CN102028755B - Preparation method of traditional Chinese medicine aerosol for treating respiratory system diseases of birds - Google Patents
Preparation method of traditional Chinese medicine aerosol for treating respiratory system diseases of birds Download PDFInfo
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Abstract
The invention provides a preparation method of traditional Chinese medicine aerosol for treating respiratory system diseases of birds, which comprises the specific steps of: 1, soaking astragalus root, the fruit of Chinese magnoliavine, officinal magnolia bark and liquorice root with water and then carrying out reflux extraction at normal pressure, filtering out a solution and condensing; 2, condensing residue of medicinal materials with 80 percent (V/V) alcohol; 3, mixing condensate, extracting and filtering, vacuum drying; 4, after crushing bee glue, extracting the crushed bee glue with absolute ethyl alcohol; 5, collecting bee glue extract, condensing recycled liquid, decompressing and drying; 6, adding traditional Chinese medicine powder prepared in the step 3 and the step 5 into deionized water or pure water to obtain a traditional Chinese medicine premixed solution; 7, under the condition of an anti-explosion environment, adding the absolute ethyl alcohol in a tank with a cover; 8, continuously adding 99.6 percent glycerol; and 9, filtering with a filter, canning with a canning machine, and injecting a propellent after sterilizing and covering. The preparation method is simple, and is suitable for the requirement for the massive industrialized production.
Description
Technical field
The present invention relates to a kind of method for preparing of veterinary drug, especially a kind of method for preparing that is used to treat the medicinal herbs aerosol inhalation agent of fowl respiratory system disease.
Background technology
Respiratory tract disease is by the growth that has a strong impact on birds that the factor combined effect causes and the accompanying infection property disease of fertility performance such as infectious, malnutrition, feed management be bad, causes the birds fertility performance low, and economic loss is serious.Antibiotic is the choice drug of such respiratory tract system disease of treatment; But because the residue problem of antibiotic drug resistance and medicine becomes increasingly conspicuous; In recent years domesticly also developed a series of Chinese herbal and crude drugs preparationses, respiratory disease of birds has been had curative effect preferably, but the at present administrations such as spice is fed, oral, intramuscular injection of taking of the treatment of respiratory tract system disease more; Oral administration dosage is big, and medicine is prone to be destroyed at gastrointestinal tract; The intramuscular injection drug bioavailability is low, and some is difficult to influence drug effect through " blood gas barrier ", and birds air bag position blood flow is few, oral administration or the extremely difficult onset of injection.Therefore research and develop a kind of Chinese medicine composition through effective route of administration treatment fowl respiratory system disease, have very important production practices meaning.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of method for preparing that is used to treat the medicinal herbs aerosol inhalation agent of fowl respiratory system disease.
For solving the problems of the technologies described above, technical scheme of the present invention is:
A kind of method for preparing that is used to treat the medicinal herbs aerosol inhalation agent of fowl respiratory system disease, concrete steps are:
(1) Radix Astragali, Fructus Schisandrae Chinensis, Cortex Magnoliae Officinalis and the Radix Glycyrrhizae back atmospheric pressure reflux that is soaked in water is extracted, leached solution and concentrate, concentrated solution being got filtrating concentrating as for staticly settling in 80% (V/V) ethanol, and medical material weight is 1 with concentrated solution volume ratio: 1-1.2 (g/ml);
(2) with after adding 80% (V/V) ethanol mixing in the above-mentioned medical material residue, room temperature leaves standstill filtration, concentrated filtrate, and medical material weight is 1 with concentrated solution volume ratio: 0.6-1.2 (g/ml);
(3) concentrated solution that step (1) and step (2) is obtained merges, sucking filtration, and vacuum drying, powder for drying is subsequent use;
(4) propolis was pulverized the back with dehydrated alcohol extraction 12-24 hour, medical material weight and dehydrated alcohol volume ratio are 1: 3-6 (g/ml);
(5) collect propolis extract, with the quiet filter made of diatomite filter, concentrate recovery liquid drying under reduced pressure, pulverize after being cooled to room temperature, powder for drying is subsequent use;
(6) Chinese medicinal powder that makes in step (3) and the step (5) is joined in deionized water or the purified water, this Chinese medicinal powder weight and deionized water or purified water volume ratio are 1: 1-2 (g/ml), stir, and get the Chinese medicine aqueous premix;
(7) under the explosion-proof environment condition, in jar with cover, add dehydrated alcohol, slowly stir then, slowly add the Chinese medicine aqueous premix that makes in the step (6), by its volume parts dehydrated alcohol 85.9-92.2 part, Chinese medicine aqueous premix 1.65-4.4 part;
(8) continue slowly to add glycerol 6.1-11.9 part, stir until obtaining limpid solution by its volume parts 99.6%;
(9) filter filters, and the filling machine fill is poured propellant behind the sterilization gland;
Wherein, the above-mentioned Radix Astragali, propolis, Fructus Schisandrae Chinensis, Cortex Magnoliae Officinalis and Radix Glycyrrhizae are got Radix Astragali 44.4-64.5 part, propolis 3.45-6.45 part, Fructus Schisandrae Chinensis 6.67-12.5 part, Cortex Magnoliae Officinalis 19.4-20.8 part, Radix Glycyrrhizae 34.5-44.5 part by its parts by weight.
