CN101915741B - Portable hemoglobin solution measurement system and measurement method thereof - Google Patents

Portable hemoglobin solution measurement system and measurement method thereof Download PDF

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CN101915741B
CN101915741B CN2010102462736A CN201010246273A CN101915741B CN 101915741 B CN101915741 B CN 101915741B CN 2010102462736 A CN2010102462736 A CN 2010102462736A CN 201010246273 A CN201010246273 A CN 201010246273A CN 101915741 B CN101915741 B CN 101915741B
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light
reaction solution
terminal
cuvette
frequency signal
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CN101915741A (en
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高超
李宏
王彬之
冯涛涛
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Ningbo University
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Abstract

本发明公开了一种便携式血红蛋白溶液测量系统及相应的测量方法，该测量系统包括ARM控制单元、稳定光源产生电路、比色皿、光电转换电路和A/D转换器，优点在于本测量系统根据氰化高铁血红蛋白反应溶液对不同波长光的吸收特性，由ARM控制器控制稳定光源产生电路自动分时切换产生红光和绿光，对比处理透射溶液后的红光和绿光的光强来实现氰化高铁血红蛋白反应溶液的浓度的测量，解决了现有的采用单波长法进行测量的仪器需要测量空白溶液进行对比校正的缺陷，从而大大提高了测量效率，也使得操作更为简单，同时也避免了两次测量由于外部环境及人为操作等问题造成的误差，从而有效提高了测量精度。

The invention discloses a portable hemoglobin solution measurement system and a corresponding measurement method. The measurement system includes an ARM control unit, a stable light source generation circuit, a cuvette, a photoelectric conversion circuit and an A/D converter. The advantage lies in that the measurement system is based on The absorption characteristics of the cyanide methemoglobin reaction solution to different wavelengths of light, the ARM controller controls the stable light source generation circuit to automatically switch between red and green light in time, and compares the light intensity of the red light and green light after the transmission solution is processed. The measurement of the concentration of the cyanide methemoglobin reaction solution solves the defect that the existing single-wavelength method needs to measure the blank solution for comparison and correction, thereby greatly improving the measurement efficiency and making the operation easier. The error caused by the external environment and human operation in the two measurements is avoided, thereby effectively improving the measurement accuracy.

Description

A kind of portable hemoglobin solutions measuring system and corresponding measuring method
Technical field
The present invention relates to a kind of measurement of concetration technology of hemoglobin solutions, especially relate to a kind of portable hemoglobin solutions measuring system and corresponding measuring method.
Background technology
Haemoglobin is an endoerythrocytic principal ingredient in the blood of human body; It has the easy characteristic that combines with oxygen and carbon dioxide; Be the carrier of respiratory system, can the oxygen that human body sucks be respectively organized that exhalation was external after the carbon dioxide in will organizing again was transported to lung from the pulmonary delivery to the whole body.Yet; (Hemoglobin will produce the anaemia phenomenon when HGB) being lower than normal value, and anaemia may cause human body skin, mucosal pallor, palpitating speed, sensation of oppression over the chest with shortness of breath when HC; Therefore; There is very big relation in haemoglobin with the health of human body, and people should understand the HC of oneself timely and accurately, keep the health of oneself.
The hemoglobin solutions measuring instrument is mainly used in the HC of measuring in the blood; It is widely used on clinical medicine; The main application has blood station, ICU, operating room, gynemetrics (delivery room), surgical clinical, kidney to pass through many aspects such as section, hematology, emergency ward, ambulance, neonate's monitoring and sportsman's health check-up; Become the most important diagnostic means of a lot of diseases, be the clinical accurately and timely firsthand information that provides, Medical Instruments is absolutely necessary.
At present, existing both at home and abroad multiple hemoglobin solutions measuring instrument, external Hemoglobin hemoglobinometry appearance like Sweden; It mainly adopts the reagent strip method to come hemoglobin testing concentration, and the blood sampling of this instrument is convenient, need not dilution, test is succinct, very be suitable for the training athlete scene tests, but it costs an arm and a leg; Cause middle and small hospital to purchase in batches; Agents useful for same bar price is also very high in addition, and is consumables, and this has increased patient's burden undoubtedly; The 5020 type haemoglobin appearance that the domestic close scientific instrument of as above Nereid company limited analytical instrument head factory is produced; It has adopted single wavelength method; Its light that utilizes certain wavelength is through being equipped with the cuvette of blank solution (cyaniding high ferro standard dilution); The light that utilizes identical wavelength again through be equipped with the haemoglobin reaction solution (this haemoglobin reaction solution be the cyaniding high ferro with dilution after the solution that generates of hemoglobin solutions reaction) cuvette after, to analyzing through two kinds of light intensity behind cuvette that blank solution is housed and the cuvette that the haemoglobin reaction solution is housed respectively, thereby draw the concentration of the solution of surveying; This apparatus measures time is shorter; But it needs to join diluter 250 times of hemodilutions outward, and then the blood after will dilute and cyaniding high ferro carry out hemolytic reaction as detected solution, causes the volume ten minutes of this instrument huge; Dilution and haemolysis need the long period simultaneously, make that the time of the single blood sample of actual measurement increases greatly (approximately needing 45 seconds/sample); On the other hand, compare correction owing to adopt single wavelength method need measure through the light intensity behind the cuvette that blank solution is housed, cause complicated operation, efficiency of measurement is low; In addition, twice measurement causes The ultimate results to be subject to the influence of extraneous factor (like level of skill of operator etc., extraneous dust etc.), directly influenced measuring accuracy.
Summary of the invention
Technical matters to be solved by this invention provide a kind of simple to operate, efficiency of measurement is high, measuring accuracy is high; And portable hemoglobin solutions measuring system provides a kind of measuring method that can obtain than the hemoglobin solutions concentration of high measurement accuracy simultaneously.
The present invention solves the problems of the technologies described above the technical scheme that is adopted: a kind of portable hemoglobin solutions measuring system; Comprise ARM control module, light stable source generating circuit, cuvette, photoelectric switching circuit and A/D converter; Cyanmethemoglobin reaction solution to be measured is housed in the described cuvette; The timesharing of described ARM control module is controlled described light stable source generating circuit and is produced ruddiness and the green glow with stable light intensity respectively; Ruddiness incided on the cyanmethemoglobin reaction solution to be measured in the described cuvette when described light stable source generating circuit produced ruddiness; Cyanmethemoglobin reaction solution absorption portion ruddiness to be measured in the described cuvette; Described photoelectric switching circuit receives through the red emergent light behind the cyanmethemoglobin reaction solution to be measured in the described cuvette; The light intensity of the red emergent light that described photoelectric switching circuit conversion receives is first analog voltage signal; Described photoelectric switching circuit transmits first analog voltage signal gives described A/D converter; Described A/D converter is changed the first frequency signal that first analog voltage signal is the digit pulse formula; The first frequency signal of described A/D converter transmission of digital pulsed is given described ARM control module; Green glow incided on the cyanmethemoglobin reaction solution to be measured in the described cuvette when described light stable source generating circuit produced green glow; Cyanmethemoglobin reaction solution absorption portion green glow to be measured in the described cuvette, described photoelectric switching circuit receive through the green emergent light behind the cyanmethemoglobin reaction solution to be measured in the described cuvette, and the light intensity of the green emergent light that described photoelectric switching circuit conversion receives is second analog voltage signal; Described photoelectric switching circuit transmits second analog voltage signal gives described A/D converter; Described A/D converter is changed the second frequency signal that second analog voltage signal is the digit pulse formula, and the second frequency signal of described A/D converter transmission of digital pulsed is given described ARM control module, and the first frequency signal of the digit pulse formula that described ARM control module basis receives and the second frequency signal of digit pulse formula obtain the concentration of cyanmethemoglobin reaction solution to be measured.
