CN106135630A - A kind of preparation method and its usage of Caulis et Folium Brassicae capitatae egg albumen powder - Google Patents
A kind of preparation method and its usage of Caulis et Folium Brassicae capitatae egg albumen powder Download PDFInfo
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- CN106135630A CN106135630A CN201510167335.7A CN201510167335A CN106135630A CN 106135630 A CN106135630 A CN 106135630A CN 201510167335 A CN201510167335 A CN 201510167335A CN 106135630 A CN106135630 A CN 106135630A
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- folium brassicae
- brassicae capitatae
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Abstract
The present invention relates to a kind of method preparing Caulis et Folium Brassicae capitatae egg albumen powder, described method includes: will be prepared as juice with at least one organ in stem, leaf and three kinds of organs of bouquet of Caulis et Folium Brassicae capitatae for raw material, heating flocculation juice, it is filtrated to get solid protein and filtrate, filtrate mixes with solid protein and homogenizing after membrance concentration again, then obtains Caulis et Folium Brassicae capitatae egg albumen powder by spray drying.The method technique of the present invention is simple, workable, it is demonstrated experimentally that the Caulis et Folium Brassicae capitatae egg albumen powder that the present invention obtains has reduces the effect of cholesterol in blood fat, can be used in the fields such as food, health product and medicine.
Description
Technical field
The present invention relates to the preparation method of a kind of Caulis et Folium Brassicae capitatae egg albumen powder, and the Caulis et Folium Brassicae capitatae egg albumen powder prepared gallbladder in reducing blood fat is solid
Purposes in alcohol.
Background technology
Caulis et Folium Brassicae capitatae (Brassica oleracea var.italia) is Cruciferae Btassica Caulis et Folium Brassicae capitatae mutation, and Appennin sausage contains
Abundant bioactive substance and nutrient substance, be described as " vegetable Phaleria macrocarpa ".Nutritional labeling in Caulis et Folium Brassicae capitatae, not only content is high, and
Very comprehensive, mainly include protein, carbohydrate, fat, mineral, vitamin C and carotene etc., wherein vitamin
C content exceeds cabbage rolls about 20%, is 23 times of cabbage, is 56 times of Fructus Lycopersici esculenti；The content of carotene is cabbage rolls
More than 30 times；Folate content is 2 times of cabbage rolls；Protein content is 3 times of cabbage rolls, 4 times of Fructus Lycopersici esculenti.Additionally, in Caulis et Folium Brassicae capitatae
Than other vegetable more comprehensively, calcium, phosphorus, ferrum, potassium, zinc, manganese equal size are the abundantest, and ratio belongs to Cruciferae for mineral composition
Cabbage rolls exceeds a lot.
Taizhou is country's largest Caulis et Folium Brassicae capitatae production base and important international Caulis et Folium Brassicae capitatae export place, and its Caulis et Folium Brassicae capitatae yield accounts for Zhejiang
50%, the 25% of the whole nation.Caulis et Folium Brassicae capitatae leaves substantial amounts of broccoli stem-leaf after plucking, and it is a local multi-form agriculture pollution of area source.For solving
The pollution problem that broccoli stem-leaf produces, domestic enterprise develops the drying feedstuff of a kind of stem and leaf powder, and it is by pulverizing, squeezing and dry
Obtain broccoli stem-leaf powder, but the Sucus Caulis et Folium Brassicae capitatae of its squeezing generation still contains a large number of nutrients, be expelled directly out same to environment
Produce large effect.In Caulis et Folium Brassicae capitatae squeezing juice, solids content is only between 5-10%, if Direct spraying is dried or lyophilizing, and its cost
The highest.To this end, ZL200910091131.4 discloses a kind of broccoli leaf protein and preparation method thereof, it is heated by acid adjustment or adjusts
Acid adds the method flocculation Caulis et Folium Brassicae capitatae squeezing juice of flocculant and obtains the albumen that flocculates, and then obtains broccoli leaf protein by drying flocculation albumen.