Preferably, the above-mentioned method for preparing that is used to treat the medicinal herbs aerosol inhalation agent of fowl respiratory system disease, concrete steps are:
(1) Radix Astragali, Fructus Schisandrae Chinensis, Cortex Magnoliae Officinalis and Radix Glycyrrhizae are added the water logging that accounts for 8 times of amounts of its quality and steeped 2 hours, atmospheric pressure reflux was extracted 2 hours, leached solution; Add the water atmospheric pressure reflux 2 hours account for 5 times of amounts of its quality in the medical material again, leach filtrating; Merge filtrating and concentrated twice, medical material weight is 1 with concentrated solution volume ratio: 1-2 (g/ml), in concentrated solution 80% (V/V) ethanol, staticly settled 24 hours as for 3 times of volumes, and filter; Filtrate recycling ethanol, concentrated filtrate, medical material weight is 1 with concentrated solution volume ratio: 1-1.2 (g/ml);
(2) with add in the above-mentioned medical material residue account for 80% (V/V) ethanol mixing of 8 times of amounts of its quality after, room temperature left standstill 24 hours, filtered; Filtrate recycling ethanol, concentrated filtrate, medical material weight is 1 with concentrated solution volume ratio: 0.6-1.2 (g/ml);
(3) concentrated solution that step (1) and step (2) is obtained merges, sucking filtration, and vacuum drying, powder for drying is subsequent use, the powder diameter range: 0.5-10 μ m;
(4) propolis powder is broken into 60-80 order powder, with dehydrated alcohol extraction 12-24 hour, medical material weight and dehydrated alcohol volume ratio were 1: 3-6 (g/ml);
(5) collect propolis extract, with the quiet filter made of diatomite filter, concentrate recovery liquid drying under reduced pressure, pulverize after being cooled to room temperature, powder for drying is subsequent use; Particle size range: 0.5-10 μ m;
(6) Chinese medicinal powder that makes in step (3) and the step (5) is joined in deionized water or the purified water, this Chinese medicinal powder weight and deionized water or purified water volume ratio are 1: 1-2 (g/ml), stir, and get the Chinese medicine aqueous premix;
(7) under the explosion-proof environment condition, in jar with cover, add dehydrated alcohol, slowly stir then, slowly add the Chinese medicine aqueous premix that makes in the step (6), by its volume parts dehydrated alcohol 85.9-92.2 part, Chinese medicine aqueous premix 1.65-4.4 part;
(8) continue slowly to add glycerol 6.1-11.9 part, stir until obtaining limpid solution by its volume parts 99.6%;
(9) with 2-10 μ m, and can tolerate alcoholic acid filer element type filter filtration, the filling machine fill is poured propellant behind the sterilization gland;
Wherein, the above-mentioned Radix Astragali, propolis, Fructus Schisandrae Chinensis, Cortex Magnoliae Officinalis and Radix Glycyrrhizae are got Radix Astragali 44.4-64.5 part, propolis 3.45-6.45 part, Fructus Schisandrae Chinensis 6.67-12.5 part, Cortex Magnoliae Officinalis 19.4-20.8 part, Radix Glycyrrhizae 34.5-44.5 part by its parts by weight.
Above-mentioned dehydrated alcohol refers to the ethanol of 95%-100% (V/V).
Preferably, the above-mentioned method for preparing that is used to treat the medicinal herbs aerosol inhalation agent of fowl respiratory system disease, said propellant is one or more in tetrafluoroethane (HFA134a), heptafluoro-propane (HFA227) or the iso-butane.
Preferably; The above-mentioned method for preparing that is used to treat the medicinal herbs aerosol inhalation agent of fowl respiratory system disease; Said cosolvent is dehydrated alcohol, 99.6% glycerol and deionized water or purified water, by its volume parts: dehydrated alcohol 85.9-92.2 part, glycerol 6.1-11.9 part, deionized water or purified water 1.6-2.2 part.
Preferably, the above-mentioned method for preparing that is used to treat the medicinal herbs aerosol inhalation agent of fowl respiratory system disease is got 50 parts of the Radixs Astragali, 5 parts of propolis, 12 parts of Fructus Schisandrae Chinensis, 20 parts of Cortex Magnoliae Officinalis, 40 parts in Radix Glycyrrhizae by its parts by weight.
Preferably, the above-mentioned method for preparing that is used to treat the medicinal herbs aerosol inhalation agent of fowl respiratory system disease is got 64.4 parts of the Radixs Astragali, 6.4 parts of propolis, 6.68 parts of Fructus Schisandrae Chinensis, 19.4 parts of Cortex Magnoliae Officinalis, 34.6 parts in Radix Glycyrrhizae by its parts by weight.
Preferably, the above-mentioned method for preparing that is used to treat the medicinal herbs aerosol inhalation agent of fowl respiratory system disease is got 44.4 parts of the Radixs Astragali, 3.5 parts of propolis, 12.4 parts of Fructus Schisandrae Chinensis, 20.8 parts of Cortex Magnoliae Officinalis, 44.4 parts in Radix Glycyrrhizae by its parts by weight.
Preferably, the above-mentioned method for preparing that is used to treat the medicinal herbs aerosol inhalation agent of fowl respiratory system disease is got 55 parts of the Radixs Astragali, 5.6 parts of propolis, 9.6 parts of Fructus Schisandrae Chinensis, 20.4 parts of Cortex Magnoliae Officinalis, 38 parts in Radix Glycyrrhizae by its parts by weight.
