CN101708252B - Compound Chinese medicinal preparation for treating lung cancer and preparation method thereof - Google Patents
Compound Chinese medicinal preparation for treating lung cancer and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a compound Chinese medicinal preparation for treating lung cancer, a preparation method thereof and application thereof. The compound Chinese medicinal preparation is prepared from the following raw material medicaments in percentage by weight: 20 to 40 percent of hemp-leaved vitex, 20 to 40 percent of astragalus, 10 to 20 percent of java brucea fruit, 10 to 20 percent of ark shell, 10 to 20 percent of descurainia sophia (L.) webb ex prantl and 8 to 20 percent of liquorice root. Pharmacological tests and clinical experiments show that the compound Chinese medicinal preparation provided by the invention has good effects of relieving pain and strengthening the immunity, can effectively resist the non-small-cell lung cancer, inhibit the metastasis and diffusion of cancer cells, prevent the growth of tumors and reduce the toxic and side effect of chemotherapy on human bodies, and is low in adverse reaction. The preparation method for the compound Chinese medicinal preparation provided by the invention combines the traditional technique and the modern technique, adopts the most appropriate and effective extraction and preparation technology for different raw medicament materials, and has the advantages of strong operability, low production cost, and suitability for industrial production.
Description
Technical field
The present invention relates to a kind of medicine, be specifically related to the application of a kind of Chinese traditional compound medicine and preparation method thereof and this Chinese traditional compound medicine.
Background technology
Pulmonary carcinoma (Carcinoma of the lung) is meant and the cancer that is primary in bronchus and lung has become one of human modal malignant tumor at present.Other system tumor of its pathogenesis and whole body is the same, it be not immediately clear.Certainly, smoking is one of main inducing of pulmonary carcinoma, and the occupational factor, atmospheric pollution and ionizing radiation etc. also may be the inducements of pulmonary carcinoma.In the city, the lung cancer morbidity rate is per 100,000 people 49.19 people that fall ill, and ranks cancer morbidity first place, city; In the rural area, the lung cancer morbidity rate is per 100,000 people 33.52 people that fall ill, and ranks the 4th of city cancer morbidity.
Pulmonary carcinoma is divided into small cell lung cancer and nonsmall-cell lung cancer by its biological characteristics.Small cell lung cancer should be taked Comprehensive Treatment; Nonsmall-cell lung cancer should first-selectedly be performed the operation, and according to circumstances adds other treatment after surgery again; Recessive cancer is answered strict tracing study, and regularly X line and bronchoscopy are treated after locating as early as possible.
Mostly the medicine that is widely used in lung cancer therapy in the market is chemical drugs and biological product, wherein especially with targeting preparation, antimetabolite is in the majority, but not only price is higher in these line tumor medications, and all existence untoward reaction in various degree: the bone marrow depression side effect is arranged as docetaxel, recombinant human vascular endothelial inhibin has comparatively significantly heart condition reaction, Erlotinib has the interstitial lung disease (ILD) up to 0.8% to bring out rate, and also ubiquity gastrointestinal tract, alopecia and the local irritant untoward reaction in various degree of chemical anticancer drugs.For reducing untoward reaction, need implement to follow the tracks of treatment when using therapeutic alliance, constantly check and reinforcement monitoring, these have not only increased the risk of treatment, also make troubles to treatment, add heavy patient's economy and psychological burden, it is good to be badly in need of a kind of therapeutic effect on the market, the lung cancer therapy medicine that untoward reaction is low.
The method that the Chinese medicine preparation method often uses repeatedly decocting to boil, though water soluble ingredient extracts fully, active constituent content is not high, the medicinal liquid dose is big, takes inconvenience, is difficult for preserving; And extract technology of preparing now, and though on the efficient of extracts active ingredients and accuracy, improve a lot, under the inadequate situation of research, tend to the effective ingredient that some are unknown remove, influence the whole curative effect of Chinese medicinal formulae.
Summary of the invention
Goal of the invention: the objective of the invention is to solve the deficiencies in the prior art, a kind of preparation method for the treatment of Chinese traditional compound medicine He this Chinese traditional compound medicine of pulmonary carcinoma is provided, and another object of the present invention is the application of this Chinese traditional compound medicine in preparation treatment lung-cancer medicament.
Technical scheme: in order to realize above purpose, Chinese traditional compound medicine provided by the present invention is made by the following raw materials by weight percent medicine: Herba Viticis Cannabifoliae 20%～40%, the Radix Astragali 20%～40%, Fructus Bruceae 10%～20%, Concha Arcae 10%～20%, Semen Descurainiae 10%～20%, Radix Glycyrrhizae 8%～20%.
