CN102002113A - Method for preparing tea plant mushroom fruit body polysaccharide - Google Patents
Method for preparing tea plant mushroom fruit body polysaccharide Download PDFInfo
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- CN102002113A CN102002113A CN2010105782030A CN201010578203A CN102002113A CN 102002113 A CN102002113 A CN 102002113A CN 2010105782030 A CN2010105782030 A CN 2010105782030A CN 201010578203 A CN201010578203 A CN 201010578203A CN 102002113 A CN102002113 A CN 102002113A
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Abstract
The invention belongs to the technical field of bioengineering, and in particular relates to a method for preparing tea plant mushroom fruit body polysaccharide. The tea plant mushroom fruit body polysaccharide is white or light yellow powder and is prepared from the tea plant mushroom fruit body by the following steps of: crushing; degreasing; performing hot water extraction; centrifuging; concentrating supernatant; precipitating with ethanol; deproteinizing; decoloring; dialyzing; and freeze-drying, wherein 3.03g of the dried fruit body polysaccharide is extracted from every 100g of fruit body; and the measured polysaccharide content is 67.3 percent. The method can be applied to the preparation of the health-care products with functions of enhancing immunity and resisting fatigue.
Description
Technical field
The present invention relates to a kind of preparation method of tea tree mushroom fruitbody polysaccharide.Belong to technical field of bioengineering.
Background technology
Tea tree mushroom belongs to Basidiomycetes, excrement rust umbrella section, and the field mushroom belongs to, and has another name called tea mushroom, oil tea mushroom, refreshing mushroom.Be a kind of edible mushrooms new variety that development in recent years is got up.Tea tree mushroom is nutritious, and the protein content height contains the amino acid of needed by human, and mineral elements such as abundant vitamin B group and potassium, sodium, calcium, magnesium, iron, zinc are arranged.The traditional Chinese medical science thinks to have higher pharmaceutical use by flat, the Gan Wen, nontoxic of this mushroom property, heat-clearing, flat liver are arranged, makes eye bright, the effect of invigorating the spleen.Its extract polysaccharide up to 80-90%, is a kind of green food that integrates nutrition, health care, physiotherapy to the inhibiting rate of small white mouse sarcoma S-180 and ehrlich carcinoma.
Summary of the invention
The preparation method who the purpose of this invention is to provide a kind of tea tree mushroom fruitbody polysaccharide.
Technical scheme of the present invention is: the tea tree mushroom sporophore passed through to pulverize, and degreasing, the hot water lixiviate, centrifugal, supernatant concentration, ethanol sedimentation, deproteinated, decolouring, the dialysis postlyophilization obtains.
The tea tree mushroom fruitbody polysaccharide preparation method of the present invention's invention, extracting yield is 3.03g/100g sporophore dry weight, recording polysaccharide content is 67.3%.Confirm through preliminary research, nontoxic substantially, can be used as raw material and be applied to fields such as protective foods.
Specifically, at first, we pulverize commercially available exsiccant tea tree mushroom sporophore with pulverizer, carry out degreasing with the organic solvent ethyl acetate then, 80 ℃ of-90 ℃ of hot water lixiviates, amount of water is 20 times of volume degreasing after products, total extraction time is 12h, extract 2 times, extracting solution concentrates back 4 times of volume dehydrated alcohols of adding and analyses centrifugal collecting precipitation at 4 ℃ of alcohol, ethanol, redissolve with distilled water ether washing back, and the Sevage method is removed floating preteins, H
2O
2Decolouring, distill water dialysis obtain the tea tree mushroom fruitbody polysaccharide after the lyophilize.
Embodiment
Following example will specify working method of the present invention, but can not be as limitation of the invention.