Described ARM control module is the microprocessor of S3C44B0X for the model based on the ARM7 kernel; The built-in timer/counter of described microprocessor is configured to the timer sum counter; Described timer intercoms with described ARM7 kernel respectively with described counter mutually; Described ARM control module is connected with keyboard, storer and display screen; Open described timer after the initialization of described ARM control module; Described timer produces first when regularly interrupting; The described light stable source generating circuit of nuclear control produces the ruddiness with stable light intensity in the described ARM7, and when second timing of described timer generation interrupted, described counter was counted the pulse number of the first frequency signal of digit pulse formula; Described timer produces the 3rd timing when interrupting; Described counter is transferred to described ARM7 kernel with its count value that pulse number of the first frequency signal of digit pulse formula is counted and handles, and simultaneously the described light stable source generating circuit of nuclear control produces the green glow with stable light intensity in the described ARM7, and described timer produces the 4th timing when interrupting; Described counter is counted the pulse number of the second frequency signal of digit pulse formula; Described timer produces the 5th timing when interrupting, and described counter is transferred to described ARM7 kernel with its count value that pulse number of the second frequency signal of digit pulse formula is counted and handles, and described ARM7 kernel calculates the concentration of cyanmethemoglobin reaction solution to be measured according to two count values; Described ARM7 kernel is stored the concentration of cyanmethemoglobin reaction solution to be measured to described storer, and the concentration of transmitting cyanmethemoglobin reaction solution to be measured simultaneously shows to described display screen.
Described light stable source generating circuit mainly is made up of voltage regulation unit, control module and luminescence unit; Described voltage regulation unit comprises source of stable pressure, first electrochemical capacitor, second electric capacity, the 3rd electrochemical capacitor, the 4th electric capacity and first resistance; Described control module comprises first triode, second triode, second resistance and the 3rd resistance; Described luminescence unit comprises red light emitting diodes and green LED; Described source of stable pressure has voltage input end, voltage output end and earth terminal; The voltage input end cut-in operation voltage of described source of stable pressure; The voltage input end of described source of stable pressure is connected with the positive terminal of described first electrochemical capacitor and first end of described second electric capacity respectively; The equal ground connection of second end of the negative pole end of described first electrochemical capacitor and described second electric capacity; The voltage output end of described source of stable pressure is connected with the positive terminal of first end of described first resistance, described the 3rd electrochemical capacitor and first end of described the 4th electric capacity respectively; The earth terminal of described source of stable pressure is connected with the negative pole end of second end of described first resistance, described the 3rd electrochemical capacitor and second end of described the 4th electric capacity respectively; Described ARM control module is connected with first end of described second resistance through first connection terminal; Second end of described second resistance is connected with the base stage of described first triode; The collector of described first triode is connected with the negative pole end of described red light emitting diodes; Described ARM control module is connected with first end of described the 3rd resistance through second connection terminal, and second end of described the 3rd resistance is connected with the base stage of described second triode, and the collector of described second triode is connected with the negative pole end of described green LED; The equal ground connection of the emitter of the emitter of described first triode and described second triode; The positive terminal of described red light emitting diodes all is connected with the earth terminal of described source of stable pressure with the positive terminal of described green LED, and the ruddiness that described red light emitting diodes sends incides on the cyanmethemoglobin reaction solution to be measured in the described cuvette through a sidewall of described cuvette, and the green glow that described green LED sends incides on the cyanmethemoglobin reaction solution to be measured in the described cuvette through a sidewall of described cuvette.
It is the low-power consumption forward voltage regulator of SPX1117 that described source of stable pressure adopts model, and the voltage of the voltage output end output of described low-power consumption forward voltage regulator is 5V; Described first triode and described second triode all adopt NPN type triode.
When described first connection terminal inserts high level and described second connection terminal access low level; Described first triode is in state of saturation; Described second triode is in cut-off state; Described red light emitting diodes sends ruddiness, and the ruddiness that described red light emitting diodes sends incides on the cyanmethemoglobin reaction solution to be measured in the described cuvette through a sidewall of described cuvette; When described first connection terminal inserts low level and described second connection terminal access high level; Described first triode is in cut-off state; Described second triode is in state of saturation; Described green LED sends green glow, and the green glow that described green LED sends incides on the cyanmethemoglobin reaction solution to be measured in the described cuvette through a sidewall of described cuvette.
Described photoelectric switching circuit mainly is made up of photodiode, the 4th resistance, the 5th electric capacity, first sliding device, the second sliding change device and the operational amplifier of becoming; Described photodiode receives through red emergent light and green emergent light behind the cyanmethemoglobin solution to be measured in the described cuvette; The positive terminal ground connection of described photodiode; The positive terminal of described photodiode is connected with the normal phase input end of described operational amplifier; The negative pole end of described photodiode is connected with first end of first end of described the 4th resistance, described the 5th electric capacity and the inverting input of described operational amplifier respectively; Second end of described the 4th resistance is connected with described first sliding first end that becomes device; Described first sliding second end that becomes device is connected with the described first sliding slide plate that becomes device; Its public connecting end is connected with second end of described the 5th electric capacity and the output terminal of described operational amplifier respectively; The output terminal of described operational amplifier is connected with the input end of described A/D converter; The first zeroing end of described operational amplifier is connected with described second sliding first end that becomes device, and the second zeroing end of described operational amplifier is connected with described second sliding second end that becomes device, and the positive power source terminal that connects of described operational amplifier is connected with the described second sliding slide plate that becomes device.
The model of described operational amplifier is OP07/AD705.
Described A/D converter adopts voltage-frequency changing type A/D converter.
A kind of above-mentioned corresponding measuring method of portable hemoglobin solutions measuring system may further comprise the steps:
1. initialization ARM control module; The built-in timer of ARM control module is unlocked; It produces first and regularly interrupts; First triode that the ARM7 kernel control stabilization light source of ARM control module produces circuit makes it be in state of saturation, and the red light emitting diodes that the first triode work control stabilization light source produces circuit sends the ruddiness with stable light intensity;
2. the ruddiness that sends of red light emitting diodes incides on the cyanmethemoglobin reaction solution to be measured in the cuvette; Cyanmethemoglobin reaction solution absorption portion ruddiness to be measured; And shine on the photodiode of photoelectric switching circuit through the red emergent light behind the cyanmethemoglobin reaction solution to be measured; Photodiode converts the light intensity of red emergent light into first analog voltage signal; The operational amplifier of photoelectric switching circuit carries out processing and amplifying to first analog voltage signal, and first analog voltage signal after will amplifying is transferred to A/D converter;
3. A/D converter converts first analog voltage signal into the first frequency signal of digit pulse formula; And the first frequency signal of digit pulse formula is transferred to the counter of ARM control module; When second timing of timer generation simultaneously interrupted, counter was counted the pulse number of the first frequency signal of digit pulse formula;
4. timer produces the 3rd timing when interrupting; Counter is transferred to the ARM7 kernel with its count value that pulse number of the first frequency signal of digit pulse formula is counted and handles; Second triode of the circuit of ARM7 kernel control stabilization light source generation simultaneously makes it be in state of saturation, and the green LED that the second triode work control stabilization light source produces circuit sends the green glow with stable light intensity;
5. the green glow that sends of green LED incides on the cyanmethemoglobin reaction solution to be measured in the cuvette; Cyanmethemoglobin reaction solution absorption portion green glow to be measured; And shine on the photodiode of photoelectric switching circuit through the green emergent light behind the cyanmethemoglobin reaction solution to be measured; Photodiode converts the light intensity of green emergent light into second analog voltage signal; The operational amplifier of photoelectric switching circuit carries out processing and amplifying to second analog voltage signal, and second analog voltage signal after will amplifying is transferred to A/D converter;
6. A/D converter converts second analog voltage signal into the second frequency signal of digit pulse formula; And the second frequency signal of digit pulse formula is transferred to the counter of ARM control module; Simultaneously timer produces the 4th timing when interrupting, and counter is counted the pulse number of the second frequency signal of digit pulse formula;
7. timer produces the 5th timing when interrupting, and counter is transferred to the ARM7 kernel with its count value that pulse number of the second frequency signal of digit pulse formula is counted and handles;
8. the ARM7 kernel calculates the concentration of cyanmethemoglobin reaction solution to be measured according to two count values, is designated as C,
Figure BSA00000218590500051
Wherein, K 1The absorption coefficient of expression ruddiness, K 2The absorption coefficient of expression green glow, L representes the thickness of a sidewall of cuvette, f 1The count value that the expression counter counts to get the pulse number of the first frequency signal of digit pulse formula, f 2The count value that the expression counter counts to get the pulse number of the second frequency signal of digit pulse formula, L In1The light intensity of the ruddiness that the red light emitting diodes of expression light stable source generating circuit sends, L In2The light intensity of the green glow that the green LED of expression light stable source generating circuit sends.
