amino acid organic granular agent of the present invention is preferably, and the described stirrer of step (3) can be disk or rotary drum cylinder.Also can be other whipping devices that material continuous uniform is flowed.
In a preferred embodiment, the parts of the described spray form adding of step (3) fermented liquid are ram pump.The liquid flow rate of ram pump and lift will make bacterium liquid atomize fully evenly to spray.Certainly, also can be the device of other aerosolizable sprinklings.
Further, the particle diameter of described amino acid organic granular agent is controlled at 1.0-4.0mm.This particle diameter can make the infiltration of bacterium liquid evenly, and the specific surface area of appropriate particle size also makes the living bacteria count of postvaccinal granular organic fertilizer reach quality standards.
In a preferred embodiment, described step (2) can be added one or more of following material in the spray granulating operation: nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, trace element fertilizer.If produce the composite microbiological fertilizer of higher nutrient content, can in the spray granulating process, add nitrogen phosphorus potassium inorganic nutrients raw material, to improve total nutrient (N+P in the product
2O
5+ K
2O) content, its concrete prescription obtains according to the nutrient content of different material and the technical indicator cubage of product.Wherein the main raw material of N has: urea, ammonium sulfate; P
2O
5Main raw material have: monoammonium phosphate, diammonium phosphate, calcium superphosphate and calcium magnesium phosphorus; K
2The main raw material of O has: Repone K and vitriolate of tartar, trace element in also can adding: magnesium sal epsom; Calcium nitrocalcite; The manganese manganous sulfate; Zinc zinc sulfate; The boron borax; Copper copper sulfate, iron is with ferrous sulfate etc.
Further, described step (2) can be added organic matter material in the spray granulating operation.If produce the more composite microbiological fertilizer of high organic content, can in the spray granulating process, add organic matter material, to improve content organic in the product, concrete prescription obtains according to the content of organic matter material and the technical indicator cubage of product, and organic matter material has: the peat composed of rotten mosses, humic acids etc.
It is air-dry or dry that above-mentioned steps (3) is mentioned, that the composite microbiological fertilizer that will be sprayed with bacterium liquid is slightly done just to dry through the cooling cylinder that cold wind is arranged and can be entered feed bin, as finished product packing, if there is not cooling apparatus, less than 6% organic granular agent, evenly spraying just can become finished product after the inoculation of bacterium liquid and directly enter the feed bin packing and put in storage for moisture.
Because amino acid organic granular agent moisture content is very low, all in 10%, the bacterium liquid that sprays 0.5-1.5% can not impact moisture, the intensity of composite microbiological fertilizer particle, and can not surpass particle composite microbiological fertilizer moisture less than 20% requirement.
Adopt the compound method of liquid composite microbial microbial inoculum of the present invention and organic granular agent, according to physico-chemical properties such as the water content of amino acid organic granular agent and granularities, rationally control the adding proportion of liquid bacterial agent, not only make the composite microbiological fertilizer that makes meet the requirement of " composite microbiological fertilizer " agriculture standard (NY/T798-2004), and simplified the step of oven dry, directly put in storage or only need cold wind to dry slightly and can put in storage, it is fixed that the time that cold wind dries can come according to the speed of at that time moisture content, atmospheric moisture and the infiltration of bacterium liquid of organic granular.
A large amount of high pollution discharging of waste liquid is a difficult problem that perplexs for a long time monosodium glutamate (L-glutamic acid is the main raw material of monosodium glutamate) manufacturing enterprise in the L-glutamic acid production process, the waste liquid of medium-sized manufacturing enterprise generation every day reaches kiloton, these waste liquid acidity are very strong, if do not deal carefully with the severe acidification that can cause soil and water after the discharging, pollution to environment will seriously restrict the development of glutamate production enterprise, the compulsory execution of the pollution treatment cost of great number and the national industrial policies of low-carbon economy and energy-saving and emission-reduction, a lot of enterprises stop production and close down.Owing to contain a large amount of soluble nitrogens, phosphorus, potassium nutrition, organic matter, amino acid, humic acids and various trace elements in the waste liquid, in order properly to dispose these waste liquids, a lot of glutamate production enterprises are through technological improvement, waste liquid is produced the agent of amino acid organic granular through concentrated whitewashing, turning waste into wealth has both solved the pollution problem of waste liquid, has produced again organic granular agent material product.But because the agent of amino acid organic granular is with low content of technology, added value is not high, has affected the popularization of product, but L-glutamic acid and glutamate production enterprise cause large-tonnage product to overstock in order to dispose waste liquid and can not to stop to produce, and cause heavy burden to enterprise.
