CN102246742B - Biological tissue fixing agent and preparation method thereof - Google Patents
Biological tissue fixing agent and preparation method thereof Download PDFInfo
- Publication number
- CN102246742B CN102246742B CN 201110136515 CN201110136515A CN102246742B CN 102246742 B CN102246742 B CN 102246742B CN 201110136515 CN201110136515 CN 201110136515 CN 201110136515 A CN201110136515 A CN 201110136515A CN 102246742 B CN102246742 B CN 102246742B
- Authority
- CN
- China
- Prior art keywords
- fixing agent
- biological tissue
- agar
- water
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a biological tissue fixing agent and a preparation method thereof. The biological tissue fixing agent comprises the following components in percentage by weight: 1-10% of formaldehyde, 1-10% of agar, 0.5-5% of glycerol, 0.2-1% of NaH2PO4, 0.5-2% of Na2HPO4 and the balance of water. The biological tissue fixing agent disclosed by the invention is gelatinous, overcomes the defects of a liquid fixing agent, is convenient in carrying and long-distance mailing, can not leak in a non-tight container, can not cause insufficient fixation resulting from tissue floatation or adherence or the like when tissues are put in the biological tissue fixing agent, is used for fixing needle biopsy tissues, can keep perfect tissue structures and clear cellular morphology, and has a good fixing effect. In the invention, the preparation method is simple, the cost is low, and the period of validity lasts more than one year.
Description
Technical field
The present invention relates to a kind of fixative composition of preserving tissue and biological sample and preparation method thereof.
Background technology
Tissue specimen is fixed with fixative after exsomatizing, and makes pathological section, carries out the medical pathologies diagnosis.The most frequently used fixative is formalin in hospital pathology department, Surgical Operating Room, and formalin is used for the fixing of pathological tissue specimen, to prevent tissue automatic soup-dissolving, structural deterioration, cell degeneration.But formalin is in a liquid state, be inconvenient to carry and long-range mailing, and in not airtight container easy seepage, tissue is put in and cause fixing insufficient such as floating or adherent wherein also can occur organizing.
Summary of the invention
In view of this, the object of the present invention is to provide a kind of biological tissue fixing agent and preparation method thereof, the preparation of this fixative is simple, with low cost, and good fixing effect overcomes the deficiency of liquid fixative, is easy to carry about with one, can seepage.
Biological tissue fixing agent of the present invention, this fixative contain 1%～10% formaldehyde and 1%～10% agar by weight percentage.
Further, this fixative also contains 0.5%～5% glycerine by weight percentage;
Further, this fixative also contains buffer solution;
Further, described buffer solution is NaH
2PO
4, Na
2HPO
4The phosphate buffer that is mixed with water;
Further, the component of this fixative and percentage by weight thereof are:
Formaldehyde 1%～10%;
Agar 1%～10%;
Glycerine 0.5%～5%;
NaH
2PO
4 0.2%～1%；
Na
2HPO
4 0.5%～2%；
Surplus is water.
The preparation method of biological tissue fixing agent of the present invention is: 10%～40% of the total amount of at first fetching water is heated to boiling, adds agar and does not stop to stir, until fully dissolving, then stopped heating replenishes residue water, adds all the other components, stirs to fully cooling.
Therefore can be combined with the amino of protein because of formaldehyde, make protein coagulating, when pharmaceutically can be used as check, organize fixative and preservative etc.; Among the present invention, formaldehyde is used for the fixing of pathological tissue specimen, to prevent tissue automatic soup-dissolving, structural deterioration, cell degeneration; When preparing biological tissue fixing agent of the present invention, the form that formaldehyde can formalin (mass fraction is the formalin of 30-40%) adds.
Agar, the formal name used at school agar-agar, English name agar has another name called agar, agar, delicacy made from bird's nests essence, agel-agal, cold day, large dish slice, be by agar or river hedge (belonging to red algae) through be heated to dissolve after, the marine alga elite that forms of cooled and solidified in addition; The agar made from algae is translucent, unbodied powder, thin slice or particle, and it has coagulability, and stability can form complex compound with some materials; Agar can be used as thickener, coagulating agent, suspending agent, emulsifier, antistaling agent and stabilizing agent, is widely used in manufacturing orange granule juice and various beverage, jelly, ice cream, cake, soft sweets, can, meat products, mixed congee, white fungus bird's nest, custard based food, stir-fried food etc.; Agar can be made medium, ointment base and other purposes in chemical industry, medical research.Among the present invention, agar adds in the fixative as coagulating agent, makes fixative be gel, is convenient for carrying and long-range mailing, also seepage can not occur in not airtight container, and tissue is put in and cause fixing insufficient such as floating or adherent wherein can not occur organizing.
Glycerine, chemistry glycerine by name, pleasantly sweet, be the water white transparency viscous liquid, be the product of Chemical Manufacture, good water imbibition is arranged; The preferred scheme of the present invention owing to glycerine has moisturizing and powerful moisture capacity, thereby can provide lasting moisture for add glycerine in fixative, can be not dry and astringent so that organize, and fixing more abundant.
