CN101993480B - Broccoli leaf protein and preparation method thereof - Google Patents
Broccoli leaf protein and preparation method thereof Download PDFInfo
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- CN101993480B CN101993480B CN 200910091131 CN200910091131A CN101993480B CN 101993480 B CN101993480 B CN 101993480B CN 200910091131 CN200910091131 CN 200910091131 CN 200910091131 A CN200910091131 A CN 200910091131A CN 101993480 B CN101993480 B CN 101993480B
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- 235000011299 Brassica oleracea var botrytis Nutrition 0.000 title claims abstract description 18
- 235000017647 Brassica oleracea var italica Nutrition 0.000 title claims abstract description 18
- 238000002360 preparation method Methods 0.000 title claims abstract description 7
- 101710138460 Leaf protein Proteins 0.000 title abstract description 25
- 244000308180 Brassica oleracea var. italica Species 0.000 title 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims abstract description 23
- 240000003259 Brassica oleracea var. botrytis Species 0.000 claims abstract description 17
- 238000000034 method Methods 0.000 claims abstract description 13
- 150000001413 amino acids Chemical class 0.000 claims abstract description 12
- 235000019750 Crude protein Nutrition 0.000 claims abstract description 11
- 239000002994 raw material Substances 0.000 claims abstract description 9
- 238000010306 acid treatment Methods 0.000 claims abstract description 6
- 210000000056 organ Anatomy 0.000 claims abstract description 5
- 238000011282 treatment Methods 0.000 claims abstract 5
- 239000002244 precipitate Substances 0.000 claims abstract 2
- 238000005189 flocculation Methods 0.000 claims description 10
- 230000016615 flocculation Effects 0.000 claims description 10
- 239000000284 extract Substances 0.000 claims description 9
- 239000002253 acid Substances 0.000 claims description 8
- 238000003306 harvesting Methods 0.000 claims description 4
- 229920001495 poly(sodium acrylate) polymer Polymers 0.000 claims description 3
- NNMHYFLPFNGQFZ-UHFFFAOYSA-M sodium polyacrylate Chemical group [Na+].[O-]C(=O)C=C NNMHYFLPFNGQFZ-UHFFFAOYSA-M 0.000 claims description 3
- 230000034303 cell budding Effects 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 abstract description 9
- 102000004169 proteins and genes Human genes 0.000 abstract description 9
- 241001465754 Metazoa Species 0.000 abstract description 5
- 238000010438 heat treatment Methods 0.000 abstract description 2
- 239000000843 powder Substances 0.000 abstract description 2
- 239000013589 supplement Substances 0.000 abstract 1
- 238000000605 extraction Methods 0.000 description 8
- 239000003795 chemical substances by application Substances 0.000 description 6
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 235000016709 nutrition Nutrition 0.000 description 4
- 230000035764 nutrition Effects 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 2
- 238000010009 beating Methods 0.000 description 2
- 235000019784 crude fat Nutrition 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 239000004744 fabric Substances 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 235000021384 green leafy vegetables Nutrition 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 238000000751 protein extraction Methods 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- 238000007669 thermal treatment Methods 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 108010082495 Dietary Plant Proteins Proteins 0.000 description 1
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 1
- 235000019733 Fish meal Nutrition 0.000 description 1
- 241000233855 Orchidaceae Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 244000046101 Sophora japonica Species 0.000 description 1
- 235000010586 Sophora japonica Nutrition 0.000 description 1
- 241000219793 Trifolium Species 0.000 description 1
- 241001464837 Viridiplantae Species 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 239000004467 fishmeal Substances 0.000 description 1
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- Peptides Or Proteins (AREA)
Abstract
本发明公开了一种西兰花叶蛋白及其制备方法。该西兰花叶蛋白按照下述方法制备：以西兰花的茎、叶和花球三种器官中至少一种器官为原料制备汁液，将所述汁液进行下述两种处理中任一种处理后，得到的沉淀物即为西兰花叶蛋白：1)进行酸处理、热处理，2)进行酸处理、絮凝剂处理。本发明的西兰花叶蛋白为深绿色粉末，具西兰花特殊气味，粗蛋白质含量不低于40％，总氨基酸含量不低于25％。本发明所提供的西兰花叶蛋白可用于饲料，补充动物所需的蛋白质。The invention discloses broccoli leaf protein and a preparation method thereof. The broccoli leaf protein is prepared according to the following method: at least one of the three organs of broccoli stems, leaves and curds is used as a raw material to prepare juice, and after the juice is subjected to any one of the following two treatments, The obtained precipitate is broccoli leaf protein: 1) acid treatment and heat treatment, 2) acid treatment and flocculant treatment. The broccoli leaf protein of the invention is a dark green powder with a special smell of broccoli, the crude protein content is not less than 40%, and the total amino acid content is not less than 25%. The broccoli leaf protein provided by the invention can be used in feed to supplement protein needed by animals.
