CN101993480B - Broccoli leaf protein and preparation method thereof - Google Patents
Broccoli leaf protein and preparation method thereof Download PDFInfo
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- CN101993480B CN101993480B CN 200910091131 CN200910091131A CN101993480B CN 101993480 B CN101993480 B CN 101993480B CN 200910091131 CN200910091131 CN 200910091131 CN 200910091131 A CN200910091131 A CN 200910091131A CN 101993480 B CN101993480 B CN 101993480B
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- caulis
- folium brassicae
- brassicae capitatae
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Abstract
The invention discloses a broccoli leaf protein and a preparation method thereof. The broccoli leaf protein is prepared according to the following steps of: preparing juice by using at least one organ of stems, leaves and ball-flowers of the broccoli as a raw material; and carrying out acid treatment and heat treatment, or, carrying out acid treatment and flocculant treatment to on the juice to obtain a sediment which is broccoli leaf protein. The broccoli leaf protein is dark-green powder, and has special taste of the broccoli, crude protein content of not less than 40 percent and the total amino acid content of not less than 25 percent. The broccoli leaf protein can be used in the feed for replenishing the protein required by animals.
Description
Technical field
The present invention relates to a kind of Caulis et Folium Brassicae capitatae leaf protein and preparation method thereof.
Background technology
Caulis et Folium Brassicae capitatae is the stronger vegetable crop of a kind of seasonality, and its bouquet collecting time concentrated between annual March November to next year, and the plant cauline leaf behind the bouquet of gathering is stacked the field as waste, severe contamination Caulis et Folium Brassicae capitatae main producing region environment.According to measuring and calculating, the general per mu yield of broccoli stem-leaf is 2.5～3 tons, only calculates with 70,000 mu of Caulis et Folium Brassicae capitatae planting bases, Linhai City Shang Pan town, Zhejiang Province, and its total amount can reach 17.5～21.0 ten thousand tons.The broccoli stem-leaf that undue concentration is piled up has a strong impact on the benign cycle of plough horizon ecotope, the nitrogen, the phosphoric that produce after broccoli stem-leaf after the excessive also field second ploughing rots cause body eutrophication, cause cultured fishes and other aquatic organism mass mortalities.Produce a large amount of sewage and foul gass such as ammonia, hydrogen sulfide after the scrap stock that produce when a large amount of blades that produce and cutting bouquet rot in the collecting and distributing course of processing of Caulis et Folium Brassicae capitatae, have a strong impact on the healthy and quality of life of peripheral resident.Therefore, the improvement of broccoli stem-leaf the like waste is for ensureing that the Sustainable development of Caulis et Folium Brassicae capitatae industry is significant.
Along with developing rapidly of livestock industry, the problem of protein feed supply wretched insufficiency becomes increasingly conspicuous.Protein feed resource is all actively being sought to develop in countries in the world, and wherein developing the protein resource of containing in the green plants becomes one of important channel of current acquisition high-quality, cheap protein provision.Studies show that, contain rich in protein in the broccoli stem-leaf, after processing such as squeezing, can from its juice, extract and obtain the leaf protein product.Though the method for extracting leaf protein in other plant such as clover, the Chinese scholartree leaf has been had more research, because physics and the chemical property of different vegetable-proteins have larger difference, existing extracting method is not suitable for and extracts the Caulis et Folium Brassicae capitatae leaf protein.
Summary of the invention
The purpose of this invention is to provide a kind of Caulis et Folium Brassicae capitatae and preparation method thereof.
Caulis et Folium Brassicae capitatae provided by the present invention is the Caulis et Folium Brassicae capitatae leaf protein, according to the preparation of following method: be feedstock production juice with at least a organ in stem, leaf and three kinds of organs of bouquet of Caulis et Folium Brassicae capitatae, described juice is carried out in following two kinds of processing after any handles, and the throw out that obtains is the Caulis et Folium Brassicae capitatae leaf protein:
1) carry out acid and thermal treatment successively,
2) carrying out acid and flocculation agent successively handles.
The acid-treated pH of described juice is 2.0-5.0, and heat treated temperature is 60-90 ℃, and soaking time is 5-10 minute; The pH that described acid and flocculation agent are handled is 2.0-5.0, and flocculation agent is sodium polyacrylate, and consumption 0.05-0.2%, flocculation time are 5-30 minute.
