CN101979632A - Method for producing theaflavin by fermenting by adopting phase transfer enzyme catalysis technology - Google Patents
Method for producing theaflavin by fermenting by adopting phase transfer enzyme catalysis technology Download PDFInfo
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- CN101979632A CN101979632A CN2010105206888A CN201010520688A CN101979632A CN 101979632 A CN101979632 A CN 101979632A CN 2010105206888 A CN2010105206888 A CN 2010105206888A CN 201010520688 A CN201010520688 A CN 201010520688A CN 101979632 A CN101979632 A CN 101979632A
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- theoflavin
- phase transition
- ethyl acetate
- reduced pressure
- secondary response
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Abstract
The invention relates to a method for extracting tea polyphenol by adopting phase transfer technology. The method is characterized by comprising the following steps of: cutting and crushing fresh tea leaves serving as an enzyme source; adding dry tea leaves, a phase transfer agent and an extracting agent serving as raw materials for extracting the tea polyphenol; blasting air or oxygen while stirring at room temperature; separating fermentation liquor; repeatedly adding the extracting agent for performing secondary fermentation and thrice fermentation; merging the fermentation liquor; concentrating under a reduced pressure until the fermentation liquor is nearly dry; adding water into concentrated solution; continuing concentrating under the reduced pressure and desolventizing to obtain the aqueous solution of theaflavin; centrifuging and filtering precipitated chlorophyll; and performing spray-drying, vacuum-drying or freeze-drying on the aqueous solution of the theaflavin from which the chlorophyll is removed to obtain solid theaflavin. In the method, the process is simple; all raw materials are natural green agricultural and sideline products; the process saves energy and environmental-friendly; a process production does not produce process spent water; equipment investment is simple; and production cost is low.
Description
Technical field
The present invention relates to a kind of method that adopts phase transition enzyme technology fermentative production theoflavin, belong to technical field of food additives.
Background technology
At present, tea-polyphenol has become large plant milk extract kind, is raw material with it, adopts the vitro enzyme enzymatic oxidation to produce theoflavin, is the special type of oxidation that utilizes polyphenoloxidase POP, and the selective paraffin oxidation tea-polyphenol is produced the method for theoflavin.Various active such as that theoflavin has is anticancer, resisting cardiovascular disease, inhibitory anti-virus have wide application prospect in natural drug, functional foodstuff and functional beverage field.Publication number is the technology of CN1626668A, adopts Fresh Folium Camelliae sinensis to smash slagging and juice to pieces, behind the enzyme that adding market is bought, is added in the mixing solutions of tea-polyphenol and organic solvent, adds aqueous acid again, stirs the reaction of ventilating, enzymatic fermentative production theoflavin.But this technology is added the finished product tea-polyphenol as fermentation raw material, and cost is higher, and fermented liquid contains the easy emulsification of a large amount of water capacity, separation difficulty.Publication number is the technology of CN1403580A, adopts polyphenoloxidase is carried out immobilization with embedded material, under the certain reaction condition, makes highly purified tea-polyphenol oxypolymerization become highly purified theoflavin.The enzyme immobilization embedding program trivial operations of this technology, cost is higher, and the purge process of enzyme can cause certain environmental pollution.
Summary of the invention
The objective of the invention is to overcome above-mentioned weak point, directly adopting the dried tea dust that extracts tea-polyphenol is raw material, utilize the natural polyphenol oxydase in the Fresh Folium Camelliae sinensis, directly prepare theoflavin with phase transition enzyme catalysis fermentation technique, provide a kind of less energy-consumption low pollution, the method of production theoflavin easy and simple to handle, production process does not produce process spent water, and facility investment simple production cost is low.
