CN101555435B - Method for extracting antrodia cinnamomea oil from antrodia cinnamomea fruiting bodies - Google Patents
Method for extracting antrodia cinnamomea oil from antrodia cinnamomea fruiting bodies Download PDFInfo
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- CN101555435B CN101555435B CN2009101069681A CN200910106968A CN101555435B CN 101555435 B CN101555435 B CN 101555435B CN 2009101069681 A CN2009101069681 A CN 2009101069681A CN 200910106968 A CN200910106968 A CN 200910106968A CN 101555435 B CN101555435 B CN 101555435B
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Abstract
The invention relates to a method for extracting active materials, in particular to a method for extracting antrodia cinnamomea oil from antrodia cinnamomea fruiting bodies. The method comprises the following steps of: 1) crushing the antrodia cinnamomea fruiting bodies into antrodia cinnamomea fruiting body powder; 2) loading the crushed antrodia cinnamomea fruiting body powder into an extractionkettle of a supercritical CO2 extraction device, and adding ethanol-water mixed liquor as an entrainer; 3) after extraction, putting the crushed antrodia cinnamomea fruiting body powder into a separation kettle for separation; 4) separating the mixed liquor of the separated antrodia cinnamomea oil and the entrainer by a high-speed centrifugal device with the rotating speed being 6000-49000 circles to obtain the antrodia cinnamomea oil; and 5) collecting and weighing the separated extract, namely the antrodia cinnamomea oil. The invention has the beneficial effects that the method can be used for replacing the method for extracting the antrodia cinnamomea oil from antrodia cinnamomea spores, and the yield of extraction can be as high as 40%; the method can be used for industrialized mass production, with relatively low cost, relatively high oil yield, and high absorptivity in body after taking; and the content of effective ingredients is higher than that in the antrodia cinnamomea spores, thus providing consumers with new products and improving the added value of products.
Description
Technical field
The present invention relates to a kind of method that extracts active substance, refer more particularly to a kind of method for extracting antrodia cinnamomea oil from antrodia cinnamomea fruiting bodies.
Background technology
The camphor tree sesame is the exclusive kind in tw Taiwan, and it is most precious medicinal material that the aboriginal of Taiwan Province all is considered as the camphor tree sesame, is " panacea " of treatment severe, liver cirrhosis.The whole world only is grown in the mountain area, Taiwan, and is between 450 to 2000 meters of height above sea level, century-old above and classify as on the Cinnamomum kanahirai hay tree of national treasure level child care class.The camphor tree sesame claims Antrodia camphorata, Cinnamomum kanahirai hay mushroom again, belongs to Aphyllophorales, perennial gill fungus mushroom, surperficial redness as smart as a new pin, in spore is arranged, its sporophore does not have stem, and intensive camphor tree fragrance is arranged, flavor is hot, bitter.The camphor tree sesame mainly is distributed in the ground such as peach garden, Miaoshu, southern throwing, Tai Dong and Kaohsiung of China Taiwan at present, and all the other regions are then very rare.The hollow director of camphor tree sesame wall within Cinnamomum kanahirai hay tree is rotten goes out, or the wet surface of Cinnamomum kanahirai hay shade undercurrent that withered volt falls grows, and is not easy to be found, and therefore is of great rarity.The camphor tree sesame has the yellow camphor tree fragrance of intensive, and outward appearance becomes tabular or stalactitic, back side chocolate, the outside of belly is beautiful orange, is poroid, mycelia is elongated, have every, in a large amount of conidiums are arranged, conidium is orange, digested tankage look or yellow because of the culture condition difference.
In recent years, after the message that the camphor tree sesame has a magical curative effect spread out of, also when the river rises the boat goes up day by day for the price of natural wild camphor tree sesame, the most expensive wild fungus in the whole world of being known as at present, and market price does not wait up to more than 10 ten thousand yuan of every jin New Taiwan Currencies to hundreds thousand of units.Be worth and place oneself above out and away already on traditional high precious medicinal materials such as glossy ganoderma, genseng, Cordyceps sinensis, the king among the king in the medicinal material of therefore can saying so.
Contain multiple physiologically active ingredient in the camphor tree sesame, as polysaccharide, triterpene compound, sudismase (SOD) adenosine, protein (containing immune protein), multivitamin, trace element (calcium, phosphorus, paper mulberry), nucleic acid, lectin, amino acid, cholesterol, xylogen, blood pressure stabilization material etc.That it physiological active functions that possesses has is antitumor, strengthening immunity, antiviral, antianaphylaxis, hypertension, inhibition platelet aggregation, hypotensive, decreasing cholesterol, antibacterium, protection liver etc.