Buffer system plays an important role to keeping biological normal ph and normal physiological environment, and most cells only can carry out activity in very narrow pH scope, and the pH that need to have buffer system to resist to occur in metabolic process changes.The preferred scheme of the present invention is for adding buffer solution in fixative, to keep within the specific limits relatively stable of pH value, make biological tissue fixing agent of the present invention be more suitable for doing the SABC inspection.Described buffer solution is preferably phosphate buffer, because phosphate buffer is closely neutral, just the strongest at physiological pH scope cushion effect, and cost is relatively low, becoming most widely used, the most universal mineral salt buffer solution of life science, also is one of buffer solution commonly used of immunology, Celluar and Molecular Biology laboratory indispensability.
Beneficial effect of the present invention is:
1, biological tissue fixing agent of the present invention is gel, overcome the deficiency of liquid fixative, be convenient for carrying and long-range mailing, also seepage can not occur in not airtight container, tissue is put in and cause fixing insufficient such as floating or adherent wherein can not occur organizing;
2, the present invention is used for the fixing of aspiration biopsy tissue, and institutional framework is intact, and cellular morphology is clear, good fixing effect;
3, add buffer solution, make fixative be neutral, fixing tissue antigen destroys less, more is conducive to the later stage immunohistochemical staining;
4, preparation method of the present invention is simple, and is with low cost;
5, the fixative term of validity of the present invention is long, through experimental verification, is valid up to more than 1 year.
Embodiment
Below will be described in detail the preferred embodiments of the present invention.
Embodiment 1
10 g that at first fetch water are heated to boiling, add 1g agar and do not stop to stir, until fully dissolving, then stopped heating replenishes 83.8 g water, and the adding mass fraction is 40% formalin 2.5g, NaH
2PO
40.2g, Na
2HPO
42g and glycerine 0.5g stir to fully cooling, make the biological tissue fixing agent of present embodiment.
Embodiment 2
The 20g that at first fetches water is heated to boiling, adds 3g agar and does not stop to stir, until fully dissolving, then stopped heating replenishes 51g water, and the adding mass fraction is 35% formalin 20g, NaH
2PO
40.5g, Na
2HPO
41.5g reach glycerine 4g, stir to fully cooling, make the biological tissue fixing agent of present embodiment.
Embodiment 3
The 26g that at first fetches water is heated to boiling, adds 5g agar and does not stop to stir, until fully dissolving, then stopped heating replenishes 54.5g water, and the adding mass fraction is 35% formalin 10g, NaH
2PO
40.5g, Na
2HPO
41g and glycerine 3g stir to fully cooling, make the biological tissue fixing agent of present embodiment.
Embodiment 4
The 30g that at first fetches water is heated to boiling, adds 8g agar and does not stop to stir, until fully dissolving, then stopped heating replenishes 39g water, and the adding mass fraction is 30% formalin 20g, NaH
2PO
40.3g, Na
2HPO
40.7g reach glycerine 2g, stir to fully cooling, make the biological tissue fixing agent of present embodiment.
Embodiment 5
The 30g that at first fetches water is heated to boiling, adds 10g agar and does not stop to stir, until fully dissolving, then stopped heating replenishes 28.5 g water, and the adding mass fraction is 40% formalin 25g, NaH
2PO
41g, Na
2HPO
40.5g reach glycerine 5g, stir to fully cooling, make the biological tissue fixing agent of present embodiment.
Explanation is at last, above embodiment is only unrestricted in order to technical scheme of the present invention to be described, although by invention has been described with reference to the preferred embodiments of the present invention, but those of ordinary skill in the art is to be understood that, can make various changes to it in the form and details, and not depart from the spirit and scope of the present invention that appended claims limits.
Claims (2)
1. biological tissue fixing agent is characterized in that: this fixative contains 1%～10% formaldehyde and 1%～10% agar by weight percentage, and described fixative also contains buffer solution and 0.5%～5% glycerine by weight percentage, and described buffer solution is NaH
2PO
4, Na
2HPO
4The phosphate buffer that is mixed with water;
The component of described fixative and percentage by weight thereof are:
Formaldehyde 1%～10%;
Agar 1%～10%;
Glycerine 0.5%～5%;
NaH
2PO
4 0.2%～1%；
Na
2HPO
4 0.5%～2%；
Surplus is water.