Preferably, the above-mentioned method for preparing that is used to treat the medicinal herbs aerosol inhalation agent of fowl respiratory system disease is got 48 parts of the Radixs Astragali, 4 parts of propolis, 8 parts of Fructus Schisandrae Chinensis, 19.6 parts of Cortex Magnoliae Officinalis, 42 parts in Radix Glycyrrhizae by its parts by weight.
Preferably, the above-mentioned method for preparing that is used to treat the medicinal herbs aerosol inhalation agent of fowl respiratory system disease is got 60 parts of the Radixs Astragali, 6 parts of propolis, 11 parts of Fructus Schisandrae Chinensis, 20.2 parts of Cortex Magnoliae Officinalis, 37 parts in Radix Glycyrrhizae by its parts by weight.
The consumption that being used to of method for preparing treated the medicinal herbs aerosol inhalation agent of fowl respiratory system disease is 20mL/d.
The invention has the beneficial effects as follows:
The above-mentioned method for preparing that is used to treat the medicinal herbs aerosol inhalation agent of fowl respiratory system disease, simple to operate, prepared aerosol drug absorbs rapidly; Through focus, and keep higher drug level at inflammation part, particularly at organs such as nasal cavity, trachea, bronchus, pulmonary, air bags; Can thoroughly kill the pathogen of air bag position, pulmonary, alleviate respiratory symptom, instant effect; Administration number of times is few, and is easy to use, practical; Be fit to requirements of large-scale industrial production.
The specific embodiment
Below in conjunction with specific embodiment technical scheme according to the invention is further described.
Embodiment 1
(1) Radix Astragali 250g, Fructus Schisandrae Chinensis 60g, Cortex Magnoliae Officinalis 100g and Radix Glycyrrhizae 200g are added the water logging that accounts for 8 times of amounts of its quality and steeped 2 hours, atmospheric pressure reflux was extracted 2 hours, leached solution; Add the water atmospheric pressure reflux 2 hours account for 5 times of amounts of its quality in the medical material again, leach filtrating; Merge filtrating and concentrated twice, the concentrated medicament volume is 900ml, in concentrated solution 80% (V/V) ethanol as for 3 times of volumes, staticly settles 24 hours, filters; Filtrate recycling ethanol, concentrated filtrate, concentrated medicament volume are 730ml;
(2) with add in the above-mentioned medical material residue account for 80% (V/V) ethanol mixing of 8 times of amounts of its quality after, room temperature left standstill 24 hours, filtered; Filtrate recycling ethanol, concentrated filtrate, concentrated medicament volume are 610ml;
(3) concentrated solution that step (1) and step (2) is obtained merges, sucking filtration, vacuum drying, Chinese medicinal powder 60.5g, powder for drying is subsequent use, powder diameter 5 μ m;
(4) the 25g propolis powder is broken into 60 order powder, with the ethanol extraction of 150mL 95% (V/V) 24 hours;
(5) collect propolis extract,, concentrate and reclaim the liquid drying under reduced pressure, pulverize after being cooled to room temperature with the quiet filter made of diatomite filter, must powder 6.12g, powder for drying is subsequent use; Particle diameter 5 μ m;
(6) Chinese medicinal powder that makes in step (3) and the step (5) is joined in the 100ml deionized water, stir, get the Chinese medicine aqueous premix;
(7) under the explosion-proof environment condition, in jar with cover, add the 4000ml dehydrated alcohol, slowly stir then, slowly add the aqueous premix that makes in the step (6);
(8) continuation slowly adds 99.6% glycerol 300ml, stirs until obtaining limpid solution;
(9) with 6 μ m, and can tolerate alcoholic acid filer element type filter it is filtered, and be sent to filling machine; In filling machine fill to 100m l slush moulding vial; Behind the sterilization gland, temperature is controlled at 25 ℃, pours iso-butane through last valve; Net volume after valve is packaged is 85.4mL, and irritating has tcm product 1.13g (about 74.7mL).
Embodiment 2
(1) Radix Astragali 322g, Fructus Schisandrae Chinensis 33.4g, Cortex Magnoliae Officinalis 97g and Radix Glycyrrhizae 173g are added the water logging that accounts for 8 times of amounts of its quality and steeped 2 hours, atmospheric pressure reflux was extracted 2 hours, leached solution; Add the water atmospheric pressure reflux 2 hours account for 5 times of amounts of its quality in the medical material again, leach filtrating; Merge filtrating and concentrated twice, the concentrated medicament volume is 660ml, in concentrated solution 80% (V/V) ethanol as for 3 times of volumes, staticly settles 24 hours, filters; Filtrate recycling ethanol, concentrated filtrate, concentrated medicament volume are 660ml;
(2) with add in the above-mentioned medical material residue account for 80% (V/V) ethanol mixing of 8 times of amounts of its quality after, room temperature left standstill 24 hours, filtered; Filtrate recycling ethanol, concentrated filtrate, concentrated medicament volume are 650ml;
(3) concentrated solution that step (1) and step (2) is obtained merges, sucking filtration, vacuum drying, Chinese medicinal powder 59.54g, powder for drying is subsequent use, powder diameter 0.5 μ m;
(4) the 32g propolis powder is broken into 80 order powder, with the ethanol extraction of 100ml 97% (V/V) 12 hours;
(5) collect propolis extract,, concentrate and reclaim the liquid drying under reduced pressure, pulverize after being cooled to room temperature with the quiet filter made of diatomite filter, must powder 7.85g, powder for drying is subsequent use; Particle diameter 0.5 μ m;
(6) Chinese medicinal powder that makes in step (3) and the step (5) is joined in the 85m l purified water, stir, get the Chinese medicine aqueous premix;
(7) under the explosion-proof environment condition, in jar with cover, add the 4060ml dehydrated alcohol, slowly stir then, slowly add the aqueous premix that makes in the step (6);
(8) continuation slowly adds 99.6% glycerol 288ml, stirs until obtaining limpid solution;
(9) with 2 μ m, and can tolerate alcoholic acid filer element type filter it is filtered, and be sent to filling machine; In filling machine fill to 100ml slush moulding vial; Behind the sterilization gland, temperature is controlled at 25 ℃, pours tetrafluoroethane through last valve; Net volume after valve is packaged is 84.7mL, and irritating has tcm product 1.12g (about 73.5mL).