Make in the above-mentioned measuring method and be a; Make
Figure BSA00000218590500053
and be b; Make
Figure BSA00000218590500054
and be x; 1. organize cyanmethemoglobin reaction solution to be measured corresponding x and C to 8. measuring according to step more; Utilize approximating method to simulate the occurrence of a and b then, finally obtain the concentration
Figure BSA00000218590500055
of cyanmethemoglobin reaction solution to be measured
Compared with prior art; The invention has the advantages that this measuring system is according to the absorption characteristic of cyanmethemoglobin reaction solution to different wavelengths of light; Produce the automatic timesharing of circuit by ARM controller control stabilization light source and switch generation ruddiness and green glow; The ruddiness behind the contrast processing transmission solution and the light intensity of green glow realize the measurement of the concentration of cyanmethemoglobin reaction solution; Solve the appliance requires that the single wavelength method of existing employing measures and measured the defective that blank solution compares correction; Thereby improved efficiency of measurement greatly; Make that also operation is more simple, also avoided twice measurement (promptly to the light intensity of the light that sees through the cuvette that blank solution is housed and see through the light intensity of the light of the cuvette that reaction solution is housed) simultaneously because the error that problems such as external environment condition and manual operation cause, thereby effectively improved measuring accuracy; This measuring system is compared to the high hemoglobinometry appearance of external price; It need not the reagent strip of disposable consumption, and its ARM control module with Highgrade integration is that core adds each element circuit, and is not only cheap; And good reproducibility, be suitable for hospital and purchase in batches; And compared to domestic part main equipment, this measuring system is owing to adopt Embedded ARM control module to handle, and do not need to join diluter and carry out hemodilution outward, makes the miniaturization of this measuring system, is convenient to pay a home visit and carries.This measuring system cooperates display screen to show synchronously through keyboard operation, makes this measuring system humanized, operates simpler.
This measuring method adopts the double wave regular way promptly to utilize two ruddiness with different wave length and green glow respectively through the cyanmethemoglobin reaction solution is housed; The measurement of realization response solution concentration; Solve the single wavelength method of existing employing and need measure the defective that blank solution compares correction; Thereby improved efficiency of measurement greatly, made that also operation is more simple, also avoided twice measurement simultaneously because the error that problems such as external environment condition and manual operation cause; Thereby effectively improved measuring accuracy, can satisfy clinical medical needs preferably.This measurement method selection ruddiness and green glow come through reaction solution; This is because ruddiness and green glow have close absorbance at two wavelength interfering components; And the absorbance at two wavelength reaction solutions to be measured has enough big difference; Photoelectric switching circuit has higher relative sensitivity to these two kinds of light, can effectively reduce like this and measure the error of bringing, thereby improve measuring accuracy.
Description of drawings
Fig. 1 is the fundamental block diagram of portable hemoglobin solutions measuring system of the present invention;
Fig. 2 is the circuit diagram of light stable source generating circuit;
Fig. 3 is the circuit diagram of photoelectric switching circuit;
Fig. 4 is the absorbance log characteristic synoptic diagram of light in the cyanmethemoglobin reaction solution;
Fig. 5 is the synoptic diagram that concerns of wavelength and relative sensitivity.
Embodiment
Embodiment describes in further detail the present invention below in conjunction with accompanying drawing.
Embodiment one:
A kind of portable hemoglobin solutions measuring system that the present invention proposes; As shown in Figure 1; It comprises ARM control module 1, light stable source generating circuit 2, cuvette 3, photoelectric switching circuit 4 and A/D converter 5; Cyanmethemoglobin reaction solution (not shown) to be measured is housed in the cuvette 3; ARM control module 1 timesharing control stabilization light source produces circuit 2 and produces ruddiness and the green glow with stable light intensity respectively; Ruddiness incided on the cyanmethemoglobin reaction solution to be measured in the cuvette 3 when light stable source generating circuit 2 produced ruddiness; Cyanmethemoglobin reaction solution absorption portion ruddiness to be measured in the cuvette 3; It is the remaining transmit red light that is not absorbed by cyanmethemoglobin reaction solution to be measured that photoelectric switching circuit 4 receives through the red emergent light behind the cyanmethemoglobin reaction solution to be measured in the cuvette 3; The light intensity of the red emergent light that photoelectric switching circuit 4 conversions receive is first analog voltage signal; Photoelectric switching circuit 4 transmission first analog voltage signal is given A/D converter 5; A/D converter 5 conversions first analog voltage signal is the first frequency signal of digit pulse formula; The first frequency signal of A/D converter 5 transmission of digital pulseds is given ARM control module 1, and green glow incided on the cyanmethemoglobin reaction solution to be measured in the cuvette 3 when light stable source generating circuit 2 produced green glow, the cyanmethemoglobin reaction solution absorption portion green glow to be measured in the cuvette 3; It is the remaining transmit green that is not absorbed by cyanmethemoglobin reaction solution to be measured that photoelectric switching circuit 4 receives through the green emergent light behind the cyanmethemoglobin reaction solution to be measured in the cuvette 3; The light intensity of the green emergent light that photoelectric switching circuit 4 conversions receive is second analog voltage signal, and photoelectric switching circuit 4 transmission second analog voltage signal is given A/D converter 5, and A/D converter is changed the second frequency signal that 5 second analog voltage signals are the digit pulse formula; The second frequency signal of A/D converter 5 transmission of digital pulseds is given ARM control module 1, and the first frequency signal of the digit pulse formula that ARM control module 1 basis receives and the second frequency signal of digit pulse formula obtain the concentration of cyanmethemoglobin reaction solution to be measured.
In this specific embodiment; Cyanmethemoglobin reaction solution to be measured is generated by cyaniding high iron solution and haemoglobin reaction; It need not, and the outer diluter of joining because single wavelength method is the contrast correcting fluid with the blank solution, is difficult to guarantee measuring accuracy to 250 times of hemodilutions without dilution as 5020 type haemoglobin appearance; And this measuring system is with the same solution of the transmittance of two kinds of wavelength; With the cyanmethemoglobin reaction solution to the different absorbance of two kinds of wavelength as beasurement base, had higher measuring accuracy, if dilution also can improve measuring accuracy certainly.
