CN113797233A - Novel traditional Chinese medicine decoction piece processing technology based on biological enzymolysis technology - Google Patents
Novel traditional Chinese medicine decoction piece processing technology based on biological enzymolysis technology Download PDFInfo
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- CN113797233A CN113797233A CN202111284415.2A CN202111284415A CN113797233A CN 113797233 A CN113797233 A CN 113797233A CN 202111284415 A CN202111284415 A CN 202111284415A CN 113797233 A CN113797233 A CN 113797233A
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- enzymolysis
- chinese medicine
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- liquid separation
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
- A61K9/1605—Excipients; Inactive ingredients
- A61K9/1629—Organic macromolecular compounds
- A61K9/1652—Polysaccharides, e.g. alginate, cellulose derivatives; Cyclodextrin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
- A61K9/1605—Excipients; Inactive ingredients
- A61K9/1664—Compounds of unknown constitution, e.g. material from plants or animals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
Abstract
The invention relates to a novel processing technology of traditional Chinese medicine decoction pieces by a basic biological enzymolysis technology. The method specifically comprises the following steps: firstly, breaking the wall of the Chinese herbal pieces, crushing, performing enzymolysis by complex enzyme, filtering, performing secondary extraction, concentrating, drying, adding auxiliary materials, and granulating. The wall-broken powder is subjected to mild extraction by biological enzymolysis, so that the effective active ingredients are greatly improved, the fluidity and the instant solubility of the wall-broken powder are improved after the wall-broken powder is prepared into granules, the wall-broken powder can be directly taken with water without decoction, and the time and the labor are saved.
Description
Technical Field
The invention relates to the technical field of deep processing of traditional Chinese medicine decoction pieces, in particular to a novel traditional Chinese medicine decoction piece processing technology based on a biological enzymolysis technology.
Background
The traditional Chinese medicine decoction pieces are generally prepared by slicing, cutting into sections, drying in the sun, drying, frying or steaming, and the like, most of the traditional Chinese medicine decoction pieces are decocted, but the decoction time of the traditional Chinese medicine is long, time and labor are wasted, and the traditional Chinese medicine decoction pieces are difficult to operate by people in cities with fast life rhythm; in recent years, the traditional Chinese medicine wall-broken decoction pieces have been widely researched in the field of traditional Chinese medicine due to the advantages of full-ingredient retention, high ingredient utilization rate, convenient use and the like.
The obtaining and releasing of the active ingredients of the traditional Chinese medicine are carried out through a molecular movement process that the traditional Chinese medicine particles absorb water and swell, and medicinal chemical ingredients migrate (diffuse) from the inside of cells to an extracellular solvent, wherein the former process is completed in vitro, and the latter process is completed in vivo.
Disclosure of Invention
According to the defects in the prior art, the invention provides a novel traditional Chinese medicine decoction piece processing technology based on a biological enzymolysis technology for solving the problems.
The technical scheme adopted by the invention is as follows:
a novel traditional Chinese medicine decoction piece processing technology based on a biological enzymolysis technology comprises the following steps:
firstly, carrying out superfine grinding on traditional Chinese medicine decoction pieces, adding complex enzyme for enzymolysis, carrying out solid-liquid separation, carrying out secondary extraction on wet residues after solid-liquid separation, carrying out secondary solid-liquid separation, concentrating and drying, then adding auxiliary materials for granulation, and carrying out split charging and packaging to obtain the novel traditional Chinese medicine decoction pieces. The compound enzyme is any five or more than five of acid cellulase, acid pectinase, alkaline protease, acid protease, papain, lipase, amylase, glucanase and pentosanase.
The specific operation steps are as follows:
firstly, carrying out superfine grinding on traditional Chinese medicine decoction pieces.
Then the crushed raw materials are put into water with a set proportion and mixed evenly, and complex enzyme is added for enzymolysis.
After the enzymolysis is finished, performing solid-liquid separation, and collecting enzymolysis liquid for later use
Transferring the wet slag to a reflux device, supplementing water to the original volume, and refluxing and extracting for 2 hours by boiling water.
And performing secondary solid-liquid separation, and collecting the leaching liquor.
And combining the supernatants obtained in the steps 10 and 12, transferring the combined supernatants into a distillation device, and concentrating the combined supernatants to 1/2 of the original volume under vacuum and reduced pressure.
And (4) carrying out spray drying on the concentrated supernatant to obtain dry powder.
Adding beta-cyclodextrin, maltodextrin and honey into the dry powder according to a certain proportion, uniformly mixing, and putting into a granulator for granulation.
The traditional Chinese medicine decoction pieces are crushed to the granularity of 1000-1500 meshes.
Preferably, the particle-to-water ratio is 1:15 to 1: 30.
The preferable composite enzymolysis time is 2-3 hours.
The pH value of the preferred compound enzyme is 4.6-6.0
The preferable adding amount of the compound enzyme is 1.5-3% (based on the dry weight of the medicinal powder).
Preferably, the compound enzyme is composed of the following components in percentage by weight: 10-25% of acidic cellulase, 10-15% of acidic pectinase, 0-10% of alkaline protease, 1-6% of acidic protease, 0-2% of papain, 0-5% of lipase, 2-8% of amylase, 0-1% of glucanase and 0-3% of pentosanase.
