CN104825784B - A kind of Chinese medicine composition with anti-gastric cancer activity and its preparation method and application - Google Patents
A kind of Chinese medicine composition with anti-gastric cancer activity and its preparation method and application Download PDFInfo
- Publication number
- CN104825784B CN104825784B CN201510252875.5A CN201510252875A CN104825784B CN 104825784 B CN104825784 B CN 104825784B CN 201510252875 A CN201510252875 A CN 201510252875A CN 104825784 B CN104825784 B CN 104825784B
- Authority
- CN
- China
- Prior art keywords
- parts
- chinese medicine
- gastric cancer
- medicine composition
- cancer activity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The invention discloses a kind of Chinese medicine composition with anti-gastric cancer activity and preparation method thereof and its application, the Chinese medicine composition is by 15~45 parts of RADIX CODONOPSIS PILOSULAE from Shanxi of China, 10~30 parts of the tuber of dwarf lilyturf, 20~60 parts of oldenlandia diffusa, 15~45 parts of hairyvein agrimony, 15~45 parts of salvia chinensis, 10 ~ 30 parts of rhizoma pinellinae praeparata are made.Chinese medicine composition provided by the invention with anti-gastric cancer activity, proportioning is scientific and reasonable, and medication is rigorous, has anticancer removing toxic substances, dissipating phlegm and removing blood stasis, QI invigorating and stomach yin-nourishing and other effects.Test result indicates that having strong killing action to stomach cancer cell, and the generation of TNF can be promoted, improve tumor patient immunity, and it is safe, toxic side effect is low.Preparation method provided by the invention, steady quality, effective component extracting content is high, workable, is adapted to industrialized production.
Description
Technical field
The present invention relates to a kind of Chinese medicine, and in particular to a kind of Chinese medicine composition with anti-gastric cancer activity and preparation method thereof
And application.
Background technology
Tumour has turned into the number one killer for 21 century threatening human survival, has more than 600 ten thousand people to die from every year on average various swollen
Knurl disease.China is one of world's cancer country occurred frequently, and is presented a rapidly rising trend every year.
With environmental pollution getting worse, pollution of waterhead, food pollution, and as people's diet is irregular, diet is various
Change, differenceization, easily get a cancer of the stomach.The medicine that clinically treatment tumour includes stomach cancer at present is mainly chemicals, but due to chemotherapy
Drug toxicity is big, the also human autoimmune such as the kill leucocyte of non-selectivity, lymphocyte while tumour cell is killed
Cell, cause patient usually because because autoimmunity inferior capabilities are complicated by infection, patient uses erious adverse reaction, and the course for the treatment of is grown,
It is costly, there is larger limitation in Clinical practice.
Traditional Chinese medicine has unique advantage in terms of tumour is treated, and the natural class medicine using Chinese medicine as representative has poison is secondary to make
With the features such as small, action target spot is various, better tolerance, more and more it is taken seriously in terms of tumour is treated.But report at present
Anti-gastric cancer medicament dosage is larger, and impurity component is more, works slower, to stomach and a kind of burden, is unfavorable for curing stomach cancer.
Therefore, it is good to research and develop a kind of antitumous effect, dosage is few, has good therapeutic effect to stomach cancer, and
The low antineoplastic Chinese traditional medicine of adverse reaction is significant.
The content of the invention
Goal of the invention：Present invention aims to solve the deficiencies of the prior art, and provides a kind of one kind proportioning it is scientific and reasonable, curative effect is true
Cut, Clinical practice safety, the low Chinese medicine composition with anti-gastric cancer activity of adverse reaction, another object of the present invention is to provide
This has the preparation method of the Chinese medicine composition of anti-gastric cancer activity and its is preparing the application in treating stomach cancer medicine.
