CN102008524A - Application of volatile oil of foeniculum vulgare Mill. to preparation of medicament for preventing postoperative abdominal adhesion - Google Patents
Application of volatile oil of foeniculum vulgare Mill. to preparation of medicament for preventing postoperative abdominal adhesion Download PDFInfo
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Abstract
The invention discloses the application of volatile oil of foeniculum vulgare Mill. to the preparation of a medicament for preventing postoperative abdominal adhesion. In the invention, an intestinal adhesion animal model is established and interference is produced by the volatile oil of foeniculum vulgare Mill. to prove that the volatile oil of foeniculum vulgare Mill. can function as the medicament for preventing the postoperative abdominal adhesion.
Description
Technical field
The present invention relates to medicine and health product technology field, relate to the application that a kind of known Fructus Foeniculi volatile oil is used to prepare prevention of postoperative abdominal adhesions medicine.
Background technology
Abdominal adhesions is one of major complications of abdominal postoperative, and in clinical practice, the incidence rate of abdominal postoperative abdominal adhesions is up to 90%, about 5% sticking together property of patient intestinal obstruction; Adhesion is a kind of reparation reaction of human body to damage and ischemic tissue, be a kind of fibroplasia inflammatory reaction of body, but excessively the adhesion meeting causes severe complication---intestinal obstruction.The method and the measure that prevent the abdominal post-operation abdominal adhesions at present are numerous, but abdominal adhesions and intestinal obstruction still happens occasionally, and develop new control abdominal adhesions medicine and have the important clinical meaning.
Fructus Foeniculi is the dry mature fruit of samphire Fructus Foeniculi Foeniculum vulgare Mill..You Ming Huai perfume (or spice) " the fragrant son of property of medicine opinion 》 ， Huai " Newly Revised Canon of Materia Medica ", aniseed " Kaibao Bencao ", Fructus Foeniculi, cumin " Bencao Tujing ", wild Fructus Foeniculi " carrying out Cliff rock book on Chinese herbal medicine ", Fructus Anisi Stellati " Zhu Shi collection proved recipe ", Gu Miaoxiang, Gu Xiang " modern practical Chinese medicine ", fragrant son " Chinese medicinal plants will ".Dispersing cold for relieving pain is arranged, regulate the flow of vital energy and in effect.
The composition of Fructus Foeniculi volatile oil is very complicated, and main component is trans-anethole, is limonene secondly, fenchone, other have estragole, γ-Song Youxi, australene, myrcene, nopinene, Camphora, lauro lene, the sabinene of methoxyphenyl acetone and trace, α-phellandrene, cymol, 1, the 8-cineole, the 4-terpineol, trans-the fenchol acetas, anisaldehyde etc.16 kinds of fatty acids through identifying are formed to be had: 10-octadecenoic acid, arachidic acid, Palmic acid, mountain Yu acid, myristic acid, stearic acid, lauric acid, 15 carbonic acid, two hendecoic acids etc.Fruit also contains stigmasterol, and umbelliferone is by Palmic acid, arachidic acid, behenic acid and the wax mixture that higher alcohol became greater than 18 carbon, cupreol, xanthotoxin, α-Amyrin imperatorin, bergapten and baelfruit element.
Summary of the invention
The objective of the invention is to, provide Fructus Foeniculi volatile oil to be used to prepare the application of prevention of postoperative abdominal adhesions medicine, the applicant gives the Fructus Foeniculi volatile oil intervention by setting up the intestinal adhesion animal model, has proved that Fructus Foeniculi volatile oil can be as prevention of postoperative abdominal adhesions medicine.These medicines can be external application, oral or the injection various dosage forms.
The specific embodiment
Existing document has proved that Fructus Foeniculi volatile oil is a kind of safe material, and the inventor provides following correlation test.
One, inquires into the preventive effect of Fructus Foeniculi volatile oil to the postoperative abdominal adhesions
Abdominal adhesions is one of major complications of abdominal postoperative, documents and materials
[1]The incidence rate that shows the abdominal postoperative abdominal adhesions is up to 90%, about 5% sticking together property of patient intestinal obstruction
[2]Adhesion is a kind of reparation reaction of human body to damage and ischemic tissue, be a kind of fibroplasia inflammatory reaction of body, but excessively the adhesion meeting causes severe complication---intestinal obstruction.The method and the measure that prevent the abdominal post-operation abdominal adhesions at present are numerous, but abdominal adhesions and intestinal obstruction still happens occasionally, and develop new control abdominal adhesions medicine and have the important clinical meaning.
The Fructus Foeniculi external application umbilical part treatment abdominal post-operation flatulence of propagandizing hotly commonly used clinically
[3], reduce intestinal obstruction.Fructus Foeniculi contains more volatile oil, and its main body composition is trans-anethole, estragole, fenchone and limonene composition
[4], heating back volatile oil very easily discharges, and causes and is filling the air fragrance in the air.Infer in view of the above and think that above-mentioned mechanism is that heating impels Fructus Foeniculi volatile oil to discharge, and goes in the body by Transdermal absorption, reduces intestinal adhesion and blocks.For this reason, this experiment gives the Fructus Foeniculi volatile oil intervention by setting up the intestinal adhesion animal model, and can inquire into Fructus Foeniculi volatile oil the prevention of postoperative abdominal adhesions.
1, material
1.1 animal
66 of healthy ♂ SD rats, body constitution amount (210 ± 10) g is provided by The Fourth Military Medical University of P.L.A's Experimental Animal Center.