The present invention utilizes in L-glutamic acid and the glutamate production process and to produce the amino acid waste liquid and make the agent of amino acid organic granular through spray granulating, then microbiobacterial agent is inoculated into the way of amino acid organic granular agent to reach the purpose of producing composite microbiological fertilizer.The invention solves balling-up problem and the dissolution problems of composite microbiological fertilizer, enlarge the product use range, not only can be used for the cash crop such as Southern Fruit Trees, vegetables and do the base fertilizer use, the mechanical fertilization that also can be used for the north, make things convenient for the peasant to use, and because cost of material cheaply is easy to get, and product price is also relatively cheap, make the result of use of product fast and obvious.Total nutrient (N+P in the composite microbiological fertilizer that the present invention produces
2O
5+ K
2O) can reach 6%-25%, meet " composite microbe fertilizer " agriculture standard (NY/T798-2004) fully, effectively solve the technical problem that composite microbiological fertilizer faces at present, remedy deficiency of the prior art, the production method of soluble composite microorganism fertilizer a kind of cheapness, high, the environmental protection of resource utilization is provided, makes simultaneously and produce amino acid waste liquid that L-glutamic acid produces and reach disposal of resources and effectively utilize.
From having disclosed data now: the material that has is also reported the production method of granulated fertilizer dressing bacteria agent, but see it is at inorganic composite fertilizer (or composite fertilizer) surface coatings microbial inoculum from example, because compound manure belongs to the chemical fertilizer of high-content, in the combination of surface coatings microbial inoculum owing to inorganic nutrients and microorganism, a large amount of salt ions infiltrates thalline can cause the microorganism mortality, can't play the purpose of effective inoculation, science not at chemical fertilizer or inorganic composite fertilizer surface coatings microbial inoculum, irrational.The simple declaration that is reported in organic granular agent surface coatings microbial inoculum is also arranged, but composition and the content of organic granular agent are not described, because the microorganism that contains in the microbiobacterial agent is living microorganism, different organic raw material has a great impact its vital movement, the composition of concrete organic granular agent is not described, content and proterties, also can't realize the possibility of dressing bacterial classification success, so the data granulated fertilizer dressing bacterial classification of above report does not have feasibility, and these data do not illustrate the technological standard that obtains product after the dressing microbial inoculum yet, can't obtain the license of country and realize producing and promoting.
Compared with prior art, the present invention has following beneficial effect:
1, a kind of new starting material are provided for composite microbe fertilizer production; broken the traditional mode of producing composite microbiological fertilizer with humic acids or the peat composed of rotten mosses; for a kind of new starting material have been found in the production of bio-feritlizer; and solved the production technique problem of utilizing this raw material; granulating and the solubility problem of composite microbiological fertilizer have also been solved; in the production cost that reduces bio-feritlizer, quality product is improved.
2, solved the technical barrier that utilizes spray granulating to produce bio-feritlizer: spray granulating uniform particles, smooth surface, granule strength height, commodity is good, be easy to dissolve and be subject to user's welcome, but because the high temperature (above 1000 ℃) of spray granulating technique is dried and can't be carried out the production of microorganism particle fertilizer, can and access the way that bacterial classification separately carries out with the waste liquid spray granulating by this Technology, realize production and the application of composite microbiological fertilizer whitewashing particle.
3, safety, environmental protection.Present technique directly changes into the agent of amino acid organic granular and composite microbiological fertilizer with the waste liquid that produces in the L-glutamic acid production process; eliminated exhaust emission and because obtaining product good economic benefit has been arranged again; improvement is thorough, blowdown is zero; effluent resource; the engineering non-wastewater discharge; ecotope has been protected in fully reuse and not effluxing of the water of condensation that is produced by secondary steam.
4, solubilized, fertilizer efficiency is fast: produce composite microbiological fertilizer with the agent of amino acid organic granular, wherein contain a large amount of amino acid, solubility small molecules organic matter, micro-, amino acid can directly absorb for all parts of a plant, beneficial microorganism in the product can obtain a lot of nutrition from the agent of amino acid organic granular, Fast-propagation in soil namely can be observed positive effect in a short time after product is used.