Based on the development of above-mentioned camphor tree sesame, now be badly in need of a kind of new extraction camphor tree sesame oil method, satisfy the continual renovation development of the various products of camphor tree sesame.
Summary of the invention
The present invention is directed to above-mentioned the deficiencies in the prior art technical problem to be solved is: a kind of method with extracting antrodia cinnamomea oil from antrodia cinnamomea fruiting bodies is provided.
The technical solution adopted for the present invention to solve the technical problems is:
A kind of method for extracting antrodia cinnamomea oil from antrodia cinnamomea fruiting bodies, this method may further comprise the steps:
1) earlier camphor tree sesame fruit body powder is broken to 81～499 purpose camphor tree sesame fruit body powders;
2) supercritical CO of packing into of the camphor tree sesame fruit body powder after will pulverizing
2In the extraction kettle of extraction plant, add the alcohol-water mixed solution as entrainment agent, the extracting pressure of extraction kettle is 11MPa～36Mpa; Extraction temperature is 29 ℃～64 ℃; CO
2Fluid flow rate is 35～115kg/h; The extraction time is 0.5h～48h;
3), enter separating still and separate after the extraction, the internal pressure of separation reactor I is 6MP～26MP, and temperature is 31 ℃～65 ℃; Separation reactor I I internal pressure is 4MP～15MP, and temperature is 20 ℃～50 ℃, obtains camphor tree sesame oil and entrainment agent mixed solution;
4) oil of the camphor tree sesame after will separating and entrainment agent mixed solution are that 6000～49000 centrifugal devices that change separate through rotating speed, obtain camphor tree sesame oil;
5) the camphor tree sesame oil after will separating is collected and is weighed.
The proportional range of alcohol-water is in the described entrainment agent: 9.99: 0.01～1: 9.
The weight of described entrainment agent is 1%～39% of camphor tree sesame fruit body powder weight.
The weight of described entrainment agent is 0.1%～9.99% of camphor tree sesame fruit body powder weight.
The weight of described entrainment agent is 20.1%～40% of camphor tree sesame fruit body powder weight.
The yield of described camphor tree sesame sporophore extract camphor tree sesame oil is 20%～40%.
Described high speed centrifugation device rotary speed is 40001～49000 commentaries on classics.
The extracting pressure of described extraction kettle is 11MPa～15Mpa, and extraction temperature is 30 ℃～35 ℃.
Described CO
2Fluid flow rate is 35～60kg/h.
The described extraction time is 3h～48h.
The beneficial effect of a kind of method for extracting antrodia cinnamomea oil from antrodia cinnamomea fruiting bodies of the present invention is:
A kind of method for extracting antrodia cinnamomea oil from antrodia cinnamomea fruiting bodies is provided, be used for replacing with camphor tree Ganoderma lucidum spore extraction camphor tree sesame oil, and the oil yield of extraction can be up to 40%, can be used for industrial mass production, the relative camphor tree Ganoderma lucidum spore extraction of cost is low, its oil yield wants high with respect to the camphor tree Ganoderma lucidum spore, take the intravital specific absorption in back than camphor tree Ganoderma lucidum spore height, the content of effective ingredient is than camphor tree Ganoderma lucidum spore height, for the exploitation of camphor tree sesame provides new channel and thinking, for the human consumer provides new product, improved value-added content of product.
Embodiment
The present invention implements like this:
Embodiment one:
Method for extracting antrodia cinnamomea oil from antrodia cinnamomea fruiting bodies may further comprise the steps:
1) earlier camphor tree sesame fruit body powder is broken to 81 purpose camphor tree sesame fruit body powders;
2) supercritical CO of packing into of the camphor tree sesame fruit body powder after will pulverizing
2In the extraction kettle of extraction plant, and add the alcohol-water mixed solution as entrainment agent, the ratio of alcohol-water is: 9.99: 0.01, the weight of alcohol-water mixed solution was 1% of camphor tree sesame fruit body powder weight, and the extracting pressure of extraction kettle is 11MPa; Extraction temperature is 29 ℃; CO
2Fluid flow rate is 35kg/h; The extraction time is 1h;
3), enter separating still and separate after the extraction, the internal pressure of separation reactor I is 6MP, and working temperature is 31 ℃; Separation reactor I I internal pressure is 4MP, and temperature is 20 ℃;
4) oil of the camphor tree sesame after will separating and entrainment agent mixed solution are that the 6000 high speed centrifugation devices that change separate through rotating speed, obtain camphor tree sesame oil;
5) the camphor tree sesame oil after will separating is collected and is weighed.
