CN102246742A - Biological tissue fixing agent and preparation method thereof - Google Patents
Biological tissue fixing agent and preparation method thereof Download PDFInfo
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- CN102246742A CN102246742A CN2011101365150A CN201110136515A CN102246742A CN 102246742 A CN102246742 A CN 102246742A CN 2011101365150 A CN2011101365150 A CN 2011101365150A CN 201110136515 A CN201110136515 A CN 201110136515A CN 102246742 A CN102246742 A CN 102246742A
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- fixative
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Abstract
The invention discloses a biological tissue fixing agent and a preparation method thereof. The biological tissue fixing agent comprises the following components in percentage by weight: 1-10% of formaldehyde, 1-10% of agar, 0.5-5% of glycerol, 0.2-1% of NaH2PO4, 0.5-2% of Na2HPO4 and the balance of water. The biological tissue fixing agent disclosed by the invention is gelatinous, overcomes the defects of a liquid fixing agent, is convenient in carrying and long-distance mailing, can not leak in a non-tight container, can not cause insufficient fixation resulting from tissue floatation or adherence or the like when tissues are put in the biological tissue fixing agent, is used for fixing needle biopsy tissues, can keep perfect tissue structures and clear cellular morphology, and has a good fixing effect. In the invention, the preparation method is simple, the cost is low, and the period of validity lasts more than one year.
Description
Technical field
The present invention relates to a kind of fixative composition of preserving tissue and biological sample and preparation method thereof.
Background technology
Tissue specimen is fixed with fixative after exsomatizing, and makes pathological section, carries out the medical pathologies diagnosis.The most frequently used fixative is a formalin in hospital pathology department, Surgical Operating Room, and formalin is used for the fixing of pathological tissue sample, to prevent tissue automatic soup-dissolving, structural deterioration, cell degeneration.But formalin is in a liquid state, be inconvenient to carry and long-range mailing, and in not airtight container easy seepage, tissue is put in and cause fixing insufficient such as floating or adherent wherein also can occur organizing.
Summary of the invention
In view of this, the object of the present invention is to provide a kind of biological tissue fixative and preparation method thereof, the preparation of this fixative is simple, with low cost, and good fixing effect overcomes the deficiency of liquid fixative, is easy to carry about with one, can seepage.
Biological tissue of the present invention fixative, this fixative contain 1%～10% formaldehyde and 1%～10% agar by weight percentage.
Further, this fixative also contains 0.5%～5% glycerine by weight percentage;
Further, this fixative also contains buffer solution;
Further, described buffer solution is NaH
2PO
4, Na
2HPO
4The phosphate buffer that is mixed with water;
Further, the component of this fixative and percentage by weight thereof are:
Formaldehyde 1%～10%;
Agar 1%～10%;
Glycerine 0.5%～5%;
NaH
2PO
4 0.2%～1%；
Na
2HPO
4 0.5%～2%；
Surplus is a water.
The preparation method of biological tissue of the present invention fixative is: 10%～40% of the total amount of at first fetching water is heated to boiling, adds agar and does not stop to stir, and until dissolving fully, stops heating then, replenishes surplus water, adds all the other components, stirs to cooling fully.
Because of formaldehyde can combine with the amino of protein, make protein coagulating, therefore fixation of tissue agent when pharmaceutically can be used as check and preservative etc.; Among the present invention, formaldehyde is used for the fixing of pathological tissue sample, to prevent tissue automatic soup-dissolving, structural deterioration, cell degeneration; When preparing biological tissue of the present invention fixative, the form that formaldehyde can formalin (mass fraction is the formalin of 30-40%) adds.