Description
Technical field
The present invention relates to a kind of Caulis et Folium Brassicae capitatae leaf protein and preparation method thereof.
Background technology
Caulis et Folium Brassicae capitatae is the stronger vegetable crop of a kind of seasonality, and its bouquet collecting time concentrated between annual March November to next year, and the plant cauline leaf behind the bouquet of gathering is stacked the field as waste, severe contamination Caulis et Folium Brassicae capitatae main producing region environment.According to measuring and calculating, the general per mu yield of broccoli stem-leaf is 2.5～3 tons, only calculates with 70,000 mu of Caulis et Folium Brassicae capitatae planting bases, Linhai City Shang Pan town, Zhejiang Province, and its total amount can reach 17.5～21.0 ten thousand tons.The broccoli stem-leaf that undue concentration is piled up has a strong impact on the benign cycle of plough horizon ecotope, the nitrogen, the phosphoric that produce after broccoli stem-leaf after the excessive also field second ploughing rots cause body eutrophication, cause cultured fishes and other aquatic organism mass mortalities.Produce a large amount of sewage and foul gass such as ammonia, hydrogen sulfide after the scrap stock that produce when a large amount of blades that produce and cutting bouquet rot in the collecting and distributing course of processing of Caulis et Folium Brassicae capitatae, have a strong impact on the healthy and quality of life of peripheral resident.Therefore, the improvement of broccoli stem-leaf the like waste is for ensureing that the Sustainable development of Caulis et Folium Brassicae capitatae industry is significant.
Along with developing rapidly of livestock industry, the problem of protein feed supply wretched insufficiency becomes increasingly conspicuous.Protein feed resource is all actively being sought to develop in countries in the world, and wherein developing the protein resource of containing in the green plants becomes one of important channel of current acquisition high-quality, cheap protein provision.Studies show that, contain rich in protein in the broccoli stem-leaf, after processing such as squeezing, can from its juice, extract and obtain the leaf protein product.Though the method for extracting leaf protein in other plant such as clover, the Chinese scholartree leaf has been had more research, because physics and the chemical property of different vegetable-proteins have larger difference, existing extracting method is not suitable for and extracts the Caulis et Folium Brassicae capitatae leaf protein.
Summary of the invention
The purpose of this invention is to provide a kind of Caulis et Folium Brassicae capitatae and preparation method thereof.
Caulis et Folium Brassicae capitatae provided by the present invention is the Caulis et Folium Brassicae capitatae leaf protein, according to the preparation of following method: be feedstock production juice with at least a organ in stem, leaf and three kinds of organs of bouquet of Caulis et Folium Brassicae capitatae, described juice is carried out in following two kinds of processing after any handles, and the throw out that obtains is the Caulis et Folium Brassicae capitatae leaf protein:
1) carry out acid and thermal treatment successively,
2) carrying out acid and flocculation agent successively handles.
The acid-treated pH of described juice is 2.0-5.0, and heat treated temperature is 60-90 ℃, and soaking time is 5-10 minute; The pH that described acid and flocculation agent are handled is 2.0-5.0, and flocculation agent is sodium polyacrylate, and consumption 0.05-0.2%, flocculation time are 5-30 minute.
