CN103399006A - Color RGB (red, green and blue)-component-based urine analysis device and processing method thereof - Google Patents

Abstract

The invention discloses a urine analysis device based on color RGB components, which is characterized in that it includes a processor, a serial communication unit, a temperature measurement unit, a light intensity control unit and a color sensor unit, and the processor controls the white color through the light intensity control unit. The luminous intensity of the LED lamp, the light emitted by the white LED lamp is irradiated into the urine test strip on the sampling platform, and the color RGB component of the reflected light of each reagent area of the urine test strip is obtained by the color sensor unit, and the processor judges it according to the color RGB component and temperature The semi-quantitative value of the detection index corresponding to each reagent area is displayed. Its remarkable effect is: the circuit structure is simple, the cost is low, through the cooperation of the color sensor and the temperature detection unit, the empirical values of the existing precision instruments are used to fit the calculation coefficients of each detection index, and the microprocessor can use these coefficients to calculate Accurate detection index, high precision equipment, wide temperature range.

Description

Urine analysis device and processing method based on color RGB components

technical field

The invention relates to urine routine detection technology in the field of biomedicine, in particular to a urine analysis device based on color RGB components and a processing method thereof.

Background technique

Urinalysis is one of the most commonly used laboratory indicators in clinical practice. It not only provides references for the diagnosis and curative effect observation of urinary system diseases, but also is very helpful for the diagnosis and treatment of other system diseases that can cause changes in blood biochemical components. In addition, urinalysis can also be used for safe drug testing and initial assessment of health status.

As a traditional method, urine testing is often based on the color change produced by the reaction between the reagent area on the test strip and the biochemical components in the urine sample, qualitative or semi-quantitative detection of urobilinogen, bilirubin, and ketone bodies in the urine sample. , blood, protein, nitrite, white blood cells, glucose, vitamin C (or microalbumin) content and urine sample specific gravity, pH, etc.

With the improvement of people's living standards and the strengthening of health awareness, a large number of portable, low-cost, non-invasive testing medical instruments and equipment are gradually popularized in families, such as portable blood pressure monitors and portable blood glucose meters. With the development of color sensors and electronic technology, miniature household urine analyzers will enter the family in the near future as a means of people's own health evaluation.

Nowadays, the urine analyzer product market is ushering in a period of rapid development, and multiple items can be tested. But the key part of the urine analyzer is the optical system and the specific algorithm for qualitative or semi-quantitative analysis. Its principle is: irradiating light to the surface of the reaction zone of the test paper to generate reflected light, the intensity of the reflected light is related to the reaction color of each test item, and the content of various substances in the urine is analyzed by the reflected light. Existing high-precision urine analyzers usually have expensive equipment and complex structures. In order for urine analyzers to enter the home and meet the accuracy requirements, it is necessary to improve or update the existing urine analyzers.

Contents of the invention

In view of the deficiencies in the prior art, one of the purposes of the present invention is to propose a urine analysis device based on color RGB components in order to meet the portable needs of household medical instruments, and in order to make the detection accuracy of the portable instrument meet the requirements, the present invention also proposes A processing method of a urine analysis device based on color RGB components.

Its specific technical scheme is as follows:

A urine analysis device based on color RGB components, its key lies in: including a processor and a serial communication unit connected to the processor, a temperature measurement unit, a light intensity control unit and a color sensor unit, the processor controls the The unit controls the luminous intensity of the white LED lamp. The light emitted by the white LED lamp is irradiated into the urine test strip on the sampling platform, and the color sensor unit is used to obtain the color RGB components of the reflected light from each reagent area of the urine test strip. The semi-quantitative value of the detection index corresponding to each reagent area is determined by using the temperature information obtained by the temperature measurement unit.

According to the principle of drying analysis, when the reaction time is basically constant, the color change of each reagent area of the final test paper urine test strip is mainly related to the concentration and temperature of the corresponding components in the tested urine, so it is obtained through the color sensor unit. Each color component is also related to the concentration and temperature of the corresponding components in the tested urine. However, the reflectance is usually used to reflect the degree of color change in the reagent area, so the reflectance can be reflected by the color components obtained by the color sensor. In addition, there will be certain changes in the color of the tested urine with the same concentration of components measured at the same time. Therefore, to use the same reflectance to reflect the color change of the measured urine at the same concentration at different temperatures, the influence of temperature must be considered, that is, temperature should also be considered as a factor. Therefore, the value of the reflectance should be a quantitative relationship of four factors, namely the R, G, B components of the color and the temperature. Based on this, on the basis of the light intensity control unit ensuring the incident light intensity, this design uses the combination of the color sensor unit and the temperature measurement unit to effectively improve the accuracy of urine drying analysis and expand the temperature range of measurement.

In order to ensure circuit safety and prevent chip damage caused by excessive external voltage, a start-stop control unit is connected to the processor, and the start-stop control unit includes a diode Q2, and the collector of the diode Q2 is connected to a 3.3V power supply via a resistor R10 , the base of the diode Q2 is connected to the external control terminal through the resistor R11, the emitter of the diode Q2 is grounded, the base of the diode Q2 is also grounded through the resistor R12, and the power input terminal of the processor is connected to the collector of the diode Q2.

In order to ensure the light intensity of the incident light of the urine test strip, the light intensity control unit includes a dual integrated operational amplifier, the first pin of the dual integrated operational amplifier is connected to the base of the diode Q1 via a resistor R5, and the collector of the diode Q1 is connected to the base of the diode Q1 via The resistor R6 is connected to the power supply, the white LED light is connected between the emitter of the diode Q1 and the second pin of the dual integrated operational amplifier, the third pin of the dual integrated operational amplifier is connected to the seventh pin, and the dual integrated operational amplifier The 4th pin of the dual integrated op amp is connected to the PWM output of the processor through the resistor R2 and the resistor R1 in turn, one end of the resistor R2 is grounded through the capacitor C1, and the other end of the resistor R2 is grounded through the capacitor C2 , the 7th pin of the dual integrated operational amplifier is also grounded through the resistor R4 and the resistor R3 in turn, the 6th pin of the dual integrated operational amplifier is connected between the resistor R4 and the resistor R3, the 8th pin of the dual integrated operational amplifier is connected to the power supply Connected, the second pin of the dual integrated op amp is grounded through the sampling resistor R7.

