CN101966224A - Method for producing astragalus and codonopsis pilosula compound extract - Google Patents
Method for producing astragalus and codonopsis pilosula compound extract Download PDFInfo
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- CN101966224A CN101966224A CN2010105125363A CN201010512536A CN101966224A CN 101966224 A CN101966224 A CN 101966224A CN 2010105125363 A CN2010105125363 A CN 2010105125363A CN 201010512536 A CN201010512536 A CN 201010512536A CN 101966224 A CN101966224 A CN 101966224A
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- radix astragali
- radix codonopsis
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Abstract
The invention relates to a method for producing an astragalus and codonopsis pilosula compound extract, which comprises the following process steps of: 1, breaking plant cell walls by using enzymolysis technology; 2, changing the pH value of extracting solution, and continuously decocting and extracting; and 3, concentrating under reduced pressure, adsorbing by using macroporous resin, eluting by using ethanol, and performing spray drying to obtain the finished product. The method improves the extraction yield of astragalus and codonopsis pilosula, which is 11.7 percent; and the polysaccharide content in the extract is over 70 percent. The extraction efficiency is 4 times that of the conventional water extraction, and the active ingredients of the astragalus and codonopsis pilosula are fully extracted.
Description
Technical field
The present invention relates to feedstuff and field of feed additive technology, relate in particular to the production method of the Radix Astragali, Radix Codonopsis compound extract.
Background technology
The Radix Astragali and Radix Codonopsis all are traditional conventional Chinese medicines, have the effect of invigorating the spleen and replenishing QI, and the modern pharmacology proof all has the effect of enhancing immunity.The Radix Codonopsis sweet in the mouth is flat, has invigorating the spleen and replenishing QI, spleen invigorating lung benefiting function; Radix Astragali sweet in the mouth is warm in nature, has tonifying Qi and lifting yang, benefit is defended functions such as consolidating superficial resistance, inducing diuresis to remove edema, expelling pus and toxin by strengthening QI, expelling pus and promoting granulation.Radix Codonopsis mainly contains Radix Codonopsis polysaccharide, flavonoid, alkaloid and volatilization wet goods composition, has pharmacological actions such as enhance immunity, defying age, antioxidation, antitumor.The Radix Astragali mainly contains astragalus polysaccharides, it is the main component of Radix Astragali performance immunological enhancement, also have multiple compositions such as astragaloside, flavonoid, aminoacid, trace element in addition, have enhance immunity, antitumor, antiviral, antioxidation, the anti-ageing multiple function of waiting for a long time, can influence the growth of immune organs such as animal thymus, spleen and fabricius bursa, promote lymphocyte transformation and propagation and macrophage phagocytic function, improve the cellular immunization and the humoral immunization ability of animal body.
Utilize the compatibility advantage of the Radix Astragali and two kinds of medicines of Radix Codonopsis and the mutual enhancing and the complementarity of function, the Radix Astragali that extraction is developed, Radix Codonopsis compound extract, can be used as the immunity of organism regulator, improving animal body immunity and controlling animal immune and suppress to have significant effect aspect the disease.
Extraction to polysaccharide effective ingredient in the Radix Astragali and the Radix Codonopsis is the key point of the Radix Astragali, Radix Codonopsis compound extract (Radix Astragali, Radix Codonopsis complex) production technology.Polysaccharide extracting process at present traditional Chinese herbal medicine is a water extract-alcohol precipitation, and polysaccharide extracts yield and is about 2.5%, and it is low to extract yield, and efficient is poor, and its production effect that is applied to the Radix Astragali, Radix Codonopsis compound extract is unsatisfactory.
Summary of the invention
Carry out the above-mentioned defective that ginseng-astragalus polysaccharides extracts existence at the existing water extract-alcohol precipitation mode that adopts, the applicant has carried out research and has improved, the production method of the another kind of Radix Astragali, Radix Codonopsis compound extract is provided, earlier break plant cell wall earlier with zymolysis technique, change the extracting solution pH value again and proceed to decoct extraction, thereby improve extraction ratio.