Example one
Tea tree mushroom sporophore after 100g pulverizes adds the Soxhelt extractor, with ethyl acetate as solvent, degreasing 6h refluxes, collect solid substance hot water lixiviate, extract 80 ℃-90 ℃ of temperature, amount of water is 20 times of volume solid substances, total extraction time is 10h, extract 3 times, 3 times of volume 95% ethanol of adding were analysed at 4 ℃ of alcohol and are spent the night after extracting solution concentrated, and the centrifugal throw out that gets of 8000rpm, throw out are pressed the volume adding chloroform of polysaccharide soln 1/5 with distilled water redissolution back, the propyl carbinol that adds chloroform volume 1/5 then mixes back vibration 20min.Protein and chloroform-propyl carbinol generate gel, and the centrifugal 20min of 4000rpm collects supernatant liquor, repeatable operation 8 times to chloroform-n-butanol layer not muddy till.In supernatant liquor, add 95% ethanol then and put 4 ℃ of refrigerator overnight, next day centrifugal collecting precipitation.Throw out is transferred pH to 9.0 with 5% polysaccharide soln with NaOH after redissolving with distilled water, drips 20% H then
2O
2(consumption be polysaccharide soln volume 15%) keeps PH8.0-9.0, and termination reaction behind the 4h adds 95% ethanol and puts 4 ℃ of refrigerator overnight, next day centrifugal collecting precipitation.Get 50mL after throw out redissolves with distilled water and pack in the dialysis tubing, tighten sack, be suspended in the distilled water, dialysis 48h middlely changed water once every 12 hours.Obtain tea tree mushroom fruitbody polysaccharide 3.03g after the lyophilize, recording polysaccharide content with the phenolsulfuric acid method is 67.3%.
Claims (7)
1. the extracting method of a tea tree mushroom sporozoite entity polysaccharide is characterized in that outward appearance is white or pale yellow powder, is that the tea tree mushroom sporophore is passed through to pulverize, degreasing, and the hot water lixiviate, centrifugal, supernatant concentration, ethanol sedimentation, Sevage method deproteinated, H
2O
2Decolouring, the dialysis postlyophilization obtains.Extracting yield is 3.03g/100g sporophore dry weight, and recording polysaccharide content is 67.3%.Can be applicable to preparation and have strengthening immunity, the healthcare products of anti-fatigue effect.
2. the preparation method of tea tree mushroom fruitbody polysaccharide as claimed in claim 1 is characterized in that described degreasing is the tea tree mushroom sporophore to be pulverized the back add the Soxhelt extractor, and as solvent, degreasing 6h refluxes with ethyl acetate.
3. the preparation method of tea tree mushroom fruitbody polysaccharide as claimed in claim 1 is characterized in that 80 ℃-90 ℃ of described hot water extraction temperatures, and amount of water is 20 times of volume solid substances, and total extraction time is 10h, extracts 3 times.
4. the preparation method of tea tree mushroom fruitbody polysaccharide as claimed in claim 1 is characterized in that described ethanol sedimentation is meant that hot water extraction liquid concentrates the back and adds 3 times of volume 95% ethanol and analyse at 4 ℃ of alcohol and spend the night, 8000rpm centrifugal throw out.
5. the preparation method of tea tree mushroom fruitbody polysaccharide as claimed in claim 1 is characterized in that described Sevage method removal floating preteins is the volume adding chloroform by polysaccharide soln 1/5, adds the propyl carbinol of chloroform volume 1/5 then, mixes back vibration 20mi; Protein and chloroform-propyl carbinol generate gel, and the centrifugal 20min of 4000rpm collects supernatant liquor, repeatable operation 8 times to chloroform-n-butanol layer not muddy till.
6. the preparation method of tea tree mushroom fruitbody polysaccharide as claimed in claim 1 is characterized in that described H
2O
2Decolouring is that 5% polysaccharide soln is transferred pH to 9.0 with NaOH, drips 20% H then
2O
2(consumption be polysaccharide soln volume 15%) keeps PH8.0-9.0, and termination reaction behind the 4h adds 95% ethanol and puts 4 ℃ of refrigerator overnight, next day centrifugal collecting precipitation.
