CN101884306A

CN101884306A - Animal behavior memory test maze device

Info

Publication number: CN101884306A
Application number: CN2010102268810A
Authority: CN
Inventors: 李建民
Original assignee: XUE CHENGJING
Current assignee: XUE CHENGJING
Priority date: 2010-07-15
Filing date: 2010-07-15
Publication date: 2010-11-17

Abstract

The invention discloses an animal behavior memory test maze device which comprises a main body that is a big box made of a transparent organic glass plate, wherein a center platform is placed in the center of the big box, movable food boxes are respectively placed at four bottom corners of the outer part of the big box, a channel is arranged between each food box and the big box, the inlet of the channel of each food box is provided with an opaque door which can be pushed open, the middle floor in each food box is provided with a small disk; and bags consisting of four pellets are respectively placed on gaps between the floors in the four food boxes and the bottom of the disk. Different patterns of vertical plates and illuminating devices are arranged close to four side surfaces of the outside of the big box, an opaque cover covers outside the big box, the food boxes, the pattern vertical plates and the illuminating devices, and the top end of the cover is provided with a camera. The invention can solve the technical problem of existing limit of a simple experiment observing target in a traditional test for an animal learning memory or cognitive function.

Description

Animal Behavior Science recall tests labyrinth device

Technical field

The present invention relates to a kind of learning and memory testing tool that is used for Animal Behavior Science research.

Background technology

At present, in the research of animal learning memory or cognitive function, the labyrinth is the most frequently used behaviouristics monitoring device that arrives.Labyrinth experiment device characteristics commonly used are as follows: the space learning memory that T-labyrinth, radial labyrinth, Morris water maze are usually used in zoologizeing.Diving tower experiment, shuttle box experiment etc. belong to operant conditioned reflex, and wherein the shuttle box experiment is comparatively commonly used in the behaviouristics experiment, and this method can be observed passive and initiatively avoidant property reaction simultaneously.T-labyrinth, Y-labyrinth are used for the discrimination learning test.The impulsion that elevated plus-maze test has produced animal to probe into has simultaneously caused the conflict behavior of " probing into-avoid " with frightened, can reflect the anxiety of animal preferably.Emotional reactions such as active behavior, exploratory behavior and depression of spacious the main reflection of experiment animal or vigilance.Above behaviouristics method can the observational study object behaviouristics change, the ability and the variation of learning and memory, the learning and memory situation of evaluation studies object on the whole.Yet above behaviouristics method is because the limitation of the simple experiment object observing at the beginning of the design can't fully satisfy the new detection theory that continues to bring out.

Along with the progress of research to learning and memory, the new theory of various learning and memories continues to bring out, and new test model and advanced experimental technique are applied to research, gives the new challenge of having researched and proposed of learning and memory.Therefore, how traditional behaviouristics method is combined with up-to-date technological means, for the research of learning and memory provides science more, accurately, data become the problem that behaviouristics research field from now on must solve intuitively.

Summary of the invention

The purpose of this invention is to provide a kind of Animal Behavior Science recall tests labyrinth device, to solve in the test of traditional animal learning memory or cognitive function the limitation technical problem of the simple experiment object observing of existence.

A kind of Animal Behavior Science recall tests of the present invention labyrinth device, main body is a big box that the transparent poly (methyl methacrylate) plate of usefulness is made, and is positioned at central platform of middle placement of big box; A movably hamper is respectively placed at the place, four base angles of big box outside, and this hamper and big box have a passage, and there is an opaque door that can be open at the feeder connection place of each hamper, and is provided with a roundel on the intermediate floor in each hamper; Gap location between being placed at the bottom of floor in described four hampers and the disk respectively by four bags of forming of food balls; Four sides are closed on different pattern riser and lighting device are set outside big box, have a lighttight cover to cover on above-mentioned big box, hamper, pattern riser and the lighting device outside, lay camera on the cover top, and described camera is connected with external connection monitors.The mounting means that is open of the opaque door in the feeder connection place of described hamper can be taked hinged connected mode by the feeder connection place at door upside and hamper, or other ways of connecting.

The floor of described big box and hamper and the upper surface of central platform are all adopted the shielding material of anti-electromagnetic interference or are compounded with the shielding material layer of anti-electromagnetic interference.

When before experiment, being placed on animal on the central platform of placing the centre in the big box, place a quadrilateral frame that stops animal on the central platform.

Described central platform height is adjusted according to animal species.