In order to improve detection accuracy on the basis of reducing processor cost, the processor is an STM32F103RB micro-control chip, and a reference voltage unit is also connected to the processor.

Combined with the description of the above circuit structure, the processing method of a urine analysis device based on color RGB components proposed by the present invention is as follows:

Step 1: Select the color RGB components D _R , D _G , D _B of a certain reagent area in the urine test strip, where D _R is the red component, D _G is the green component, and D _B is the blue component;

Step 2: Calculate the reference reflectance y* ₂₅ according to equation (a), where c ₀ , c ₁ , c ₂ , c ₃ , c ₄ , c ₅ , and c ₆ are coefficients;

y* ₂₅ =c ₀ +c ₁ D _R +c ₂ (D _R ) ² +c ₃ D _G +c ₄ (D _G ) ² +c ₅ D _B +c ₆ (D _B ) ² (a);

Step 3: Calculate the actual reflectance y* according to equation (b), where c ₀ ', c ₁ ', c ₂ ', c ₃ ', and c ₄ ' are all coefficients, and t is the temperature measurement unit The detected ambient temperature, y ^* ₂₅ is the reference reflectance calculated in step 2;

y*＝c ₀ '+c ₁ 't+c ₂ 't ² +c ₃ 'y* ₂₅ +c ₄ '(y* ₂₅ ) ² (b);

Step 4: Determine the semi-quantitative value of the detection index corresponding to the reagent area according to the actual reflectance y* calculated in step 3 and the preset interval range.

As a further description, the coefficients in equation (a) and equation (b) are different for different reagent areas in the urine test strip, and the specific determination method is carried out according to the following steps:

Step 2-1: Select N temperature points within the range of 25±5°C, use the existing equipment to test the true reflectance of a reagent area in the urine test strip at each temperature point, and record it as y _25,m in turn, m=1 ~N, N is a positive integer greater than 4;

Step 2-2: Use the color sensor unit to obtain the detection parameters x _0,m ,x _1,m, x _2,m ,x _3,m ,x _4,m ,x _{5, m} ,x _6,m , where:

x _0,m = 1;

x _1,m ＝D _R,m , that is, the red component at the mth temperature point;

x _2,m ＝(D _R,m ) ² , that is, the square of the red component at the mth temperature point;

x _3,m ＝D _G,m , which is the green component at the mth temperature point;

x _4,m ＝(D _G,m ) ² , which is the square of the green component at the mth temperature point;

x _5,m = D _B,m , that is, the blue component at the mth temperature point;

x _6,m =(D _B,m ) ² is the square of the blue component at the mth temperature point;

Step 2-3: Determine the coefficients c ₀ , c ₁ , c ₂ , c ₃ , c ₄ , c ₅ , c ₆ in equation (a) according to the equation system shown in equation (c);

$\underset{\mathrm{<span\; class="notranslate">\; i</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 0</span>}}{\overset{\mathrm{<span\; class="notranslate">\; 6</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}{\mathrm{<span\; class="notranslate">\; c</span>}}_{\mathrm{<span\; class="notranslate">\; i</span>}}<span\; class="notranslate">\; (</span>\underset{\mathrm{<span\; class="notranslate">\; m</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 1</span>}}{\overset{\mathrm{<span\; class="notranslate">\; N</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; i</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; k</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}<span\; class="notranslate">\; )</span><span\; class="notranslate">\; =</span>\underset{\mathrm{<span\; class="notranslate">\; m</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 1</span>}}{\overset{\mathrm{<span\; class="notranslate">\; N</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}{\mathrm{<span\; class="notranslate">\; the\; y</span>}}_{\mathrm{<span\; class="notranslate">\; 25</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; k</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}<span\; class="notranslate">\; ,</span>}\mathrm{<span\; class="notranslate">\; k</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 1</span>}<span\; class="notranslate">\; ~</span>\mathrm{<span\; class="notranslate">\; 6</span>}<span\; class="notranslate">\; -</span><span\; class="notranslate">\; -</span><span\; class="notranslate">\; -</span><span\; class="notranslate">\; (</span>\mathrm{<span\; class="notranslate">\; c</span>}<span\; class="notranslate">\; )</span><span\; class="notranslate">\; ;</span>$

Step 2-4: Determine the coefficients c ₀ ', c ₁ ', c ₂ ', c ₃ ', c ₄ ' in equation (b) according to the equation system shown in equation (d);

in:

y _0,m = 1;

y _1,m =t _m , that is, the temperature value of the mth temperature point;

y _2,m = (t _m ) ² , that is, the square of the temperature value at the mth temperature point;

y _3,m =y* _25,m , which is the reference reflectance at the mth temperature point;

y _4,m =(y* _25,m ) ² , which is the square of the reference reflectance at the mth temperature point;

Reference reflectance at the mth temperature point:

y* _25,m ＝c ₀ +c ₁ D _R,m +c ₂ (D _R,m ) ² +c ₃ D _G,m +c ₄ (D _G,m ) ² +c ₅ D _B,m + c ₆ (D _B,m ) ² .

Using this method, the calculation coefficients of each detection index can be accurately confirmed, and these coefficients can be solidified into the microprocessor through the program, and the semi-quantitative value of each detection index can be directly calculated, reducing the calculation amount of the processor. The required cost is lower.

