CN101970465B - 能够结合rna帽的流感病毒pb2蛋白可溶性片段 - Google Patents

能够结合rna帽的流感病毒pb2蛋白可溶性片段 Download PDF

Info

Publication number
CN101970465B
CN101970465B CN200880119968.9A CN200880119968A CN101970465B CN 101970465 B CN101970465 B CN 101970465B CN 200880119968 A CN200880119968 A CN 200880119968A CN 101970465 B CN101970465 B CN 101970465B
Authority
CN
China
Prior art keywords
seq
amino acid
amino acids
rna
polypeptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN200880119968.9A
Other languages
English (en)
Chinese (zh)
Other versions
CN101970465A (zh
Inventor
斯蒂芬·库萨克
德尔菲娜·吉利盖
达伦·哈特
弗兰克·塔伦多
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Europaisches Laboratorium fuer Molekularbiologie EMBL
Original Assignee
Europaisches Laboratorium fuer Molekularbiologie EMBL
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Europaisches Laboratorium fuer Molekularbiologie EMBL filed Critical Europaisches Laboratorium fuer Molekularbiologie EMBL
Publication of CN101970465A publication Critical patent/CN101970465A/zh
Application granted granted Critical
Publication of CN101970465B publication Critical patent/CN101970465B/zh
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D1/00—Rigid or semi-rigid containers having bodies formed in one piece, e.g. by casting metallic material, by moulding plastics, by blowing vitreous material, by throwing ceramic material, by moulding pulped fibrous material or by deep-drawing operations performed on sheet material
    • B65D1/02—Bottles or similar containers with necks or like restricted apertures, designed for pouring contents
    • B65D1/0223—Bottles or similar containers with necks or like restricted apertures, designed for pouring contents characterised by shape
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A61P31/16—Antivirals for RNA viruses for influenza or rhinoviruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D2501/00—Containers having bodies formed in one piece
    • B65D2501/0009—Bottles or similar containers with necks or like restricted apertures designed for pouring contents
    • B65D2501/0018—Ribs
    • B65D2501/0036—Hollow circonferential ribs
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2299/00—Coordinates from 3D structures of peptides, e.g. proteins or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
    • C12N2760/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08—RNA viruses
    • G01N2333/11—Orthomyxoviridae, e.g. influenza virus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/04—Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Oncology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Communicable Diseases (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mechanical Engineering (AREA)
  • Ceramic Engineering (AREA)
  • Pulmonology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Containers Having Bodies Formed In One Piece (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
CN200880119968.9A 2007-10-09 2008-10-09 能够结合rna帽的流感病毒pb2蛋白可溶性片段 Expired - Fee Related CN101970465B (zh)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US99839807P 2007-10-09 2007-10-09
US60/998,398 2007-10-09
US7079208P 2008-03-25 2008-03-25
US61/070,792 2008-03-25
US12345608P 2008-04-08 2008-04-08
US61/123,456 2008-04-08
PCT/EP2008/008543 WO2009046983A1 (en) 2007-10-09 2008-10-09 Soluble fragments of influenza virus pb2 protein capable of binding rna-cap

Publications (2)

Publication Number Publication Date
CN101970465A CN101970465A (zh) 2011-02-09
CN101970465B true CN101970465B (zh) 2016-03-16

Family

ID=40364724

Family Applications (1)

Application Number Title Priority Date Filing Date
CN200880119968.9A Expired - Fee Related CN101970465B (zh) 2007-10-09 2008-10-09 能够结合rna帽的流感病毒pb2蛋白可溶性片段

Country Status (11)