In this specific embodiment; ARM control module 1 is the microprocessor of S3C44B0X for the model based on ARM7 kernel 11; ARM control module 1 is that the built-in timer/counter of microprocessor is configurable for timer 12 sum counters 13; Timer 12 sum counters 13 intercom with ARM7 kernel 11 respectively mutually; ARM control module 1 is connected with keyboard 14, storer 15 and display screen 16; Opening timing device 12 after 1 initialization of ARM control module; Timer 12 produces first when regularly interrupting; ARM7 kernel 11 reaches the I/O mouth (not shown) output low level that is connected with the second connection terminal GreOn of light stable source generating circuit 2 through the I/O mouth (not shown) output high level that is connected with first connection terminal RedOn light stable source generating circuit 2 ARM control module 1 and comes the control stabilization light source to produce the ruddiness that circuit 2 generations have stable light intensity; When second timing of timer 12 generations interrupted; The pulse number of the first frequency signal of 13 pairs of digit pulse formulas of counter is counted; When the 3rd timing of timer 12 generations interrupted; Counter 13 is transferred to ARM7 kernel 11 with its count value that pulse number of the first frequency signal of digit pulse formula is counted and handles, and while ARM7 kernel 11 reaches the I/O mouth (not shown) output high level that is connected with the second connection terminal GreOn of light stable source generating circuit 2 through the I/O mouth (not shown) output low level that is connected with first connection terminal RedOn light stable source generating circuit 2 ARM control module 1 and comes the control stabilization light source to produce the green glow that circuit 2 generations have stable light intensity, when the 4th timing of timer 12 generations interrupted; The pulse number of the second frequency signal of 13 pairs of digit pulse formulas of counter is counted; When the 5th timing of timer 12 generations interrupted, counter 13 was transferred to ARM7 kernel 11 with its count value that pulse number of the second frequency signal of digit pulse formula is counted and handles, and ARM7 kernel 11 calculates the concentration of cyanmethemoglobin reaction solution to be measured according to two count values; The concentration of ARM7 kernel 11 storages cyanmethemoglobin reaction solution to be measured is to storer 15, and the concentration of transmitting cyanmethemoglobin reaction solution to be measured simultaneously shows to display screen 16.Display screen 16 shows master menu after 1 initialization of ARM control module; Can carry out function selecting through keyboard 14; Keyboard 14 each key function corresponding can be according to the actual conditions setting; As 1 key can be set the crowd of under-18s is measured, 2 keys can be set 18～60 years old crowd is measured, 3 keys can be set the crowd more than 60 years old is measured or the like.Timer 12 sum counters 13 obtain for S3C44B0X inside integrated " timer/counter " block configuration; Control their open and close and interruption is set by ARM7 kernel 11; The foundation of timing and counting process is ARM7 kernel 11 inner crystal oscillators or external pulse (like the frequency signal after the conversion); Up to interruption generating, how the value that perhaps count down to some settings operates if being decided by ARM7 kernel 11 again.
In this specific embodiment; Light stable source generating circuit 2 is as shown in Figure 2; It mainly is made up of voltage regulation unit, control module and luminescence unit; Voltage regulation unit comprises source of stable pressure U1, the first electrochemical capacitor C1, second capacitor C 2, the 3rd electrochemical capacitor C3, the 4th capacitor C 4 and first resistance R 1; Control module comprises the first triode Q1, the second triode Q2, second resistance R 2 and the 3rd resistance R 3; Luminescence unit comprises red light emitting diodes D1 and green LED D2; Source of stable pressure U1 has voltage input end VIN, voltage output end VOUT and earth terminal GND; The voltage input end VIN cut-in operation voltage 15V of source of stable pressure U1; The voltage input end VIN of source of stable pressure U1 is connected with the positive terminal of the first electrochemical capacitor C1 and first end of second capacitor C 2 respectively; The equal ground connection of second end of the negative pole end of the first electrochemical capacitor C1 and second capacitor C 2, the voltage output end VOUT of source of stable pressure U1 is connected with first end of first resistance R 1, the positive terminal of the 3rd electrochemical capacitor C3 and first end of the 4th capacitor C 4 respectively, and the earth terminal GND of source of stable pressure U1 is connected with second end of first resistance R 1, the negative pole end of the 3rd electrochemical capacitor C3 and second end of the 4th capacitor C 4 respectively; ARM control module 1 is connected with first end of second resistance R 2 through the first connection terminal RedOn; Second end of second resistance R 2 is connected with the base stage of the first triode Q1, and the collector of the first triode Q1 is connected with the negative pole end of red light emitting diodes D1, and ARM control module 1 is connected with first end of the 3rd resistance R 3 through the second connection terminal GreOn; Second end of the 3rd resistance R 3 is connected with the base stage of the second triode Q2; The collector of the second triode Q2 is connected with the negative pole end of green LED D2, the equal ground connection of emitter of the emitter of the first triode Q1 and the second triode Q2, and the positive terminal of the positive terminal of red light emitting diodes D1 and green LED D2 all is connected with the earth terminal GND of source of stable pressure U1; The ruddiness that red light emitting diodes D1 sends incides on the cyanmethemoglobin reaction solution to be measured in the cuvette 3 through a sidewall of cuvette 3, and the green glow that green LED D2 sends incides on the cyanmethemoglobin reaction solution to be measured in the cuvette 3 through a sidewall of cuvette 3.At this; It is the low-power consumption forward voltage regulator of SPX1117 that source of stable pressure U1 adopts model; The voltage of the voltage output end of low-power consumption forward voltage regulator output is 5V, and also can adopting other, ripe arbitrarily to have with model be the pressure stabilizing device of the low-power consumption forward voltage regulator identical function of SPX1117; The first triode Q1 and the second triode Q2 all adopt NPN type triode; The first connection terminal RedOn inserts high level and the second connection terminal GreOn inserts low level; The first triode Q1 is in state of saturation; The second triode Q2 is in cut-off state, and red light emitting diodes D1 sends ruddiness, and the ruddiness that red light emitting diodes D1 sends incides on the cyanmethemoglobin reaction solution to be measured in the cuvette 3 through a sidewall of cuvette 3; When the first connection terminal RedOn inserts low level and second connection terminal GreOn access high level; The first triode Q1 is in cut-off state; The second triode Q2 is in state of saturation; Green LED D2 sends green glow, and the green glow that green LED D2 sends incides on the cyanmethemoglobin reaction solution to be measured in the cuvette 3 through a sidewall of cuvette 3.
In fact; Voltage regulation unit also can directly adopt voltage stabilizing diode, utilize the burning voltage of voltage stabilizing diode to produce steady current, yet the voltage regulation performance of voltage stabilizing diode can change along with the influence of factors such as temperature; Thereby the electric current that flows through red light emitting diodes or green LED also can change along with variation of temperature; Influence the accuracy of measuring the most at last, therefore in the present embodiment, it is the low-power consumption forward voltage regulator of SPX1117 that source of stable pressure adopts model; This source of stable pressure can be according to the automatic regulation voltage of the variation of environment; Make voltage stable, thereby make the current stabilization that flows through red light emitting diodes or green LED, reach the purpose that the light that red light emitting diodes and green LED are sent has stable light intensity.
In this specific embodiment; Photoelectric switching circuit mainly is made up of photodiode D3, the 4th resistance R 4, the 5th capacitor C 5, first sliding device VR1, the second sliding device VR2 of change and the operational amplifier U4 of becoming; Photodiode D3 receives through red emergent light and green emergent light behind the cyanmethemoglobin solution to be measured in the cuvette 3; The positive terminal ground connection of photodiode D3; The positive terminal of photodiode D3 is connected with the normal phase input end of operational amplifier U4; The negative pole end of photodiode D3 is connected with first end of the 4th resistance R 4, first end of the 5th capacitor C 5 and the inverting input of operational amplifier U4 respectively; Second end of the 4th resistance R 4 is connected with first sliding first end that becomes device VR1, and first sliding second end that becomes device VR1 is connected with the first sliding slide plate that becomes device VR1, and its public connecting end is connected with second end of the 5th capacitor C 5 and the output terminal of operational amplifier U4 respectively; The output terminal of operational amplifier U4 is connected with the input end of A/D converter 5; The first zeroing end of operational amplifier U4 is connected with second sliding first end that becomes device VR2, and the second zeroing end of operational amplifier U4 is connected with second sliding second end that becomes device VR2, and the positive power source terminal that connects of operational amplifier U4 is connected with the second sliding slide plate that becomes device VR2.As can be seen from Figure 3, when illumination was mapped to photodiode D3, photodiode D3 produced electric current I, and electric current I flows through the 4th resistance R 4 and the first sliding device VR1 that becomes, at the output terminal generation negative voltage U of operational amplifier U4 0, the light wavelength that order shines photodiode D3 is that λ and light intensity are I (λ), the short circuit current that makes photodiode D3 is i Sc, then photodiode D3 is the susceptibility of the light of λ to this wavelength
Figure BSA00000218590500091
I is arranged then Sc=S (λ) * I (λ), the short-circuit current i of visible light electric diode D3 ScBe directly proportional with the light intensity I (λ) of the light that shines photodiode D3, again because the negative voltage U of the output terminal of operational amplifier U4 output 0=i Sc* (R 4+ VR 1), wherein, R 4The resistance value of representing the 4th resistance R 4, VR 1The expression first sliding resistance value that becomes device VR1 is so the output terminal of light intensity I (λ) and operational amplifier U4 produces negative voltage U 0Be directly proportional, thus the negative voltage U of output terminal output that can be through measuring operational amplifier U4 0Measure the light intensity I (λ) of the light that photodiode D3 received.
