CN106236895A - A kind of Chinese medicine treating cough and preparation method thereof - Google Patents
A kind of Chinese medicine treating cough and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a kind of Chinese medicine treating cough and preparation method thereof, be made up of the crude drug of following parts by weight: 25 35 parts of Folium Mori；Semen Armeniacae Amarum 25 35 parts；Fructus Arctii 15 25 parts；Radix Peucedani 5 15 parts；Herba Menthae 5 15 parts；Pericarpium Citri Reticulatae 5 15 parts；Radix Platycodonis 5 15 parts；38 parts of Radix Glycyrrhizae；Pericarpium Citri Reticulatae and the extracted volatile oil of Herba Menthae, obtain clathrate through HP beta cyclodextrin inclusion, and Fructus Arctii, Radix Peucedani use alcohol reflux, Folium Mori, Semen Armeniacae Amarum, Radix Platycodonis, Radix Glycyrrhizae and medicinal residues are through soak by water, it is prepared as preparation, there is the effect for the treatment of cough, especially for the cough of wind-heat invading the lung；Using natural plants, the most do not find obvious toxic and side effects, safety is high, and this product is made the oral solutions can made pharmaceutically, can be widely used.
Description
Technical field
The present invention relates to technical field of Chinese medicines, particularly relate to a kind of Chinese medicine treating cough and preparation method and application.
Background technology
Cough refers to impairment of dispersing and descending function of the lung, abnormal rising of lung-QI, sends and coughs sound, or coughs a kind of diseases of pulmonary system of liquid of spitting.The traditional Chinese medical science is thought,
Cough is the impairment of dispersing and descending function of the lung caused by exopathogen infringement lungs or visceral dysfunction, and caused by abnormal rising of lung-QI, and Ming Dynasty is opened
Jing Yue, in Jing-Yue Complete Works, is summarized as diseases caused by exogenous pathogenic factor, the big class of internal injury two cough first.From modern medical theory, cough is
Defense mechanism in the one of body, is conducive to removing respiratory secretions and harmful substance, stops the diffusion of respiratory tract infection, modern
Medical research confirm in the syndromic types of cough most commonly seen with exogenous cough again.
Along with social city, technicalization, the development of hommization, health care industry has obtained vigorous growth, more comes
The most consumers focus on the health of self, and the main pathological characteristic that cough is formed is impairment of dispersing and descending function of the lung.Anemopyretic cough is by machine
Body-sensing is by the heresy of wind heat, the cough caused by impairment of purifying function of the lung.Clinical visible cough, dry cough without phlegm or expectorant Huang are thick, or heating, and sweating is disliked
Wind, xerostomia pharyngalgia, nose stream yellow thick nasal discharge, red tongue with thin and yellow fur, floating and rapid pulse etc., more common with the autumn in summer.Control suitable wind and heat dispersing, lung qi dispersing cough-relieving.
The most for a long time, the drug safety exclusive due to Chinese medicine and therapeutic effect are good and welcome by patient for treatment by Chinese herbs history, with
And come is the increase of Chinese medicine usage amount, and universal along with humanity concept, and the composition of novel harmonious society, study one
Plant Chinese medicine treating cough and preparation method thereof to be very important.
The medicine for the treatment of cough at present, mainly uses Western medicine, such as: codeine, Ethanedisulfonate, carbetapentane citrate etc., prolonged application can
The side effect such as addiction, dizziness and nausea, constipation can be caused, and the medicine with Chinese crude drug as raw material can well be avoided, and these poison are secondary to be made
With.
Summary of the invention
The present invention provides a kind of evident in efficacy, Chinese medicine for the treatment of cough that side effect is little and preparation method thereof, solves existing
The problems such as Western medicine side effect is big.
The present invention is by the following technical solutions: a kind of Chinese medicine treating cough, is made up of the crude drug of following parts by weight:
Folium Mori 25-35 part, Semen Armeniacae Amarum 25-35 part, Fructus Arctii 15-25 part, Radix Peucedani 5-15 part, Herba Menthae 5-15 part, Pericarpium Citri Reticulatae 5-15 part, Radix Platycodonis
5-15 part, Radix Glycyrrhizae 3-8 part.