The technical solution adopted in the present invention is as follows:
The production method of a kind of Radix Astragali, Radix Codonopsis compound extract, processing step is as follows:
(1) Radix Astragali, Radix Codonopsis decoction pieces are soaked, used cellulase degradation 30 minutes, decoct the 30 minutes after-filtration that reflux, obtain filtrate and filtering residue;
(2) will be added the CaO alkalescent water of 8 times of weight by the filtering residue that step (1) obtains, the 30 minutes after-filtration that reflux obtain filtrate and filtering residue;
(3) will be added the sodium acetate aqueous solution of 8 times of weight by the filtering residue that step (2) obtain, the 30 minutes after-filtration that reflux obtain filtrate and filtering residue;
(4) filtrate in combining step (1), step (2) and the step (3) is 1.01 with the filtrate after merging in 60 ℃ of relative densities that are evaporated to for water, uses macroporous resin adsorption impurity;
(5) carry out eluting with 30% ethanol, reclaim ethanol, spray drying obtains finished product.
Its further technical scheme is: the addition of described cellulase is a 0.5kg/ ton water.
Its further technical scheme is: the mass concentration of described CaO alkalescent water is 1%.
Its further technical scheme is: the mass concentration of described sodium acetate aqueous solution is 0.5%.
Its further technical scheme is: the weight proportion of the Radix Astragali, Radix Codonopsis is 4: 1 in the described Radix Astragali, the Radix Codonopsis compound extract.
Useful technique effect of the present invention is:
The Radix Astragali of the present invention, Radix Codonopsis compound extract (Radix Astragali, Radix Codonopsis complex) adopt enzymolysis and semi-bionic extraction method, break plant cell wall earlier with zymolysis technique earlier, change the extracting solution pH value again and proceed to decoct extraction.The solution that conciliation PH uses is respectively calcium oxide solution and sodium acetate solution.Calcium oxide solution is adjusted to pH value about 8.4, and sodium acetate solution is adjusted to pH value about 5.9, simulates the absorbing environmental in the gastrointestinal respectively, has improved the extraction yield, the experiment proved that the raising amount reaches 11.7%, and the polyoses content in the extract reaches more than 70%.Extraction efficiency is 4 times of conventional water extraction, guarantees that the effective ingredient of the Radix Astragali, Radix Codonopsis is fully extracted.
Description of drawings
Fig. 1 is a process chart of the present invention.
The specific embodiment
Below in conjunction with accompanying drawing, the specific embodiment of the present invention is described.
As shown in Figure 1, the step of the production method of the Radix Astragali of the present invention, Radix Codonopsis compound extract is:
The Radix Astragali, Radix Codonopsis decoction pieces (Radix Astragali, Radix Codonopsis weight proportion are 4: 1) are soaked enzymolysis (cellulase, addition are 0.5kg/ ton water) 30 minutes, decoct and refluxed 30 minutes, filter, get filtrate 1 and filtering residue.Filtering residue is added the CaO alkalescent water (mass concentration is 1%) of 4～12 times of weight, refluxed 30 minutes, filter, filtrate 2 and filtering residue.Filtering residue is added the sodium acetate aqueous solution (mass concentration is 0.5%) of 4～12 times of weight, refluxed 30 minutes, filter.Get filtrate 3 and filtering residue.Merging filtrate 1, filtrate 2, filtrate 3, in 60 ℃ be evaporated to respect to water for density 1.01 (60 ℃).Decoction liquor by macroporous resin, is adsorbed effective ingredient wherein.Carry out eluting with 30% ethanol at last, material is transferred to be dissolved in ethanol, thereby taken away by ethanol elution.Adopt the way of distillation that ethanol is recovered in the ethanol storage tank, after the spray drying (inlet temperature 145 degree, leaving air temp 80 degree), get product.
In order better to explain the present invention, the present invention will be further described below in conjunction with the specific embodiment.
Embodiment 1
The 100kg Radix Astragali, Radix Codonopsis decoction pieces (Radix Astragali, Radix Codonopsis weight proportion are 4: 1) are soaked,, are decocted and refluxed 30 minutes, filter with cellulase (addition is a 0.5kg/ ton water) enzymolysis 30 minutes, filtrate 1 and filtering residue.It is 1% CaO alkalescent water that filtering residue is added 4 times of weight mass concentrations, refluxes 30 minutes, filter, filtrate 2 and filtering residue.It is 0.5% sodium acetate aqueous solution that filtering residue is added 4 times of weight mass concentrations, refluxes 30 minutes, filters.Get filtrate 3 and filtering residue.Merging filtrate 1, filtrate 2, filtrate 3 are 1.01 (60 ℃) in 60 ℃ of density that are evaporated to respect to water, use macroporous resin adsorption.Carry out eluting with 30% ethanol at last, reclaim ethanol, spray drying promptly.