Technical scheme：In order to realize the above object the technical scheme that the present invention takes is：
A kind of Chinese medicine composition with anti-gastric cancer activity, it is made up of the bulk drug of following parts by weight：
15~45 parts of RADIX CODONOPSIS PILOSULAE from Shanxi of China, 10~30 parts of the tuber of dwarf lilyturf, 20~60 parts of oldenlandia diffusa, 15~45 parts of hairyvein agrimony, salvia chinensis
15~45 parts, 10~30 parts of rhizoma pinellinae praeparata.
Preferably, the above-described Chinese medicine composition with anti-gastric cancer activity, it is by following parts by weight
Bulk drug is made：15~30 parts of RADIX CODONOPSIS PILOSULAE from Shanxi of China, 10~20 parts of the tuber of dwarf lilyturf, 20~40 parts of oldenlandia diffusa, 15~30 parts of hairyvein agrimony, stone is shown in
15~30 parts are worn, 10~20 parts of rhizoma pinellinae praeparata.
Preferably, the above-described Chinese medicine composition with anti-gastric cancer activity, it is by following parts by weight
Bulk drug is made：15 parts of RADIX CODONOPSIS PILOSULAE from Shanxi of China, 10 parts of the tuber of dwarf lilyturf, 20 parts of oldenlandia diffusa, 15 parts of hairyvein agrimony, 15 parts of salvia chinensis, rhizoma pinellinae praeparata 10
Part.
Preferably, the above-described Chinese medicine composition with anti-gastric cancer activity, by the extract of each bulk drug
The medicine of oral liquid, granule, capsule or Tabules is prepared into pharmaceutically acceptable carrier.
The preparation method of Chinese medicine composition of the present invention with anti-gastric cancer activity, it comprises the following steps：
(1) RADIX CODONOPSIS PILOSULAE from Shanxi of China, the tuber of dwarf lilyturf, oldenlandia diffusa, hairyvein agrimony, salvia chinensis and rhizoma pinellinae praeparata are taken in parts by weight, add total medicine
50~80% ethanol of 6~15 times of volumes of material weight, backflow or continuous circumfluence extraction 2~3 times, 1~3 hour every time, close
And extract solution, it is concentrated under reduced pressure under 50~70 degrees Celsius, obtains large pore resin absorption column on concentrate, be first eluted with water, then successively
It is 20%~80% ethanol elution with volumetric concentration；The ethanol eluate for merging that volumetric concentration is 60~80% is collected, is concentrated to give
Extract；
(2) extract that step (1) obtains and pharmaceutically acceptable carrier is taken to be prepared into oral liquid, granule, capsule
Agent or the medicine of Tabules.
Preferably, the preparation method of the above-described Chinese medicine composition with anti-gastric cancer activity, step (1) institute
The macroporous absorbent resin stated is D101 resins.
Application of the Chinese medicine composition of the present invention with anti-gastric cancer activity in anti-gastric cancer medicament is prepared.
When the extract of each raw material of Chinese medicine composition provided by the invention with anti-gastric cancer activity is made into oral liquid,
Add distilled water to dissolve the extract of each raw material, filter, add syrup, adjust pH value, oral liquid is made.
When the extract of each raw material of Chinese medicine composition provided by the invention with anti-gastric cancer activity is made into tablet,
The extract and lactose or cornstarch of each raw material, it is necessary to when add magnesium stearate lubricant, be well mixed, whole grain, then press
Tablet is made in piece.
Handle when capsule is made in the extract of each raw material of Chinese medicine composition provided by the invention with anti-gastric cancer activity
The extract and carrier lactose or cornstarch of each raw material are well mixed, and whole grain is then encapsulated that capsule is made.
When granule is made in the extract of each raw material of Chinese medicine composition provided by the invention with anti-gastric cancer activity,
The extract and diluent lactose or cornstarch of each raw material are well mixed, whole grain, dry, granule is made.