5, enlarged the use range of product: with the composite microbiological fertilizer granulating, not only can be used for the vegetables fruit tree and make base fertilizer and use, also can be used for the land for growing field crops and carry out machine sowing, also use can be mixed with granular composite fertilizer, improved the market competitiveness of product.
6, raw material is easy to get, and technique is simple, can produce in enormous quantities, is not subjected to resource limit.
Description of drawings
Fig. 1 is amino acid organic granular agent output schema.
Fig. 2 is the agent of amino acid organic granular and liquid composite microbial microbial inoculum combined producing tech schema.
Embodiment
Embodiment 1
1, the amino acid waste liquid spray granulating in the glutamate production process is become the organic granular agent, the proterties of described organic granular agent sees Table 2.
Table 2: the basic proterties of amino acid organic granular agent
The preparation of liquid bacterial agent: with subtilis (Bacillus subtilis), bacillus megaterium (Bacillus megaterium) and Bacillus mucilaginosus (Bacillus mucilagimosus krassilm) utilize the independent fermentative production of fermentation equipment, three kinds of single bacterium liquid are mixed according to 2: 1: 1 ratio, make effective total viable count of the liquid composite microbial microbial inoculum that three bacterium liquid mix after mixing reach 45.2 * 10
8Individual/gram.
2, open stirrer, slowly evenly add the organic granular agent, continuous uniform is mobile forward to make material, in the stirrer that rotates, with ram pump the ratio spray form of bacterium liquid by weight 0.6% evenly is sprayed at organic granular agent surface (namely 100 kilograms organic granular agent in 0.6 kilogram of bacterium liquid of sprinkling), then the organic granular agent is air-dry or dry.
3, even postvaccinal organic granular agent is delivered directly to feed bin with conveyor and carries out packing, warehouse-in.
Embodiment 2
Amino acid waste liquid spray granulating with in the glutamate production process adds monoammonium phosphate, borax and humic acid in granulation process.
1, the preparation of liquid bacterial agent: subtilis (Bacillus subtilis) and bacillus megaterium (Bacillus megaterium) are utilized the independent fermentative production of fermentation equipment, two kinds of single bacterium liquid are mixed according to 1: 1 ratio, make effective total viable count of the liquid composite microbial microbial inoculum that two bacterium liquid mix after mixing reach 47.6 * 10
8Individual/gram.
2, open stirrer, slowly evenly add the organic granular agent, make material forward continuous uniform flow, in the stirrer that rotates, with ram pump the ratio of bacterium liquid by weight 0.8% evenly is sprayed at organic granular agent surface (namely 100 kilograms organic granular agent in 0.8 kilogram of bacterium liquid of sprinkling).
3, even postvaccinal organic granular agent is delivered directly to feed bin with conveyor and carries out packing, warehouse-in.
Embodiment 3
Amino acid waste liquid spray granulating with in the glutamate production process adds urea, vitriolate of tartar and ferrous sulfate in granulation process.
1, the preparation of liquid bacterial agent: subtilis (Bacillus subtilis) and Bacillus mucilaginosus (Bacillus mucilagimosus krassilm) are utilized the independent fermentative production of fermentation equipment, two kinds of single bacterium liquid are mixed according to 2: 1 ratio, make effective total viable count of the liquid composite microbial microbial inoculum that two bacterium liquid mix after mixing reach 41.6 * 10
8Individual/gram.
2, open stirrer, slowly evenly add the organic granular agent, make material forward continuous uniform flow, in the stirrer that rotates, with ram pump the ratio of bacterium liquid by weight 0.8% evenly is sprayed at organic granular agent surface (namely 100 kilograms organic granular agent in 0.8 kilogram of bacterium liquid of sprinkling).
3, even postvaccinal organic granular agent is delivered directly to feed bin with conveyor and carries out packing, warehouse-in.
Embodiment 4
Amino acid waste liquid spray granulating with in the glutamate production process adds ammonium sulfate, ammonium phosphate and sal epsom in granulation process.
1, the preparation of liquid bacterial agent: subtilis (Bacillus subtilis) and Bacillus mucilaginosus (Bacillus mucilagimosus krassilm) are utilized the independent fermentative production of fermentation equipment, two kinds of single bacterium liquid are mixed according to 1.5: 1 ratio, and effective total viable count of the liquid composite microbial microbial inoculum that two bacterium liquid mix after mixing reaches 42.6 * 10
8Individual/gram.