Camphor tree sesame sporophore extract camphor tree sesame oil yield is 28% in the present embodiment.
Embodiment two:
Method for extracting antrodia cinnamomea oil from antrodia cinnamomea fruiting bodies may further comprise the steps:
1) earlier camphor tree sesame fruit body powder is broken to 140 purpose camphor tree sesame fruit body powders;
2) supercritical CO of packing into of the camphor tree sesame fruit body powder after will pulverizing
2In the extraction kettle of extraction plant, and add the alcohol-water mixed solution as entrainment agent, the ratio of alcohol-water is: ethanol: water=2: 1, the weight of alcohol-water mixed solution are 4% of camphor tree sesame fruit body powder weight, and the extracting pressure that makes extraction kettle is 15Mpa; Extraction temperature is 36 ℃; CO
2Fluid flow rate is 60kg/h; The extraction time is 6h;
3), entering separating still after the extraction separates: the internal pressure of separation reactor I is 8MP, and temperature is 36 ℃; Separation reactor I I internal pressure is 16MP, and temperature is 26 ℃;
4) oil of the camphor tree sesame after will separating and entrainment agent mixed solution are that the 19000 high speed centrifugation devices that change separate through rotating speed, obtain camphor tree sesame oil;
5) the camphor tree sesame oil after will separating is collected and is weighed.
Camphor tree sesame sporophore extract camphor tree sesame oil yield is 38.5% in the present embodiment.
Embodiment three:
Method for extracting antrodia cinnamomea oil from antrodia cinnamomea fruiting bodies may further comprise the steps:
1) earlier camphor tree sesame fruit body powder is broken to 250 purpose camphor tree sesame fruit body powders;
2) supercritical CO of packing into of the camphor tree sesame fruit body powder after will pulverizing
2In the extraction kettle of extraction plant, and add the alcohol-water mixed solution as entrainment agent, the ratio of alcohol-water is: ethanol: water=1: 5, the weight of alcohol-water mixed solution are 21% of camphor tree sesame fruit body powder weight, and the extracting pressure that makes extraction kettle is 24Mpa; Extraction temperature is 55 ℃; CO
2Fluid flow rate is 85kg/h; The extraction time is 12h;
3), entering separating still after the extraction separates: the internal pressure of separation reactor I is 20MP, and temperature is 48 ℃; Separation reactor I I internal pressure is 13MP, and temperature is 38 ℃;
4) oil of the camphor tree sesame after will separating and entrainment agent mixed solution are that the 38000 high speed centrifugation devices that change separate through rotating speed, obtain camphor tree sesame oil;
5) the camphor tree sesame oil after will separating is collected and is weighed.
Camphor tree sesame sporophore extract camphor tree sesame oil yield is 39.2% in the present embodiment.
Embodiment four:
Method for extracting antrodia cinnamomea oil from antrodia cinnamomea fruiting bodies may further comprise the steps:
1) earlier camphor tree sesame fruit body powder is broken to 499 purpose camphor tree sesame fruit body powders;
2) supercritical CO of packing into of the camphor tree sesame fruit body powder after will pulverizing
2In the extraction kettle of extraction plant, and add the alcohol-water mixed solution as entrainment agent, the ratio of alcohol-water is: ethanol: water=1: 9.9, the weight of alcohol-water mixed solution are 39% of camphor tree sesame fruit body powder weight, and the extracting pressure that makes extraction kettle is 34Mpa; Extraction temperature is 64 ℃; CO
2Fluid flow rate is 115kg/h; The extraction time is 48h;
3), entering separating still after the extraction separates: the internal pressure of separation reactor I is 26MP, and temperature is 65 ℃; Separation reactor I I internal pressure is 15MP, and temperature is 50 ℃;
4) oil of the camphor tree sesame after will separating and entrainment agent mixed solution are that the 49000 high speed centrifugation devices that change separate through rotating speed, obtain camphor tree sesame oil;
5) the camphor tree sesame oil after will separating is collected and is weighed.
Camphor tree sesame sporophore extract camphor tree sesame oil yield is 40% in the present embodiment.
The above, it only is a kind of preferred embodiment of method for extracting antrodia cinnamomea oil from antrodia cinnamomea fruiting bodies of the present invention, be not that technical scope of the present invention is imposed any restrictions, every foundation technical spirit of the present invention all still belongs in the scope of technical solution of the present invention above any trickle modification, equivalent variations and modification that embodiment did.