1.2 medicine
(the synchronous way of distillation of steam is extracted quintessence oil in the preparation of Fructus Foeniculi (Yuquan, Gansu, the place of production) volatile oil
[4]): with standard water steam distillation unit continuous still 500g Fructus Foeniculi coarse powder 5h, reuse normal hexane liquid is used anhydrous Na after extracting 5 times
2SO
4Drying, unnecessary solvent is removed in distillation, obtains 13.5g Fructus Foeniculi volatile oil (productive rate is 2.7%).
2, method
2.1 animal grouping
At random 66 rats are divided into: sham operated rats (10), operative control group (14) and (Fructus Foeniculi) volatile oil external application group (14), volatile oil are irritated stomach group (14), volatile oil lumbar injection group (14); Water is can't help in the 12h fasting before the art.
2.2 model preparation
Operative control group: 2% pentobarbital sodium (30mgkg
-1) intraperitoneal injection of anesthesia, dorsal position is rat fixedly, and conventional preserved skin, sterilization, shop aseptic towel are successively gone into abdomen in the hypogastric region center, and otch is about 2cm, seeks to the light and slow proposition of caecum, and to petechial hemorrhage, area is 2cm * 1cm with dry sterile gauze friction caecum appendix portion.Scratch with the ophthalmology tweezer then scrape, mosquito forceps clamp left side stomach wall each 5 times, form to promote adhesion, avoid injuring its hetero-organization simultaneously as far as possible.Behind caecum Hui Na abdominal cavity, No. 0 silk thread successively closes abdomen.Sham operated rats is opened not process behind the abdomen and is just closed abdomen; Each group of volatile oil is handled same operative control group, the back volatile oil external application group that finishes umbilical external application (50mgkg
-1, 3 d
-1), volatile oil irritates stomach group (30mgkg
-1, 3 d
-1), volatile oil lumbar injection group (25mgkg
-1, 3 d
-1).Five groups of rats are respectively put to death half respectively at postoperative 7d and 14d.
2.3 adhesion grading evaluation
5 grades of classification method according to Phillips
[5]: 0 grade, the serosal surface reparation is good, and no adhesion was counted 0 fen; The I level, the appendix cecum through rubbing and the adhesion area of surrounding tissue account for percentage ratio＜20% through the appendix cecum serosal surface area of friction, and no adhesive band forms, and counts 1 fen; The II level though the adhesion area percentage between above-mentioned position is 20% ~ 40% or area＜20%, has 1 adhesive band, counts 2 fens; The III level though the adhesion area percentage between above-mentioned position is 40%～60% or area＜40%, has 2 adhesive bands, counts 3 fens; The IV level though adhesion area percentage 〉=60% between above-mentioned position or area＜60% have 3 and above adhesive band or caecum and the direct adhesion of stomach wall, was counted 4 fens.
2.4 statistical procedures
All data are used SPSS12.0 statistical softwares and are handled, the result with mean ± standard deviation (
The F check is adopted in ± s) expression,
P＜0.05 has statistical significance for difference.
3, result
It is poor that postoperative 12h respectively organizes the rat general state, movable few, and 3 processed group idols of sham operated rats and volatile oil have into water, food, the operation group food of not intaking; Postoperative 24h operation group rat general state is poor, and is movable few, advances low amounts of water and food, increases before other two groups of activities, advances more water and food; Postoperative 48h respectively organize the rat general state still can, increase before the activity, drinking-water and feed are substantially near normally; Each treated animal general state of postoperative 72h, activity, drinking-water and feed are normal substantially.
Sham operated rats does not have adhesion, and caecum appendix cecum does not have and thickens.The treated caecum of operative control group thickens distortion, and much thicker the adhesive band that forms with surrounding tissue is, needs the separation of sharp property, and abdominal adhesions all appears in 14 rats, and wherein 10 rats have formed III level and above adhesion.Through 3 accidental thickening of processed group caecum of Fructus Foeniculi volatile oil, the adhesive band of formation is tiny, and passivity is promptly separable, and the adhesion area is little, does not have III level and above adhesion.The scoring of each processed group 7d of operative control group and volatile oil and 14d adhesion degree is all apparently higher than sham operated rats, difference have statistical significance (
P＜0.01); The scoring of each processed group 7d of volatile oil and 14d adhesion degree all is starkly lower than operative control group, difference have statistical significance (
P＜0.01).
Appraisal result sees the following form.
5 groups of rat abdominal cavity adhesion grading grade forms
Annotate: compare # with sham operated rats
P＜0.01; Compare with operative control group, ●
P＜0.01.
3, discuss
Abdominal adhesions depends on fibrinous formation.Operation is early stage, and gastrointestinal motility slows down, and defensive inflammation reaction takes place for impaired, the ischemia of local organization, peritoneum, and inflammatory mediators such as tumor necrosis factor, interleukin increase, and cause the fibrinolytic effect to be suppressed, and fibrin increases in the extracellular precipitation, and adhesion organization forms
[6-8]The scoring of each processed group 7d of volatile oil and 14d adhesion degree all be starkly lower than operative control group (
P＜0.01), and do not have the above adhesion of III level, illustrate that Fructus Foeniculi volatile oil has preventive effect to the postoperative abdominal adhesions.Fructus Foeniculi volatile oil can significantly prevent the rat abdominal cavity adhesion to form, and reduces the adhesion degree.
Fructus Foeniculi is the dry mature fruit of samphire Fructus Foeniculi, nature and flavor suffering, temperature, return taste Liver and kidney warp, has dispersing cold for relieving pain, the regulating qi-flowing for harmonizing stomach effect can be transferred smooth gastrointestinal mechanism of qi, coordinates the taste lifting, reach therapeutic purposes to recover the taste elevating function, be used for colic of cold type stomachache, abdominal distention, lack of appetite and vomiting
[9], be usually used in treating the gastrointestinal motility disorder disease clinically.