Further preferably, the enzymatic activities of the components in the complex enzyme are as follows: 50 ten thousand u/g of acid cellulase, 30 ten thousand u/g of acid pectinase, 10 ten thousand u/g of alkaline protease, 20 ten thousand u/g of acid protease, 20 ten thousand u/g of papain, 50 ten thousand u/g of lipase, 20 ten thousand u/g of amylase, 20 ten thousand u/g of glucanase and 1000 ten thousand u/g of pentosanase.
The preferable solid-liquid separation is performed at the rotating speed of 5000r/min and the centrifugation is performed for 30 min.
The preferred vacuum decompression temperature is 70 ℃.
The preferable spray drying process is to control the temperature to be 200 ℃, the sample feeding speed of a peristaltic pump to be 14rpm, the air inlet temperature to be 96 ℃, and the air outlet temperature to be 100 ℃.
Preferably, the mixture is granulated and added with 15 to 20 percent of beta-cyclodextrin, 20 to 30 percent of malt dextrin and 40 to 60 percent of honey.
Through a large number of experimental verifications and analyses, the invention selects proper enzyme preparation and addition amount for enzymolysis, obviously improves the product yield and reduces the degradation of effective active ingredients.
The method has the advantages of simple and safe technical process, easy operation, suitability for industrial scale enlargement and improvement of economic benefits of deep processing of the traditional Chinese medicine decoction pieces.
Detailed Description
The technical solutions in the embodiments of the present invention are described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
1. Micronizing decoction pieces of Chinese medicinal materials, breaking cell wall for 20 min, and sieving with 1500 mesh sieve to obtain cell wall-broken fine powder.
2. Adding distilled water into the crushed raw materials according to the material-water ratio of 1:20, and fully and uniformly stirring.
3. Adding complex enzyme 2.5% (calculated on dry raw materials), keeping the temperature at 55 ℃ for enzymolysis for 2 hours, wherein the enzyme comprises the following components in parts by mass: 15% of acid cellulase, 10% of acid pectinase, 2% of acid protease, 1% of lipase and 2% of amylase.
4. And (4) carrying out solid-liquid separation on the enzymolysis liquid after enzymolysis, and centrifuging for 25min at 5000 r/min.
5. The obtained wet residue is transferred into a reflux device, and the reflux extraction time of boiling water is 2 hours.
6. And carrying out secondary solid-liquid separation on the secondary leaching solution, and combining the secondary leaching solution with the enzymolysis solution.
7. Transferring the extractive solution into a concentrating device, and vacuum concentrating under reduced pressure to 1/2.
8. Spray drying the concentrated solution to obtain powder.
9. Adding 15% of beta-cyclodextrin, 20% of maltodextrin and 40% of honey into the dry powder for granulating.
10. The extraction rate of soluble substances in terms of dry raw material substances finally reaches 87.96 percent through the extraction of the steps
Example 2
1. Micronizing decoction pieces of Chinese medicinal materials, breaking cell wall for 20 min, and sieving with 1500 mesh sieve to obtain cell wall-broken fine powder.
2. Adding distilled water into the crushed raw materials according to the material-water ratio of 1:20, and fully and uniformly stirring.
3. Adding 3.0% of complex enzyme (calculated by dry raw materials), keeping the temperature at 55 ℃ for enzymolysis for 2 hours, wherein the enzyme comprises the following components in parts by mass: 10% of acid cellulase, 12% of acid pectinase, 3% of acid protease, 2% of lipase and 3% of amylase.
4. And (4) carrying out solid-liquid separation on the enzymolysis liquid after enzymolysis, and centrifuging for 25min at 5000 r/min.
5. The obtained wet residue is transferred into a reflux device, and the reflux extraction time of boiling water is 2 hours.
6. And carrying out secondary solid-liquid separation on the secondary leaching solution, and combining the secondary leaching solution with the enzymolysis solution.
7. Transferring the extractive solution into a concentrating device, and vacuum concentrating under reduced pressure to 1/2.
8. Spray drying the concentrated solution to obtain powder.
9. Adding 15% of beta-cyclodextrin, 25% of maltodextrin and 50% of honey into the dry powder for granulating.
10. The extraction rate of soluble substances in terms of dry raw material substances finally reaches 85.35 percent through the extraction of the steps
Example 3
1. Micronizing decoction pieces of Chinese medicinal materials, breaking cell wall for 20 min, and sieving with 1500 mesh sieve to obtain cell wall-broken fine powder.
2. Adding distilled water into the crushed raw materials according to the material-water ratio of 1:20, and fully and uniformly stirring.
3. Adding 1.0% of complex enzyme (calculated by dry raw materials), keeping the temperature at 55 ℃ for enzymolysis for 2 hours, wherein the enzyme comprises the following components in parts by mass: 20% of acid cellulase, 10% of acid pectinase, 5% of acid protease, 5% of lipase and 5% of amylase.
4. And (4) carrying out solid-liquid separation on the enzymolysis liquid after enzymolysis, and centrifuging for 25min at 5000 r/min.