Embodiment 3
(1) Radix Astragali 222g, Fructus Schisandrae Chinensis 62g, Cortex Magnoliae Officinalis 104g and Radix Glycyrrhizae 222g are added the water logging that accounts for 8 times of amounts of its quality and steeped 2 hours, atmospheric pressure reflux was extracted 2 hours, leached solution; Add the water atmospheric pressure reflux 2 hours account for 5 times of amounts of its quality in the medical material again, leach filtrating; Merge filtrating and concentrated twice, the concentrated medicament volume is 1255ml, in concentrated solution 80% (V/V) ethanol as for 3 times of volumes, staticly settles 24 hours, filters; Filtrate recycling ethanol, concentrated filtrate, concentrated medicament volume are 630ml;
(2) with add in the above-mentioned medical material residue account for 80% (V/V) ethanol mixing of 8 times of amounts of its quality after, room temperature left standstill 24 hours, filtered; Filtrate recycling ethanol, concentrated filtrate, concentrated medicament volume are 650ml;
(3) concentrated solution that step (1) and step (2) is obtained merges, sucking filtration, vacuum drying, Chinese medicinal powder 63.81g, powder for drying is subsequent use, powder diameter 10 μ m;
(4) the 17.5g propolis powder is broken into 70 order powder, with the ethanol extraction of 70ml 99% (V/V) 18 hours;
(5) collect propolis extract,, concentrate and reclaim the liquid drying under reduced pressure, pulverize after being cooled to room temperature with the quiet filter made of diatomite filter, must powder 4.29g, powder for drying is subsequent use; Particle diameter 10 μ m;
(6) Chinese medicinal powder that makes in step (3) and the step (5) is joined in the 70ml deionized water, stir, get the Chinese medicine aqueous premix;
(7) under the explosion-proof environment condition, in jar with cover, add the 3760ml dehydrated alcohol, slowly stir then, slowly add the aqueous premix that makes in the step (6);
(8) continuation slowly adds 99.6% glycerol 270ml, stirs until obtaining limpid solution;
(9) with 10 μ m, and can tolerate alcoholic acid filer element type filter it is filtered, and be sent to filling machine; In filling machine fill to 100ml slush moulding vial; Behind the sterilization gland, temperature is controlled at 25 ℃, pours heptafluoro-propane through last valve; Net volume after valve is packaged is 86.5mL, and irritating has tcm product 1.19g (about 71.7mL).
Embodiment 4
(1) Radix Astragali 275g, Fructus Schisandrae Chinensis 48g, Cortex Magnoliae Officinalis 102g and Radix Glycyrrhizae 190g are added the water logging that accounts for 8 times of amounts of its quality and steeped 2 hours, atmospheric pressure reflux was extracted 2 hours, leached solution; Add the water atmospheric pressure reflux 2 hours account for 5 times of amounts of its quality in the medical material again, leach filtrating; Merge filtrating and concentrated twice, the concentrated medicament volume is 925ml, in concentrated solution 80% (V/V) ethanol as for 3 times of volumes, staticly settles 24 hours, filters; Filtrate recycling ethanol, concentrated filtrate, concentrated medicament volume are 675ml;
(2) with add in the above-mentioned medical material residue account for 80% (V/V) ethanol mixing of 8 times of amounts of its quality after, room temperature left standstill 24 hours, filtered; Filtrate recycling ethanol, concentrated filtrate, concentrated medicament volume are 700ml;
(3) concentrated solution that step (1) and step (2) is obtained merges, sucking filtration, vacuum drying, Chinese medicinal powder 68.89g, powder for drying is subsequent use, powder diameter 3 μ m;
(4) the 28g propolis powder is broken into 70 order powder, with the ethanol extraction of 140ml 95% (V/V) 22 hours;
(5) collect propolis extract,, concentrate and reclaim the liquid drying under reduced pressure, pulverize after being cooled to room temperature with the quiet filter made of diatomite filter, must powder 6.88g, powder for drying is subsequent use; Particle diameter 3 μ m;
(6) Chinese medicinal powder that makes in step (3) and the step (5) is joined in the 100ml deionized water, stir, get the Chinese medicine aqueous premix;
(7) under the explosion-proof environment condition, in jar with cover, add the 4240ml dehydrated alcohol, slowly stir then, slowly add the aqueous premix that makes in the step (6);
(8) continuation slowly adds 99.6% glycerol 524ml, stirs until obtaining limpid solution;
(9) with 4 μ m, and can tolerate alcoholic acid filer element type filter it is filtered, and be sent to filling machine; In filling machine fill to 100ml slush moulding vial; Behind the sterilization gland, temperature is controlled at 25 ℃, pours iso-butane through last valve; Net volume after valve is packaged is 85.0mL, and irritating has tcm product 1.13g (about 72.5mL).