As a preferred technical solution of the present invention: its composition with parts by weight representation is: 30 parts of Folium Mori, hardship
30 parts of Semen Armeniacae Amarum, Fructus Arctii 20 parts, Radix Peucedani 10 parts, Herba Menthae 10 parts, Pericarpium Citri Reticulatae 10 parts, Radix Platycodonis 10 parts, 5 parts of Radix Glycyrrhizae.
As a preferred technical solution of the present invention: described Semen Armeniacae Amarum is Semen Armeniacae Amarum, Fructus Arctii is Fructus Arctii (parched).Cough
Type of coughing is anemopyretic cough.
A kind of method of the Chinese medicine preparing described treatment cough, step is:
A: weigh Herba Menthae and Pericarpium Citri Reticulatae by the ratio of weight and number of described component, is broken into coarse powder, uses steam distillation to carry
Obtaining volatile oil, the volatile oil obtained becomes clathrate through HP-beta-cyclodextrin inclusion compound again, and the another extracting container of aqueous solution after distillation is received
Collection；
B: weigh Radix Peucedani and Fructus Arctii by the mass fraction ratio of described component, through alcohol reflux, filters, reclaims second
Alcohol, is concentrated to give alcohol extraction concentrated solution；
C: weigh by the mass fraction ratio of described component and extract volatilization in Folium Mori, Semen Armeniacae Amarum, Radix Platycodonis and Radix Glycyrrhizae, with step a
Medicinal residues and step b medicinal residues after the alcohol reflux that volumetric concentration is 70% after oil merge, and through soak by water, add step
The aqueous solution after vapor distillation in a, filters, and concentrates, and cooling adds ethanol, and precipitate with ethanol obtains water extract-alcohol precipitation concentrated solution, by water extracting alcohol
Heavy concentrated solution merges with the alcohol extraction concentrated solution of step b, makes oral liquid first product after concentration；
D: mixed with the clathrate in step a by the oral liquid first product in step c, regulation pH value, adds adjuvant and is prepared as mouth
Take liquid finished product, high temperature sterilize, fill.
As a preferred technical solution of the present invention: in step a, vapor distillation extracts volatile oil, and during extraction, decoction pieces takes
Coarse powder, the amount added water is 10 times amount of medical material weight, soaks 0.5 hour, vapor distillation 7 hours；Add 8 times of weight of volatile oil
HP-β-CD, uses polishing to prepare HP-β-CD volatile oil clathrate compound, grinds inclusion 95min, and clathrate is in 40 DEG C of vacuum drying
The most standby.
As a preferred technical solution of the present invention: in step b, added amount of alcohol is 15 times amount of crude drug weight, second
Alcohol volumetric concentration is 70%, point 3 reflux, extract, every time backflow 1.5 hours, and united extraction liquid filters, and filtrate reduced in volume returns
Receive ethanol, obtain alcohol extraction concentrated solution.
As a preferred technical solution of the present invention: the amount added water in step c is 20 times of crude drug weight, decoct 2
Secondary, each 2 hours, merge twice decocting liquid, filter, surveying relative density when concentrating filtrate to 60 DEG C is 1g/ml, cold
But, adding ethanol and reach 60% to alcohol content, stir, centrifugal after placing 48 hours, centrifuge speed 4800 revs/min, it is heavy to discard
Forming sediment, reclaim centrifugal liquid, merge with alcohol extraction concentrated solution in step b, concentrating under reduced pressure obtains oral liquid first product.