Agar, the formal name used at school agar-agar, English name agar has another name called agar, agar, delicacy made from bird's nests essence, agel-agal, cold day, last course of a feast silk, be by agar or river hedge (belonging to red algae) through be heated to dissolve after, the marine alga elite that forms of cooled and solidified in addition; The agar made from algae is translucent, unbodied powder, thin slice or particle, and it has coagulability, and stability can form complex compound with some materials; Agar can be used as thickener, coagulating agent, suspending agent, emulsifier, antistaling agent and stabilizing agent, is widely used in manufacturing orange granule juice and various beverage, jelly, ice cream, cake, soft sweets, can, meat products, mixed congee, white fungus bird's nest, custard based food, stir-fried food or the like; Agar can be made medium, ointment base and other purposes in chemical industry, medical research.Among the present invention, agar adds in the fixative as coagulating agent, makes fixative be gel, is convenient for carrying and long-range mailing, also seepage can not occur in not airtight container, and tissue is put in and cause fixing insufficient such as floating or adherent wherein can not occur organizing.
Glycerine, chemistry glycerine by name, pleasantly sweet, be the water white transparency viscous liquid, be the product of Chemical Manufacture, good water imbibition is arranged; The preferred scheme of the present invention is preserved moisture and powerful moisture capacity owing to glycerine has, thereby lasting moisture can be provided for add glycerine in fixative, and feasible organizing can be not dry and astringent, and is fixing more abundant.
Buffer system plays an important role to keeping biological normal ph and normal physiological environment, and most cells only can carry out activity in very narrow pH scope, and needs buffer system and resist the pH that occurs change in metabolic process.The preferred scheme of the present invention is for adding buffer solution in fixative, to keep within the specific limits relatively stable of pH value, make biological tissue of the present invention fixative be more suitable for doing the SABC inspection.Described buffer solution is preferably phosphate buffer, because phosphate buffer is closely neutral, just the strongest at physiological pH scope cushion effect, and cost is relatively low, becoming most widely used, the most universal mineral salt buffer solution of life science, also is one of buffer solution commonly used of immunology, Celluar and Molecular Biology laboratory indispensability.
Beneficial effect of the present invention is:
1, biological tissue of the present invention fixative is gel, overcome the deficiency of liquid fixative, be convenient for carrying and long-range mailing, also seepage can not occur in not airtight container, tissue is put in and cause fixing insufficient such as floating or adherent wherein can not occur organizing;
2, the present invention is used for the fixing of aspiration biopsy tissue, and institutional framework is intact, and cellular morphology is clear, good fixing effect;
3, add buffer solution, make fixative be neutral, fixing tissue antigen destroys less, more helps the later stage immunohistochemical staining;
4, preparation method of the present invention is simple, and is with low cost;
5, the fixative term of validity of the present invention is long, through experimental verification, is valid up to more than 1 year.
Embodiment
Below will be described in detail the preferred embodiments of the present invention.
Embodiment 1
10 g that at first fetch water are heated to boiling, and adding 1g agar does not stop to stir, and until dissolving fully, stops heating then, replenishes 83.8 g water, and the adding mass fraction is 40% formalin 2.5g, NaH
2PO
40.2g, Na
2HPO
42g and glycerine 0.5g stir to cooling fully, make biological tissue's fixative of present embodiment.
Embodiment 2
The 20g that at first fetches water is heated to boiling, and adding 3g agar does not stop to stir, and until dissolving fully, stops heating then, replenishes 51g water, and the adding mass fraction is 35% formalin 20g, NaH
2PO
40.5g, Na
2HPO
41.5g reach glycerine 4g, stir to cooling fully, make biological tissue's fixative of present embodiment.
Embodiment 3
The 26g that at first fetches water is heated to boiling, and adding 5g agar does not stop to stir, and until dissolving fully, stops heating then, replenishes 54.5g water, and the adding mass fraction is 35% formalin 10g, NaH
2PO
40.5g, Na
2HPO
41g and glycerine 3g stir to cooling fully, make biological tissue's fixative of present embodiment.
Embodiment 4
The 30g that at first fetches water is heated to boiling, and adding 8g agar does not stop to stir, and until dissolving fully, stops heating then, replenishes 39g water, and the adding mass fraction is 30% formalin 20g, NaH
2PO
40.3g, Na
2HPO
40.7g reach glycerine 2g, stir to cooling fully, make biological tissue's fixative of present embodiment.