The remarkable effect of the present invention is: the circuit structure is simple, the cost is low, through the cooperation of the color sensor and the temperature detection unit, the empirical values of the existing precision instruments are used to fit the calculation coefficients of each detection index, and the microprocessor can use these coefficients Accurate detection indicators are calculated, and the equipment has high precision and a wide range of applicable temperatures, and can be widely used in family and clinical urine detection.

Description of drawings

Fig. 1 is a block diagram of circuit principle of the present invention;

Fig. 2 is a schematic circuit diagram of the start-stop control unit in Fig. 1;

FIG. 3 is a schematic circuit diagram of the light intensity control unit in FIG. 1 .

Detailed ways

The specific implementation manner and working principle of the present invention will be further described in detail below in conjunction with the accompanying drawings.

As shown in Figure 1, a urine analysis device based on color RGB components includes a processor 1 and a serial communication unit 3 connected to the processor 1, a temperature measurement unit 4, a light intensity control unit 5 and a color sensor unit 8. The processor 1 controls the luminous intensity of the white LED lamp 6 through the light intensity control unit 5, the light emitted by the white LED lamp 6 is irradiated into the urine test strip on the sampling platform, and the color sensor unit 8 is used to obtain each reagent area of the urine test strip The color RGB components of the reflected light, the processor 1 determines the semi-quantitative value of each reagent area corresponding to the detection index according to the color RGB components and the temperature information acquired by the temperature measurement unit 4 .

During the implementation process, processor 1 chooses a cost-effective 32-bit microprocessor, the model is STM32F103RB, with a 12-bit, 1μs conversion speed A/D converter, 128K flash memory for program and data storage, and 20K static Storage, the highest frequency can reach 72MHz, the temperature measurement unit 4 usually uses a 18B20 temperature sensor, and the color sensor unit 8 uses a TCS3104 color sensor. On the one hand, the processor 1 controls the luminous intensity of the white LED lamp 6 through the light intensity control unit 5 to ensure the stability of the incident light intensity. On the other hand, the processor 1 is used to convert the analog voltage output by the color sensor unit 8 and the temperature measurement unit 4 into corresponding digital values to realize the collection of color and temperature, and calculate the semi-quantitative value of each index through an internal algorithm, At the same time, the serial communication unit 3 is used to communicate with the outside.

Considering that the power supply of the analog input may fluctuate, in order to meet the consistency between different modules, a reference voltage unit 7 is also connected to the processor 1. The reference voltage unit 7 selects a high-precision 3V reference voltage chip ADR363B, and the output voltage noise The peak-to-peak value is only 6.8V, the voltage accuracy is ±3mV, and the temperature coefficient is only 9ppm/℃.

As shown in Figure 2, in order to ensure the safety of the power supply of the processor 1, the processor 1 is connected with a start-stop control unit 2, the start-stop control unit 2 includes a diode Q2, the collector of the diode Q2 is connected to the resistor R10 and 3.3 V power supply is connected, the base of diode Q2 is connected to the external control terminal through resistor R11, the emitter of diode Q2 is grounded, the base of diode Q2 is also grounded through resistor R12, and the power input terminal of processor 1 is connected to the collector of diode Q2 superior.

As shown in Figure 3, as an implementation, the light intensity control unit 5 includes a dual integrated operational amplifier AMP2, the first pin of the dual integrated operational amplifier AMP2 is connected to the base of the diode Q1 through a resistor R5, and the diode Q1 The collector of the diode Q1 is connected to the power supply through the resistor R6, the white LED light 6 is connected between the emitter of the diode Q1 and the second pin of the dual integrated operational amplifier AMP2, and the third pin of the dual integrated operational amplifier AMP2 is connected to the seventh tube The pins are connected, the fourth pin of the dual integrated operational amplifier AMP2 is grounded, the fifth pin of the dual integrated operational amplifier AMP2 is connected to the PWM output of processor 1 through the resistor R2 and the resistor R1 in turn, and one end of the resistor R2 is grounded through the capacitor C1 , the other end of the resistor R2 is grounded through the capacitor C2, the seventh pin of the dual integrated operational amplifier AMP2 is also grounded through the resistor R4 and the resistor R3 in turn, and the sixth pin of the dual integrated operational amplifier AMP2 is connected between the resistor R4 and the resistor R3 , the eighth pin of the dual integrated operational amplifier AMP2 is connected to the power supply, and the second pin of the dual integrated operational amplifier AMP2 is grounded through the sampling resistor R7.

Resistor R1, resistor R2, capacitor C1 and capacitor C2 form a filter to filter the pulse width modulation signal (PWM) output by processor 1 into a DC signal; resistor R3, resistor R4 and an integrated operational amplifier in the dual integrated operational amplifier AMP2 Constitute the same-phase proportional amplifier to achieve the purpose of amplifying the DC signal after the PWM signal is filtered; resistor R5, resistor R6, sampling resistor R7, triode Q1 and an integrated operational amplifier in the dual integrated operational amplifier AMP2 realize voltage-current conversion to achieve constant Flow control white LED light 6 purpose.