Chinese medicine composition provided by the invention with anti-gastric cancer activity, RADIX CODONOPSIS PILOSULAE from Shanxi of China is sweet, mild-natured；Returns spleen, lung channel；Matter is moistened
Gas and, there is strengthening the spleen and tonifying the lung, nourishing qi and blood is promoted the production of body fluid, and cures mainly weakness of the spleen and the stomach, anorexia and loose stool, lassitude hypodynamia, and the deficiency syndrome of the lung is breathed with cough, and can be strengthened
Passive protective physical fitness, reduce blood pressure, blood-nourishing, tonifying middle-Jiao and Qi, treatment gastric ulcer.
The tuber of dwarf lilyturf sweet slight bitter, tremble with fear, enter lung channel, stomach, the heart channel of Hang-Shaoyin.With Yin nourishing and lung moistening, relieving restlessness that clears away heart-fire, reinforcing stomach reg fluid effect, tool
There is treatment dryness of the lung dry cough, spit blood, spitting of blood, consumptive lung disease, lung carbuncle, consumptive disease dysphoria with smothery sensation, quench one's thirst, pyreticosis Tianjin wound, dry throat, constipation effect.
Oldenlandia diffusa bitter sweet, tremble with fear.Enter the heart channel of Hang-Shaoyin, Liver Channel, the spleen channel.With clearing heat and detoxicating；Dampness removing acts on, and has treatment lung heat
Breath with cough；Abscess of throat；Acute appendicitis；Furuncle sore；Venomous snake bite；Puckery pain of heat gonorrhea；Oedema；Dysentery；Enteritis；Jaundice with damp-heat pathogen；Cancerous swelling work(
Effect.
Hairyvein agrimony nature and flavor：It is bitter, puckery, it is mild-natured.Channel tropism：Lung channel；Liver Channel；The spleen channel.With astringing to arrest bleeding, stop dysentery, efficiently.It is main
Control：Spitting of blood；Spit blood；Hematuria；Have blood in stool；Dysentery；Traumatic bleeding.
The salvia chinensis property of medicine：It is bitter, pungent, it is mild-natured, enter Liver Channel.The effect of with promoting blood circulation and removing blood stasis, clearing heat and detoxicating, swelling and pain relieving.
Rhizoma pinellinae praeparata nature and flavor：It is pungent, warm.Channel tropism：Returns spleen, stomach, lung channel.Effect：It is eliminating dampness and eliminating phlegm.Cure mainly：For dry throat, phlegm
More cough and asthmas, phlegm retention anti-dazzle nervous, anemophlegmatic vertigo, phlegm and headache.
The present invention, using diagnosis and treatment, flavour of a drug composition, all medicines of the above is filtered out by many experiments according to traditional Chinese medical theory
Share with good anticancer removing toxic substances, dissipating phlegm and removing blood stasis, supplementing qi and nourishing yin, there is good prevention effect to stomach cancer.
Beneficial effect：Chinese medicine composition provided by the invention with anti-gastric cancer activity is compared to the prior art with following
Advantage：
1st, for the present invention according to traditional Chinese medical theory, theoretical using diagnosis and treatment carries out Chinese prescription screening, there is provided have
The Chinese medicine composition proportioning of anti-gastric cancer activity is scientific and reasonable, has the work(such as good anticancer removing toxic substances, dissipating phlegm and removing blood stasis, supplementing qi and nourishing yin
Effect.Test result indicates that the Chinese medicine composition provided by the invention with anti-gastric cancer activity suppresses stomach cancer cell with good
Growth and the effect that gastric cancer tumor Apoptosis can be promoted.
2nd, test result indicates that the Chinese medicine composition provided by the invention with anti-gastric cancer activity with righting reinforcing and is exempted from
Epidemic disease function facilitation, and TNF (INF) generation can be promoted, Mutiple Targets are played, multiple organ treats the work of tumour
With, tumor patient pain can be reduced, extends life cycle, improves the effect of life in patients, and show through long-term experiment research,
Chinese medicine composition provided by the invention with anti-gastric cancer activity is safe, and toxic side effect is low.