Pharmacology of Chinese materia medica
[10]Think that hot flavor Chinese medicine can be regulated the gastrointestinal smooth myokinesis, and the rheology of the blood flow of improvement, antithrombotic effect are arranged, also have anti-inflammatory, suppress the outgrowth effect of tissue abnormalities; Mostly be plant volatile oil, glycoside, alkaloid with the closely-related material of suffering flavor effect, for Fructus Foeniculi volatile oil prevention of postoperative abdominal adhesions provides the pharmacology foundation.
Studies show that
[11-12], Fructus Foeniculi can reduce the secretion of hepatic fibrosis rats tumor necrosis factor, suppresses the rat liver inflammation, reduces hepatic fibrosis rats collagen fiber precipitation, and tangible effect of anti hepatic fibrosis is arranged; Infer that in view of the above the mechanism of Fructus Foeniculi volatile oil prevention of postoperative abdominal adhesions may be to reduce inflammatory mediator release, it is relevant in the extracellular precipitation to reduce fibrin, and concrete mechanism remains further to be studied.
Studies show that
[13]The Fructus Foeniculi volatile oil main component has short oozes effect, illustrates that its easy Transdermal absorption goes in the body prevention of postoperative abdominal adhesions by Transdermal absorption.In view of the above the Fructus Foeniculi volatile oil that extracts is made appropriate dosage forms, will not only make things convenient for the patient to use but also reduce the medical material waste.
List of references:
【1】Scot-Coombes?D，Whawell?S，Vipond?MN，et?al.Human?in?tralperitoneal?fibrinolytic response?to?elective?surgery[J].Br?J?Surg.?1995，82（3）：414。
[2] Wang Jifu, gastroenterological surgery [M], Beijing: People's Health Publisher, 2000:512-520.
[3] Yang Xiuping, the xeothermic deposited control postoperative abdominal distention of Fructus Foeniculi, the Guangxi Chinese medicine, 2009,32(4): 44.
[4] He Jinming, Xiao Yanhui, Wu Jingling, concocting method is to the influence of Fructus Foeniculi quintessence oil extraction ratio and component ratio thereof, the time precious traditional Chinese medical science traditional Chinese medicines, 2008,19(11): 2598-2600.
【5】Phillips?RK，Dudley?HA.The?effect?of?tetracycline?lavage?and?trauma?on?visceral?and?parietal?peritoneal?ultrastructure?and?adhesion?formation.Br?J?Surg，1984，71（7）:537-539。
【6】Vural?B，Cantiirk?NZ，Esen?N，et?a1.The?role?of?neutrophils?in?the?formation?of peritoneal?adhesions[J].Hum?Reprod，1999，14（1）：49。
[7] Zhang Ping, Du Xiaohong, Yang Bin, pla-pcl isolating membrane prevent the research [J] of rat postoperative abdominal adhesions effect, general outer basis of China and clinical magazine, 2006:13(2): 194.
【8】Kossi?J，Salminen?P，Rantala?A，et?a1.?Population-based?study?of?the?surgical?workload?and?economic?impact?of?bowel?obstruction?caused?by?postoperative?adhesions[J].Br?J?Surg，2003，90（11）:144。
[9] Chinese Pharmacopoeia Commission, Pharmacopoeia of People's Republic of China (an one) [M], Beijing: Chinese Medicine science and technology publishing house, 2010:44-45.
[10] Xu Xiaoyu, pharmacology of Chinese materia medica [M], the 1st edition, Beijing: People's Health Publisher, 2005:5-6.
[11] Liu Yuping, Xu Yan, Gan Ziming etc., Fructus Foeniculi be to the influence [J] of experimental hepatic fibrosis rats cytokine TNF-α, Xinjiang Medicine University's journal, and 2008,31(4): 427-429.
[12] Wu Xiaochuan, Wang Xinxing, Ma Xiumin etc., Uigurs medicine Fructus Foeniculi be to the influence of hepatic fibrosis rats collagen fiber, Chinese national folk medicine, and 2009,18(17): 143-146.
[13] Shen Qi, Xu Lianying, Fructus Foeniculi is studied the short effect of oozing of 5-fluorouracil, Chinese patent medicine, 2001,23(7): 469-470.
Two, the mechanism of Fructus Foeniculi hot compress umbilical part treatment flatulence
Purpose: the mechanism of research Fructus Foeniculi hot compress umbilical part treatment flatulence provides scientific basis for instructing clinical rational drug use.
Fructus Foeniculi is the dry mature fruit of samphire Fructus Foeniculi, often propagandizes back external application umbilical part treatment flatulence clinically hotly
[1]Fructus Foeniculi contains more quintessence oil, and its main body composition is trans-anethole, estragole and fenchone, limonene composition
[2], heating back quintessence oil very easily discharges, and causes and is filling the air fragrance in the air.Infer in view of the above and think that the mechanism of Fructus Foeniculi hot compress umbilical part treatment flatulence is: 1, heating impels Fructus Foeniculi volatile oil to discharge, and goes in the body by Transdermal absorption, brings into play drug effect by improving gastrointestinal motility disorder; 2, the direct effect of heat.The applicant has set up mice gastrointestinal motility disorder model for this reason, and with Fructus Foeniculi volatile oil and heat intervention, observes its effect.
1, material
Animal: cleaning level Kunming mouse, body constitution amount (20 ± 2) g, The Fourth Military Medical University of P.L.A's animal center provides.
Medicine and reagent: (Pharmaceutical Xinzheng, Tianjin limited company, lot number: 0912104), (the synchronous way of distillation of steam is extracted quintessence oil to Fructus Foeniculi (Yuquan, Gansu, the place of production) quintessence oil to atropine sulfate injection
[3]), blue dextran 2000(Sweden Pharmacia company produces, and is made into 20mg/ml solution with deionized water).
