A kind of immunity enhancement type nutrition meals and preparation method thereof
Technical field
The present invention relates to a kind of immunity enhancement type nutrition meals and preparation method thereof, these nutrition meals are as the functional food with immunoregulation effect.
Background technology
Black-food is the agricultural resource of China's tradition dietotherapeutic, and since ancient times, just there is the saying of " meeting black must the benefit " in China.The record of Shennong's Herbal and Compendium of Material Medica, resource has liver-kidney tonifying for black rice, black soybean etc. " black five cereals ", the beauty treatment of invigorating blood circulation, control weakness, effect such as delay senility.Modern scientific research shows that also the trophic function of food and its natural colour are closely related.Black five cereals resource contains rich in protein, amino acid, crude fat, vitamin B1, vitamin B2, vitamin E, phosphorus, calcium, iron, zinc and cellulose, exceeds 30%~50% than common five cereals.And its amino acid A wide selection of colours and designs, the essential amino acid ratio is superior to bread and cheese, and the proportioning ratio is reasonable, more approaches human amino acid's pattern.The melanin of black-food belongs to flavonoids anthocyanin compounds; Contain physiological activators such as flavonol, anthocyanidin, alkaloid, phytosterol, cardiac glycoside, saponin(e, tannin; Thereby have some health care, delay senility, improve effects such as alimentary anemia, enhance immunity power, antitumor action, reducing blood lipid and prevention coronary heart disease as removing free radical.
One of typical fruit tree in many tropical and subtropical countries that longan is unique flavor, be of high nutritive value is regarded as excellent tonic product always.China Ming Dynasty Li Shizhen (1518-1593 A.D.) once had the evaluation of " money benefit be good with the longan " to longan in Compendium of Material Medica." the southern regions of the Yunnan Province book on Chinese herbal medicine " claimed longan: " beneficial spleen whets the appetite for nourishing blood and tranquilization, long intelligence arrest sweating ".Longan fruit is nutritious, comprises glucose, sucrose, acids, and other has protein, fat, Cobastab, P, C etc., is praised highly to be " southern genseng ".
In the prior art, above-mentioned raw materials basically all is single edible, does not process the functional food of instant edible, has restricted the further development and use of above-mentioned nutrient raw material.
Summary of the invention
The purpose of this invention is to provide a kind of immunity enhancement type nutrition meals and preparation method thereof with immunoregulation effect.
The objective of the invention is to realize through following technical measures: a kind of immunity enhancement type nutrition meals, it comprises following percentage by weight raw material:
Black five cereals swelling powder: 10~60%
Sesame oil: 5~20%
Bean powder: 10~30%
Icing Sugar: 5%~20%
Longan powder: 1~10%
Dextrin: 5~20%
Lucidum spore powder: 1~5%.
For increasing percent protein, the present invention also comprises following percentage by weight raw material:
Glutamine peptide: 1~10%
Hydrolyzing lactoalbumin: 5~15%.
Harmonious for satisfying product amino acid, improve absorptivity, the present invention also comprises following percentage by weight raw material:
Taurine: 0.01~0.05%
Arginine: 0.01~0.05%.
The invention also discloses the preparation method of above-mentioned immunity enhancement type nutrition meals, its step comprises:
[1] preparation of black five cereals swelling powder: the ratio than 1: 3: 1 takes by weighing black rice husk, black rice, black soya bean and mixes by weight, adds mixing after the entry than 10: 0.5~1 by weight again; Expanded under 150~200 ℃ of conditions with single serew bulker; Be crushed to 60 orders then;
[2] preparation of sesame oil: frying Semen sesami nigrum at first fries with Semen sesami nigrum and to produce sesame fragrance degree of being; Mix frying Semen sesami nigrum and peanut oil in 2: 1~2 weight portion ratio then, process sesame oil with colloid mill;
[3] preparation of longan powder: commercially available dried longan, 50~70 ℃ of dryings were crushed to 60 orders with pulverizer after 1~5 hour;
[4] batching: take by weighing black five cereals swelling powder, sesame oil, bean powder, dextrin, Icing Sugar, longan powder, lucidum spore powder by the percentage by weight of above-mentioned each component and mix;
[5] pack: packing into by specification is fit to seal after the inner packing, reinstalls external packing and is finished product.
Also can add glutamine peptide, hydrolyzing lactoalbumin, taurine, arginine mixing by the percentage by weight of above-mentioned each component evenly in the above-mentioned blending process of the present invention.
The present invention sterilizes after to above-mentioned finished product packing: open magnetron 4-6 (each 700W), line speed 15-25r/min, 65~75 ℃ of product final temperatures.
Nutrition meals of the present invention are easy to use, quick, and the consumer is accepted.The immunity enhancement type nutrition meals that adopt raw materials such as black rice, black soybean, Semen sesami nigrum and longan to adopt modern food processing technology to be made; Delicate mouthfeel and have peat-reek; Contain multiple functional components simultaneously to the human body beneficial; Can be used as leisure food, also can be used as the dietary supplements of enhancing body immunity with excellent health functions.