As preferred version, Chinese traditional compound medicine provided by the invention is made by the following raw materials by weight percent medicine: Herba Viticis Cannabifoliae 25%～33%, the Radix Astragali 21%～30%, Fructus Bruceae 14%～18%, Concha Arcae 10%～17%, Semen Descurainiae 10%～16%, Radix Glycyrrhizae 10%～18%.
Chinese traditional compound medicine provided by the invention can become granule, capsule, Tabules medicine with each extracts of bulk drugs with pharmaceutically acceptable preparing carriers.
When the extract of each raw material of Chinese traditional compound medicine provided by the invention is made tablet,, add magnesium stearate lubricant when needing each raw material and lactose or corn starch, mix homogeneously, granulate, tabletting is made tablet then.
When the extract of each raw material of Chinese traditional compound medicine provided by the invention is made capsule the extract of each raw material and carrier lactose or corn starch mix homogeneously, granulate, the encapsulated then capsule of making.
When the extract of each raw material of Chinese traditional compound medicine provided by the invention is made granule, the extract of each raw material and diluent lactose or corn starch mix homogeneously, granulate, drying is made granule.
The preparation method of Chinese traditional compound medicine provided by the invention may further comprise the steps:
(1) get Herba Viticis Cannabifoliae, break into coarse powder, 50%～70% ethanol that adds 5～10 times of amounts of Herba Viticis Cannabifoliae medical material is heated to 20～50 ℃ as the desorption agent, soaks 15min～45min; 20%～50% alcohol reflux 3 times that adds 5～10 times of amounts of Herba Viticis Cannabifoliae medical material then, each 1～1.5 hour, merge extractive liquid, concentrated, D101 macroporous resin on the concentrated solution, with 70%～90% ethanol elution, decompression recycling ethanol, spray drying is standby;
(2) get the Radix Astragali, break into coarse powder, add 5% alcoholic solution of 5～10 times of amounts of medical material amount, ultrasonic extraction twice was extracted merge extractive liquid, 1.5 hours at every turn; Extracting solution is 6000～8000 ultrafilter membrane ultrafiltration through molecular cut off, and ultrafilter membrane is held back concentrated solution and carried out concentrating under reduced pressure, and directly spray drying is standby; AB-8 macroporous resin on the ultrafiltration membrane permeate liquid is used 95% ethanol elution, decompression recycling ethanol, and spray drying, standby;
(3) get Fructus Bruceae, dry and break into coarse powder, cross 50 mesh sieves, drop into the supercritical carbon dioxide extraction still, high pressure injects CO
2, extracting pressure is 25～35MPa, 50～70 ℃ of extraction temperature, CO
2Flow velocity is 1.5～2L/min, first static extracting 30～40 minutes, and again 1.5～2.5 hours dynamic extraction time, with separating still separating pressure 4.0～4.2MPa, 30～35 ℃ of separation temperatures obtain the Fructus Bruceae extract, and are standby;
(4) get Concha Arcae, Semen Descurainiae was pulverized 60 mesh sieves, added 5～8 times of water gagings, reflux, extract, 3 times, each 1～1.5 hour, merge decocting liquid, concentrate fluid extract, to get extracting solution standby for spray drying then;
(5) extracting liquorice, break into fine powder, drop into the supercritical carbon dioxide extraction still, with extracting pressure 8～10MPa, 35～45 ℃ of extraction temperature, elder generation's static extracting 1～2 hour, dynamic extraction is 5～7 hours again, separating still separating pressure 2.0～3.0Mpa, 25～35 ℃ of separation temperatures, obtain Radix Glycyrrhizae extract liquid, standby;
(6) each step of combining step (1) to (5) is extract obtained, and fully mix homogeneously promptly gets Chinese traditional compound medicine of the present invention.
As preferred version, the described desorption agent of step (1) ethanol that is concentration 65% wherein, extracting solution is the ethanol of concentration 25%, and the macroporous resin eluant is 85% ethanol, and the volume of collecting eluant is 6 times of column volumes.
As preferred version, step (2) adding concentration 5% alcoholic acid amount is 6 times of medical material amount, and the ultrafilter membrane molecular cut off is 6000.
As preferred version, the described supercritical carbon dioxide extraction pressure of step (3) is 28MPa, and extraction temperature is 55 ℃, CO
2Flow velocity is 2L/min, and the static extracting time is 30 minutes, and the dynamic extraction time is 2 hours, and the separating still separating pressure is 4.2MPa, and separation temperature is 32 ℃.