According to the above equipment, taking the detection of protein (PRO) as an example, the processing method of this urine analysis device based on color RGB components is described in detail, and the processing steps are as follows:

In order to detect multiple indicators, urine test strips usually have multiple reagent areas. The detection reagents added to different reagent areas are different, and their color components and corresponding reflectances are also different. For protein detection:

First enter step 1: select the color RGB components _DR , D _G , and _DB of the reagent area for detecting protein in the urine test strip, where _DR is the red component, D _G is the green component, and _DB is the blue component;

Then go to step 2: Calculate the reference reflectance y* ₂₅ according to equation (a). In equation (a), c ₀ , c ₁ , c ₂ , c ₃ , c ₄ , c ₅ , and c ₆ are all coefficients. Here should correspond to the coefficient of the detected protein;

y* ₂₅ =c ₀ +c ₁ D _R +c ₂ (D _R ) ² +c ₃ D _G +c ₄ (D _G ) ² +c ₅ D _B +c ₆ (D _B ) ² (a);

When the reference reflectance y* ₂₅ is calculated, enter step 3: calculate the actual reflectance y* according to equation (b), in equation (b) c ₀ ', c ₁ ', c ₂ ', c ₃ ', c ₄ ' are coefficients, the coefficients here are also coefficients for detecting proteins, t is the ambient temperature detected by the temperature measurement unit 4, and y ^* ₂₅ is the calculated reference reflectance in step 2;

y*＝c ₀ '+c ₁ 't+c ₂ 't ² +c ₃ 'y* ₂₅ +c ₄ '(y* ₂₅ ) ² (b);

After the actual reflectance y* is calculated according to the above coefficients, proceed to step 4: determine the semi-quantitative value of the detection index corresponding to the reagent area according to the size of the actual reflectance y* calculated in step 3 and the preset interval range.

In the specific implementation process, the relationship between the concentration range, reflectance and semi-quantitative value of protein (PRO) is shown in Table 1.

Table 1 The relationship between the concentration interval, reflectance and semi-quantitative value of white matter (PRO)

In the above method, the coefficients in equation (a) and equation (b) are different for different reagent areas in the urine test strip. Here, for the concentration detection of protein (PRO), the determination method of each coefficient is specifically introduced.

Step 2-1: Select N temperature points within the range of 25±5°C. Here, 5 temperature points of 21°C, 23°C, 25°C, 27°C, and 29°C are selected for testing, and each temperature is tested with existing instruments Click on the true reflectance of a reagent area in the urine test strip, which is recorded as y _25,m in turn, m=1～N, where N=5, and the existing instrument selected is Bayer Telit-Ainas CLINITEKATLAS, Germany. Measurement;

Step 2-2: Use the color sensor unit 8 to obtain detection parameters x _0,m ,x _1,m ,x _2,m ,x _3,m ,x _4,m ,x ₅ of the reagent zone at each temperature point _,m ,x _6,m , where:

x _0,m = 1;

x _1,m ＝D _R,m , that is, the red component at the mth temperature point;

x _2,m ＝(D _R,m ) ² , that is, the square of the red component at the mth temperature point;

x _3,m ＝D _G,m , which is the green component at the mth temperature point;

x _4,m ＝(D _G,m ) ² , which is the square of the green component at the mth temperature point;

x _5,m = D _B,m , that is, the blue component at the mth temperature point;

x _6,m =(D _B,m ) ² is the square of the blue component at the mth temperature point;

Step 2-3: Determine the coefficients c ₀ , c ₁ , c ₂ , c ₃ , c ₄ , c ₅ , c ₆ in equation (a) according to the equation system shown in equation (c);

$\underset{\mathrm{<span\; class="notranslate">\; i</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 0</span>}}{\overset{\mathrm{<span\; class="notranslate">\; 6</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}{\mathrm{<span\; class="notranslate">\; c</span>}}_{\mathrm{<span\; class="notranslate">\; i</span>}}<span\; class="notranslate">\; (</span>\underset{\mathrm{<span\; class="notranslate">\; m</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 1</span>}}{\overset{\mathrm{<span\; class="notranslate">\; N</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; i</span>}<span\; class="notranslate">\; .</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; k</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}<span\; class="notranslate">\; )</span><span\; class="notranslate">\; =</span>\underset{\mathrm{<span\; class="notranslate">\; m</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 1</span>}}{\overset{\mathrm{<span\; class="notranslate">\; N</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}{\mathrm{<span\; class="notranslate">\; the\; y</span>}}_{\mathrm{<span\; class="notranslate">\; 25</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; k</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; k</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 1</span>}<span\; class="notranslate">\; ~</span>\mathrm{<span\; class="notranslate">\; 6</span>}<span\; class="notranslate">\; -</span><span\; class="notranslate">\; -</span><span\; class="notranslate">\; -</span><span\; class="notranslate">\; (</span>\mathrm{<span\; class="notranslate">\; c</span>}<span\; class="notranslate">\; )</span><span\; class="notranslate">\; ;</span>$

Step 2-4: Determine the coefficients c ₀ ', c ₁ ', c ₂ ', c ₃ ', c ₄ ' in equation (b) according to the equation system shown in equation (d);

$\underset{\mathrm{<span\; class="notranslate">\; j</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 0</span>}}{\overset{\mathrm{<span\; class="notranslate">\; 6</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}{\mathrm{<span\; class="notranslate">\; c</span>}}_{\mathrm{<span\; class="notranslate">\; j</span>}}<span\; class="notranslate">\; \&prime;</span><span\; class="notranslate">\; (</span>\underset{\mathrm{<span\; class="notranslate">\; m</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 1</span>}}{\overset{\mathrm{<span\; class="notranslate">\; N</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}{\mathrm{<span\; class="notranslate">\; the\; y</span>}}_{\mathrm{<span\; class="notranslate">\; j</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; the\; y</span>}}_{\mathrm{<span\; class="notranslate">\; l</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}<span\; class="notranslate">\; )</span><span\; class="notranslate">\; =</span>\underset{\mathrm{<span\; class="notranslate">\; m</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 1</span>}}{\overset{\mathrm{<span\; class="notranslate">\; N</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}{\mathrm{<span\; class="notranslate">\; the\; y</span>}}_{\mathrm{<span\; class="notranslate">\; 25</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; the\; y</span>}}_{\mathrm{<span\; class="notranslate">\; l</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; l</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 1</span>}<span\; class="notranslate">\; ~</span>\mathrm{<span\; class="notranslate">\; 4</span>}<span\; class="notranslate">\; -</span><span\; class="notranslate">\; -</span><span\; class="notranslate">\; -</span><span\; class="notranslate">\; (</span>\mathrm{<span\; class="notranslate">\; d</span>}<span\; class="notranslate">\; )</span><span\; class="notranslate">\; ;</span>$

in:

y _0,m = 1;

y _1,m =t _m , that is, the temperature value of the mth temperature point;

y _2,m = (t _m ) ² , that is, the square of the temperature value at the mth temperature point;

y _3,m =y* _25,m , which is the reference reflectance at the mth temperature point;

y _4,m =(y* _25,m ) ² , which is the square of the reference reflectance at the mth temperature point;

Reference reflectance at the mth temperature point:

y* _25,m ＝c ₀ +c ₁ D _R,m +c ₂ (D _R,m ) ² +c ₃ D _G,m +c ₄ (D _G,m ) ² +c ₅ D _B,m + c ₆ (D _B,m ) ² .