Embodiment 5
(1) Radix Astragali 240g, Fructus Schisandrae Chinensis 40g, Cortex Magnoliae Officinalis 98g and Radix Glycyrrhizae 210g are added the water logging that accounts for 8 times of amounts of its quality and steeped 2 hours, atmospheric pressure reflux was extracted 2 hours, leached solution; Add the water atmospheric pressure reflux 2 hours account for 5 times of amounts of its quality in the medical material again, leach filtrating; Merge filtrating and concentrated twice, the concentrated medicament volume is 705ml, in concentrated solution 80% (V/V) ethanol as for 3 times of volumes, staticly settles 24 hours, filters; Filtrate recycling ethanol, concentrated filtrate, concentrated medicament volume are 705ml;
(2) with add in the above-mentioned medical material residue account for 80% (V/V) ethanol mixing of 8 times of amounts of its quality after, room temperature left standstill 24 hours, filtered; Filtrate recycling ethanol, concentrated filtrate, concentrated medicament volume are 500ml;
(3) concentrated solution that step (1) and step (2) is obtained merges, sucking filtration, vacuum drying, Chinese medicinal powder 61.25g, powder for drying is subsequent use, powder diameter 7 μ m;
(4) the 20g propolis powder is broken into 80 order powder, with the ethanol extraction of 70ml 96% (V/V) 14 hours;
(5) collect propolis extract,, concentrate and reclaim the liquid drying under reduced pressure, pulverize after being cooled to room temperature with the quiet filter made of diatomite filter, must powder 3.91g, powder for drying is subsequent use; Particle diameter 8 μ m;
(6) Chinese medicinal powder that makes in step (3) and the step (5) is joined in the 80ml purified water, stir, get the Chinese medicine aqueous premix;
(7) under the explosion-proof environment condition, in jar with cover, add the 3900ml dehydrated alcohol, slowly stir then, slowly add the aqueous premix that makes in the step (6);
(8) continuation slowly adds 99.6% glycerol 275ml, stirs until obtaining limpid solution;
(9) with 8 μ m, and can tolerate alcoholic acid filer element type filter it is filtered, and be sent to filling machine; In filling machine fill to 100ml slush moulding vial; Behind the sterilization gland, temperature is controlled at 25 ℃, pours iso-butane through last valve; Net volume after valve is packaged is 87.4mL, and irritating has tcm product 1.14g (about 74.7mL).
Embodiment 6
(1) Radix Astragali 300g, Fructus Schisandrae Chinensis 55g, Cortex Magnoliae Officinalis 101g and Radix Glycyrrhizae 185g are added the water logging that accounts for 8 times of amounts of its quality and steeped 2 hours, atmospheric pressure reflux was extracted 2 hours, leached solution; Add the water atmospheric pressure reflux 2 hours account for 5 times of amounts of its quality in the medical material again, leach filtrating; Merge filtrating and concentrated twice, the concentrated medicament volume is 1090ml, in concentrated solution 80% (V/V) ethanol as for 3 times of volumes, staticly settles 24 hours, filters; Filtrate recycling ethanol, concentrated filtrate, concentrated medicament volume are 765ml;
(2) with add in the above-mentioned medical material residue account for 80% (V/V) ethanol mixing of 8 times of amounts of its quality after, room temperature left standstill 24 hours, filtered; Filtrate recycling ethanol, concentrated filtrate, concentrated medicament volume are 400ml;
(3) concentrated solution that step (1) and step (2) is obtained merges, sucking filtration, vacuum drying, Chinese medicinal powder 66.92g, powder for drying is subsequent use, powder diameter 4 μ m;
(4) the 30g propolis powder is broken into 60 order powder, with the ethanol extraction of 120ml 98% (V/V) 20 hours;
(5) collect propolis extract,, concentrate and reclaim the liquid drying under reduced pressure, pulverize after being cooled to room temperature with the quiet filter made of diatomite filter, must powder 5.05g, powder for drying is subsequent use; Particle diameter 6 μ m;
(6) Chinese medicinal powder that makes in step (3) and the step (5) is joined in the 100ml purified water, stir, get the Chinese medicine aqueous premix;
(7) under the explosion-proof environment condition, in jar with cover, add the 4400ml dehydrated alcohol, slowly stir then, slowly add the aqueous premix that makes in the step (6);
(8) continuation slowly adds 99.6% glycerol 500ml, stirs until obtaining limpid solution;
(9) with 5 μ m, and can tolerate alcoholic acid filer element type filter it is filtered, and be sent to filling machine; In filling machine fill to 100ml slush moulding vial; Behind the sterilization gland, temperature is controlled at 25 ℃, pours iso-butane through last valve; Net volume after valve is packaged is 84.3mL, and irritating has tcm product 1.08g (about 75.2mL).
Modern medicine shows that Radix Astragali sweet in the mouth is warm in nature, is important QI invigorating Chinese medicine, and document records effects such as " QI invigorating benefit table, detoxification granulation promotings ", wherein contains plurality of active ingredients such as polysaccharide, osajin and Saponin class.