The application of Chinese traditional compound medicine provided by the invention in preparation treatment non-small cell lung cancer drug.
Chinese traditional compound medicine provided by the invention can be used for the treatment of nonsmall-cell lung cancer when making granule, capsule, tablets and other formulations medicine.
In tcm clinical practice, belong to " lump in the right hypochondrium " category according to Chinese medical theory pulmonary carcinoma.Mainly think because healthy energy is deficient, imbalance of YIN and YANG, the heresy of six climate exopathogens is taken advantage of a weak point in opponent's defence into lung, heresy stagnates in lung, causes the lungs functional disorder, lung qi stasis, mistake department falls in a surname, disorder of QI movement, and blood is capable to be obstructed, body fluid loses in defeated cloth, Tianjin is poly-to be expectorant, phlegm and qi stasis, and the resistance channels becomes silted up, so mental disorder stasis of blood poison is glued, with the passing of time form the long-pending piece of pulmonary.Therefore, pulmonary carcinoma is that a kind of whole body belongs to void, local true disease.Empty with the deficiency of YIN, deficiency of both QI and YIN is common; Actually coagulate along with the development of the course of disease shows the stagnation of QI, expectorant respectively, blood stasis, the poly-different state of poison.
Former void is basis of disease, and real is the mark of disease.So we reuse Folium Viticis Negundo, with activating QI to eliminate phlegm, eliminating phlegm and relieving asthma; With the Radix Astragali is ministerial drug, tonifying QI to produce body fluid, consolidating superficial resistance poison holding; Be aided with the Semen Lepidii (Semen Descurainiae) inducing diuresis to remove edema simultaneously, the Fructus Bruceae heat-clearing and toxic substances removing, Concha Arcae hard masses softening and resolving is at last by the Radix Glycyrrhizae coordinating the actions of various ingredients in a prescription.The dialectical tonify deficiency of we is to show consideration for the void of body, and the invigorating middle warmer band is attacked with slow local real, accomplishes whole, giving consideration to both the incidental and fundamental unified mutually with the part, so obtain good efficacy.Six-element Chinese medicine can be treated nonsmall-cell lung cancer through after the scientific composition, strengthens the patient immune function, can effectively resist nonsmall-cell lung cancer, the transfer of anticancer and diffusion hinder tumor growth, reduce the toxic and side effects of chemicotherapy, thereby improve patient's quality of life human body.
Beneficial effect: Chinese traditional compound medicine provided by the invention and preparation method thereof and prior art are now than having the following advantages:
1, Chinese traditional compound medicine provided by the invention, according to Chinese medical theory, adopt the theory of determination of treatment based on pathogenesis obtained through differentiation of symptoms and signs, formulate the present invention, show that through pharmacological evaluation and clinical experiment Chinese traditional compound medicine provided by the invention has good enhancing patient immune function through scientific composition, can effectively resist nonsmall-cell lung cancer, the transfer of anticancer and diffusion hinder tumor growth, reduce the toxic and side effects of chemicotherapy to human body, and untoward reaction is low, and is cheap.
2, the preparation method of Chinese traditional compound medicine provided by the invention, deficiency at traditional extraction separation method, physicochemical properties characteristics according to Chinese traditional compound medicine crude drug effective ingredient provided by the invention, adopt different extraction separation methods, extracts active ingredients efficient height, impurity content is low; Low effective ingredient the present invention preferably adopts the extraction of supercritical carbon dioxide extraction technology at polarity, the used organic solvent of supercritical carbon dioxide extraction technology is few, and extraction and isolating efficient height are workable, manufacturing cost is low, is fit to suitability for industrialized production.
The specific embodiment:
Further illustrate the present invention below in conjunction with specific embodiment, should understand these embodiment only is used to the present invention is described and is not used in and limit the scope of the invention, after having read the present invention, those skilled in the art all fall within the application's claims institute restricted portion to the modification of the various equivalent form of values of the present invention.
Embodiment 1:
1, the crude drug of Chinese traditional compound medicine is formed: Herba Viticis Cannabifoliae 300g, Radix Astragali 320g, Fructus Bruceae 200g, Concha Arcae 195g, Semen Descurainiae 200g, Radix Glycyrrhizae 200g.
