CN102008088B - Egg membrane hydrolyzate of cuttlefish egg - Google Patents
Egg membrane hydrolyzate of cuttlefish egg Download PDFInfo
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- CN102008088B CN102008088B CN2010105192599A CN201010519259A CN102008088B CN 102008088 B CN102008088 B CN 102008088B CN 2010105192599 A CN2010105192599 A CN 2010105192599A CN 201010519259 A CN201010519259 A CN 201010519259A CN 102008088 B CN102008088 B CN 102008088B
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- cuttlefish
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Abstract
The invention aims to provide an egg membrane hydrolyzate of a cuttlefish egg for disaggregating an egg membrane structure on the outer layer of the cuttlefish egg; the reagent can be used for processing cuttlefish egg caviar; and the reagent can further be used for observing cuttlefish egg embryo; in the egg membrane hydrolyzate of cuttlefish egg, the egg membrane hydrolyzate of cuttlefish egg per liter comprises the following components according to parts by weight: 17 to 30 parts of NaCl, 0.1 to 0.3 parts of KCl, 1.4 to 1.7 parts of Na2HPO4 and 0.2 to 0.25 parts of KH2PO4, and less than 0.5 parts of enzymic preparation; and the enzymic preparation can disaggregate the outer membrane of cuttlefish egg. The enzymic preparation comprises trypsin; and the enzymic preparation is 50 to 500 U.
Description
Technical field
The present invention relates to siphonopods egg membrane hydrolyzate, particularly egg membrane hydrolyzate of cuttlefish egg.
Background technology
Caviar adopts huso sturgeon ovum or sturgeon ovum more, and general adult fish needs 7 to 12 years ability ovum caviars processed.Therefore, caused its expensive present situation.Excellent caviar full grains are round and smooth, and transparent color and luster is limpid.The outward appearance of caviar is most important for quality, senior caviar, and particle is mellow and full full, and color and luster is limpid transparent, even slightly general golden yellow gloss, so people also have been accustomed to the caviar metaphor is made " gold of black ".Caviar contains the required trace element of skin, mineral salt, protein, amino acid and the basic aliphatic acid of restructuring, has certain health-care efficacy.Sepiella maindroni once was one of the four large fishery in China fishing ground, and was stronger to the adaptability of temperature, distribute at China coast very wide, and be the very high traditional fishery kind of a kind of economic worth.Because overfishing and ecological degeneration since 20 century 70s, decay successively occurred.Last century, the eighties had report Li Xing a word used in person's names etc. to the Primary Study of Sepiella maindroni resource and propagation, and Oceanography Institute Of Zhejiang has broken through the artificial breeding technology of Sepiella maindroni in recent years, and had carried out the stock enhancement experiment work of releasing from 2006 years.Along with the success of propagating artificially, the comprehensive utilization of cuttlefish is also become gradually the focus of concern.Prior art such as the patent No. are 00515997.1 1 kinds of method and apparatus that prepare similar black (granular) caviar of alimentary soft granular caviar, comprise that preparation contains the mixture of collagen, water and edible gelatin in order to form particle, particle is exposed in tea, pigment, salting liquid and the taste component caviar of obtaining with machine separation, flushing, the technique such as painted.Prior art adopts other raw materials to replace, and has reduced cost, can alleviate to a certain extent the pressure of environment, but adopts alternative materials to be difficult to reach consumer's requirement psychologically.Siphonopods, particularly cuttlefish, it has between outer egg membrane and the ovum grain by the special connection bunchiness of outer egg membrane, adopts the common mode of rubbing to add and is difficult for man-hour separating, and efficient hand-manipulated is lower.
Summary of the invention
The purpose of this invention is to provide a kind of egg membrane hydrolyzate of cuttlefish egg, realize dissociating of the outer egg membrane structure of sea grape with this, and can stablize the pH value, improve the efficient of enzyme.This reagent processed can be used for the processing of sea grape caviar, also can be used for sea grape embryo's experiment is observed.
The present invention is achieved in that egg membrane hydrolyzate of cuttlefish egg, and wherein: the egg membrane hydrolyzate of cuttlefish egg of every 1L contains 17 parts to 35 parts NaCl, 0.1 part to 0.3 part KCl, 1.4 parts to 1.7 parts Na by weight
2HPO
4KH with 0.2 part to 0.25 part
2PO
4, less than 0.5 part enzyme preparation; The outer membrane of enzyme preparation energy enzymolysis sea grape.
In order further to optimize technique scheme, the Optimized Measures of taking also comprises: enzyme preparation contains trypsase; Enzyme preparation is 50U to 500U.
Adopt mentioned reagent can remove the outer egg membrane of sea grape, be used for producing the caviar of sea grape, it comprises the steps:
1. oolysis is from step: select complete fresh raw material string shape sea grape, put into egg membrane hydrolyzate of cuttlefish egg and soaked 20 minutes to 8 hours; The sea grape string has outer egg membrane parcel, and toughness is higher, is unsuitable for directly ediblely, therefore adopts egg membrane hydrolyzate of cuttlefish egg to the institutional framework of the outer egg membrane destruction of further dissociating, thereby improves its edibility.