The most of albumen in Caulis et Folium Brassicae capitatae squeezing juice although the method has flocculated, but its residue waste water still has the biggest pollution to environment；
On the other hand, the leaf protein prepared is owing to the addition of the additives such as flocculant, and it is not suitable for people and eats, and is only suitable for as animal
Feedstuff.Patent CN200710086583.4 provides one and utilizes membrane separation technique comprehensive utilization Caulis et Folium Brassicae capitatae (and cabbage rolls) stem and leaf squeezing
The method of juice.In the method, Caulis et Folium Brassicae capitatae squeezing juice directly carries out membrance concentration after simple pretreatment, then utilizes concentrated solution to produce
Fertilizer or feedstuff.The method is not suitable for industrialized production very much, and first, squeezing juice, through simple pre-filtering, is very easy to blocking fenestra,
It is difficult to carry out follow-up membrance concentration；Secondly because membrance concentration easily blocks, so processing time lengthening, squeezing juice is caused easily to become
Matter, it is impossible to ensure the quality of final products.
For above-mentioned produced problem, the invention provides a kind of method preparing Caulis et Folium Brassicae capitatae egg albumen powder.The method is on the one hand without appointing
What additive, the Caulis et Folium Brassicae capitatae egg albumen powder obtained has good water dispersible and dissolubility；Second, technique employs membrance concentration, green
Color is energy-conservation, produces almost without refuse, reaches green production standard；3rd, technological process is more scientific, is suitable for industrialized production.
Summary of the invention
For technological deficiency present in existing Caulis et Folium Brassicae capitatae egg albumen powder preparation technology, the invention provides one more green energy conservation, more
The preparation method of the Caulis et Folium Brassicae capitatae egg albumen powder of science.
The present invention relates to the preparation method of a kind of Caulis et Folium Brassicae capitatae egg albumen powder, comprising:
(1) it is prepared as juice with at least one organ in stem, leaf and three kinds of organs of bouquet of Caulis et Folium Brassicae capitatae for raw material,
(2) protein denaturation in step (1) gained juice is made to flocculate by heating,
(3) step (2) gained mixed liquor is filtered, obtains filter cake (flocculation albumen) and filtrate,
(4) optional, step (3) gained filtrate is cooled to less than 50 DEG C,
(5) step (3) or (4) gained filtrate are carried out membrance concentration process, obtain concentrated solution,
(6) by step (3) gained filter cake and step (5) gained concentrated solution mixing homogenizing,
(7) step (6) gained mixed liquor is further dried obtains Caulis et Folium Brassicae capitatae egg albumen powder.
Wherein, the heating-up temperature described in step (2) is 70-120 DEG C.
Wherein, the film described in step (5) be selected from reverse osmosis membrane, include but not limited to the SC series membranes of GE company, SG series membranes,
The BW30 series membranes of SE series membranes and the Dow Chemical Company, or NF membrane, include but not limited to the DK series membranes of GE company, DL series membranes
NF270 series membranes with the Dow Chemical Company.Wherein, the molecular cut off scope of described NF membrane is 100-500D, preferably 100-300D.
Owing to step (3) gained filtrate being contained a certain amount of little peptide molecule, therefore to the loss of this little peptide molecule during reducing membrance concentration,
Preferably reverse osmosis membrane concentrates.
Wherein, the drying means described in step (6) includes being spray-dried or lyophilization.
In the Caulis et Folium Brassicae capitatae juice that step (1) obtains, solids content is about 5-10%, if Direct spraying is dried or lyophilizing, its cost is very
Height, the solids content in the mixing homogenizing fluid before being finally dried can be controlled, at 20-40%, to be therefore substantially reduced by the method for the invention
Production cost.It addition, said method carries out membrance concentration to the filtrate after flocculation, then membrance concentration liquid is mixed with flocculation albumen
Homogenizing, is dried the most again, and this method is the most more environmentally friendly, and the Caulis et Folium Brassicae capitatae egg albumen powder obtained can also retain in Caulis et Folium Brassicae capitatae many
The more nutrient substance such as sugar.Test data shows, the Caulis et Folium Brassicae capitatae egg albumen powder that said method prepares has extraordinary reduction blood fat
The effect of middle cholesterol, therefore can apply in the fields such as food, health product and medicine.