In order to achieve the above object, the invention provides a kind of method that adopts phase transition enzyme technology fermentative production theoflavin, it is characterized in that concrete steps are:
The first step: Fresh Folium Camelliae sinensis stirred to cut with the rotor kneading and cutting machine be ground into the Fresh Folium Camelliae sinensis slag as the natural enzyme powder, standby;
Second step: get raw material tealeaves 1 weight part, add natural enzyme powder 0.7～7.0 weight part and ethyl acetate 10～15 weight parts of polyoxyethylene glycol or glycerin solution 1 ~ 2 weight part, the first step preparation, obtain phase transition enzymatic system;
The 3rd step: under the agitation condition, blast air or oxygen in the second phase transition enzymatic system that obtain of step, aeration-agitation is 2 ~ 3 hours under the room temperature, obtain the theoflavin reaction solution, filter out 1 secondary response liquid, add the ethyl acetate of 5 ~ 10 weight parts again in filter residue, aeration-agitation is 2 hours under the room temperature, obtain 2 secondary response liquid, separating and filtering goes out 2 secondary response liquid, adds 5 ~ 10 weight part ethyl acetate in filter residue again, and aeration-agitation is 2 hours under the room temperature, obtain 3 secondary response liquid, separating and filtering goes out 3 secondary response liquid;
The 4th step: merge above-mentioned 1-3 secondary response liquid, concentrating under reduced pressure obtains concentrated solution, adds the water of 1 ~ 2 weight part, removes ethyl acetate under reduced pressure, the chlorophyll that suction filtration or centrifugal filtering are separated out;
The 5th step: will remove chlorophyllous theoflavin aqueous solution concentrating under reduced pressure drying or spraying drying in the 4th step, and get Powdered theoflavin product.
Fresh Folium Camelliae sinensis in the described the first step is preferably the bright leaf of natural large leaf tea.
Consisting of phase-transferring agent in described second step is preferably the polyoxyethylene glycol aqueous solution or glycerin solution.
The raw material tealeaves in described second step is scrap stock or the old tea of green tea that produces in the green tea process for processing.
The invention has the beneficial effects as follows: technology of the present invention is simple, energy consumption is low, pollution-free, the dried tea dust that tea-polyphenol is produced in employing is a raw material, Fresh Folium Camelliae sinensis is as the enzyme source, tea-polyphenol in the dried tea dust of one-step catalytic generates theoflavin, direct production cost can be reduced, the environmental protection treatment cost pressure can be reduced again.The present invention is intended to form a kind of phase transition enzyme catalysis system, after being raw material tea dust and natural enzyme powder absorption consisting of phase-transferring agent, form solid phase-water-ester micro-system mutually at raw material tealeaves with natural enzyme powder surface, tea-polyphenol by the solid phase diffusion of raw material tealeaves to water, diffuse to again ester mutually in, diffuse to the aqueous phase on natural enzyme powder surface then, by aqueous phase dissolved polyphenoloxidase is that catalyzed polymerization generates theoflavin, theoflavin is extracted mutually by ester again, and having avoided the enzyme in the enzyme powder is to be poisoned by reaction product to lose katalysis.Present method gained theoflavin purity is more than 40%, and residue is based on unoxidized tea-polyphenol, and product can be in food-drink merchant widespread use, or carries out further refiningly, obtains being used for the theoflavin product of medicine.The yield of present method theoflavin is 10% ~ 15% of a stem tea weight, and in the polyphenol content in the tealeaves, the catalysis yield is up to more than 50%.
Embodiment
Specify the present invention below in conjunction with embodiment.
Embodiment 1
Get 2 kilograms on the bright leaf of large leaf green tea, stir with the rotor kneading and cutting machine and to cut pulverizing, the 10%(W/W that adds 2 kilograms at dried green tea end, polyoxyethylene glycol successively) 2 kilograms of the aqueous solution, 20 kilograms of extraction agent ethyl acetate are at room temperature roused air conditions and are mixed stirring 3 hours down, filter out one time fermentation liquid with silk.Add 16 kilograms of ethyl acetate again, under room temperature drum air conditions, mix and stirred 2 hours, filter out Secondary Fermentation liquid with silk.Add 15 kilograms of ethyl acetate again, at room temperature rouse air conditions and mix stirring 2 hours down, filter out fermented liquid three times with silk.Merge fermented liquid, concentrating under reduced pressure is closely dried, adds 2 kg of water, removes ethyl acetate under reduced pressure, the centrifugal chlorophyll of separating out of removing.Remove chlorophyllous theoflavin solution for vacuum drying, drying conditions is 50 ℃, and vacuum tightness obtains 206 gram powdery products, theoflavin content 41.2% greater than-0.06MPa.