According to the above method, after determining the corresponding coefficients, the equation (a) is:

y ₂₅ *＝-118.6566+0.2213D _R +0.0014(D _R ) ² +1.7419D _G

-0.0056(D _G ) ² -0.0398D _B -0.0002(D _B ) ²

Equation (b) is:

y*=-8.8512+0.4278t-0.0024t ² +1.0034y ₂₅ *-0.0001(y ₂₅ *) ² .

In the process of determining each coefficient, the principle of least squares multiple regression is mainly used, and y _25,m is used to represent the reflectance of the mth temperature point actually measured, and y* _25,m is the fitting of the mth temperature point , then its difference δ _25,m = |y _25,m -y* _25,m |, according to the principle of the least square method to make $\underset{m=1}{\overset{N}{\mathrm{\&Sigma;}}}{{\mathrm{\&delta;}}_{25,\mathrm{<figure-callout\; id="2"\; label="m"\; filenames="HDA00003581075500011.png,HDA00003581075500021.png"\; state="\{\{state\}\}">m</figure-callout>}}}^2=\underset{m=1}{\overset{N}{\mathrm{\&Sigma;}}}{({\mathrm{the\; y}}_{25,m}-{\mathrm{the\; y}*}_{25,m})}^2$ minimum, that is:

$\underset{m=1}{\overset{N}{\mathrm{\&Sigma;}}}{({\mathrm{the\; y}}_{25,m}-{\mathrm{the\; y}*}_{25,m})}^2=\underset{m=1}{\overset{N}{\mathrm{\&Sigma;}}}{({\mathrm{the\; y}}_{25.m}-\underset{i=0}{\overset{6}{\mathrm{\&Sigma;}}}{c}_i{x}_{i.m})}^2$ minimum, then:

$\frac{\&PartialD;\left[\underset{m=1}{\overset{N}{\mathrm{\&Sigma;}}}{({\mathrm{the\; y}}_m-\underset{i=0}{\overset{6}{\mathrm{\&Sigma;}}}{c}_i{x}_{i,m})}^2\right]}{\&PartialD;c_k}=0(k=0,...,6)\text{'}$ Right now:

$-\underset{m=1}{\overset{N}{\mathrm{\&Sigma;}}}2({\mathrm{the\; y}}_{25,m}-\underset{i=0}{\overset{6}{\mathrm{\&Sigma;}}}{c}_i{x}_{i,m})x_{k,m}=0;$ After finishing there are:

$\underset{m=1}{\overset{N}{\mathrm{\&Sigma;}}}\left(\underset{i=0}{\overset{6}{\mathrm{\&Sigma;}}}{c}_i{x}_{i,m}\right)x_{k,m}=\underset{m=1}{\overset{N}{\mathrm{\&Sigma;}}}{\mathrm{the\; y}}_{25,m}{x}_{k,m};$ where (k=0,...,6), because:

$\underset{\mathrm{<span\; class="notranslate">\; m</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 1</span>}}{\overset{\mathrm{<span\; class="notranslate">\; N</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}<span\; class="notranslate">\; (</span>\underset{\mathrm{<span\; class="notranslate">\; i</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 0</span>}}{\overset{\mathrm{<span\; class="notranslate">\; 6</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}{\mathrm{<span\; class="notranslate">\; c</span>}}_{\mathrm{<span\; class="notranslate">\; i</span>}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; i</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}<span\; class="notranslate">\; )</span>{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; k</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}<span\; class="notranslate">\; =</span>\underset{\mathrm{<span\; class="notranslate">\; m</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 1</span>}}{\overset{\mathrm{<span\; class="notranslate">\; N</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}<span\; class="notranslate">\; (</span>\underset{\mathrm{<span\; class="notranslate">\; i</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 0</span>}}{\overset{\mathrm{<span\; class="notranslate">\; 6</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}{\mathrm{<span\; class="notranslate">\; c</span>}}_{\mathrm{<span\; class="notranslate">\; i</span>}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; i</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; k</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}<span\; class="notranslate">\; )</span><span\; class="notranslate">\; =</span>\underset{\mathrm{<span\; class="notranslate">\; i</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 0</span>}}{\overset{\mathrm{<span\; class="notranslate">\; 6</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}<span\; class="notranslate">\; (</span>\underset{\mathrm{<span\; class="notranslate">\; m</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 1</span>}}{\overset{\mathrm{<span\; class="notranslate">\; N</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}{\mathrm{<span\; class="notranslate">\; c</span>}}_{\mathrm{<span\; class="notranslate">\; i</span>}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; i</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; k</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}<span\; class="notranslate">\; )</span><span\; class="notranslate">\; =</span>\underset{\mathrm{<span\; class="notranslate">\; i</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 0</span>}}{\overset{\mathrm{<span\; class="notranslate">\; 6</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}{\mathrm{<span\; class="notranslate">\; c</span>}}_{\mathrm{<span\; class="notranslate">\; i</span>}}<span\; class="notranslate">\; (</span>\underset{\mathrm{<span\; class="notranslate">\; m</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 1</span>}}{\overset{\mathrm{<span\; class="notranslate">\; N</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; i</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; k</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}<span\; class="notranslate">\; )</span>$