A kind of Chinese medicine treating cough of the present invention and preparation method thereof uses above technical scheme and prior art phase
Ratio, have following technical effect that 1, the Chinese medicine preparation of the present invention shows have preferable antitussive through the pharmacological results, eliminate the phlegm and
Antiinflammatory action；2, using natural plants, the most do not find obvious toxic and side effects, safety is high, and this product is made can
To make tablet, capsule or oral liquid pharmaceutically.3, the theory of Chinese medical science basis of the present invention: curing mainly: affection due to external wind and heat, lung defends mistake
A surname, treatment anemopyretic cough.The rule for the treatment of: solution of dispeling the wind (dissipating) heat, lung qi dispersing slits (change) expectorant, cough-relieving sore-throat relieving.Fang Xie: Folium Mori, Semen Armeniacae Amarum---
Wind-dispelling heat-dissipating, expectorant cough suppressant and anti-asthmatic, for monarch drug；Fructus Arctii, Radix Peucedani, Herba Menthae---removing heat from the lung and dissipating phlegm is relievingd asthma, Pericarpium Citri Reticulatae, Radix Platycodonis---open a surname
Lung qi, eliminate the phlegm promoting the circulation of QI, for ministerial drug；Radix Glycyrrhizae---nourishing the lung to arrest cough, for adjuvant, coordinating the actions of various ingredients in a prescription.
Detailed description of the invention
Below in conjunction with example, the invention will be further described, and embodiment is only used for that the present invention will be described, not
Constituting the restriction to right, other alternative means that it may occur to persons skilled in the art that, all in right of the present invention
In claimed range.
In the present invention, Folium Mori are the dried leaves of moraceae plants Mulberry Morus alba L..Semen Armeniacae Amarum is rosaceous plant Prunus armeniaca L.var.ansu Maxim.
Prunus armeniaca L.var.ansu Maxim., Siberia Fructus Pruni Prunus sibirica L., prunus mandshuricaKoehne Prunus
Mandshurica (Maxim.) Koehne or the dry mature seed of Fructus Pruni Prunus armeniaca L..Radix Peucedani is that Umbelliferae is planted
The dry root of thing Peucedanum praeruptorum DUNN Peucedanum praeruptorum Dunn.Fructus Arctii is feverfew Fructus Arctii Arctium
The dry mature fruit of lappa L..Herba Menthae is going up dryly of labiate Herba Menthae Mentha haplocalyx Briq.
Part.Pericarpium Citri Reticulatae is rutaceae orange Citrus reticulata Blanco and the dry mature skin of variety thereof.Fructus Citri tangerinae
Stalk is the dry root of Campanulaceae Radix Platycodonis Platycodon grandiflorum (Jacq.) A.DC..Radix Glycyrrhizae is leguminous plant
Radix Glycyrrhizae Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Bat. Glycyrrhiza inflata Bat. or Glycyrrhiza glabra L.
The dry root of Glycyrrhiza glabra L. and rhizome.Concentration of alcohol described herein is volumetric concentration.
Embodiment 1:
The first step: weigh Folium Mori 180g, Semen Armeniacae Amarum 180g, Fructus Arctii 120g, Radix Peucedani 90g, Herba Menthae 60g, Radix Platycodonis 60g,
Pericarpium Citri Reticulatae 60g, Radix Glycyrrhizae 30g.
Second step: take Herba Menthae and Pericarpium Citri Reticulatae, be broken into coarse powder, adds 1200g water, soaks 0.5 hour, by vapor distillation 7
Hour, collect volatile oil, volatile oil adds 8 times amount HP-β-CD, uses polishing to prepare clathrate, grinds inclusion 95min, inclusion
Thing 40 DEG C is standby after being vacuum dried.
3rd step: take Fructus Arctii and Radix Peucedani, adds the ethanol of 70% volumetric concentration of two kinds of crude drug gross weight 15 times amount, point
Three reflux, extract, each 1.5 hours, united extraction liquid, filter, filtrate reduced in volume reclaims ethanol, is concentrated into without alcohol taste,
Alcohol extraction concentrated solution.