2, the preparation method of Chinese traditional compound medicine:
(1) gets Herba Viticis Cannabifoliae 300g, break into coarse powder, add 5 times of amounts of Herba Viticis Cannabifoliae concentration, 60% ethanol, be heated to 22 ℃, soak 30min as the desorption agent; Concentration 30% alcohol reflux of 8 times of amounts of adding Herba Viticis Cannabifoliae 3 times, each 1.5 hours, merge extractive liquid, concentrated, and D101 macroporous resin on the concentrated solution is used 80% ethanol elution, decompression recycling ethanol, spray drying is standby;
(2) get Radix Astragali 320g, break into coarse powder, add concentration 5% alcoholic solution of 10 times of amounts of Milkvetch Root amount, ultrasonic extraction twice was extracted merge extractive liquid, 1.5 hours at every turn; Extracting solution is 8000 ultrafilter membrane ultrafiltration through molecular cut off, and ultrafilter membrane is held back concentrated solution and carried out concentrating under reduced pressure, and direct spray drying is standby then; AB-8 macroporous resin on the ultrafiltration membrane permeate liquid is used 95% ethanol elution, decompression recycling ethanol, and spray drying, standby;
(3) get Fructus Bruceae 200g, dry and break into coarse powder, cross 50 mesh sieves, drop into the supercritical carbon dioxide extraction still, high pressure injects CO
2, extracting pressure is 28MPa, 55 ℃ of extraction temperature, CO
2Flow velocity 2L/min, first static extracting 30 minutes, again 2 hours dynamic extraction time, with separating still separating pressure 4.2MPa, 32 ℃ of separation temperatures obtain extract, and spray drying is standby;
(4) get Concha Arcae 195g, Semen Descurainiae 200g pulverized 60 mesh sieves, added 8 times of water gagings, reflux, extract, 3 times, each 1.5 hours, merge decocting liquid, concentrate fluid extract, spray drying, standby;
(5) extracting liquorice 200g breaks into fine powder, drops into the supercritical carbon dioxide extraction still, with extracting pressure 8MPa, 35 ℃ of extraction temperature, first static extracting 1 hour, dynamic extraction is 5 hours again, with separating still separating pressure 2.0Mpa, 25 ℃ of separation temperatures obtain extracting solution, and spray drying is standby;
(6) combining step (1) is extract obtained to step (5), and fully mix homogeneously adds an amount of Icing Sugar and stabilizing agent, crosses 40 mesh sieves, and granulate promptly gets granule 1200g, and every bag of 10g of packing promptly.
Embodiment 2
1, the crude drug of Chinese traditional compound medicine is formed: Herba Viticis Cannabifoliae 330g, Radix Astragali 300g, Fructus Bruceae 180g, Concha Arcae 170g, Semen Descurainiae 160g, Radix Glycyrrhizae 200g.
2, the preparation method of Chinese traditional compound medicine:
(1) gets Herba Viticis Cannabifoliae 330g, break into coarse powder, add 8 times of amounts of Herba Viticis Cannabifoliae concentration, 70% ethanol, be heated to 30 ℃, soak 40min as the desorption agent; Concentration 30% alcohol reflux of 6 times of amounts of adding Herba Viticis Cannabifoliae 3 times, each 1.5 hours, merge extractive liquid, concentrated, and D101 macroporous resin on the concentrated solution is used 85% ethanol elution, decompression recycling ethanol, spray drying is standby;
(2) get Radix Astragali 300g, break into coarse powder, add concentration 5% alcoholic solution of 8 times of amounts of Milkvetch Root amount, ultrasonic extraction twice was extracted merge extractive liquid, 1.5 hours at every turn; Extracting solution is 6000 ultrafilter membrane ultrafiltration through molecular cut off, and ultrafilter membrane is held back concentrated solution and carried out concentrating under reduced pressure, and direct spray drying is standby then; AB-8 macroporous resin on the ultrafiltration membrane permeate liquid is used 95% ethanol elution, decompression recycling ethanol, and spray drying, standby;
(3) get Fructus Bruceae 180g, dry and break into coarse powder, cross 50 mesh sieves, drop into the supercritical carbon dioxide extraction still, high pressure injects CO
2, extracting pressure is 35MPa, 65 ℃ of extraction temperature, CO
2Flow velocity 1.8L/min, first static extracting 35 minutes, again 2.5 hours dynamic extraction time, with separating still separating pressure 4.0MPa, 35 ℃ of separation temperatures obtain extract, and spray drying is standby;
(4) get Concha Arcae 170g, Semen Descurainiae 160g pulverized 60 mesh sieves, added 6 times of water gagings, reflux, extract, 3 times, each 1.5 hours, merge decocting liquid, concentrate fluid extract, spray drying, standby;
(5) extracting liquorice 200g breaks into fine powder, drops into the supercritical carbon dioxide extraction still, with extracting pressure 10MPa, 35 ℃ of extraction temperature, first static extracting 2 hours, dynamic extraction is 6 hours again, with separating still separating pressure 2.6Mpa, 30 ℃ of separation temperatures obtain extracting solution, and spray drying is standby;
(6) combining step (1) is extract obtained to step (5), and fully mix homogeneously is crossed 60 mesh sieves, incapsulates, and every net weight 0.8g amounts to 1000, or gets said extracted thing adding corn starch, and the granulate tabletting is made tablet.