The invention has the beneficial effects as follows: the present invention is effective learning and memory testing tool through experimental results demonstrate, can assess simultaneously with reference to memory and working memory.Every animal is finished in used time of each experiment, every the each experiment of animal the used time of first food from beginning to eating up; Enter no box number of times in kind, repeat to enter and eaten up food hamper number of times etc., can observe the moving reaction of activity situation, tense situation, feelings of animal and the situation of learning and memory simultaneously as detection index with reference to memory and working memory.The present invention combines the advantage of above all kinds of experimental techniques, avoided various rewards and punishments to stimulate animal excitement, the uneasiness that causes, can be in conjunction with the electric physiology and the magnetic variationization that adopt in the activity of neural electrophysiological technique and electromagnetic technique record behaviouristics, making more science, reliable of result, is the device that a kind of comparatively desirable detection learning and memory changes.

Description of drawings

Fig. 1 is a structural principle schematic diagram of the present invention.

Fig. 2 is the structural representation that central platform of the present invention is placed quadrilateral frame.

Embodiment

Structure of the present invention is referring to Fig. 1, and main body is a big box 1 that the transparent poly (methyl methacrylate) plate of usefulness is made, and a central platform 2 is put in the centre, and whether the motor function of observing animal is normal.Box 1 outside four jiaos respectively place movably hamper 3-1,3-2,3-3, a 3-4, be convenient to place the food ball.The porch of each hamper 3-1,3-2,3-3,3-4, one opaque door 5-1,5-2,5-3, the 5-4 that can be open arranged, animal can be looked for food by this turnover, puts a roundel 4-1,4-2,4-3,4-4 on each hamper 3-1,3-2,3-3, the 3-4 intermediate floor.Place the circle food ball of a 9～10mg among each roundel 4-1,4-2,4-3, the 4-4.Make by the shielding material attaching of anti-electromagnetic interference the floor of big box 1 and hamper 3-1,3-2,3-3,3-4 and the upper surface of central platform 2, to reduce the interference of electromagnetic environment.

The sign of different pattern (●, ▲, ★, ■) is laid at the outer four sides of box, for animal provides corresponding spatial cues.Among Fig. 16 is the risers with ★ pattern, the 7th, have ● the riser of pattern, and the 8th, have the riser of ■ pattern.For the ease of observing interior of the present invention, have ▲ riser of pattern is not shown in Fig. 1.Cover with the black cloth curtain around the device, lighting device is placed in the fixed position all around, gives soft light illumination, keeps stable environment, is beneficial to observe, monitor.Camera is laid on the cover top, external connection monitors.Described cover does not illustrate in Fig. 1 yet.The experimenter passes through the activity of monitor observed and recorded animal outside cover.All data all use the calculator monitoring of software to write down, store.Described monitoring of software is the application program of this matched with devices exploitation, can write down multiple test parameters.

Before each test, with an opaque box (the high 17cm of 9 * 9cm is long) animal is transported to from breeding cage on the central platform 2, animal is placed on the central platform 2, add a quadrilateral frame 9 that stops animal, take quadrilateral frame 9 after 30 seconds away, begin experiment.Place the structure of quadrilateral frame 9 on the central platform 2 referring to shown in Figure 2.

Claims

1. Animal Behavior Science recall tests labyrinth device, it is characterized in that: its main body is a big box that the transparent poly (methyl methacrylate) plate of usefulness is made, be positioned at big box in the middle of place a central platform; A movably hamper is respectively placed at the place, four base angles of big box outside, and this hamper and big box have a passage, and there is an opaque door that can be open at the feeder connection place of each hamper, and is provided with a roundel on the intermediate floor in each hamper; Gap location between being placed at the bottom of floor in described four hampers and the disk respectively by four bags of forming of food balls; Four sides are closed on different pattern riser and lighting device are set outside big box, have a lighttight cover to cover on above-mentioned big box, hamper, pattern riser and the lighting device outside, lay camera on the cover top, and described camera is connected with external connection monitors.

2. a kind of Animal Behavior Science recall tests according to claim 1 labyrinth device is characterized in that: the floor of described big box and hamper and the upper surface of central platform are all adopted the shielding material of anti-electromagnetic interference or are compounded with the shielding material layer of anti-electromagnetic interference.

3. a kind of Animal Behavior Science recall tests according to claim 1 labyrinth device is characterized in that: when being placed on animal on the central platform of placing the centre in the big box before experiment, place a quadrilateral frame that stops animal on the central platform.

4. a kind of Animal Behavior Science recall tests according to claim 1 labyrinth device, it is characterized in that: described central platform height is adjusted according to animal species.