Radix Astragali ability anticoagulant reduces blood viscosity and coagulability, strengthens plasmin activity, relaxing smooth muscle; Expansion lung, the heart, cerebrovascular reduce vascular resistance, improve blood circulation; Especially microcirculation improvement, thus cardio-pulmonary function improved, promote pulmonary ventilation, organization aeration; The Radix Astragali can effectively reduce lipid peroxidation, reduces the generation of malonaldehyde, and the effect of stronger removing free radical is arranged; In addition, the effective ingredient astragaloside of the Radix Astragali has effects such as antiinflammatory, calmness, analgesia.
The Radix Astragali has the effect that improves body endolymph cellular immune function, and astragalus decoction has the killing activity that improves NK cells in mice, can strengthen the effects of killing tumor cell indirectly such as cytotoxic effect that reduce the inductive LAK cell of dosage L-2; After external use mouse lung macrophage added Radix Astragali extractive solution, its ability of engulfing Staphylococcus albus significantly strengthened.
Inhibitory action and enhancing antibody that the Radix Astragali can strengthen antibacterial and virus generate: the Radix Astragali can not only promote the leukocyte of mice and human peripheral that new castle disease virus or Sendai virus are induced α-IFN, promote cycloheximide, radiating streptozotocin D on people's lung diploid cell, to cause β-IFN superinduction reaction; And the proof Radix Astragali self such as Hou Shi can induce γ-IFN in mouse boosting cell is cultivated.
Propolis is distinguished the flavor of little sweet, and property is flat, and the history in existing more than 20 year of immunological adjuvant as the veterinary goes up vaccine has very strong antimicrobial and anti-inflammation efficacy.The propolis alcohol extract can reduce the permeability of areas of inflammation blood capillary, suppresses inflammatory swelling, injury of lung is had the significant protection effect, and can resist the generation of pulmonary edema, and chronic inflammatory disease is had significant consistent acting on; Can also reduce the content of peroxide metabolite MDA in the inflammation tissue in addition, the reaction occurrence degree reduces inflammation; Discover; Propolis can obviously suppress the expression of CD54 in the acute lung injury bronchus of rat epithelial cell; Stop the activation of nuclear factor NkBp65, suppress the expression of bronchus of rat epithelial cell NkBp65mRNA, thereby harmful production of cytokines is reduced; The protection body is avoided the further damage of destructive stimulus, thereby reaches effective antiinflammatory purpose.
Fructus Schisandrae Chinensis is warm in nature, and is sweet.Having astringes the lung promotes the production of body fluid, restrains astringent or styptic treatment for spontaneous sweating function, and the ethanol immersion has stronger antibacterial action external to bacillus pyocyaneus, and antivirus action is arranged in vivo.
Radix Glycyrrhizae is warm in nature, and sweet in the mouth is returned 12 warps.Invigorating the spleen and replenishing QI is arranged, lung moistening, the effect of emergency detoxifcation is in harmonious proportion hundred medicines.
Cortex Magnoliae Officinalis is warm in nature, and bitter in the mouth is sweet, returns taste lung large intestine channel.Have the dampness expectorant, the therapeutic method to keep the adverse QI flowing downwards is removed full, the effect that antiinflammatory is relievingd asthma.Main effective ingredient is a magnolol, has stronger antiviral, antibiotic, easing pain and diminishing inflammation function.
Four flavor Chinese medicine reasonable formula are not only collaborative but also complementary, share and can accurately treat respiratory disease of birds fast.
In sum, in the present invention prescription with Radix Astragali invigorating the lung and benefiting vital QI, consolidating superficial resistance; Astringe the lung with the Fructus Schisandrae Chinensis the kidney invigorating; The propolis astringing lung-QI and relieving cough, anti-inflammation; The Radix Glycyrrhizae invigorating the spleen and replenishing QI, lung moistening, emergency detoxifcation; The expectorant of Cortex Magnoliae Officinalis dampness, the therapeutic method to keep the adverse QI flowing downwards is removed full, and antiinflammatory is relievingd asthma.Full side's Synergistic has relieving cough and asthma, anti-inflammation, and functions such as detoxifcation, instant effect is applicable to the control of fowl respiratory system disease.
Aerosol can directly arrive site of action or absorption site, has fairly obvious quick-acting effects and positioning action, especially aspect respiratory tract administration, has the irreplaceable advantage of other dosage form; Drug pack can keep clean and aseptic condition in airtight container, has reduced the contaminated chance of medicine, and remaining medicine also is difficult for causing environmental pollution after the drug withdrawal.In addition, because container is opaque, lucifuge does not directly contact with moisture with airborne oxygen, so help improving stability of drug; Aerosol local application can reduce medicine gastrointestinal is stimulated, and avoids the first pass effect of liver; Medicinal aerosol is equipped with quantitative valve, and dosage is accurate.
The present invention's animal experiment of being correlated with:
Test drug: embodiment 1 said medicinal herbs aerosol inhalation agent
Clinical trial:
Get the medicinal herbs aerosol inhalation agent, test is when participating in the cintest done by plant in Tianjin, is used to treat infectious bronchitis of chicken, and main clinic symptoms shows as: spirit is depressed, and moist rale is arranged during breathing, cough etc.; Spray 1 every every day, and about 20mL used 5 days continuously; Effective percentage reaches 90-92% after the course of treatment, and cure rate reaches 76.8%.