2, open stirrer, slowly evenly add the organic granular agent, make material forward continuous uniform flow, in the stirrer that rotates, with ram pump the ratio of bacterium liquid by weight 1% evenly is sprayed at organic granular agent surface (namely 100 kilograms organic granular agent in 1 kilogram of bacterium liquid of sprinkling).
3, even postvaccinal organic granular agent is delivered directly to feed bin with conveyor and carries out packing, warehouse-in.
Embodiment 5
Amino acid waste liquid spray granulating with in the glutamate production process adds ammonium sulfate, urea, monoammonium phosphate, manganous sulfate and Sodium orthomolybdate in granulation process.
1, the preparation of liquid bacterial agent: bacillus megaterium (Bacillus megaterium) and Bacillus mucilaginosus (Bacillus mucilagimosus krassilm) are utilized the independent fermentative production of fermentation equipment, two kinds of single bacterium liquid are mixed according to 2: 1 ratio, and effective total viable count of the liquid composite microbial microbial inoculum that two bacterium liquid mix after mixing reaches 39.2 * 10
8Individual/gram.
2, open stirrer, slowly evenly add the organic granular agent, make material forward continuous uniform flow, in the stirrer that rotates, with ram pump the ratio of bacterium liquid by weight 1.1% evenly is sprayed at organic granular agent surface (namely 100 kilograms organic granular agent in 1.1 kilograms of bacterium liquid of sprinkling).
3, even postvaccinal organic granular agent is delivered directly to feed bin with conveyor and carries out packing, warehouse-in.
Embodiment 6
Amino acid waste liquid spray granulating with in the glutamate production process adds ammonium sulfate, monoammonium phosphate, Repone K, zinc sulfate and the peat composed of rotten mosses in granulation process.
1, the preparation of liquid bacterial agent: bacillus megaterium (Bacillus megaterium) is utilized the independent fermentative production of fermentation equipment, and effective total viable count of the liquid microbe microbial inoculum of bacterium liquid reaches 41 * 10 after the fermentation
8Individual/gram.
2, open stirrer, slowly evenly add the organic granular agent, make material forward continuous uniform flow, in the stirrer that rotates, with ram pump the ratio of bacterium liquid by weight 0.9% evenly is sprayed at organic granular agent surface (namely 100 kilograms organic granular agent in 0.9 kilogram of bacterium liquid of sprinkling).
3, even postvaccinal organic granular agent is delivered directly to feed bin with conveyor and carries out packing, warehouse-in.
Embodiment 7
Amino acid waste liquid spray granulating with in the glutamate production process adds ammonium sulfate, diammonium phosphate in granulation process.
1, the preparation of liquid bacterial agent: subtilis (Bacillus subtilis) is utilized the independent fermentative production of fermentation equipment, and effective total viable count of the liquid microbe microbial inoculum of bacterium liquid reaches 49.6 * 10 after the fermentation
8Individual/gram.
2, open stirrer, slowly evenly add the organic granular agent, make material forward continuous uniform flow, in the stirrer that rotates, with ram pump the ratio of bacterium liquid by weight 0.5% evenly is sprayed at organic granular agent surface (namely 100 kilograms organic granular agent in 0.5 kilogram of bacterium liquid of sprinkling).
3, even postvaccinal organic granular agent is delivered directly to feed bin with conveyor and carries out packing, warehouse-in.
Embodiment 8
Amino acid waste liquid spray granulating with in the glutamate production process adds ammonium sulfate, diammonium phosphate and humic acid in granulation process.
1, the preparation of liquid bacterial agent: Bacillus mucilaginosus (Bacillus mucilagimosus krassilm) is utilized the independent fermentative production of fermentation equipment, and effective total viable count of the liquid microbe microbial inoculum of bacterium liquid reaches 25.3 * 10 after the fermentation
8Individual/gram.
2, open stirrer, slowly evenly add the organic granular agent, make material forward continuous uniform flow, in the stirrer that rotates, with ram pump the ratio of bacterium liquid by weight 1.2% evenly is sprayed at organic granular agent surface (namely 100 kilograms organic granular agent in 1.2 kilograms of bacterium liquid of sprinkling).
3, even postvaccinal organic granular agent is delivered directly to feed bin with conveyor and carries out packing, warehouse-in.
Embodiment 9
Amino acid waste liquid spray granulating with in the glutamate production process adds urea, diammonium phosphate and copper sulfate in granulation process.