Embodiment 2
Get 2 kilograms on the bright leaf of large leaf green tea, stir with the rotor kneading and cutting machine and to cut pulverizing, the 30%(W/W that adds 2.5 kilograms at dried green tea end, glycerol successively) 3 kilograms of the aqueous solution, 20 kilograms of extraction agent ethyl acetate are at room temperature roused air conditions and are mixed stirring 3 hours down, filter out one time fermentation liquid with silk.Add 20 kilograms of ethyl acetate again, under room temperature drum air conditions, mix and stirred 2 hours, filter out Secondary Fermentation liquid with silk.Add 18 kilograms of ethyl acetate again, at room temperature rouse air conditions and mix stirring 2 hours down, filter out fermented liquid three times with silk.Merge fermented liquid, concentrating under reduced pressure is closely dried, adds 3 kg of water, removes ethyl acetate under reduced pressure, the centrifugal chlorophyll of separating out of removing.Remove chlorophyllous theoflavin solution for vacuum drying, drying conditions is 50 ℃, and vacuum tightness obtains 262 gram powdery products, theoflavin content 41.8% greater than-0.06MPa.
Embodiment 3
Get 3 kilograms on the bright leaf of large leaf green tea, stir with the rotor kneading and cutting machine and to cut pulverizing, the 10%(W/W that adds 2.5 kilograms at dried green tea end, polyoxyethylene glycol successively) 1 kilogram of the aqueous solution, glycerol 20%(W/W) 1.5 kilograms of the aqueous solution, 25 kilograms of extraction agent ethyl acetate, at room temperature rouse air conditions and mix stirring 3 hours down, filter out one time fermentation liquid with silk.Add 25 kilograms of ethyl acetate again, under room temperature drum air conditions, mix and stirred 2 hours, filter out Secondary Fermentation liquid with silk.Add 20 kilograms of ethyl acetate again, at room temperature rouse air conditions and mix stirring 2 hours down, filter out fermented liquid three times with silk.Merge fermented liquid, concentrating under reduced pressure is closely dried, adds 2.5 kg of water, removes ethyl acetate under reduced pressure, the centrifugal chlorophyll of separating out of removing.Remove chlorophyllous theoflavin solution for vacuum drying, drying conditions is 50 ℃, and vacuum tightness obtains 285 gram powdery products, theoflavin content 42.5% greater than-0.06MPa.
Claims (4)
1. method that adopts phase transition enzyme technology fermentative production theoflavin is characterized in that concrete steps are:
The first step: Fresh Folium Camelliae sinensis stirred to cut with the rotor kneading and cutting machine be ground into the Fresh Folium Camelliae sinensis slag as the natural enzyme powder, standby;
Second step: get raw material tealeaves 1 weight part, add natural enzyme powder 0.7～7.0 weight part and ethyl acetate 10～15 weight parts of consisting of phase-transferring agent 1 ~ 2 weight part, the first step preparation, obtain phase transition enzymatic system;
The 3rd step: under the agitation condition, blast air or oxygen in the second phase transition enzymatic system that obtain of step, aeration-agitation is 2 ~ 3 hours under the room temperature, obtain the theoflavin reaction solution, filter out 1 secondary response liquid, add the ethyl acetate of 5 ~ 10 weight parts again in filter residue, aeration-agitation is 2 hours under the room temperature, obtain 2 secondary response liquid, separating and filtering goes out 2 secondary response liquid, adds 5 ~ 10 weight part ethyl acetate in filter residue again, and aeration-agitation is 2 hours under the room temperature, obtain 3 secondary response liquid, separating and filtering goes out 3 secondary response liquid;
The 4th step: merge above-mentioned 1-3 secondary response liquid, concentrating under reduced pressure obtains concentrated solution, adds the water of 1 ~ 2 weight part, removes ethyl acetate under reduced pressure, the chlorophyll that suction filtration or centrifugal filtering are separated out;
The 5th step: will remove chlorophyllous theoflavin aqueous solution concentrating under reduced pressure drying or spraying drying in the 4th step, and get Powdered theoflavin product.