Wherein, the acid-treated pH of described juice is preferably 3.0, and thermal treatment temp is preferably 70 ℃, and soaking time is preferably 9 minutes; Described juice is preferably 3.5 through the pH of acid and flocculation agent processing, and the flocculation agent consumption is preferably 0.10%, and flocculation time is preferably 15 minutes.
Described raw material is from arbitrary phase or each the phase arbitrary combination of squaring period, bouquet vegetative period and bouquet harvesting time.
Zhi Bei Caulis et Folium Brassicae capitatae leaf protein is dark green or shallow green powder according to the method described above, has the special odor of Caulis et Folium Brassicae capitatae, and the content of crude protein is more than or equal to 40%, and the content of total amino acid is more than or equal to 25%.
The present invention has the following advantages: the orchid production waste is raw material 1,, has reduced production cost, has alleviated environmental pollution, has remarkable economical and ecological benefits.2, be raw material with the edible vegetable crop of the mankind, its Product Safety is secure.3, product contains rich in amino acid, and the protein raw material that can be used as high-quality substitutes dregs of beans, fish meal etc., solves the problem of high-quality protein feed inadequate resource.
Caulis et Folium Brassicae capitatae leaf protein of the present invention can be used for various domestic animals, poultry, aquatic animal and other cultivated animals feeds, replenishes the required protein of animal.
Embodiment
The Caulis et Folium Brassicae capitatae leaf protein is extracted in heating under embodiment 1, the acidic conditions
1, raw materials pretreatment
With 100kg bouquet Caulis et Folium Brassicae capitatae harvesting time stem, the leaf of collecting, feeder greens pulverizer or hay cutter are ground into the long chip of 1-2cm, with the hollander making beating, through the expeller squeezing, cross the filter screen of aperture 2.0mm and remove, and collect the juice 51.4kg that squeezes.Record dry-matter in the juice, crude protein and total amino acid content and see Table 1.
Table 1 Caulis et Folium Brassicae capitatae leaf sap nutrition content measurement result
| Index | Measured value | Measuring method |
| Dry-matter | 2.61％ | GB/T 6435-2006 |
| Crude protein | 1.36％ | GB/T 6432-1994 |
| Total amino acid | 0.83％ | GB/T 18246-2000 |
2, the extraction of Caulis et Folium Brassicae capitatae leaf protein
1) in above-mentioned juice, adds hydrochloric acid, regulate juice pH to 3.0, with heat exchanger heats to 70 ℃, be incubated 9 minutes, leave standstill cooling;
2) filter with the filter bag of industrial filter cloth making or with pressure filter, pore size filter is 74 μ m (250 orders), and the filter residue that obtains is adopted the oven dry of drying under reduced pressure machine, obtains the Caulis et Folium Brassicae capitatae leaf protein, and weight is 1.03kg.The Caulis et Folium Brassicae capitatae leaf protein that extraction is obtained detects, and the results are shown in Table 2.
Table 2 Caulis et Folium Brassicae capitatae leaf protein nutrition content measurement result
| Index | Measured value | Measuring method |
| Moisture | 12.11％ | GB/T 6435-2006 |
| Crude protein | 56.81％ | GB/T 6432-1994 |
| Coarse ash | 3.01％ | GB/T 6438-2007 |
| Robust fibre | 1.43％ | GB/T 6434-1994 |
| Crude fat | 2.11％ | GB/T 6433-2006 |
| Nitrogen-free extract | 24.53％ | Calculated value |
| Add up to | 100％ | - |
| Wherein, total amino acid | 34.78％ | GB/T 18246-2000 |
Calculate, this method is 67.48% to the extraction yield of dry-matter in the Caulis et Folium Brassicae capitatae juice, and the crude protein extraction yield is 83.71%, and the total amino acid extraction yield is 83.97%.
Add flocculation agent under embodiment 2, the acidic conditions and extract the Caulis et Folium Brassicae capitatae leaf protein
1, raw materials pretreatment
With 100kg bouquet Caulis et Folium Brassicae capitatae harvesting time stem, the leaf of collecting, feeder greens pulverizer or hay cutter are ground into the long chip of 1-2cm, with the hollander making beating, through the expeller squeezing, cross the filter screen of aperture 2.0mm and remove, and collect the juice 53.2kg that squeezes.Record dry-matter in the juice, crude protein and total amino acid content and see Table 3.