1, the preparation of liquid bacterial agent: with subtilis (Bacillus subtilis), bacillus megaterium (Bacillus megaterium) and Bacillus mucilaginosus (Bacillus mucilagimosus krassilm) utilize the independent fermentative production of fermentation equipment, three kinds of single bacterium liquid are mixed according to 2.5: 1.5: 1.5 ratio, make effective total viable count of the liquid composite microbial microbial inoculum that three bacterium liquid mix after mixing reach 46.2 * 10
8Individual/gram.
2, open stirrer, slowly evenly add the organic granular agent, make material forward continuous uniform flow, in the stirrer that rotates, with ram pump the ratio spray form of bacterium liquid by weight 0.6% evenly is sprayed at organic granular agent surface (namely 100 kilograms organic granular agent in 0.6 kilogram of bacterium liquid of sprinkling).
3, even postvaccinal organic granular agent is delivered directly to feed bin with conveyor and carries out packing, warehouse-in.
Comparative example 1
Produce composite microbiological fertilizer with humic acids as organic materials
1, humic acid sampling.Come from Linfen, Shanxi, basic proterties sees Table 3
Table 3: the basic proterties of humic acids
| Project | Organic (%) | pH | Fineness (order) | Humic acids (%) | Moisture (%) | Miscellaneous bacteria rate (%) |
| Content | 51.6 | 5.7 | 100 | 40.3 | 20.6 | 16.4 |
The concrete preparation of complex micro organism fungicide and content's index are with embodiment 1.
2, batching: the formula table of pressing table 4 adds various materials in the stirrer, then stirs.
Table 4: the production formula of composite microbiological fertilizer
3, granulation: with carrying out granulation in the solid materials adding granulating disc that stirs, in granulation process, evenly bacterium liquid is sparged in the disk, make the diameter of most of grain at 2-4cm.
4, air-dry or dry, be delivered directly to feed bin with conveyor and carry out packing, warehouse-in.
Product quality inspection
The examination and test of products is carried out according to the method for Ministry of Agriculture's industry standard (NY/T798-2004), with the agent of amino acid organic granular as organic raw material, produce particle composite microbial material product with the liquid composite microbial microbial inoculum, and humic acids produces composite microbiological fertilizer as organic materials and preserves simultaneously and detect after 10 days, the results are shown in Table 5:
Table 5: the composite microbe fertilizer quality product detected result that different embodiment produce
Can find out from detected result: all reach about 2,000 ten thousand/gram N+P in its living bacteria count quantity of the composite microbiological fertilizer product of producing behind the Mixed inoculation microbiobacterial agent in the agent of amino acid organic granular
2O
5+ K
2O is higher than 6%, granule strength is greater than 7N, granularity is all more than 90%, miscellaneous bacteria is no more than 9.5%, and moisture is lower than 8.5%, and indices is all considerably beyond the requirement of Ministry of Agriculture's industry standard, and the composite microbiological fertilizer moisture of producing with the peat composed of rotten mosses is high, the miscellaneous bacteria rate is high, and granule strength and granularity are low, and the commodity performance is starkly lower than the product that the agent of amino acid organic granular is produced.
Test effect:
Test is established three and is processed as follows respectively:
A: 100 kg/acres of amino acid composite microbe fertilizers (agent of amino acid organic granular and liquid composite microbial microbial inoculum are compound);
B: 100 kg/acres of composite microbe fertilizers (peat composed of rotten mosses and the granulation of liquid composite microbial microbial inoculum complex disc).
Residential quarter area 20m
2, establish 3 repetitions, district's group is arranged at random.The trial crops tomato is tested fertile sample technical indicator mensuration and sees Table 5, and all tests are processed and all made base manure, and manuring late and management are identical with habits fertilization.Data statistical approach: yield data is checked significance with duncan's new multiple range method.
Table 6: different treatment on tomato trunk blade, highly, the impact of climing transverse diameter
Table 7: different treatment is on the impact of tomato yield
Table 6 can be found out: the number of blade, the plant height transverse diameter that three different treatment are processed tomato growth A all is significantly higher than B and processes, and illustrates that the amino acid composite microbe fertilizer will get well the common composite microbiological fertilizer of the growth fraction of tomato.Table 7 can obviously be found out: the output that A processes than B processing tomato improves far away, illustrate and use this amino acid micro organism composite fertilizer more to be conducive to improve tomato growth and output than with common micro-organisms, amino acid composite biological fertilizer all more common composite microbe fertilizer effect of increasing production has reached conspicuous level.