Instrument: dual-beam ultraviolet-uisible spectrophotometer (production of UV4501 Tianjin Gangdong development in science and technology company limited), desk centrifuge (production of TGL-18C-C Shanghai Chu Bai laboratory equlpment company limited).
Hot compress bag (self-control): melted paraffin wax is packed into and is made plastic packets (1 ㎝ * 1 ㎝) by oneself;
Water deposited bag (self-control): 20 ℃ of water are packed into and are made plastic packets (1 ㎝ * 1 ㎝) by oneself.
2, method:
24 male mice adaptabilities are fed 3d, and all be divided into 3 groups at random: water applies matched group, paraffin hot compress group, Fructus Foeniculi volatile oil group.
(1) water bag matched group: 20 ℃ of water bags are the mice umbilical part fixedly, each 30min;
(2) paraffin hot compress group: 45 ℃ of paraffin bags of beginning temperature are the mice umbilical part fixedly, each 30min;
(3) Fructus Foeniculi volatile oil group: mice umbilical external application Fructus Foeniculi volatile oil (50mg/20g).2 times/d, continuous 3d, water 18h is can't help in fasting, operate 1h for the last time on the 4th day after, lumbar injection atropine sulfate 1 ㎎/㎏, blue dextran 2000 solution (20mg/m1) 0.4ml that gives behind the 20min to have configured irritates stomach, the cervical vertebra execution that dislocates behind the 20min.Cut open the belly rapidly and get full stomach, its content is dissolved in the 4ml distilled water, the centrifugal 10min of 3000r/min, get supernatant and record absorbance with spectrophotometer in λ 620nm place, other gets the same concentration blue dextran 2000 solution 0.4ml of a test tube, and is centrifugal under the similarity condition, get supernatant and record absorbance, as standard pipe, by formula: (the residual pigment absorbance of 1-gastric/standard pipe pigment absorbance) * 100%, calculate as gastric emptying rate.Simultaneously getting sphincter of pylorus to the full intestinal of distal colon rapidly places on the glass plate of normal saline moistening, peel off gently, do not add any pull strength and make its tiling, measuring mice sphincter of pylorus place to pigment rapidly advances front end distance and sphincter of pylorus place to the full intestinal distance of distal colon, by formula: the full intestinal length of enteral advance distance (cm) ÷ (cm) * 100% is calculated as intestinal propelling rate.
1.3 statistical procedures:
Each organize data all with (
) expression, carrying out date processing with the SPSS12.0 statistical software, one factor analysis of variance compares between the employing group in twos,
P＜0.05 has statistical significance for difference.
2, result
Compare with water bag matched group and paraffin hot compress group, Fructus Foeniculi volatile oil group gastric emptying rate and intestinal propelling rate increase,
P＜0.01, statistical significance is arranged, paraffin hot compress group and water bag matched group compare, and gastric emptying rate and intestinal propelling rate slightly increase,
P＞0.05, do not have statistical significance.The results are shown in Table 1.
Table 1: mice gastric emptying rate and the comparison of intestinal propulsion rate (
, n=8)
Annotate: with water bag matched group and paraffin hot compress group ratio, #
P＜0.01.
3, discuss
Pharmacology of Chinese materia medica
[3]Think that hot flavor Chinese medicine can be regulated the gastrointestinal smooth myokinesis, the effect of its relieving distension by promoting circulation of QI and the adjusting of gastrointestinal smooth muscle have substantial connection; Fructus Foeniculi nature and flavor suffering, temperature are returned taste Liver and kidney warp, have dispersing cold for relieving pain, and the regulating qi-flowing for harmonizing stomach effect is used for colic of cold type stomachache, abdominal distention, lack of appetite and vomiting
[4], be usually used in treating the gastrointestinal motility disorder disease clinically.
The gastrointestinal motility disorder disease belongs to the traditional Chinese medical science " epigastric fullness " category, with abdominal part feeling of fullness distending pain and feel sick, vomiting, belch are not relaxed etc. is main clinical manifestation.Pathogeny is many is retarded by silt by gastrointestinal, functional activity of QI being not smooth, so that due to the taste lifting mistake department.Modern medicine thinks that the gastrointestinal motility disorder disease is relevant with multiple factor, and its main pathophysiological basis is delayed gastric emptying and small intestinal transfer function obstacle.
Fructus Foeniculi has dispersing cold for relieving pain, and the regulating qi-flowing for harmonizing stomach effect has the smooth gastrointestinal mechanism of qi of accent, coordinates the taste lifting, reaches therapeutic purposes to recover the taste elevating function.This experiment finds, Fructus Foeniculi volatile oil the gastric emptying of gastrointestinal motility disorder animal due to the atropine and intestinal advanced have facilitation (
P＜0.01), heat (temperature) advances influence little to gastric emptying and intestinal, and this is insensitive relevant to caloric stimulation with visceroceptor
[5]Studies confirm that the excitatory neurotransmitter acetylcholine produces excitable activity at digestive tract, promote gastrointestinal smooth muscle to shrink
[5], and atropine is a kind of antagonist of non-selective M cholinoceptor, can the irritability of gastrointestinal smooth muscle be descended by suppressing the effect of acetylcholine, reduces the amplitude and the frequency of wriggling.So Fructus Foeniculi volatile oil may be by the antagonism atropine to the antagonism of M cholinoceptor, increase the secretion of gastrointestinal tract excitatory neurotransmitter acetylcholine, thereby promote gastrointestinal motility, exact mechanism still remains further research.
This experiment shows that the Fructus Foeniculi volatile oil main component has the short effect of oozing
[7], proved that further its easy Transdermal absorption goes in the body.