Table 3 Caulis et Folium Brassicae capitatae leaf sap nutrition content measurement result
| Index | Measured value | Measuring method |
| Dry-matter | 2.78％ | GB/T 6435-2006 |
| Crude protein | 1.44％ | GB/T 6432-1994 |
| Total amino acid | 0.81％ | GB/T 18246-2000 |
2, the extraction of Caulis et Folium Brassicae capitatae leaf protein
1) in above-mentioned juice, adds hydrochloric acid, regulate juice pH to 3.5;
2) in juice, add 0.5% sodium polyacrylate solution 20mL by every 1000mL, left standstill 15 minutes;
3) filter with the filter bag of industrial filter cloth making or with pressure filter, pore size filter is 74 μ m (250 orders), and the filter residue that obtains is adopted the oven dry of drying under reduced pressure machine, obtains the Caulis et Folium Brassicae capitatae leaf protein, and weight is 1.15kg.The Caulis et Folium Brassicae capitatae leaf protein that extraction is obtained detects, and the results are shown in Table 4.
Table 4 Caulis et Folium Brassicae capitatae leaf protein nutrition content measurement result
| Index | Measured value | Measuring method |
| Moisture | 12.72％ | GB/T 6435-2006 |
| Crude protein | 55.43％ | GB/T 6432-1994 |
| Coarse ash | 3.43％ | GB/T 6438-2007 |
| Robust fibre | 1.21％ | GB/T 6434-1994 |
| Crude fat | 2.00％ | GB/T 6433-2006 |
| Nitrogen-free extract | 25.21％ | Calculated value |
| Add up to | 100％ | - |
| Wherein, total amino acid | 32.89％ | GB/T 18246-2000 |
Calculate, this method is 67.87% to the extraction yield of dry-matter in the Caulis et Folium Brassicae capitatae juice, and the crude protein extraction yield is 83.21%, and the total amino acid extraction yield is 87.78%.
Claims (4)
1. the preparation method of a Caulis et Folium Brassicae capitatae is that at least a organ is feedstock production juice in stem, leaf and the three kinds of organs of bouquet with Caulis et Folium Brassicae capitatae, described juice is carried out successively acid and flocculation agent processing after, the throw out that obtains is Caulis et Folium Brassicae capitatae:
Described juice is carried out in acid and the flocculation agent processing, and described acid-treated pH is 2.0-5.0, and flocculation agent is sodium polyacrylate, and consumption is 0.05-0.2%, and flocculation time is 5-30 minute.
2. method according to claim 2 is characterized in that: described juice is carried out during acid treatment, flocculation agent handle, and described acid-treated pH is 3.5, and the consumption of flocculation agent is 0.1%, and flocculation time is 15 minutes.
3. method according to claim 1 and 2 is characterized in that: described raw material is from arbitrary phase or its arbitrary combination of squaring period, bouquet vegetative period and bouquet harvesting time.
4. according to the Caulis et Folium Brassicae capitatae of arbitrary described method preparation among the claim 1-3, it is characterized in that: in the described Caulis et Folium Brassicae capitatae, the content of crude protein is more than or equal to 40%, and total amino acid content is more than or equal to 25%.
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| CN104356194B (en) * | 2014-10-27 | 2019-05-10 | 大兴安岭林格贝寒带生物科技股份有限公司 | The method for extracting blueberry albumen in blueberry using inorganic acid |
| CN106135630A (en) * | 2015-04-10 | 2016-11-23 | 浙江海正药业股份有限公司 | A kind of preparation method and its usage of Caulis et Folium Brassicae capitatae egg albumen powder |
| CN106191183B (en) | 2015-05-04 | 2019-11-29 | 浙江海正药业股份有限公司 | The preparation method and applications of broccoli protein peptides |
| CN106256250A (en) * | 2015-06-15 | 2016-12-28 | 浙江海正药业股份有限公司 | A kind of Caulis et Folium Brassicae capitatae hatches the preparation method of powder |
| CN107373225A (en) * | 2017-07-19 | 2017-11-24 | 郝之奎 | A kind of broccoli protein peptide drinks and preparation method thereof |
| CN107365347A (en) * | 2017-07-19 | 2017-11-21 | 郝之奎 | A kind of extracting method of broccoli protein peptides |
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| CN101248836B (en) * | 2008-03-21 | 2010-09-15 | 浙江大学 | Method for utilizing squeezing juice of broccoli stem and leaf as animal feedstuffs |
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