Embodiment 2
The 100kg Radix Astragali, Radix Codonopsis decoction pieces (Radix Astragali, Radix Codonopsis weight proportion are 4: 1) are soaked,, are decocted and refluxed 30 minutes, filter with cellulase (addition is a 0.5kg/ ton water) enzymolysis 30 minutes, filtrate 1 and filtering residue.It is 1% CaO alkalescent water that filtering residue is added 8 times of weight mass concentrations, refluxes 30 minutes, filter, filtrate 2 and filtering residue.It is 0.5% sodium acetate aqueous solution that filtering residue is added 8 times of weight mass concentrations, refluxes 30 minutes, filters.Get filtrate 3 and filtering residue.Merging filtrate 1, filtrate 2, filtrate 3 are 1.01 (60 ℃) in 60 ℃ of density that are evaporated to respect to water, use macroporous resin adsorption.Carry out eluting with 30% ethanol at last, reclaim ethanol, spray drying promptly.
Embodiment 3
The 100kg Radix Astragali, Radix Codonopsis decoction pieces (Radix Astragali, Radix Codonopsis weight proportion are 4: 1) are soaked,, are decocted and refluxed 30 minutes, filter with cellulase (addition is a 0.5kg/ ton water) enzymolysis 30 minutes, filtrate 1 and filtering residue.It is 1% CaO alkalescent water that filtering residue is added 12 times of weight mass concentrations, refluxes 30 minutes, filter, filtrate 2 and filtering residue.It is 0.5% sodium acetate aqueous solution that filtering residue is added 12 times of weight mass concentrations, refluxes 30 minutes, filters.Get filtrate 3 and filtering residue.Merging filtrate 1, filtrate 2, filtrate 3 are 1.01 (60 ℃) in 60 ℃ of density that are evaporated to respect to water, use macroporous resin adsorption.Carry out eluting with 30% ethanol at last, reclaim ethanol, spray drying promptly.
Above-described only is preferred implementation of the present invention, the invention is not restricted to above embodiment.Be appreciated that other improvement and variation that those skilled in the art directly derive or associate under the prerequisite that does not break away from spirit of the present invention and design, all should think to be included within protection scope of the present invention.
Claims (5)
1. the production method of the Radix Astragali, Radix Codonopsis compound extract is characterized in that processing step is as follows:
(1) Radix Astragali, Radix Codonopsis decoction pieces are soaked, used cellulase degradation 30 minutes, decoct the 30 minutes after-filtration that reflux, obtain filtrate and filtering residue;
(2) will be added the CaO alkalescent water of 4～12 times of weight by the filtering residue that step (1) obtains, the 30 minutes after-filtration that reflux obtain filtrate and filtering residue;
(3) will be added the sodium acetate aqueous solution of 4～12 times of weight by the filtering residue that step (2) obtain, the 30 minutes after-filtration that reflux obtain filtrate and filtering residue;
(4) filtrate in combining step (1), step (2) and the step (3) is 1.01 with the filtrate after merging in 60 ℃ of relative densities that are evaporated to for water, uses macroporous resin adsorption impurity;
(5) carry out eluting with 30% ethanol, reclaim ethanol, spray drying obtains finished product.
2. according to the production method of the described Radix Astragali of claim 1, Radix Codonopsis compound extract, the addition that it is characterized in that described cellulase is a 0.5kg/ ton water.
3. according to the production method of the described Radix Astragali of claim 1, Radix Codonopsis compound extract, the mass concentration that it is characterized in that described CaO alkalescent water is 1%.
4. according to the production method of the described Radix Astragali of claim 1, Radix Codonopsis compound extract, the mass concentration that it is characterized in that described sodium acetate aqueous solution is 0.5%.