Embodiment 3
1, the crude drug of Chinese traditional compound medicine is formed: Herba Viticis Cannabifoliae 360g, Radix Astragali 285g, Fructus Bruceae 267g, Concha Arcae 186g, Semen Descurainiae 186g, Radix Glycyrrhizae 186g.
2, the preparation method of Chinese traditional compound medicine:
(1) gets Herba Viticis Cannabifoliae 360g, break into coarse powder, add 10 times of amounts of Herba Viticis Cannabifoliae concentration, 60% ethanol, be heated to 45 ℃, soak 30min as the desorption agent; Concentration 50% alcohol reflux of 8 times of amounts of adding Herba Viticis Cannabifoliae 3 times, each 1.5 hours, merge extractive liquid, concentrated, and D101 macroporous resin on the concentrated solution is used 80% ethanol elution, decompression recycling ethanol, spray drying is standby;
(2) get Radix Astragali 285g, break into coarse powder, add concentration 5% alcoholic solution of 6 times of amounts of Milkvetch Root amount, ultrasonic extraction twice was extracted merge extractive liquid, 1.5 hours at every turn; Extracting solution is 7000 ultrafilter membrane ultrafiltration through molecular cut off, and ultrafilter membrane is held back concentrated solution and carried out concentrating under reduced pressure, and direct spray drying is standby then; AB-8 macroporous resin on the ultrafiltration membrane permeate liquid is used 95% ethanol elution, decompression recycling ethanol, and spray drying, standby;
(3) get Fructus Bruceae 267g, dry and break into coarse powder, cross 50 mesh sieves, drop into the supercritical carbon dioxide extraction still, high pressure injects CO
2, extracting pressure is 30MPa, 60 ℃ of extraction temperature, CO
2Flow velocity 1.5L/min, first static extracting 30 minutes, again 2.5 hours dynamic extraction time, with separating still separating pressure 4.2MPa, 35 ℃ of separation temperatures obtain extract, and spray drying is standby;
(4) get Concha Arcae 186g, Semen Descurainiae 186g pulverized 60 mesh sieves, added 6 times of water gagings, reflux, extract, 3 times, each 1.5 hours, merge decocting liquid, concentrate fluid extract, spray drying, standby;
(5) extracting liquorice 186g breaks into fine powder, drops into the supercritical carbon dioxide extraction still, with extracting pressure 10MPa, 40 ℃ of extraction temperature, first static extracting 2 hours, dynamic extraction is 5 hours again, with separating still separating pressure 3.0Mpa, 35 ℃ of separation temperatures obtain extracting solution, and spray drying is standby;
(6) combining step (1) is extract obtained to step (5), and fully mix homogeneously is crossed 60 mesh sieves, incapsulates, and every net weight 0.8g amounts to 1200, or gets said extracted thing adding corn starch, and the granulate tabletting is made tablet.
Embodiment 4
1, the crude drug of Chinese traditional compound medicine is formed: Herba Viticis Cannabifoliae 250g, Radix Astragali 210g, Fructus Bruceae 140g, Concha Arcae 100g, Semen Descurainiae 100g, Radix Glycyrrhizae 180g
2, the preparation method of Chinese traditional compound medicine:
(1) gets Herba Viticis Cannabifoliae 250g, break into coarse powder, add 10 times of amounts of Herba Viticis Cannabifoliae concentration, 60% ethanol, be heated to 45 ℃, soak 30min as the desorption agent; Concentration 50% alcohol reflux of 8 times of amounts of adding Herba Viticis Cannabifoliae 3 times, each 1.5 hours, merge extractive liquid, concentrated, and D101 macroporous resin on the concentrated solution is used 80% ethanol elution, decompression recycling ethanol, spray drying is standby;
(2) get Radix Astragali 210g, break into coarse powder, add concentration 5% alcoholic solution of 6 times of amounts of Milkvetch Root amount, ultrasonic extraction twice was extracted merge extractive liquid, 1.5 hours at every turn; Extracting solution is 7000 ultrafilter membrane ultrafiltration through molecular cut off, and ultrafilter membrane is held back concentrated solution and carried out concentrating under reduced pressure, and direct spray drying is standby then; AB-8 macroporous resin on the ultrafiltration membrane permeate liquid is used 95% ethanol elution, decompression recycling ethanol, and spray drying, standby;