For proving above-mentioned effect, immunity enhancement type nutrition meals of the present invention have been carried out the test of pesticide effectiveness, specific as follows:
1, experimental condition
1.1 sample
Experiment adopts nutrition meals human body to recommend a day dosing 120g/60 (kgbw), and (wrap every days 3; Every bag 40g product) and 10 multiple doses that upwards are pushed into 240g/60 (kgbw), 360g/60 (kgbw) as receiving amount of reagent; Establish 3 altogether and receive the amount of reagent group, 20g/kgbw, 40g/kgbw, 60g/kgbw.The immunity enhancement type nutrition meals that the present invention makes are added into basal feed, add proportioning of the present invention and are respectively: 10%, 20%, 30%.
1.2 animal used as test
The KM mouse, male, body weight 18-22g, SPF level.
1.3 grouping and processing
(1) divides into groups
Animal is quarantined under laboratory condition behind the 7d, at random 60 mouse is divided into 5 groups, receives the amount of reagent group except that 3; In addition intraperitoneal injection of cyclophosphamide (the physiological saline preparation, once a day, each 50mg/kg; Continuous three days) cause the immunocompromised model group, and establish the normal control group.Every group 12.
(2) handle
10g/ only gave animal subject and was tried thing every day, gave the appearance amount according to diet increase and decrease adjustment slightly weekly.Each is organized mouse and freely ingests, drinks water, continuously 30d.
Blank group: the basal feed of ingesting;
Endoxan model group: the basal feed of ingesting;
Receive the amount of reagent group: basic, normal, high dose groups nutrition every day meals intake is respectively 20g/kgbw, 40g/kgbw, 60g/kgbw;
1.4 data analysis
Adopt SPSS to carry out data analysis.
2, result of the test
The mouse spleen lymphocyte transformant competence for added value that immunity enhancement type nutrition meals are induced experiment mice immune organ internal organs/body weight ratio, ConA, serum HD50 value (HC
50), the influence of monokaryon-macrophage phagocytic exponential sum NK cytoactive is as shown in the table:
Table 1 nutrition meals are to the influence (
n=12) of mouse immune organ index
A representes to compare with control group p<0.05; B representes to compare with control group p<0.01; Δ is represented to compare p<0.01 with the endoxan group;
n=12)
The mouse spleen lymphocyte conversion reaction that table 2 nutrition meals are induced ConA and the influence (
n=12) of NK cytoactive
A representes to compare with control group p<0.05; B representes to compare with control group p<0.01; Δ is represented to compare p<0.01 with the endoxan group;
Table 3 nutrition meals are cleaned up phagocytic function and HD50 value (HC to mouse carbon
50) influence
N=12)
A representes to compare with control group p<0.05; B representes to compare with control group p<0.01; Δ is represented to compare p<0.01 with the endoxan group;
Result of the test shows:
(1) each dose groups of immunity enhancement type nutrition meals all can increase the mouse body weight; And 20g/kgd dose groups and 40g/kgd dose groups spleen weight and body weight ratio and thymic weight and body weight ratio all are higher than control group (p<0.05), and the 60g/kgd dose groups is compared difference not statistically significant (P>0.05) with control group.Significant difference (P<0.01) is arranged between normal control group and each given the test agent group and model group.
(2) nutrition meals 20g/kgd and 20g/kgd dose groups can be significantly or utmost point conspicuousness rising LSI, and 60g/kgd dose groups and normal control group not statistically significant relatively.Each dose groups of the meals of nutrition simultaneously all can promote the NK cells in mice activity, and middle dose groups has been compared significant difference (p<0.05) with control group.
(3) phagocytic index of 20g/kgd dose groups and 40g/kgd dose groups mouse all is higher than control group in monokaryon-macrophage function detects, and wherein the 40g/kgd dose groups has been compared obvious significant difference (p<0.05) with control group.Each dose groups mice serum HD50 value HC in the humoral immune function experiment
50All be higher than the contrast class value, wherein 20g/kgd and 40g/kgd dose groups and control group all have significantly or utmost point significant difference (p<0.05, P<0.01).Prompting 20g/kgd and 40g/kgd dosage nutrition meals have the ability that mouse produces serum hemolysin that strengthens.
To sum up; After giving 3 kinds of dosage 30d of nutrition meals to mouse, serum hemolysin is measured the result and is shown, institute put into trial operation and supports meals when the dosage of 20g/kgbw, 40g/kgbw (being equivalent to 10 and 20 times of human body recommendation absorptions); Can promote the generation of mouse internal antibody, improve humoral immune function.The mouse spleen lymphocyte transformation experiment presentation of results that ConA induces, under this experiment condition, put into trial operation and support meals when 20g/kgbw, 40g/kgbw dosage, can promote the mouse cell immunologic function.NK cells in mice activity experiment presentation of results, nutrition meals have the effect that strengthens the NK cells in mice activity when 20g/kgbw, 40g/kgbw dosage.These presentation of results immunity enhancement type nutrition meals have certain immunological enhancement to normal mouse.