The Caulis et Folium Brassicae capitatae egg albumen powder that said method prepares, on the one hand, be added without any additive, the Caulis et Folium Brassicae capitatae egg albumen powder obtained has
Well water dispersible and dissolubility；On the other hand, technique employs membrance concentration, green energy conservation, produces almost without refuse, reach
To green production standard, and technological process is more scientific, is suitable for industrialized production.
Present invention also offers the preparation method of another kind of Caulis et Folium Brassicae capitatae egg albumen powder, comprising:
(1) it is prepared as juice with at least one organ in stem, leaf and three kinds of organs of bouquet of Caulis et Folium Brassicae capitatae for raw material,
(2) protein denaturation in step (1) gained juice is made to flocculate by heating,
(3) step (2) gained mixed liquor is filtered, obtains filter cake (flocculation albumen) and filtrate,
(4) by step (3) gained filter cake (flocculation albumen) and water mixing homogenizing；
(5) step (4) gained mixed liquor is further dried, obtains Caulis et Folium Brassicae capitatae egg albumen powder.
Wherein, the heating-up temperature described in step (2) is 70-120 DEG C.
Wherein, the drying means described in step (5) includes being spray-dried or lyophilization.
Test data shows, the Caulis et Folium Brassicae capitatae egg albumen powder that said method prepares has the effect of cholesterol in extraordinary reduction blood fat,
Therefore can apply in the fields such as food, health product and medicine.
Detailed description of the invention
By the following examples the present invention is further illustrated, but should not be construed as limitation of the present invention.
Determining the protein quantity method reference: the mensuration of national food safety standard Protein in Food, GB 5009.5-2010.
Embodiment 1
Whole for 20kg strain Caulis et Folium Brassicae capitatae is cleaned, dries, smashed with pulverizer, then squeeze and obtain by filtered through gauze (300 mesh)
Squeezing juice 9.8L and filtering residue, squeezing juice puts into and is heated to 70 DEG C in pot, and albumen flocculation floats over upper strata, to lower floor's redgreen, mistake
Filter to obtain filter cake (flocculation albumen) and filtrate (9.0L).After 9.0L filtrate temperature is down to room temperature, use NF membrane (DK1812C,
GE, the U.S.) it is concentrated to give 2.8L concentrated solution.Will flocculation albumen and concentrated solution mixing and homogenizing, then by be spray-dried (BUCHI,
Small spraying drying instrument B-290) obtain 720g Caulis et Folium Brassicae capitatae egg albumen powder A, protein content is 41%.
Embodiment 2
Whole for 20kg strain Caulis et Folium Brassicae capitatae is cleaned, dries, smashed with pulverizer, then squeeze and obtain by filtered through gauze (300 mesh)
Squeezing juice 10.2L, squeezing juice puts into and is heated to 120 DEG C in pot, and albumen flocculation floats over upper strata, to lower floor's redgreen, filters
Filter cake (flocculation albumen) and filtrate (9.2L).After 9.2L filtrate temperature is down to room temperature, use reverse osmosis membrane (SG1812C, GE,
The U.S.) it is concentrated to give 2.9L concentrated solution.Will flocculation albumen and concentrated solution mixing and homogenizing, then by be spray-dried (BUCHI, little
Type is spray-dried instrument B-290) obtain 718g Caulis et Folium Brassicae capitatae egg albumen powder B, protein content is 38%.
Embodiment 3
Whole for 20kg strain Caulis et Folium Brassicae capitatae is cleaned, dries, smashed with pulverizer, then squeeze and obtain by filtered through gauze (300 mesh)
Squeezing juice 9.5L, squeezing juice puts into and is heated to 70 DEG C in pot, and albumen flocculation floats over upper strata, to lower floor's redgreen, filters and to obtain filter
Cake (flocculation albumen) and filtrate.Flocculation albumen add 2.8L purified water mixing and homogenizing, then by be spray-dried (BUCHI, little
Type is spray-dried instrument B-290) obtain 682g Caulis et Folium Brassicae capitatae egg albumen powder C, protein content is 52%.