Embodiment 5
The 30g that at first fetches water is heated to boiling, and adding 10g agar does not stop to stir, and until dissolving fully, stops heating then, replenishes 28.5 g water, and the adding mass fraction is 40% formalin 25g, NaH
2PO
41g, Na
2HPO
40.5g reach glycerine 5g, stir to cooling fully, make biological tissue's fixative of present embodiment.
Explanation is at last, above embodiment is only unrestricted in order to technical scheme of the present invention to be described, although by invention has been described with reference to the preferred embodiments of the present invention, but those of ordinary skill in the art is to be understood that, can make various changes to it in the form and details, and the spirit and scope of the present invention that do not depart from appended claims and limited.
Claims (6)
1. biological tissue's fixative is characterized in that: this fixative contains 1%～10% formaldehyde and 1%～10% agar by weight percentage.
2. biological tissue according to claim 1 fixative is characterized in that: this fixative also contains 0.5%～5% glycerine by weight percentage.
3. biological tissue according to claim 2 fixative is characterized in that: this fixative also contains buffer solution.
4. biological tissue according to claim 3 fixative is characterized in that: described buffer solution is NaH
2PO
4, Na
2HPO
4The phosphate buffer that is mixed with water.
5. biological tissue according to claim 4 fixative is characterized in that: the component of this fixative and percentage by weight thereof are:
Formaldehyde 1%～10%;
Agar 1%～10%;
Glycerine 0.5%～5%;
NaH
2PO
4 0.2%～1%；
Na
2HPO
4 0.5%～2%；
Surplus is a water.
6. the preparation method of any described biological tissue fixative of claim 1 to 5, it is characterized in that: 10%～40% of the total amount of at first fetching water is heated to boiling, adding agar does not stop to stir, until dissolving fully, stop heating then, replenish surplus water, add all the other components, stir to cooling fully.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110136515 CN102246742B (en) | 2011-05-25 | 2011-05-25 | Biological tissue fixing agent and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110136515 CN102246742B (en) | 2011-05-25 | 2011-05-25 | Biological tissue fixing agent and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102246742A true CN102246742A (en) | 2011-11-23 |
| CN102246742B CN102246742B (en) | 2013-03-13 |
Family
ID=44974608
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|---|---|---|---|
| CN 201110136515 Expired - Fee Related CN102246742B (en) | 2011-05-25 | 2011-05-25 | Biological tissue fixing agent and preparation method thereof |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103013437A (en) * | 2012-12-18 | 2013-04-03 | 中国人民解放军第三军医大学第一附属医院 | Interior label stationary liquid for biological specimen preserved through formalin, preparation method and application thereof |
| CN104663647A (en) * | 2013-11-27 | 2015-06-03 | 深圳先进技术研究院 | Heart tissue fixing agent as well as preparation method and application of heart tissue fixing agent |
| CN104663646A (en) * | 2013-11-27 | 2015-06-03 | 深圳先进技术研究院 | Embryonic tissue fixative and its preparation method and use |
| WO2015143611A1 (en) * | 2014-03-24 | 2015-10-01 | 金仕生物科技（常熟）有限公司 | Biological material treating method, and biological material treated by using same |