3rd, it is provided by the invention with anti-gastric cancer activity Chinese medicine composition extract can conveniently and pharmaceutical carriers system
It is standby into a variety of formulations, it is clinically convenient to take；The preparation of Chinese medicine composition simultaneously provided by the invention with anti-gastric cancer activity
Method, it is workable, design extraction and purification process, obtained extraction especially in accordance with each herbal medicine active ingredient chemical property
Thing active constituent content is high, and impurity is few, and has the advantages that absorption is fast, can play drug effect rapidly, and clinically dosage is few, and work
Skill is stable, is adapted to industrialized production.
Embodiment：
With reference to specific embodiment, the present invention is furture elucidated, it should be understood that these embodiments be merely to illustrate the present invention and
Limitation the scope of the present invention is not used in, after the present invention has been read, various of equal value shapes of the those skilled in the art to the present invention
The modification of formula falls within the application appended claims limited range.
Implementation column 1
1. a kind of Chinese medicine composition with anti-gastric cancer activity, it is made up of the bulk drug of following parts by weight：RADIX CODONOPSIS PILOSULAE from Shanxi of China
15 grams, 10 grams of the tuber of dwarf lilyturf, 20 grams of oldenlandia diffusa, 15 grams of hairyvein agrimony, 15 grams of salvia chinensis, 10 grams of rhizoma pinellinae praeparata.
2. the preparation method of the Chinese medicine composition of the present invention with anti-gastric cancer activity, it comprises the following steps：
(1) 15 grams of RADIX CODONOPSIS PILOSULAE from Shanxi of China, 10 grams of the tuber of dwarf lilyturf, 20 grams of oldenlandia diffusa, 15 grams of hairyvein agrimony, salvia chinensis 15 are taken in parts by weight
Gram, 10 grams of rhizoma pinellinae praeparata adds 60% ethanol (volumetric concentration) of total 12 times of volumes of medicinal material weight, continuous circumfluence extraction 3 times, often
Secondary 2 hours, merge extract solution, be concentrated under reduced pressure under 50~70 degrees Celsius, obtain large pore resin absorption column on concentrate, be first washed with water
It is de-, with volumetric concentration it is successively then 20%~80% ethanol elution；The ethanol that merging volumetric concentration is 60~80% is collected to wash
De- liquid, is concentrated to give extract；
(2) extract for taking step (1) to obtain, lactose is added, cornstarch is well mixed, and adds lubricant stearic acid
Magnesium, it is well mixed, tablet is made in whole grain, then tabletting.(lot number：001)
Implementation column 2
1. a kind of Chinese medicine composition with anti-gastric cancer activity, it is made up of the bulk drug of following parts by weight：RADIX CODONOPSIS PILOSULAE from Shanxi of China
30 grams, 20 grams of the tuber of dwarf lilyturf, 40 grams of oldenlandia diffusa, 30 grams of hairyvein agrimony, 30 grams of salvia chinensis, 20 grams of rhizoma pinellinae praeparata.
2. the preparation method of the Chinese medicine composition of the present invention with anti-gastric cancer activity, it comprises the following steps：
(1) 30 grams of RADIX CODONOPSIS PILOSULAE from Shanxi of China, 20 grams of the tuber of dwarf lilyturf, 40 grams of oldenlandia diffusa, 30 grams of hairyvein agrimony, salvia chinensis 30 are taken in parts by weight
Gram, 20 grams of rhizoma pinellinae praeparata adds 80% ethanol (volumetric concentration) of total 10 times of volumes of medicinal material weight, refluxing extraction 2 times, and every time 3
Hour, merge extract solution, be concentrated under reduced pressure under 50~70 degrees Celsius, obtain large pore resin absorption column on concentrate, be first eluted with water, so
With volumetric concentration it is successively afterwards 20%~80% ethanol elution；The ethanol eluate for merging that volumetric concentration is 60~80% is collected,
It is concentrated to give extract；
(2) extract for taking step (1) to obtain, then add diluent lactose or cornstarch is well mixed, whole grain, do
It is dry, granule is made.(lot number：002)
Implementation column 3
1. a kind of Chinese medicine composition with anti-gastric cancer activity, it is made up of the bulk drug of following parts by weight：RADIX CODONOPSIS PILOSULAE from Shanxi of China
45 grams, 30 grams of the tuber of dwarf lilyturf, 60 grams of oldenlandia diffusa, 45 grams of hairyvein agrimony, 45 grams of salvia chinensis, 30 grams of rhizoma pinellinae praeparata.