5. The obtained wet residue is transferred into a reflux device, and the reflux extraction time of boiling water is 2 hours.
6. And carrying out secondary solid-liquid separation on the secondary leaching solution, and combining the secondary leaching solution with the enzymolysis solution.
7. Transferring the extractive solution into a concentrating device, and vacuum concentrating under reduced pressure to 1/2.
8. Spray drying the concentrated solution to obtain powder.
9. Adding 20% of beta-cyclodextrin, 30% of maltodextrin and 55% of honey into the dry powder for granulation.
10. The soluble extraction rate of the final dry raw material by the extraction of the steps reaches 82.33 percent
Example 4
1, taking the traditional Chinese medicine decoction pieces, carrying out superfine grinding, breaking the wall for 20 minutes, and sieving with a 1500-mesh sieve to obtain wall-broken fine powder.
2. Adding distilled water into the crushed raw materials according to the material-water ratio of 1:20, and fully and uniformly stirring.
3. Adding 1.5% of complex enzyme (calculated by dry raw materials), keeping the temperature at 55 ℃ for enzymolysis for 2 hours, wherein the enzyme comprises the following components in parts by mass: 25% of acid cellulase, 10% of acid pectinase, 5% of acid protease, 3% of lipase and 6% of amylase.
4. And (4) carrying out solid-liquid separation on the enzymolysis liquid after enzymolysis, and centrifuging for 25min at 5000 r/min.
5. The obtained wet residue is transferred into a reflux device, and the reflux extraction time of boiling water is 2 hours.
6. And carrying out secondary solid-liquid separation on the secondary leaching solution, and combining the secondary leaching solution with the enzymolysis solution.
7. Transferring the extractive solution into a concentrating device, and vacuum concentrating under reduced pressure to 1/2.
8. Spray drying the concentrated solution to obtain powder.
9. Adding 15% of beta-cyclodextrin, 30% of malt dextrin and 60% of honey into the dry powder for granulation.
10. The extraction rate of soluble substances calculated by dry raw material substances finally reaches 80.46 percent through the extraction of the steps.
Claims (9)
1. The patent provides a novel traditional Chinese medicine decoction piece processing technology based on a biological enzymolysis technology, which comprises the following steps: firstly, carrying out superfine grinding on the raw materials, adding complex enzyme for enzymolysis, carrying out solid-liquid separation, carrying out secondary extraction on wet residues after the solid-liquid separation, carrying out secondary solid-liquid separation, concentrating and drying, then adding auxiliary materials for granulation, and carrying out split charging and packaging to obtain the novel traditional Chinese medicine decoction pieces.
2. The method of claim 1, wherein the decoction pieces are subjected to micronization, wall breaking for 15-30 min, and 1500 mesh sieving to obtain wall-broken fine powder.
3. The method of claim 1, wherein the complex enzyme is any five or more of acid cellulase, acid pectinase, alkaline protease, acid protease, papain, lipase, amylase, glucanase, and pentosanase.
4. The method of claim 1, wherein the complex enzyme is composed of the following components in percentage by weight: 10-25% of acidic cellulase, 10-15% of acidic pectinase, 0-10% of alkaline protease, 1-6% of acidic protease, 0-2% of papain, 0-5% of lipase, 2-8% of amylase, 0-1% of glucanase and 0-3% of pentosanase.
5. The method of claim 1, wherein the enzymolysis temperature is 50-65 ℃ for 2-3 hours, and the enzymolysis pH is 5.5-6.5.
6. The method according to claim 1, wherein the solid-liquid separation is a centrifugation method, wherein the rotation speed is 5000r/min, and the centrifugation is 25-30 min.
7. The method according to claim 1, wherein the wet residue secondary leaching is a boiling water reflux method, and the reflux extraction time is 2 hours.
8. The method of claim 1, wherein the concentrating is followed by spray drying to a powder using a spray drying tower.
9. The method of claim 1, wherein the excipients added for granulation are beta-cyclodextrin, maltodextrin, honey.
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| CN202111284415.2A CN113797233A (en) | 2021-11-02 | 2021-11-02 | Novel traditional Chinese medicine decoction piece processing technology based on biological enzymolysis technology |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115337328A (en) * | 2022-08-31 | 2022-11-15 | 山东省现代中药研究院有限公司 | Preparation method of medicinal and edible traditional Chinese medicine decoction pieces based on biological enzymolysis technology |
| CN116236519A (en) * | 2023-04-27 | 2023-06-09 | 江西普正制药股份有限公司 | Processing method of salvia yunnanensis decoction pieces |
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2021
- 2021-11-02 CN CN202111284415.2A patent/CN113797233A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115337328A (en) * | 2022-08-31 | 2022-11-15 | 山东省现代中药研究院有限公司 | Preparation method of medicinal and edible traditional Chinese medicine decoction pieces based on biological enzymolysis technology |
| CN116236519A (en) * | 2023-04-27 | 2023-06-09 | 江西普正制药股份有限公司 | Processing method of salvia yunnanensis decoction pieces |
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Application publication date: 20211217 |
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