(3) get Fructus Bruceae 140g, dry and break into coarse powder, cross 50 mesh sieves, drop into the supercritical carbon dioxide extraction still, high pressure injects CO
2, extracting pressure is 30MPa, 60 ℃ of extraction temperature, CO
2Flow velocity 1.5L/min, first static extracting 30 minutes, again 2.5 hours dynamic extraction time, with separating still separating pressure 4.2MPa, 35 ℃ of separation temperatures obtain extract, and spray drying is standby;
(4) get Concha Arcae 100g, Semen Descurainiae 100g pulverized 60 mesh sieves, added 6 times of water gagings, reflux, extract, 3 times, each 1.5 hours, merge decocting liquid, concentrate fluid extract, spray drying, standby;
(5) extracting liquorice 180g breaks into fine powder, drops into the supercritical carbon dioxide extraction still, with extracting pressure 10MPa, 40 ℃ of extraction temperature, first static extracting 2 hours, dynamic extraction is 5 hours again, with separating still separating pressure 3.0Mpa, 35 ℃ of separation temperatures obtain extracting solution, and spray drying is standby;
(6) combining step (1) is extract obtained to step (5), and fully mix homogeneously is crossed 60 mesh sieves, incapsulates, and every net weight 0.8g amounts to 800, or gets said extracted thing adding corn starch, and the granulate tabletting is made tablet.
Embodiment 5
1, the crude drug of Chinese traditional compound medicine is formed: Herba Viticis Cannabifoliae 400g, Radix Astragali 400g, Fructus Bruceae 200g, Concha Arcae 200g, Semen Descurainiae 200g, Radix Glycyrrhizae 200g
2, the preparation method of Chinese traditional compound medicine:
(1) gets Herba Viticis Cannabifoliae 400g, break into coarse powder, add 10 times of amounts of Herba Viticis Cannabifoliae concentration, 60% ethanol, be heated to 45 ℃, soak 30min as the desorption agent; Concentration 50% alcohol reflux of 8 times of amounts of adding Herba Viticis Cannabifoliae 3 times, each 1.5 hours, merge extractive liquid, concentrated, and D101 macroporous resin on the concentrated solution is used 80% ethanol elution, decompression recycling ethanol, spray drying is standby;
(2) get Radix Astragali 400g, break into coarse powder, add concentration 5% alcoholic solution of 6 times of amounts of Milkvetch Root amount, ultrasonic extraction twice was extracted merge extractive liquid, 1.5 hours at every turn; Extracting solution is 7000 ultrafilter membrane ultrafiltration through molecular cut off, and ultrafilter membrane is held back concentrated solution and carried out concentrating under reduced pressure, and direct spray drying is standby then; AB-8 macroporous resin on the ultrafiltration membrane permeate liquid is used 95% ethanol elution, decompression recycling ethanol, and spray drying, standby;
(3) get Fructus Bruceae 200g, dry and break into coarse powder, cross 50 mesh sieves, drop into the supercritical carbon dioxide extraction still, high pressure injects CO
2, extracting pressure is 30MPa, 60 ℃ of extraction temperature, CO
2Flow velocity 1.5L/min, first static extracting 30 minutes, again 2.5 hours dynamic extraction time, with separating still separating pressure 4.2MPa, 35 ℃ of separation temperatures obtain extract, and spray drying is standby;
(4) get Concha Arcae 200g, Semen Descurainiae 200g pulverized 60 mesh sieves, added 6 times of water gagings, reflux, extract, 3 times, each 1.5 hours, merge decocting liquid, concentrate fluid extract, spray drying, standby;
(5) extracting liquorice 200g breaks into fine powder, drops into the supercritical carbon dioxide extraction still, with extracting pressure 10MPa, 40 ℃ of extraction temperature, first static extracting 2 hours, dynamic extraction is 5 hours again, with separating still separating pressure 3.0Mpa, 35 ℃ of separation temperatures obtain extracting solution, and spray drying is standby;
(6) combining step (1) is extract obtained to step (5), and fully mix homogeneously is crossed 60 mesh sieves, incapsulates, and every net weight 0.8g amounts to 1300, or gets said extracted thing adding corn starch, and the granulate tabletting is made tablet.