2. the preparation method of the Chinese medicine composition of the present invention with anti-gastric cancer activity, it comprises the following steps：
(1) 45 grams of RADIX CODONOPSIS PILOSULAE from Shanxi of China, 30 grams of the tuber of dwarf lilyturf, 60 grams of oldenlandia diffusa, 45 grams of hairyvein agrimony, salvia chinensis 45 are taken in parts by weight
Gram, 30 grams of rhizoma pinellinae praeparata, 70% ethanol (volumetric concentration) of total 8 times of volumes of medicinal material weight is added, refluxing extraction 2 times, 2 is small every time
When, merge extract solution, be concentrated under reduced pressure under 50~70 degrees Celsius, obtain large pore resin absorption column on concentrate, be first eluted with water, then
It is successively 20%~80% ethanol elution with volumetric concentration；The ethanol eluate for merging that volumetric concentration is 60~80% is collected, it is dense
Contract to obtain extract；
(2) extract for taking step (1) to obtain, then and lactose, cornstarch mixing with whole grain is then encapsulated
Capsule is made.(lot number：003)
The anti-gastric cancer experimental study of implementation column 4
(1) to gastric carcinoma cells (MGC in nude mouse-803) cell inhibitory action
Experimental method：By the MGC of adherent growth in Tissue Culture Flask-803Cell, is prepared into cell suspension, and adjustment cell is dense
Spend, counted under mirror, in every mouse right fore armpit subcutaneous vaccination 5 × 106Individual MGC-803Cell, 24 are inoculated with altogether, is randomly divided into
Treatment group (Chinese medicine composition provided by the invention with antitumor activity, lot number 001, gastric infusion 3g/kg), and blank group
(waiting capacity physiological saline), every group 10.After inoculation 48 hours, medication, continuous 21 days, after drug withdrawal, animal observation is put to death
MGC-803Cell growing state and claims knurl weight in nude mouse.Specific experiment the results are shown in Table 1.
Table 1 is to MGC in nude mouse-803The inhibitory action of cell
By more than test result indicates that：Chinese medicine composition provided by the invention with anti-gastric cancer activity is to human gastric cancer nude mice
Transplanting has obvious inhibitory action in vivo, show in the Chinese medicine composition body active with anti-gastric cancer provided by the invention with
The effect of very strong anti-gastric cancer, it can be used for the treatment of stomach cancer.
Implementation column 5 has experiment of the Chinese medicine composition of anti-gastric cancer activity to immunoregulation effect
(1) to the influence of mouse IgA, IgG, IgM content
Experimental method：Take ICR small white mouses 36,18~22g of body weight, be randomly divided into Normal group, lotus knurl control group and
Treatment group, traditional vaccination stomach cancer cell, 24 hours after inoculation, treatment group, which gavages the present invention, has the Chinese traditional medicine composition of anti-gastric cancer activity
Thing (lot number 001) 0.3ml/ only (2g/kg), take a blood sample within the 15th day by small white mouse eyeball.Determine IgA, IgG, IgM content, specific experiment
It the results are shown in Table 2.