List of references:
[1] Yang Xiuping, the xeothermic deposited control postoperative abdominal distention of Fructus Foeniculi, the Guangxi Chinese medicine, 2009,32(4): 44.
[2] He Jinming, Xiao Yanhui, Wu Jingling. concocting method is to the influence of Fructus Foeniculi quintessence oil extraction ratio and component ratio thereof, the time precious traditional Chinese medical science traditional Chinese medicines, 2008,19(11): 2598-2600.
[3] Xu Xiaoyu, pharmacology of Chinese materia medica: the modern times understanding of flavour of a drug, the 1st edition. Beijing: People's Health Publisher, 2005:6.
[4] Chinese Pharmacopoeia Commission. Pharmacopoeia of People's Republic of China (an one), Beijing: Chinese Medicine science and technology publishing house, 2010:44-45.
[5] Zhu Changgeng, Li Yunqing, Gu Xiaosong, neuroanatomy, the 2nd edition. Beijing: People's Health Publisher, 2009:710,218,747.
[6] Shen Qi, Xu Lianying, Fructus Foeniculi is studied the short effect of oozing of 5-fluorouracil, Chinese patent medicine, 2001,23(7): 469-470.
Three, Fructus Foeniculi volatile oil is to the influence of gastrointestinal motility disorder mice gastrointestinal motility
Purpose: observe Fructus Foeniculi volatile oil and water extract (deoiling) cause gastrointestinal motility disorder mice gastrointestinal motility to atropine influence.
Fructus Foeniculi is the dry mature fruit of samphire Fructus Foeniculi, and nature and flavor suffering, temperature are returned taste Liver and kidney warp, have dispersing cold for relieving pain, and the regulating qi-flowing for harmonizing stomach effect is used for colic of cold type stomachache, abdominal distention, lack of appetite and vomiting
[1], be usually used in gastrointestinal dysfunction aspect disease clinically.This experiment causes the influence of gastrointestinal motility disorder mice gastrointestinal motility by inquiring into the different extracts of Fructus Foeniculi to atropine, tentatively illustrates the main effective ingredient of its treatment gastrointestinal dysfunction.
, material
Animal: cleaning level Kunming mouse, body constitution amount (20 ± 2) g, The Fourth Military Medical University of P.L.A's animal center provides.
Medicine and reagent: (Pharmaceutical Xinzheng, Tianjin limited company, lot number: 091214), (the synchronous way of distillation of steam is extracted quintessence oil to Fructus Foeniculi (Yuquan, Gansu, the place of production) quintessence oil to atropine sulfate injection
[2]), Fructus Foeniculi water extract (the water extract simmer down to contains crude drug 75mg/ml after removing quintessence oil), (Chengdu Kanghong Medicine Group Co.ltd produces Chinese holly edge acid mosapride dispersible tablet, lot number: 100610), blue dextran 2000(Sweden Pharmacia company produces, and is made into 20mg/ml solution with deionized water).
Instrument: dual-beam ultraviolet-uisible spectrophotometer (production of UV4501 Tianjin Gangdong development in science and technology company limited), desk centrifuge (production of TGL-18C-C Shanghai Chu Bai laboratory equlpment company limited).
, method:
50 male mices all are divided into 5 groups at random: blank group, atropine model group, water extract+atropine group, Fructus Foeniculi volatile oil+atropine group, mosapride+atropine group.The administration of weighing behind the mice adaptability nursing 3d is tested: blank group, atropine model group are all to normal saline, press 0.2ml/10g and irritate stomach, water extract+atropine group irritates stomach for the Fructus Foeniculi water extract (containing Fructus Foeniculi 75mg/m1) that deoils, press 0.2ml/10g and irritate stomach, Fructus Foeniculi volatile oil+atropine group is irritated stomach with the 300mg/kg quintessence oil, and mosapride+atropine group irritates stomach for mosapride suspension (containing mosapride 15mg/m1).The mice dosage is calculated as follows
[3]: the dosage of mice (mg/kg)=9.10 * people's dosage (mg/kg).1 time/d, behind the continuous use 3d, fasting (can't help water, medicine) 18h, behind the 4th day normal gastric infusion 1h, the isometric normal saline of blank group lumbar injection, other organizes equal lumbar injection atropine sulfate 1mg/kg, and blue dextran (BD) 2000 solution (20mg/m1) 0.4ml that gives behind the 20min to have configured irritates stomach, and the cervical vertebra dislocation is put to death behind the 20min.Cut open the belly rapidly and get full stomach, its content is dissolved in the 4ml distilled water, the centrifugal 10min of 3000r/min, get supernatant and record absorbance in λ 620nm place with spectrophotometer, other gets the same concentration blue dextran 2000 solution 0.4ml of a test tube, and is centrifugal under the similarity condition, gets supernatant and records absorbance, as standard pipe, by formula: (the residual pigment absorbance of 1-gastric/standard pipe pigment absorbance) * 100% calculates as gastric emptying rate.Simultaneously getting sphincter of pylorus to the full intestinal of distal colon rapidly places on the glass plate of normal saline moistening, peel off gently, do not add any pull strength and make its tiling, measuring mice sphincter of pylorus place to pigment rapidly advances front end distance and sphincter of pylorus place to the full intestinal distance of distal colon, by formula: the full intestinal length of enteral advance distance (cm) ÷ (cm) * 100% is calculated as intestinal propelling rate.Carry out date processing with the SPSS12.0 statistical software, each organize data all with (
) expression, one factor analysis of variance compares between the employing group in twos,
P＜0.05 for difference has statistical significance.