| CN105248410A (en) * | 2015-11-17 | 2016-01-20 | 广东海洋大学 | Preservation method for biological specimen |
| ES2673443R1 (en) * | 2016-12-21 | 2018-07-04 | Universidad Cardenal Herrera Ceu | PROCEDURE FOR THE CONSERVATION OF BIOLOGICAL SAMPLES USED BY KLOTZ AND PRODUCT OBTAINED WITH SUCH PROCEDURE |
| CN108849855A (en) * | 2018-07-19 | 2018-11-23 | 成都清科生物科技有限公司 | A kind of adipose tissue Plantlet in vitro liquid and preparation method thereof |
| CN109105362A (en) * | 2018-07-19 | 2019-01-01 | 成都清科生物科技有限公司 | A kind of deciduous teeth in vitro saves liquid and preparation method thereof |
| CN111397997A (en) * | 2020-04-26 | 2020-07-10 | 中烨(山东)检验检测有限公司 | Tissue fixing liquid for fixing fresh tissue sample |
| CN113545337A (en) * | 2021-07-21 | 2021-10-26 | 益诺思生物技术南通有限公司 | Method for sizing a mammalian heart valve |
-
2011
- 2011-05-25 CN CN 201110136515 patent/CN102246742B/en not_active Expired - Fee Related
Non-Patent Citations (3)
| Title |
|---|
| 《青岛海洋大学学报》 20010930 赵元莙等 海水养殖中原生动物病原虫的鉴定-II粘孢子虫的研究及鉴定方法 第681-688页 第1-6 第31卷, 第5期 * |
| 赵元莙等: "海水养殖中原生动物病原虫的鉴定—II粘孢子虫的研究及鉴定方法", 《青岛海洋大学学报》 * |
| 陈海滨等: "一种培养细胞切片用于免疫细胞化学染色方法的建立", 《汕头大学医学院学报》 * |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103013437A (en) * | 2012-12-18 | 2013-04-03 | 中国人民解放军第三军医大学第一附属医院 | Interior label stationary liquid for biological specimen preserved through formalin, preparation method and application thereof |
| CN103013437B (en) * | 2012-12-18 | 2015-06-03 | 中国人民解放军第三军医大学第一附属医院 | Interior label stationary liquid for biological specimen preserved through formalin, preparation method and application thereof |
| CN104663647A (en) * | 2013-11-27 | 2015-06-03 | 深圳先进技术研究院 | Heart tissue fixing agent as well as preparation method and application of heart tissue fixing agent |
| CN104663646A (en) * | 2013-11-27 | 2015-06-03 | 深圳先进技术研究院 | Embryonic tissue fixative and its preparation method and use |
| WO2015143611A1 (en) * | 2014-03-24 | 2015-10-01 | 金仕生物科技（常熟）有限公司 | Biological material treating method, and biological material treated by using same |
| CN105248410A (en) * | 2015-11-17 | 2016-01-20 | 广东海洋大学 | Preservation method for biological specimen |
| ES2673443R1 (en) * | 2016-12-21 | 2018-07-04 | Universidad Cardenal Herrera Ceu | PROCEDURE FOR THE CONSERVATION OF BIOLOGICAL SAMPLES USED BY KLOTZ AND PRODUCT OBTAINED WITH SUCH PROCEDURE |
| CN108849855A (en) * | 2018-07-19 | 2018-11-23 | 成都清科生物科技有限公司 | A kind of adipose tissue Plantlet in vitro liquid and preparation method thereof |
| CN109105362A (en) * | 2018-07-19 | 2019-01-01 | 成都清科生物科技有限公司 | A kind of deciduous teeth in vitro saves liquid and preparation method thereof |
| CN108849855B (en) * | 2018-07-19 | 2021-05-11 | 成都清科生物科技有限公司 | Adipose tissue in-vitro preservation solution and preparation method thereof |
| CN109105362B (en) * | 2018-07-19 | 2021-11-16 | 成都清科生物科技有限公司 | In-vitro deciduous tooth preserving fluid and preparation method thereof |
| CN111397997A (en) * | 2020-04-26 | 2020-07-10 | 中烨(山东)检验检测有限公司 | Tissue fixing liquid for fixing fresh tissue sample |
| CN113545337A (en) * | 2021-07-21 | 2021-10-26 | 益诺思生物技术南通有限公司 | Method for sizing a mammalian heart valve |
| CN113545337B (en) * | 2021-07-21 | 2022-08-26 | 益诺思生物技术南通有限公司 | Method for sizing a mammalian heart valve |
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|---|---|
| CN102246742B (en) | 2013-03-13 |
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Granted publication date: 20130313 Termination date: 20130525 |