4th step: take the medicinal residues after extracting Herba Menthae after volatile oil, the medicinal residues of Pericarpium Citri Reticulatae and Fructus Arctii, Pericarpium Citri Reticulatae alcohol extraction and Folium Mori,
Semen Armeniacae Amarum, Radix Platycodonis and Radix Glycyrrhizae merge, and add the water of gross weight 20 times amount, decoct 2 times, each 2 hours, merge secondary decocting liquid with
Herba Menthae, Pericarpium Citri Reticulatae extract the aqueous solution after volatile oil, filter, and the relative density measured when concentrating the filtrate to 60 DEG C is 1g/ml, cold
But, add ethanol and reach 60% to alcohol content, stir evenly, centrifugal after placing 48 hours, centrifugal speed 4800 revs/min, discard precipitation, reclaim
Ethanol, merges with the alcohol extraction concentrated solution of Fructus Arctii and Radix Peucedani, is concentrated into relative density 1.12～1.15 (60 DEG C), add water adjust to
950ml, regulation pH value is 6.0～7.0, adds preservative, correctives, stands 24 hours, centrifugal, takes supernatant, and add water adjustment
To 1000ml, sterilizing, fill, both obtained.
Embodiment 2: pharmacodynamic experiment
(1) cough-relieving experiment
1. method
Laboratory sample is prepared by the embodiment of the present invention 1.Take ICR mice 48, body weight 20～22g, male and female half and half.By body weight
It is randomly divided into 4 groups: respectively (1) blank group: NS 10ml/kg；(2) Ganmao Kesou Granules group: 5g/kg；(3) present invention
Low dose group: 2.8g/kg；(4) high dose group of the present invention: 5.6g/kg.Each group is pressed the volume of 20ml/kg every day by above-mentioned dosage
Gastric infusion, every day 1 time, continuous 6 days.White mice is placed in inverted 500ml beaker after being administered 30min by last, inside puts one cotton
Ball, draws ammonia 0.2ml with 1ml syringe, injects cotton balls, is inverted rapidly beaker, observes and record the cough latent period of mice
With 3 minutes in cough number of times.
2. result
Experimental result shows: Ganmao Kesou Granules group and two dosage groups of the present invention can extend mouse cough incubation period,
Compare with blank group and there is pole significant difference (p ＜ 0.01), and mice can be reduced cough in 3 minutes number of times, wherein feel
Emit cough granule group, high dose group of the present invention with blank group comparing difference significantly (p ＜ 0.01).Show that the present invention has town
Cough effect.It is shown in Table 1.
Table 1 present invention impact on ammonia induced mice Respiratory frequency
* p < 0.01 compares with blank group
(2) reduce phlegm experiment
1. method
Laboratory sample is prepared by the embodiment of the present invention 1.Take ICR mice 30, body weight 20～22g, be randomly divided into 4 by body weight
Group: respectively (1) blank group: NS 10ml/kg；(2) Ganmao Kesou Granules group: 5g/kg；(3) low dose group of the present invention:
2.8g/kg；(4) high dose group of the present invention: 5.6g/kg.Each group is pressed the volume gastric infusion of 20ml/kg every day by above-mentioned dosage,
Every day 1 time, continuous 6 days.White mice fasting 8～12h before experiment.After last is administered 1h, each mouse peritoneal injection phenol red solution
0.5m1.Again after 30min, de-cervical vertebra is put to death, and faces upward position and fixes.Cervical region stretches, anatomical isolation escape pipe.One is worn under trachea
Line is in case fixing tracheal intubation is used.Tracheal intubation (No. 9 syringe needles polished with head replace) inserts tracheal strips at thyroid cartilage
About 0.3cm is fixing with the silk thread ligation got ready.5%NaHCO is drawn with 1ml syringe3Solution 0.5ml, injects into tracheal strips.
Push away the most continuously and take out 3 times, lavation respiratory tract.Finally with syringe, irrigating solution is extracted out in injecting tube.Repeat 2 as stated above
Secondary, merge the washing liquid about 1.2m1,2000rpm of 3 times and take supernatant microplate reader after being centrifuged 10min at wavelength 546nm, survey OD
Value.