Wherein, the acid-treated pH of described juice is preferably 3.0, and thermal treatment temp is preferably 70 ℃, and soaking time is preferably 9 minutes; Described juice is preferably 3.5 through the pH of acid and flocculation agent processing, and the flocculation agent consumption is preferably 0.10%, and flocculation time is preferably 15 minutes.
Described raw material is from arbitrary phase or each the phase arbitrary combination of squaring period, bouquet vegetative period and bouquet harvesting time.
Zhi Bei Caulis et Folium Brassicae capitatae leaf protein is dark green or shallow green powder according to the method described above, has the special odor of Caulis et Folium Brassicae capitatae, and the content of crude protein is more than or equal to 40%, and the content of total amino acid is more than or equal to 25%.
The present invention has the following advantages: the orchid production waste is raw material 1,, has reduced production cost, has alleviated environmental pollution, has remarkable economical and ecological benefits.2, be raw material with the edible vegetable crop of the mankind, its Product Safety is secure.3, product contains rich in amino acid, and the protein raw material that can be used as high-quality substitutes dregs of beans, fish meal etc., solves the problem of high-quality protein feed inadequate resource.
Caulis et Folium Brassicae capitatae leaf protein of the present invention can be used for various domestic animals, poultry, aquatic animal and other cultivated animals feeds, replenishes the required protein of animal.
Embodiment
The Caulis et Folium Brassicae capitatae leaf protein is extracted in heating under embodiment 1, the acidic conditions
1, raw materials pretreatment
With 100kg bouquet Caulis et Folium Brassicae capitatae harvesting time stem, the leaf of collecting, feeder greens pulverizer or hay cutter are ground into the long chip of 1-2cm, with the hollander making beating, through the expeller squeezing, cross the filter screen of aperture 2.0mm and remove, and collect the juice 51.4kg that squeezes.Record dry-matter in the juice, crude protein and total amino acid content and see Table 1.
Table 1 Caulis et Folium Brassicae capitatae leaf sap nutrition content measurement result
| Index | Measured value | Measuring method |
| Dry-matter | 2.61％ | GB/T 6435-2006 |
| Crude protein | 1.36％ | GB/T 6432-1994 |
| Total amino acid | 0.83％ | GB/T 18246-2000 |
2, the extraction of Caulis et Folium Brassicae capitatae leaf protein
1) in above-mentioned juice, adds hydrochloric acid, regulate juice pH to 3.0, with heat exchanger heats to 70 ℃, be incubated 9 minutes, leave standstill cooling;
2) filter with the filter bag of industrial filter cloth making or with pressure filter, pore size filter is 74 μ m (250 orders), and the filter residue that obtains is adopted the oven dry of drying under reduced pressure machine, obtains the Caulis et Folium Brassicae capitatae leaf protein, and weight is 1.03kg.The Caulis et Folium Brassicae capitatae leaf protein that extraction is obtained detects, and the results are shown in Table 2.
Table 2 Caulis et Folium Brassicae capitatae leaf protein nutrition content measurement result
| Index | Measured value | Measuring method |
| Moisture | 12.11％ | GB/T 6435-2006 |
| Crude protein | 56.81％ | GB/T 6432-1994 |
| Coarse ash | 3.01％ | GB/T 6438-2007 |
| Robust fibre | 1.43％ | GB/T 6434-1994 |
| Crude fat | 2.11％ | GB/T 6433-2006 |
| Nitrogen-free extract | 24.53％ | Calculated value |
| Add up to | 100％ | - |
| Wherein, total amino acid | 34.78％ | GB/T 18246-2000 |
Calculate, this method is 67.48% to the extraction yield of dry-matter in the Caulis et Folium Brassicae capitatae juice, and the crude protein extraction yield is 83.71%, and the total amino acid extraction yield is 83.97%.