3, result
Compare with the blank group, atropine model group gastric emptying rate and intestinal propelling rate reduce,
P＜0.01; Compare with the atropine model group, water extract+atropine group gastric emptying rate and intestinal propelling rate increase,
P＜0.05 Fructus Foeniculi volatile oil+atropine group and mosapride+atropine group compares, gastric emptying rate and intestinal propelling rate increase,
P＜0.01; Compare with water extract+atropine group, Fructus Foeniculi volatile oil+atropine group and mosapride+atropine group gastric emptying rate and intestinal propelling rate increase,
P＜0.01.The results are shown in Table 1.
Table 1: mice gastric emptying rate and the comparison of intestinal propulsion rate (
, n=10)
Annotate: with blank group ratio, *
P＜0.01, #
P＜0.05; With atropine model group ratio, ●
P＜0.01, zero
P＜0.05; With water extract group ratio, ▲
P＜0.01.
, discuss
Fructus Foeniculi nature and flavor suffering, temperature are returned taste Liver and kidney warp, and dispersing cold for relieving pain is arranged, and the regulating qi-flowing for harmonizing stomach effect can be transferred smooth gastrointestinal mechanism of qi, coordinate the taste lifting, reach therapeutic purposes to recover the taste elevating function, are used for colic of cold type stomachache, abdominal distention, lack of appetite and vomiting
[1], be usually used in treating the gastrointestinal motility disorder disease clinically.
Pharmacology of Chinese materia medica
[4]Think that hot flavor Chinese medicine can be regulated the gastrointestinal smooth myokinesis, the effect of its relieving distension by promoting circulation of QI and the adjusting of gastrointestinal smooth muscle have substantial connection.Modern medicine thinks that the gastrointestinal motility disorder disease is relevant with multiple factor, and its main pathophysiological basis is delayed gastric emptying and small intestinal transfer function obstacle.
This experiment gives injecting atropine in the mouse peritoneal with the experimental technique of classics, has blocked the effect of m receptor, gastric emptying rate and intestinal propelling rate all significantly be lower than the blank group (
P＜0.01), illustrate that it is successful that atropine causes mice gastrointestinal motility disorder animal model; Compare with the atropine model group, Fructus Foeniculi volatile oil+atropine group, mosapride+atropine group and water extract+atropine group gastric emptying rate and the increase of intestinal propelling rate (
P＜0.05,
P＜0.01), illustrate that Fructus Foeniculi volatile oil, mosapride and water extract can resist gastrointestinal motility disorder due to the atropine; Compare with water extract+atropine group, Fructus Foeniculi volatile oil+atropine group and mosapride+atropine group gastric emptying rate and the increase of intestinal propelling rate (
P＜0.01), illustrate that Fructus Foeniculi volatile oil and mosapride are better than water extract to gastric emptying and intestinal progradation; Compare with the blank group, Fructus Foeniculi volatile oil+atropine group and mosapride+atropine group gastric emptying rate and intestinal propelling rate no difference of science of statistics, water extract+atropine group gastric emptying rate and the reduction of intestinal propelling rate (
P＜0.05), illustrate Fructus Foeniculi volatile oil and mosapride can fine antagonism atropine due to gastrointestinal motility disorder, water extract can partly resist gastrointestinal motility disorder due to the atropine.
Studies confirm that acetylcholine produces excitable activity at digestive tract, promote gastrointestinal smooth muscle to shrink
[5], and atropine is a kind of antagonist of non-selective M cholinoceptor, can the irritability of gastrointestinal smooth muscle be descended by suppressing the effect of acetylcholine, reduces the amplitude and the frequency of wriggling.So Fructus Foeniculi volatile oil and water extract may be by the antagonism of antagonism atropine to the M cholinoceptor, increase the secretion of gastrointestinal tract excitatory neurotransmitter acetylcholine, thereby promote gastrointestinal motility, exact mechanism still remains further research.
This description of test, Fructus Foeniculi extract has facilitation to gastrointestinal motility disorder, Fructus Foeniculi volatile oil is topmost active component, so when decoction contains Fructus Foeniculi Chinese medicine decoction treatment gastrointestinal motility disorder disease, Fructus Foeniculi should be down back, to reduce the volatilization of main active Fructus Foeniculi volatile oil, increase therapeutic effect.
List of references:
[1] Chinese Pharmacopoeia Commission. Pharmacopoeia of People's Republic of China (an one), Beijing: Chinese Medicine science and technology publishing house, 2010:44-45.
[2] He Jinming, Xiao Yanhui, Wu Jingling. concocting method is to the influence of Fructus Foeniculi quintessence oil extraction ratio and component ratio thereof. the time precious traditional Chinese medical science traditional Chinese medicines, 2008,19(11): 2598-2600.
[3] Shi Xinyou, modern medicine experimental zoology, Beijing: People's Medical Officer Press, 2000:335.
[4] Xu Xiaoyu, pharmacology of Chinese materia medica: the modern times understanding of flavour of a drug, the 1st edition, Beijing: People's Health Publisher, 2005:6.
[5] Zhu Changgeng, Li Yunqing, Gu Xiaosong, neuroanatomy, the 2nd edition, Beijing: People's Health Publisher, 2009:710,218,747.
Four, the anti-inflammatory and analgesic effect of Fructus Foeniculi volatile oil
Purpose: antiinflammatory, the analgesic activity of research Fructus Foeniculi volatile oil provide scientific basis for instructing clinical rational drug use.
Fructus Foeniculi is the dry mature fruit of samphire Fructus Foeniculi, and nature and flavor suffering, temperature are returned taste Liver and kidney warp, have dispersing cold for relieving pain, and the regulating qi-flowing for harmonizing stomach effect is used for colic of cold type stomachache, abdominal distention, lack of appetite and vomiting
[1], its main component is water solublity and volatile oil two parts, wherein the main body composition of volatile oil is trans-anethole, estragole, fenchone and limonene composition
[2]The effective ingredient of its regulating QI to relieve pain is inquired in this experiment in the pharmacological action of antiinflammatory, ease pain by Fructus Foeniculi volatile oil.