2. result
Experimental result shows: two dosage groups of the present invention can dramatically increase mice trachea PSP, with blank group ratio
Relatively significant difference (p < 0.01)；Ganmao Kesou Granules group also can increase mice trachea PSP, but compares with blank group
Difference is not notable (p ＞ 0.05).Show that phlegm-dispelling functions of the present invention is obvious.It is shown in Table 2.
Table 2 present invention impact on white mice trachea PSP
* p < 0.01 compares with blank group
(3) antiinflammatory experiment
1. method
Laboratory sample is prepared by the embodiment of the present invention 1.Take ICR mice 34, body weight 20～22g, be randomly divided into 3 by body weight
Group: respectively (1) blank group: NS 10ml/kg；(2) low dose group of the present invention: 2.8g/kg；(3) high dose of the present invention
Group: 5.6g/kg.Each volume gastric infusion pressing 20ml/kg by above-mentioned dosage every day of organizing, every day 1 time, continuous 6 days.Last is administered
It is applied to mice left ear both sides with dimethylbenzene 0.03ml after 1h.After causing scorching 1h, put to death mice, cut left and right two ears, use card punch
(diameter 9mm) takes off round auricle, scales/electronic balance weighing respectively at same position, calculates auricular concha weight.Respectively group mice auricular concha
Swelling (left auricular concha weight-auris dextra shell weight) and inhibitory rate of intumesce.Computing formula is as follows:
2. result
Experimental result shows: two dosage groups of the present invention all can significantly improve mice caused by dimethylbenzene xylene ear swelling, right with blank
Compare according to group and there is pole significant difference (p < 0.01).Show that the present invention has antiinflammatory action.It is shown in Table 3.
Table 3 xylol of the present invention causes the impact of mice ear
* p < 0.01 compares with blank group.
Claims (9)
1. the Chinese medicine treating cough, it is characterised in that: it is made up of the crude drug of following parts by weight: Folium Mori 25-35 part, hardship
Semen Armeniacae Amarum 25-35 part, Fructus Arctii 15-25 part, Radix Peucedani 5-15 part, Herba Menthae 5-15 part, Pericarpium Citri Reticulatae 5-15 part, Radix Platycodonis 5-15 part, Radix Glycyrrhizae 3-8
Part.
The Chinese medicine for the treatment of cough the most according to claim 1, it is characterised in that: by the crude drug system of following parts by weight
Become: 30 parts of Folium Mori, Semen Armeniacae Amarum 30 parts, Fructus Arctii 20 parts, Radix Peucedani 10 parts, Herba Menthae 10 parts, Pericarpium Citri Reticulatae 10 parts, Radix Platycodonis 10 parts, Radix Glycyrrhizae 5
Part.
The Chinese medicine for the treatment of cough the most according to claim 1, it is characterised in that: described Semen Armeniacae Amarum is Semen Armeniacae Amarum, Fructus Arctii
Son is Fructus Arctii (parched).
4. the Chinese medicine for the treatment of cough as claimed in claim 1, it is characterised in that preparation method comprises the following steps:
A: weigh Herba Menthae and Pericarpium Citri Reticulatae by the ratio of weight and number of described component, is broken into coarse powder, uses extraction by steam distillation to obtain
Volatile oil, the volatile oil obtained becomes clathrate through HP-beta-cyclodextrin inclusion compound again, and the another extracting container of aqueous solution after distillation is collected；
B: weigh Radix Peucedani and Fructus Arctii by the mass fraction ratio of described component, through alcohol reflux, filters, and reclaims ethanol, dense
Contract to obtain alcohol extraction concentrated solution；
C: weigh by the mass fraction ratio of described component after extracting volatile oil in Folium Mori, Semen Armeniacae Amarum, Radix Platycodonis and Radix Glycyrrhizae, with step a
Medicinal residues and step b medicinal residues after the alcohol reflux that volumetric concentration is 70% merge, through soak by water, add in step a
Vapor distillation after aqueous solution, filter, concentrate, cooling, add ethanol, precipitate with ethanol obtains water extract-alcohol precipitation concentrated solution, by water extract-alcohol precipitation
Concentrated solution merges with the alcohol extraction concentrated solution of step b, makes oral liquid first product after concentration；
D: mixed with the clathrate in step a by the oral liquid first product in step c, regulation pH value, adds adjuvant and is prepared as oral liquid
Finished product, high temperature sterilize, fill.