Claims (10)
1. method for extracting antrodia cinnamomea oil from antrodia cinnamomea fruiting bodies, this method may further comprise the steps:
1) earlier camphor tree sesame fruit body powder is broken to 81～499 purpose camphor tree sesame fruit body powders;
2) supercritical CO of packing into of the camphor tree sesame fruit body powder after will pulverizing
2In the extraction kettle of extraction plant, add the alcohol-water mixed solution as entrainment agent, the extracting pressure of extraction kettle is 11MPa～36Mpa; Extraction temperature is 29 ℃～64 ℃; CO
2Fluid flow rate is 35～115kg/h; The extraction time is 0.5h～48h;
3), enter separating still and separate after the extraction, the internal pressure of separation reactor I is 6MP～26MP, and temperature is 31 ℃～65 ℃; Separation reactor I I internal pressure is 4MP～15MP, and temperature is 20 ℃～50 ℃, obtains camphor tree sesame oil and entrainment agent mixed solution;
4) oil of the camphor tree sesame after will separating and entrainment agent mixed solution are that 6000～49000 centrifugal devices that change separate through rotating speed, obtain camphor tree sesame oil;
5) the camphor tree sesame oil after will separating is collected and is weighed.
2. method for extracting antrodia cinnamomea oil from antrodia cinnamomea fruiting bodies according to claim 1 is characterized in that the proportional range of alcohol-water in the described entrainment agent is: 9.99: 0.01～1: 9.
3. method for extracting antrodia cinnamomea oil from antrodia cinnamomea fruiting bodies according to claim 1, the weight that it is characterized in that described entrainment agent are 1%～39% of camphor tree sesame fruit body powder weight.
4. method for extracting antrodia cinnamomea oil from antrodia cinnamomea fruiting bodies according to claim 1, the weight that it is characterized in that described entrainment agent are 0.1%～9.99% of camphor tree sesame fruit body powder weight.
5. method for extracting antrodia cinnamomea oil from antrodia cinnamomea fruiting bodies according to claim 1, the weight that it is characterized in that described entrainment agent are 20.1%～40% of camphor tree sesame fruit body powder weight.
6. method for extracting antrodia cinnamomea oil from antrodia cinnamomea fruiting bodies according to claim 1 is characterized in that the yield of described camphor tree sesame sporophore extract camphor tree sesame oil is 20%～40%.
7. method for extracting antrodia cinnamomea oil from antrodia cinnamomea fruiting bodies according to claim 1 is characterized in that described high speed centrifugation device rotary speed is 40001～49000 commentaries on classics.
8. method for extracting antrodia cinnamomea oil from antrodia cinnamomea fruiting bodies according to claim 1, the extracting pressure that it is characterized in that described extraction kettle is 11MPa～15Mpa, extraction temperature is 30 ℃～35 ℃.
9. method for extracting antrodia cinnamomea oil from antrodia cinnamomea fruiting bodies according to claim 1 is characterized in that described CO
2Fluid flow rate is 35～60kg/h.
10. method for extracting antrodia cinnamomea oil from antrodia cinnamomea fruiting bodies according to claim 1 is characterized in that the described extraction time is 3h～48h.
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| CN103923743A (en) * | 2013-05-09 | 2014-07-16 | 刘立 | Extraction method for effective compositions of antrodia camphorata |
| CN104327942A (en) * | 2014-10-13 | 2015-02-04 | 中山安荞生物科技有限公司 | Method for extracting antrodia camphorata oil from antrodia camphorata mycelium |
| CN104327943A (en) * | 2014-10-13 | 2015-02-04 | 中山安荞生物科技有限公司 | Method for extracting antrodia camphorata oil from antrodia camphorata sporophore |
| TWI574693B (en) * | 2015-03-06 | 2017-03-21 | Separation and Purification of Functional Components in Antrodia camphora by Supercritical Fluid Technology | |
| CN104856238A (en) * | 2015-05-15 | 2015-08-26 | 深圳市杰仕博科技有限公司 | Indirect heating type atomizer |
| CN108329999A (en) * | 2018-02-09 | 2018-07-27 | 平潭迈康生物科技有限公司 | A kind of extracting method of Antrodia camphorata essential oil and its application in teenager's health care |
| CN108456592A (en) * | 2018-02-09 | 2018-08-28 | 平潭迈康生物科技有限公司 | A kind of extracting method of Antrodia camphorata essential oil and its application in cosmetics |
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| CN1347732A (en) * | 2001-09-28 | 2002-05-08 | 陈顺志 | Supercritical extraction process of producing cordyceps oil |
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