The pharmacological evaluation of embodiment 6 Chinese traditional compound medicines
1, experimental technique: get 50 of C57BL/6 mouse inbred liness, be divided into blank group at random, treatment by Chinese herbs group and western medicine group, each is organized mice and all goes down to posterity Mice Bearing Lewis Lung Cancer cell suspension 0.2mL to set up bearing mouse model in right axillary fossa subcutaneous injection, inoculate back second day, the treatment by Chinese herbs group is irritated stomach with Chinese traditional compound medicine provided by the invention, and every day 1 time, dosage is 35g/kg; The western medicine group is with cisplatin (DDP) lumbar injection every day 1 time (dosage is 1mg/kg), and blank group is irritated stomach with the normal saline of equivalent every day, and each organizes continuous 10 days.Observe mice diet and tumor growth situation every day, get blood, carry out blood cell analysis with cellanalyzer in the 11st day eyeball; Put to death mice, it is fast to peel off tumor at once, with the fast weight of electronic balance weighing tumor; Separating spleen and thymus are with electronic balance weighing spleen and thymus quality; Matched group tumor weight-herbal mixture group tumor is heavy
2, result of the test: handle the back and detect every index, wherein tumor control rate calculates as follows:
Tumor control rate=(blank group tumor weight-medicine group tumor is heavy)/blank group tumor heavy * 100%
(1) concrete outcome of the tumor weight of herbal mixture group and western medicine group and tumor control rate is as shown in table 1:
Each experimental group of table 1 is to Mice Bearing Lewis Lung Cancer cell inhibiting situation
Show by table 1 experimental result, Chinese traditional compound medicine provided by the invention has the obvious suppression effect to the Mice Bearing Lewis Lung Cancer cell, the Chinese traditional compound medicine group is compared with the blank group, mouse tumor weight has notable difference (P＜0.05～0.01), and show that by the tumor control rate experimental result Chinese traditional compound medicine provided by the invention has the effect of better inhibition Mice Bearing Lewis Lung Cancer than positive control drug cisplatin.
(2) herbal mixture group and western medicine group are to the immune organ influence of mice, and concrete experimental result is as shown in table 2:
Each experimental group of table 2 is to the situation that influences of mouse immune organ
| Number of animals | Spleen m/g | Thymus m/g | |
| Blank group | 9 | 0.1897 | 0.0543 |
| The treatment by Chinese herbs group | 19 | 0.2456 | 0.0421 |
| The western medicine group | 18 | 0.1674 | 0.0298 |
Show by table 2 experimental result; Chinese traditional compound medicine provided by the invention has the certain protection effect to the immune organ spleen of mice; the spleen of Western medicine cisplatin group treatment back mice is compared obviously the lightest with thymic weight with blank group; the Chinese traditional compound medicine group is compared with Western medicine cisplatin group; Chinese traditional compound medicine group provided by the invention has lower side effect than Western medicine cisplatin group, and Chinese traditional compound medicine quality safety provided by the invention is described.
(3) herbal mixture group and western medicine group are to the influence of the blood system of mice, and concrete experimental result is as shown in table 3:
Each experimental group of table 3 is to the situation that influences of mouse blood system
| Erythrocyte (* 10 12L -1) | Leukocyte (* 10 9L -1) | Platelet (* 10 9L -1) | Hemoglobin (g/L) | |
| Blank group | 4.01 | 14.85 | 402.33 | 81.74 |
| The treatment by Chinese herbs group | 4.43 | 13.26 | 399.51 | 88.93 |
| The western medicine group | 4.86 | 7.52 | 496.79 | 82.25 |
Show by table 3 experimental result, Chinese traditional compound medicine group provided by the invention is compared with the blank group, it is not remarkable that the RBC number of mice, leukocyte count, platelet count, hemoglobin are counted difference, and the leukocyte count of Western medicine group is starkly lower than blank group and Chinese traditional compound medicine group, illustrate that Chinese traditional compound medicine group provided by the invention is low to the side effect of the blood system of mice, the quality safety aspect is better than Western medicine cisplatin group.
In sum, Chinese traditional compound medicine provided by the invention has the obvious suppression effect to Mice Bearing Lewis Lung Cancer, is better than the Western medicine cisplatin, and untoward reaction is low, quality safety.Therefore, Chinese traditional compound medicine provided by the invention is expected to develop the anti-pulmonary carcinoma Chinese medicine preparation that becomes a new generation.
Claims (10)
1. Chinese traditional compound medicine, it is characterized in that it is made by the following raw materials by weight percent medicine: Herba Viticis Cannabifoliae 20%～40%, the Radix Astragali 20%～40%, Fructus Bruceae 10%～20%, Concha Arcae 10%～20%, Semen Descurainiae 10%～20%, Radix Glycyrrhizae 8%～20%.