The l material
1.1 medicine
The preparation of Fructus Foeniculi (Yumen, Gansu, the place of production) volatile oil: restrain Fructus Foeniculi coarse powder 5 hours with standard water steam distillation unit continuous still 500, reuse normal hexane liquid is used anhydrous Na after extracting 5 times
2SO
4Drying, unnecessary solvent is removed in distillation, obtains 13.5 gram Fructus Foeniculi volatile oils (productive rate is 2.7 %), and with brown bottled, sealing places the preservations of 4 ℃ of refrigerators, and the time spent is mixed with high and low two dosage groups by a certain percentage with gained quintessence oil and soil temperature and distilled water
[3]Water-soluble of aspirin: Astra (Wuxi) pharmaceutical Co. Ltd product, lot number: 2010408; Ovum Gallus domesticus album (get fresh Ovum Gallus domesticus album and be mixed with the solution of concentration 10 %, be standby) with water for injection; Hydrocortisone powder: Shandong Xinhua Pharmaceutical Factory product, lot number: 201003121; Glacial acetic acid: Xi'an chemical reagent factory product, lot number: 20090805; Dimethylbenzene: Xi'an chemical reagent factory product, lot number: 20080712; Tween 80: Dalian medicine grouping of the world economy glass company, lot number: 20071206.
Instrument
Electronic analytical balance: prunus mume (sieb.) sieb.et zucc. Teller one holder benefit instrument (Shanghai) Co., Ltd., model AE240S; 721 type spectrophotometers: Shanghai the 3rd analytical tool factory makes; The standard water steam distillation unit: the general day instrument Manufacturing Co., Ltd in Changzhou makes.
Animal
Cleaning level Kunming mouse, 18-22 g; Cleaning level SD rat, 160～180g; Provide by The Fourth Military Medical University of P.L.A's Experimental Animal Center.
2, method and result
2.1 the effect of xylol induced mice auricular concha inflammation
[4,5]:
40 of healthy Male Kunming strain mice, body weight 18～22g is divided into 4 groups at random by body weight, 10 every group.
(1) blank group: irritate stomach normal saline 20ml/kg; (2) positive controls: irritate stomach hydrocortisone 20mg/kg; (3) the heavy dose of group of volatile oil: irritate stomach Fructus Foeniculi volatile oil 600mg/kg; (4) volatile oil small dose group: irritate stomach Fructus Foeniculi volatile oil 150 mg/kg.Every day 1 time, continuous 3d, behind the last gastric infusion lh, every mouse right ear evenly is coated with dimethylbenzene 0.2 ml and causes inflammation, and left ear does not deal with.Behind the 4h, disconnected marrow is put to death, and cuts ears along the ear baseline, lays circular auricle with 7 mm card punch at bilateral auricular concha same position, weighs.The difference of left and right sides ear weight is an inflammation swelling degree.Inhibitory rate of intumesce=(blank group weight one administration group weight)/blank group weight * 100 %.The results are shown in Table 1.
Table 1 Fructus Foeniculi volatile oil to the influence of mice dimethylbenzene auricular concha inflammation (
, n=10)
Annotate: compare # with the blank group
P＜0.01
2.2Rat paw edema experiment due to the Ovum Gallus domesticus album
[4,5]
Healthy male SD rat, body weight (160 ± 10) g is divided into 5 groups at random, 10 every group.(1) blank group and model control group: irritate stomach normal saline 10 ml/kg; (2) positive controls: irritate stomach hydrocortisone 13 mg/kg; (3) the heavy dose of group of volatile oil: irritate stomach Fructus Foeniculi volatile oil 400 mg/kg; (4) volatile oil small dose group: irritate stomach Fructus Foeniculi volatile oil 100 mg/kg.Every day 1 time, continuous 3d is after the last administration, measure right back sufficient volume with toes volumetric measurement instrument, except that the blank group, all the other respectively organize right back sufficient subcutaneous injection 10% fresh-laid egg clear liquid 0.05 ml, measurement 1,3,5, the right back sufficient volume of 7h rat, causing the volumetrical difference of scorching front and back toes is the swelling degree.The results are shown in Table 2.
Table 2: Fructus Foeniculi volatile oil to the antiinflammatory action of rat Ovum Gallus domesticus album pedal swelling (
, n=10)
Annotate: compare # with the blank group
P＜0.01; Compare with model control group
P＜0.05, ●
P＜0.01.
The effect of the mouse writhing reaction that Dichlorodiphenyl Acetate brings out
[6]
40 of Kunming mouses, male and female half and half, body weight 18～22g is divided into 4 groups at random, 10 every group.(1) blank group: irritate stomach normal saline 20 ml/kg; (2) positive controls: irritate stomach aspirin 200 ㎎/kg; (3) the heavy dose of group of Fructus Foeniculi volatile oil: irritate stomach Fructus Foeniculi volatile oil 600 ㎎/kg; (4) Fructus Foeniculi volatile oil small dose group: irritate stomach Fructus Foeniculi volatile oil 150 ㎎/kg.More than equal administration every day of each treated animal 1 time, 3d continuously, 1h after the last administration, lumbar injection 0.6% acetum 0.2ml/ only writes down mouse writhing number of times in 30 minutes, the results are shown in Table 5.Suppression ratio=(matched group is turned round body number of times average one medication group and turned round body number of times average)/matched group is turned round body number of times average * 100%.The results are shown in Table 3.
Table 3: the influence of the mouse writhing reaction that the Fructus Foeniculi volatile oil Dichlorodiphenyl Acetate brings out (
± s, n=10)
Annotate: compare with the blank group: #
P＜0.01.