Embodiment 4
Cholesterol reducing test drug is the Caulis et Folium Brassicae capitatae egg albumen powder A-C obtained in embodiment 1-3, and experimental animal is Golden Hamster.Test is pressed
Following method is carried out:
1) test method:
Animal adaptability is raised 1 week, and free diet is drunk water, periodicity of illumination 10h/14h, the 8th day, and animal is changed hello high lipid food and (joins
Side: 0.5% cholesterol, 20% Petiolus Trachycarpi oil, 79.5% normal feedstuff), feed 1 week, by animal etherization, after weighing, after socket of the eye
Venous blood collection 0.5ml, anticoagulant heparin, 5000rpm, centrifugal l0min, draw blood plasma, measure in blood plasma with full automatic biochemical apparatus
TC (T-CHOL) and LDL-C (low-density lipoprotein cholesterol) level, choose plasma TC value 9～15mmol/L modeling successful
Proper animal (modeling method sees document Burrier, R. Ε., Smith, Α. Α., CN 101993403B description 22/23
Page；Mcgregor,D.G,Hoos,L.Μ.,Zilli,D.L.,Davis,H.R.The effect of acyl CoA:
cholesterol acyltransferase inhibition on theuptake,esterification and secretion of
cholesterol by the hamster small intestine.J.Pharm.Exp.Ther.,1995,272,156-163)
It is grouped according to TC, LDL_C value and the weight of animals, 8/group, by 10ml/kg volume gastric infusion, is administered 2 weeks, will be dynamic
Thing etherization, after weighing, vena orbitalis posterior blood sampling 0.5ml, anticoagulant heparin, 5000rpm is centrifuged l0min, draws blood plasma, uses
Full automatic biochemical apparatus measures TC and LDL-C level in blood plasma, evaluates sample drug effect.All experiments result is usedRepresent, between group two
Two compare employing t-inspection.P < 0.05 has statistical significance.
2) result of the test:
As shown in table 1, compare with model group, Caulis et Folium Brassicae capitatae egg albumen powder A, B and C all can significantly reduce TC and LDL-C (*P ＜ 0.05,**P ＜ 0.01,***P ＜ 0.001).Compare with model group, * * P ＜ 0.01
The impact on hypercholesterolemia model Golden Hamster blood lipid level of the table 1. Caulis et Folium Brassicae capitatae egg albumen powder repeatedly oral administration
| Group | Dosage (g.kg-1) | TC | LDL-C |
| Model | - | 12.16±1.81 | 1.04±0.21 |
| Caulis et Folium Brassicae capitatae egg albumen powder A | 0.5 | 9.18±0.48*** | 0.71±0.12** |
| Caulis et Folium Brassicae capitatae egg albumen powder B | 0.5 | 9.94±1.30** | 0.78±0.21** |
| Caulis et Folium Brassicae capitatae egg albumen powder C | 0.5 | 8.57±0.78*** | 0.73±0.06*** |
Pharmacodynamics test data show, Caulis et Folium Brassicae capitatae egg albumen powder has in certain reduction blood fat on Golden Hamster hypercholesterolemia model
The effect of cholesterol.
Embodiment 5
Filtration gained filtering residue process after whole strain Caulis et Folium Brassicae capitatae pulverizing in embodiment 1 is dried (Medical Equipment Plant of Shanghai Boxun Industrial Co., Ltd.,
Electric heating constant-temperature blowing drying box GZX-9140 MBE type) after obtain Caulis et Folium Brassicae capitatae dry stem and leaf powder, its protein content is 22%.(in feedstuff
Crude protein determining method, GB/T 6432-1994).
Claims (10)
1. the method preparing Caulis et Folium Brassicae capitatae egg albumen powder, it is characterised in that described method includes:
(1) it is prepared as juice with at least one organ in stem, leaf and three kinds of organs of bouquet of Caulis et Folium Brassicae capitatae for raw material,
(2) protein denaturation in step (1) gained juice is made to flocculate by heating,
(3) step (2) gained mixed liquor is filtered, obtains filter cake and filtrate,
(4) optional, step (3) gained filtrate is cooled to less than 50 DEG C,
(5) step (3) or (4) gained filtrate are carried out membrance concentration process, obtain concentrated solution,
(6) by step (3) gained filter cake and step (5) gained concentrated solution mixing homogenizing,
(7) step (6) gained mixed liquor is further dried, obtains Caulis et Folium Brassicae capitatae egg albumen powder.
