CN101984803A - Specimen preserving, fixing and accelerating agent - Google Patents
Specimen preserving, fixing and accelerating agent Download PDFInfo
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- CN101984803A CN101984803A CN2010102919116A CN201010291911A CN101984803A CN 101984803 A CN101984803 A CN 101984803A CN 2010102919116 A CN2010102919116 A CN 2010102919116A CN 201010291911 A CN201010291911 A CN 201010291911A CN 101984803 A CN101984803 A CN 101984803A
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Abstract
The invention discloses a specimen preserving, fixing and accelerating agent which comprises the following components in percentage by weight: 2-20% of main additive, 0.2-5% of auxiliary additive and the balance of ethanol solution of which the concentration is 50vt%, wherein the main additive is selected from laurocapram, thiazone, menthol or isopropyl myristate; and the auxiliary additive is selected from disodium ethylene diamine tetraacetate, ethylene diamine tetraacetic acid tetrasodium salt, ethylene diamine tetraacetic acid dipotassium salt, ethylene diamine tetraacetic acid tripotassium salt, ethylene diamine tetraacetic acid calcium disodium salt or ethylene diamine tetraacetic acid zinc disodium salt. The specimen preserving, fixing and accelerating agent can effectively improve the velocity of permeability of an environment-friendly non-formalin fixing solution in human bodies, animals and plant tissues, shorten the fixing time, and improve the efficiency and the quality for manufacturing pathological sections by a pathology department.
Description
Technical field
The present invention relates to the aseptic technic field, relate in particular to a kind of specimen anti-corrosion fixation accelerator.
Background technology
Formalin (formalin, the formaldehyde dilute solution) is the most frequently used human body, animal, plant tissue specimens preservative, formaldehyde in the formalin can combine with the amino of protein, protein generation irreversible denaturation, it is used for anticorrosion existing more than the 100 year history of human body, animal, plant tissue specimens.At hospital pathology department, Surgical Operating Room, formalin is used for the fixing of pathological tissue sample, and to prevent tissue automatic soup-dissolving, structural deterioration, cytomorphosis, tissue specimen is fixed with fixer after exsomatizing, and makes pathological section, carries out the medical pathologies diagnosis.Medical pathology diagnosis needs high-quality pathological section, and correct sample fixedly is the first step of making high quality slices.
Formalin has high toxicity, carcinogenicity, its toxicity is 30 times of methyl alcohol, long-term contact formalin may cause that chronic respiratory disease, cancer take place, as nasopharyngeal carcinoma, colon cancer, brain tumor, leukemia, cause memory and intelligence to descend, gastric-intestinal perforation, liver damage even stupor can occur when serious; The personnel of long-term contact formalin, the probability that the male sex gets cancer mortality is higher 6.8 times than noncontact personnel, and the women is then high 9 times.
The non-formalin fixed liquid of environment-friendly type of formalin has appearred substituting in recent years comprehensively, it as the patent No. disclosed non-formal Malin fixer in the patent of invention of (ZL200710024048.6), it is anticorrosion, fixing to can be used for human body, animal, plant tissue specimens, product is nontoxic, avoided the occupation infringement of formalin to place staff such as sample preservation, dissection, pathological diagnosis, be subjected to the attention of association area, product is used rapidly in pathology educational circles.The non-formalin fixed liquid of environment-friendly type adopts the natural subsidence fixed mechanism, and the seepage velocity in tissue specimen is lower than formalin, causes the set time longer than formalin, and being extensive use of to hospital pathology department makes troubles.
Summary of the invention
The present invention is intended to overcome the deficiency that above prior art exists, a kind of specimen anti-corrosion fixation accelerator is provided, be used with the non-formalin fixed liquid of environment-friendly type, can effectively improve the seepage velocity of the non-formalin fixed liquid of environment-friendly type in human body, animal, plant tissue, shorten the set time, improve pathology department and make pathological section efficient and quality.
Specimen anti-corrosion fixation accelerator of the present invention comprises each component of following percentage by weight:
Main additive 2-20%
Auxiliary additive 0.2-5%
Surplus is concentration 50vt% (vt: concentration expressed in percentage by volume) ethanolic solution down together;
Main additive mainly acts on the bilayer of cell, increases the flowability of bilayer, lowers phase transition temperature, improves local permeability, promotes the intercellular infiltration of fixed substance; Auxiliary additive can form hydrogen bond fast in albumen after entering tissue, accelerate albuminous degeneration speed.Main additive, auxiliary additive acting in conjunction can obviously improve the seepage velocity of fixer in tissue specimen, shorten the set time.