Statistical procedures
All experimental datas with means standard deviation (
The one factor analysis of variance in the SPSS12.0 statistical software is adopted in ± s) expression, does not relatively adopt the LSD-t check between on the same group,
P＜0.05 has statistical significance for difference.
3, discuss
Inflammation is that the biological tissue with vascular system is invaded the defense reaction that is taken place to damage or virulence factor, early stage in inflammation, some inflammatory mediator stimulates blood vessel, distend the blood vessels, blood vessel endothelium asks that the crack enlarges, and the blood vessel wall permeability increases, liquid, protein and leukocyte etc. in the blood plasma are exuded to interstice, increase along with oozing out causes swollen tissue, and its typical characteristic is local red, swollen, hot, bitterly and dysfunction; The author adopts mice caused by dimethylbenzene xylene auricle edema, Ovum Gallus domesticus album to cause 2 kinds of classical antiinflammatory animal models of rat paw edema to carry out pharmacodynamic experiment, finds that Fructus Foeniculi volatile oil can be good at Mice Auricle and the rat paw edema that inflammation-inhibiting causes, and has antiinflammatory action.
Pain is the central nervous system with the result behind various information integrated in the body internal and external environment, various neurotransmitters and receptor thereof participate in this process by different way at each position of maincenter, the mouse writhing experiment is the screening model commonly used of research analgesic, this experimental result shows, Fructus Foeniculi volatile oil can alleviate Encelialgia, has analgesic activity.
Pharmacology of Chinese materia medica
[6]Think that mostly be plant volatile oil, glycoside, alkaloid with the closely-related material of suffering flavor effect, hot flavor Chinese medicine has anti-inflammatory, suppresses the outgrowth effect of tissue abnormalities, for the Fructus Foeniculi volatile oil antiinflammatory action provides the pharmacology foundation.The traditional Chinese medical science thinks that " stagnation of QI and blood may bring about pain, general rule are not bitterly ", pharmacology of Chinese materia medica think, hot flavor Chinese medicine has the rheology of the blood flow of improvement, antithrombotic effect, for the Fructus Foeniculi volatile oil analgesic activity provides the pharmacology foundation.Given this, use the decoction contain Fructus Foeniculi treatment stomachache clinically, Fructus Foeniculi should the back down, to reduce the volatilization of volatile oil.
List of references:
[1] Chinese Pharmacopoeia Commission. Pharmacopoeia of People's Republic of China (an one) [M], Beijing: Chinese Medicine science and technology publishing house, 2010:44-45.
[2] Zhong Ruimin, Xiao Zaijun, Zhang Zhen is bright etc., Fructus Foeniculi XIANGZI derived essential oil and antibacterial activity research [J] thereof, chemistry of forest product and industry, 2007,27(6): 36-40.
[3] Qi Chen, herbal pharmacology research methodology [M], the 3rd edition, Beijing: People's Health Publisher, 2006:68-70.
[4] Xu Shuyun, old repairing, pharmacological experimental methodology [M], the 3rd edition, Beijing: People's Health Publisher, 2002:882.
[5] Xu Yanhua, Song Yanchun, Yan Shumei etc., the research of medicinal capsule for treating rheumatalgia anti-inflammatory and analgesic effect [J], Chinese herbal medicine, 2003,34(5): 452-454.
[6] Xu Xiaoyu, pharmacology of Chinese materia medica [M], the 1st edition, Beijing: People's Health Publisher, 2005:5-6.
Claims (2)
1. Fructus Foeniculi volatile oil is used to prepare the application of prevention of postoperative abdominal adhesions medicine.
2. application as claimed in claim 1 is characterized in that, described medicine be external application, oral or the injection various dosage forms.
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| CN102048715A (en) * | 2010-12-21 | 2011-05-11 | 周彦芳 | New effect and applications of trans-acomhole |
| CN102093931A (en) * | 2010-12-09 | 2011-06-15 | 武汉大学 | Method for preparing dill seed essential oil and prevention and control effect thereof on sclerotinia rot of colza |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1372968A (en) * | 2002-03-18 | 2002-10-09 | 范川 | Chinese medicine for treating ureteral occlusion |
| CN1422648A (en) * | 2002-03-18 | 2003-06-11 | 川北医学院 | Thermal therapeutical medicated bag for recovering intestines and stomach function after abdomen operation and production method |
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| CN1372968A (en) * | 2002-03-18 | 2002-10-09 | 范川 | Chinese medicine for treating ureteral occlusion |
| CN1422648A (en) * | 2002-03-18 | 2003-06-11 | 川北医学院 | Thermal therapeutical medicated bag for recovering intestines and stomach function after abdomen operation and production method |
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| 《中国中西医结合消化杂志》 20061031 方新社 食盐加小茴香治疗肠梗阻62例 339-340 1-2 第14卷, 第5期 2 * |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102093931A (en) * | 2010-12-09 | 2011-06-15 | 武汉大学 | Method for preparing dill seed essential oil and prevention and control effect thereof on sclerotinia rot of colza |
| CN102093931B (en) * | 2010-12-09 | 2012-09-05 | 武汉大学 | Method for preparing dill seed essential oil and prevention and control effect thereof on sclerotinia rot of colza |
| CN102048715A (en) * | 2010-12-21 | 2011-05-11 | 周彦芳 | New effect and applications of trans-acomhole |
| CN105267262A (en) * | 2014-06-10 | 2016-01-27 | 四川江茂医药发展有限公司 | Food, medicine or health care product composition composed of common fennel volatile oil |
| CN104739905A (en) * | 2015-02-15 | 2015-07-01 | 四川大学华西第二医院 | Fennel oral preparation as well as preparation method and new application thereof |
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