Method the most according to claim 1, it is characterised in that the heating-up temperature described in step (2) is 70-120 DEG C.
Method the most according to claim 1 and 2, it is characterised in that the film described in step (5) is selected from reverse osmosis membrane
Or NF membrane, preferably reverse osmosis membrane.
Method the most according to claim 3, it is characterised in that the molecular cut off scope of described NF membrane is 100-500
D, preferably 100-300D.
5. according to the method described in any one of claim 1-4, it is characterised in that the drying means bag described in step (7)
Include spray drying or lyophilization.
6. the Caulis et Folium Brassicae capitatae egg albumen powder prepared according to method described in any one of claim 1-5 is in preparation has reduction blood fat
Purposes in the food of cholesterol or medicine or health product.
7. the preparation method of a Caulis et Folium Brassicae capitatae egg albumen powder, it is characterised in that described method includes:
(1) it is prepared as juice with at least one organ in stem, leaf and three kinds of organs of bouquet of Caulis et Folium Brassicae capitatae for raw material,
(2) protein denaturation in step (1) gained juice is made to flocculate by heating,
(3) step (2) gained mixed liquor is filtered, obtains filter cake and filtrate,
(4) by step (3) gained filter cake and water mixing homogenizing；
(5) step (4) gained mixed liquor is further dried, obtains Caulis et Folium Brassicae capitatae egg albumen powder.
Method the most according to claim 7, it is characterised in that the heating-up temperature described in step (2) is 70-120 DEG C.
9. according to the method described in claim 7 or 8, it is characterised in that the drying means described in step (5) includes spray
Mist is dried or lyophilization.
10. the Caulis et Folium Brassicae capitatae egg albumen powder prepared according to method described in any one of claim 7-9 has reduction blood fat in preparation
Purposes in the food of middle cholesterol or medicine or health product.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107348486A (en) * | 2017-07-19 | 2017-11-17 | 郝之奎 | A kind of manufacture method for the Fermentation Function beverage for preventing and treating cardiovascular and cerebrovascular disease |
| CN107373225A (en) * | 2017-07-19 | 2017-11-24 | 郝之奎 | A kind of broccoli protein peptide drinks and preparation method thereof |
| CN107495389A (en) * | 2017-07-19 | 2017-12-22 | 郝之奎 | A kind of compound protein powder and preparation method thereof |
| CN113134025A (en) * | 2021-04-19 | 2021-07-20 | 台州科技职业学院 | Application of broccoli extract in preventing and treating leukopenia |
| CN115227733A (en) * | 2022-07-22 | 2022-10-25 | 台州科技职业学院 | Application of broccoli extract in preventing and treating leukopenia |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101993480A (en) * | 2009-08-12 | 2011-03-30 | 中国农业科学院饲料研究所 | Broccoli leaf protein and preparation method thereof |
-
2015
- 2015-04-10 CN CN201510167335.7A patent/CN106135630A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101993480A (en) * | 2009-08-12 | 2011-03-30 | 中国农业科学院饲料研究所 | Broccoli leaf protein and preparation method thereof |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107348486A (en) * | 2017-07-19 | 2017-11-17 | 郝之奎 | A kind of manufacture method for the Fermentation Function beverage for preventing and treating cardiovascular and cerebrovascular disease |
| CN107373225A (en) * | 2017-07-19 | 2017-11-24 | 郝之奎 | A kind of broccoli protein peptide drinks and preparation method thereof |
| CN107495389A (en) * | 2017-07-19 | 2017-12-22 | 郝之奎 | A kind of compound protein powder and preparation method thereof |
| CN113134025A (en) * | 2021-04-19 | 2021-07-20 | 台州科技职业学院 | Application of broccoli extract in preventing and treating leukopenia |
| CN115227733A (en) * | 2022-07-22 | 2022-10-25 | 台州科技职业学院 | Application of broccoli extract in preventing and treating leukopenia |
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