Add flocculation agent under embodiment 2, the acidic conditions and extract the Caulis et Folium Brassicae capitatae leaf protein
1, raw materials pretreatment
With 100kg bouquet Caulis et Folium Brassicae capitatae harvesting time stem, the leaf of collecting, feeder greens pulverizer or hay cutter are ground into the long chip of 1-2cm, with the hollander making beating, through the expeller squeezing, cross the filter screen of aperture 2.0mm and remove, and collect the juice 53.2kg that squeezes.Record dry-matter in the juice, crude protein and total amino acid content and see Table 3.
Table 3 Caulis et Folium Brassicae capitatae leaf sap nutrition content measurement result
| Index | Measured value | Measuring method |
| Dry-matter | 2.78％ | GB/T 6435-2006 |
| Crude protein | 1.44％ | GB/T 6432-1994 |
| Total amino acid | 0.81％ | GB/T 18246-2000 |
2, the extraction of Caulis et Folium Brassicae capitatae leaf protein
1) in above-mentioned juice, adds hydrochloric acid, regulate juice pH to 3.5;
2) in juice, add 0.5% sodium polyacrylate solution 20mL by every 1000mL, left standstill 15 minutes;
3) filter with the filter bag of industrial filter cloth making or with pressure filter, pore size filter is 74 μ m (250 orders), and the filter residue that obtains is adopted the oven dry of drying under reduced pressure machine, obtains the Caulis et Folium Brassicae capitatae leaf protein, and weight is 1.15kg.The Caulis et Folium Brassicae capitatae leaf protein that extraction is obtained detects, and the results are shown in Table 4.
Table 4 Caulis et Folium Brassicae capitatae leaf protein nutrition content measurement result
| Index | Measured value | Measuring method |
| Moisture | 12.72％ | GB/T 6435-2006 |
| Crude protein | 55.43％ | GB/T 6432-1994 |
| Coarse ash | 3.43％ | GB/T 6438-2007 |
| Robust fibre | 1.21％ | GB/T 6434-1994 |
| Crude fat | 2.00％ | GB/T 6433-2006 |
| Nitrogen-free extract | 25.21％ | Calculated value |
| Add up to | 100％ | - |
| Wherein, total amino acid | 32.89％ | GB/T 18246-2000 |
Calculate, this method is 67.87% to the extraction yield of dry-matter in the Caulis et Folium Brassicae capitatae juice, and the crude protein extraction yield is 83.21%, and the total amino acid extraction yield is 87.78%.
Claims (4)
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| CN 200910091131 CN101993480B (en) | 2009-08-12 | 2009-08-12 | Broccoli leaf protein and preparation method thereof |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104356194B (en) * | 2014-10-27 | 2019-05-10 | 大兴安岭林格贝寒带生物科技股份有限公司 | The method for extracting blueberry albumen in blueberry using inorganic acid |
| CN106135630A (en) * | 2015-04-10 | 2016-11-23 | 浙江海正药业股份有限公司 | A kind of preparation method and its usage of Caulis et Folium Brassicae capitatae egg albumen powder |
| CN106191183B (en) * | 2015-05-04 | 2019-11-29 | 浙江海正药业股份有限公司 | The preparation method and applications of broccoli protein peptides |
| CN106256250A (en) * | 2015-06-15 | 2016-12-28 | 浙江海正药业股份有限公司 | A kind of Caulis et Folium Brassicae capitatae hatches the preparation method of powder |
| CN107373225A (en) * | 2017-07-19 | 2017-11-24 | 郝之奎 | A kind of broccoli protein peptide drinks and preparation method thereof |
| CN107365347A (en) * | 2017-07-19 | 2017-11-21 | 郝之奎 | A kind of extracting method of broccoli protein peptides |
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| KR20080018929A (en) * | 2008-02-09 | 2008-02-28 | 주식회사 유니바이오 | Preparation method of feed additive using broccoli |
| CN101248836B (en) * | 2008-03-21 | 2010-09-15 | 浙江大学 | Method for Feed Utilization of Broccoli Stem and Leaf Pressed Juice |
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