Country Link
US (1) US9045248B2 (https=)
EP (2) EP2197901B1 (https=)
JP (3) JP5725861B2 (https=)
KR (1) KR101672554B1 (https=)
CN (1) CN101970465B (https=)
AU (1) AU2008309939B2 (https=)
CA (1) CA2701362A1 (https=)
ES (1) ES2454773T3 (https=)
IL (3) IL204891A (https=)
SG (1) SG185289A1 (https=)
WO (1) WO2009046983A1 (https=)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2197901B1 (en) * 2007-10-09 2013-12-11 European Molecular Biology Laboratory (Embl) Soluble fragments of influenza virus pb2 protein capable of binding rna-cap
LT3141252T (lt) 2009-06-17 2018-11-12 Vertex Pharmaceuticals Inc. Gripo virusų replikacijos inhibitoriai
KR20130075726A (ko) * 2010-03-16 2013-07-05 유럽피안 몰레큘러 바이올로지 래보러토리 유행성 인플루엔자 바이러스 ａ 2009 ｈ1ｎ1로부터의 ｒｎａ 의존성 ｒｎａ 폴리머라제의 ｐａ 아단위의 단편 및 이의 용도
RU2013132681A (ru) 2010-12-16 2015-01-27 Вертекс Фармасьютикалз Инкорпорейтед Ингибиторы репликации вирусов гриппа
UA118010C2 (uk) 2011-08-01 2018-11-12 Вертекс Фармасьютікалз Інкорпорейтед Інгібітори реплікації вірусів грипу
HK1209140A1 (en) * 2012-07-19 2016-03-24 Zoetis Services Llc Bovine influenza c virus compositions
JP6618901B2 (ja) 2013-11-13 2019-12-11 バーテックス ファーマシューティカルズ インコーポレイテッドＶｅｒｔｅｘ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ Ｉｎｃｏｒｐｏｒａｔｅｄ インフルエンザウイルスの複製の阻害剤を調製する方法
LT3068776T (lt) 2013-11-13 2019-08-12 Vertex Pharmaceuticals Incorporated Gripo virusų replikacijos inhibitoriai
CN105018441A (zh) * 2014-04-22 2015-11-04 中国科学院生物物理研究所 流感病毒rna聚合酶结晶的方法
CN105002149B (zh) * 2014-04-22 2018-04-06 中国科学院生物物理研究所 流感病毒rna聚合酶纯化或结晶的方法
EP3294717B1 (en) 2015-05-13 2020-07-29 Vertex Pharmaceuticals Inc. Methods of preparing inhibitors of influenza viruses replication
EP3294735B8 (en) 2015-05-13 2022-01-05 Vertex Pharmaceuticals Incorporated Inhibitors of influenza viruses replication
CN107286044B (zh) 2016-04-13 2021-07-13 香港神农有限公司 一种能够抑制流感病毒pb2蛋白与rna帽结合的化合物
CL2018003871A1 (es) * 2018-12-28 2021-01-15 Univ Pontificia Catolica Chile Anticuerpos monoclonales específicos para el antígeno pb2 del virus de la influenza humana (flu), secuencias nucleotídicas; método y kit de diagnóstico de infección producida por flu
WO2020239822A1 (en) 2019-05-27 2020-12-03 The European Molecular Biology Laboratory Nucleic acid construct binding to influenza polymerase pb1 rna synthesis active site
CN113122596B (zh) * 2020-08-20 2023-01-24 深圳市瑞吉生物科技有限公司 一种Cap2结构5′帽子类似物及其制备方法和应用
CN114741315B (zh) * 2022-04-28 2023-06-13 深圳市紫光同创电子有限公司 用例更新方法、装置、电子设备及存储介质
CN116024301B (zh) * 2023-01-16 2025-10-28 北京协同创新研究院 一种rna依赖性rna聚合酶截短体的筛选方法、截短体及应用
WO2025055169A1 (en) * 2023-09-12 2025-03-20 The Chinese University Of Hong Kong A fluorescent probe to measure rna binding and elongation

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5837852A (en) * 1993-10-14 1998-11-17 Bristol-Myers Squibb Company Capped nucleic acid oligomers that inhibit cap-dependent transcription of the influenza virus endonuclease

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2197901B1 (en) 2007-10-09 2013-12-11 European Molecular Biology Laboratory (Embl) Soluble fragments of influenza virus pb2 protein capable of binding rna-cap
JP6614523B2 (ja) * 2014-03-28 2019-12-04 地方独立行政法人神奈川県立産業技術総合研究所 インフルエンザウイルスのｒｎａ依存性ｒｎａポリメラーゼのｐｂ２サブユニットに対するモノクローナル抗体

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5837852A (en) * 1993-10-14 1998-11-17 Bristol-Myers Squibb Company Capped nucleic acid oligomers that inhibit cap-dependent transcription of the influenza virus endonuclease

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Recoginition of mRNA cap structures by viral and cellular proteins;Pierre Fechter et al;《Journal of General Virology》;20051231;第86卷;1239-1249 *
Two Aromotic Residues in the PB2 Subunit of Influenza A RNA Polymerase Are Crucial for Cap Binding;Pierre Fechter et al;《THE JOURNAL OF BIOLOGICAL CHEMISTRY》;20030315;第278卷(第22期);20381-20388 *
Two separate sequences of PB2 subunit constitute the RNA cap-binding site of influenza virus RNA polymerse;Ayae Honda et al;《Genes to Cells》;19991231;第4卷;475-485 *

Also Published As

Publication number Publication date
IL237903A (en) 2017-10-31
WO2009046983A1 (en) 2009-04-16
SG185289A1 (en) 2012-11-29
IL204891A (en) 2015-04-30
EP2574619A3 (en) 2013-07-31
CA2701362A1 (en) 2009-04-16
JP5937169B2 (ja) 2016-06-22
KR20100074212A (ko) 2010-07-01
JP2011501942A (ja) 2011-01-20
IL222997A0 (en) 2012-12-31
EP2197901A1 (en) 2010-06-23
JP5725861B2 (ja) 2015-05-27
AU2008309939B2 (en) 2013-11-14
IL204891A0 (en) 2010-11-30
JP6309563B2 (ja) 2018-04-11
ES2454773T3 (es) 2014-04-11
US9045248B2 (en) 2015-06-02
AU2008309939A1 (en) 2009-04-16
EP2197901B1 (en) 2013-12-11
EP2574619A2 (en) 2013-04-03
KR101672554B1 (ko) 2016-11-03
CN101970465A (zh) 2011-02-09
JP2016176955A (ja) 2016-10-06
JP2015062417A (ja) 2015-04-09
US20110008348A1 (en) 2011-01-13

Similar Documents

Publication Publication Date Title
CN101970465B (zh) 能够结合rna帽的流感病毒pb2蛋白可溶性片段
US20180010109A1 (en) Polypeptide fragments comprising endonuclease activity and their use
US20180044649A1 (en) Influenza a 2009 pandemic h1n1 polypeptide fragments comprising endonuclease activity and their use
HK1179985A (en) Soluble fragments of influenza virus pb2 protein capable of binding rna-cap
HK1183501A (en) Polypeptide fragments comprising endonuclease activity and their use
HK1163166B (en) Polypeptide fragments comprising endonuclease activity and their use
HK1163166A (en) Polypeptide fragments comprising endonuclease activity and their use

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20160316

Termination date: 20181009