2. Chinese traditional compound medicine according to claim 1, it is characterized in that it is made by the following raw materials by weight percent medicine: Herba Viticis Cannabifoliae 25%～33%, the Radix Astragali 21%～30%, Fructus Bruceae 14%～18%, Concha Arcae 10%～17%, Semen Descurainiae 10%～16%, Radix Glycyrrhizae 10%～18%.
3. Chinese traditional compound medicine according to claim 1 and 2 is characterized in that, each extracts of bulk drugs is become the medicine of granule, capsule or Tabules with pharmaceutically acceptable preparing carriers.
4. the preparation method of claim 1 or 2 described Chinese traditional compound medicines is characterized in that may further comprise the steps:
(1) get Herba Viticis Cannabifoliae, break into coarse powder, add the desorption agent, add heat soaking, add the extractant reflux, extract, 3 times, merge extractive liquid, concentrates, and the D101 macroporous resin is used ethanol elution on the concentrated solution, decompression recycling ethanol, and spray drying gets the Herba Viticis Cannabifoliae extract, and is standby; Wherein, the described desorption agent ethanol that is concentration 50%～70%;
(2) get the Radix Astragali, break into coarse powder, add 5% alcoholic solution of 5～10 times of amounts, ultrasonic extraction twice, merge extractive liquid; Extracting solution is through the ultrafilter membrane ultrafiltration, and ultrafilter membrane is held back concentrated solution and carried out the direct then spray drying of concentrating under reduced pressure, and is standby; The AB-8 macroporous resin is used ethanol elution on the ultrafiltration membrane permeate liquid, decompression recycling ethanol, and spray drying, standby;
(3) get Fructus Bruceae, dry and break into coarse powder, drop into the supercritical carbon dioxide extraction still, high pressure injects CO
2, extracting pressure is 25～35MPa, 50～70 ℃ of extraction temperature, CO
2Flow velocity is 1.5～2L/min, first static extracting 30～40 minutes, and again 1.5～2.5 hours dynamic extraction time, with separating still separating pressure 4.0～4.2MPa, 30～35 ℃ of separation temperatures obtain the Fructus Bruceae extract, and are standby;
(4) get Concha Arcae and Semen Descurainiae, pulverize, adds 5～8 times of water gagings, reflux, extract, 3 times merges decocting liquid, concentrate fluid extract, spray drying, must Concha Arcae and the Semen Descurainiae extracting solution standby;
(5) extracting liquorice, break into fine powder, drop into the supercritical carbon dioxide extraction still, with extracting pressure 8～10MPa, 35～45 ℃ of extraction temperature, elder generation's static extracting 1～2 hour, dynamic extraction is 5～7 hours again, separating still separating pressure 2.0～3.0Mpa, 25～35 ℃ of separation temperatures, obtain Radix Glycyrrhizae extract liquid, standby;
(6) combining step (1) is extract obtained to (5), abundant mix homogeneously, promptly.
5. the preparation method of Chinese traditional compound medicine according to claim 4, it is characterized in that, the described desorption agent of step (1) addition is 5～10 times of Herba Viticis Cannabifoliae medical material amount, the described extractant of step (1) is the ethanol of concentration 20%～50%, and the addition of extractant is 5～10 times of Herba Viticis Cannabifoliae medical material amount.
6. the preparation method of Chinese traditional compound medicine according to claim 4 is characterized in that, the described ultrafilter membrane molecular cut off of step (2) is 6000～8000.
7. the preparation method of Chinese traditional compound medicine according to claim 4 is characterized in that, the described supercritical carbon dioxide extraction pressure of step (3) is 28MPa, and extraction temperature is 55 ℃, CO
2Flow velocity is 2L/min, and the static extracting time is 30 minutes, and the dynamic extraction time is 2 hours, and separation temperature is 32 ℃.
8. the preparation method of Chinese traditional compound medicine according to claim 4 is characterized in that, the time of the each reflux, extract, of step (4) is 1～1.5 hour.
9. claim 1 or the 2 described Chinese traditional compound medicines application in preparation treatment non-small cell lung cancer drug.
10. the application of the described Chinese traditional compound medicine of claim 3 in preparation treatment non-small cell lung cancer drug.
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| 付金荣.卵巢囊肿的中医药治疗现状.《中医文献杂志》.2007,(第3期), * |
| 张洪亮.食管癌.《新疆中医药》.2006,第24卷(第5期), * |
| 杨小红.原发性肝癌的中医研究概况.《安徽中医临床杂志》.2002,第14卷(第3期), * |
| 林宗广.活血化瘀法治疗原发性肝癌的研究现状与进展.《肿瘤》.1998,第18卷(第3期), * |
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