2. select the ovum step: put into preliminary treatment salt solution and soaked 2 hours to 5 hours; Then stir, sieve and choose the ovum grain, the salt solution on ovum grain surface is removed in then rinsing, drains; Be different from the preparation method of common caviar, the present invention adopts the salt solution a little more than former growth waters salinity that ovum is carried out preliminary treatment.The ovum grain of sea grape own is larger, and water content is high, if be put in easily cause in the fresh water suction and the egg membrane that rises brokenly then easily causes the dehydration of ovum grain to be subsided with supersaline water wrinkling, affect outward appearance.
3. salting step: will 2. process the sea grape grain that obtains through step and put into container, the edible salt that adds sea grape grain weight 2% to 7% stirred 2 minutes to 20 minutes, then elimination salt accumulated water.By soaking salt solution, can slough the part moisture in the sea grape, not so long-term storage can cause the fragmentation of egg membrane, and soaking the salt solution time should not be long, the outward appearance that prevents from dewatering atrophy and affect finished product, salt solution also has certain bactericidal action, and can prevent aqtocytolysis.
Step 2. preliminary treatment salt solution is liquid or mixture of ice and water, and temperature is lower than 4 ℃.
The step 2. salinity of preliminary treatment salt solution is 17 ‰ to 40 ‰, and the salinity of preliminary treatment salt solution is higher than waters, the place of production salinity at least 3 ‰ of raw material sea grape string.
Should configure according to the salt content in the waters of choosing sea grape corresponding egg membrane hydrolyzate of cuttlefish egg, follow principle and be so that the NaCl content of egg membrane hydrolyzate of cuttlefish egg more than or equal to the salt content in waters, the raw material sea grape string place of production, can not cause sea grape internal layer and outer egg membrane together to break reducing quality because sea grape absorbs water in egg membrane hydrolyzate of cuttlefish egg.
Enzyme preparation is greater than 50U; Enzyme preparation contains trypsase.The step 1. pH value of egg membrane hydrolyzate of cuttlefish egg is 7.2 to 8.1.The NaCl concentration of egg membrane hydrolyzate of cuttlefish egg is less than or equal to the NaCl concentration of preliminary treatment salt solution.
Compared with prior art, the invention has the advantages that: the present invention adopts Na
2HPO
4And KH
2PO
4And the reagent system of said ratio, the pH value of whole liquid environment can be maintained in the comparatively stable value scope.Adopt the present invention can with the sea grape oolysis from and remove the outer membrane of sea grape and the connection between the outer membrane so that independent between the ovum grain; Reagent system of the present invention is applicable to the large ovum grain of sea grape, the characteristics that moisture is large, can prevent from adding cause behind the ovum grain pH extremely acute variation and affect hydrolysis result; The ovum grain smooth surface that adopts the present invention to process.
The specific embodiment
Below in conjunction with attached embodiment the present invention is described in further detail.
Embodiment: egg membrane hydrolyzate of cuttlefish egg, wherein: the every 1L of egg membrane hydrolyzate of cuttlefish egg contains the KCl of NaCl, the 0.1g of 20g, the Na of 1.48g
2HPO
4KH with 0.2g
2PO
4With the enzyme preparation less than 0.5 part; The outer membrane of enzyme preparation energy enzymolysis sea grape.Enzyme preparation contains trypsase; Enzyme preparation is 100U.
Adopt mentioned reagent to make the method for sea grape caviar: 1. oolysis is from step: select complete fresh raw material string shape sea grape, put into egg membrane hydrolyzate of cuttlefish egg and soaked preferred 40 minutes of the present embodiment 20 minutes to 8 hours; Egg membrane hydrolyzate of cuttlefish egg contains the tryptic enzyme preparation in the 100U/L left and right sides, thereby egg membrane hydrolyzate of cuttlefish egg can dissolve the outer egg membrane of sea grape;
2. select the ovum step: put into preliminary treatment salt solution and soaked 2 hours to 5 hours, the present embodiment was selected 2 hours; Then stir, sieve and choose the ovum grain, the salt solution on ovum grain surface is removed in then rinsing, drains; Be different from the preparation method of common caviar, the present invention adopts the salt solution a little more than former growth waters salinity that ovum is carried out preliminary treatment.The ovum grain of sea grape own is larger, and water content is high, if be put in easily cause in the fresh water suction and the egg membrane that rises brokenly then easily causes the dehydration of ovum grain to be subsided with supersaline water wrinkling, affect outward appearance.
3. salting step: will 2. process the sea grape grain that obtains through step and put into container, the edible salt that adds sea grape grain weight 3% stirred 2 minutes to 20 minutes, and the present embodiment adopts 8 minutes mixing time, then elimination salt accumulated water.By soaking salt solution, can slough the part moisture in the sea grape, not so long-term storage can cause the fragmentation of egg membrane, and soaking the salt solution time should not be long, the outward appearance that prevents from dewatering atrophy and affect finished product, salt solution also has certain bactericidal action, and can prevent aqtocytolysis.