5. the Chinese medicine for the treatment of cough as claimed in claim 4, it is characterised in that: preparation method step a is extracted volatile oil, carries
When taking, decoction pieces takes coarse powder, and the amount added water is 10 times amount of medical material weight, soaks 0.5 hour, vapor distillation 7 hours；Add volatilization
HP-β-the CD of 8 times of weight of oil, uses polishing to prepare HP-β-CD volatile oil clathrate compound, grinds inclusion 95min, and clathrate is in 40
DEG C vacuum drying after standby.
6. the Chinese medicine for the treatment of cough as claimed in claim 4, it is characterised in that: in preparation method step b, added amount of alcohol is
15 times amount of crude drug weight, ethanol volumetric concentration is 70%, point 3 reflux, extract, every time backflow 1.5 hours, united extraction
Liquid, filters, and filtrate reduced in volume reclaims ethanol, obtains alcohol extraction concentrated solution.
7. the Chinese medicine for the treatment of cough as claimed in claim 4, it is characterised in that: the amount added water in preparation method step c is former
Material 20 times of medicine weight, decocts 2 times, each 2 hours, and merge after twice decocting liquid and Herba Menthae, Pericarpium Citri Reticulatae extraction volatile oil is water-soluble
Liquid, filters, and surveying relative density when concentrating filtrate to 60 DEG C is 1g/ml, and cooling adds ethanol and reaches 60% to alcohol content, stirs
Mix uniformly, centrifugal after placing 48 hours, centrifuge speed 4800 revs/min, discard precipitation, reclaim alcohol extraction in centrifugal liquid, with step b
Concentrated solution merges, and concentrating under reduced pressure obtains oral liquid first product.
8. the application treated in the medicine coughed prepared by the Chinese medicine treating cough described in claim 1 or 2.
9. the Chinese medicine for the treatment of cough as claimed in claim 8 application in the medicine of preparation treatment cough, it is characterised in that: institute
State cough to cough for anemopyretic.
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| CN201610934778.9A CN106236895A (en) | 2016-10-25 | 2016-10-25 | A kind of Chinese medicine treating cough and preparation method thereof |
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| CN201610934778.9A CN106236895A (en) | 2016-10-25 | 2016-10-25 | A kind of Chinese medicine treating cough and preparation method thereof |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610934778.9A Pending CN106236895A (en) | 2016-10-25 | 2016-10-25 | A kind of Chinese medicine treating cough and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112587602A (en) * | 2020-12-23 | 2021-04-02 | 上海市第七人民医院(上海中医药大学附属第七人民医院) | Traditional Chinese medicine composition for relieving cough and reducing sputum, extract and preparation method and application thereof |
| CN116808163A (en) * | 2023-06-16 | 2023-09-29 | 南京市中医院 | Traditional Chinese medicine composition for clearing lung and toxin, preparation method thereof and traditional Chinese medicine and western medicine composition containing same |
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Cited By (3)
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| CN112587602A (en) * | 2020-12-23 | 2021-04-02 | 上海市第七人民医院(上海中医药大学附属第七人民医院) | Traditional Chinese medicine composition for relieving cough and reducing sputum, extract and preparation method and application thereof |
| CN112587602B (en) * | 2020-12-23 | 2022-03-08 | 上海市第七人民医院(上海中医药大学附属第七人民医院) | Traditional Chinese medicine composition for relieving cough and reducing sputum, extract and preparation method and application thereof |
| CN116808163A (en) * | 2023-06-16 | 2023-09-29 | 南京市中医院 | Traditional Chinese medicine composition for clearing lung and toxin, preparation method thereof and traditional Chinese medicine and western medicine composition containing same |
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