At this, it is the operational amplifier of OP07/AD705 that operational amplifier U4 adopts model.
In this specific embodiment; A/D converter 5 can adopt direct A/D converter; Also can adopt voltage-time conversion hysteria (V-T) A/D converter or voltage-frequency changing type (V-F) A/D converter in the indirect A/D converter; But owing to advantages such as voltage-frequency changing type (V-F) A/D converter have working stability, good linearity, precision is high, circuit is simple, antijamming capability is strong; Therefore adopt voltage-frequency changing type (V-F) A/D converter at this; Selecting model for use is the chip of AD654, and its first analog voltage signal of mainly photoelectric switching circuit being exported converts the first frequency signal of digit pulse formula linearly to, second analog voltage signal of photoelectric switching circuit output is converted linearly to the second frequency signal of digit pulse formula.
Embodiment two:
A kind of above-mentioned corresponding measuring method of portable hemoglobin solutions measuring system, it may further comprise the steps:
1. initialization ARM control module 1; ARM control module 1 is that the built-in timer of microprocessor 12 is unlocked; It produces first and regularly interrupts; ARM7 kernel 11 makes it be in state of saturation through the first triode Q1 that the I/O mouth output high level that is connected with first connection terminal RedOn light stable source generating circuit 2 ARM control module 1 comes the control stabilization light source to produce circuit 2, and the red light emitting diodes that first triode Q1 work control stabilization light source produces circuit 2 sends the ruddiness with stable light intensity.
2. the ruddiness that sends of red light emitting diodes D1 incides on the cyanmethemoglobin reaction solution to be measured in the cuvette 3; Cyanmethemoglobin reaction solution absorption portion ruddiness to be measured; And shine on the photodiode D3 of photoelectric switching circuit 4 through the red emergent light behind the cyanmethemoglobin reaction solution to be measured; Photodiode D3 converts the light intensity of red emergent light into first analog voltage signal; The operational amplifier U4 of photoelectric switching circuit 4 carries out processing and amplifying to first analog voltage signal, and first analog voltage signal after will amplifying is transferred to A/D converter 5.
3. A/D converter 5 converts first analog voltage signal into the first frequency signal of digit pulse formula; And the first frequency signal of digit pulse formula is transferred to the counter 13 of ARM control module 1; When second timing of timer 12 generations simultaneously interrupted, the pulse number of the first frequency signal of 13 pairs of digit pulse formulas of counter was counted.
When 4. the 3rd timing of timer 12 generations interrupted; Counter 13 is transferred to ARM7 kernel 11 with its count value that pulse number of the first frequency signal of digit pulse formula is counted and handles; ARM7 kernel 11 makes it be in state of saturation through the second triode Q2 that the I/O mouth output high level that is connected with second connection terminal GreOn light stable source generating circuit 2 ARM control module 1 comes the control stabilization light source to produce circuit 2 simultaneously, and the green LED that second triode Q2 work control stabilization light source produces circuit 2 sends the green glow with stable light intensity.
5. the green glow that sends of green LED D2 incides on the cyanmethemoglobin reaction solution to be measured in the cuvette 3; Cyanmethemoglobin reaction solution absorption portion green glow to be measured; And shine on the photodiode D3 of photoelectric switching circuit 4 through the green emergent light behind the cyanmethemoglobin reaction solution to be measured; Photodiode D3 converts the light intensity of green emergent light into second analog voltage signal; The operational amplifier U4 of photoelectric switching circuit 4 carries out processing and amplifying to second analog voltage signal, and second analog voltage signal after will amplifying is transferred to A/D converter 5.
6. A/D converter 5 converts second analog voltage signal into the second frequency signal of digit pulse formula; And the second frequency signal of digit pulse formula is transferred to the counter 13 of ARM control module 1; When the 4th timing of timer 12 generations simultaneously interrupted, the pulse number of the second frequency signal of 13 pairs of digit pulse formulas of counter was counted.
When 7. the 5th timing of timer 12 generations interrupted, counter 13 was transferred to ARM kernel 11 with its count value that pulse number of the second frequency signal of digit pulse formula is counted and handles.
8. ARM7 kernel 11 calculates the concentration of cyanmethemoglobin reaction solution to be measured according to two count values, is designated as C,
Figure BSA00000218590500111
Wherein, K 1The absorption coefficient of expression ruddiness, K 2The absorption coefficient of expression green glow, L representes the thickness of a sidewall of cuvette, f 1The count value that the expression counter counts to get the pulse number of the first frequency signal of digit pulse formula, f 2The count value that the expression counter counts to get the pulse number of the second frequency signal of digit pulse formula, L In1The light intensity of the ruddiness that the red light emitting diodes of expression light stable source generating circuit sends, L In2The light intensity of the green glow that the green LED of expression light stable source generating circuit sends.
Make
Figure BSA00000218590500112
and be a; Make
Figure BSA00000218590500113
and be b; Make
Figure BSA00000218590500114
and be x; 1. organize cyanmethemoglobin reaction solution to be measured corresponding x and C to 8. measuring according to step more; Utilize existing approximating method to simulate the occurrence of a and b then, finally obtain the concentration
Figure BSA00000218590500115
of cyanmethemoglobin reaction solution to be measured
The concentration of the cyanmethemoglobin reaction solution that records with semi-automatic blood analyser F820 is below come the occurrence of match a and b as standard.At first, utilize injector for medical purpose that cyanmethemoglobin reaction solution to be measured is slowly squeezed in the cuvette, pushing syringe is six times continuously; For every pushing syringe once; Once measure accordingly, note six times x value, measurement data is as shown in table 1.
The relation table of the concentration of table 1 cyanmethemoglobin reaction solution and
Figure BSA00000218590500116
Figure BSA00000218590500121
Mean value with x is transverse axis; Corresponding C is the longitudinal axis; Set up rectangular coordinate system the draw concentration of reaction solution and the relation curve of
Figure BSA00000218590500122
; Both are linear; Occurrence with Matlab match a and b; Obtain a=-1376, b=889.8, thus the concentration that can draw the cyanmethemoglobin reaction solution is:
Figure BSA00000218590500123
Because
Figure BSA00000218590500124
and the concentration C of cyanmethemoglobin reaction solution are linear, therefore can confirm the stability of measuring system of the present invention and measuring method through the stability of measuring
Figure BSA00000218590500125
.Table 2 has provided same sample has been carried out 14 measurements, the data of of record.
The value record table of
Figure BSA00000218590500131
that table 2 is measured for 14 times
Figure BSA00000218590500132
From table 2, can draw, Mean value be 0.6380,
Figure BSA00000218590500134
Variance be 0.455 * 10 -6Because variance is the size of expression measured value deviation average; And the variance of
Figure BSA00000218590500135
is very little, and the stability of visible measuring system of the present invention and measuring method better.
This measurement method selection ruddiness and green glow are through cyanmethemoglobin reaction solution to be measured, and its reason is:
Suppose that two kinds of light wavelengths selecting are respectively λ 1And λ 2, then need guarantee wavelength X 1And λ 2Place's interfering component should have identical absorbance, could guarantee A like this S1=A S2, improve the precision of measuring; In two selected wavelength X 1And λ 2The absorbance of locating component to be measured should have enough big difference, otherwise The expression wavelength is λ 1For light through cyanmethemoglobin reaction solution to be measured after output light light intensity, I Out(λ 2) the expression wavelength is λ 2For light through cyanmethemoglobin reaction solution to be measured after output light light intensity) value very little, can bring very big error to measurement; Wavelength X 1And λ 2The photodiode of place's photoelectric switching circuit will have enough relative sensitivity, could improve the sensitivity of measuring system like this.Can find out that according to table 3 the highest for the absorption of ruddiness, blue light and green glow, the wavelength of blue light is 470-480nm, the wavelength of green glow is 530-540nm.