The effect comparison test:
Get the medicinal herbs aerosol inhalation agent, the effect comparison test is done by plant in Tianjin, is used to treat infectious bronchitis of chicken, and main clinic symptoms shows as: spirit is depressed, and moist rale is arranged during breathing, cough etc.Get the sick chicken of equivalent and make an experiment, with this medicinal herbs aerosol inhalation agent and the treatment of levofloxacin injection, the aerosol group is sprayed 1 time every every day respectively in test, and about 20mL used 5 days continuously; The injection group is injected (300mg/d) 1 time every day, injects continuously 5 days.Use aerosol group effective percentage to reach 91.2% after the course of treatment, cure rate reaches 79%, and injection experimental group effective percentage is 70%, and cure rate is 63.2%.
Through above test result analysis; The medicinal herbs aerosol inhalation agent of this Chinese prescription is having significant curative effect aspect prevention and the treatment respiratory disease of birds; And aerosol is in the therapeutic process of respiratory disease of birds; Can directly arrive site of action or absorption site, have fairly obvious quick-acting effects and positioning action, especially aspect respiratory tract administration, have the irreplaceable advantage of other dosage form; Drug pack can keep clean and aseptic condition in airtight container, has reduced the contaminated chance of medicine, and remaining medicine also is difficult for causing environmental pollution after the drug withdrawal.In addition, because container is opaque, lucifuge does not directly contact with moisture with airborne oxygen, so help improving stability of drug; Aerosol local application can reduce medicine gastrointestinal is stimulated, and avoids the first pass effect of liver; Medicinal aerosol is equipped with quantitative valve, and dosage is accurate.
Above-mentioned detailed description of this a kind of method for preparing that is used to treat the medicinal herbs aerosol inhalation agent of fowl respiratory system disease being carried out with reference to embodiment; Be illustrative rather than determinate; Can enumerate out several embodiment according to institute's limited range; Therefore in the variation and the modification that do not break away under the general plotting of the present invention, should belong within protection scope of the present invention.
Claims (10)
1. method for preparing that is used to treat the medicinal herbs aerosol inhalation agent of fowl respiratory system disease, it is characterized in that: concrete steps are:
(1) Radix Astragali, Fructus Schisandrae Chinensis, Cortex Magnoliae Officinalis and the Radix Glycyrrhizae back atmospheric pressure reflux that is soaked in water is extracted, leached solution and concentrate, concentrated solution places 80%V/V ethanol to staticly settle, and getting filtrating concentrating, and medical material weight and concentrated solution volume are than counting 1 by grams per milliliter: 1-1.2;
(2) with after adding 80%V/V ethanol mixing in the above-mentioned medical material residue, room temperature leaves standstill filtration, and concentrated filtrate, medical material weight and concentrated solution volume are than counting 1 by grams per milliliter: 0.6-1.2;
(3) concentrated solution that step (1) and step (2) is obtained merges, sucking filtration, and vacuum drying, powder for drying is subsequent use;
(4) propolis was pulverized the back with dehydrated alcohol extraction 12-24 hour, medical material weight and dehydrated alcohol volume ratio count 1 by grams per milliliter: 3-6;
(5) collect propolis extract, with the quiet filter made of diatomite filter, concentrate recovery liquid drying under reduced pressure, pulverize after being cooled to room temperature, powder for drying is subsequent use;
(6) Chinese medicinal powder that makes in step (3) and the step (5) is joined in deionized water or the purified water, this Chinese medicinal powder weight and deionized water or purified water volume ratio count 1 by grams per milliliter: 1-2, stir, and get the Chinese medicine aqueous premix;
(7) under the explosion-proof environment condition, in jar with cover, add dehydrated alcohol, slowly stir then, slowly add the Chinese medicine aqueous premix that makes in the step (6), by its volume parts dehydrated alcohol 85.9-92.2 part, Chinese medicine aqueous premix 1.65-4.4 part;
(8) continue slowly to add glycerol 6.1-11.9 part, stir until obtaining limpid solution by its volume parts 99.6%;
(9) filter filters, and the filling machine fill is poured propellant behind the sterilization gland;
Wherein, the above-mentioned Radix Astragali, propolis, Fructus Schisandrae Chinensis, Cortex Magnoliae Officinalis and Radix Glycyrrhizae are got Radix Astragali 44.4-64.5 part, propolis 3.45-6.45 part, Fructus Schisandrae Chinensis 6.67-12.5 part, Cortex Magnoliae Officinalis 19.4-20.8 part, Radix Glycyrrhizae 34.5-44.5 part by its parts by weight.