2. the method for employing phase transition enzyme technology fermentative production theoflavin as claimed in claim 1 is characterized in that, the Fresh Folium Camelliae sinensis in the described the first step is the bright leaf of natural large leaf tea.
3. the method for employing phase transition enzyme technology fermentative production theoflavin as claimed in claim 1 is characterized in that, the consisting of phase-transferring agent in described second step is the polyoxyethylene glycol aqueous solution or glycerin solution.
4. the method for employing phase transition enzyme technology fermentative production theoflavin as claimed in claim 1 is characterized in that, the raw material tealeaves in described second step is scrap stock or the old tea of green tea that produces in the green tea process for processing.
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| CN2010105206888A CN101979632A (en) | 2010-10-27 | 2010-10-27 | Method for producing theaflavin by fermenting by adopting phase transfer enzyme catalysis technology |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102286565A (en) * | 2011-06-10 | 2011-12-21 | 安徽大学 | Preparation method of theaflavin monomer |
| CN104450822A (en) * | 2014-12-19 | 2015-03-25 | 雅安太时生物科技有限公司 | Method for producing theaflavin from tea polyphenol production wastewater |
| CN108285914A (en) * | 2017-12-03 | 2018-07-17 | 新疆阜丰生物科技有限公司 | A kind of zymotechnique of L-Trp |
| CN110652007A (en) * | 2019-11-06 | 2020-01-07 | 满山歌茶业(西双版纳)有限公司 | Method for preparing theaflavin |
| CN111621531A (en) * | 2020-06-01 | 2020-09-04 | 浙江天草生物科技股份有限公司 | Method for producing theaflavin by utilizing branches and leaves of Anji white tea pruning |
| CN112553265A (en) * | 2020-11-23 | 2021-03-26 | 南通德诺生物技术有限公司 | Method for preparing theaflavin by enzyme catalysis and product prepared by method |
| CN114807264A (en) * | 2021-01-18 | 2022-07-29 | 南通德诺生物技术有限公司 | Method for preparing theaflavin by using exogenous enzyme and product prepared by method |
-
2010
- 2010-10-27 CN CN2010105206888A patent/CN101979632A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102286565A (en) * | 2011-06-10 | 2011-12-21 | 安徽大学 | Preparation method of theaflavin monomer |
| CN104450822A (en) * | 2014-12-19 | 2015-03-25 | 雅安太时生物科技有限公司 | Method for producing theaflavin from tea polyphenol production wastewater |
| CN108285914A (en) * | 2017-12-03 | 2018-07-17 | 新疆阜丰生物科技有限公司 | A kind of zymotechnique of L-Trp |
| CN110652007A (en) * | 2019-11-06 | 2020-01-07 | 满山歌茶业(西双版纳)有限公司 | Method for preparing theaflavin |
| CN111621531A (en) * | 2020-06-01 | 2020-09-04 | 浙江天草生物科技股份有限公司 | Method for producing theaflavin by utilizing branches and leaves of Anji white tea pruning |
| CN112553265A (en) * | 2020-11-23 | 2021-03-26 | 南通德诺生物技术有限公司 | Method for preparing theaflavin by enzyme catalysis and product prepared by method |
| CN114807264A (en) * | 2021-01-18 | 2022-07-29 | 南通德诺生物技术有限公司 | Method for preparing theaflavin by using exogenous enzyme and product prepared by method |
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Application publication date: 20110223 |