5. according to the production method of the described Radix Astragali of claim 1, Radix Codonopsis compound extract, the weight proportion that it is characterized in that the Radix Astragali, Radix Codonopsis in the described Radix Astragali, the Radix Codonopsis compound extract is 4: 1.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102659656A (en) * | 2012-05-22 | 2012-09-12 | 广州中医药大学 | Method for separating dangshen alkaloid monomers from dangshen through high-speed counter-current chromatography |
| CN103053800A (en) * | 2013-01-22 | 2013-04-24 | 无锡正大畜禽有限公司 | Meat flavoring agent for livestock and poultry |
| CN103609858A (en) * | 2013-11-20 | 2014-03-05 | 宁夏天地经纬电力设备工程有限公司 | Feed additive and preparation method thereof |
| CN103734477A (en) * | 2013-12-28 | 2014-04-23 | 邵素英 | Preparation method of efficient feed additive |
| CN103749977A (en) * | 2013-12-28 | 2014-04-30 | 邵素英 | Efficient feed additive |
| CN105661069A (en) * | 2016-01-22 | 2016-06-15 | 广州暨南生物医药研究开发基地有限公司 | Feed additive for improving swine reproductive performance and preparation method thereof |
| CN110151813A (en) * | 2019-05-24 | 2019-08-23 | 重庆市科学技术研究院 | A kind of Radix Codonopsis extract and its preparation method and application |
| CN110227047A (en) * | 2019-06-26 | 2019-09-13 | 广州三广生物科技有限公司 | Skin care composition with effect of removing red blood streak and preparation method thereof |
| CN112384233A (en) * | 2019-01-31 | 2021-02-19 | 邦泰生物工程(深圳)有限公司 | A method for preparing radix Codonopsis extract from radix Codonopsis leftover |
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| CN106858598A (en) * | 2017-04-01 | 2017-06-20 | 吉林大学 | It is a kind of to specialize in micro-capsulized instant health food that puerpera eats and preparation method thereof |
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| CN100398564C (en) * | 2005-05-10 | 2008-07-02 | 上海市农业科学院 | Process for half bionic extracting preparing hedgehogt fungus crude polysaccharose |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102659656A (en) * | 2012-05-22 | 2012-09-12 | 广州中医药大学 | Method for separating dangshen alkaloid monomers from dangshen through high-speed counter-current chromatography |
| CN103053800A (en) * | 2013-01-22 | 2013-04-24 | 无锡正大畜禽有限公司 | Meat flavoring agent for livestock and poultry |
| CN103053800B (en) * | 2013-01-22 | 2014-06-18 | 无锡正大畜禽有限公司 | Meat flavoring agent for livestock and poultry |
| CN103609858A (en) * | 2013-11-20 | 2014-03-05 | 宁夏天地经纬电力设备工程有限公司 | Feed additive and preparation method thereof |
| CN103734477A (en) * | 2013-12-28 | 2014-04-23 | 邵素英 | Preparation method of efficient feed additive |
| CN103749977A (en) * | 2013-12-28 | 2014-04-30 | 邵素英 | Efficient feed additive |
| CN105661069A (en) * | 2016-01-22 | 2016-06-15 | 广州暨南生物医药研究开发基地有限公司 | Feed additive for improving swine reproductive performance and preparation method thereof |
| CN112384233A (en) * | 2019-01-31 | 2021-02-19 | 邦泰生物工程(深圳)有限公司 | A method for preparing radix Codonopsis extract from radix Codonopsis leftover |
| CN112384233B (en) * | 2019-01-31 | 2022-07-26 | 邦泰生物工程(深圳)有限公司 | A method for preparing radix Codonopsis extract from radix Codonopsis leftover |
| CN110151813A (en) * | 2019-05-24 | 2019-08-23 | 重庆市科学技术研究院 | A kind of Radix Codonopsis extract and its preparation method and application |
| CN110151813B (en) * | 2019-05-24 | 2021-06-18 | 重庆市科学技术研究院 | Codonopsis pilosula extract and preparation method and application thereof |
| CN110227047A (en) * | 2019-06-26 | 2019-09-13 | 广州三广生物科技有限公司 | Skin care composition with effect of removing red blood streak and preparation method thereof |
| CN110227047B (en) * | 2019-06-26 | 2022-05-13 | 广州三广生物科技有限公司 | Skin care composition with effect of removing red blood streak and preparation method thereof |
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Address after: The new plum village in Jiangsu province 214112 city Wuxi Qunxing Road No. 51 Patentee after: WUXI ZHENGDA BIOLOGY CO., LTD. Address before: The new plum village in Jiangsu province 214112 city Wuxi Qunxing Road No. 51 Patentee before: Wuxi Zhengda Poultry Co., Ltd. |