So it can be organized as:

$\underset{i=0}{\overset{6}{\mathrm{\&Sigma;}}}{c}_{\text{i}}\left(\underset{m=1}{\overset{N}{\mathrm{\&Sigma;}}}{x}_{i,m}{x}_{k,m}\right)=\underset{m=1}{\overset{N}{\mathrm{\&Sigma;}}}{\mathrm{the\; y}}_{25,m}{x}_{k,m};$ Wherein (k=0,...,6), can also be written as the following 7 equations:

$\left\{\begin{array}{c}\underset{\mathrm{<span\; class="notranslate">\; m</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 1</span>}}{\overset{\mathrm{<span\; class="notranslate">\; N</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 0</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 0</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; c</span>}}_{\mathrm{<span\; class="notranslate">\; 0</span>}}<span\; class="notranslate">\; +</span>\underset{\mathrm{<span\; class="notranslate">\; m</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 1</span>}}{\overset{\mathrm{<span\; class="notranslate">\; N</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 1</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 0</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; c</span>}}_{\mathrm{<span\; class="notranslate">\; 1</span>}}<span\; class="notranslate">\; +</span>\underset{\mathrm{<span\; class="notranslate">\; m</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 1</span>}}{\overset{\mathrm{<span\; class="notranslate">\; N</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 2</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 0</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; c</span>}}_{\mathrm{<span\; class="notranslate">\; 2</span>}}<span\; class="notranslate">\; +</span>\underset{\mathrm{<span\; class="notranslate">\; m</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 1</span>}}{\overset{\mathrm{<span\; class="notranslate">\; N</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 3</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 0</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; c</span>}}_{\mathrm{<span\; class="notranslate">\; 3</span>}}<span\; class="notranslate">\; +</span>\underset{\mathrm{<span\; class="notranslate">\; m</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 1</span>}}{\overset{\mathrm{<span\; class="notranslate">\; N</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 4</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 0</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; c</span>}}_{\mathrm{<span\; class="notranslate">\; 4</span>}}\\ <span\; class="notranslate">\; +</span>\underset{\mathrm{<span\; class="notranslate">\; m</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 1</span>}}{\overset{\mathrm{<span\; class="notranslate">\; N</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 5</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 0</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; c</span>}}_{\mathrm{<span\; class="notranslate">\; 5</span>}}<span\; class="notranslate">\; +</span>\underset{\mathrm{<span\; class="notranslate">\; m</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 1</span>}}{\overset{\mathrm{<span\; class="notranslate">\; N</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 6</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 0</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; c</span>}}_{\mathrm{<span\; class="notranslate">\; 6</span>}}<span\; class="notranslate">\; =</span>\underset{\mathrm{<span\; class="notranslate">\; m</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 1</span>}}{\overset{\mathrm{<span\; class="notranslate">\; N</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}{\mathrm{<span\; class="notranslate">\; the\; y</span>}}_{\mathrm{<span\; class="notranslate">\; 25</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 0</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}\\ \underset{\mathrm{<span\; class="notranslate">\; m</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 1</span>}}{\overset{\mathrm{<span\; class="notranslate">\; N</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 0</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 1</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; c</span>}}_{\mathrm{<span\; class="notranslate">\; 0</span>}}<span\; class="notranslate">\; +</span>\underset{\mathrm{<span\; class="notranslate">\; m</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 1</span>}}{\overset{\mathrm{<span\; class="notranslate">\; N</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 1</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 1</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; c</span>}}_{\mathrm{<span\; class="notranslate">\; 1</span>}}<span\; class="notranslate">\; +</span>\underset{\mathrm{<span\; class="notranslate">\; m</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 1</span>}}{\overset{\mathrm{<span\; class="notranslate">\; N</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 2</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 1</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; c</span>}}_{\mathrm{<span\; class="notranslate">\; 2</span>}}<span\; class="notranslate">\; +</span>\underset{\mathrm{<span\; class="notranslate">\; m</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 1</span>}}{\overset{\mathrm{<span\; class="notranslate">\; N</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 3</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 1</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; c</span>}}_{\mathrm{<span\; class="notranslate">\; 3</span>}}<span\; class="notranslate">\; +</span>\underset{\mathrm{<span\; class="notranslate">\; m</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 1</span>}}{\overset{\mathrm{<span\; class="notranslate">\; N</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 4</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 1</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; c</span>}}_{\mathrm{<span\; class="notranslate">\; 4</span>}}\\ <span\; class="notranslate">\; +</span>\underset{\mathrm{<span\; class="notranslate">\; m</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 1</span>}}{\overset{\mathrm{<span\; class="notranslate">\; N</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 5</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 1</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; c</span>}}_{\mathrm{<span\; class="notranslate">\; 5</span>}}<span\; class="notranslate">\; +</span>\underset{\mathrm{<span\; class="notranslate">\; m</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 1</span>}}{\overset{\mathrm{<span\; class="notranslate">\; N</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 6</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 1</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; c</span>}}_{\mathrm{<span\; class="notranslate">\; 6</span>}}<span\; class="notranslate">\; =</span>\underset{\mathrm{<span\; class="notranslate">\; m</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 1</span>}}{\overset{\mathrm{<span\; class="notranslate">\; N</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}{\mathrm{<span\; class="notranslate">\; the\; y</span>}}_{\mathrm{<span\; class="notranslate">\; 25</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 1</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}\\ <span\; class="notranslate">\; .</span><span\; class="notranslate">\; .</span><span\; class="notranslate">\; .</span>\\ <span\; class="notranslate">\; .</span><span\; class="notranslate">\; .</span><span\; class="notranslate">\; .</span>\\ <span\; class="notranslate">\; .</span><span\; class="notranslate">\; .</span><span\; class="notranslate">\; .</span>\\ \underset{\mathrm{<span\; class="notranslate">\; m</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 1</span>}}{\overset{\mathrm{<span\; class="notranslate">\; N</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 0</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 6</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; c</span>}}_{\mathrm{<span\; class="notranslate">\; 0</span>}}<span\; class="notranslate">\; +</span>\underset{\mathrm{<span\; class="notranslate">\; m</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 1</span>}}{\overset{\mathrm{<span\; class="notranslate">\; N</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 1</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 6</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; c</span>}}_{\mathrm{<span\; class="notranslate">\; 1</span>}}<span\; class="notranslate">\; +</span>\underset{\mathrm{<span\; class="notranslate">\; m</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 1</span>}}{\overset{\mathrm{<span\; class="notranslate">\; N</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 2</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 6</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; c</span>}}_{\mathrm{<span\; class="notranslate">\; 2</span>}}<span\; class="notranslate">\; +</span>\underset{\mathrm{<span\; class="notranslate">\; m</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 1</span>}}{\overset{\mathrm{<span\; class="notranslate">\; N</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 3</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 6</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; c</span>}}_{\mathrm{<span\; class="notranslate">\; 3</span>}}<span\; class="notranslate">\; +</span>\underset{\mathrm{<span\; class="notranslate">\; m</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 1</span>}}{\overset{\mathrm{<span\; class="notranslate">\; N</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 4</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 6</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; c</span>}}_{\mathrm{<span\; class="notranslate">\; 4</span>}}\\ <span\; class="notranslate">\; +</span>\underset{\mathrm{<span\; class="notranslate">\; m</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 1</span>}}{\overset{\mathrm{<span\; class="notranslate">\; N</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 5</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 6</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; c</span>}}_{\mathrm{<span\; class="notranslate">\; 5</span>}}<span\; class="notranslate">\; +</span>\underset{\mathrm{<span\; class="notranslate">\; m</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 1</span>}}{\overset{\mathrm{<span\; class="notranslate">\; N</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 6</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 6</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; c</span>}}_{\mathrm{<span\; class="notranslate">\; 6</span>}}<span\; class="notranslate">\; =</span>\underset{\mathrm{<span\; class="notranslate">\; m</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 1</span>}}{\overset{\mathrm{<span\; class="notranslate">\; N</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}{\mathrm{<span\; class="notranslate">\; the\; y</span>}}_{\mathrm{<span\; class="notranslate">\; 25</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 6</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}\end{array}\right.$