Influence (mg/dl of the table 2 to mouse IgA, IgG, IgM content)
*P<0.01 (compared with Normal group) * * P<0.05 (compared with lotus knurl control group)
By with upper table 2 test result indicates that：The mouse of stomach cancer is inoculated with, Immunoglobulin IgA, IgG, IgM content are obvious
Less than normal control mice；The present invention have anti-gastric cancer activity Chinese medicine composition can improve the low IgA of mouse,
IgG, IgM content, show the Chinese medicine composition provided by the invention with anti-gastric cancer activity with immunologic enhancement, Ke Yiti
The high tumor patient immunologic function of itself, so as to be advantageous to improve Quality of Life of Tumor Patients and promote itself antitumor action.
(2) the raising effect induced to TNF (INF)
Experimental method：Wistar big white mouse 24 are taken, are randomly divided into control group and treatment group, every animal is injected intraperitoneally
10mg BCG vaccines, the μ gLPS of intraperitoneal injection in the 15th day 80, take a blood sample after 90 minutes.Treatment group gavages the present invention, and there is anti-gastric cancer to live
Property Chinese medicine composition 2ml/ only (lot number 001,2g/kg), continuous 14 days, control group such as gavaged at the capacity physiological saline.
Test serum is taken, is diluted to various concentrations；Target cell L929 is adjusted with 10% calf serum, RPM1-1640 nutrient solutions
Whole is 2 × 106/ml cells, is added in 96 porocyte culture plates；100 μ l/ holes, then add the test serum 100 of different extension rates
μ l/ holes；37 DEG C are put, 5%CO2Cultivated 18 hours in incubator.Result judgement：With the maximum dilution multiple of 50% target cell death
For INF potency.Concrete outcome is shown in Table 3.
The facilitation that table 3 induces to rat INF
*P<0.001 (compared with Normal group)
By table 3 test result indicates that：Chinese medicine composition provided by the invention with anti-gastric cancer activity can be obviously promoted
INF's induces, (P<0.001), illustrate that the Chinese medicine composition provided by the invention with anti-gastric cancer activity can pass through induced tumors
Necrosin INF reaches antitumor purpose.
Test result indicates that, the Chinese medicine composition provided by the invention with anti-gastric cancer activity is to patients with gastric cancer more than
Immunologic function has raising effect, and can promote TNF (INF) generation, can strengthen the antitumor work(of patient itself
Energy.
Embodiment 6
Chinese medicine composition clinical test results provided by the invention with anti-gastric cancer activity are shown：Choose clinical definite
Patients with gastric cancer 46, the Chinese medicine composition (embodiment of the present invention 1) provided by the present invention with anti-gastric cancer activity is taken, through 2
Clinical observation in individual month, take the clinics such as the tumor patient of Traditional Chinese medicine compound composition provided by the invention, stomach pain, indigestion
Symptom, which has, to be obviously improved, and quality of life in patients with gastric is improved.Show it is provided by the present invention with anti-gastric cancer activity
Chinese medicine composition has the function that to protect to immunologic function and improvement, and efficient clinic observation is 85.7%.
The present invention carries out proportioning prescription, medication is rigorous, passes through the above according to traditional Chinese medical theory using the theory of diagnosis and treatment
Test result indicates that the Chinese medicine composition provided by the invention with anti-gastric cancer activity is with anticancer removing toxic substances, dissipating phlegm and removing blood stasis, QI invigorating
With stomach yin-nourishing and other effects, have it is obvious suppress the growth and transferance of gastric cancer tumor, and carried without obvious toxic-side effects, the present invention
What is supplied there is the Chinese medicine composition of anti-gastric cancer activity can be used for the medicine for preparing anti-gastric cancer.
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (7)
1. a kind of Chinese medicine composition with anti-gastric cancer activity, it is characterised in that it is made up of the bulk drug of following parts by weight：
15~45 parts of RADIX CODONOPSIS PILOSULAE from Shanxi of China, 10~30 parts of the tuber of dwarf lilyturf, 20~60 parts of oldenlandia diffusa, 15~45 parts of hairyvein agrimony, salvia chinensis 15~
45 parts, 10 ~ 30 parts of rhizoma pinellinae praeparata.