Claims (6)
1. a method of utilizing the agent of amino acid organic granular to produce composite microbiological fertilizer comprises the preparation of microbial inoculum, and it is characterized in that: the method may further comprise the steps:
(1) liquid bacterial agent is the preparation of fermented liquid: will utilize for the microbial bacteria of fertilizer field the fermentation equipment fermentative production, and obtain fermented liquid, total living bacteria count reaches 25-60 hundred million/gram in the described fermented liquid;
(2) granulation: the amino acid waste liquid is through the agent of spray granulating granulation amino acid organic granular;
(3) amino acid organic granular agent inoculation: in the stirrer that amino acid organic granular agent adding material continuous uniform is flowed, add the fermented liquid that step (1) obtains with spray form, then air-dry or dry, obtain the composite microbiological fertilizer of finished product;
Described fermented liquid add-on accounts for the 0.5%-1.5% of finished product composite microbiological fertilizer gross weight; Described amino acid waste liquid is the by product in L-glutamic acid and the glutamate production process.
2. the described method of utilizing the agent of amino acid organic granular to produce composite microbiological fertilizer according to claim 1, it is characterized in that: described microbial bacteria for fertilizer field is selected from subtilis (Bacillus subtilis), one or more in bacillus megaterium (Bacillus megaterium) and the Bacillus mucilaginosus (Bacillus mucilagimosus krassilm).
3. the described method of utilizing the agent of amino acid organic granular to produce composite microbiological fertilizer according to claim 1, it is characterized in that: the described stirrer of step (3) is disk or rotary drum cylinder.
4. the described method of utilizing the agent of amino acid organic granular to produce composite microbiological fertilizer according to claim 1, it is characterized in that: the parts that the described spray form of step (3) adds fermented liquid are ram pump.
5. the described method of utilizing the agent of amino acid organic granular to produce composite microbiological fertilizer according to claim 1, it is characterized in that: described step (2) is added one or more of following material in the spray granulating operation: nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, trace element fertilizer.
6. the described method of utilizing the agent of amino acid organic granular to produce composite microbiological fertilizer according to claim 1, it is characterized in that: described step (2) is added organic matter material in the spray granulating operation.
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| CN102765983A (en) * | 2012-07-16 | 2012-11-07 | 江阴市联业生物科技有限公司 | Organic and inorganic compound fertilizer |
| CN104446817A (en) * | 2014-12-16 | 2015-03-25 | 东北农业大学 | Special fertilizer for herbal wild vegetable seedling and use method of fertilizer |
| CN105036974B (en) * | 2015-08-07 | 2018-06-29 | 沃达农业科技股份有限公司 | Amino acid microbial ecological fertilizer and its preparation and application |
| CN108101707B (en) * | 2017-12-31 | 2021-04-06 | 三门峡龙飞生物工程有限公司 | Total nutrient microbial amino acid water-soluble fertilizer and preparation method thereof |
| CN112159274A (en) * | 2020-09-28 | 2021-01-01 | 山东土木启生物科技有限公司 | Microbial granular inoculant with biocontrol nutrition function |
| CN113896579A (en) * | 2021-09-18 | 2022-01-07 | 贵州省贵福生态肥业有限公司 | Special carbon-based microbial fertilizer deep fermentation device for tea and use method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1149569A (en) * | 1995-10-30 | 1997-05-14 | 唐增义 | Amino acid granular compound fertilizer and its producing method |
| CN1478762A (en) * | 2003-07-01 | 2004-03-03 | 中化国际化肥贸易公司 | Blending organic-inorganic composite fertilizer |
| CN101468927A (en) * | 2007-12-27 | 2009-07-01 | 袁洋 | Biological organic inorganic compound fertilizer product and production method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1149569A (en) * | 1995-10-30 | 1997-05-14 | 唐增义 | Amino acid granular compound fertilizer and its producing method |
| CN1478762A (en) * | 2003-07-01 | 2004-03-03 | 中化国际化肥贸易公司 | Blending organic-inorganic composite fertilizer |
| CN101468927A (en) * | 2007-12-27 | 2009-07-01 | 袁洋 | Biological organic inorganic compound fertilizer product and production method thereof |
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