Table 3 material color and the complementary relationship that absorbs look
Figure BSA00000218590500141
Can know from Fig. 4; Cyanmethemoglobin reaction solution to be measured to blue light and green glow absorbance log all than higher; Can satisfy the requirement of this test macro and measuring method, but as can beappreciated from fig. 5, the relative sensitivity of blue light is very low; And the relative sensitivity of green glow is approximately 2 times of blue light, so this method of testing selects ruddiness and green glow as measurement light source.

Claims (6)

1.一种便携式血红蛋白溶液测量系统，其特征在于包括ARM控制单元、稳定光源产生电路、比色皿、光电转换电路和A/D转换器，所述的比色皿内装有待测氰化高铁血红蛋白反应溶液，所述的ARM控制单元分时控制所述的稳定光源产生电路分别产生具有稳定光强的红光和绿光，所述的稳定光源产生电路产生红光时红光入射到所述的比色皿内的待测氰化高铁血红蛋白反应溶液上，所述的比色皿内的待测氰化高铁血红蛋白反应溶液吸收部分红光，所述的光电转换电路接收通过所述的比色皿内的待测氰化高铁血红蛋白反应溶液后的红色出射光，所述的光电转换电路转换接收到的红色出射光的光强为第一模拟电压信号，所述的光电转换电路传输第一模拟电压信号给所述的A/D转换器，所述的A/D转换器转换第一模拟电压信号为数字脉冲式的第一频率信号，所述的A/D转换器传输数字脉冲式的第一频率信号给所述的ARM控制单元，所述的稳定光源产生电路产生绿光时绿光入射到所述的比色皿内的待测氰化高铁血红蛋白反应溶液上，所述的比色皿内的待测氰化高铁血红蛋白反应溶液吸收部分绿光，所述的光电转换电路接收通过所述的比色皿内的待测氰化高铁血红蛋白反应溶液后的绿色出射光，所述的光电转换电路转换接收到的绿色出射光的光强为第二模拟电压信号，所述的光电转换电路传输第二模拟电压信号给所述的A/D转换器，所述的A/D转换器转换第二模拟电压信号为数字脉冲式的第二频率信号，所述的A/D转换器传输数字脉冲式的第二频率信号给所述的ARM控制单元，所述的ARM控制单元根据接收到的数字脉冲式的第一频率信号和数字脉冲式的第二频率信号获得待测氰化高铁血红蛋白反应溶液的浓度；1. A portable hemoglobin solution measurement system, characterized in that it comprises an ARM control unit, a stable light source generating circuit, a cuvette, a photoelectric conversion circuit and an A/D converter, and the high iron cyanide to be measured is housed in the described cuvette Hemoglobin reaction solution, the ARM control unit time-sharingly controls the stable light source generating circuit to generate red light and green light with stable light intensity respectively, when the stable light source generating circuit generates red light, the red light is incident on the On the cyanomethemoglobin reaction solution to be measured in the cuvette, the cyanomethemoglobin reaction solution to be measured in the cuvette absorbs part of the red light, and the photoelectric conversion circuit receives the light through the colorimetric The red outgoing light after the cyanmethemoglobin reaction solution to be tested in the dish, the light intensity of the red outgoing light received by the photoelectric conversion circuit is converted into a first analog voltage signal, and the photoelectric conversion circuit transmits the first analog voltage signal. The voltage signal is sent to the A/D converter, and the A/D converter converts the first analog voltage signal into a digital pulse-type first frequency signal, and the A/D converter transmits the digital pulse-type first frequency signal A frequency signal is given to the ARM control unit, and when the stable light source generating circuit generates green light, the green light is incident on the cyanmethemoglobin reaction solution to be measured in the cuvette, and the cuvette The cyanomethemoglobin reaction solution to be measured in the cuvette absorbs part of the green light, and the photoelectric conversion circuit receives the green outgoing light passing through the cyanomethemoglobin reaction solution to be measured in the cuvette, and the photoelectric conversion The circuit converts the received light intensity of the green outgoing light into a second analog voltage signal, and the photoelectric conversion circuit transmits the second analog voltage signal to the A/D converter, and the A/D converter converts the first The second analog voltage signal is a digital pulse-type second frequency signal, and the A/D converter transmits a digital pulse-type second frequency signal to the ARM control unit, and the ARM control unit The first frequency signal of the pulse type and the second frequency signal of the digital pulse type obtain the concentration of the cyanmethemoglobin reaction solution to be tested; 所述的ARM控制单元为基于ARM7内核的型号为S3C44B0X的微处理器，所述的微处理器内置的定时器/计数器配置为定时器和计数器，所述的定时器和所述的计数器分别与所述的ARM7内核相互通信，所述的ARM控制单元连接有键盘、存储器和显示屏，所述的ARM控制单元初始化后开启所述的定时器，所述的定时器产生第一个定时中断时，所述的ARM7内核控制所述的稳定光源产生电路产生具有稳定光强的红光，所述的定时器产生第二个定时中断时，所述的计数器对数字脉冲式的第一频率信号的脉冲个数进行计数，所述的定时器产生第三个定时中断时，所述的计数器将其对数字脉冲式的第一频率信号的脉冲个数进行计数的计数值传输给所述的ARM7内核进行处理，同时所述的ARM7内核控制所述的稳定光源产生电路产生具有稳定光强的绿光，所述的定时器产生第四个定时中断时，所述的计数器对数字脉冲式的第二频率信号的脉冲个数进行计数，所述的定时器产生第五个定时中断时，所述的计数器将其对数字脉冲式的第二频率信号的脉冲个数进行计数的计数值传输给所述的ARM7内核进行处理，所述的ARM7内核根据两个计数值计算得到待测氰化高铁血红蛋白反应溶液的浓度，所述的ARM7内核存储待测氰化高铁血红蛋白反应溶液的浓度到所述的存储器，同时传输待测氰化高铁血红蛋白反应溶液的浓度到所述的显示屏上进行显示；Described ARM control unit is the microprocessor that the model based on ARM7 kernel is S3C44B0X, and the built-in timer/counter of described microprocessor is configured as timer and counter, and described timer and described counter are respectively connected with Described ARM7 core communicates with each other, and described ARM control unit is connected with keyboard, memory and display screen, and described ARM control unit opens described timer after initialization, and when described timer produces the first timing interrupt , the ARM7 core controls the stable light source generating circuit to generate red light with stable light intensity, and when the timer generates a second timing interrupt, the counter is to the first frequency signal of the digital pulse type The number of pulses is counted, and when the timer generates the third timing interrupt, the counter transmits the count value of counting the number of pulses of the first frequency signal of the digital pulse formula to the ARM7 core process, while the ARM7 core controls the stable light source generating circuit to produce green light with a stable light intensity, and when the timer generates the fourth timing interrupt, the counter responds to the second digital pulse type The number of pulses of the frequency signal is counted, and when the timer generates a fifth timing interrupt, the counter transmits the count value of counting the number of pulses of the digital pulse type second frequency signal to the The ARM7 kernel is processed, and the ARM7 kernel calculates the concentration of the cyanomethemoglobin reaction solution to be measured according to two count values, and the ARM7 kernel stores the concentration of the cyanomethemoglobin reaction solution to be measured to the memory , while transmitting the concentration of the cyanmethemoglobin reaction solution to be tested to the display screen for display; 所述的稳定光源产生电路主要由稳压单元、控制单元和发光单元组成，所述的稳压单元包括稳压源、第一电解电容、第二电容、第三电解电容、第四电容和第一电阻，所述的控制单元包括第一三极管、第二三极管、第二电阻和第三电阻，所述的发光单元包括红色发光二极管和绿色发光二极管，所述的稳压源具有电压输入端、电压输出端和接地端，所述的稳压源的电压输入端接入工作电压，所述的稳压源的电压输入端分别与所述的第一电解电容的正极端和所述的第二电容的第一端相连接，所述的第一电解电容的负极端和所述的第二电容的第二端均接地，所述的稳压源的电压输出端分别与所述的第一电阻的第一端、所述的第三电解电容的正极端和所述的第四电容的第一端相连接，所述的稳压源的接地端分别与所述的第一电阻的第二端、所述的第三电解电容的负极端和所述的第四电容的第二端相连接，所述的ARM控制单元通过第一接线端子与所述的第二电阻的第一端相连接，所述的第二电阻的第二端与所述的第一三极管的基极相连接，所述的第一三极管的集电极与所述的红色发光二极管的负极端相连接，所述的ARM控制单元通过第二接线端子与所述的第三电阻的第一端相连接，所述的第三电阻的第二端与所述的第二三极管的基极相连接，所述的第二三极管的集电极与所述的绿色发光二极管的负极端相连接，所述的第一三极管的发射极和所述的第二三极管的发射极均接地，所述的红色发光二极管的正极端和所述的绿色发光二极管的正极端均与所述的稳压源的接地端相连接，所述的红色发光二极管发出的红光通过所述的比色皿的一侧壁入射到所述的比色皿内的待测氰化高铁血红蛋白反应溶液上，所述的绿色发光二极管发出的绿光通过所述的比色皿的一侧壁入射到所述的比色皿内的待测氰化高铁血红蛋白反应溶液上；The