7. the preparation method of tea tree mushroom fruitbody polysaccharide as claimed in claim 1, it is characterized in that described dialysis is meant that 4 ℃ of alcohol are analysed and gets 50mL after centrifugal sediment after spending the night redissolves with distilled water and pack in the dialysis tubing, tighten sack, be suspended in the distilled water, dialysis 48h, water is changed once every 12h in the centre.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105782030A CN102002113A (en) | 2010-12-08 | 2010-12-08 | Method for preparing tea plant mushroom fruit body polysaccharide |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2010105782030A CN102002113A (en) | 2010-12-08 | 2010-12-08 | Method for preparing tea plant mushroom fruit body polysaccharide |
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| CN102002113A true CN102002113A (en) | 2011-04-06 |
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| CN2010105782030A Pending CN102002113A (en) | 2010-12-08 | 2010-12-08 | Method for preparing tea plant mushroom fruit body polysaccharide |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102276744A (en) * | 2011-06-17 | 2011-12-14 | 福建农林大学 | Agrocybe cylindracea active polysaccharide and preparation method thereof |
| CN102429855A (en) * | 2011-12-15 | 2012-05-02 | 江南大学 | Preparation method of agrocybe cylindracea fruit body extracting solution with whitening efficacy |
| CN102532341A (en) * | 2012-02-17 | 2012-07-04 | 河北师范大学 | Extraction method and application of chroogomphus rutilus polysaccharide |
| CN102763797A (en) * | 2012-08-03 | 2012-11-07 | 天津红日药业股份有限公司 | Complex polysaccharide health caring and treating formula and preparing method thereof |
| CN104017098A (en) * | 2014-05-28 | 2014-09-03 | 郑州轻工业学院 | Method for directly extracting lentinus-edodes active polysaccharide from waste lentinus-edodes sticks |
| CN104262505A (en) * | 2014-10-29 | 2015-01-07 | 安徽省科普产品工程研究中心有限责任公司 | Method for extracting edible mushroom polysaccharide |
| CN104844721A (en) * | 2015-04-20 | 2015-08-19 | 苏州经贸职业技术学院 | Extraction and separation method of Agrocybe aegirit polysaccharides |
| WO2019218507A1 (en) * | 2018-05-14 | 2019-11-21 | 广东省微生物研究所（广东省微生物分析检测中心） | Preparation method for agrocybe cylindracea extract and application thereof in preparation of uric acid lowering drug |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102276744A (en) * | 2011-06-17 | 2011-12-14 | 福建农林大学 | Agrocybe cylindracea active polysaccharide and preparation method thereof |
| CN102276744B (en) * | 2011-06-17 | 2013-06-05 | 福建农林大学 | Agrocybe cylindracea active polysaccharide and preparation method thereof |
| CN102429855A (en) * | 2011-12-15 | 2012-05-02 | 江南大学 | Preparation method of agrocybe cylindracea fruit body extracting solution with whitening efficacy |
| CN102532341A (en) * | 2012-02-17 | 2012-07-04 | 河北师范大学 | Extraction method and application of chroogomphus rutilus polysaccharide |
| CN102763797A (en) * | 2012-08-03 | 2012-11-07 | 天津红日药业股份有限公司 | Complex polysaccharide health caring and treating formula and preparing method thereof |
| CN104017098A (en) * | 2014-05-28 | 2014-09-03 | 郑州轻工业学院 | Method for directly extracting lentinus-edodes active polysaccharide from waste lentinus-edodes sticks |
| CN104262505A (en) * | 2014-10-29 | 2015-01-07 | 安徽省科普产品工程研究中心有限责任公司 | Method for extracting edible mushroom polysaccharide |
| CN104844721A (en) * | 2015-04-20 | 2015-08-19 | 苏州经贸职业技术学院 | Extraction and separation method of Agrocybe aegirit polysaccharides |
| CN104844721B (en) * | 2015-04-20 | 2017-01-25 | 苏州经贸职业技术学院 | Extraction and separation method of Agrocybe aegirit polysaccharides |
| WO2019218507A1 (en) * | 2018-05-14 | 2019-11-21 | 广东省微生物研究所（广东省微生物分析检测中心） | Preparation method for agrocybe cylindracea extract and application thereof in preparation of uric acid lowering drug |
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Application publication date: 20110406 |