Put the measured data of each item into the above 7 equations, and solve c ₀ ,c ₁ ,…,c ₆ , then the multi-variable fitting function can be obtained

y ₂₅ *＝c ₀ x ₀ +c ₁ x ₁ +c ₂ x ₂ +c ₃ x ₃ +c ₄ x ₄ +c ₅ x ₅ +c ₆ x ₆

In the same way, each coefficient in y*=c ₀ '+c ₁ 't+c ₂ 't ² +c ₃ 'y* ₂₅ +c ₄ '(y* ₂₅ ) ² can be fitted, and finally you can follow these The coefficients and corresponding detection parameters are used to calculate the actual reflectance.

The reflectance calculated by the above method is compared with the result tested by the high-end instrument Telit-Ainas CLINITEK ATLAS, as shown in Table 2, it can be seen that under different concentrations and different temperatures, the measured reflectance of the present invention The obtained reflectance has a small error with the reflectance measured by existing high-end instruments, and it can be guaranteed to be consistent after being converted into a semi-quantitative value, which meets the needs of household and even clinical use, and greatly reduces the cost of equipment.

This example only takes the detection of protein as an example to describe its implementation process in detail. For other indicators in routine urine detection, such as urobilinogen, bilirubin, ketone bodies, nitrite, white blood cells, glucose, etc. , the processing process and principle are the same, and will not be described again.

Table 2: Comparison table of reflectance test of protein under different concentration gradients and different temperatures

Claims (6)