stable light source generating circuit is mainly composed of a voltage stabilizing unit, a control unit and a light emitting unit, and the voltage stabilizing unit includes a voltage stabilizing source, a first electrolytic capacitor, a second capacitor, a third electrolytic capacitor, a fourth capacitor and a A resistor, the control unit includes a first transistor, a second transistor, a second resistor and a third resistor, the light-emitting unit includes a red light-emitting diode and a green light-emitting diode, and the voltage stabilizing source has Voltage input terminal, voltage output terminal and ground terminal, the voltage input terminal of the voltage stabilizing source is connected to the working voltage, the voltage input terminal of the voltage stabilizing source is respectively connected to the positive terminal of the first electrolytic capacitor and the The first terminal of the second capacitor is connected, the negative terminal of the first electrolytic capacitor and the second terminal of the second capacitor are both grounded, and the voltage output terminals of the voltage stabilizing source are respectively connected to the The first terminal of the first resistor, the positive terminal of the third electrolytic capacitor and the first terminal of the fourth capacitor are connected, and the ground terminal of the voltage stabilizing source is respectively connected to the first terminal of the first resistor The second end of the third electrolytic capacitor, the negative end of the third electrolytic capacitor and the second end of the fourth capacitor are connected, and the ARM control unit is connected to the first terminal of the second resistor through the first connection terminal. The second end of the second resistor is connected to the base of the first triode, the collector of the first triode is connected to the negative end of the red light emitting diode The ARM control unit is connected to the first end of the third resistor through the second connection terminal, and the second end of the third resistor is connected to the base of the second triode connected, the collector of the second triode is connected to the negative terminal of the green light-emitting diode, the emitter of the first triode is connected to the emitter of the second triode Both are grounded, the positive end of the red light emitting diode and the positive end of the green light emitting diode are connected to the ground terminal of the voltage regulator source, and the red light emitted by the red light emitting diode passes through the The side wall of the cuvette is incident on the cyanmethemoglobin reaction solution to be measured in the cuvette, and the green light emitted by the green light-emitting diode is incident on the cuvette through the side wall of the cuvette. On the cyanmethemoglobin reaction solution to be tested in the described cuvette; 所述的稳压源采用型号为SPX1117的低功耗正向电压调节器，所述的低功耗正向电压调节器的电压输出端输出的电压为5V；所述的第一三极管和所述的第二三极管均采用NPN型三极管；The voltage stabilizing source adopts a low-power forward voltage regulator of SPX1117 model, and the output voltage of the voltage output terminal of the low-power forward voltage regulator is 5V; the first triode and The second triodes all adopt NPN type triodes; 所述的光电转换电路主要由光电二极管、第四电阻、第五电容、第一滑变器、第二滑变器和运算放大器组成，所述的光电二极管接收通过所述的比色皿内的待测氰化高铁血红蛋白溶液后的红色出射光和绿色出射光，所述的光电二极管的正极端接地，所述的光电二极管的正极端与所述的运算放大器的正相输入端相连接，所述的光电二极管的负极端分别与所述的第四电阻的第一端、所述的第五电容的第一端和所述的运算放大器的反相输入端相连接，所述的第四电阻的第二端与所述的第一滑变器的第一端相连接，所述的第一滑变器的第二端和所述的第一滑变器的滑片相连接，其公共连接端分别与所述的第五电容的第二端和所述的运算放大器的输出端相连接，所述的运算放大器的输出端与所述的A/D转换器的输入端相连接，所述的运算放大器的第一调零端与所述的第二滑变器的第一端相连接，所述的运算放大器的第二调零端与所述的第二滑变器的第二端相连接，所述的运算放大器的接正电源端与所述的第二滑变器的滑片相连接。The photoelectric conversion circuit is mainly composed of a photodiode, a fourth resistor, a fifth capacitor, a first slipper, a second slipper and an operational amplifier, and the photodiode receives the The red outgoing light and green outgoing light after the cyanide methemoglobin solution is to be measured, the positive end of the photodiode is grounded, and the positive end of the photodiode is connected to the positive phase input end of the operational amplifier, so The negative terminal of the photodiode is respectively connected with the first terminal of the fourth resistor, the first terminal of the fifth capacitor and the inverting input terminal of the operational amplifier, and the fourth resistor The second end of the first slip changer is connected to the first end of the first slip changer, the second end of the first slip changer is connected to the slider of the first slip changer, and the common connection Terminals are respectively connected with the second terminal of the fifth capacitor and the output terminal of the operational amplifier, the output terminal of the operational amplifier is connected with the input terminal of the A/D converter, the The first zero-adjustment terminal of the operational amplifier is connected to the first terminal of the second slipper, and the second zero-adjustment terminal of the operational amplifier is connected to the second terminal of the second slipper. connected, the positive power supply terminal of the operational amplifier is connected with the slider of the second slipper. 2.根据权利要求1所述的一种便携式血红蛋白溶液测量系统，其特征在于所述的第一接线端子接入高电平且所述的第二接线端子接入低电平时，所述的第一三极管处于饱和状态，所述的第二三极管处于截止状态，所述的红色发光二极管发出红光，所述的红色发光二极管发出的红光通过所述的比色皿的一侧壁入射到所述的比色皿内的待测氰化高铁血红蛋白反应溶液上；所述的第一接线端子接入低电平且所述的第二接线端子接入高电平时，所述的第一三极管处于截止状态，所述的第二三极管处于饱和状态，所述的绿色发光二极管发出绿光，所述的绿色发光二极管发出的绿光通过所述的比色皿的一侧壁入射到所述的比色皿内的待测氰化高铁血红蛋白反应溶液上。2. A portable hemoglobin solution measurement system according to claim 1, characterized in that when the first connection terminal is connected to a high level and the second connection terminal is connected to a low level, the second connection terminal is connected to a low level. One triode is in a saturated state, the second triode is in a cut-off state, the red light-emitting diode emits red light, and the red light emitted by the red light-emitting diode passes through one side of the cuvette The wall is incident on the cyanmethemoglobin reaction solution to be measured in the cuvette; when the first connection terminal is connected to a low level and the second connection terminal is connected to a high level, the The first triode is in a cut-off state, the second triode is in a saturated state, the green light-emitting diode emits green light, and the green light emitted by the green light-emitting diode passes through one of the cuvettes The side wall is incident on the cyanmethemoglobin reaction solution to be tested in the cuvette. 3.根据权利要求1所述的一种便携式血红蛋白溶液测量系统，其特征在于所述的运算放大器的型号为OP07或AD705。3. A portable hemoglobin solution measurement system according to claim 1, characterized in that the model of the operational amplifier is OP07 or AD705. 