Step 2. preliminary treatment salt solution is liquid or mixture of ice and water, and temperature is lower than 4 ℃, and the present embodiment is selected 0 ℃ to 3 ℃, and the side has ice cube to control temperature in lower level in the preliminary treatment salt solution.
The step 2. salinity of preliminary treatment salt solution is 20 ‰, and this salinity is a little more than the used aquaculture water salinity of the present embodiment sea grape string.The aquaculture water salinity of the present embodiment is 15 ‰ to 16 ‰.
Should configure according to the salt content in the waters of choosing sea grape corresponding egg membrane hydrolyzate of cuttlefish egg, follow principle and be so that the NaCl content of egg membrane hydrolyzate of cuttlefish egg more than or equal to the salt content in waters, the raw material sea grape string place of production, can not cause sea grape internal layer and outer egg membrane together to break reducing quality because sea grape absorbs water in egg membrane hydrolyzate of cuttlefish egg.
Step is the pH value meta-alkalescence of egg membrane hydrolyzate of cuttlefish egg 1., can improve the efficient of enzyme preparation in the egg membrane hydrolyzate of cuttlefish egg, can regulate as required Na
2HPO
4And KH
2PO
4Ratio improve pH.Improve Na
2HPO
4Ratio the pH of egg membrane hydrolyzate of cuttlefish egg is further raise, when too high, can add an amount of HCl and turn down pH.
Although described the present invention in conjunction with preferred embodiment; so it is not to limit the present invention; any those skilled in the art; in the situation that do not break away from the spirit and scope of the present invention; can implement to the theme of listing displacement and the modification of various changes, coordinate here, so protection scope of the present invention is as the criterion when looking the claim restricted portion that proposes.
Claims (1)
1. egg membrane hydrolyzate of cuttlefish egg, it is characterized in that: the egg membrane hydrolyzate of cuttlefish egg of every 1L contains 17 parts to 35 parts NaCl, 0.1 part to 0.3 part KCl, 1.4 parts to 1.7 parts Na by weight
2HPO
4KH with 0.2 part to 0.25 part
2PO
4, less than 0.5 part enzyme preparation; The outer membrane of described enzyme preparation energy enzymolysis sea grape; The NaCl content of described egg membrane hydrolyzate of cuttlefish egg is more than or equal to the salt content in waters, the raw material sea grape string place of production; Described enzyme preparation contains trypsase; Described enzyme preparation is 50U to 500U.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105192599A CN102008088B (en) | 2010-10-22 | 2010-10-22 | Egg membrane hydrolyzate of cuttlefish egg |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105192599A CN102008088B (en) | 2010-10-22 | 2010-10-22 | Egg membrane hydrolyzate of cuttlefish egg |
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| Publication Number | Publication Date |
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| CN102008088A CN102008088A (en) | 2011-04-13 |
| CN102008088B true CN102008088B (en) | 2013-04-17 |
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| CN104522814B (en) * | 2015-01-05 | 2017-05-03 | 宁德市南海水产科技有限公司 | Processing technology of sepiella maindroni egg beverage |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101368179A (en) * | 2008-05-31 | 2009-02-18 | 安徽师范大学 | Reagent kit for extracting DNA in Chinese alligator chorion film and use method thereof |
| CN101493414A (en) * | 2009-02-19 | 2009-07-29 | 浙江海洋学院 | Fluorescent dyeing method for cuttlefish embryonic development and nuclear action observation |
| CN101647419A (en) * | 2009-09-18 | 2010-02-17 | 湖北省水产科学研究所 | Separation method of fries and roes after hatching of loach fertilized roes |
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2010
- 2010-10-22 CN CN2010105192599A patent/CN102008088B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101368179A (en) * | 2008-05-31 | 2009-02-18 | 安徽师范大学 | Reagent kit for extracting DNA in Chinese alligator chorion film and use method thereof |
| CN101493414A (en) * | 2009-02-19 | 2009-07-29 | 浙江海洋学院 | Fluorescent dyeing method for cuttlefish embryonic development and nuclear action observation |
| CN101647419A (en) * | 2009-09-18 | 2010-02-17 | 湖北省水产科学研究所 | Separation method of fries and roes after hatching of loach fertilized roes |
Non-Patent Citations (3)
| Title |
|---|
| 刘万顺等.海洋无脊椎动物消化酶的研究 I、紫贻贝、日本蟳、滨螺消化酶的初步分析及其应用.《山东海洋学院学报》.1988,第18卷(第1期), * |
| 刘振勇等.曼氏无针乌贼胚胎发育的初步观察.《渔业科学进展》.2009,第30卷(第5期), * |
| 易詠兰等.鱼类囊胚细胞和卵的电融合.《水生生物学报》.1988,第12卷(第2期), * |
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