1. A urine analysis device based on color RGB components, characterized in that it includes a processor (1) and a serial communication unit (3) connected to the processor (1), a temperature measurement unit (4), a light The intensity control unit (5) and the color sensor unit (8), the processor (1) controls the luminous intensity of the white LED lamp (6) through the light intensity control unit (5), and the light emitted by the white LED lamp (6) irradiates the sampler In the urine test strip on the platform, the color sensor unit (8) is used to obtain the color RGB components of the reflected light of each reagent area of the urine test strip, and the processor (1) judges according to the color RGB components and the temperature information obtained by the temperature measurement unit (4) The semi-quantitative value of the detection index corresponding to each reagent area is displayed. 2. The urine analysis device based on color RGB components according to claim 1, characterized in that a start-stop control unit (2) is connected to the processor (1), and the start-stop control unit (2) includes Diode Q2, the collector of the diode Q2 is connected to the 3.3V power supply through the resistor R10, the base of the diode Q2 is connected to the external control terminal through the resistor R11, the emitter of the diode Q2 is grounded, and the base of the diode Q2 is also grounded through the resistor R12, The power input terminal of the processor (1) is connected to the collector of the diode Q2. 3. The urine analysis device based on color RGB components according to claim 1, characterized in that: the light intensity control unit (5) includes a dual integrated operational amplifier (AMP2), and the dual integrated operational amplifier (AMP2) The first pin is connected to the base of the diode Q1 through the resistor R5, the collector of the diode Q1 is connected to the power supply through the resistor R6, and the emitter of the diode Q1 is connected to the second pin of the dual integrated operational amplifier (AMP2). White LED light (6), the 3rd pin of the dual integrated operational amplifier (AMP2) is connected to the 7th pin, the 4th pin of the dual integrated operational amplifier (AMP2) is grounded, the 5th pin of the dual integrated operational amplifier (AMP2) The pins are connected to the PWM output terminal of the processor (1) through the resistor R2 and the resistor R1 in sequence. One end of the resistor R2 is grounded through the capacitor C1, and the other end of the resistor R2 is grounded through the capacitor C2. The pins are also grounded through resistor R4 and resistor R3 in turn. The sixth pin of the dual integrated operational amplifier (AMP2) is connected between the resistor R4 and the resistor R3, and the eighth pin of the dual integrated operational amplifier (AMP2) is connected to the power supply. The second pin of the dual integrated operational amplifier (AMP2) is also grounded through the sampling resistor R7. 4. The urine analysis device based on color RGB components according to any one of claims 1-3, characterized in that: the processor (1) is a STM32F103RB micro-control chip, and the processor (1) is also A reference voltage unit (7) is connected. 5. a processing method based on the urine analysis device of color RGB component as claimed in claim 1, is characterized in that carrying out according to the following steps: Step 1: Select the color RGB components D _R , D _G , D _B of a certain reagent area in the urine test strip, where D _R is the red component, D _G is the green component, and D _B is the blue component; Step 2: Calculate the reference reflectance y* ₂₅ according to equation (a), where c ₀ , c ₁ , c ₂ , c ₃ , c ₄ , c ₅ , and c ₆ are coefficients; y* ₂₅ =c ₀ +c ₁ D _R +c ₂ (D _R ) ² +c ₃ D _G +c ₄ (D _G ) ² +c ₅ D _B +c ₆ (D _B ) ² (a); Step 3: Calculate the actual reflectance y* according to equation (b), where c ₀ ', c ₁ ', c ₂ ', c ₃ ', and c ₄ ' are all coefficients, and t is the temperature measurement unit (4) The detected ambient temperature, y ^* ₂₅ is the reference reflectance calculated in step 2; y*＝c ₀ '+c ₁ 't+c ₂ 't ² +c ₃ 'y* ₂₅ +c ₄ '(y* ₂₅ ) ² (b); Step 4: Determine the semi-quantitative value of the detection index corresponding to the reagent area according to the actual reflectance y* calculated in step 3 and the preset interval range. 6. The processing method of a urine analysis device based on color RGB components according to claim 5, characterized in that: the coefficients in equation (a) and equation (b) have different reagent areas in the urine test strip. The specific determination method is carried out according to the following steps: Step 2-1: Select N temperature points within the range of 25±5°C, use the existing equipment to test the true reflectance of a reagent area in the urine test strip at each temperature point, and record it as y _25,m in turn, m=1 ~N, N is a positive integer greater than 4; Step 2-2: Use the color sensor unit (8) to obtain detection parameters x _0,m ,x _1,m ,x _2,m ,x _3,m ,x _4,m , x _5,m ,x _6,m , where: x _0,m = 1; x _1,m ＝D _R,m , that is, the red component at the mth temperature point; x _2,m ＝(D _R,m ) ² , that is, the square of the red component at the mth temperature point; x _3,m ＝D _G,m , which is the green component at the mth temperature point; x _4,m ＝(D _G,m ) ² , which is the square of the green component at the mth temperature point; x _5,m = D _B,m , that is, the blue component at the mth temperature point; x _6,m =(D _B,m ) ² is the square of the blue component at the mth temperature point; Step 2-3: Determine the coefficients c ₀ , c ₁ , c ₂ , c ₃ , c ₄ , c ₅ , c ₆ in equation (a) according to the equation system shown in equation (c); $\underset{\mathrm{<span\; class="notranslate">\; i</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 0</span>}}{\overset{\mathrm{<span\; class="notranslate">\; 6</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}{\mathrm{<span\; class="notranslate">\; c</span>}}_{\mathrm{<span\; class="notranslate">\; i</span>}}<span\; class="notranslate">\; (</span>\underset{\mathrm{<span\; class="notranslate">\; m</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 1</span>}}{\overset{\mathrm{<span\; class="notranslate">\; N</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; i</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; k</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}<span\; class="notranslate">\; )</span><span\; class="notranslate">\; =</span>\underset{\mathrm{<span\; class="notranslate">\; m</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 1</span>}}{\overset{\mathrm{<span\; class="notranslate">\; N</span>}}{\mathrm{<span\; class="notranslate">\; \&Sigma;</span>}}}{\mathrm{<span\; class="notranslate">\; the\; y</span>}}_{\mathrm{<span\; class="notranslate">\; 25</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}{\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; k</span>}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; m</span>}}<span\; class="notranslate">\; ,</span>\mathrm{<span\; class="notranslate">\; k</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 1</span>}<span\; class="notranslate">\; ~</span>\mathrm{<span\; class="notranslate">\; 6</span>}<span\; class="notranslate">\; -</span><span\; class="notranslate">\; -</span><span\; class="notranslate">\; -</span><span\; class="notranslate">\; (</span>\mathrm{<span\; class="notranslate">\; c</span>}<span\; class="notranslate">\; )</span><span\; class="notranslate">\; ;</span>$ Step 2-4: Determine the coefficients c ₀ ', c ₁ ', c ₂ ', c ₃ ', c ₄ ' in equation (b) according to the equation system shown in equation (d); in: y _0,m = 1; y _1,m =t _m , that is, the temperature value of the mth temperature point; y _2,m = (t _m ) ² , that is, the square of the temperature value at the mth temperature point; y _3,m =y* _25,m , which is the reference reflectance at the mth temperature point; y _4,m =(y* _25,m ) ² , which is the square of the reference reflectance at the mth temperature point; Reference reflectance at the mth temperature point: y* _25,m ＝c ₀ +c ₁ D _R,m +c ₂ (D _R,m ) ² +c ₃ D _G,m +c ₄ (D _G,m ) ² +c ₅ D _B,m + c ₆ (D _B,m ) ² .

Images