4.根据权利要求1所述的一种便携式血红蛋白溶液测量系统，其特征在于所述的A/D转换器采用电压-频率变换型A/D转换器。4. A portable hemoglobin solution measurement system according to claim 1, characterized in that said A/D converter adopts a voltage-frequency conversion type A/D converter. 5.一种权利要求1所述的便携式血红蛋白溶液测量系统相应的测量方法，其特征在于包括以下步骤：5. A corresponding measurement method of the portable hemoglobin solution measurement system according to claim 1, characterized in that it may further comprise the steps: ①初始化ARM控制单元，ARM控制单元内置的定时器被开启，其产生第一个定时中断，ARM控制单元的ARM7内核控制稳定光源产生电路的第一三极管使其处于饱和状态，第一三极管工作控制稳定光源产生电路的红色发光二极管发出具有稳定光强的红光；在此，ARM控制单元为基于ARM7内核的型号为S3C44B0X的微处理器；①Initialize the ARM control unit, the built-in timer of the ARM control unit is turned on, which generates the first timing interrupt, the ARM7 core of the ARM control unit controls the first triode of the stable light source generation circuit to make it in a saturated state, The red light-emitting diode of the stable light source generation circuit is controlled by the pole tube to emit red light with stable light intensity; here, the ARM control unit is a microprocessor based on the ARM7 core model S3C44B0X; ②红色发光二极管发出的红光入射到比色皿内的待测氰化高铁血红蛋白反应溶液上，待测氰化高铁血红蛋白反应溶液吸收部分红光，而通过待测氰化高铁血红蛋白反应溶液后的红色出射光照射到光电转换电路的光电二极管上，光电二极管将红色出射光的光强转换为第一模拟电压信号，光电转换电路的运算放大器对第一模拟电压信号进行放大处理，并将放大后的第一模拟电压信号传输给A/D转换器；②The red light emitted by the red light-emitting diode is incident on the cyanomethemoglobin reaction solution to be tested in the cuvette, and the cyanomethemoglobin reaction solution to be tested absorbs part of the red light, and the cyanomethemoglobin reaction solution to be tested The red outgoing light is irradiated on the photodiode of the photoelectric conversion circuit, and the photodiode converts the light intensity of the red outgoing light into a first analog voltage signal, and the operational amplifier of the photoelectric conversion circuit amplifies the first analog voltage signal, and converts the amplified The first analog voltage signal is transmitted to the A/D converter; ③A/D转换器将第一模拟电压信号转换为数字脉冲式的第一频率信号，并将数字脉冲式的第一频率信号传输给ARM控制单元的计数器，同时定时器产生第二个定时中断时，计数器对数字脉冲式的第一频率信号的脉冲个数进行计数；③The A/D converter converts the first analog voltage signal into a digital pulse-type first frequency signal, and transmits the digital pulse-type first frequency signal to the counter of the ARM control unit, and at the same time, the timer generates a second timing interrupt , the counter counts the number of pulses of the digital pulse type first frequency signal; ④定时器产生第三个定时中断时，计数器将其对数字脉冲式的第一频率信号的脉冲个数进行计数的计数值传输给ARM7内核进行处理，同时ARM7内核控制稳定光源产生电路的第二三极管使其处于饱和状态，第二三极管工作控制稳定光源产生电路的绿色发光二极管发出具有稳定光强的绿光；④When the timer generates the third timing interrupt, the counter transmits the count value of counting the number of pulses of the digital pulse type first frequency signal to the ARM7 core for processing, and at the same time the ARM7 core controls the second of the stable light source generating circuit The triode makes it in a saturated state, and the second triode works to control the green light-emitting diode of the stable light source generating circuit to emit green light with stable light intensity; ⑤绿色发光二极管发出的绿光入射到比色皿内的待测氰化高铁血红蛋白反应溶液上，待测氰化高铁血红蛋白反应溶液吸收部分绿光，而通过待测氰化高铁血红蛋白反应溶液后的绿色出射光照射到光电转换电路的光电二极管上，光电二极管将绿色出射光的光强转换为第二模拟电压信号，光电转换电路的运算放大器对第二模拟电压信号进行放大处理，并将放大后的第二模拟电压信号传输给A/D转换器；⑤ The green light emitted by the green light-emitting diode is incident on the cyanomethemoglobin reaction solution to be tested in the cuvette, the cyanomethemoglobin reaction solution to be tested absorbs part of the green light, and the cyanomethemoglobin reaction solution to be tested The green outgoing light is irradiated on the photodiode of the photoelectric conversion circuit, and the photodiode converts the light intensity of the green outgoing light into a second analog voltage signal, and the operational amplifier of the photoelectric conversion circuit amplifies the second analog voltage signal, and converts the amplified The second analog voltage signal is transmitted to the A/D converter; ⑥A/D转换器将第二模拟电压信号转换为数字脉冲式的第二频率信号，并将数字脉冲式的第二频率信号传输给ARM控制单元的计数器，同时定时器产生第四个定时中断时，计数器对数字脉冲式的第二频率信号的脉冲个数进行计数；⑥The A/D converter converts the second analog voltage signal into a digital pulse-type second frequency signal, and transmits the digital pulse-type second frequency signal to the counter of the ARM control unit, and at the same time, the timer generates the fourth timing interrupt , the counter counts the number of pulses of the digital pulse type second frequency signal; ⑦定时器产生第五个定时中断时，计数器将其对数字脉冲式的第二频率信号的脉冲个数进行计数的计数值传输给ARM7内核进行处理；⑦When the timer generates the fifth timing interrupt, the counter transmits the count value counting the number of pulses of the digital pulse type second frequency signal to the ARM7 core for processing; ⑧ARM7内核根据两个计数值计算得到待测氰化高铁血红蛋白反应溶液的浓度，记为C， C = 1 ( K 2 - K 1 ) L lg f 1 f 2 + 1 ( K 2 - K 1 ) L lg L in 2 L in 1 , 其中，K1表示红光的吸收系数，K2表示绿光的吸收系数，L表示比色皿的一侧壁的厚度，f1表示计数器对数字脉冲式的第一频率信号的脉冲个数进行计数得到的计数值，f2表示计数器对数字脉冲式的第二频率信号的脉冲个数进行计数得到的计数值，Lin1表示稳定光源产生电路的红色发光二极管发出的红光的光强，Lin2表示稳定光源产生电路的绿色发光二极管发出的绿光的光强。⑧The ARM7 kernel calculates the concentration of the cyanmethemoglobin reaction solution to be tested according to the two count values, which is denoted as C, C = 1 ( K 2 - K 1 ) L lg f 1 f 2 + 1 ( K 2 - K 1 ) L lg L in 2 L in 1 , Wherein, K 1 represents the absorption coefficient of red light, K 2 represents the absorption coefficient of green light, L represents the thickness of the side wall of the cuvette, and f 1 represents the number of pulses of the first frequency signal of the digital pulse type by the counter The count value obtained by counting, f2 represents the count value obtained by the counter counting the number of pulses of the digital pulse type second frequency signal, L in1 represents the light intensity of the red light emitted by the red light-emitting diode of the stable light source generating circuit, L in2 represents the light intensity of the green light emitted by the green light-emitting diode of the stable light source generating circuit. 6.根据权利要求5所述的一种便携式血红蛋白溶液测量系统相应的测量方法，其特征在于令
Figure FSB00000617122600042
为a，令
Figure FSB00000617122600043
为b，令
Figure FSB00000617122600044
为x，根据步骤①至⑧测量多组待测氰化高铁血红蛋白反应溶液对应的x和C，然后利用拟合方法拟合出a和b的具体值，最终得到待测氰化高铁血红蛋白反应溶液的浓度
Figure FSB00000617122600045
6. the corresponding measurement method of a kind of portable hemoglobin solution measurement system according to claim 5, is characterized in that order
Figure FSB00000617122600042
for a, let
Figure FSB00000617122600043
for b, let
Figure FSB00000617122600044
is x, according to steps ① to ⑧, measure x and C corresponding to multiple groups of cyanomethemoglobin reaction solutions to be tested, and then use the fitting method to fit the specific values of a and b, and finally obtain the cyanomethemoglobin reaction solution to be tested concentration
Figure FSB00000617122600045
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