2. the method for preparing that is used to treat the medicinal herbs aerosol inhalation agent of fowl respiratory system disease according to claim 1, it is characterized in that: concrete steps are:
(1) Radix Astragali, Fructus Schisandrae Chinensis, Cortex Magnoliae Officinalis and Radix Glycyrrhizae are added the water logging that accounts for 8 times of amounts of its quality and steeped 2 hours, atmospheric pressure reflux was extracted 2 hours, leached solution; Add the water atmospheric pressure reflux 2 hours account for 5 times of amounts of its quality in the medical material again, leach filtrating; Merge twice filtrating and concentrate, medical material weight and concentrated solution volume are than counting 1 by grams per milliliter: 1-2, concentrated solution is placed the 80%V/V ethanol of 3 times of volumes, and staticly settled filtration 24 hours; Filtrate recycling ethanol, concentrated filtrate, medical material weight and concentrated solution volume are than counting 1 by grams per milliliter: 1-1.2;
(2) with add in the above-mentioned medical material residue account for the 80%V/V ethanol mixing of 8 times of amounts of its quality after, room temperature left standstill 24 hours, filtered; Filtrate recycling ethanol, concentrated filtrate, medical material weight and concentrated solution volume are than counting 1 by grams per milliliter: 0.6-1.2;
(3) concentrated solution that step (1) and step (2) is obtained merges, sucking filtration, and vacuum drying, powder for drying is subsequent use, the powder diameter range: 0.5-10 μ m;
(4) propolis powder is broken into 60-80 order powder, with dehydrated alcohol extraction 12-24 hour, medical material weight and dehydrated alcohol volume ratio counted 1 by grams per milliliter: 3-6;
(5) collect propolis extract, with the quiet filter made of diatomite filter, concentrate recovery liquid drying under reduced pressure, pulverize after being cooled to room temperature, powder for drying is subsequent use; Particle size range: 0.5-10 μ m;
(6) Chinese medicinal powder that makes in step (3) and the step (5) is joined in deionized water or the purified water, this Chinese medicinal powder weight and deionized water or purified water volume ratio count 1 by grams per milliliter: 1-2, stir, and get the Chinese medicine aqueous premix;
(7) under the explosion-proof environment condition, in jar with cover, add dehydrated alcohol, slowly stir then, slowly add the Chinese medicine aqueous premix that makes in the step (6), by its volume parts dehydrated alcohol 85.9-92.2 part, Chinese medicine aqueous premix 1.65-4.4 part;
(8) continue slowly to add glycerol 6.1-11.9 part, stir until obtaining limpid solution by its volume parts 99.6%;
(9) with 2-10 μ m, and can tolerate alcoholic acid filer element type filter filtration, the filling machine fill is poured propellant behind the sterilization gland;
Wherein, the above-mentioned Radix Astragali, propolis, Fructus Schisandrae Chinensis, Cortex Magnoliae Officinalis and Radix Glycyrrhizae are got Radix Astragali 44.4-64.5 part, propolis 3.45-6.45 part, Fructus Schisandrae Chinensis 6.67-12.5 part, Cortex Magnoliae Officinalis 19.4-20.8 part, Radix Glycyrrhizae 34.5-44.5 part by its parts by weight.
3. the method for preparing that is used to treat the medicinal herbs aerosol inhalation agent of fowl respiratory system disease according to claim 1 and 2, it is characterized in that: said propellant is one or more in tetrafluoroethane, heptafluoro-propane or the iso-butane.
4. the method for preparing that is used to treat the medicinal herbs aerosol inhalation agent of fowl respiratory system disease according to claim 1 and 2 is characterized in that: dehydrated alcohol, 99.6% glycerol and deionized water or purified water umber meter by volume in said step (6)-(8): dehydrated alcohol 85.9-92.2 part, glycerol 6.1-11.9 part, deionized water or purified water 1.6-2.2 part.
5. the method for preparing that is used to treat the medicinal herbs aerosol inhalation agent of fowl respiratory system disease according to claim 1 and 2 is characterized in that: get 50 parts of the Radixs Astragali, 5 parts of propolis, 12 parts of Fructus Schisandrae Chinensis, 20 parts of Cortex Magnoliae Officinalis, 40 parts in Radix Glycyrrhizae by its parts by weight.
6. the method for preparing that is used to treat the medicinal herbs aerosol inhalation agent of fowl respiratory system disease according to claim 1 and 2 is characterized in that: get 64.4 parts of the Radixs Astragali, 6.4 parts of propolis, 6.68 parts of Fructus Schisandrae Chinensis, 19.4 parts of Cortex Magnoliae Officinalis, 34.6 parts in Radix Glycyrrhizae by its parts by weight.
7. the method for preparing that is used to treat the medicinal herbs aerosol inhalation agent of fowl respiratory system disease according to claim 1 and 2 is characterized in that: get 44.4 parts of the Radixs Astragali, 3.5 parts of propolis, 12.4 parts of Fructus Schisandrae Chinensis, 20.8 parts of Cortex Magnoliae Officinalis, 44.4 parts in Radix Glycyrrhizae by its parts by weight.
8. the method for preparing that is used to treat the medicinal herbs aerosol inhalation agent of fowl respiratory system disease according to claim 1 and 2 is characterized in that: get 55 parts of the Radixs Astragali, 5.6 parts of propolis, 9.6 parts of Fructus Schisandrae Chinensis, 20.4 parts of Cortex Magnoliae Officinalis, 38 parts in Radix Glycyrrhizae by its parts by weight.
9. the method for preparing that is used to treat the medicinal herbs aerosol inhalation agent of fowl respiratory system disease according to claim 1 and 2 is characterized in that: get 48 parts of the Radixs Astragali, 4 parts of propolis, 8 parts of Fructus Schisandrae Chinensis, 19.6 parts of Cortex Magnoliae Officinalis, 42 parts in Radix Glycyrrhizae by its parts by weight.
10. the method for preparing that is used to treat the medicinal herbs aerosol inhalation agent of fowl respiratory system disease according to claim 1 and 2 is characterized in that: get 60 parts of the Radixs Astragali, 6 parts of propolis, 11 parts of Fructus Schisandrae Chinensis, 20.2 parts of Cortex Magnoliae Officinalis, 37 parts in Radix Glycyrrhizae by its parts by weight.
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