2. the Chinese medicine composition according to claim 1 with anti-gastric cancer activity, it is characterised in that it is by following parts by weight
Several bulk drugs are made：15~30 parts of RADIX CODONOPSIS PILOSULAE from Shanxi of China, 10~20 parts of the tuber of dwarf lilyturf, 20~40 parts of oldenlandia diffusa, 15~30 parts of hairyvein agrimony,
15~30 parts of salvia chinensis, 10 ~ 20 parts of rhizoma pinellinae praeparata.
3. the Chinese medicine composition according to claim 2 with anti-gastric cancer activity, it is characterised in that it is by following parts by weight
Several bulk drugs are made：15 parts of RADIX CODONOPSIS PILOSULAE from Shanxi of China, 10 parts of the tuber of dwarf lilyturf, 20 parts of oldenlandia diffusa, 15 parts of hairyvein agrimony, 15 parts of salvia chinensis, method half
10 parts of summer.
4. the Chinese medicine composition with anti-gastric cancer activity according to any one of claims 1 to 3, it is characterised in that will be each
The extract and pharmaceutically acceptable carrier of bulk drug are prepared into the medicine of oral liquid, granule, capsule or Tabules.
5. the preparation method of the Chinese medicine composition with anti-gastric cancer activity described in any one of claims 1 to 3, it is characterised in that
Comprise the following steps：
(1) parts by weight as described in any one of claims 1 to 3 take RADIX CODONOPSIS PILOSULAE from Shanxi of China, the tuber of dwarf lilyturf, oldenlandia diffusa, hairyvein agrimony, and stone is shown in
Wear and rhizoma pinellinae praeparata, add 50~80% ethanol of total 6~15 times of volumes of medicinal material weight, backflow or continuous circumfluence extraction 2~3
It is secondary, 1 ~ 3 hour every time, merge extract solution, be concentrated under reduced pressure under 50~70 degrees Celsius, obtain large pore resin absorption column on concentrate, first
It is eluted with water, with volumetric concentration is successively then 20%~80% ethanol elution；Collect the ethanol for merging that volumetric concentration is 60~80%
Eluent, it is concentrated to give extract；
(2) take extract that step (1) obtains and pharmaceutically acceptable carrier be prepared into oral liquid, granule, capsule or
The medicine of Tabules.
6. the preparation method of the Chinese medicine composition with anti-gastric cancer activity described in claim 5, it is characterised in that step（1）
Described macroporous absorbent resin is D101 resins.