The specific embodiment
Embodiment one
[1] take by weighing 20 parts black rice husk, 60 parts of black rices, 20 parts of black soya beans mixing, stir behind the water than 5 parts of addings by weight again, expanded with single serew bulker under 150 ℃ of conditions, be crushed to 60 orders then, be black five cereals swelling powder;
[2] the frying Semen sesami nigrum is to produce sesame fragrance degree of being; 2: 1 by weight mixed frying Semen sesami nigrum and peanut oil are processed sesame oil with colloid mill then;
[3] commercially available dried longan, 50 ℃ of dryings were crushed to 60 orders with pulverizer after 5 hours;
[4] take by weighing 40% black five cereals swelling powder by weight percentage, 10% sesame oil, 15% bean powder, 20% dextrin, 6% Icing Sugar, 6% longan powder, 3% lucidum spore powder mixes;
[5] pack into by specification and be fit to seal after the inner packing, reinstall external packing and be finished product.
Embodiment two
Present embodiment and embodiment one are basic identical, be not both in blending process also can add glutamine peptide, and hydrolyzing lactoalbumin, the percentage by weight proportioning of concrete each component:
[4] take by weighing 40% black five cereals swelling powder by weight percentage, 10% sesame oil, 15% bean powder, 20% dextrin, 5% Icing Sugar, 3% longan powder, 1% lucidum spore powder, 1% glutamine peptide, 5% hydrolyzing lactoalbumin mixes.
Embodiment three
Present embodiment and embodiment one are basic identical, are not both in blending process also can add taurine, and arginine mixes evenly; The percentage by weight proportioning of concrete each component:
[4] take by weighing 39.98% black five cereals swelling powder by weight percentage, 15% sesame oil, 15% bean powder, 20% dextrin, 5% Icing Sugar, 3% longan powder, 2% lucidum spore powder, 0.01% taurine, 0.01% arginine mixes evenly.
Embodiment four
[1] take by weighing 20 parts black rice husk, 60 parts of black rices, 20 parts of black soya beans mixing, stir behind the water than 8 parts of addings by weight again, expanded with single serew bulker under 160 ℃ of conditions, be crushed to 60 orders then, be black five cereals swelling powder;
[2] the frying Semen sesami nigrum is to produce sesame fragrance degree of being; 2: 1.5 by weight mixed frying Semen sesami nigrum and peanut oil are processed sesame oil with colloid mill then;
[3] commercially available dried longan, 60 ℃ of dryings were crushed to 60 orders with pulverizer after 4 hours;
[4] take by weighing 26.9% black five cereals swelling powder, 10% sesame oil, 20% bean powder, 10% dextrin by weight percentage; 10% Icing Sugar, 5% longan powder, 3% lucidum spore powder, 5% glutamine peptide; 10% hydrolyzing lactoalbumin, 0.05% taurine, 0.05% arginine mix evenly;
[5] pack into by specification and be fit to seal after the inner packing, reinstall external packing and be finished product.
[6] sterilization: open 4 of magnetrons (each 700W), line speed 20r/min, 67 ℃ of product final temperatures.
Embodiment five
[1] take by weighing 20 parts black rice husk, 60 parts of black rices, 20 parts of black soya beans mixing, stir behind the water than 10 parts of addings by weight again, expanded with single serew bulker under 180 ℃ of conditions, be crushed to 60 orders then, be black five cereals swelling powder;
[2] the frying Semen sesami nigrum is to produce sesame fragrance degree of being; 2: 2 by weight mixed frying Semen sesami nigrum and peanut oil are processed sesame oil with colloid mill then;
[3] longan powder: commercially available dried longan, 65 ℃ of dryings were crushed to 60 orders with pulverizer after 3 hours;
[4] take by weighing 16.9% black five cereals swelling powder, 12% sesame oil, 25% bean powder, 5% dextrin by weight percentage; 12% Icing Sugar, 8% longan powder, 5% lucidum spore powder, 8% glutamine peptide; 8% hydrolyzing lactoalbumin, 0.05% taurine, 0.05% arginine mix evenly;
[5] pack into by specification and be fit to seal after the inner packing, reinstall external packing and be finished product.
[6] sterilization: open 6 of magnetrons (each 700W), line speed 22r/min, 73 ℃ of product final temperatures.
The foregoing description only is used to set forth the present invention, does not limit protection scope of the present invention.Each constitutive material black five cereals swelling powder, sesame oil, longan powder in the foregoing description prepare the gained, and other raw material all adopts commercially available ready-made raw material.Those skilled in the art all can realize the object of the invention according to above scope of disclosure.