Described main additive is selected from Laurocapram, thiophene ketone, menthol or myristic acid isopropyl ester;
Described auxiliary additive is selected from disodium ethylene diamine tetraacetate, tetrasodium salt of EDTA, EDTA Dipotassium salt, ethylenediamine tetra-acetic acid tripotassium salt, calcium disodium chelate salt or ethylenediamine tetraacetic acid disodium zinc salt salt.
Further, each component of the preferred following percentage by weight of described specimen anti-corrosion fixation accelerator:
Main additive 2-12%
Auxiliary additive 0.2-5%
Surplus is the ethanolic solution of concentration 50vt%.
Further, each component of the preferred following percentage by weight of described specimen anti-corrosion fixation accelerator:
Main additive 2-12%
Auxiliary additive 0.5-2%
Surplus is the ethanolic solution of concentration 50vt%.
Beneficial effect of the present invention is: this specimen anti-corrosion fixation accelerator has improved the non-formalin fixed liquid of environment-friendly type effectively at the tissue sample, and the seepage velocity in zoological specimens, the plant tissue shortens the set time.Use specimen anti-corrosion fixation accelerator of the present invention, in the tissue sample is fixing, the fixed speed (seepage velocity) of the non-formalin fixed liquid of environment-friendly type is the highest to improve 30%, has reduced the fixing used time, simultaneously SABC antigen is not produced obvious influence.
Specimen anti-corrosion fixation accelerator using method of the present invention: in the non-formalin fixed liquid of environment-friendly type, add 5% volume specimen anti-corrosion fixation accelerator, mix, mixed solution, this mixed solution can carry out the fixing of human body, animal, plant tissue specimens.It is 20 days that this mixed solution uses the term of validity.
Embodiment
Embodiment 1
The percentage by weight of specimen anti-corrosion fixation accelerator consists of Laurocapram 2%, disodium ethylene diamine tetraacetate (EDTA-2Na) 0.2%, and all the other are the ethanolic solution of concentration 50vt%.The tissue specimen fixation test, not adding sample fixation accelerator fixed speed is 5mm/10 hour, adds that fixed speed is 5.6mm/10 hour behind the sample fixation accelerator, fixed speed improves 12%.
Embodiment 2
The percentage by weight of specimen anti-corrosion fixation accelerator consists of Laurocapram 10, disodium ethylene diamine tetraacetate (EDTA-2Na) 5%, and all the other are the ethanolic solution of concentration 50vt%.The tissue specimen fixation test, not adding sample fixation accelerator fixed speed is 5mm/10 hour, adds that fixed speed is 6.5mm/10 hour behind the sample fixation accelerator, fixed speed improves 30%.
Embodiment 3
The percentage by weight of specimen anti-corrosion fixation accelerator consists of thiophene ketone 5%, tetrasodium salt of EDTA (EDTA-4Na) 0.2%, and all the other are the ethanolic solution of concentration 50vt%.The tissue specimen fixation test, not adding sample fixation accelerator fixed speed is 5mm/10 hour, adds that fixed speed is 5.7mm/10 hour behind the sample fixation accelerator, fixed speed improves 14%.
Embodiment 4
The percentage by weight of specimen anti-corrosion fixation accelerator consists of thiophene ketone 10 tetrasodium salt of EDTAs (EDTA-4Na) 3, and all the other are the ethanolic solution of concentration 50vt%.The tissue specimen fixation test, not adding sample fixation accelerator fixed speed is 5mm/10 hour, adds that fixed speed is 6.3mm/10 hour behind the sample fixation accelerator, fixed speed improves 26%.
Embodiment 5
The percentage by weight of specimen anti-corrosion fixation accelerator consists of menthol 15 EDTA Dipotassium salts (EDTA-2K) 0.2%, and all the other are the ethanolic solution of concentration 50vt%.The tissue specimen fixation test, not adding sample fixation accelerator fixed speed is 5mm/10 hour, adds that fixed speed is 5.4mm/10 hour behind the sample fixation accelerator, fixed speed improves 8%.
Embodiment 6
The percentage by weight of specimen anti-corrosion fixation accelerator consists of menthol 20%, EDTA Dipotassium salt (EDTA-2K) 5%, and all the other are the ethanolic solution of concentration 50vt%.The tissue specimen fixation test, not adding sample fixation accelerator fixed speed is 5mm/10 hour, adds that fixed speed is 6mm/10 hour behind the sample fixation accelerator, fixed speed improves 20%.
Embodiment 7
The percentage by weight of specimen anti-corrosion fixation accelerator consists of myristic acid isopropyl ester 2%, ethylenediamine tetra-acetic acid tripotassium salt (EDTA-3K) 0.2%, and all the other are the ethanolic solution of concentration 50vt%.The tissue specimen fixation test, not adding sample fixation accelerator fixed speed is 5mm/10 hour, adds that fixed speed is 5.8mm/10 hour behind the sample fixation accelerator, fixed speed improves 16%.
Embodiment 8
The percentage by weight of specimen anti-corrosion fixation accelerator consists of myristic acid isopropyl ester 20%, ethylenediamine tetra-acetic acid tripotassium salt (EDTA-3K) 3%, and all the other are the ethanolic solution of concentration 50vt%.The tissue specimen fixation test, not adding sample fixation accelerator fixed speed is 5mm/10 hour, adds that fixed speed is 6.1mm/10 hour behind the sample fixation accelerator, fixed speed improves 22%.
Embodiment 9
The percentage by weight of specimen anti-corrosion fixation accelerator consists of Laurocapram 2%, calcium disodium chelate salt (EDTA-2NaCa) 0.2%, and all the other are the ethanolic solution of concentration 50vt%.The tissue specimen fixation test, not adding sample fixation accelerator fixed speed is 5mm/10 hour, adds that fixed speed is 5.7mm/10 hour behind the sample fixation accelerator, fixed speed improves 14%.
Embodiment 10
The percentage by weight of specimen anti-corrosion fixation accelerator consists of thiophene ketone 2%, calcium disodium chelate salt (EDTA-2NaCa) 5%, and all the other are the ethanolic solution of concentration 50vt%.The tissue specimen fixation test, not adding sample fixation accelerator fixed speed is 5mm/10 hour, adds that fixed speed is 5.5mm/10 hour behind the sample fixation accelerator, fixed speed improves 10%.
Embodiment 11
The percentage by weight of specimen anti-corrosion fixation accelerator consists of menthol 20%, ethylenediamine tetraacetic acid disodium zinc salt salt (EDTA-2NaZn) 0.2%, and all the other are the ethanolic solution of concentration 50vt%.The tissue specimen fixation test, not adding sample fixation accelerator fixed speed is 5mm/10 hour, adds that fixed speed is 5.9mm/10 hour behind the sample fixation accelerator, fixed speed improves 18%.
Embodiment 12
The percentage by weight of specimen anti-corrosion fixation accelerator consists of myristic acid isopropyl ester 2%, ethylenediamine tetraacetic acid disodium zinc salt salt (EDTA-2NaZn) 5%, and all the other are the ethanolic solution of concentration 50vt%.The tissue specimen fixation test, not adding sample fixation accelerator fixed speed is 5mm/10 hour, adds that fixed speed is 5.6mm/10 hour behind the sample fixation accelerator, fixed speed improves 12%.
Claims (3)
1. the specimen anti-corrosion fixation accelerator is characterized in that, comprises each component of following percentage by weight:
Main additive 2-20%
Auxiliary additive 0.2-5%
Surplus is the ethanolic solution of concentration 50vt%;
Described main additive is selected from Laurocapram, thiophene ketone, menthol or myristic acid isopropyl ester;
Described auxiliary additive is selected from disodium ethylene diamine tetraacetate, tetrasodium salt of EDTA, EDTA Dipotassium salt, ethylenediamine tetra-acetic acid tripotassium salt, calcium disodium chelate salt or ethylenediamine tetraacetic acid disodium zinc salt salt.
2. specimen anti-corrosion fixation accelerator according to claim 1 is characterized in that, comprises each component of following percentage by weight:
Main additive 2-12%
Auxiliary additive 0.2-5%
Surplus is the ethanolic solution of concentration 50vt%.
3. specimen anti-corrosion fixation accelerator according to claim 1 and 2 is characterized in that: each component that comprises following percentage by weight:
Main additive 2-12%
Auxiliary additive 0.5-2%
Surplus is the ethanolic solution of concentration 50vt%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN 201010291911 CN101984803B (en) | 2010-09-26 | 2010-09-26 | Specimen preserving, fixing and accelerating agent |
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| CN 201010291911 CN101984803B (en) | 2010-09-26 | 2010-09-26 | Specimen preserving, fixing and accelerating agent |
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| CN101984803A true CN101984803A (en) | 2011-03-16 |
| CN101984803B CN101984803B (en) | 2013-01-30 |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103430935A (en) * | 2013-08-27 | 2013-12-11 | 孙志超 | Softening agent for treating butterfly specimen |
| CN105432661A (en) * | 2015-07-08 | 2016-03-30 | 清华大学 | Waste drosophila treatment liquid |
| CN106689120A (en) * | 2017-03-24 | 2017-05-24 | 南阳医学高等专科学校 | Preserving stationary liquid for pathological tissues |
| CN106818713A (en) * | 2017-03-29 | 2017-06-13 | 桂林医学院 | A kind of guarantor color impregnating fluid of pathological tissue specimen |
| CN109377846A (en) * | 2018-10-11 | 2019-02-22 | 内蒙古科技大学包头医学院 | The production method of human anatomy internal organ sample |
| CN111060374A (en) * | 2019-12-31 | 2020-04-24 | 宁夏大学 | GN aldehyde-free rapid fixing liquid and preparation method thereof |
| CN113519505A (en) * | 2021-09-01 | 2021-10-22 | 宁波西敦医药包衣科技有限公司 | Environment-friendly water-based cross-linking agent biological tissue preservation material and preparation method thereof |
| CN113545339A (en) * | 2021-07-12 | 2021-10-26 | 中国林业科学研究院亚热带林业研究所 | Preparation method of bamboo shoot soaked specimen |
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| CN101084746A (en) * | 2007-07-12 | 2007-12-12 | 江苏省原子医学研究所 | Specimen anti-corrosion stationary liquid |
| JP2009107937A (en) * | 2007-10-26 | 2009-05-21 | Sokei:Kk | Gelatinous agent for maintenance and stabilization of corpse |
| CN101473812A (en) * | 2009-01-24 | 2009-07-08 | 曹建明 | Antiseptic fixing liquid composition for cadaver sample and preparation method thereof |
| WO2010001048A2 (en) * | 2008-07-01 | 2010-01-07 | Arkema France | Topical compositions for the preservation of a human or animal body |
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2010
- 2010-09-26 CN CN 201010291911 patent/CN101984803B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101084746A (en) * | 2007-07-12 | 2007-12-12 | 江苏省原子医学研究所 | Specimen anti-corrosion stationary liquid |
| JP2009107937A (en) * | 2007-10-26 | 2009-05-21 | Sokei:Kk | Gelatinous agent for maintenance and stabilization of corpse |
| WO2010001048A2 (en) * | 2008-07-01 | 2010-01-07 | Arkema France | Topical compositions for the preservation of a human or animal body |
| CN101473812A (en) * | 2009-01-24 | 2009-07-08 | 曹建明 | Antiseptic fixing liquid composition for cadaver sample and preparation method thereof |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103430935A (en) * | 2013-08-27 | 2013-12-11 | 孙志超 | Softening agent for treating butterfly specimen |
| CN103430935B (en) * | 2013-08-27 | 2015-05-27 | 孙志超 | Softening agent for treating butterfly specimen |
| CN105432661A (en) * | 2015-07-08 | 2016-03-30 | 清华大学 | Waste drosophila treatment liquid |
| CN106689120A (en) * | 2017-03-24 | 2017-05-24 | 南阳医学高等专科学校 | Preserving stationary liquid for pathological tissues |
| CN106818713A (en) * | 2017-03-29 | 2017-06-13 | 桂林医学院 | A kind of guarantor color impregnating fluid of pathological tissue specimen |
| CN109377846A (en) * | 2018-10-11 | 2019-02-22 | 内蒙古科技大学包头医学院 | The production method of human anatomy internal organ sample |
| CN111060374A (en) * | 2019-12-31 | 2020-04-24 | 宁夏大学 | GN aldehyde-free rapid fixing liquid and preparation method thereof |
| CN113545339A (en) * | 2021-07-12 | 2021-10-26 | 中国林业科学研究院亚热带林业研究所 | Preparation method of bamboo shoot soaked specimen |
| CN113545339B (en) * | 2021-07-12 | 2022-05-20 | 中国林业科学研究院亚热带林业研究所 | Preparation method of bamboo shoot soaked specimen |
| CN113519505A (en) * | 2021-09-01 | 2021-10-22 | 宁波西敦医药包衣科技有限公司 | Environment-friendly water-based cross-linking agent biological tissue preservation material and preparation method thereof |
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| CN101984803B (en) | 2013-01-30 |
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Address after: 214063 Jiangsu city of Wuxi province Qian Rong Lu No. 20 Patentee after: Wuxi Jiangyuan industrial technology and Trade Co.,Ltd. Patentee after: Jiangsu Institute of Nuclear Medicine Address before: 214063 Jiangsu city of Wuxi province Qian Rong Lu No. 20 Patentee before: WUXI JIANGYUAN INDUSTRIAL TECHNOLOGY AND TRADE Corp. Patentee before: Jiangsu Institute of Nuclear Medicine |