2. the preparation method of biological tissue fixing agent claimed in claim 1, it is characterized in that: 10%～40% of the total amount of at first fetching water is heated to boiling, adds agar and does not stop to stir, until fully dissolving, then stopped heating replenishes residue water, add all the other components, stir to fully cooling.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110136515 CN102246742B (en) | 2011-05-25 | 2011-05-25 | Biological tissue fixing agent and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110136515 CN102246742B (en) | 2011-05-25 | 2011-05-25 | Biological tissue fixing agent and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102246742A CN102246742A (en) | 2011-11-23 |
| CN102246742B true CN102246742B (en) | 2013-03-13 |
Family
ID=44974608
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110136515 Expired - Fee Related CN102246742B (en) | 2011-05-25 | 2011-05-25 | Biological tissue fixing agent and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102246742B (en) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103013437B (en) * | 2012-12-18 | 2015-06-03 | 中国人民解放军第三军医大学第一附属医院 | Interior label stationary liquid for biological specimen preserved through formalin, preparation method and application thereof |
| CN104663646A (en) * | 2013-11-27 | 2015-06-03 | 深圳先进技术研究院 | Embryonic tissue fixative and its preparation method and use |
| CN104663647A (en) * | 2013-11-27 | 2015-06-03 | 深圳先进技术研究院 | Heart tissue fixing agent as well as preparation method and application of heart tissue fixing agent |
| CN104023526B (en) * | 2014-03-24 | 2016-01-13 | 金仕生物科技（常熟）有限公司 | A kind ofly process the method for biomaterial and the biomaterial through the method process |
| CN105248410B (en) * | 2015-11-17 | 2017-10-03 | 广东海洋大学 | A kind of store method of biological sample |
| ES2673443B1 (en) * | 2016-12-21 | 2019-05-03 | Univ Cardenal Herrera Ceu | PROCEDURE FOR THE CONSERVATION OF BIOLOGICAL SAMPLES USED BY KLOTZ AND PRODUCT OBTAINED WITH SUCH PROCEDURE |
| CN108849855B (en) * | 2018-07-19 | 2021-05-11 | 成都清科生物科技有限公司 | Adipose tissue in-vitro preservation solution and preparation method thereof |
| CN109105362B (en) * | 2018-07-19 | 2021-11-16 | 成都清科生物科技有限公司 | In-vitro deciduous tooth preserving fluid and preparation method thereof |
| CN111397997A (en) * | 2020-04-26 | 2020-07-10 | 中烨(山东)检验检测有限公司 | Tissue fixing liquid for fixing fresh tissue sample |
| CN113545337B (en) * | 2021-07-21 | 2022-08-26 | 益诺思生物技术南通有限公司 | Method for sizing a mammalian heart valve |
-
2011
- 2011-05-25 CN CN 201110136515 patent/CN102246742B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN102246742A (en) | 2011-11-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102246742B (en) | Biological tissue fixing agent and preparation method thereof | |
| CN104120160B (en) | Preparation method for chlorella anti-oxidative peptide | |
| CN106332869B (en) | Fat mesenchymal stem cell frozen stock solution and fat mesenchymal stem cell cryopreservation methods | |
| CN104918486A (en) | Cryoprotecting agent, cryoprotecting and cryopreserved compositions, uses thereof, and methods of cryopreservation | |
| CN104622795B (en) | A kind of sequential sustained-release micro-spheres hydrogel hybrid system of multiple cytokine and preparation method thereof | |
| CN107912420A (en) | A kind of cell preservation method, preserve liquid and preserve liquid and preparation method thereof | |
| CN108486042A (en) | A kind of culture solution in vitro fertilization and preparation method thereof | |
| CN103999849B (en) | The antifreeze of the freezing preservation of a kind of mammal testis tissue and freeze-thaw method | |
| CN111771873A (en) | Method for improving quality of thawed pig sperms | |
| CN103386145B (en) | Wound healing dressing containing carrageenan, and preparation method and application of wound healing dressing | |
| CN108684653A (en) | A kind of placenta preserves liquid and preparation method thereof | |
| CN105432598A (en) | Liquid-based cell preservation liquid and preparation method therefor | |
| AU4435100A (en) | Compositions for freezing dog sperm, method of freezing the dog sperm utilizing the compositions and artificial insemination method employing the frozen dog sperm | |
| WO2024067711A1 (en) | Biological simulation material, and preparation method therefor and use thereof | |
| CN105536064A (en) | Composite type soft tissue repair hydrogel and preparation method and use thereof | |
| Magalhaes et al. | Vitrification successfully preserves hepatocyte spheroids | |
| CN111418579A (en) | Preservation method of adipose tissues, preservation solution of adipose tissues and preparation method of preservation solution | |
| CN114788516A (en) | Sheep semen diluent and preparation method thereof | |
| JP2019135983A (en) | Food for inhibiting photoaging of skin | |
| CN108124853A (en) | A kind of store method of mescenchymal stem cell | |
| CN102240416A (en) | Heparinizing method and application thereof | |
| CN111481659A (en) | Collagen type II and hyaluronic acid composite gel liquid, preparation method and application | |
| CN106076193A (en) | Undenatured collagen base biological surfactant with ionic liquid as reaction medium and preparation method thereof | |
| CN110432258A (en) | Cordycepin is used to prepare the application and preparation method of goat sperm freezen protective dilution | |
| CN115011546B (en) | In-vitro three-dimensional culture, induced differentiation and cryopreservation method for fish cells |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130313 Termination date: 20130525 |