7. there is the Chinese medicine composition of anti-gastric cancer activity in anti-gastric cancer medicament is prepared described in any one of claims 1 to 3
Using.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510252875.5A CN104825784B (en) | 2015-05-18 | 2015-05-18 | A kind of Chinese medicine composition with anti-gastric cancer activity and its preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510252875.5A CN104825784B (en) | 2015-05-18 | 2015-05-18 | A kind of Chinese medicine composition with anti-gastric cancer activity and its preparation method and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104825784A CN104825784A (en) | 2015-08-12 |
| CN104825784B true CN104825784B (en) | 2018-04-03 |
Family
ID=53804231
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510252875.5A Active CN104825784B (en) | 2015-05-18 | 2015-05-18 | A kind of Chinese medicine composition with anti-gastric cancer activity and its preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104825784B (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1824156A (en) * | 2005-12-31 | 2006-08-30 | 罗先乐 | Chinese medicine mixed preparation for treating stomach precancer and its preparation method |
| CN101204538A (en) * | 2006-12-20 | 2008-06-25 | 上海林静医疗器械有限公司 | Chinese traditional medicine preparation for stomach malignancy |
| CN103800799A (en) * | 2014-01-21 | 2014-05-21 | 张传志 | Traditional Chinese medicine for treating stomach cancer |
-
2015
- 2015-05-18 CN CN201510252875.5A patent/CN104825784B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1824156A (en) * | 2005-12-31 | 2006-08-30 | 罗先乐 | Chinese medicine mixed preparation for treating stomach precancer and its preparation method |
| CN101204538A (en) * | 2006-12-20 | 2008-06-25 | 上海林静医疗器械有限公司 | Chinese traditional medicine preparation for stomach malignancy |
| CN103800799A (en) * | 2014-01-21 | 2014-05-21 | 张传志 | Traditional Chinese medicine for treating stomach cancer |
Non-Patent Citations (2)
| Title |
|---|
| 基于数据挖掘的周仲瑛教授治疗胃癌病艾回顾性研究;李柳;《中国优秀硕士学位论文全文数据库 医药卫生科技辑》;20110430;19-20页表格及第一、第30页第2.段 * |
| 消癌解毒方药效活性物质基础概述;孙东东等;《中华中医药杂志》;20150331;第30卷(第3期);第803页左栏第2段 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104825784A (en) | 2015-08-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103768534B (en) | A kind of Chinese medicine composition with antitumor activity | |
| KR20110132407A (en) | Plant extract compositions for prevention and treatment of influenza | |
| CN1879868A (en) | Medicine for treating dysmenorrhea and its preparing method | |
| CN104857435B (en) | A kind of Traditional Chinese medicine compound composition with antitumor activity and its preparation method and application | |
| CN101904974B (en) | Chinese medicinal composition for treating malignant tumor diseases and preparation method thereof | |
| CN101850032B (en) | Anti-tumor traditional Chinese medicine composition and preparation method and application thereof | |
| CN101711848B (en) | Chinese medicinal composition capable of adjunctively treating tumour | |
| CN104857436B (en) | Traditional Chinese medicine compound composition with resisting liver cancer activity and its preparation method and application | |
| CN104825783B (en) | Chinese medicine composition with antitumor activity and its preparation method and application | |
| CN104825784B (en) | A kind of Chinese medicine composition with anti-gastric cancer activity and its preparation method and application | |
| CN107149653A (en) | A kind of Traditional Chinese medicine compound composition with antitumor activity and its preparation method and application | |
| CN104840748B (en) | Chinese medicine composition with anti-cancer of the brain activity and its preparation method and application | |
| CN104840747B (en) | Chinese medicine composition with antithyroid cancer activity and its preparation method and application | |
| CN104840746B (en) | A kind of Chinese medicine composition with anti-lung cancer activity and its preparation method and application | |
| CN105125639A (en) | Traditional Chinese medicine composition for preventing and treating mastitis of dairy cattle | |
| CN102949681B (en) | Composition for preventing or treating colds, and its preparation method | |
| CN105267946A (en) | Hirudo small peptide medicine for treating ischemic cerebral apoplexy | |
| CN104825922A (en) | Traditional Chinese medicine composition with anti-colorectal cancer activity and preparation method and application of traditional Chinese medicine composition | |
| CN1899385A (en) | Process for preparing Chinese medicine compound injection for treating chronic renal failure and use | |
| CN104840727B (en) | Chinese medicine composition with anti-cancer of the esophagus activity and its preparation method and application | |
| CN104840716B (en) | Traditional Chinese medicine compound composition with anti-breast cancer activity and its preparation method and application | |
| CN102274428A (en) | Pharmaceutical composition with effect on treating irritable bowel syndrome and preparation method and application thereof | |
| CN1316989C (en) | Capsule with dried human placemta and red sage root and its preparing process | |
| CN1256976C (en) | Anti-cancer Chinese medicinal composition and its preparing method | |
| CN1245204C (en) | Medicine for curing acute pharyngitis and its preparing method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |